enrichment and molecular characterization of chloropicrin- and metam-sodium-degrading microbial communities.chloropicrin (cp) and metam sodium are commonly used as fumigants in agricultural soils in order to provide effective control of nematodes, soil-borne pathogens, and weeds in preparation for planting of high-value cash crops. repeated application of these compounds to agricultural soils for many years may result in the enrichment of microorganisms capable of degrading them. in this study, a microcosm-enrichment approach was used to investigate bacterial populations that may be components of meta ...200415309337
isolation of a cadmium-releasing bacterium and characterization of its novel protease.microorganisms were screened for their ability to release cadmium from scallop hepatopancreas, which is the main residue after removing of the edible parts of scallop. the isolated strain, 23-0-11, identified as arthrobacter nicotinovorans, secreted a protease which released cadmium from scallop hepatopancreas into the liquid medium. the molecular mass of the enzyme was estimated to be 27 kda. the sequence of the 15 n-terminal amino acids of the protease showed no close similarity with any other ...200415322344
isolation of coryneform bacteria from blood cultures of patients at a university hospital in saudi arabia.coryneform bacteria have been increasingly recognized as opportunistic pathogens in recent years. the aim of this study is to identify and determine the antimicrobial susceptibility of coryneform bacteria isolated from blood cultures of patients seen at king khalid university hospital (kkuh), riyadh, kingdom of saudi arabia and review the literature.200415322601
differential inhibition of six copper amine oxidases by a family of 4-(aryloxy)-2-butynamines: evidence for a new mode of inactivation.a series of compounds derived from a previously identified substrate analogue of copper amine oxidases (cuaos) (shepard et al. (2002) eur. j. biochem. 269, 3645-3658) has been screened against six different cuaos with a view to designing potent and selective inhibitors. the substrate analogues investigated were 4-(1-naphthyloxy)-2-butyn-1-amine, 4-(2-methylphenoxy)-2-butyn-1-amine, 4-(3-methylphenoxy)-2-butyn-1-amine, 4-(4-methylphenoxy)-2-butyn-1-amine, and 4-phenoxy-2-butyn-1-amine. these comp ...200415323556
crystal structure of t-protein of the glycine cleavage system. cofactor binding, insights into h-protein recognition, and molecular basis for understanding nonketotic hyperglycinemia.the glycine cleavage system catalyzes the oxidative decarboxylation of glycine in bacteria and in mitochondria of animals and plants. its deficiency in human causes nonketotic hyperglycinemia, an inborn error of glycine metabolism. t-protein, one of the four components of the glycine cleavage system,is a tetrahydrofolate dependent aminomethyltransferase. it catalyzes the transfer of the methylene carbon unit to tetrahydrofolate from the methylamine group covalently attached to the lipoamide arm ...200415355973
amine-synthesizing enzyme n-substituted formamide deformylase: screening, purification, characterization, and gene cloning.n-substituted formamide was produced through the hydration of an isonitrile by isonitrile hydratase in the isonitrile metabolism. the former compound was further degraded by a microorganism, strain f164, which was isolated from soil through an acclimatization culture. the n-substituted formamide-degrading microorganism was identified as arthrobacter pascens. the microbial degradation was found to proceed through an enzymatic reaction, the n-substituted formamide being hydrolyzed to yield the cor ...200415358859
does smearing inoculum reflect the bacterial composition of the smear at the end of the ripening of a french soft, red-smear cheese?the microbial community composition and dynamics during the production of a french soft, red-smear cheese were investigated. the colonization efficiency of the smearing inoculum was followed, and the parts played by the inoculum used and the resident microflora were tentatively estimated. single-strand conformation polymorphism analysis (sscp) was applied to 2 productions of a soft, red-smear cheese produced by the same dairy plant at 4-mo intervals. microbial composition of the different cheese ...200415377597
crystal structure of bacterial inorganic polyphosphate/atp-glucomannokinase. insights into kinase evolution.inorganic polyphosphate (poly(p)) is a biological high energy compound presumed to be an ancient energy carrier preceding atp. several poly(p)-dependent kinases that use poly(p) as a phosphoryl donor are known to function in bacteria, but crystal structures of these kinases have not been solved. here we present the crystal structure of bacterial poly(p)/atp-glucomannokinase, belonging to gram-positive bacterial glucokinase, complexed with 1 glucose molecule and 2 phosphate molecules at 1.8 a res ...200415377666
solvent toxicity in organic-aqueous systems analysed by multivariate analysis.the effect of several solvents present in a biphasic reaction system on cells of rhodococcus erythropolis dcl14, xanthobacter py2, arthrobacter simplex and mycobacterium sp. nrrl b-3805 was evaluated. these four strains have been widely exploited, from bioremediation to the production of fine chemicals, in two-phase reaction media. the solvents tested were ethyl butyrate, n-hexane, cyclohexane, iso-octane, n-dodecane, dmso, bis(2-ethylhexyl) phthalate and fluorinert fc-70. the cell population wa ...200415378340
the structure of resting bacterial populations in soil and subsoil permafrost.the structure of individual cells in microbial populations in situ of the arctic and antarctic permafrost was studied by scanning and transmission electron microscopy methods and compared with that of cyst-like resting forms generated under special conditions by the non-spore-forming bacteria arthrobacter and micrococcus isolated from the permafrost. electron microscopy examination of microorganisms in situ revealed several types of bacterial cells having no signs of damage, including "dwarf" cu ...200415383239
cloning, expression and characterization of a sialidase gene from arthrobacter ureafaciens.sialidases have recently been used in the processing of clinically relevant asialoproteins. the arthrobacter ureafaciens sialidase (ec exhibits broad substrate specificity and is often used in such applications. we have employed an expression cloning strategy to isolate the a. ureafaciens sialidase. the clone encodes a 990-amino-acid 104 kda open-reading-frame protein containing three domains: an n-terminal catalytic domain, a linker domain with an immunoglobulin-like fold and a c-term ...200515461582
a functional screen identifies lateral transfer of beta-glucuronidase (gus) from bacteria to fungi.lateral gene transfer (lgt) from prokaryotes to microbial eukaryotes is usually detected by chance through genome-sequencing projects. here, we explore a different, hypothesis-driven approach. we show that the fitness advantage associated with the transferred gene, typically invoked only in retrospect, can be used to design a functional screen capable of identifying postulated lgt cases. we hypothesized that beta-glucuronidase (gus) genes may be prone to lgt from bacteria to fungi (thought to la ...200515483318
transformation of 2-aminoacetophenone to ( s) -2-amino-1-phenylethanol by arthrobacter sulfureus.a new isolate of arthrobacter sulfureus , when incubated at 50 g resting cells (dry cell wt) l(-1) with 50 g glucose l(-1) and 1 g 2-aminoacetophenone l(-1) in 50 mm potassium buffer (ph 7, 4 ml) at 30 degrees c, produced ( s )-2-amino-1-phenylethanol (e.e. >99%) with 75% yield in 6 h.200415483382
