purification and characterization of aminobutyraldehyde dehydrogenase from arthrobacter sp. tmp-1.aminobutyraldehyde dehydrogenase was purified to essentially homogeneity from putrescine-grown cells of arthrobacter sp. tmp-1. the molecular weights of the enzyme and its subunit were 201,000 and 51,000, respectively, suggesting that the enzyme is a tetramer of identical subunits. the apparent michaelis constants (k(m)) for 4-aminobutyraldehyde, 3-aminopropionaldehyde and 4-guanidinobutyraldehyde were approximately 65, 150, and 85 microm, respectively. linear fatty aldehydes also tested were le ...200212186751
capillary electrophoresis of cr(vi) reducer arthrobacter oxydans.rapid and effective separation of bacteria arthrobacter oxydans was performed using capillary electrophoresis. for optimal separation of bacteria the influence of buffer concentration, ph and applied voltage were studied. it was found that the most appropriate conditions for electrophoretic mobility measurements are as follows: applied voltage 6-14 kv; buffer concentration 5-10 mm ph 6-8. at the stationary phase of growth there are always two main heterogeneous peaks. they are connected with the ...200212210506
cultivation-dependent characterization of bacterial diversity from british columbia forest soils subjected to disturbance.bacteria from forest surface organic matter and mineral soil horizons were cultivated using four methods and characterized by fatty acid methyl ester (fame) analysis. soil samples from a british columbia ministry of forests long-term soil productivity (ltsp) installation were collected during winter and summer from two disturbance treatments (whole-tree harvesting with no soil compaction (plot n) and whole-tree harvesting plus complete surface organic matter removal with heavy soil compaction (p ...200212224563
bioremediation of an industrial effluent containing monocrotophos.almost 30% of the precious agricultural output of india is lost owing to pest infestation. in india, pesticide consumption for protecting crops is about 3% of the total world consumption. monocrotophos (mcp), an organophosphorus insecticide, is widely used to control insects on crops. being readily water soluble and highly toxic, its removal from wastewater generated during manufacture becomes inevitable. bioremediation of wastewater containing mcp by arthrobacter atrocyaneus, bacillus megateriu ...200212232665
intact golgi synthesize complex branched o-linked chains on glycoside primers: evidence for the functional continuity of seven glycosyltransferases and three sugar nucleotide transporters.we examined the functional co-localization and continuity of glycosyltransferases and sugar nucleotide transporters in the golgi of two chinese hamster ovary (cho) cell lines that synthesize different types of o-linked oligosaccharides. cho cells normally synthesize primarily sia2,3galbeta1,3galnac- on glycoproteins. cho cells transfected with core-2 glcnac transferase (core 2) can synthesize glycoproteins containing branched o-linked oligosaccharides with poly-n-acetyllactosamines. cho lines in ...200112376727
aari, a restriction endonuclease from arthrobacter aurescens ss2-322, which recognizes the novel non-palindromic sequence 5'-cacctgc(n)4/8-3'.a new type ii restriction endonuclease aari has been isolated from arthrobacter aurescens ss2-322. aari recognizes the non-palindromic heptanucleotide sequence 5'-cacctgc(n)4/8-3' and makes a staggered cut at the fourth and eighth bases downstream of the target duplex producing a four base 5'-protruding end. aari activity is stimulated by oligodeoxyribonucleotide duplexes containing an enzyme-specific recognition sequence.200212409482
arthrobacter aurescens tc1 metabolizes diverse s-triazine ring compounds.arthrobacter aurescens strain tc1 was isolated without enrichment by plating atrazine-contaminated soil directly onto atrazine-clearing plates. a. aurescens tc1 grew in liquid medium with atrazine as the sole source of nitrogen, carbon, and energy, consuming up to 3,000 mg of atrazine per liter. a. aurescens tc1 is metabolically diverse and grew on a wider range of s-triazine compounds than any bacterium previously characterized. the 23 s-triazine substrates serving as the sole nitrogen source i ...200212450818
impact of protozoan grazing on bacterial community structure in soil microcosms.the influence of grazing by a mixed assemblage of soil protozoa (seven flagellates and one amoeba) on bacterial community structure was studied in soil microcosms amended with a particulate resource (sterile wheat roots) or a soluble resource (a solution of various organic compounds). sterilized soil was reinoculated with mixed soil bacteria (obtained by filtering and dilution) or with bacteria and protozoa. denaturing gradient gel electrophoresis (dgge) of pcr amplifications of 16s rrna gene fr ...200212450833
high affinity iron-uptake systems in vibrio damsela: role in the acquisition of iron from this work, the high affinity iron-acquisition systems displayed by virulent and avirulent strains of vibrio damsela have been investigated. this species is an autochthonous member of marine ecosystems that can behave as an opportunistic pathogen for fish and mammals. all strains tested (i) were able to grow under the restricted conditions imposed by the iron chelators transferrin (tf) and eddha, (ii) secreted siderophores of hydroxamic type, other than aerobactin and desferal, that were able ...199712452589
isolation of arthrobacter strains on the basis of their cystite formation. 200212469299
