Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| transfer of man9glcnac to l-fucose by endo-beta-n-acetylglucosaminidase from arthrobacter protophormiae. | we have reported that transglycosylation activity of endo-beta-n-acetylglucosaminidase from arthrobacter protophormiae (endo-a) can be enhanced to near completion using glcnac as an acceptor in a medium containing 30% acetone (fan j-q, takegawa k, iwahara s, kondo a, kato i, abeygunawardana c, lee yc (1995) j biol chem 270: 17723-29). in this paper, we found that the endo-a can also transfer an oligosaccharide, man9glcnac, to l-fuc using man9glcnac2asn as donor substrate in a medium containing 3 ... | 1996 | 8872122 |
| [microflora of the air in sawmills as a potential occupational hazard: concentration and composition of microflora and immunologic reactivity of workers to microbial aeroallergens]. | microbiologial studies of the air and allergological examinations of the workers were performed in two sawmills processing deciduous wood (mainly oak) and in one sawmill processing coniferous wood (mainly pine). the concentration of microorganisms in the air was of the order 10(3)-10(4) cfu/m3. the most common organisms were corynebacteria (arthrobacter, corynebacterium, brevibacterium, microbacterium), spore-forming bacilli (bacillus), gram-negative bacteria (rahnella) and filamentous fungi (as ... | 1996 | 9190234 |
| [microflora of the in furniture factors as a potential occupational hazard: concentration and composition of microflora and immunologic reactivity of workers to microbial aeroallergens]. | microbiologial studies of the air were performed in two furniture factories. the concentration of microorganisms in the air was low, being of the order 10(3) cfu/m3. the most common organisms were corynebacteria (arthrobacter, corynebacterium, brevibacterium, microbacterium) and fungi (aspergillus fumigatus, rhodotorula rubra). some of the species found in this environment possess known allergenic properties. allergological examinations of the workers with environmental aeroallergens have been p ... | 1996 | 9190236 |
| isolation of arthrobacter spp. from clinical specimens and description of arthrobacter cumminsii sp. nov. and arthrobacter woluwensis sp. nov. | arthrobacter spp. are very widely distributed in the environment (e.g., soil) but have not been described as causing disease in humans. over a 6-year period, two reference laboratories isolated or received 11 strains which were eventually identified as belonging to the genus arthrobacter. these strains had been initially identified as centers for disease control and prevention coryneform group b-1 and b-3 bacteria (whitishgrayish colonies of 2 mm or greater in diameter after 24 h of incubation, ... | 1996 | 8880479 |
| cloning and sequencing of trehalose biosynthesis genes from arthrobacter sp. q36. | a 5.1-kbp genomic dna fragment was cloned from trehalose-producing bacterium arthrobacter sp. strain q36. sequence analysis of the dna fragment revealed two open reading frames of 2325 and 1794 bp, encoding maltooligosyltrehalose synthase (trey) and maltooligosyltrehalose trehalohydrolase (trez), respectively. the 3' end of trey overlaps with the 5' end of trez by one nucleotide, and it is suggested that treyz constitutes and operon. the deduced amino acid sequences of both enzymes have several ... | 1996 | 8605217 |
| genetic characterization of site-specific integration functions of phi aau2 infecting "arthrobacter aureus" c70. | all the essential genetic determinants for site-specific integration of corynephage phi aau2 are contained within a 1,756-bp dna fragment, carried on the integrative plasmid p5510, and are shown to be functional in escherichia coli. one open reading frame, orf4, encoding a protein of 266 amino acids was shown to represent the phi aau2 integrase. the nucleotide sequence of the phi aau2 attachment site, attp, and the attb, attl, and attr sequences in the host "arthrobacter aureus" c70 were determi ... | 1996 | 8606175 |
| sarcosine oxidase contains a novel covalently bound fmn. | sarcosine oxidase from corynebacterium sp. p-1 is a heterotetrameric protein containing three different enzymes: noncovalent fad, noncovalent nad+, and covalently bound flavin which is released as 8 alpha-(n3-histidyl)riboflavin upon complete hydrolysis of the protein. the following results show that the covalent flavin is not at the fad level, as previously proposed, but it is rather as 8 alpha-(n3- histidyl)fmn coenzyme. first, no amp is released when the protein moiety is treated with phospho ... | 1996 | 8611516 |
| biodegradation of dimethylsilanediol in soils. | the biodegradation potential of [14c]dimethylsilanediol, the monomer unit of polydimethylsiloxane, in soils was investigated. dimethylsilanediol was found to be biodegraded in all of the tested soils, as monitored by the production of 14co2. when 2-propanol was added to the soil as a carbon source in addition to [14c]dimethylsilanediol, the production of 14co2 increased. a method for the selection of primary substrates that support cometabolic degradation of a target compound was developed. by t ... | 1996 | 8953708 |
| cloning of the penicillium minioluteum gene encoding dextranase and its expression in pichia pastoris. | the dex gene encoding an extracellular dextranase was isolated from the genomic dna library of penicillium minioluteum by hybridization using the dextranase cdna as a probe. comparison of the gene and cdna sequences revealed that the dex gene does not contain introns. amino acid sequences comparison of p. minioluteum dextranase with other reported dextranases reveals a significant homology (29% identity) with a dextranase from arthrobacter sp. cb-8. the dex gene fragment encoding a mature protei ... | 1996 | 8905923 |
| 2,4-dioxygenases catalyzing n-heterocyclic-ring cleavage and formation of carbon monoxide. purification and some properties of 1h-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase from arthrobacter sp. rü61a and comparison with 1h-3-hydroxy-4-oxoquinoline 2,4-dioxygenase from pseudomonas putida 33/1. | 1h-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase (meqdo) was purified from quinaldine-grown arthrobacter sp. rü61a. it was enriched 59-fold in a yield of 22%, and its properties were compared with 1h-3-hydroxy-4-oxoquinoline 2,4-dioxygenase (qdo) purified from pseudomonas putida 33/1. the enzyme-catalyzed conversions were performed in an (18o)o2/(16o)o2 atmosphere. two oxygen atoms of either (18o)o2 or (16o)o2 were incorporated at c2 and c4 of the respective substrates, indicating that these unusual ... | 1996 | 8856057 |
| cloning and sequencing of a dextranase-encoding cdna from penicillium minioluteum. | a cdna from penicillium minioluteum hi-4 encoding a dextranase (1,6-alpha-glucan hydrolase, ec 3.2.1.11) was isolated and characterized. cdna clones corresponding to genes expressed in dextran-induced cultures were identified by differential hybridization. southern hybridization and restriction mapping analysis of selected clones revealed four different groups of cdnas. the dextranase cdna was identified after expressing a cdna fragment from each of the isolated groups of cdna clones in the esch ... | 1996 | 8837470 |
| microbial biosensor for free fatty acids using an oxygen electrode based on thick film technology. | a microbial biosensor based on thick film technology was developed. the microorganisms, arthrobacter nicotianae, were immobilized in ca-alginate directly on the electrode surface. for the stability of the calcium alginate gel the addition of 0.5 mm cacl2 to the assay buffer was necessary. the respiratory activity of the microorganisms was monitored by oxygen consumption at -600 mv vs. ag/agcl reference electrode. the sensor was used in a batch system and was applied to the determination of free ... | 1996 | 8828165 |