effect of chromium(vi) action on arthrobacter oxydans.arthrobacter species is of interest because of its high potential for bioremediation. bacteria can detoxify chromium, by either reduction or accumulation inside the bacteria and/or absorption of chromium(vi) (crvi) on their surface, and efflux pump. the possible pathway of cr(vi) reduction by arthrobacter oxydans isolated from columbia basalt rocks at a us doe highly contaminated site (usa) has been considered in the present study. ftir absorption spectroscopy showed that these bacteria reduce c ...200415486705
genetic engineering of glycinebetaine synthesis in tomato protects seeds, plants, and flowers from chilling damage.tomato (lycopersicon esculentum mill.) plants, which normally do not accumulate glycinebetaine (gb), are susceptible to chilling stress. exposure to temperatures below 10 degrees c causes various injuries and greatly decreases fruit set in most cultivars. we have transformed tomato (cv. moneymaker) with a chloroplast-targeted coda gene of arthrobacter globiformis, which encodes choline oxidase to catalyze the conversion of choline to gb. these transgenic plants express coda and synthesize cholin ...200415500464
screening procedures for selecting rhizobacteria with biocontrol effects upon fusarium verticillioides growth and fumonisin b1 production.screening is a critical step in the discovery of microbial agents that can exert biological control of fusarium verticillioides at the root level. the objectives of this research were to determine the utility of a niche overlap index to realise the first screening of maize rhizobacterial isolates during different water activities. studies were conducted to evaluate various methods for second screening with different modes of action. the antifungal activity of bacterial isolates through antibiosi ...200415501652
[ultrastructure of resting cells of some non-spore-forming bacteria].using electron microscopy (ultrathin sections and freeze-fractures), we investigated the ultrastructure of the resting cells formed in the cultures of micrococcus luteus, arthrobacter globiformis, and pseudomonas aurantiaca under conditions of prolonged incubation (up to 9 months). these resting cells included cyst-like forms that were characterized by complex cell structure and the following ultrastructural properties: (i) a thickened or multiprofiled cell wall (cw), typically made up of a laye ...200415521179
dioxygenases without requirement for cofactors and their chemical model reaction: compulsory order ternary complex mechanism of 1h-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase involving general base catalysis by histidine 251 and single-electron oxidation of the substrate dianion.1h-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase (hod) is a cofactor-less dioxygenase belonging to the alpha/beta hydrolase fold family, catalyzing the cleavage of 1h-3-hydroxy-4-oxoquinaldine (i) and 1h-3-hydroxy-4-oxoquinoline (ii) to n-acetyl- and n-formylanthranilate, respectively, and carbon monoxide. bisubstrate steady-state kinetics and product inhibition patterns of hodc, the c69a protein variant of hod, suggested a compulsory-order ternary-complex mechanism, in which binding of the organic ...200415533053
fluorinated phenylcyclopropylamines. 2. effects of aromatic ring substitution and of absolute configuration on inhibition of microbial tyramine oxidase.a series of para-substituted diastereopure cis- and trans-2-fluoro-2-arylcyclopropylamines were synthesized and these were investigated as inhibitors of microbial tyramine oxidase from arthrobacter sp. all compounds were shown to be competitive inhibitors of this enzyme. the nature of the para-substituents in the more potent trans-isomer (cis-relationship between fluorine and the amino group) of 2-fluoro-2-arylcyclopropylamine influenced the inhibitory potency in a consistent fashion. thus, elec ...200415537343
arthrobacter psychrophenolicus sp. nov., isolated from an alpine ice cave.on the basis of phenotypic, genotypic and chemotaxonomic characteristics, a novel species belonging to the genus arthrobacter is described. a facultatively psychrophilic bacterium, strain ag31(t), was isolated from an alpine ice cave. the aerobic, gram-positive, non-spore-forming, non-motile strain exhibited a rod-coccus growth cycle and produced a yellow pigment. good growth and phenol biodegradation occurred at a temperature range of 1-25 degrees c. up to 10 mm phenol was utilized as a sole ca ...200415545436
arthrobacter gangotriensis sp. nov. and arthrobacter kerguelensis sp. nov. from antarctica.two coryneform bacteria were isolated from a penguin rookery soil sample collected in antarctica, near the indian station dakshin gangotri (strain lz1y(t)), and from sea water from kerguelen island, antarctica (strain kgn15(t)). they have morphological and chemotaxonomic properties (peptidoglycan a4alpha type; major menaquinones mk-8, mk-9 and mk-10; predominant fatty acids anteiso-c(15 : 0) and anteiso-c(17 : 0)) that are characteristic of members of the genus arthrobacter. the isolates shared ...200415545486
degradation of 4-chlorophenol at low temperature and during extreme temperature fluctuations by arthrobacter chlorophenolicus a6.low average temperatures and temperature fluctuations in temperate soils challenge the efficacy of microbial strains used for clean up of pollutants. in this study, we investigated the cold tolerance of arthrobacter chlorophenolicus a6, a microorganism previously shown to degrade high concentrations of 4-chlorophenol at 28 degrees c. luciferase activity from a luc-tagged derivative of the strain (a6l) was used to monitor the metabolic status of the population during 4-chlorophenol degradation. t ...200415546043
stimulation of phagocytosis of influenza virus-infected cells through surface desialylation of macrophages by viral neuraminidase.cells infected with influenza a virus undergo apoptosis and become susceptible to phosphatidylserine-mediated phagocytosis by macrophages. this study was undertaken to elucidate the mechanism underlying our previous finding that the activity of viral neuraminidase (na) is required for efficient phagocytosis. treatment of macrophages, not influenza virus-infected cells, with arthrobacter ureafaciens na or virus-infected cells expressing viral na augmented the level of phagocytosis of virus-infect ...200415557745
bacterial diversity of a soil sample from schirmacher oasis, antarctica.the bacterial diversity of a soil sample collected in the vicinity of lake zub, schirmacher oasis, antarctica, was determined both by establishing pure colonies of culturable bacteria and by cloning the total 16s rdna of the soil and establishing the phylogeny of the clones. analysis of the 16s rrna gene clones indicated that the bacteria belonged to the classes alpha-proteobacteria, beta-proteobacteria, gamma-proteobacteria, gemmatimonas, bacteriodetes, actinobacteria, chloroflexi and chlamydia ...200415559969
a novel gamma-n-methylaminobutyrate demethylating oxidase involved in catabolism of the tobacco alkaloid nicotine by arthrobacter nicotinovorans pao1.nicotine catabolism, linked in arthrobacter nicotinovorans to the presence of the megaplasmid pao1, leads to the formation of gamma-n-methylaminobutyrate from the pyrrolidine ring of the alkaloid. until now the metabolic fate of gamma-n-methylaminobutyrate has been unknown. pao1 carries a cluster of orfs with similarity to sarcosine and dimethylglycine dehydrogenases and oxidases, to the bifunctional enzyme methylenetetrahydrofolate dehydrogenase/cyclohydrolase and to formyltetrahydrofolate defo ...200415606755