the formation and structures of cystites of arthrobacter ureafaciens nric 0157t induced by antibiotics.the cystite formation of arthrobacter ureafaciens nric 0157(t) was induced by some antibiotics, and the addition of tetracycline at a lag phase was effective for cystite formation in ypm liquid medium. cystites differed from vegetative cells in the cell wall structure, protein content, and water content. furthermore, the characteristics and structures of cystites induced by tetracycline were similar to those of cystites produced by nutritional imbalance in ct medium. consequently, various trigge ...200112483560
cystite formation of arthrobacter ureafaciens nric 0157(t).the cystite formation of arthrobacter ureafaciens nric 0157(t) was studied by the use of a newly designed ct medium composed of 2.0% d-glucose, 0.28% nh(4)h(2)po(4), 0.136% k(2)hpo(4) 0.136% kh(2)po(4), 0.0005% mgso(4).7h(2)o, and 0.0007% cacl(2).2h(2)o. cystites are drumstick-shaped and oval cells and are larger than vegetative cells. cystites are gram-negative, whereas vegetative cells are gram-positive by the koh reaction. the concentration and ratio of k(+) and mg(2+) in ct medium mainly aff ...200012483589
metabolism of gamma-hexachlorocyclohexane by arthrobacter citreus strain bi-100: identification of metabolites.growth characteristics of the aerobic bacterial strain arthrobacter citreus bi-100 in mineral salts medium with gamma-hexachlorocyclohexane (gamma-hch) as the sole source of carbon and degradation of gamma-hch by the strain are reported. the highest yield of the bacteria is observed at a gamma-hch concentration of 100 mg/l. at this concentration, the bacteria entered the exponential phase of growth without any lag. at 8 h of growth, no residual hch, but its metabolites, was detectable in the med ...200012483592
characterization of the 4-carboxy-4-hydroxy-2-oxoadipate aldolase gene and operon structure of the protocatechuate 4,5-cleavage pathway genes in sphingomonas paucimobilis syk-6.the protocatechuate (pca) 4,5-cleavage pathway is the essential metabolic route for degradation of low-molecular-weight products derived from lignin by sphingomonas paucimobilis syk-6. in the 10.5-kb ecori fragment carrying the genes for pca 4,5-dioxygenase (ligab), 2-pyrone-4,6-dicarboxylate hydrolase (ligi), 4-oxalomesaconate hydratase (ligj), and a part of 4-carboxy-2-hydroxymuconate-6-semialdehyde dehydrogenase (ligc), we found the ligk gene, which encodes 4-carboxy-4-hydroxy-2-oxoadipate (c ...200312486039
simultaneous horizontal gene transfer of a gene coding for ribosomal protein l27 and operational genes in arthrobacter sp.phylogenetic analysis of bacterial l27 ribosomal proteins showed that, against taxonomy, the l27 protein from the actinobacteria arthrobacter sp. clusters with protein sequences from the bacillus group. the l27 gene clusters in the arthrobacter sp. genome with six genes responsible for creatinine and sarcosine degradation. phylogenetic analyses of orthologue proteins encoded by three of these genes also showed a phylogenetic relationship with bacillus species. comparisons between the synonymous ...200212486522
exposure to airborne microorganisms and endotoxin in a potato processing plant.microbiological air sampling was performed in a big potato processing plant located in eastern poland. air samples for determination of concentrations of microorganisms, dust and endotoxin were collected at 6 sites in the division producing potato flakes and meal from dried potato pulp and at 2 sites in the division producing potato syrup from imported starch. the concentrations of total airborne microorganisms were within a range of 28.3-93.1 x 10(3) cfu/m(3). mesophilic bacteria were dominant ...200212498592
[mercury-resistant bacteria from permafrost sediments and prospects for their use in comparative studies of mercury resistance determinants].mercury-resistant bacteria were isolated from permafrost sediments of kolyma lowland and canada existing over five thousand to two million years. their content was shown to vary within the range 0.001-2.9% and to depend on the amount of mercury in sampling sites (coefficient of correlation 0.75). a collection of mercury-resistant bacterial strains was created. in this collection, various representatives of both gram-positive bacteria (bacillus, exiguobacterium, micrococcus, arthrobacter) and gra ...200212500685
biodegradation of the mixture of 2,4,6-trichlorophenol, 4-chlorophenol, and phenol by a defined mixed culture.two new strains, pseudomonas sp. tcp114 degrading 2,4,6-trichlorophenol (tcp) and arthrobacter sp. cpr706 degrading 4-chlorophenol (4-cp), were isolated through a selective enrichment procedure. both strains could also degrade phenol. the degradability of one component by a pure culture was strongly affected by the presence of other compounds in the medium. for example, when all three components (tcp, 4-cp, and phenol) were present in the medium, a pure culture of cpr706 could not degrade any of ...199712501340
novel reversible indole-3-carboxylate decarboxylase catalyzing nonoxidative decarboxylation.after enrichment culture with indole-3-carboxylate in static culture, a novel reversible decarboxylase, indole-3-carboxylate decarboxylase, was found in arthrobacter nicotianae fi1612 and several molds. the enzyme reaction was examined in resting-cell reactions with a. nicotianae fi1612. the enzyme activity was induced specifically by indole-3-carboxylate, but not by indole. the indole-3-carboxylate decarboxylase of a. nicotianae fi1612 catalyzed the nonoxidative decarboxylation of indole-3-carb ...200212506977
citricoccus muralis gen. nov., sp. nov., a novel actinobacterium isolated from a medieval wall painting.a gram-positive, aerobic, spherical actinobacterium, designated strain 4-0(t), was isolated from a medieval wall painting and characterized to determine its taxonomic position. the peptidoglycan of strain 4-0(t) was of type a4alpha, with lysine as the diagnostic cell wall diamino acid and an interpeptide bridge of lys-gly-glu. its quinone system contained predominantly mk-9(h2) (64%) and its polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, four ...200212508874