| ym-30059, a novel quinolone antibiotic produced by arthrobacter sp. | 1996 | 8823519 | |
| assessment of the biodegradation potential of psychrotrophic microorganisms. | bioremediation of polluted temperate and cold temperature environments may require the activity of psychrotrophic bacteria, because their low temperature growth range parallels the ambient temperatures encountered in these environments. in the present study, 135 psychrotrophic microorganisms isolated from a variety of ecosystems in canada were examined for their ability to mineralize 14c-labelled toluene, naphthalene, dodecane, hexadecane, 2-chlorobiphenyl, and pentachlorophenol. a number of the ... | 1996 | 8742353 |
| effects of copper-chrome-arsenate (cca) components on pcp degradation by arthrobacter strain atcc 33790. | 1996 | 8661907 | |
| manganese(ii)-dependent extradiol-cleaving catechol dioxygenase from arthrobacter globiformis cm-2. | a manganese-dependent 3,4-dihydroxyphenylactate 2,3-dioxygenase from arthrobacter globiformis strain cm-2 (mndd) cloned in escherichia coli has been purified to homogeneity. sedimentation equilibrium analysis indicates an alpha 4 homotetrameric holoenzyme structure (4 x 38,861 da). steady-state kinetic analysis of mndd with a variety of substrates and inhibitors yields very similar relative rates to the known fe(ii)- and mn(ii)-dependent 3,4-dihydroxyphenylacetate 2,3-dioxygenases from pseudomon ... | 1996 | 8555170 |
| quinaldine 4-oxidase from arthrobacter sp. rü61a, a versatile procaryotic molybdenum-containing hydroxylase active towards n-containing heterocyclic compounds and aromatic aldehydes. | quinaldine 4-oxidase from arthrobacter sp. rü61a, an inducible molybdenum-containing hydroxylase, was purified to homogeneity by an optimized five-step procedure. molecular oxygen is proposed as physiological electron acceptor. electrons are also transferred to artificial electron acceptors with e'o > -8 mv. the molybdo-iron/sulfur flavoprotein regiospecifically attacks its n-heterocyclic substrates: isoquinoline and phthalazine are hydroxylated adjacent to the n-heteroatom at cl, whereas quinal ... | 1996 | 8617260 |
| distribution of thiols in microorganisms: mycothiol is a major thiol in most actinomycetes. | mycothiol [2-(n-acetylcysteinyl)amido-2-deoxy-alpha-d-glucopyranosyl- (1-->1)-myo-inositol] (msh) has recently been identified as a major thiol in a number of actinomycetes (s. sakuda, z.-y. zhou, and y. yamada, biosci. biotech. biochem. 58:1347-1348, 1994; h. s. c. spies and d. j. steenkamp, eur. j. biochem. 224:203-213, 1994; and g. l. newton, c. a. bewley, t. j. dwyer, r. horn, y. aharonowitz, g. cohen, j. davies, d. j. faulkner, and r. c. fahey, eur. j. biochem. 230:821-825, 1995). since thi ... | 1996 | 8606174 |
| crystallographic and fluorescence studies of ligand binding to n-carbamoylsarcosine amidohydrolase from arthrobacter sp. | crystal structures of n-carbamoylsarcosine amidohydrolase (cshase; ec 3.5.1.59) have been analyzed by x-ray diffraction methods with two different inhibitors bound to the active site at 2.28 a and 2.37 a resolution. the catalytic center of the enzyme could be identified on the basis of these structures. the four substrate binding sites are situated at the intersubunit interfaces of the compact dimers ab and cd of the homotetrameric enzyme. both inhibitors inactivate the enzyme irreversibly throu ... | 1996 | 8913306 |
| nucleotide sequence and taxonomical distribution of the bacteriocin gene lin cloned from brevibacterium linens m18. | linocin m18 is an antilisterial bacteriocin produced by the red smear cheese bacterium brevibacterium linens m18. oligonucleotide probes based on the n-terminal amino acid sequence were used to locate its single copy gene, lin, on the chromosomal dna. the amino acid composition, n-terminal sequence, and molecular mass derived from the nucleotide sequence of an open reading frame of 798 nucleotides coding for 266 amino acids found on a 3-kb bamhi restriction fragment correspond closely to those o ... | 1996 | 8919789 |
| endophthalmitis involving an arthrobacter-like organism following intraocular lens implantation. | 1996 | 8922827 | |
| the species composition of oligotrophic bacterial communities of lake balaton--a numerical analysis. | from among the 565 aerobic and facultatively anaerobic, oligotrophic bacterial isolated obtained from the open water region of the balatonfüred-bay, 58 representative strains were selected for detailed taxonomic-physiological studies and numerical analyses as well as simultaneous comparisons with authentic bacterial strains isolated earlier by langó (1987) from different water habitat of the lake balaton. on the basis of 186 ceded differential diagnostic characteristics involved in these analyse ... | 1996 | 9147724 |
| biodegradation of 4-chlorophenol via a hydroquinone pathway by arthrobacter ureafaciens cpr706. | a newly isolated arthrobacter ureafaciens, strain cpr706, could degrade 4-chlorophenol via a new pathway, in which the chloro-substituent was eliminated in the first step and hydroquinone was produced as a transient intermediate. strain cpr706 exhibited much higher substrate tolerance and degradation rate than other strains that degraded 4-chlorophenol by the hydroxylation at the second carbon position to form chlorocatechol. strain cpr706 could also degrade other para-substituted phenols (4-nit ... | 1996 | 8931337 |
| cloning and sequencing of a cluster of genes encoding novel enzymes of trehalose biosynthesis from thermophilic archaebacterium sulfolobus acidocaldarius. | trehalose biosynthesis genes, trez, trex and trey, encoding maltooligosyltrehalose trehalohydrolase (trez), glycogen debranching enzyme (trex), and maltooligosyltrehalose synthase (trey) have been cloned from the thermophilic archaebacterium sulfolobus acidocaldarius atcc33909. the amino-acid sequences deduced from trez, trex and trey are composed of 556, 713 and 720 amino-acid residues, respectively. trez and trey are 33-40% homologous to the corresponding enzymes from arthrobacter sp. q36. we ... | 1996 | 8980629 |
| flagellar proteins of motile spores of actinomycetes. | flagella of some of the actinoplanete genera were purified and the molecular sizes of their flagellin subunits compared by sds-page analysis to flagellins of cells of other bacteria. several species of actinoplanes have a major flagellar protein of subunit sizes of 42-43 kda and a lesser amount of a second protein, possibly a minor flagellin subunit, of 60 kda. the flagellar protein sizes of other actinoplanetes ranged from 32-43 kda (major) and 48-58 kda (minor). antibodies formed against the 4 ... | 1996 | 8987497 |
| biodegradability of oxidized poly(vinyl alcohol). | poly(vinyl alcohol) dehydrogenase (pvadh) purified from pseudomonas sp. 113p3 catalyzed an oxidation of poly(vinyl alcohol) (pva) in the presence of pyrroloquinoline quinone (pqq) to give a beta-diketone structure on pva. although pvadh oxidized not only enzymatically oxidized pva but also chemically oxidized pva, pva-degrading microorganisms, pseudomonas sp. 113p3 and arthrobacter tumescens sp. 52-1 grew on the enzymatically oxidized pva, but not on the chemically oxidized pva. this suggests th ... | 1996 | 8987864 |