bacterial diversity in spent mushroom compost assessed by amplified rdna restriction analysis and sequencing of cultivated isolates.spent mushroom compost (smc) is the residual by-product of commercial agaricus spp. cultivation, and it is mainly composed of a thermally treated cereal straw/animal manure mixture colonized by the fungal biomass. research on the valorization of this material is mainly focusing on its use as soil conditioner and plant fertilizer. an investigation of the bacterial diversity in smc was performed using molecular techniques in order to reveal the origin of smc microflora and its potential effect on ...200415612633
cyclic tetrasaccharide-synthesizing enzymes from arthrobacter globiformis a19.a bacterial strain arthrobacter globiformis a19 producing cyclic tetrasaccharide (cts) was isolated from soil. the enzymes, 6-alpha-glucosyltransferase (6gt) and 3-alpha-isomaltosyltransferase (imt), involved in the synthesis of cts were purified to homogeneity. the molecular and enzymatic properties of imt from a. globiformis were similar to those of enzymes from bacillus globisporus c11 and n75. arthrobacter 6gt had a smaller molecular mass of 108 kda and a higher optimum ph of 8.4 than the en ...200415618624
diversity of biphenyl degraders in a chlorobenzene polluted aquifer.biphenyl degrading bacteria (40 strains) have been isolated along a gradient of chlorobenzene pollution from an aquifer which did not contain any pcb to answer the question of how metabolic/catabolic abilities exist in ecosystems that have not been stressed with the relevant substrates is important for intrinsic bioremediations. only few of the isolates were characterized by 16s rrna gene sequence analyses as pseudomonas species while the majority were gram-positive, belonging to the order actin ...200515620745
influenza virus activates human immunodeficiency virus type-1 gene expression in human cd4-expressing t cells through an nf-kappab-dependent pathway.influenza virus infection can cause severe complications in human immunodeficiency virus type-1 (hiv-1)-infected individuals leading to an increased risk of complications and death compared to that seen in uninfected individuals. we assessed the capacity of influenza virus (flu) to modulate transcription of the hiv-1 long terminal repeat (ltr) in human cd4+ t cells. we found that flu is able to promote expression of both the transiently transfected and stably integrated hiv-1 ltr-driven reporter ...200515639653
diversity of green-like and red-like ribulose-1,5-bisphosphate carboxylase/oxygenase large-subunit genes (cbbl) in differently managed agricultural soils.a pcr-based approach was developed to detect ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco) form i large-subunit genes (cbbl) as a functional marker of autotrophic bacteria that fix carbon dioxide via the calvin-benson-bassham cycle. we constructed two different primer sets, targeting the green-like and red-like phylogenetic groups of cbbl genes. the diversity of these cbbl genes was analyzed by the use of three differently managed agricultural soils from a long-term field experiment. ...200515640185
arthrobacter bergerei sp. nov. and arthrobacter arilaitensis sp. nov., novel coryneform species isolated from the surfaces of cheeses.fourteen isolates of two different bacterial species isolated from the surface of smear-ripened cheeses were found to exhibit many characteristics of the genus arthrobacter. the isolates were aerobic, gram-positive, catalase-positive, non-spore-forming and non-motile. the cell-wall peptidoglycan contained lysine, alanine and glutamic acid. rrs sequence analysis indicated that the new isolates re117t and ca106t are closely related to the arthrobacter nicotianae group and showed highest sequence s ...200515653918
optimum culture conditions for the production of n-substituted formamide deformylase by arthrobacter pascens f164.we investigated the optimum culture conditions for the production of a novel enzyme, n-substituted formamide deformylase, which acts mainly on n-benzylformamide, in arthrobacter pascens f164. the highest enzyme activity was obtained when this strain f164 was cultivated in a synthetic medium with n-benzylformamide as sole nitrogen source. this deformylase was found to be an inducible enzyme depending on n-benzylformamide.200515665493
isolation and characterization of a fructosyl-amine oxidase from an arthrobacter arthrobacter sp. was isolated that, when induced by fructosyl-valine, expressed a fructosyl-amine oxidase (faod) that was specific for alpha-glycated amino acids. the n-terminal amino acid sequence of the purified oxidase was determined and used to design oligonucleotides to amplify the gene by inverse pcr. expression of the gene in escherichia coli produced 0.23 units faod per mg protein, over 30-fold greater than native expression levels, with properties almost indistinguishable from the na ...200515685416
chemoenzymatic synthesis of cd52 glycoproteins carrying native n-glycans.a facile synthesis of homogeneous cd52 glycoproteins carrying native n-glycans was achieved using an endolycosidase-catalyzed oligosaccharide transfer as the key step. the synthesis consists of two steps: the solid phase synthesis of glcnac-cd52 and the transfer of a high-mannose type or complex type n-glycan from man(9)glcnac(2) asn or a sialglycopeptide to the glcnac-cd52, under the catalysis of the endo-beta-n-acetylglucosaminidases from arthrobacter (endo-a) and mucor hiemalis (endo-m), resp ...200515686882
identification of large linear plasmids in arthrobacter spp. encoding the degradation of quinaldine to anthranilate.arthrobacter nitroguajacolicus rü61a, which utilizes quinaldine as sole source of carbon and energy, was shown to contain a conjugative linear plasmid of approximately 110 kb, named pal1. it exhibits similarities with other linear plasmids from actinomycetales in that it has proteins covalently attached to its 5' ends. southern hybridization with probes for the genes encoding quinaldine 4-oxidase and n-acetylanthranilate amidase indicated that pal1 contains the gene cluster encoding the degradat ...200515699198
purification and characterization of an esterase hydrolyzing monoalkyl phthalates from micrococcus sp. esterase that specifically hydrolyzes medium-chain (c(3)-c(5)) monoalkyl phthalates was purified from phthalate-grown micrococcus sp. ygj1. the enzyme activity was split into two fractions by hydrophobic chromatography on phenyl sepharose, and the enzymes were purified to homogeneity from each fraction. the purified enzymes showed similar properties with respect to molecular mass (60 kda), subunit molecular mass (27 kda), n-terminal amino acid sequence, optimal ph (about 7.5), temperature-dep ...200515713880
desulphurization of dibenzothiophene and diesel oils by study the desulphurization of dibenzothiophene (dbt), a recalcitrant thiophenic component of fossil fuels, by two bacteria namely rhodococcus sp. and arthrobacter sulfureus isolated from oil-contaminated soil/sludge in order to use them for reducing the sulphur content of diesel oil in compliance with environmental regulations.200515715638
molecular cloning of levan fructotransferase gene from arthrobacter ureafaciens k2032 and its expression in escherichia coli for the production of difructose dianhydride clone and overexpress a novel levan fructotransferase gene lfta from arthrobacter ureafaciens k2032.200515715649