mrna differential display in a microbial enrichment culture: simultaneous identification of three cyclohexanone monooxygenases from three species.mrna differential display has been used to identify cyclohexanone oxidation genes in a mixed microbial community derived from a wastewater bioreactor. thirteen dna fragments randomly amplified from the total rna of an enrichment subculture exposed to cyclohexanone corresponded to genes predicted to be involved in the degradation of cyclohexanone. nine of these dna fragments are part of genes encoding three distinct baeyer-villiger cyclohexanone monooxygenases from three different bacterial speci ...200312514013
role of copper ion in bacterial copper amine oxidase: spectroscopic and crystallographic studies of metal-substituted enzymes.the role of the active site cu(2+) of phenylethylamine oxidase from arthrobacter globiformis (agao) has been studied by substitution with other divalent cations, where we were able to remove >99.5% of cu(2+) from the active site. the enzymes reconstituted with co(2+) and ni(2+) (co- and ni-agao) exhibited 2.2 and 0.9% activities, respectively, of the original cu(2+)-enzyme (cu-agao), but their k(m) values for amine substrate and dioxygen were comparable. x-ray crystal structures of the co- and n ...200312537504
microbial diversity and resistance to copper in metal-contaminated lake sediment.contamination of habitats with heavy metals has become a worldwide problem. we describe herein the analysis of lake sediment contaminated with high concentrations of copper as a consequence of mine milling disposal over a 100-year period. copper concentrations in the sediment were found to vary with depth and ranged from 200 to 5500 ppm. analysis of the microbial community with t-rflp identified a minimum of 20 operational taxonomic units (otu). t-rflp analysis along a depth profile detected as ...200312545313
[microbial succession on lignite along with weathering].six different weathered lignite samples were examined by scanning electron microscopy. few microorganisms were observed on lignit just excavated and only spores and short hyphae were observed on lignite samples excavated 5 months, 1 year and 4 years before sampling. when lignite samples were moistened with distilled water and incubated for 10 days, actinomycetes proliferated significantly on lignite samples that were just excavated or excavated 5 months before sampling. the growth of bacteria wa ...199812548918
[studies on hydantoinase producing conditions of arthrobacter k1108].the hydantoinase-producing conditions from strain arthrobacter k1108 were investigated. it is shown that the hydantoinase in the strain is intracellular and inducible. its optimal inducer is 5-benzylhydantoin, while 5-indolylmethylhydantoin and 5-phenylhydantoin cannot induce the production of hydantoinase in k1108. a gratuitous inducer was designed, with which the hydantoinase production is 343% as much as that with 5-benzylhydantoin. the media for culturing the bacteria were screened and optim ...200212557399
[the solubilization of four insoluble phosphates by some microorganisms].four insoluble phosphates of ferric phosphate (fe-p), aluminum phosphate (al-p), fluorapatite (fap) and rock phosphate (rp) were used as a sole phosphorus resource for some phosphate-solubilizing microorganisms. it was found that there was significant difference in solubilizing these phosphates by the tested isolates. the fungi normally were more powerful than the bacteria in dissolving the phsophates. the microorganisms generally solubilized more phosphate when supplied with no3- than with nh4+ ...200212557403
chemoenzymatic synthesis of high-mannose type hiv-1 gp120 glycopeptides.a chemoenzymatic approach to the synthesis of glycoforms of hiv-1 gp120 glycopeptides is described. thus, the high-mannose type glycopeptides [gp120 (336-342)] containing man(9), man(6) and man(5) moieties, respectively, were synthesized in satisfactory yields via transglycosylation to the acetylglucosaminyl peptide, using the recombinant arthrobacter endo-beta-n-acetyl-glucosaminidase (endo-a) as the key enzyme.200312565922
evaluation of broiler litter with reference to the microbial composition as assessed by using 16s rrna and functional gene markers.very little is known about the microbial composition of animal bedding wastes, including poultry litter, and what is known has been deduced from standard culture methods, by which some fastidious organisms that exist in the environment may not be detected. we evaluated the bacterial composition of poultry litter by using a combination of culture and molecular detection. total aerobic bacteria in poultry litter were detected by culture at 10(9) cfu/g of material. enteric bacteria such as enteroco ...200312571010
arthrobacter strain vai-a utilizes acyl-homoserine lactone inactivation products and stimulates quorum signal biodegradation by variovorax paradoxus.many proteobacteria produce acyl-homoserine lactones (acyl-hsls) and employ them as dedicated cell-to-cell signals in a process known as quorum sensing. previously, variovorax paradoxus vai-c was shown to utilize diverse acyl-hsls as sole sources of energy and nitrogen. we describe here the properties of a second isolate, arthrobacter strain vai-a, obtained from the same enrichment culture that yielded v. paradoxus vai-c. although strain vai-a grew rapidly and exponentially on a number of substr ...200312571011
