[attempt at identification of 2 strains of arthrobacter by study of their antigenic properties and their resistance to antibiotics]. 196314048720
[research on various species of nitrogen-fixing arthrobacter isolated from karstic alpine rocks]. 196314048724
the assimilation of 2-c compounds other than acetate. 196314053263
new bacterial polysaccharide from arthrobacter.a bacterial strain (nrrl b-1973) isolated from soil at guatemala city and tentatively identified as an arthrobacter species produced a polysaccharide with unusual properties. conditions were studied for the production of this microbial gum in shaken flasks and 20-liter fermentors. suitable nutrients for optimal polysaccharide production included 3% glucose, 0.3% enzyme-hydrolyzed casein, magnesium sulfate, manganese sulfate, and potassium phosphate buffer (ph 7.0). polysaccharide yields ranged f ...196314075048
a crystalline pigment produced from 2-hydroxypyridine by arthrobacter crystallopoietes n.sp. 196314106078
morphological aberration of arthrobacter globiformis cells due to biotin deficiency.chan, e. c. s. (university of new brunswick, fredericton, new brunswick, canada). morphological aberration of arthrobacter globiformis cells due to biotin deficiency. j. bacteriol. 87:641-651. 1964.-morphological aberration of arthrobacter globiformis strain 425 was shown to occur during growth in a chemically defined medium without added biotin. such aberrant cells could revert back to normal coccoid forms upon inoculation into fresh medium supplemented with the vitamin. this abnormal cellular ...196414127583
nutritional control of morphogenesis in arthrobacter crystallopietes.ensign, jerald c. (university of illinois, urbana), and r. s. wolfe. nutritional control of morphogenesis in arthrobacter crystallopoietes. j. bacteriol. 87:924-932. 1964.-arthrobacter crystallopoietes exhibits the cyclic, morphological variation which is a characteristic of this genus. a simple chemically defined medium was developed in which this organism is restricted to growth and division entirely in the coccoid form. addition singly to this medium of l-arginine, l-phenylalanine, l-asparagi ...196414137632
branching enzyme of arthrobacter globiformis. 196414171890
an antibiotic produced by the mycorrhizal fungus cenococcum graniforme. 196414187005
use of arthrobacter terregens for bioassay of mycobactin.reich, claude v. (johns hopkins-leonard wood memorial leprosy research laboratory, johns hopkins university, baltimore, md.), and john h. hanks. use of arthrobacter terregens for bioassay of mycobactin. j. bacteriol. 87:1317-1320. 1964.-arthrobacter terregens was used to assay mycobactin, a growth factor for mycobacterium paratuberculosis. within 7 days, a. terregens gave a linear photometric growth response to mycobactin in the range of 0.05 to 0.2 mug/ml. preparations found to be active (or in ...196414188708
metabolism of coumarin by a micro-organism. 196414195065
adenosine diphosphate glucose-glycogen transglucosylase in arthrobacter sp. nrrl b 1973. 196414205498
metabolism of ethanolamine. an ethanolamine oxidase. 196414209947
specificity of improved methods for mycobactin bioassay by arthrobacter terregens.antoine, alan d. (johns hopkins university-leonard wood memorial leprosy research laboratory, baltimore, md.), norman e. morrison, and john h. hanks. specificity of improved methods for mycobactin bioassay by arthrobacter terregens. j. bacteriol. 88:1672-1677. 1964.-arthrobacter terregens was used to assay mycobactin, a growth factor for mycobacterium paratuberculosis. improved techniques permit the assay of mycobactin within 3 to 4 days by agarplate or liquid-medium methods. for the agarplate m ...196414240956
metabolism of coumarin by a micro-organism: o-coumaric acid as an intermediate between coumarin and melilotic acid. 196414243380
some studies on trichloroacetate-decomposing soil bacteria. 196414245149
sugar nucleotide reactions in arthrobacter. i. guanosine diphosphate mannose pyrophosphorylase: purification and properties. 196414245350
sugar nucleotide reactions in arthrobacter. ii. biosynthesis of guanosine diphosphomannuronate. 196414245351
the occurrence of adenosine diphosphate glucose: glycogen transglucosylase in bacteria. 196414247687
the metabolism of coumarin by a microorganism. ii. the reduction of o-coumaric acid to melilotic acid. 196414269315
microbial degradation of 2,2-dichloropropionic acid in five soils. 196414272481
the activation of escherichia coli adp-glucose pyrophosphorylase. 196514282015
studies on the biochemical basis of the minimum temperatures for growth of certain psychrophilic and mesophilic micro-organisms. 196514283028
synthetic metal chelators which replace the natural growth-factor requirements of arthrobacter terregens. 196514291610
biosynthesis of bacterial glycogen. i. purification and properties of the adenosine diphosphoglucose pyrophosphorylase of arthrobacter species nrrl b1973. 196514304834
biosynthesis of bacterial glycogen. ii. purification and properties of the adenosine diphosphoglucose:glycogen transglucosylase of arthrobacter species nrrl b1973. 196514304835
fate of bacteria in chicken meat during freeze-dehydration, rehydration, and plate counts were determined on boneless cooked, cubed chicken meat obtained from a commercial processor. survival of the natural flora was determined after the meat was freeze-dehydrated and rehydrated at room temperature for 30 min and 50, 85, and 100 c for 10 min. total counts of bacteria in the rehydrated samples were determined during storage of the meat at 4, 22, and 37 c until spoilage odor was detectable. meat samples were inoculated with staphylococcus aureus, then dried, rehydrat ...196514325271