| metabolism of nitrate esters by a consortium of two bacteria. | the products of condensation of organic alcohols and nitric acid are nitrate esters with the general structure c-o-no2. these products are widely employed as vasodilators and explosives, and are true xenobiotic compounds, as they do not occur in nature. we have isolated and characterized a consortium of two microorganisms, arthrobacter ilicis and agrobacterium radiobacter, that mineralized recalcitrant ethylene glycol dinitrate. the arthrobacter strain was the actual degrading microorganism, alt ... | 1996 | 9630893 |
| hydroxylamine oxidation in heterotrophic nitrate-reducing soil bacteria and purification of a hydroxylamine-cytochrome c oxidoreductase from a pseudomonas species. | hydroxylamine oxidation was measured in four recently isolated heterotrophic nitrate-reducing bacteria belonging to the genera pseudomonas, moraxella, arthrobacter and aeromonas. a hydroxylamine-cytochrome c oxidoreductase activity was detected in periplasmic fractions of the pseudomonas and aeromonas spp. and in total soluble fractions of the arthrobacter sp. a monomeric 19-kda non-haem iron hydroxylamine-cytochrome c oxidoreductase was purified from the pseudomonas species and shown to be simi ... | 1996 | 9082922 |
| lectins binding on human sperm surface increase membrane permeability and stimulate acrosomal exocytosis. | cross-linked complexes formed between certain lectins and their specific multivalent carbohydrates and glycoconjugates on the sperm surface were studied for their ability to modify sperm membrane permeability and to induce the acrosome reaction. wheat germ agglutinin (wga), concanavalin a (con a) and peanut agglutinin (pna) increased the proportions of human spermatozoa permeable to the impermeable propidium iodide (31.9 compared with 13.8%, 38.4 compared with 18.4% and 72.7 compared with 18.9% ... | 1996 | 9239678 |
| capa, a cspa-like gene that encodes a cold acclimation protein in the psychrotrophic bacterium arthrobacter globiformis si55. | by use of arthrobacter globiformis si55, a psychrotrophic bacterium capable of growth between -5 and +32 degrees c, we cloned and sequenced capa, a gene homologous to cspa encoding the major cold shock protein in escherichia coli. the deduced protein sequence has a high level of identity with the sequences of other cspa-related proteins from various sources, and no particular residue or domain that could be specific to cold-adapted microorganisms emerged. we show that capa was produced very rapi ... | 1997 | 9294420 |
| molecular cloning of levan fructotransferase gene from arthrobacter nicotinovorans gs-9 and its expression in escherichia coli. | the gene encoding an extracellular levan fructotransferase, designated the lft gene, was cloned from the genomic dna of arthrobacter nicotinovorans gs-9, and expressed in escherichia coli. it was found that a single open reading frame consisted of 1554 base pairs that encoded a polypeptide composed of a signal peptide of 33 amino acids and a mature protein of 484 amino acids (m(r) 53,152), and it was also found that a putative ribosome-binding site was present in the upstream from the orf. the p ... | 1997 | 9438987 |
| plant compounds that induce polychlorinated biphenyl biodegradation by arthrobacter sp. strain b1b. | plant compounds that induced arthrobacter sp. strain b1b to cometabolize polychlorinated biphenyls (pcbs) were identified by a screening assay based on the formation of a 4,4'-dichlorobiphenyl ring fission product. a chemical component of spearmint (mentha spicata), l-carvone, induced arthrobacter sp. strain b1b to cometabolize aroclor 1242, resulting in significant degradation of 26 peaks in the mixture, including selected tetra- and pentachlorobiphenyls. evidence for pcb biodegradation include ... | 1997 | 9143124 |
| role of electrostatics at the catalytic metal binding site in xylose isomerase action: ca(2+)-inhibition and metal competence in the double mutant d254e/d256e. | the catalytic metal binding site of xylose isomerase from arthrobacter b3728 was modified by protein engineering to diminish the inhibitory effect of ca2+ and to study the competence of metals on catalysis. to exclude ca2+ from site 2 a double mutant d254e/d256e was designed with reduced space available for binding. in order to elucidate structural consequences of the mutation the binary complex of the mutant with mg2+ as well as ternary complexes with bivalent metal ions and the open-chain inhi ... | 1997 | 9188736 |
| structural studies on a sulfated polysaccharide from an arthrobacter sp. by nmr spectroscopy and methylation analysis. | structural characterization of a sulfated polysaccharide peptidoglycan complex (sp-pg) from an arthrobacter sp. was performed by nmr spectroscopy and methylation analysis. in order to simplify the analyses, the desulfated sp-pg was used. nmr spectroscopy revealed the presence of a trisaccharide repeating unit and a disaccharide repeating unit. the trisaccharide unit was composed of two galactofuranosides and one glucopyranoside, and the disaccharide unit was of two galactopyranosides, as shown b ... | 1997 | 9581278 |
| 16s rrna gene sequence of rubrobacter radiotolerans and its phylogenetic alignment with members of the genus arthrobacter, gram-positive bacteria, and members of the family deinococcaceae. | the nearly complete sequence of the 16s rrna gene of an extremely highly radiotolerant bacterium, rubrobacter radiotolerans (reclassified from arthrobacter radiotolerans based on chemical characteristics), was determined by pcr amplification of the genomic dna followed by cloning of the amplified gene and sequencing by the dideoxynucleotide method. the sequence was aligned with the sequences of members of the genus arthrobacter and also with the sequences of representatives of the gram-positive ... | 1997 | 9226900 |
| leukosialin (cd43) is proteolytically cleaved from stimulated hmc-1 cells. | leukosialin (cd43), the major sialoprotein on circulating leukocytes, has been previously described to be down-regulated on neutrophils following activation with phorbol myristate acetate (pma). the other single cells previously examined, blood lymphocytes, do not down-regulate cd43 when stimulated by pma. recently, we have characterized leukosialin on the human mast cell line hmc-1 and observed that leukosialin is down-regulated after stimulation with pma. in the present study, we have investig ... | 1997 | 9241533 |
| genetic identification of members of the genus corynebacterium at genus and species levels with 16s rdna-targeted probes. | 16s rrna gene-targeted probes were designed for the identification of corynebacteria at the genus and species levels. the genus-specific probe hybridized all clinically important members of the genus corynebacterium and could distinguish them from other coryneform bacteria and phylogenetically related high g+c% gram-positive bacteria, including actinomyces, rhodococcus, gordona, nocardia, streptomyces, brevibacterium and mycobacterium. the species-specific probes for c. jeikeium and c. diphtheri ... | 1997 | 9251056 |
| tissue-specific heterogeneity in alpha-dystroglycan sialoglycosylation. skeletal muscle alpha-dystroglycan is a latent receptor for vicia villosa agglutinin b4 masked by sialic acid modification. | because the polypeptide core of alpha-dystroglycan is encoded by a single gene, the difference in apparent molecular mass between alpha-dystroglycans expressed in various tissues is presumably due to differential glycosylation. however, little is presently known about the tissue-specific differences in alpha-dystroglycan glycosylation and whether these modifications may confer functional variability to alpha-dystroglycan. we recently observed that laminin-1 binding to skeletal muscle alpha-dystr ... | 1997 | 9268382 |