[microbial destruction of harmful organic contaminants inherent to atmosphere of living space].the investigation into the microbial destruction of harmful organic contaminants in the air of habitable rooms was performed with the assumption that microorganisms have labile metabolism and synthesize inducible enzymes involved in transformation of organics compounds. based on our observations, methylobacterium d-08 and arthrobacter pastens are able to synthesize inducible enzymes and transform ethanol, acetic acid, acetone, and aldehyde. association of these microorganisms does not reveal acu ...200212572127
transgenics of an elite indica rice variety pusa basmati 1 harbouring the coda gene are highly tolerant to salt stress.transgenic lines of indica rice were generated by agrobacterium-mediated transformation with the choline oxidase ( coda) gene from arthrobacter globiformis. choline oxidase catalyses conversion of choline to glycine betaine. glycine betaine is known to provide tolerance against a variety of stresses. molecular analyses of seven independent transgenic lines as performed by southern, northern and western hybridization revealed integration and expression of the transgene as well as inheritance in t ...200212582870
studies on the effect of inoculation of activated sludge with bacteria actively degrading hydrocarbons on the biodegradation of petroleum products.eighteen strains of bacteria were isolated from activated sludge purifying petroleum-refining wastewaters. these strains were plated on solidified mineral medium supplemented with oil fraction in concentration 1000 mg/l. four of the strains that grew best in the presence of oil were selected for further studies. the strains were identified based on bonde's scheme and microscopic observations. three of them belonged to the genus arthrobacter and one to the genus micrococcus. stationary cultures o ...200212588103
4-nitrocatechol as a probe of a mn(ii)-dependent extradiol-cleaving catechol dioxygenase (mndd): comparison with relevant fe(ii) and mn(ii) model 3,4-dihydroxyphenylacetate 2,3-dioxygenase (mndd) is an extradiol-cleaving catechol dioxygenase from arthrobacter globiformis that has 82% sequence identity to and cleaves the same substrate (3,4-dihydroxyphenylacetic acid) as fe(ii)-dependent 3,4-dihydroxyphenylacetate 2,3-dioxygenase (hpcd) from brevibacterium fuscum. we have observed that mndd binds the chromophoric 4-nitrocatechol (4-nch(2)) substrate as a dianion and cleaves it extremely slowly, in contrast to the fe(ii)-de ...200312589562
sequence of the 165-kilobase catabolic plasmid pao1 from arthrobacter nicotinovorans and identification of a pao1-dependent nicotine uptake system.the 165-kb catabolic plasmid pao1 enables the gram-positive soil bacterium arthrobacter nicotinovorans to grow on the tobacco alkaloid l-nicotine. the 165,137-nucleotide sequence, with an overall g+c content of 59.7%, revealed, besides genes and open reading frames (orfs) for nicotine degradation, a complete set of orfs for enzymes essential for the biosynthesis of the molybdenum dinucleotide cofactor, as well as orfs related to uptake and utilization of carbohydrates, sarcosine, and amino acids ...200312618462
respiration of 13c-labeled substrates added to soil in the field and subsequent 16s rrna gene analysis of 13c-labeled soil dna.our goal was to develop a field soil biodegradation assay using (13)c-labeled compounds and identify the active microorganisms by analyzing 16s rrna genes in soil-derived (13)c-labeled dna. our biodegradation approach sought to minimize microbiological artifacts caused by physical and/or nutritional disturbance of soil associated with sampling and laboratory incubation. the new field-based assay involved the release of (13)c-labeled compounds (glucose, phenol, caffeine, and naphthalene) to soil ...200312620850
predominant bacillus spp. in agricultural soil under different management regimes detected via pcr-dgge.a pcr system for studying the diversity of species of bacillus and related taxa directly from soil was developed. for this purpose, a specific 24-bp forward primer located around position 110 of the 16s ribosomal rna gene was designed and combined with a reverse bacterial primer located at the end of the gene. the specificity of this pcr system for bacilli and related taxons was confirmed on the basis of tests with diverse strains as well as with soil dna. analysis of a soil dna derived clone li ...200312632212
kinetic mechanism of choline oxidase from arthrobacter globiformis.choline oxidase catalyzes the four-electron oxidation of choline to glycine-betaine, with betaine-aldehyde as intermediate and molecular oxygen as primary electron acceptor. the enzyme is capable of accepting betaine-aldehyde as a substrate, allowing the investigation of the reaction mechanism for both the conversion of choline to the aldehyde intermediate and of betaine-aldehyde to glycine-betaine. the steady state kinetic mechanism has been determined at ph 7 with choline and betaine-aldehyde ...200312637017
proteolytic activity of a yeast cell wall lytic arthrobacter investigate properties of the proteolytic activity of a yeast cell wall lytic soil bacterium identified as an arthrobacter species.200312641716
[the existence of contamination in enzymes used for the isolation of aspergillus and candida species dna].molecular biological methods which are widely used in different fields, have been replaced with conventional diagnostic tests for the early diagnosis of invasive fungal infections, recently. polymerase chain reaction (pcr) is one of these methods, with high specificity and sensitivity, which is accepted throughout the world. however, the enzymes that are used for the isolation of target dna, may be contaminated with the gene sequences of some other fungal species in the preparation steps and may ...200212652873