high-level expression of a novel amine-synthesizing enzyme, n-substituted formamide deformylase, in streptomyces with a strong protein expression system.n-substituted formamide deformylase (nfda) from arthrobacter pascens f164 is a novel deformylase involved in the metabolism of isonitriles. the enzyme catalyzes the deformylation of an n-substituted formamide, which is produced from the corresponding isonitrile, to yield the corresponding amine and formate. the nfda gene from a. pascens f164 was cloned into different types of expression vectors for escherichia coli and streptomyces strains. expression in e. coli resulted in the accumulation of a ...200515721791
microbiological degradation of pentane by immobilized cells of arthrobacter sp.the increasing production of several plastics such as expanded polystyrene, widely used as packaging and building materials, has caused the release of considerable amounts of pentane employed as an expanding agent. today many microorganisms are used to degrade hydrocarbons in order to minimize contamination caused by several industrial activities. the aim of our work was to identify a suitable microorganism to degrade pentane. we focused our attention on a strain of arthrobacter sp. which in a s ...200515727150
arthrobacter scleromae sp. nov. isolated from human clinical specimens.a gram-positive, coryneform bacterium was isolated from swollen scleromata of a dermatosis patient. an analysis of its phenotypic, chemotaxonomic, and genotypic characteristics showed that this bacterium is closely associated with arthrobacter oxydans and arthrobacter polychromogenes but that it belongs to a distinct species, for which the name arthrobacter scleromae sp. nov. is proposed.200515750131
the structure of hormaomycin and one of its all-peptide aza-analogues in solution: syntheses and biological activities of new hormaomycin analogues.four new aza-analogues of hormaomycin 1, a secondary metabolite with interesting biological activities produced by streptomyces griseoflavus, were synthesized and subjected to preliminary tests of their antibiotic activity to provide new insights into the structure-activity relationship studies of this class of compounds. the solution structures of hormaomycin 1 and its aza-analogue 2 a were determined by nmr spectroscopy. the data exhibited a reasonably rigid conformation for both molecules, st ...200515754385
a strain of arthrobacter that tolerates high concentrations of nitrate.a gram-positive strain identified as arthrobacter globiformis cect 4500, tolerant to up to 1 m nitrate, was isolated from the grounds of a munitions factory. under strict aerobic conditions, this bacterium used a wide variety of c-sources to obtain the energy required for growth, which took place when the nitrate concentration in the medium was below 150 mm. cells of this bacterium growing in the absence of nitrate were seen as individual cells or forming pairs, whereas cells grown in the presen ...199715765585
polyphasic taxonomic study of strain ccm 2783 resulting in the description of arthrobacter stackebrandtii sp. nov.strain ccm 2783, previously classified as representing arthrobacter aurescens, was subjected to a polyphasic taxonomic study. 16s rrna gene sequence analysis and chemotaxonomic characteristics such as peptidoglycan type a3alpha lys-ala(2), major menaquinone mk-9(h(2)) and fatty acid composition confirmed assignment of the strain to the genus arthrobacter. the results of phylogenetic analysis, dna-dna relatedness experiments and physiological and chemotaxonomic characteristics indicate that ccm 2 ...200515774666
structure of beta-glucan oligomer from laminarin and its effect on human monocytes to inhibit the proliferation of u937 cells.we analyzed the human monocyte-stimulating ability of laminarin from eisenia bicyclis, lichenan from cetraria islandica, and their oligomers depolymerized with endo-1,3-beta-glucanase from arthrobacter sp. the respective beta-glucan oligomers with different degrees of polymerization (dp) were fractionated from hydrolytic products of laminarin and lichenan using gel-filtration chromatography. the monocyte-conditioned medium pre-cultured in the presence of a fraction of beta-glucan oligomer (dp>/= ...200515784984
rhizobacteria and their potential to control fusarium verticillioides: effect of maize bacterisation and inoculum density.fusarium verticillioides is the most important seed transmitted pathogen that infects maize. it produces fumonisins, toxins that have potential toxicity for humans and animals. control of f. verticillioides colonisation and systemic contamination of maize has become a priority area in food safety research. the aims of this research were (1) to characterise the maize endorhizosphere and rhizoplane inhabitant bacteria and fusarium spp., (2) to select bacterial strains with impact on f. verticillio ...200515803383
a microcosm study on bioremediation of p-nitrophenol-contaminated soil using arthrobacter protophormiae rkj100.p-nitrophenol (pnp), a toxic nitroaromatic compound, can build up in soils due to extensive usage of nitrophenolic pesticides and hence needs to be removed. arthrobacter protophormiae rkj100, a pnp-degrading organism, was used in this work to study factors affecting its growth, and then evaluated for its capacity to degrade pnp in soil microcosms. molasses (10%) treated with 0.1% potassium hexacyanoferrate was found to be a suitable and cheap carbon source for inoculum preparation. induction stu ...200515806356
purification and characterization of a novel sialidase from a strain of arthrobacter nicotianae.the nonpathogenic strain arthrobacter nicotianae produces two sialidase isoenzymes, na1 and na2, with molecular masses of 65 kda and 54 kda, respectively, as determined by 10% sds-polyacrylamide gel electrophoresis. na1 and na2 exhibit maximum activities at ph 4 and 5, and both show clear thermal optima at 40 degrees c. they are stable at temperatures up to 50 degrees c. the critical temperatures (t (c) = 50 degrees c and 51 degrees c) for the two isoenzymes were determined by fluorescence spect ...200515809338
arthrobacter ardleyensis sp. nov., isolated from antarctic lake sediment and deep-sea sediment.three psychrotrophic arthrobacter strains, isolated from antarctic lake sediment (an24, an25t) and deep-sea sediment (zx6) were studied. their 16s rrna gene sequences showed highest similarities (97.0-97.9%) with those of a. nicotianae and a. protophormiae. all three strains underwent rod-coccus morphological change, had high mol% g+c content, were aerobic to slightly anaerobic, and grew between 0 degrees c and 30 degrees c, with optimal growth temperature around 25 degrees c. the cell wall pept ...200515834596
characterization of pmfr, the transcriptional activator of the pao1-borne puru-mabo-fold operon of arthrobacter nicotinovorans.nicotine catabolism by arthrobacter nicotinovorans is linked to the presence of the megaplasmid pao1. genes involved in this catabolic pathway are arranged on the plasmid into gene modules according to function. during nicotine degradation gamma-n-methylaminobutyrate is formed from the pyrrolidine ring of nicotine. analysis of the pao1 open reading frames (orf) resulted in identification of the gene encoding a demethylating gamma-n-methylaminobutyrate oxidase (mabo). this gene was shown to form ...200515838033
biodegradation of naphthalene-2-sulfonic acid present in tannery wastewater by bacterial isolates arthrobacter sp. 2ac and comamonas sp. 4bc.two bacterial strains, 2ac and 4bc, both capable of utilizing naphthalene-2-sulfonic acid (2-nsa) as a sole source of carbon, were isolated from activated sludges previously exposed to tannery wastewater. enrichments were carried out in mineral salt medium (msm) with 2-nsa as the sole carbon source. 16s rdna sequencing analysis indicated that 2ac is an arthrobacter sp. and 4bc is a comamonas sp. within 33 h, both isolates degraded 100% of 2-nsa in msm and also 2-nsa in non-sterile tannery wastew ...200515865148