| topaquinone-dependent amine oxidases: identification of reaction intermediates by raman spectroscopy. | resonance raman (rr) spectroscopy has proven to be an excellent technique for providing structural information about the 2,4, 5-trihydroxyphenylalaninequinone (tpq) cofactor and for identifying the source of oxygen atoms during the posttranslational synthesis of the cofactor. through specific labeling of the c2, c4, and c5 oxygens of tpq in phenylethylamine oxidase (peao) from arthrobacter globiformis, we have identified the c=o stretch of the c5 carbonyl at 1683 cm-1 (-27 in 18o) and the c=o st ... | 1997 | 9298968 |
| phylogenetic characterization of bacteria in the subsurface microbial culture collection. | the subsurface microbial culture collection (smcc) was established by the u.s. dept. of energy (doe) and contains nearly 10,000 strains of microorganisms (mostly bacteria) isolated from terrestrial subsurface environments. selected groups of bacterial isolates from three sample sites situated above geochemically and hydrologically different subsurface environments have been characterized by phylogenetic analysis of 16s ribosomal rna (rrna) gene nucleotide sequences. among these isolates were mem ... | 1997 | 9299704 |
| detection of heavy metal ion resistance genes in gram-positive and gram-negative bacteria isolated from a lead-contaminated site. | resistance to a range of heavy metal ions was determined for lead-resistant and other bacteria which had been isolated from a battery-manufacturing site contaminated with high concentration of lead. several gram-positive (belonging to the genera arthrobacter and corynebacterium) and gram-negative (alcaligenes species) isolates were resistant to lead, mercury, cadmium, cobalt, zinc and copper, although the levels of resistance to the different metal ions were specific for each isolate. polymerase ... | 1997 | 9342884 |
| isolation and characterization of d-threonine aldolase, a pyridoxal-5'-phosphate-dependent enzyme from arthrobacter sp. dk-38. | d-threonine aldolase is an enzyme that catalyzes the cleavage of d-threonine into glycine and acetaldehyde. its activity was found in several genera of bacteria such as arthrobacter, alcaligenes, xanthomonas, and pseudomonas, but not in yeasts or fungi. the enzyme was purified to homogeneity from one strain, arthrobacter sp. dk-38. the enzyme appeared to consist of a single polypeptide chain with an apparent molecular mass of 51 kda. this enzyme, as well as l-threonine aldolase, requires pyridox ... | 1997 | 9346293 |
| isolation of alu1-p gene encoding a protein with aluminum tolerance activity from arthrobacter viscosus. | we isolated a dna fragment (alu1-p) encoding a protein with an activity of aluminum tolerance from an al tolerant soil microorganism, arthrobacter viscosus. this microorganism was isolated from acidic tea field soils. the cloned dna is composed of 1090 nucleotides, which has one open-reading frame without any stop codon. however, when the dna fragment was transferred into escherichia coli, a microorganism susceptible to al toxicity, it endowed e. coli with al tolerance. the deduced amino acid se ... | 1997 | 9367855 |
| crystal structures of the copper-containing amine oxidase from arthrobacter globiformis in the holo and apo forms: implications for the biogenesis of topaquinone. | the crystal structures of the copper enzyme phenylethylamine oxidase from the gram-positive bacterium arthrobacter globiformis (agao) have been determined and refined for three forms of the enzyme: the holoenzyme in its active form (at 2.2 a resolution), the holoenzyme in an inactive form (at 2.8 a resolution), and the apoenzyme (at 2.2 a resolution). the holoenzyme has a topaquinone (tpq) cofactor formed from the apoenzyme by the post-translational modification of a tyrosine residue in the pres ... | 1997 | 9405045 |
| [dynamics of bacteria from the phyllosphere and leaves of soy (glycine max l. merrill) in field conditions]. | the surfaces of aerial plant provide a habitat for epiphitic microorganisms many of which are capable of influencing the growth of pathogens. in this study, fluctuation of bacterial population occurring in different growth stages of soybean leaves (glycine max l merrill) was examined using the dilution plate method. three culture media were applied; invariably, the numbers of bacteria increased with increasing plant age. the total number of bacteria living on the detached leaves were ten times h ... | 1997 | 9411487 |
| biotreatment of ammonia from air by an immobilized arthrobacter oxydans ch8 biofilter. | a heterotrophic arthrobacter oxydans ch8 that was capable of removing nh3 from nh3 containing gas was isolated from livestock farming wastewater. the a. oxydans ch8 was immobilized with calcium alginate packed into filter column. metered nh3-containing gas was partially humidified and passed through the glass column. extensive tests including the removal characteristics, the removal efficiencies, and the metabolic products of nh3 by a. oxydans ch8 were conducted. additionally, the operation crit ... | 1997 | 9413137 |
| molybdate-uptake genes and molybdopterin-biosynthesis genes on a bacterial plasmid--characterization of moea as a filament-forming protein with adenosinetriphosphatase activity. | a gene cluster consisting of homologs to escherichia coli moaa, moea, moac and moae, which encode enzymes involved in the biosynthesis of molybdopterin cofactor (moco), and to moda, modb and modc, which encode a high-affinity molybdate transporter, were identified on pao1 of arthrobacter nicotinovorans near genes of molybdopterin-dependent enzymes involved in nicotine degradation. this gene arrangement suggests a coordinated expression of the moco-dependent and the moco-biosynthesis genes and sh ... | 1997 | 9428706 |
| purification and characterization of polyamine aminotransferase of arthrobacter sp. tmp-1. | polyamine aminotransferase of arthrobacter sp. tmp-1 was induced by 1,3-diaminopropane (dap), n-3-aminopropyl-1,3-diaminopropane (norspermidine), spermidine, and spermine, but not by putrescine. the enzyme was purified to homogeneity. its molecular weight and subunit size were 129,000 and 64,000, respectively. its absorption spectrum had maxima at 280 and 420 nm and a shoulder at about 350 nm, and changes were observed upon the addition of dap, putrescine, and sodium borohydride. the spectrum an ... | 1997 | 9348081 |
| action of polyamine aminotransferase on norspermidine. | the norspermidine-pyruvate reaction catalyzed by polyamine aminotransferase from arthrobacter sp. tmp-1 formed n-3-aminopropyl-3-aminopropionaldehyde (apapal), l-alanine, 1,3-diaminopropane (dap), allylamine, and acrolein, and the relative rates of formation of the latter four products were 24, 3.3, 2.3, and 1.2%, respectively, of the rate of the dap-pyruvate transamination. the identification of apapal was done by 13c-nmr after it had been enzymatically oxidized to n-3-aminopropyl-beta-alanine ... | 1997 | 9348082 |
| a genetic and functional analysis of the unusually large variable region in the m.alui dna-(cytosine c5)-methyltransferase. | the m.alui dna-(cytosine c5)-methyltransferase (5mc methylase) acts on the sequence 5'-agct-3'. the amino acid sequences of known 5mc methylases contain ten conserved motifs, with a variable region between motifs viii and ix that contains one or more "target-recognizing domains" (trds) responsible for dna sequence specificity. monospecific 5mc methylases are believed to have only one trd, while multispecific 5mc methylases have as many as five. m.alui has the second-largest variable region of al ... | 1997 | 9439564 |