microbial degradation of isopropyl-n-3 -chlorophenylcarbamate and 2-chloroethyl-n-3-chlorophenylcarbamate.microbial degradation of isopropyl-n-3-chlorophenylcarbamate (cipc) and 2-chloroethyl-n-3-chlorophenylcarbamate (cepc) was observed in a soil perfusion system. degradation in perfused soils, and by pure cultures of effective bacterial isolates, was demonstrated by the production of 3-chloroaniline and the subsequent liberation of free chloride ion. identified isolates effective in degrading and utilizing cipc as a sole source of carbon included pseudomonas striata chester, a flavobacterium sp., ...196514325285
lysis of bacterial cell walls by an enzyme isolated from a myxobacter.ensign, j. c. (university of illinois, urbana), and r. s. wolfe. lysis of bacterial cell walls by an enzyme isolated from a myxobacter. j. bacteriol. 90:395-402. 1965.-an exoenzyme which lyses intact cells, heat-killed cells, and cell walls of arthrobacter crystallopoietes was purified 60-fold from the growth liquor obtained from a myxobacter (strain al-1). the lytic enzyme was produced during growth of the organism in a number of complex media, the maximal amount of enzyme being produced in yea ...196514330733
studies of the lipids of arthrobacter globiformis 616. i. the fatty acid composition. 196514332529
microorganisms in the root zone in relation to soil moisture. 196514346123
indigoidine and other bacterial pigments related to 3,3'-bipyridyl. 196514347925
auxin production by species of arthrobacter. 196114454424
[isolation and purification of aerobic cellulolytic soil bacteria. ii. isolation and description of a new arthrobacter species associated with sporocytophaga myxococcoides]. 196114492583
wheaton rb: continuity of psychrophilic and mesophilic growth characteristics in the genus arthrobacter.roth, norman g. (whirlpool corp., st. joseph, mich.) and robert b. wheaton. continuity of psychrophilic and mesophilic growth characteristics in the genus arthrobacter. j. bacteriol. 83:551-555. 1962.-the effect of temperature on growth of seven members of the genus arthrobacter was determined at 0, 7, 20, 30, and 37 c. in general, no sharp cutoff point was observed between growth-temperature requirements of psychrophilic and mesophilic bacteria. there appeared to be a continuous gradation among ...196214494403
biotransformations of propenylbenzenes by an arthrobacter sp. and its t-anethole blocked mutants.propenylbenzenes are often used as starting materials in the chemical synthesis of aroma compounds and fine chemicals. in the present study, we demonstrate the ability of an arthrobacter sp. to transform various structures of propenylbenzenes derived from essential oils to flavor, fragrance, and fine chemicals. arthrobacter strain ta13 and its t-anethole blocked mutants (incapable of growing on t-anethole) converted isoeugenol to vanillin and vanillic acid; and safrole to hydroxychavicol. high c ...200314511910
pyrocoll, an antibiotic, antiparasitic and antitumor compound produced by a novel alkaliphilic streptomyces strain.a new secondary metabolite was detected in the culture extract of streptomyces sp. ak 409 by hplc-diode-array screening. the metabolite was identified as pyrocoll, which is known to be a constituent of cigarette smoke. pyrocoll is known as a synthetic compound, but until now had not been isolated as a natural product from a microorganism. the compound showed biological activity against various arthrobacter strains, filamentous fungi, several pathogenic protozoa, and some human tumor cell lines.200314513907
[the quantity and structure of the root-associated microbial complexes of two greenhouse rose cultivars].the study of the root-associated microbial complexes of affected and healthy rose plants of two cultivars (grand gala and royal velvet) grown in a greenhouse showed that the biomass of eukaryotic microorganisms in the rhizoplane and rhizosphere of healthy rose plants and in the surrounding soil was considerably lower than in the same loci of affected plants. in contrast, the biomass of root-associated prokaryotic microorganisms was higher in the case of healthy than in the case of affected rose ...200314526548
identification and differentiation of species and strains of arthrobacter and microbacterium barkeri isolated from smear cheeses with amplified ribosmal dna restriction analysis (ardra) and pulsed field gel electrophoresis (pfge).ardra (amplified ribosomal-dna restriction analysis) was used to differentiate among species and genera of arthrobacter and microbacteria. species-specific restriction patterns of pcr-products were obtained with ncii for arthrobacter citreus (dsm 20133t), a. sulfureus (dsm 20167t), a. globiformis (dsm 20124t) and a. nicotianae strains (dsm 20123t, mge 10d, ca13, ca14, isolate 95293, 95294, and 95299), a. rhombi ccug 38813t, and ccug 38812, and microbacterium barkeri strains (dsm 30123t, mge 10d, ...200314529187
characterization of hdnor, the transcriptional repressor of the 6-hydroxy-d-nicotine oxidase gene of arthrobacter nicotinovorans pao1, and its dna-binding activity in response to l- and d-nicotine derivatives.utilization of l-nicotine as growth substrate by arthrobacter nicotinovorans pao1 starts with hydroxylation of the pyridine ring at c6. next, the pyrrolidine ring is oxidized by 6-hydroxy-l-nicotine oxidase, which acts strictly stereo-specific on the l-enantiomer. surprisingly, l-nicotine also induces the synthesis of a 6-hydroxy-d-nicotine-specific oxidase in the bacteria. genes of nicotine-degrading enzymes are located on the catabolic plasmid pao1. the pao1 sequence revealed that the 6-hydrox ...200314534317
influence of al on growth, cell size and content of intracellular water of arthrobacter sp. pi/1-95.a distinct inhibition of the growth of a typical soil-born bacterium, arthrobacter sp. pi/1-95, by aluminium was established. the content of intracellular water and (consequently) the cell size of the bacterium significantly increased when cells came into contact with aluminium. both parameters were also increased when applying hcl, but al caused the greater change, so that a distinct discrimination between the effects of aluminium on the one hand and a sole ph-effect on the other hand was possi ...200314574119