depolymerisation and biodegradation of a synthetic tanning agent by activated sludges, the bacteria arthrobacter globiformis and comamonas testosteroni, and the fungus cunninghamella polymorpha.degradation of a synthetic tanning agent cnsf (a condensation product of 2-naphthalenesulfonic acid (2-nsa) and formaldehyde) by four activated sludges, two previously characterised bacterial strains, arthrobacter sp. 2ac and comamonas sp. 4bc, and the fungus cunninghamella polymorpha, was studied in batch culture at 25 degrees c by determining the changes in the concentrations of cnsf and its component monomers and oligomers (n2-n11). the loss of individual oligomers was correlated with the len ...200515865336
enhanced laminin binding by alpha-dystroglycan after enzymatic deglycosylation.carbohydrate modifications are clearly important to the function of alpha-dystroglycan but their composition and structure remain poorly understood. in the present study, we describe experiments aimed at identifying the alpha-dystroglycan oligosaccharides important for its binding to laminin-1 and carbohydrate-dependent mabs (monoclonal antibodies) iih6 and via4(1). we digested highly purified skeletal muscle alpha-dystroglycan with an array of linkage-specific endo- and exoglycosidases, which w ...200515865602
isolation and characterization of a diverse group of phenylacetic acid degrading microorganisms from pristine soil.a diverse range of microorganisms capable of growth on phenylacetic acid as the sole source of carbon and energy were isolated from soil. sixty six different isolates were identified and grouped according to 16s rrna gene rflp analysis. subsequent sequencing of 16s rdna from selected strains allowed further characterization of the phenylacetic acid degrading population isolated from soil. nearly half (30) of the isolates are bacillus species while the rest of the isolates are strains from a vari ...200515869782
substrate specificity and colorimetric assay for recombinant trzn derived from arthrobacter aurescens tc1.the trzn protein, which is involved in s-triazine herbicide catabolism by arthrobacter aurescens tc1, was cloned and expressed in escherichia coli as a his-tagged protein. the recombinant protein was purified via nickel column chromatography. the purified trzn protein was tested with 31 s-triazine and pyrimidine ring compounds; 22 of the tested compounds were substrates. trzn showed high activity with sulfur-substituted s-triazines and the highest activity with ametryn sulfoxide. hydrolysis of a ...200515870302
an enzymatically produced novel cyclic tetrasaccharide, cyclo-{-->6)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->6)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->} (cyclic maltosyl-(1-->6)-maltose), from starch.a bacterial strain m6, isolated from soil and identified as arthrobacter globiformis, produced a novel nonreducing oligosaccharide. the nonreducing oligosaccharide was produced from starch using a culture supernatant of the strain as enzyme preparation. the oligosaccharide was purified as a crystal preparation after alkaline treatment and deionization of the reaction mixture. the structure of the oligosaccharide was determined by methylation analysis, mass spectrometry, and (1)h and (13)c nmr sp ...200515882856
a brevibacillus choshinensis system that secretes cytoplasmic proteins.brevibacillus choshinensis has previously been shown to be a useful strain for the secretion of heterologous proteins via the sec secretory pathway. this pathway involves the secretion of proteins prior to folding, whereas the alternative tat (twin-arginine translocation) pathway enables pre-folded proteins to be secreted. we have modified the signal peptide of the brevibacillus expression vector pncmo2 to accommodate a sec avoidance signal as well as the twin arginines required for secretion vi ...200415925899
pot and field studies on bioremediation of p-nitrophenol contaminated soil using arthrobacter protophormiae rkj100.biodegradation of p-nitrophenol (pnp), a priority pollutant, was studied as a model system for bioremediation of sites contaminated with nitroaromatic/organic compounds. bioremediation of pnp-containing soil was first carried out in pots using immobilized and free cells of arthrobacter protophormiae rkj100 in order to ascertain the role of a suitable carrier material. results showed that stability of the introduced strain was enhanced upon immobilization and that the rate of pnp depletion decrea ...200515926586
the crystal structure of mlc, a global regulator of sugar metabolism in escherichia coli.mlc from escherichia coli is a transcriptional repressor controlling the expression of a number of genes encoding enzymes of the phosphotransferase system (pts), including ptsg and manxyz, the specific enzyme ii for glucose and mannose pts transporters. in addition, mlc controls the transcription of malt, the gene of the global activator of the mal regulon. the inactivation of mlc as a repressor is mediated by binding to an actively transporting ptsg (eiicb(glc)). here we report the crystal stru ...200515929984
production of amylase by arthrobacter psychrolactophilus.arthrobacter psychrolactophilus atcc 700733 grew with a doubling time of 1.5-2.3 h (22 degrees c) and produced up to 0.2 units/ml (soluble starch assay) of extracellular amylase in tryptic soy broth without dextrose (tsbwd) containing 0.5% or 1.0% (w/v) soluble starch or maltose as the fermentable substrate. time-course experiments in media containing soluble starch as substrate showed that amylolytic activity appeared in cultures at 24 h (after exponential growth had ceased), reached peak level ...200515931519
use of arthrobacter davidanieli as a live vaccine against renibacterium salmoninarum and piscirickettsia salmonis in salmonids.arthrobacter davidanieli (proposed species nomenclature) is a non-pathogenic gram-variable bacterium related to, but taxonomically distinct from, renibacterium salmoninarum, the aetiological agent of bacterial kidney disease (bkd). we have demonstrated that vaccination with live a. davidanieli is effective against bkd in atlantic salmon (salmo salar) showing above 80 relative percent survival in experimental challenge trials. good protection was also demonstrated in long-term field trials where ...200515962482
[cloning and expression of l-n-carbamoylase gene from arthrobacter bt801 in escherichia coli].hydantoin-utility-enzyme is widely used in enzymic production of various amino acids. one of its component, carbamoylase, is responsible for the conversion of n-carbamylamino acids to corresponding amino acids, which is crucial for the stereoselectivity and rate limiting. to improve the production of the enzyme, an l-n-carbamoylase gene from arthrobacter bt801, a hydantoinase producting strain being able to convert 5-benzylhydantoin to phenylalanine, was cloned into e. coli. the gene was highly ...200315966317
[purification and immobilization of chondroitinase from aeromonas sobria yh 311].chondroitinase has been used as an important tool in the study of the structure, function and distribution of glycosaminoglycans for many years. recently, the enzyme has been reported to be a potential enzyme for chemonucleolysis, an established treatment for intervertebral disc protrasion. in this paper, a chondroitinase had been purified from the culture supernatant of aeromonas sobria yh311 using a simple purification procedure of ammonium sulfate precipitation, qae-sephadex a50 ion exchange ...200415968993