dimethylphthalate hydrolysis by specific microbial esterase.two bacterial strains: arthrobacter sp. and sphingomonas paucimobilis were isolated from soil by enrichment cultures using dimethylphthalate (dmp) or monomethylphthalate (mmp) as sole carbon source, respectively. dmp was rapidly transformed by an arthrobacter sp. culture with formation of mmp and phthalic acid (pa) which is further degraded. this strain was unable to hydrolyse mmp. a mechanism of degradation of dmp was proposed with two ways: dmp-->pa and dmp-->mmp. the s. paucimobilis strain hy ...200312668024
agar-plated bacteria found in the activated sludge of lab-scale sbr and cfstr systems.we identified and compared the predominant agar-plated bacteria in the activated sludge of two popular wastewater treatment systems, the sequencing batch reactor (sbr) and the continuous-flow stirred tank reactor (cfstr). all tests had the same feed composition except for the buffer intensity. it was found that corynebacterium sp. seemed to prefer growing under lower buffer condition, and arthrobacter sp. grew dominantly in the system with higher buffer intensity. gram-negative bacteria appeared ...200312676503
isolation and characterization of an atrazine-degrading bacterium from industrial wastewater in isolate and characterize atrazine-degrading bacteria in order to identify suitable candidates for potential use in bioremediation of atrazine contamination.200312680937
inhibition of six copper-containing amine oxidases by the antidepressant drug tranylcypromine.potential inhibitory effects of the clinically utilized monoamine oxidase inhibitor tranylcypromine (tcp) on mammalian, plant, bacterial, and fungal copper-containing amine oxidases have been examined. the following enzymes have been investigated: human kidney diamine oxidase (hkao), bovine plasma amine oxidase (bpao), equine plasma amine oxidase (epao), pea seedling amine oxidase (psao), arthrobacter globiformis amine oxidase (agao), and pichia pastoris lysyl oxidase (pplo). only bpao, epao, an ...200312686142
process for producing the potential food ingredient dfa iii from inulin: screening, genetic engineering, fermentation and immobilisation of inulase ii.difructose anhydride (dfa iii) is a new potential sweet food additive. a screening was undertaken to isolate bacterial strains for conversion of inulin to dfa. of special interest were thermotolerant enzymes. some 400 strains were investigated, among four of them produce dfa and strain buo141 expresses an extracellular enzyme which is stable at elevated temperatures. based on metabolic data and 16s-rrna-sequencing, the strain was identified as a new arthrobacter species. for increased enzyme pro ...200312695027
levan fructotransferase from arthrobacter oxydans j17-21 catalyzes the formation of the di-d-fructose dianhydride iv from levan.a new levan fructotransferase (lftase) isolated from arthrobacter oxydans j17-21 was characterized for the production of difructose dianhydride iv (dfa iv). lftase was purified to apparent homogeneity by q-sepharose ion exchange chromatography, mono-q hr 5/5 column chromatography, and gel permeation chromatography. the enzyme had an apparent molecular mass of 54000 da. the optimum ph for the enzyme-catalyzed reaction was ph 6.5, and the optimum temperature was observed at 45 degrees c. the lftas ...200312696949
[the 1(2)-dehydrogenation of steroid substrates by nocardioides simplex vkm ac-2033d].the bacterium formerly known as arthrobacter globiformis 193 has high 1(2)-dehydrogenase activity toward pharmaceutically important steroids, 9(11)-dehydrocortexolone in particular. the complex analysis of the morphostructural, physiological, biochemical, and phylogenetic properties of this bacterium allowed us to reclassify it into nocardioides simplex (n. simplex vkm ac-2033d).200312698789
metal resistance among aerobic chemoheterotrophic bacteria from the deep terrestrial subsurface.the metal resistance of 350 subsurface bacterial strains from two u.s. department of energy facilities, the savannah river site (srs), south carolina, and the hanford site, washington, was determined to assess the effect of metal toxicity on microorganisms in the deep terrestrial subsurface. resistance was measured by growth inhibition around discs containing optimized amounts of hg(ii), pb(ii), and cr(vi). a broad range of resistance levels was observed, with some strains of arthrobacter spp. d ...200312718404
gene cluster of arthrobacter ilicis ru61a involved in the degradation of quinaldine to anthranilate: characterization and functional expression of the quinaldine 4-oxidase qoxlms genes.a genetic analysis of the anthranilate pathway of quinaldine degradation was performed. a 23-kb region of dna from arthrobacter ilicis rü61a was cloned into the cosmid pvk100. although escherichia coli clones containing the recombinant cosmid did not transform quinaldine, cosmids harboring the 23-kb region, or a 10.8-kb stretch of this region, conferred to pseudomonas putida kt2440 the ability to cometabolically convert quinaldine to anthranilate. the 10.8-kb fragment thus contains the genes cod ...200312730200
characterization of the 4-hydroxybenzoyl-coenzyme a thioesterase from arthrobacter sp. strain su.the arthrobacter sp. strain su 4-chlorobenzoate (4-cba) dehalogenation pathway converts 4-cba to 4-hydroxybenzoate (4-hba). the pathway operon contains the genes fcba, fcbb, and fcbc (a. schmitz, k. h. gartemann, j. fiedler, e. grund, and r. eichenlaub, appl. environ. microbiol. 58:4068-4071, 1992). genes fcba and fcbb encode 4-cba-coenzyme a (coa) ligase and 4-cba-coa dehalogenase, respectively, whereas the function of fcbc is not known. we subcloned fcbc and expressed it in escherichia coli, a ...200312732540
[the effect of sulfur on the hydrocarbon-oxidizing bacteria of different genera growth]. 200312751253