| the action in vivo of glycine betaine in enhancement of tolerance of synechococcus sp. strain pcc 7942 to low temperature. | the cyanobacterium synechococcus sp. strain pcc 7942 was transformed with the coda gene for choline oxidase from arthrobacter globiformis under the control of a constitutive promoter. this transformation allowed the cyanobacterial cells to accumulate glycine betaine at 60 to 80 mm in the cytoplasm. the transformed cells could grow at 20 degrees c, the temperature at which the growth of control cells was markedly suppressed. photosynthesis of the transformed cells at 20 degrees c was more toleran ... | 1997 | 8990284 |
| structures of sialylated o-linked oligosaccharides of bovine peripheral nerve alpha-dystroglycan. the role of a novel o-mannosyl-type oligosaccharide in the binding of alpha-dystroglycan with laminin. | alpha-dystroglycan is a heavily glycosylated protein, which is localized on the schwann cell membrane as well as the sarcolemma, and links the transmembrane protein beta-dystroglycan to laminin in the extracellular matrix. we have shown previously that sialidase treatment, but not n-glycanase treatment, of bovine peripheral nerve alpha-dystroglycan greatly reduces its binding activity to laminin, suggesting that the sialic acid of o-glycosidically-linked oligosaccharides may be essential for thi ... | 1997 | 8999917 |
| molecular cloning of an inulin fructotransferase (depolymerizing) gene from arthrobacter sp. h65-7 and its expression in escherichia coli. | the gene encoding an extracellular inulin fructotransferase (depolymerizing) (inulase ii) (ec 2.4.1.93), designated ift gene, was cloned from the genomic dna of arthrobacter sp. h65-7, and expressed in escherichia coli for the first time. sequence analysis showed a single open reading frame consisting of 1314 base pairs that encoded a signal peptide of 32 amino acids and a mature protein of 405 amino acids. the primary structure showed a homology of 49.8% with that of the inulin fructotransferas ... | 1997 | 9028036 |
| biodegradation of methyl t-butyl ether by pure bacterial cultures. | three pure bacterial cultures degrading methyl t-butyl ether (mtbe) were isolated from activated sludge and fruit of the gingko tree. they have been classified as belonging to the genuses methylobacterium, rhodococcus, and arthrobacter. these cultures degraded 60 ppm mtbe in 1-2 weeks of incubation at 23-25 degrees c. the growth of the isolates on mtbe as sole carbon source is very slow compared with growth on nutrient-rich medium. uniformly-labeled [14c]mtbe was used to determine 14co2 evolutio ... | 1997 | 9035411 |
| mechanistic studies of topa quinone biogenesis in phenylethylamine oxidase. | an alternative purification for apophenylethylamine oxidase from arthrobacter globiformis has been developed, which avoids the use of possible contaminants that may interfere with the topa quinone (tpq) self-processing reaction. the binding of cu(ii) and the kinetics of tpq formation in these enzyme preparations have been reinvestigated. our results show that cu(ii) is not significantly reduced when added to the apoprotein under anaerobic conditions. the cu(ii) epr and circular dichroism spectra ... | 1997 | 9047291 |
| rapid physiological characterization of microorganisms by biosensor technique. | eleven microorganisms, arxula adeninivorans ls3, candida boidinii dsm 70034, candida lactis-condensi dsm 70635, pichia jadinii dsm 2361, pichia minuta dsm 7018, kluyveromyces lactis dsm 4394, pseudomonas putida dsm 50026, alcaligenes sp. dsm 30002, arthrobacter nicotianae dsm 20123 as well as issatchenkia orientalis dsm 70077 and rhodococcus erythropolis dsm 311 were characterized by the sensor technique by injection of 30 different substrates and substrate mixtures. the obtained data which are ... | 1997 | 9352658 |
| manganese(ii) active site mutants of 3,4-dihydroxyphenylacetate 2,3-dioxygenase from arthrobacter globiformis strain cm-2. | whereas all other members of the extradiol-cleaving catechol dioxygenase family are iron-dependent, the 3,4-dihydroxyphenylacetate 2,3-dioxygenase (mndd) from arthrobacter globiformis cm-2 is dependent on manganese for catalytic activity. recently, the endogenous iron ligands of one family member, the 2,3-dihydroxybiphenyl 1,2-dioxygenase (bphc), were identified crystallographically as two histidines and a glutamic acid [sugiyama, k., et al. (1995) proc. jpn. acad., ser. b 71, 32-35; han, et al. ... | 1997 | 9047314 |
| new metabolites in the degradation of fluorene by arthrobacter sp. strain f101. | identification of new metabolites and demonstration of key enzyme activities support and extend the pathways previously reported for fluorene metabolism by arthrobacter sp. strain f101. washed-cell suspensions of strain f101 with fluorene accumulated 9-fluorenone, 4-hydroxy-9-fluorenone, 3-hydroxy-1-indanone, 1-indanone, 2-indanone, 3-(2-hydroxyphenyl) propionate, and a compound tentatively identified as a formyl indanone. incubations with 2-indanone produced 3-isochromanone. the growth yield wi ... | 1997 | 9055403 |
| arthrobacter ureafaciens sialidase isoenzymes, l, m1 and m2, cleave fucosyl gm1. | among bacterial, fungal and viral sialidases, the sialidase from arthrobacter ureafaciens has the unique property of cleaving sialic acids linked to the internal galactose of gangliotetraose. in this study, we examined the ability to cleave the internal sialic acids of gm1 and fucosyl gm1 of sialidases from several bacterial and fungal origins, including clostridium perfringens and vibrio cholerae. we found that a. ureafaciens sialidase could liberate the sialic acid of gm1 at the highest rate, ... | 1997 | 9076515 |
| a classification of dextran-hydrolysing enzymes based on amino-acid-sequence similarities. | 1997 | 9169623 | |
| cloning, overexpression, and mutagenesis of the gene for homoprotocatechuate 2,3-dioxygenase from brevibacterium fuscum. | homoprotocatechuate (hpca, 3,4-dihydroxyphenylacetate) is a central intermediate for the bacterial degradation of aromatic compounds. homoprotocatechuate 2,3-dioxygenase (hpcd) catalyzes the key ring cleavage step in the metabolism of hpca by the gram (+) bacterium brevibacterium fuscum to yield alpha-hydroxy-delta-carboxymethyl cis-muconic semialdehyde. a genomic dna library of b. fuscum was constructed in escherichia coli using a cosmid vector and screened by spraying the cells with hpca. one ... | 1997 | 9179284 |
| diversity and association of psychrophilic bacteria in antarctic sea ice. | the bacterial populations associated with sea ice sampled from antarctic coastal areas were investigated by use of a phenotypic approach and a phylogenetic approach based on genes encoding 16s rrna (16s rdna). the diversity of bacteria associated with sea ice was also compared with the bacterial diversity of seawater underlying sea ice. psychrophilic (optimal growth temperature, < or = 15 degrees c; no growth occurring at 20 degrees c) bacterial diversity was found to be significantly enriched i ... | 1997 | 9251193 |
| transformation of arabidopsis thaliana with the coda gene for choline oxidase; accumulation of glycinebetaine and enhanced tolerance to salt and cold stress. | glycinebetaine is one of the compatible solutes that accumulate in the chloroplasts of contain halotolerant plants when these plants are exposed to salt or cold stress. the coda gene for choline oxidase, the enzyme that converts choline into glycinebetaine, has previously been cloned from a soil bacterium, arthrobacter globiformis. transformation of arabidopsis thaliana with the cloned coda gene under the control of the 35s promoter of cauliflower mosaic virus enabled the plant to accumulate gly ... | 1997 | 9263456 |