[construction of gene library of arthrobacter bt801 and isolation & expression of hydantoinase gene].hydantoinase can be widely used in enzymic production of various amino acids. in order to obtain the hydantoinase genes in arthrobacter bt801, its chromatosomal dna is isolated and partialy digested with sau3a i to collect fragments of about 30kb. then, this fragment is inserted into the hpa i and pst i site of cosmid pkc505. the genomic library was thus constructed by packing in vitro with lambda phage package protein and transfecting e. coli dh5alpha. a positive transformant was selected from ...200315969007
kinetic studies of site-directed mutational isomalto-dextranase-catalyzed hydrolytic reactions on a 27 mhz quartz-crystal microbalance.a quartz-crystal microbalance (qcm) technique was applied to analyze effects of site-directed mutagenesis of a glycosidase (isomalto-dextranase) on the hydrolysis mechanism of the substrate binding (k(on), k(off), and k(d)) and the catalytic process (k(cat)), separately, by using a dextran-immobilized qcm in buffer solution. d266n, d198n, and d313n mutants, which are predicted as critical residues of the isomalto-dextranase hydrolytic activity, dramatically decreased the apparent enzyme activity ...200515996100
a novel mammalian expression system derived from components coordinating nicotine degradation in arthrobacter nicotinovorans pao1.we describe the design and detailed characterization of 6-hydroxy-nicotine (6hnic)-adjustable transgene expression (nice) systems engineered for lentiviral transduction and in vivo modulation of angiogenic responses. arthrobacter nicotinovorans pao1 encodes a unique catabolic machinery on its plasmid pao1, which enables this gram-positive soil bacterium to use the tobacco alkaloid nicotine as the exclusive carbon source. the 6hnic-responsive repressor-operator (hdnor-o(nic)) interaction, control ...200516002786
microbial synthesis of chiral amines by (r)-specific transamination with arthrobacter sp. knk168.arthrobacter sp. knk168 shows (r)-enantioselective transaminase [(r)-transaminase] activity, which converts prochiral ketones into the corresponding chiral (r)-amines in the presence of an amino donor. the cultural conditions and reaction conditions for asymmetric synthesis of chiral amines with this microorganism were examined. the transaminase was inducible, and its production was enhanced by the addition of sec-butylamine and 3-amino-2,2-dimethylbutane to the culture medium. (r)-1-phenylethyl ...200616003558
[purification and characteristics of creatininase from arthrobacter sp].a creatininase produced from a arthrobacter sp. was purified 145-fold by a series of steps including heat treatment, ammonium sulfate precipitation, deae-cellulose ion-exchange and hydrophobic chromatography. the specific activity of the pure enzyme was 209u/mg. the subunit molecular mass of creatininase was estimated to be 33 700d by sds-page. the creatininase was stable in the ph range between 6.0 - 9.0 and below 60 degrees c . its km value for creatinine was estimated to be 21.14 mmol/l. the ...200516013484
six novel arthrobacter species isolated from deteriorated mural paintings.a group of 21 bacterial strains was isolated from samples of biofilm formation in the servilia tomb (necropolis of carmona, spain) and the saint-catherine chapel (castle at herberstein, austria). a polyphasic taxonomic study of these isolates, including morphological, biochemical and chemotaxonomic characterization, rep-pcr fingerprinting, 16s rrna gene sequence analysis, dna base ratio and dna-dna relatedness studies, allocated them to the genus arthrobacter. the isolates represent six novel sp ...200516014466
crystal structure of dmgo provides a prototype for a new tetrahydrofolate-binding fold.the crystal structure of dmgo (dimethylglycine oxidase) from arthrobacter globiformis in complex with folate compounds has revealed a novel thf (tetrahydrofolate)-binding fold [leys, basran and scrutton (2003) embo j. 22, 4038-4048]. this fold is widespread among folate-binding proteins. the crystal structures of aminomethyltransferase (t-protein), ygfz and trme all reveal similar thf-binding folds despite little similarity in sequence or function. the thf-binding site is highly specific for red ...200516042597
role of the n-terminal domain of endoinulinase from arthrobacter sp. s37 in regulation of enzyme catalysis.endoinulinase from arthrobacter sp. s37 (enia), a member of the glycoside hydrolase family 32, is unique in that, unlike other members of the family, it contains a 250-residue n-terminal domain including a "laminin-g like jelly-roll" fold. this unique n-terminal domain is here suggested to be involved in dimerization and catalysis. the essentially inactive nature of enzymes produced by n-terminal truncation (delta15, delta45, delta70, and delta250) supported the pivotal role of this unique domai ...200516046445
removal of high concentration of nh3 and coexistent h2s by biological activated carbon (bac) biotrickling filter.high efficiency of nh3 and h2s removal from waste gases was achieved by the biotrickling filter. granular activated carbon (gac), inoculated with arthrobacter oxydans ch8 for nh3 removal and pseudomonas putida ch11 for h2s removal, was used as packing material. under conditions in which 100% h2s was removed, extensive tests to eliminate high concentrations of nh3 emission-including removal characteristics, removal efficiency, and removal capacity of the system-were performed. the results of the ...200516051088
genetic diversity of culturable bacteria in oil-contaminated rhizosphere of galega orientalis.a collection of 50 indigenous meta-toluate tolerating bacteria isolated from oil-contaminated rhizosphere of galega orientalis on selective medium was characterized and identified by classical and molecular methods. 16s rdna partial sequencing showed the presence of five major lineages of the bacteria domain. gram-positive rhodococcus, bacillus and arthrobacter and gram-negative pseudomonas were the most abundant genera. only one-fifth of the strains that tolerated m-toluate also degraded m-tolu ...200616055251
[removal of harmful admixtures from a surrogate atmospheric condensate of a closed habitat with the help of a cultivated bacterial association].tested was an idea to eliminate six harmful admixtures reside in a surrogate of atmospheric condensate of closed habitats by a cultivated bacterial association. the purpose was to select members of the bacterial associations that would assimilate acetone, acetic acid, ethanol, ethyl acetate, methylamine and ammonia as free, so immobilized on insoluble cellular polyvinylformal. the resulted association of three bacterial species showed the ability to transform into end products a six-component su ...200516078425
crystal structure of 6-hydroxy-d-nicotine oxidase from arthrobacter nicotinovorans.the crystal structure of 6-hydroxy-d-nicotine oxidase (ec was solved by x-ray diffraction analysis in three crystal forms at resolutions up to 1.9 a. the enzyme is monomeric in solution and also in the mother liquor but formed disulfide-dimers in all crystals. it belongs to the p-cresol methylhydroxylase-vanillyl-alcohol oxidase family and contains an fad covalently bound to the polypeptide. the covalent bond of this enzyme was the first for which a purely autocatalytic formation had be ...200516095622
preparation of the methyl ester of hyaluronan and its enzymatic degradation.a methyl ester of hyaluronan in which the carboxyl groups were fully esterified was prepared using trimethylsilyl diazomethane. this derivative, while not depolymerized by hyaluronan lyases or hyaluronan hydrolases, was a substrate for both chondroitin aci lyase (ec from flavobacterium heparinum and chondroitin acii lyase (ec from arthrobacter aurescens. the major product isolated in these depolymerization reactions was methyl alpha-l-threo-hex-4-enepyranosyluronate-(1-->3)-2-a ...200516098492