d-arginase of arthrobacter sp. kuj 8602: characterization and its identity with zn(2+)-guanidinobutyrase.d-arginase activity was found in the cells of an isolate, arthrobacter sp. kuj 8602, grown in the l-arginine medium, and the enzyme was purified and characterized. its molecular weight was estimated to be about 232,000 by gel filtration, and that of the subunit was approximately 40,000 by sds-page, suggesting that the enzyme is a homohexamer. the enzyme acted on not only d-arginine but also 4-guanidinobutyrate, 3-guanidinopropionate and even l-arginine. the v(max)/k(m) values for 4-guanidinobuty ...200312761196
a set of glycoproteins recognized by a2b5 antibody with major bands at 55 and 76 kda is overexpressed in human head and neck squamous cell carcinomas.a2b5 antibody was found to strongly label frozen sections of human head and neck squamous cell carcinomas. the low amount of glycolipids (c-series gangliosides and sulfatides) purified from the same tumors and reactive with a2b5 by immunostaining on thin-layer plates could not account for the high level of tissue labeling. proteins were extracted from both normal tissues and squamous cell carcinomas and analyzed by western blot with a2b5 antibody on pvdf membranes. the antibody was found to stai ...200312770779
ahld, an n-acylhomoserine lactonase in arthrobacter sp., and predicted homologues in other bacteria.quorum sensing is a signalling mechanism that controls diverse biological functions, including virulence, via n-acylhomoserine lactone (ahl) signal molecules in gram-negative bacteria. with the aim of isolating strains or enzymes capable of blocking quorum sensing by inactivating ahl, bacteria were screened for ahl degradation by their ability to utilize n-3-oxohexanoyl-l-homoserine lactone (ohhl) as the sole carbon source. among four isolates, strain ibn110, identified as arthrobacter sp., was ...200312777494
toxicity of hexavalent chromium and its reduction by bacteria isolated from soil contaminated with tannery arthrobacter sp. and a bacillus sp., isolated from a long-term tannery waste contaminated soil, were examined for their tolerance to hexavalent chromium [cr(vi)] and their ability to reduce cr(vi) to cr(iii), a detoxification process in cell suspensions and cell extracts. both bacteria tolerated cr(vi) at 100 mg/ml on a minimal salts agar medium supplemented with 0.5% glucose, but only arthrobacter could grow in liquid medium at this concentration. arthrobacter sp. could reduce cr(vi) up to 5 ...200312783193
structural characterization and mapping of the covalently linked fad cofactor in choline oxidase from arthrobacter globiformis.the flavoenzyme choline oxidase catalyzes the oxidation of choline and betaine aldehyde to betaine. earlier studies have shown that the choline oxidase from arthrobacter globiformis contains fad covalently linked to a histidine residue. to identify the exact type of flavin binding, the fad-carrying amino acid residue was released by acid hydrolysis. the fluorescence excitation maxima of the isolated aminoacylriboflavin, showing a hypsochromic shift of the near-ultraviolet band relative to ribofl ...200312795615
gold electrodes wired for coupling with the deeply buried active site of arthrobacter globiformis amine oxidase.diethylaniline-terminated oligo(phenyl-ethynyl)-thiol (dea-ope-sh) wires on au-bead electrodes facilitate electron tunneling to and from the deeply buried topaquinone (tpq) cofactor in arthrobacter globiformis amine oxidase (agao). reversible cyclic voltammograms were observed when agao was adsorbed onto this dea-ope-sau surface: the 2e-/2h+ reduction potential is -140 mv versus sce.200312797771
a calorimetric characterization of cr(vi)-reducing arthrobacter oxydans at different phases of the cell growth cycle.this is the first of a series of calorimetric studies designed to characterize and understand survival mechanisms of metal-reducing bacteria isolated from metal-polluted environments. in this paper we introduce a new concept of thermal spectrum of the endothermic melting of complex biological systems (e.g., proteins, nucleic acids, ribosomes, membrane structures) in intact cells. all thermal spectra measured are thermograms that describe the temperature dependence of heat capacity change of the ...200312806104
naphthalene-utilizing and mercury-resistant bacteria isolated from an acidic environment.soil samples were taken from areas of low ph (2.5-3.5) surrounding an outdoor coal storage pile. these samples were added to medium with naphthalene as the sole carbon source to enrich for organisms capable of degrading polycyclic aromatic hydrocarbons (pah) at low ph. five such bacterial strains were isolated. sequencing of the 16s rdna showed them to be members of the genera clavibacter, arthrobacter and acidocella. these organisms were all capable of growth with naphthalene as a sole carbon s ...200312827325
members of the genus arthrobacter grow anaerobically using nitrate ammonification and fermentative processes: anaerobic adaptation of aerobic bacteria abundant in soil.members of the genus arthrobacter are usually regarded as obligate aerobic bacteria. the anaerobic growth and energy metabolism of two arthrobacter species were investigated. arthrobacter globiformis utilized both nitrate ammonification and lactate, acetate and ethanol producing fermentation processes for anaerobic growth. only nitrate supported anaerobic growth of arthrobacter nicotianae. anaerobically induced respiratory nitrate reductase activity was detected in both strains. neither of the t ...200312829291
a novel enzyme, d-3-hydroxyaspartate aldolase from paracoccus denitrificans ifo 13301: purification, characterization, and gene cloning.a novel enzyme, d-3-hydroxyaspartate aldolase (d-haa), catalyzing the conversion of d-3-hydroxyaspartate to glyoxylate plus glycine, was purified to homogeneity from paracoccus denitrificans ifo 13301. d-haa is strictly d-specific as to the alpha-position, whereas the enzyme does not distinguish between threo and erythro forms at the beta-position. in addition to d-3-hydroxyaspartate, the enzyme also acts on d-threonine, d-3-3,4-dihydroxyphenylserine, d-3-3,4-methylenedioxyphenylserine, and d-3- ...200312835921