electron spin resonance study of chromium(v) formation and decomposition by basalt-inhabiting bacteria.bacterial reduction of cr(vi) to cr(iii) compounds may produce reactive intermediates cr(v) and cr(iv), which can affect the mobility and toxicity of chromium in environments. to address this important subject, we conducted an electron spin resonance (esr) study to understand the kinetics of the formation and decomposition of cr(v) during cr(vi) reduction by different gram-positive cr(vi)-tolerant bacteria, which were isolated from polluted basalts from the united states of america and the repub ...200314594378
production of an egg yolk antibody specific to microbial uricase and its inhibitory effects on uricase activity.ammonia gas produced from poultry manure can be a potential source of environmental pollution. microbial uricase in poultry manure is an important target enzyme to reduce ammonia production because ammonia is mainly generated from the microbial decomposition of uric acid in the manure. thus, the inhibition of microbial uricase is critical in preventing nh3 volatilization. a potential method of inhibiting uricase activity is the use of antibodies specific to microbial uricase. therefore, this stu ...200314601732
diversity of l-leucine catabolism in various microorganisms involved in dairy fermentations, and identification of the rate-controlling step in the formation of the potent flavour component 3-methylbutanal.various microorganisms, belonging to the genera lactococcus, lactobacillus, streptococcus, leuconostoc, bifidobacterium, propionibacterium, brevibacterium, corynebacterium and arthrobacter, used in dairy fermentations such as cheese making, were analysed for their potential to convert leucine into flavour components, most notably 3-methylbutanal. a large variation between and within species was observed for various enzyme activities involved in the conversion pathway, e.g. transaminases, alpha-h ...200414624315
expression, crystallization and preliminary x-ray crystallographic studies of arthrobacter globiformis inulin fructotransferase.a recombinant form of arthrobacter globiformis inulin fructotransferase (dfaiii-producing) has been overexpressed in escherichia coli and purified to homogeneity. crystals were obtained at 293 k by the hanging-drop vapour-diffusion technique using 0.1 m na hepes ph 7.5 buffer containing 1.5 m lithium sulfate as a precipitant. crystals of the recombinant wild-type enzyme diffracted to better than 1.5 a at 100 k using a synchrotron-radiation source at the photon factory. the crystal belonged to sp ...200314646096
arthrobacter gandavensis sp. nov., for strains of veterinary origin.three strains of a previously undescribed, gram-positive, coryneform bacterium, which were isolated from cattle, were subjected to polyphasic taxonomic analysis. comparative 16s rrna gene sequencing revealed that the unknown isolates were members of the genus arthrobacter and were phylogenetically closely related to arthrobacter luteolus. however, dna-dna hybridization indicated that the strains belonged to a new sub-lineage within the genus arthrobacter. the unknown isolates can be distinguishe ...200314657117
structural insights into substrate specificity and function of glucodextranase.a glucodextranase (igdase) from arthrobacter globiformis i42 hydrolyzes alpha-1,6-glucosidic linkages of dextran from the non-reducing end to produce beta-d-glucose via an inverting reaction mechanism and classified into the glycoside hydrolase family 15 (gh15). here we cloned the igdase gene and determined the crystal structures of igdase of the unliganded form and the complex with acarbose at 2.42-a resolution. the structure of igdase is composed of four domains n, a, b, and c. domain a forms ...200414660574
reconfirmation of antimicrobial activity in the coelomic fluid of the earthworm eisenia fetida andrei by colorimetric assay.a novel tetrazolium salt, 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulphophenyl)-2h-tetrazolium (mts) was used in the assessment of antimicrobial activity in earthworm in the presence of phenazine methosulphate (pms) as an electron coupling reagent. this activity was purified from the coelomic fluid of the earthworm (ecf), eisenia fetida andrei (oligochaeta, lumbricidae, annelids) using a series of column chromatography techniques and was tested against three gram-negative st ...200314660872
bacterial composition of the biofilm on the surface of course sediment of the danube: with special reference to the clinically important bacteria.on monthly intervals and over a period of 14 months (november, 1993-december, 1994) biofilm samples from sediments taken at the szentendre island on the danube were culturel and the isolated organisms were examined macromorphologically and micromorphogically and tested for oxidase and catalase production and their ability to oxidise and ferment glucose. the majority (85%) of the strains isolated were catalase positive. 43% were oxidase positive, 38% were glucose oxidisers and only 19% fermented ...199814666747
microbial synthesis of (r)- and (s)-3,4-dimethoxyamphetamines through stereoselective transamination.two soil isolates, arthrobactersp. knk168 and pseudomonas sp. knk425, aminated 3,4-dimethoxyphenylacetone in presence of sec-butylamine as an amino donor to yield 3,4-dimethoxyamphetamine (dma) with different enantioselectivities. the former gave (r)-dma (>99% e.e.) and the latter the (s)-isomer (>99% e.e.).200314677709
cloning, sequence analysis, and purification of choline oxidase from arthrobacter globiformis: a bacterial enzyme involved in osmotic stress tolerance.choline oxidase catalyzes the four-electron oxidation of choline to glycine betaine, one of a limited number of compounds that accumulate to high levels in the cytoplasm of cells to prevent dehydration and plasmolysis in adverse hyperosmotic environments. in the present study, the highly gc rich coda gene encoding for choline oxidase was cloned from genomic dna of arthrobacter globiformis strain atcc 8010 and expressed to high yields in escherichia coli strain rosetta(de3)plyss. the resulting en ...200414678796