[expression of hydantoin hydrolase gene in escherichia coli].hydantoin hydrolase with responsibility for the ring opening of hydantoin is one of the components of hydantoin utility enzymes of arthrobacter bt801 which can convert 5-benzylhydantoin into l-phenylalanine. the expression of hydantoin hydrolase gene (hyuh) is very important in elucidation of mechanisms of bio-catalysis and its application in asymmetry synthesis of amino acids. to improve the production and activity of the enzyme, the hydantoin hydrolase gene was amplified by pcr and cloned into ...200416110958
[the microbial transformation of phenanthrene and anthracene].the transformation of phenanthrene and anthracene by rhodococcus rhodnii 135, pseudomonas fluorescens 26k, and arthrobacter sp. k3 is studied. twenty-one intermediates of phenanthrene and anthracene transformation are identified by hplc, mass spectrometry, and nmr spectroscopy. p. fluorescens 26k and arthrobacter sp. k3 are found to produce a wide range of intermediates, whereas r. rhodnii 135 oxidizes phenanthrene, resulting in the formation of a sole product, 3-hydroxyphenanthrene. putative tr ...200516119849
characteristics of the amylase of arthrobacter of the extracellular amylase produced by the psychrotrophic bacterium, arthrobacter psychrolactophilus, were determined for crude preparations and purified enzyme. the hydrolysis of soluble starch by concentrated crude preparations was found to be a nonlinear function of time at 30 and 40 degrees c. concentrates of supernatant fractions incubated without substrate exhibited poor stability at 30, 40, or 50 degrees c, with 87% inactivation after 21 h at 30 degrees c, 45% inactivation af ...200516133101
reinvestigation of metal ion specificity for quinone cofactor biogenesis in bacterial copper amine oxidase.the topa quinone (tpq) cofactor of copper amine oxidase is generated by copper-assisted self-processing of the precursor protein. metal ion specificity for tpq biogenesis has been reinvestigated with the recombinant phenylethylamine oxidase from arthrobacter globiformis. besides cu2+ ion, some divalent metal ions such as co2+, ni2+, and zn2+ were also bound to the metal site of the apoenzyme so tightly that they were not replaced by excess cu2+ ions added subsequently. although these noncupric m ...200516142901
reversible inhibition of copper amine oxidase activity by channel-blocking ruthenium(ii) and rhenium(i) molecular wires.molecular wires comprising a ru(ii)- or re(i)-complex head group, an aromatic tail group, and an alkane linker reversibly inhibit the activity of the copper amine oxidase from arthrobacter globiformis (agao), with k(i) values between 6 mum and 37 nm. in the crystal structure of a ru(ii)-wire:agao conjugate, the wire occupies the agao active-site substrate access channel, the trihydroxyphenylalanine quinone cofactor is ordered in the "off-cu" position with its reactive carbonyl oriented toward th ...200516157884
x-ray crystallographic analysis of 6-aminohexanoate-dimer hydrolase: molecular basis for the birth of a nylon oligomer-degrading enzyme.6-aminohexanoate-dimer hydrolase (eii), responsible for the degradation of nylon-6 industry by-products, and its analogous enzyme (eii') that has only approximately 0.5% of the specific activity toward the 6-aminohexanoate-linear dimer, are encoded on plasmid poad2 of arthrobacter sp. (formerly flavobacterium sp.) ki72. here, we report the three-dimensional structure of hyb-24 (a hybrid between the eii and eii' proteins; eii'-level activity) by x-ray crystallography at 1.8 a resolution and refin ...200516162506
involvement of the c-terminal tail of arthrobacter ureafaciens sialidase isoenzyme m in cleavage of the internal sialic acid of ganglioside gm1.arthrobacter ureafaciens sialidase comprises four isoenzymes, l, m1, m2 and s, of which l, m1, and m2, but not s, have the unique ability to cleave gm1 ganglioside, but the hydrolysis of gm3 and colominic acid by s occurs at a higher rate than that by l, m1 and m2. since the n-terminal amino acid sequences of l, m1, m2 and s were shown to be identical on protein sequencing, they were suggested to have arisen from the same protein through truncation at different c-terminal sites. a dna segment co ...200516169883
microbial production of optically active beta-phenylalanine ethyl ester through stereoselective hydrolysis of racemic beta-phenylalanine ethyl ester.the ability to produce (r)- or (s)-beta-phenylalanine ethyl ester (3-amino-3-phenylpropionic acid ethyl ester, bpae) from racemic bpae through stereoselective hydrolysis was screened for in bpae-assimilating microorganisms. sphingobacterium sp. 238c5 and arthrobacter sp. 219d2 were found to be potential catalysts for (r)- and (s)-bpae production, respectively. on a 24-h reaction, with 2.5% (w/v) racemic bpae (130 mm) as the substrate and wet cells of sphingobacterium sp. 238c5 as the catalyst, 1 ...200616170530
cold-active beta-galactosidase from arthrobacter sp. c2-2 forms compact 660 kda hexamers: crystal structure at 1.9a resolution.the x-ray structure of cold-active beta-galactosidase (isoenzyme c-2-2-1) from an antarctic bacterium arthrobacter sp. c2-2 was solved at 1.9a resolution. the enzyme forms 660 kda hexamers with active sites opened to the central cavity of the hexamer and connected by eight channels with exterior solvent. to our best knowledge, this is the first cold-active beta-galactosidase with known structure and also the first known beta-galactosidase structure in the form of compact hexamers. the hexamer or ...200516171818
monitoring arthrobacter protophormiae rkj100 in a 'tag and chase' method during p-nitrophenol bio-remediation in soil microcosms.monitoring of micro-organisms released deliberately into the environment is essential to assess their movement during the bio-remediation process. during the last few years, dna-based genetic methods have emerged as the preferred method for such monitoring; however, their use is restricted in cases where organisms used for bio-remediation are not well characterized or where the public domain databases do not provide sufficient information regarding their sequence. for monitoring of such micro-or ...200616205921
the role of histidine 200 in mndd, the mn(ii)-dependent 3,4-dihydroxyphenylacetate 2,3-dioxygenase from arthrobacter globiformis cm-2, a site-directed mutagenesis study.the manganese-dependent 3,4-dihydroxyphenylacetate 2,3-dioxygenase (mndd) from arthrobacter globiformis cm-2 is an extradiol-cleaving catechol dioxygenase that catalyzes aromatic ring cleavage of 3,4-dihydroxyphenylacetate (dhpa). based on the recent crystal structure of the mndd-dhpa complex, a series of site-directed mutations were made at a conserved second-sphere residue, histidine 200, to gain insight into and clarify the role this residue plays in the mn(ii)-dependent catalytic mechanism. ...200516217642
chemo-enzymatic synthesis of a calcitonin derivative containing a high-mannose type oligosaccharide by endo-beta-n-acetylglucosaminidase from arthrobacter protophormiae.chemo-enzymatic addition of a high-mannose type oligosaccharide to eel calcitonin (ct), a calcium-regulating hormone, was examined. the endo-beta-n-acetylglucosaminidase from arthrobacter protophormiae (endo-a) transglycosylated the man(6)-glcnac moiety from an ovalbumin-derived high-mannose type glycosyl asparagine, asn(man(6)-glcnac(2))-oh, to a calcitonin derivative, [asn(glcnac)(3)]-ct, in which the n-acetyl-d-glucosamine (glcnac) is attached to the third l-asparagine (asn) residue of the pe ...199916232446