characterization and molecular cloning of a novel enzyme, inorganic polyphosphate/atp-glucomannokinase, of arthrobacter sp. strain km.a bacterium exhibiting activities of several inorganic polyphosphate [poly(p)]- and atp-dependent kinases, including glucokinase, nad kinase, mannokinase, and fructokinase, was isolated, determined to belong to the genus arthrobacter, and designated arthrobacter sp. strain km. among the kinases, a novel enzyme responsible for the poly(p)- and atp-dependent mannokinase activities was purified 2,200-fold to homogeneity from a cell extract of the bacterium. the purified enzyme was a monomer with a ...200312839753
catabolism of arylboronic acids by arthrobacter nicotinovorans strain pba.arthrobacter sp. strain pba metabolized phenylboronic acid to phenol. the oxygen atom in phenol was shown to be derived from the atmosphere using (18)o(2). 1-naphthalene-, 2-naphthalene-, 3-cyanophenyl-, 2,5-fluorophenyl-, and 3-thiophene-boronic acids were also transformed to monooxygenated products. the oxygen atom in the product was bonded to the ring carbon atom originally bearing the boronic acid substituent with all the substrates tested.200312839810
isolation and characterization of psychrophiles producing cold-active beta-galactosidase.the present study was conducted to screen for psychrophilic micro-organisms that are able to hydrolyse lactose at low temperature, and to examine the cold-active beta-galactosidase produced by the isolated psychrophilic micro-organisms.200312859659
mode of action and antifungal properties of two cold-adapted chitinases.the mode of action of two chitinases from the antarctic arthrobacter sp. strain tad20 on n-acetyl-chitooligomers and chitin polymers has been elucidated. identification of the length of chitin oligomers following enzymatic hydrolysis was verified by using hplc-based analysis. it was observed that the length of the oligomer is important for enzyme action. the enzymes cannot effectively hydrolyze chitin oligomers with a degree of polymerization lower than four. archia is an endochitinase which hyd ...200312884086
purification and properties of a heat-stable inulin fructotransferase from arthrobacter inulin fructotransferase producing difructose dianhydride i (ec was purified from arthrobacter ureafaciens a51-1. it had maximum activity at ph 5.5 and 45 degrees c, and was stable up to 80 degrees c. this is the highest thermal stability for this enzyme reported to date. the molecular mass was estimated to be 38000 by sds-page, and 61000 by gel filtration. it was therefore estimated to be a dimer.200312889813
[xylose(glucose) isomerase reactivity of immobilized arthrobacter sp].the study of the xylose/glucose isomerase-containing arthrobacter sp. b-5 cells immobilized in cobalt hydroxide gel showed that immobilization increases the substrate affinity of the enzyme, its thermo- and ph-optima of action and stability, and makes unnecessary the addition of stabilizing cobalt ions to the isomerization medium.200312901016
the structure of 4-hydroxybenzoyl-coa thioesterase from arthrobacter sp. strain su.the 4-chlorobenzoyl-coa dehalogenation pathway in certain arthrobacter and pseudomonas bacterial species contains three enzymes: a ligase, a dehalogenase, and a thioesterase. here we describe the high resolution x-ray crystallographic structure of the 4-hydroxybenzoyl-coa thioesterase from arthrobacter sp. strain su. the tetrameric enzyme is a dimer of dimers with each subunit adopting the so-called "hot dog fold" composed of six strands of anti-parallel beta-sheet flanked on one side by a rathe ...200312907670
isolation of microorganisms for biological detoxification of lignocellulosic hydrolysates.acid pretreatment of lignocellulosic biomass releases furan and phenolic compounds, which are toxic to microorganisms used for subsequent fermentation. in this study, we isolated new microorganisms for depletion of inhibitors in lignocellulosic acid hydrolysates. a sequential enrichment strategy was used to isolate microorganisms from soil. selection was carried out in a defined mineral medium containing a mixture of ferulic acid (5 mm), 5-hydroxymethylfurfural (5-hmf, 15 mm), and furfural (20 m ...200412908085
channelling and formation of 'active' formaldehyde in dimethylglycine we report crystal structures of dimethylglycine oxidase (dmgo) from the bacterium arthrobacter globiformis, a bifunctional enzyme that catalyzes the oxidation of n,n-dimethyl glycine and the formation of 5,10-methylene tetrahydrofolate. the n-terminal region binds fad covalently and oxidizes dimethylglycine to a labile iminium intermediate. the c-terminal region binds tetrahydrofolate, comprises three domains arranged in a ring-like structure and is related to the t-protein of the glycine c ...200312912903
cold adaptation of a psychrophilic chitinase: a mutagenesis study.the gene encoding chitinase archib from the antarctic arthrobacter sp. tad20 has been expressed in escherichia coli and the recombinant enzyme purified to homogeneity. in an effort to engineer cold-adapted biocatalysts through rational redesign to operate at elevated temperatures, we performed several mutations aiming to increase the rigidity of the molecular edifice of the selected psychrophilic chitinase. the mutations were designed on the basis of a homology-based three-dimensional model of t ...200312915727