| heavy metal resistant arthrobacter sp.--a tool for studying conjugational plasmid transfer between gram-negative and gram-positive bacteria. | the role of two heavy metal-resistant strains of the gram-positive genus arthrobacter sp. as a tool in studying conjugational plasmid transfer between gram-positive and gram-negative bacteria is described. the high nickel resistance and the cobalt resistance of arthrobacter sp. strain rm1/6 could be transferred to arthrobacter sp. strain ws14. incq plasmids (pkt240, pkt240::czc, pml10) could be mobilized from e. coli into arthrobacter spp. strains; antibiotic (km, ap, tc) and heavy metal (co) re ... | 1997 | 9265744 |
| isolation and characterization of highly (r)-specific n-acetyl-1-phenylethylamine amidohydrolase, a new enzyme from arthrobacter aurescens acr5b. | a new amidohydrolase deacetylating several n-acetyl-1-phenylethylamine derivatives (r)-specifically was found in arthrobacter aurescens acr5b. the strain was isolated from a wet haystack by enrichment culture with (r)-n-acetyl-1-phenylethylamine as the sole carbon source. (r) and (s)-n-acetyl-1-phenylethylamine do not serve as inducers for acylase formation. by improving the growth conditions the enzyme production was increased 47-fold. the amidohydrolase was purified to homogeneity leading to a ... | 1997 | 9237388 |
| molecular characterization of the bet genes encoding glycine betaine synthesis in sinorhizobium meliloti 102f34. | as a first step towards the elucidation of the molecular mechanisms responsible for the utilization of choline and glycine betaine (betaine) either as carbon and nitrogen sources or as osmoprotectants in sinorhizobium meliloti, we selected a tn5 mutant, lts23-1020, which failed to grow on choline but grew on betaine. the mutant was deficient in choline dehydrogenase (cdh) activity, failed to oxidize [methyl-14c]choline to [methyl-14c]betaine, and did not use choline, but still used betaine, as a ... | 1997 | 9141699 |
| classification of catechol 1,2-dioxygenase family: sequence analysis of a gene for the catechol 1,2-dioxygenase showing high specificity for methylcatechols from gram+ aniline-assimilating rhodococcus erythropolis an-13. | gram+ aniline-assimilating rhodococcus erythropolis an-13 (an-13) produces catechol 1,2-dioxygenase (c12o) showing high enzymatic activities for 3- and 4-methylcatechols [aoki et al. (1984) agric. biol. chem. 48, 2087-2095]. a 3.0 kb sau3ai fragment carrying a gene encoding c12o(cata) was cloned by selection of transformants showing c12o activity from a gene library of an-13. furthermore, we specified a 1.6 kb sali fragment containing cata from the sau3ai fragment by subcloning. sequence analysi ... | 1997 | 9034312 |
| species-specific beta-n-acetylgalactosaminylation of serum igg proteins. | lectin blot analysis of bovine, goat, human, rabbit and mouse serum immunoglobulin g (igg) samples revealed that wisteria floribunda agglutinin (wfa) binds to the heavy chains of bovine, goat and human serum igg proteins but not those of the rabbit and mouse proteins. wfa-positive light chain bands were also detected in bovine, goat and human serum igg samples only after the filters were treated with arthrobacter ureafaciens sialidase. the wfa-binding to these igg proteins was abolished by treat ... | 1997 | 9101715 |
| a novel arthrobacter beta-galactosidase with homology to eucaryotic beta-galactosidases. | an arthrobacter beta-galactosidase has homology with the lysosomal acid beta-galactosidases from humans and mice and with a xanthomonas manihotis enzyme. phylogenetic analysis of the deduced amino acid sequence showed an unusual pattern, with this procaryotic enzyme clustering within the animal clade. the gene encodes a subunit of 52 kda, and the enzyme appears to be active as a dimer. the enzyme hydrolyzed substrates with either a beta-1,4 or a beta-1,3 linkage. | 1997 | 9139931 |
| molecular cloning and heterologous expression of the isopullulanase gene from aspergillus niger a.t.c.c. 9642. | isopullulanase (ipu) from aspergillus niger a.t.c.c. (american type culture collection) 9642 hydrolyses pullulan to isopanose. ipu is important for the production of isopanose and is used in the structural analysis of oligosaccharides with alpha-1,4 and alpha-1,6 glucosidic linkages. we have isolated the ipua gene encoding ipu from the filamentous fungi a. niger a.t.c.c. 9642. the ipua gene encodes an open reading frame of 1695 bp (564 amino acids). ipu contained a signal sequence of 19 amino ac ... | 1997 | 9169610 |
| is1473, a putative insertion sequence identified in the plasmid pao1 from arthrobacter nicotinovorans: isolation, characterization, and distribution among arthrobacter species. | a putative insertion sequence (is1473) has been cloned and sequenced. the 1087-bp element was found between the moaa and the ndha genes in the upstream region of the nicotine dehydrogenase (ndh) operon in the 160-kb pao1 plasmid of arthrobacter nicotinovorans. it is flanked by an imperfect repeat of 33 bp and carries two overlapping open reading frames which, by programmed -1 translational frameshifting, may produce a transposase of 36.735 da with a pi = 10. 18. the deduced protein is similar to ... | 1997 | 9073580 |
| catalysis by a new sialidase, deaminoneuraminic acid residue-cleaving enzyme (kdnase sm), initially forms a less stable alpha-anomer of 3-deoxy-d-glycero-d-galacto-nonulosonic acid and is strongly inhibited by the transition state analogue, 2-deoxy-2, 3-didehydro-d-glycero-d-galacto-2-nonulopyranosonic acid, but not by 2-deoxy-2,3-didehydro-n-acetylneuraminic acid. | deaminoneuraminic acid residue-cleaving enzyme (kdnase sm) is a new sialidase that has been induced and purified from sphingobacterium multivorum. catalysis by this new sialidase has been studied by enzyme kinetics and 1h nmr spectroscopy. vmax/km values determined for synthetic and natural substrates of kdnase sm reveal that 4-methylumbelliferyl-kdn (kdnalpha2meumb, vmax/km = 0.033 min-1) is the best substrate for this sialidase, presumably because of its good leaving group properties. the tran ... | 1997 | 9038146 |
| identification of yeasts and coryneform bacteria from the surface microflora of brick cheeses. | coryneform bacteria and yeasts of 21 brick cheeses from six german dairies, produced by using undefined ripening cultures, were identified. arthrobacter nicotianae, brevibacterium linens, corynebacterium ammoniagenes, corynebacterium variabilis and rhodococcus fascians were found in significant numbers. out of 148 coryneform isolates 36 could not be identified at the species level. with the exception of a large rennet cheese, the coryneform microflora of rennet and acid cured cheeses were simila ... | 1997 | 9039559 |
| distribution of amine oxidases and amine dehydrogenases in bacteria grown on primary amines and characterization of the amine oxidase from klebsiella oxytoca. | the bacteria klebsiella oxytoca lmd 72.65 (atcc 8724), arthrobacter p1 lmd 81.60 (ncib 11625), paracoccus versutus lmd 80.62 (atcc 25364), escherichia coli w lmd 50.28 (atcc 9637), e. coli k12 lmd 93.68, pseudomonas aeruginosa pao1 lmd 89.1 (atcc 17933) and pseudomonas putida lmd 68.20 (atcc 12633) utilized primary amines as a carbon and energy source, although the range of amines accepted varied from organism to organism. the gram-negative bacteria k. oxytoca and e. coli as well as the gram-pos ... | 1997 | 9043125 |
| expression of sulfated gangliosides in the central nervous system. | several sulfated lipids were detected in the ganglioside fraction isolated from a cell line of oligodendrocyte progenitors that had been metabolically labeled with [35s] sulfate. separation of the ganglioside fraction by two-dimensional tlc showed that, except for galactosylceramide-sulfate, none of the sulfate-labeled lipids comigrated with those glycosphingolipids visualized by orcinol staining, indicating that these sulfolipids were quantitatively minor components. at least eight sulfate-labe ... | 1997 | 9003081 |