branching of o-nitrobenzoate degradation pathway in arthrobacter protophormiae rkj100: identification of new intermediates.we have earlier reported a novel reductive pathway for o-nitrobenzoate (onb) degradation (at 0.5 mm) in arthrobacter protophormiae rkj100, which proceeds via the formation of o-hydroxylaminobenzoate (haba) and anthranilate (aa). during growth of this organism at 40 times higher concentration (20 mm) of onb, 3-hydroxyanthranilate (haa) was identified as an intermediate by thin layer chromatography, gas chromatography and high performance liquid chromatography studies. crude cell extracts of onb-g ...200314680704
the crystal structure of pichia pastoris lysyl oxidase.pichia pastoris lysyl oxidase (pplo) is unique among the structurally characterized copper amine oxidases in being able to oxidize the side chain of lysine residues in polypeptides. remarkably, the yeast pplo is nearly as effective in oxidizing a mammalian tropoelastin substrate as is a true mammalian lysyl oxidase isolated from bovine aorta. thus, pplo is functionally related to the copper-containing lysyl oxidases despite the lack of any significant sequence similarity with these enzymes. the ...200314690425
spectroscopic and kinetic properties of recombinant choline oxidase from arthrobacter globiformis.choline oxidase catalyzes the four-electron oxidation of choline to glycine betaine, with molecular oxygen acting as primary electron acceptor. recently, the recombinant enzyme expressed in escherichia coli was purified to homogeneity and shown to contain fad in a mixture of oxidized and anionic semiquinone redox states [fan et al. (2003) arch. biochem. biophys., in press]. in this study, methods have been devised to convert the enzyme-bound flavin semiquinone to oxidized fad and vice versa, all ...200314690428
a simple method for differential isolation of freely dispersed and particle-associated peat microorganisms.differential isolation of physiologically and ecologically diverse microbial groups facilitates evaluation of their activities in the environment. here we describe a new method for differential isolation of freely dispersed and particle-associated peat microorganisms. the method is based on sequential elution of peat. two types of natural peat, "young" sphagnum peat and "old" reed-based peat that have significantly different molecular composition as revealed by solid-state 13c nmr, were used for ...200414706754
stereochemistry of 2-phenylethylamine oxidation catalyzed by bacterial copper amine oxidase.the stereochemical course of the reaction catalyzed by a copper amine oxidase from arthrobacter globiformis has been investigated using 2-phenylethylamine stereospecifically deuterium-labeled at the c1 position. measurements of deuterium content in the product, phenylacetaldehyde, by gas chromatography-mass spectrometry revealed stereospecific abstraction of the pro-s hydrogen during the enzymatic oxidation, as predicted from the structure modeling for the enzyme-bound substrate.200314730151
bacterial populations associated with the oxidation and reduction of arsenic in an unsaturated soil.microbial populations responsible for the oxidation and reduction of as were examined in unsaturated (aerobic) soil columns treated with 75 microm arsenite [as(iii)] or 250 microm arsenate [as(v)]. arsenite [as(iii)] was rapidly oxidized to as(v) via microbial activity, whereas no apparent reduction of as(v) was observed in the column experiments. eight aerobic heterotrophic bacteria with varying as redox phenotypes were isolated from the same columns. three isolates, identified as agrobacterium ...200414740724
reconsideration of arthrobacter ilicis (mandel et al. 1961) collins et al. 1982 as a plant-pathogenic species. proposal to emend the authority and description of the species. request for an opinion.strains now considered to represent the type strain of arthrobacter ilicis, described as a pathogen of american holly, are not identical. the designated type strain does not represent this pathogen. however, one of the other strains sourced to the type strain of the pathogen does appear to be authentic, but is not a member of a. ilicis. it is proposed that a. ilicis is an unrelated species, not a pathogen of american holly. the nomenclature of a. ilicis can be rectified by emending the authority ...200414742500
dna isolation from soil samples for cloning in different hosts.many protocols to extract dna directly from soil samples have been developed in recent years. we employed two extraction methods which differed in the method of lysis and compared these methods with respect to yield, purity and degree of shearing. the main focus was on the specific isolation of dna from different microorganisms, especially dna from actinomycetes, as these cells are very difficult to lyse, in contrast to non-actinomycetes. thus, we used both methods to isolate dna from pseudomona ...200414758515
arthrobacter woluwensis subacute infective endocarditis: case report and review of the literature.we report a case of endocarditis due to arthrobacter woluwensis and review the published reports of arthrobacter species isolated from human clinical samples. a 39-year-old injection drug user presented with fever and a new heart murmur. a. woluwensis was isolated from blood cultures, and a diagnosis of subacute infective endocarditis of the native mitral valve was made. the patient was successfully treated with a 6-week course of intravenous teicoplanin. from our review of the literature, we we ...200414765360