ph and deuterium kinetic isotope effects studies on the oxidation of choline to betaine-aldehyde catalyzed by choline oxidase.the fad-dependent choline oxidase catalyzes the four-electron oxidation of choline to glycine-betaine, with betaine-aldehyde as intermediate. the enzyme is capable of accepting either choline or betaine-aldehyde as a substrate, allowing the investigation of the reaction mechanism for both the conversion of choline to betaine-aldehyde and of betaine-aldehyde to glycine-betaine. in the present study, ph and deuterium kinetic isotope effects with [1,2-2h(4)]-choline were used to study the mechanism ...200312922164
crystallization and preliminary x-ray analysis of inorganic polyphosphate/atp-glucomannokinase from arthrobacter sp. strain km.inorganic polyphosphate [poly(p)]/atp-glucomannokinase from arthrobacter sp. strain km phosphorylates glucose and mannose, utilizing both atp and poly(p) as phosphoryl donors. the enzyme was overexpressed in escherichia coli, purified and crystallized by means of the hanging-drop vapour-diffusion method with ammonium sulfate as the precipitant. the crystals were orthorhombic and belonged to space group p2(1)2(1)2(1), with unit-cell parameters a = 66.2, b = 83.7, c = 103.8 a. assuming two molecul ...200312925806
biochemical characterization of a beta-galactosidase with a low temperature optimum obtained from an antarctic arthrobacter isolate.a psychrophilic gram-positive isolate was obtained from antarctic dry valley soil. it utilized lactose, had a rod-coccus cycle, and contained lysine as the diamino acid in its cell wall. consistent with these physiological traits, the 16s ribosomal dna sequence showed that it was phylogenetically related to other arthrobacter species. a gene (bgas) encoding a family 2 beta-galactosidase was cloned from this organism into an escherichia coli host. preliminary results showed that the enzyme was co ...200312949099
inhibition of beta-fructofuranosidases and alpha-glucosidases by synthetic thio-fructofuranoside.a synthetic beta-thio-fructofuranoside of mercaptoethanol inhibited not only beta-fructofuranosidases but also alpha-glucosidases. the compound was hardly hydrolyzed by the glycosidases. the thio-fructoside competitively inhibited beta-fructofuranosidases from aspergillus niger, candida sp., and saccharomyces cerevisiae, but not arthrobacter beta-fructofuranosidase at all. sucrase activity of rat intestinal sucrase/isomaltase complex was also suppressed in the presence of the thio-fructoside. th ...200312951505
arthrobacter koreensis sp. nov., a novel alkalitolerant bacterium from soil.two arthrobacter strains, ca15-8(t) and ca15-9, were isolated from soil, under alkali conditions, and characterized phenotypically, chemotaxonomically and genetically. these alkalitolerant organisms grew over a wide ph range (ph 7.0-12.0), with an optimum at ph 7.0-8.0. the mean g+c content of the dna of these strains was 63+/-2 mol%. the strains contained mk-8(h(2)) and mk-9(h(2)) as the main respiratory quinones. the cell-wall peptidoglycan was lys-thr-ala(2) and the whole-cell sugar was rhamn ...200313130006
observations on the morphogenesis of arthrobacter citreus, spec nov. 195413143001
morphological comparison of some arthrobacter species. 195813536906
studies on the metabolic function of the ferrichrome compounds. 196113689181
[on the decomposition of nicotine by bacterial enzymes. ii. isolation and characterization of a nicotine-splitting soil bacterium]. 196113696685
[on the decomposition of nicotine by bacterial enzymes. iv. l-6-hydroxynicotine as the first intermediate product]. 196113721110
[on the degradation of nicotine by bacterial enzymes]. 196013721111
[on the decomposition of nicotine by bacterial enzymes. iii. metabolic studies on cell free extracts]. 196113721112
arthrobacter atrocyaneus, n. sp., and its blue pigment. 196013856051
influence of a bacterial cell extract upon the morphogenesis of arthrobacter ureafaciens.blankenship, l. c. (university of maryland, college park) and r. n. doetsch. influence of a bacterial cell extract upon the morphogenesis of arthrobacter ureafaciens. j. bacteriol. 82:882-888. 1961-the effect of a bacterial extract on alleviating the abnormal morphological appearance of arthrobacter ureafaciens when cultivated in thiotone broth is described. physical and chemical analyses of this extract revealed that the mineral components therein were largely responsible for the observed effec ...196113869842
bacterial iron metabolism: investigations on the mechanism of ferrichrome function. 196213874972
growth interactions of arthrobacter globiformis and pseudomonas sp. in relation to the rhizosphere effect. 196113878001
on the biotin requirement of arthrobacter globiformis. 196213878002
chemical composition and antigenic structure of cell walls of corynebacterium, mycobacterium, nocardia, actinomyces and arthrobacter. 196213882624
a ferrichrome-requiring arthrobacter which decomposes puromycin aminonucleoside. 196213901475
decomposition of puromycin aminoncleoside by arthrobacter flavescens. 196213901476
[6-hydroxy-pyridyl-(3)]-ketone]. 196113901804
nicotine oxidation by arthrobacter. 196113901850
phosphorylation of shikimic acid by ultrasonic extracts of micro-organisms. 196213944994
metabolism of glycerol by an arthrobacter species. 196213977220
glutamic acid production by arthrobacter globiformis. 196313996592
comparative carbohydrate catabolism in arthrobacter. 196214003016
a preliminary report on the fine structure of a bacteriophage of arthrobacter polychromogenes schippers-lammertse, muysers et klatser-oedekerk. 196314024588
production of a gibberellin-like substance by arthrobacter globiformis. 196214031348
on the origin of v-forms in arthrobacter atrocyaneus. 196214039490
investigations on the action of the iron-containing growth factors, sideramines; and iron-containing antibiotics, sideromycins. 196314045511
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