| pcp degradation is mediated by closely related strains of the genus sphingomonas. | there have been numerous reports in the literature of diverse bacteria capable of degrading pentachlorophenol (pcp). in order to gain further insight into the phylogenetic relationships of pcp-degrading bacteria, we examined four strains: arthrobacter sp. strain atcc 33790, flavobacterium sp. strain atcc 39723, pseudomonas sp. strain sr3, and sphingomonas sp. strain ra2. these organisms were isolated from different geographical locations and all of them degrade high concentrations (100-200 mg/l) ... | 1997 | 9004518 |
| cloning, sequencing, and expression of arthrobacter protophormiae endo-beta-n-acetylglucosaminidase in escherichia coli. | the gene encoding endo-beta-n-acetylglucosaminidase from arthrobacter protophormiae (endo-a) was cloned, and its nucleotide sequence was determined. a single open reading frame consisting of 1935 base pairs and encoding a polypeptide composed of signal peptides of 24 amino acids and a mature protein of 621 amino acids was found. the primary structure of endo-a exhibited significant homology with fo1f.10 gene product from caenorhabditis elegans and weak homology with peptide-n4-(n-acetyl-beta-d-g ... | 1997 | 9015383 |
| characterization of catechol catabolic genes from rhodococcus erythropolis 1cp. | the biochemical characterization of the muconate and the chloromuconate cycloisomerases of the chlorophenol-utilizing rhodococcus erythropolis strain 1cp previously indicated that efficient chloromuconate conversion among the gram-positive bacteria might have evolved independently of that among gram-negative bacteria. based on sequences of the n terminus and of tryptic peptides of the muconate cycloisomerase, a fragment of the corresponding gene has now been amplified and used as a probe for the ... | 1997 | 8990288 |
| clinical microbiology of coryneform bacteria. | coryneform bacteria are aerobically growing, asporogenous, non-partially-acid-fast, gram-positive rods of irregular morphology. within the last few years, there has been a massive increase in the number of publications related to all aspects of their clinical microbiology. clinical microbiologists are often confronted with making identifications within this heterogeneous group as well as with considerations of the clinical significance of such isolates. this review provides comprehensive informa ... | 1997 | 8993861 |
| a strain of arthrobacter that tolerates high concentrations of nitrate. | a gram-positive strain identified as arthrobacter globiformis cect 4500, tolerant to up to 1 m nitrate, was isolated from the grounds of a munitions factory. under strict aerobic conditions, this bacterium used a wide variety of c-sources to obtain the energy required for growth, which took place when the nitrate concentration in the medium was below 150 mm. cells of this bacterium growing in the absence of nitrate were seen as individual cells or forming pairs, whereas cells grown in the presen ... | 1997 | 15765585 |
| survival and phospholipid fatty acid profiles of surface and subsurface bacteria in natural sediment microcosms. | although starvation survival has been characterized for many bacteria, few subsurface bacteria have been tested, and few if any have been tested in natural subsurface porous media. we hypothesized that subsurface bacteria may be uniquely adapted for long-term survival in situ. we further hypothesized that subsurface conditions (sediment type and moisture content) would influence microbial survival. we compared starvation survival capabilities of surface and subsurface strains of pseudomonas fluo ... | 1997 | 16535578 |
| purification and characterization of two extracellular alkaline phosphatases from a psychrophilic arthrobacter isolate. | two extracellular, heat-labile alkaline phosphatases were purified from a psychrophilic arthrobacter isolate, d10. the enzymes were active over different ph ranges, used distinct substrates, and had different kinetic properties. each enzyme reacted specifically to its own antibody during immunoblot analysis. one had both monophosphatase and diesterase activities. | 1997 | 16535659 |
| biological potential of extraterrestrial materials. 2. microbial and plant responses to nutrients in the murchison carbonaceous meteorite. | meteoritic materials are investigated as potential early planetary nutrients. aqueous extracts of the murchison c2 carbonaceous meteorite are utilized as a sole carbon source by microorganisms, as demonstrated by the genetically modified pseudomonas fluorescence equipped with the lux gene. nutrient effects are observed also with the soil microorganisms nocardia asteroides and arthrobacter pascens that reach populations up to 5 x 10(7) cfu/ml in meteorite extracts, similar to populations in terre ... | 1997 | 11541241 |
| high affinity iron-uptake systems in vibrio damsela: role in the acquisition of iron from transferrin. | in this work, the high affinity iron-acquisition systems displayed by virulent and avirulent strains of vibrio damsela have been investigated. this species is an autochthonous member of marine ecosystems that can behave as an opportunistic pathogen for fish and mammals. all strains tested (i) were able to grow under the restricted conditions imposed by the iron chelators transferrin (tf) and eddha, (ii) secreted siderophores of hydroxamic type, other than aerobactin and desferal, that were able ... | 1997 | 12452589 |
| biodegradation of the mixture of 2,4,6-trichlorophenol, 4-chlorophenol, and phenol by a defined mixed culture. | two new strains, pseudomonas sp. tcp114 degrading 2,4,6-trichlorophenol (tcp) and arthrobacter sp. cpr706 degrading 4-chlorophenol (4-cp), were isolated through a selective enrichment procedure. both strains could also degrade phenol. the degradability of one component by a pure culture was strongly affected by the presence of other compounds in the medium. for example, when all three components (tcp, 4-cp, and phenol) were present in the medium, a pure culture of cpr706 could not degrade any of ... | 1997 | 12501340 |
| [microbial succession on lignite along with weathering]. | six different weathered lignite samples were examined by scanning electron microscopy. few microorganisms were observed on lignit just excavated and only spores and short hyphae were observed on lignite samples excavated 5 months, 1 year and 4 years before sampling. when lignite samples were moistened with distilled water and incubated for 10 days, actinomycetes proliferated significantly on lignite samples that were just excavated or excavated 5 months before sampling. the growth of bacteria wa ... | 1998 | 12548918 |
| bacterial composition of the biofilm on the surface of course sediment of the danube: with special reference to the clinically important bacteria. | on monthly intervals and over a period of 14 months (november, 1993-december, 1994) biofilm samples from sediments taken at the szentendre island on the danube were culturel and the isolated organisms were examined macromorphologically and micromorphogically and tested for oxidase and catalase production and their ability to oxidise and ferment glucose. the majority (85%) of the strains isolated were catalase positive. 43% were oxidase positive, 38% were glucose oxidisers and only 19% fermented ... | 1998 | 14666747 |