[a new aerobic gram-positive bacterium with a unique ability to degrade ortho- and para-chlorinated biphenyls].strain b51 capable of degrading polychlorinated biphenyls (pcb) was isolated from soil contaminated with wastes from the chemical industry. based on its morphological and chemotaxonomic characteristics, the strain was identified as a microbacterium sp. experiments with washed cells showed that strain b51 is able to degrade ortho- and para-substituted mono-, di-, and trichlorinated biphenyls (mcb, dcb, and tcb, respectively). unlike the known pcb degraders, microbacterium sp. b51 is able to oxidi ...200314768541
purification, characterization, and gene cloning of cis,cis-muconate cycloisomerase from benzamide-assimilating arthrobacter sp. ba-5-17.cis,cis-muconate cycloisomerase (mc) was purified to homogeneity from benzamide-assimilating arthrobacter sp. ba-5-17. the purified enzyme showed high activities for cis,cis-muconate and 3-methyl-cis,cis-muconate, and preferred the 3-substituted derivatives over the derivatives with the same substituent at the 2 position as a substrate. a gene encoding mc of strain ba-5-17 was cloned and named catb. the catb gene was clustered with catr encoding a putative lysr-type regulator, catc encoding a pu ...200414769475
some factors influencing enzymic activities of corynebacterium creatinovorans. 195014781175
the mechanism of acetate oxidation by corynebacterium creatinovorans. 195014790801
degradation of phenol and toxicity of phenolic compounds: a comparison of cold-tolerant arthrobacter sp. and mesophilic pseudomonas putida.phenol degradation efficiency of cold-tolerant arthrobacter sp. ag31 and mesophilic pseudomonas putida dsm6414 was compared. the cold-tolerant strain was cultivated at 10 degrees c, while the mesophile was grown at 25 degrees c. both strains degraded 200 mg and 400 mg phenol/l within 48-72 h of cultivation, but the cold-tolerant strain produced more biomass than the mesophile. both strains oxidized catechol by the ortho type of ring fission. catechol 1,2 dioxygenase (c1,2d) activity was found in ...200414872323
chemical rescue of a site-specific mutant of bacterial copper amine oxidase for generation of the topa quinone cofactor.the topa quinone (tpq) cofactor of copper amine oxidase is produced by posttranslational modification of a specific tyrosine residue through the copper-dependent, self-catalytic process. we have site-specifically mutated three histidine residues (his431, his433, and his592) involved in binding of the copper ion in the recombinant phenylethylamine oxidase from arthrobacter globiformis. the mutant enzymes, in which each histidine was replaced by alanine, were purified in the cu/tpq-free precursor ...200414979714
hexavalent chromium reduction by an actinomycete, arthrobacter crystallopoietes es 32.environmental contamination by hexavalent chromium, cr(vi), presents a serious public health problem. this study assessed the reduction of cr(vi) by intact cells and a cell-free extract (cfe) of an actinomycete, arthrobacter crystallopoietes (strain es 32), isolated from soil contaminated with dichromate. both intact cells and cfe of a. crystallopoietes, displayed substantial reduction of cr(vi). intact cells reduced about 90% of the cr(vi) added within 12 h and cr(vi) was almost completely redu ...200414985627
cyanide as a copper and quinone-directed inhibitor of amine oxidases from pea seedlings ( pisum sativum) and arthrobacter globiformis: evidence for both copper coordination and cyanohydrin derivatization of the quinone cofactor.the interactions of cyanide with two copper-containing amine oxidases (cuaos) from pea seedlings (psao) and the soil bacterium arthrobacter globiformis (agao) have been investigated by spectroscopic and kinetic techniques. previously, we rationalized the effects of azide and cyanide for several cuaos in terms of copper coordination by these exogenous ligands and their effects on the internal redox equilibrium tpq(amr)-cu(ii) right harpoon over left harpoon tpq(sq)-cu(i). the mechanism of cyanide ...200414986071
whole cell-enzyme hybrid amperometric biosensor for direct determination of organophosphorous nerve agents with p-nitrophenyl this paper, we reported the construction of a hybrid biosensor for direct, highly selective, sensitive, and rapid quantitative determination of organophosphate pesticides with p-nitrophenyl substituent using purified organophosphorus hydrolase (oph) for the initial hydrolysis and arthrobacter sp. js443 for subsequent p-nitrophenol oxidation. the biocatalytic layer was prepared by co-immobilizing arthrobacter sp. js443 and oph on a carbon paste electrode. oph catalyzed the hydrolysis of organo ...200414991648
crystallization and preliminary x-ray study of isomaltodextranase from arthrobacter globiformis.a recombinant isomaltodextranase (1,6-alpha-d-glucan isomaltohydrolase; ec from an arthrobacter sp. that hydrolyzes dextrans to generate isomaltose was purified and crystallized using the sitting-drop vapour-diffusion method at 293 k. x-ray diffraction data were collected to 1.8 a. the crystals belong to space group c2, with unit-cell parameters a = 199.1, b = 62.7, c = 57.4, beta = 101.4 degrees. analysis of the patterson self-rotation function suggests that the crystal contains one p ...200414993697
high-resolution crystal structure of arthrobacter aurescens chondroitin ac lyase: an enzyme-substrate complex defines the catalytic mechanism.chondroitin lyases (ec and ec are glycosaminoglycan-degrading enzymes that act as eliminases. chondroitin lyase ac from arthrobacter aurescens (arthroac) is known to act on chondroitin 4-sulfate and chondroitin 6-sulfate but not on dermatan sulfate. like other chondroitin ac lyases, it is capable of cleaving hyaluronan. we have determined the three-dimensional crystal structure of arthroac in its native form as well as in complex with its substrates (chondroitin 4-sulfate tetras ...200415003453