molecular cloning and nucleotide sequencing of organophosphorus insecticide hydrolase gene from arthrobacter sp. strain b-5.the organophosphorus insecticide hydrolase (oph) gene of arthrobacter sp. strain b-5, isolated from turf green soil was cloned into escherichia coli jm109. three clones, termed epb511, epb521 and epb531, exhibiting oph activity were obtained. however, these three clones showed lower op-degrading ability than strain b-5. a 7.7-kb inserted fragment of the plasmid pb521 harbored by epb521 was subcloned, resulting in construction of a plasmid, pb526, carrying the 2.6-kb inserted fragment with op-deg ...199916232510
isolation and characterization of psychrotrophs from subterranean environments.subterranean environments are potential sources for the isolation of novel microorganisms. water and soil samples were collected at depths ranging from 10 to 1800 meters below the surface, and screening was carried out with aerobic rich and anaerobic minimal media. two psychrotrophic and three chemoautotrophic strains were isolated. one of the psychrotrophic isolates, designated sn16a, grew at temperatures between -5 and 37 degrees c with optimal growth between 25 and 30 degrees c. the other psy ...199916232548
cloning of inulin fructotransferase (dfa iii-producing) gene from arthrobacter globiformis c11-1.a gene encoding an inulin fructotransferase (dfa iii-producing) [ec] from arthrobacter globiformis c11-1 was cloned and the nucleotide sequence was determined. the cloned fragment contained a 1353 bp open reading frame. the initiation codon was estimated to be an unusual codon, gtg. the gene encoded a signal peptide (40 amino acid residues) for secretion. the molecular mass of the native enzyme was calculated as 43,400 da from the sequencing data. the deduced amino acid sequence of the ...200016232803
plate assay for endo-beta-n-acetylglucosaminidase activity using a chromogenic substrate synthesized by transglycosylation with arthrobacter endo-beta-n-acetylglucosaminidase.the transglycosylation activity of arthrobacter endo-beta-n-acetylglucosaminidase (endo-a) was used for the enzymatic synthesis of a novel oligosaccharide, man6glcnac-5-bromo-4-chloro-3-indolyl-beta-glucoside (man6glcnac-glc-beta-x). various endo-beta-n-acetylglucosaminidases hydrolyzed this oligosaccharide, producing man6glcnac and glc-beta-x. the e. coli strains coexpressing endo-a and beta-glucosidase formed blue colonies in the presence of man6glcnac-glc-beta-x. therefore, endo-beta-n-acetyl ...200016232892
purification and properties of betaine aldehyde dehydrogenase with high affinity for nadp from arthrobacter globiformis.betaine aldehyde dehydrogenase from arthrobacter globiformis was purified to apparent homogeneity by ammonium sulfate fractionation, followed by ion-exchange, butyl-toyopearl and gel filtration chromatography. the enzyme was found to be a tetramer with identical 55 kda subunits. both nad+ and nadp+ could be used as a cofactor for the enzyme and michaelis constants (k(m) value) for nad+ and nadp+ were 1075 microm and 48 microm, respectively. the enzyme was highly specific for betaine aldehyde and ...200216233177
transfer of high-mannose-type oligosaccharides to disaccharides by endo-beta-n-acetylglucosaminidase from arthrobacter protophormiae.endo-beta-n-acetylglucosaminidase from arthrobacter protophormiae (endo-a) has transglycosylation activity, and high-mannose-type oligosaccharides are transferred to suitable glycosides as acceptor substrates. the acceptor specificity of endo-a-catalyzed transglycosylation toward various disaccharides was investigated. to identify an effective acceptor for the transglycosylation by endo-a, the reaction was carried out using various disaccharides. endo-a transferred high-mannose-type oligosacchar ...200216233259
removal and recovery of uranyl ion using various microorganisms.the adsorption of uranyl ion by microorganisms was examined. among the 76 strains of 69 species tested (23 bacteria, 20 actinomycetes, 18 fungi, and 15 yeasts), high uranyl ion adsorption ability was exhibited by strains of the bacteria, arthrobacter nicotianae, bacillus subtilis, and micrococcus luteus. a. nicotianae cells, which showed the best performance, could adsorb about 698 mg uranyl ion (2.58 mmol) per gram dry wt. of microbial cells. the adsorption of uranyl ion was rapid, selective, a ...200216233264
molecular cloning of the gene encoding the di-d-fructofuranose 1,2':2,3' dianhydride hydrolysis enzyme (dfa iiiase) from arthrobacter sp. h65-7.the gene encoding an intracellular enzyme hydrolyzing di-d-fructofuranose 1,2':2,3' dianhydride (dfa iii) (dfa iiiase) was cloned from the genomic dna of arthrobacter sp. h65-7 for the first time. the single open reading frame (orf) of the dfa iiiase gene consisted of 1368-bp encoding 455 amino acids. dfa iiiase showed a phylogenetically distinct position from other inulin-degrading enzymes and showed similarity only with inulin fructotransferases (depolymerizing) (inulase ii, ec from ...200316233453
a thermostable histamine oxidase from arthrobacter crystallopoietes kait-b-007.a thermostable histamine oxidase (ec 1.4.3.-) was found in cells of arthrobacter crystallopoietes kait-b-007 isolated from soil. the enzyme was purified about 715-fold over the cell free extracts with a yield of 55% by ammonium sulfate fractionation and various column chromatographies. the purified enzyme was homogeneous on polyacrylamide gel-electrophoresis (native-page). when the enzyme was kept at 65 degrees c and 70 degrees c for 10 min, the activity was fully stable at 65 degrees c and decr ...200416233600
cloning and heterologous expression of a glucodextranase gene from arthrobacter globiformis i42, and experimental evidence for the catalytic diad of the recombinant enzyme.the gene encoding a glucodextranase from arthrobacter globiformis i42 was cloned and, subsequently, heterologously expressed in escherichia coli. this glucodextranase gene consists of 1048 amino acid residues with a calculated molecular mass of 109,135 da. the roles of two residues at the active site of a. globiformis i42 glucodextranase were examined by site-directed mutagenesis. glutamic acid residues 458 and 656, which are part of the apparent catalytic residues, were found to be essential fo ...200416233603
cloning and heterologous expression of a beta-fructofuranosidase gene from arthrobacter globiformis ifo 3062, and site-directed mutagenesis of the essential aspartic acid and glutamic acid of the active site.we have cloned the gene encoding a beta-fructofuranosidase from arthrobacter globiformis ifo 3062, and subsequently, the gene was heterologously expressed in escherichia coli. this beta-fructofuranosidase gene encodes a protein of 548 amino acid residues with a calculated molecular mass of 60,519 da. we have examined the roles of three residues of a. globiformis ifo 3062 beta-fructofuranosidase by site-directed mutagenesis, and found that aspartic acid 130 and glutamic acid 392, which are two of ...200416233623
molecular cloning and expression of uricase gene from arthrobacter globiformis in escherichia coli and characterization of the gene product.arthrobacter globiformis ferm bp-360 produces uricase (urate oxidase; ec intracellularly. a genomic library of the bacterium, prepared in the plasmid vector puc118, was screened with probes based on the amino acid sequence of the purified uricase. we found that a chimeric plasmid in the library, designated puod1, carries a 2.0-kb dna insert from the arthrobacter dna that hybridizes with the probe. the dna insert contains an orf consisting of 302 amino acids with a calculated molecular m ...200416233683
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