| cyanobacteria as agents for the control of pollution by pesticides and chlorinated organic compounds. | cyanobacteria are phototrophic aquatic micro-organisms that are found in a variety of environments, including polluted ones. fifteen strains of cyanobacteria that belong to three taxonomic groups are able to degrade lindane (λ-hexachlorocyclohexane, a recalcitrant pesticide). the initial degradation pathway has been studied in two filamentous nitrogen-fixing strains of anabaena sp. pcc 7120 and nostoc ellipsosporum. these cyanobacteria dechlorinated loindane first to pentachlorocyclohexene and t ... | 1998 | 21182708 |
| bioremediation of phenol by alkaliphilic bacteria isolated from alkaline lake of lonar, india. | phenol is an industrially important compound which has a wide range of applications. being highly soluble in water, it appears as the major pollutant in waste waters arising from both phenol manufacturing and from industrial units that utilise phenol. because of its toxicity, bioremediation of phenol is necessary. since some of the phenol-bearing industrial waste waters are alkaline in nature, use of alkaliphilic bacteria for bioremediation of phenol was investigated. alkaliphilic bacteria were ... | 1998 | 21182701 |
| crystallization of arthrobacter sp. strain 1c n-(1-d-carboxyethyl)-l- norvaline dehydrogenase and its complex with nad+. | the novel nad+-linked opine dehydrogenase from a soil isolate arthrobacter sp. strain 1c belongs to an enzyme superfamily whose members exhibit quite diverse substrate specificities. crystals of this opine dehydrogenase, obtained in the presence or absence of co-factor and substrates, have been shown to diffract to beyond 1.8 a resolution. x-ray precession photographs have established that the crystals belong to space group p21212, with cell parameters a = 104.9, b = 80.0, c = 45.5 a and a singl ... | 1998 | 9867431 |
| molecular evolution of hydantoinases. | the complete amino acid sequence of the hydantoinase from arthrobacter aurescens dsm 3745 has been derived by automated edman degradation. this is the first ever reported amino acid sequence of a non-atp-dependent hydantoinase, which hydrolyzes 5'-monosubstituted hydantoin derivatives l-selectively. a homology search performed in protein and nucleic acid databases retrieved only distantly related proteins. all of these are members of the recently described protein superfamily of amidohydrolases ... | 1998 | 9687026 |
| cloning and sequence determination of a phi aau2 gene whose product aborts the phage lytic cycle. | this communication describes the cloning of a 1.8-kb fragment from the genome of the corynephage phi aau2, which aborts the phage lytic cycle when cloned on a high-copy shuttle vector. the associated phenotype, called apld (aborting phage lytic development), was revealed by noting the reduced plaque size and lower efficiencies of plaquing of phi aau2 cp, a virulent derivative of phi aau2, on "arthrobacter aureus"-c70 apld+ cells. adsorption and phage dna transfection experiments showed evidence ... | 1998 | 9766220 |
| the acute phase response in the sudan plated lizard, gerrhosaurus major. | the sudan plated lizard (gerrhosaurus major), previously reported to be an afebrile species, was utilized in a series of experiments to test for various aspects of the acute phase response. treatment of individuals with the antibiotic baytril resulted in a slight (0.5 degree c) but significant reduction in mean selected body temperature (msbt), while treatment with saline did not lower msbt. nonantibiotic treatment individuals had depressed plasma iron levels (86.6 +/- 22.4 micrograms fe 100 ml- ... | 1998 | 9773512 |
| the 1.8 a crystal structure of the ycac gene product from escherichia coli reveals an octameric hydrolase of unknown specificity. | the ycac gene comprises a 621 base pair open reading frame in escherichia coli. the ycac gene product (ycacgp) is uncharacterized and has no assigned function. the closest sequence homologs with an assigned function belong to a family of bacterial hydrolases that catalyze isochorismatase-like reactions, but these have only low sequence similarity to ycacgp (approximately 20% amino acid identity). the ycacgp was obtained and identified during crystallization trials of an unrelated e. coli protein ... | 1998 | 9782055 |
| whipple's syndrome (uveitis, b27-negative spondylarthropathy, meningitis, and lymphadenopathy) associated with arthrobacter sp. infection. | to report an unusual case of whipple's disease, including uveitis, seronegative spondylarthropathy, meningitis, and lymphadenopathy, associated with an arthrobacter sp. infection. | 1998 | 9787360 |
| pbla8, from brevibacterium linens, belongs to a gram-positive subfamily of cole2-related plasmids. | a 3.1 kb dna fragment from pbla8, a brevibacterium linens cryptic plasmid, containing all the information required for autonomous replication was cloned and sequenced. using deletion analysis, the fragment essential and sufficient for autonomous replication was delimited to 1.5 kb. this fragment is characterized by the presence of an ori site located upstream of an operon encoding two proteins, repa and repb, both essential for replication. based on structural similarities and a strong conservat ... | 1998 | 9802024 |
| 3-ketosteroid-delta1-dehydrogenase of rhodococcus rhodochrous: sequencing of the genomic dna and hyperexpression, purification, and characterization of the recombinant enzyme. | the gene encoding 3-ketosteroid-delta1-dehydrogenase from rhodococcus rhodochrous was cloned and sequenced. the gene (ksdd) consists of 1,536 nucleotides and encodes an enzyme protein of 511 amino acid residues. the amino terminal methionine residue was deleted in the mature protein. the amino acids involved in the flavin binding site are conserved in the dehydrogenase sequence. the deduced amino acid sequence is highly homologous to that from arthrobacter simplex but less so to that from pseudo ... | 1998 | 9792929 |
| repeated application of carvone-induced bacteria to enhance biodegradation of polychlorinated biphenyls in soil. | carvone, the principal component of spearmint oil, induces biodegradation of polychlorinated biphenyls (pcb) by arthrobacter sp. strain b1b. this study investigated the effectiveness of the repeated application of carvone-induced bacteria for bioremediation of aroclor-1242-contaminated soil. control treatments compared a single inoculation of carvone-induced cells, repeated applications of noninduced cells, and repeated applications of cell-free carvone/fructose medium. the results showed that r ... | 1998 | 9830100 |
| enhancement of the tolerance of arabidopsis to high temperatures by genetic engineering of the synthesis of glycinebetaine. | arabidopsis thaliana was transformed with the coda gene for choline oxidase from arthrobacter globiformis under control of the 35s rna promoter of cauliflower mosaic virus. as a result, high levels of glycinebetaine accumulated in the seeds of transformed plants. transformation with the coda gene significantly enhanced the tolerance to high temperatures during the imbibition and germination of seeds, as well as during growth of young seedlings. the extent of enhancement of the tolerance to high ... | 1998 | 9839462 |
| allergic alveolitis among agricultural workers in eastern poland: a study of twenty cases. | the aim of this study was to identify the specific agents which caused extrinsic allergic alveolitis (eaa) in the selected group of 20 agricultural workers from eastern poland. the microbiological analysis of the samples of plant materials or dusts reported by the patients as causing symptoms has been carried out, followed by allergological tests (inhalation challenge, agar-gel precipitation test, inhibition of leukocyte migration, skin test) with extrinsic microbial antigens. it was found that ... | 1998 | 9852490 |