reclassification of brevibacterium liquefaciens okabayashi and masuo 1960 as arthrobacter nicotianae giovannozzi-sermanni 1959.brevibacterium liquefaciens atcc 14929(t) was reclassified as corynebacterium liquefaciens by lanéelle et al. (1980). a further study by stackebrandt et al. (1983) described the same strain, indicating high genomic and chemotaxonomic relatedness to arthrobacter nicotianae; however, reclassification was not formally proposed. because of the discrepancies between their previous work and the data of collins & kroppenstedt (1983), lanéelle and colleagues re-examined b. liquefaciens and withdrew thei ...200415023984
crystallographic comparison of manganese- and iron-dependent homoprotocatechuate 2,3-dioxygenases.the x-ray crystal structures of homoprotocatechuate 2,3-dioxygenases isolated from arthrobacter globiformis and brevibacterium fuscum have been determined to high resolution. these enzymes exhibit 83% sequence identity, yet their activities depend on different transition metals, mn2+ and fe2+, respectively. the structures allow the origins of metal ion selectivity and aspects of the molecular mechanism to be examined in detail. the homotetrameric enzymes belong to the type i family of extradiol ...200415028678
repeatability and reproducibility of ribotyping and its computer interpretation.many molecular typing methods are difficult to interpret because their repeatability (within-laboratory variance) and reproducibility (between-laboratory variance) have not been thoroughly studied. in the present work, ribotyping of coryneform bacteria was the basis of a study involving within-gel and between-gel repeatability and between-laboratory reproducibility (two laboratories involved). the effect of different technical protocols, different algorithms, and different software for fragment ...200415059627
the purification and characterisation of 4-chlorobenzoate:coa ligase and 4-chlorobenzoyl coa dehalogenase from arthrobacter sp. strain tm-1.4-chlorobenzoate:coa ligase, the first enzyme in the pathway for 4-chlorobenzoate dissimilation, has been partially purified from arthrobacter sp. strain tm-1, by sequential ammonium sulphate precipitation and chromatography on deae-sepharose and sephacryl s-200. the enzyme, a homodimer of subunit molecular mass approximately 56 kd, is dependent on mg2+-atp and coenzyme a, and produces 4-chlorobenzoyl coa and amp. besides mg2+, mn2+, co2+, fe2+ and zn2+ are also stimulatory, but not ca2+. maxima ...200415068371
effect of temperature and ph on the toxicity of aluminium towards two new, soil born species of arthrobacter sp.two coryneform bacteria arthrobacter sp. pi/1-95 (arth1) and arthrobacter sp. pi/3-95 (arth3) were isolated from forest soil characterized and investigated with respect to their reaction towards aluminium (al). sigmoid functions were used to describe dose-response relationships between al concentration and microbial growth and to calculate ec-values. ec(100) -- indicating a complete inhibition of microbial growth -- varied between 185 microm al for arth1 and 11 mm al for arth3. a pure ph effect ...200415069668
[employment of associative bacteria for the inoculation of barley plants cultivated in soil contaminated with lead and cadmium].in laboratory experiments, the rhizobacteria azospirillum lipoferum 137, arthrobacter mysorens 7, agrobacterium radiobacter 10, and flavobacterium sp. l30 were found to have a relatively high resistance to the toxic heavy metals lead and cadmium (except that strain l30 was found to be sensitive to cd). when introduced by means of seed bacterization, the heavy metal-resistant strains actively colonized the rhizosphere of barley plants cultivated in uncontaminated and contaminated soils. in both p ...200415074051
[a plate method to screen for microorganisms producing xylose isomerase].a plate method was developed to screen for xylose isomerase-producing microorganisms based on the use of 2,3,5-triphenyltetrazolium as an indicator of d-xylulose, the d-xylose isomerization product. the use of this method allows microorganisms to be differentiated by the character of the enzyme synthesis (inducible or constitutive).200415074052
effect of ph and inoculum size on phenol degradation by bacteria isolated from landfill waste.four aerobic species of mesophilic bacteria which catabolise phenol as the sole carbon source were isolated from landfill waste, and identified as acinetobacter, arthrobacter, micrococcus and nocardia spp. in vitro studies in defined medium revealed variations in abilities of the isolates to catabolise phenol, and in ph growth optima ranging from 6.8 to 7.6. the ph of culture media decreased with growth, and phenol catabolism was impeded below ph 5.4. phenol catabolism in landfill leachate occur ...198915092404
casuarina cunninghamiana tissue extracts stimulate the growth of frankia and differentially alter the growth of other soil microorganisms.aqueous extracts of host plant casuarina cunninghamiana tissue altered the in vitro growth of its diazotrophic microsymbiont frankia and a selection of other soil microorganisms. the growth of actinomycetous frankia strains, 55005. avci1, cesi5, cji82 001, and cj was stimulated by aqueous extracts of c. cunninghamiana tissue. green cladodes (photosynthetic branches), unsuberized roots, and suberized roots were more stimulatory than dry cladodes and seed tissue. aqueous extracts of green cladodes ...200415112734
efficacy of cellular vaccines and genetic adjuvants against bacterial kidney disease in chinook salmon (oncorhynchus tshawytscha).dna adjuvants and whole bacterial cell vaccines against bacterial kidney disease (bkd) were tested in juvenile chinook salmon. whole cell vaccines of either a nonpathogenic arthrobacter spp. or an attenuated renibacterium salmoninarum strain provided limited prophylactic protection against acute intraperitoneal challenge with virulent r. salmoninarum, and the addition of either synthetic oligodeoxynucleotides or purified r. salmoninarum genomic dna as adjuvants did not increase protection. howev ...200415123289
