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species-specific beta-n-acetylgalactosaminylation of serum igg proteins.lectin blot analysis of bovine, goat, human, rabbit and mouse serum immunoglobulin g (igg) samples revealed that wisteria floribunda agglutinin (wfa) binds to the heavy chains of bovine, goat and human serum igg proteins but not those of the rabbit and mouse proteins. wfa-positive light chain bands were also detected in bovine, goat and human serum igg samples only after the filters were treated with arthrobacter ureafaciens sialidase. the wfa-binding to these igg proteins was abolished by treat ...19979101715
a novel arthrobacter beta-galactosidase with homology to eucaryotic beta-galactosidases.an arthrobacter beta-galactosidase has homology with the lysosomal acid beta-galactosidases from humans and mice and with a xanthomonas manihotis enzyme. phylogenetic analysis of the deduced amino acid sequence showed an unusual pattern, with this procaryotic enzyme clustering within the animal clade. the gene encodes a subunit of 52 kda, and the enzyme appears to be active as a dimer. the enzyme hydrolyzed substrates with either a beta-1,4 or a beta-1,3 linkage.19979139931
molecular characterization of the bet genes encoding glycine betaine synthesis in sinorhizobium meliloti 102f34.as a first step towards the elucidation of the molecular mechanisms responsible for the utilization of choline and glycine betaine (betaine) either as carbon and nitrogen sources or as osmoprotectants in sinorhizobium meliloti, we selected a tn5 mutant, lts23-1020, which failed to grow on choline but grew on betaine. the mutant was deficient in choline dehydrogenase (cdh) activity, failed to oxidize [methyl-14c]choline to [methyl-14c]betaine, and did not use choline, but still used betaine, as a ...19979141699
plant compounds that induce polychlorinated biphenyl biodegradation by arthrobacter sp. strain b1b.plant compounds that induced arthrobacter sp. strain b1b to cometabolize polychlorinated biphenyls (pcbs) were identified by a screening assay based on the formation of a 4,4'-dichlorobiphenyl ring fission product. a chemical component of spearmint (mentha spicata), l-carvone, induced arthrobacter sp. strain b1b to cometabolize aroclor 1242, resulting in significant degradation of 26 peaks in the mixture, including selected tetra- and pentachlorobiphenyls. evidence for pcb biodegradation include ...19979143124
the species composition of oligotrophic bacterial communities of lake balaton--a numerical analysis.from among the 565 aerobic and facultatively anaerobic, oligotrophic bacterial isolated obtained from the open water region of the balatonfüred-bay, 58 representative strains were selected for detailed taxonomic-physiological studies and numerical analyses as well as simultaneous comparisons with authentic bacterial strains isolated earlier by langó (1987) from different water habitat of the lake balaton. on the basis of 186 ceded differential diagnostic characteristics involved in these analyse ...19969147724
molecular cloning and heterologous expression of the isopullulanase gene from aspergillus niger a.t.c.c. 9642.isopullulanase (ipu) from aspergillus niger a.t.c.c. (american type culture collection) 9642 hydrolyses pullulan to isopanose. ipu is important for the production of isopanose and is used in the structural analysis of oligosaccharides with alpha-1,4 and alpha-1,6 glucosidic linkages. we have isolated the ipua gene encoding ipu from the filamentous fungi a. niger a.t.c.c. 9642. the ipua gene encodes an open reading frame of 1695 bp (564 amino acids). ipu contained a signal sequence of 19 amino ac ...19979169610
a classification of dextran-hydrolysing enzymes based on amino-acid-sequence similarities. 19979169623
cloning, overexpression, and mutagenesis of the gene for homoprotocatechuate 2,3-dioxygenase from brevibacterium fuscum.homoprotocatechuate (hpca, 3,4-dihydroxyphenylacetate) is a central intermediate for the bacterial degradation of aromatic compounds. homoprotocatechuate 2,3-dioxygenase (hpcd) catalyzes the key ring cleavage step in the metabolism of hpca by the gram (+) bacterium brevibacterium fuscum to yield alpha-hydroxy-delta-carboxymethyl cis-muconic semialdehyde. a genomic dna library of b. fuscum was constructed in escherichia coli using a cosmid vector and screened by spraying the cells with hpca. one ...19979179284