enzymatic synthesis of two novel non-reducing oligosaccharides using transfructosylation activity with beta-fructofuranosidase from arthrobacter globiformis.two novel non-reducing oligosaccharides together with tri- and tetra-saccharides were synthesized by transfructosylation activity from sucrose as a donor and cellobiose or cellotriose as an acceptor with a purified beta-fructofuranosidase from arthrobacter globiformis ifo 3062, and these oligosaccharides were identified as o-beta-d-glucopyranosyl-(1-->4)-alpha-d-glucopyranosyl-(1-->2)-alpha,beta-d-fructofuranoside and o-beta-d-glucopyranosyl-(1-->4)-beta-d-glucopyranosyl-(1-->4)-alpha-d-glucopyr ...200415127791
impact of temperature on the physiological status of a potential bioremediation inoculant, arthrobacter chlorophenolicus a6.arthrobacter chlorophenolicus a6 (a6) can degrade large amounts of 4-chlorophenol in soil at 5 and 28 degrees c. in this study, we investigated the effects of temperature on the physiological status of this bacterium in pure culture and in soil. a derivative of a6 tagged with the gfp gene (encoding green fluorescent protein [gfp]) was used to specifically quantify a6 cells in soil. in addition, cyano-ditolyl-tetrazoliumchloride was used to stain gfp-fluorescent cells with an active electron tran ...200415128556
arthrobacter nitroguajacolicus sp. nov., a novel 4-nitroguaiacol-degrading actinobacterium.three bacterial isolates from soil, capable of degradation or transformation of nitroaromatic compounds and displaying a rod-coccus growth cycle, were studied by a polyphasic approach. on the basis of 16s rrna sequence analysis and of chemotaxonomic characteristics, such as type a3alpha peptidoglycan with an interpeptide bridge ala-thr-ala, the major menaquinone mk-9(h(2)) and fatty acid composition, the isolates were assigned to the genus arthrobacter. dna-dna hybridization, riboprinting and ph ...200415143023
rothia aeria sp. nov., rhodococcus baikonurensis sp. nov. and arthrobacter russicus sp. nov., isolated from air in the russian space laboratory mir.four gram-positive bacteria, strains a1-17b(t), a1-22(t), a1-3(t) and a1-8, isolated from the air in the russian space laboratory mir, were subjected to a polyphasic taxonomic study. phylogenetic analysis of the bacteria based on their 16s rdna sequence showed that they belong to the genera rothia (a1-17b(t)), rhodococcus (a1-22(t)) and arthrobacter (a1-3(t) and a1-8). morphological, physiological, chemotaxonomic and genomic characteristics supported the assignments of these strains to these gen ...200415143031
biodegradation of maya crude oil fractions by bacterial strains and a defined mixed culture isolated from cyperus laxus rhizosphere soil in a contaminated site.ten bacterial strains were isolated by enrichment culture, using as carbon sources either aliphatics or an aromatic-polar mixture. oxygen uptake rate was used as a criterion to determine culture transfer timing at each enrichment stage. biodegradation of aliphatics (10,000 mg l(-1)) and an aromatic-polar mixture (5000 mg l(-1), 2:1) was evaluated for each of the bacterial strains and for a defined culture made up with a standardized mixture of the isolated strains. degradation of total hydrocarb ...200315162200
cytosolic nadp phosphatases i and ii from arthrobacter sp. strain km: implication in regulation of nad+/nadp+ balance.nadp phosphatase (nadpase) is an enzyme that converts nadp+ into nad+ through dephosphorylation of nadp+, and is considered to be one of the possible candidates for regulation of the nad+/nadp+ balance in vivo. in order to obtain an intrinsic nadpase, the nadp+-degrading activity in a membrane-free cell extract of a gram-positive bacterium, arthrobacter sp. strain km, was first assessed and demonstrated to be mainly achieved through the nadpase reaction, indicating nadpase is essential for degra ...200415162392
characterization of endo-beta-n-acetylglucosaminidase from alkaliphilic bacillus halodurans c-125.the genome sequencing project on alkaliphilic bacillus halodurans c-125 revealed a putative endo-beta-n-acetylglucosaminidase (endo-bh), which consists of a signal peptide of 24 amino acids, a catalytic region of 634 amino acids exhibiting 50.1% identity with the endo-beta-n-acetylglucosaminidase from arthrobacter protophormiae (endo-a), and a c-terminal tail of 220 amino acids. transformed escherichia coli cells carrying the endo-bh gene exhibited endo-beta-n-acetylglucosaminidase activity. rec ...200415170110
cytoplasmic membrane polarization in gram-positive and gram-negative bacteria grown in the absence and presence of tetracycline.the ability of numerous diverse compounds and ions to cross the bacterial cytoplasmic membrane by diffusion and active transport is highly dependent on cytoplasmic membrane fluidity, which can be measured using fluorescent probes to estimate membrane polarization values. however, membrane polarization data are lacking for most bacterial species. the cytoplasmic membrane polarization values for arthrobacter sp. atcc 21908, bacillus cereus nrc 3045, pseudomonas fluorescens r2f, pseudomonas putida ...200415182931
site-directed mutagenesis establishes aspartic acids-227 and -342 as essential for enzyme activity in an isomalto-dextranase from arthrobacter globiformis.isomalto-dextranase, from arthrobacter globiformis t6, is a member of the glycoside hydrolase family 27. however, the alignments of the whole amino acid sequence are distinct from other members of this family. the enzymes cleave the glycosidic bond of the substrate in two different manners: either retaining or inverting the anomeric configuration. we believe that a retaining enzyme is involved in a two-step, double-displacement mechanism utilizing active site carboxylic acids as the nucleophile ...200415200177
immunologic reactivity to work-related airborne allergens in people occupationally exposed to dust from herbs.a group of 150 people occupationally exposed to dust from herbs were examined. the examined group consisted of 47 thyme farmers, 32 chamomile farmers, 31 sage farmers and 40 workers of herbs processing industry. as a reference group, 50 urban dwellers, not exposed to any kind of organic dust, were examined. skin prick tests and precipitin tests were conducted with, respectively, 4 and 11 microbial antigens associated with organic dust. both skin and precipitin tests were also conducted with herb ...200415236509
geomicrobiology of high-level nuclear waste-contaminated vadose sediments at the hanford site, washington state.sediments from a high-level nuclear waste plume were collected as part of investigations to evaluate the potential fate and migration of contaminants in the subsurface. the plume originated from a leak that occurred in 1962 from a waste tank consisting of high concentrations of alkali, nitrate, aluminate, cr(vi), (137)cs, and (99)tc. investigations were initiated to determine the distribution of viable microorganisms in the vadose sediment samples, probe the phylogeny of cultivated and uncultiva ...200415240306
arthrobacter aurescens tc1 atrazine catabolism genes trzn, atzb, and atzc are linked on a 160-kilobase region and are functional in escherichia coli.arthrobacter aurescens strain tc1 metabolizes atrazine to cyanuric acid via trzn, atzb, and atzc. the complete sequence of a 160-kb bacterial artificial chromosome clone indicated that trzn, atzb, and atzc are linked on the a. aurescens genome. trzn, atzb, and atzc were shown to be functional in escherichia coli. hybridization studies localized trzn, atzb, and atzc to a 380-kb plasmid in a. aurescens strain tc1.200415240330
hyper-production of an isomalto-dextranase of an arthrobacter sp. by a proteases-deficient bacillus subtilis: sequencing, properties, and crystallization of the recombinant enzyme.arthrobacter globiformis t6 is unique in that it produces an enzyme yielding only isomaltose from dextran. in the present study, the organism was re-identified and its classification as a new species of the genus arthrobacter, a. dextranlyticum, was proposed. the high g+c gene (66.8 mol%) for the isomalto-dextranase was sequenced. the deduced amino acid sequence, with a calculated molecular mass of 65,993 da (603 amino acids), was confirmed by nanoscale capillary liquid chromatography coupled to ...200415248038
engineering root exudation of lotus toward the production of two novel carbon compounds leads to the selection of distinct microbial populations in the rhizosphere.the culture of opine-producing transgenic lotus plants induces the increase in the rhizosphere of bacterial communities that are able to utilize these molecules as sole carbon source. we used transgenic lotus plants producing two opines, namely mannopine and nopaline, to characterize the microbial communities directly influenced by the modification of root exudation. we showed that opine-utilizers represent a large community in the rhizosphere of opine-producing transgenic lotus. this community ...200415259274
a novel p-nitrophenol degradation gene cluster from a gram-positive bacterium, rhodococcus opacus sao101.p-nitrophenol (4-np) is recognized as an environmental contaminant; it is used primarily for manufacturing medicines and pesticides. to date, several 4-np-degrading bacteria have been isolated; however, the genetic information remains very limited. in this study, a novel 4-np degradation gene cluster from a gram-positive bacterium, rhodococcus opacus sao101, was identified and characterized. the deduced amino acid sequences of npcb, npca, and npcc showed identity with phenol 2-hydroxylase compon ...200415262926
[phylogenetic analysis of alpha-galactosidases of the gh27 family].amino acid sequence analysis of alpha-galactosidases and other proteins of glycoside hydrolase family 27 (gh27) allowed isolation of three major subfamilies, 27a-27c. unique isomalto-dextranase of arthrobacter globiformis clustered separately. eukaryotic proteins formed five clusters on a phylogenetic tree of the family. bacterial gh27 proteins, which are relatively few, did not form stable clusters. a monophyletic origin of the gh27 family was demonstrated with the use of related proteins of th ...200415285616
oligosaccharides and derivatives--integrating biocatalyst selectivity and chemical diversity.several biocatalytic pathways are available to synthesize rare sugars, oligosaccharides and derivatives. as examples selective hydrolysis, transglycosylation and regioselective oxidation are presented illustrating their potential. from inulin, difructose anhydrides and inulobiose are accessible via specific hydrolases with transglycosylation activity. glycosyltransferases of the sucrase type utilise sucrose as a versatile substrate for the selective transfer of either glucosyl- or fructosyl unit ...200315296178
crystallization and preliminary x-ray analysis of a hydantoinase from arthrobacter aurescens dsm 3745.l-hydantoinase from arthrobacter aurescens dsm 3745 has been purified to homogeneity and crystallized from polyethylene glycol solutions in a form suitable for x-ray diffraction analysis. the crystals have been grown by the sitting-drop variant of the vapour-diffusion method. x-ray diffraction studies show that the crystals belong to the monoclinic space group p2(1) with a = 111.2, b = 74.4, c = 146.5 a and beta = 106.7 degrees. its asymmetric unit contains four monomers related by 222 symmetry. ...199615299588
purification, crystallization and preliminary x-ray crystal structure analysis of copper amine oxidase from arthrobacter globoformis.beta-phenylethylamine oxidase from the gram-positive bacterium arthrobacter globoformis has been crystallized as three crystal forms. two belong to space group c2 and one to space group p2(1)2(1)2(1), respectively. the unit-cell volumes are consistent with one infunit of 70 644 da per asymmetric unit for the two monoclinic forms, and with two infunits per asymmetric unit for the orthorhombic crystals. three-dimensional intensity data have been recorded to 2.8a resolution for one of the monoclini ...199615299744
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