role of electrostatics at the catalytic metal binding site in xylose isomerase action: ca(2+)-inhibition and metal competence in the double mutant d254e/d256e.the catalytic metal binding site of xylose isomerase from arthrobacter b3728 was modified by protein engineering to diminish the inhibitory effect of ca2+ and to study the competence of metals on catalysis. to exclude ca2+ from site 2 a double mutant d254e/d256e was designed with reduced space available for binding. in order to elucidate structural consequences of the mutation the binary complex of the mutant with mg2+ as well as ternary complexes with bivalent metal ions and the open-chain inhi ...19979188736
[microflora of the air in sawmills as a potential occupational hazard: concentration and composition of microflora and immunologic reactivity of workers to microbial aeroallergens].microbiologial studies of the air and allergological examinations of the workers were performed in two sawmills processing deciduous wood (mainly oak) and in one sawmill processing coniferous wood (mainly pine). the concentration of microorganisms in the air was of the order 10(3)-10(4) cfu/m3. the most common organisms were corynebacteria (arthrobacter, corynebacterium, brevibacterium, microbacterium), spore-forming bacilli (bacillus), gram-negative bacteria (rahnella) and filamentous fungi (as ...19969190234
[microflora of the in furniture factors as a potential occupational hazard: concentration and composition of microflora and immunologic reactivity of workers to microbial aeroallergens].microbiologial studies of the air were performed in two furniture factories. the concentration of microorganisms in the air was low, being of the order 10(3) cfu/m3. the most common organisms were corynebacteria (arthrobacter, corynebacterium, brevibacterium, microbacterium) and fungi (aspergillus fumigatus, rhodotorula rubra). some of the species found in this environment possess known allergenic properties. allergological examinations of the workers with environmental aeroallergens have been p ...19969190236
16s rrna gene sequence of rubrobacter radiotolerans and its phylogenetic alignment with members of the genus arthrobacter, gram-positive bacteria, and members of the family deinococcaceae.the nearly complete sequence of the 16s rrna gene of an extremely highly radiotolerant bacterium, rubrobacter radiotolerans (reclassified from arthrobacter radiotolerans based on chemical characteristics), was determined by pcr amplification of the genomic dna followed by cloning of the amplified gene and sequencing by the dideoxynucleotide method. the sequence was aligned with the sequences of members of the genus arthrobacter and also with the sequences of representatives of the gram-positive ...19979226900
isolation and characterization of highly (r)-specific n-acetyl-1-phenylethylamine amidohydrolase, a new enzyme from arthrobacter aurescens acr5b.a new amidohydrolase deacetylating several n-acetyl-1-phenylethylamine derivatives (r)-specifically was found in arthrobacter aurescens acr5b. the strain was isolated from a wet haystack by enrichment culture with (r)-n-acetyl-1-phenylethylamine as the sole carbon source. (r) and (s)-n-acetyl-1-phenylethylamine do not serve as inducers for acylase formation. by improving the growth conditions the enzyme production was increased 47-fold. the amidohydrolase was purified to homogeneity leading to a ...19979237388
lectins binding on human sperm surface increase membrane permeability and stimulate acrosomal exocytosis.cross-linked complexes formed between certain lectins and their specific multivalent carbohydrates and glycoconjugates on the sperm surface were studied for their ability to modify sperm membrane permeability and to induce the acrosome reaction. wheat germ agglutinin (wga), concanavalin a (con a) and peanut agglutinin (pna) increased the proportions of human spermatozoa permeable to the impermeable propidium iodide (31.9 compared with 13.8%, 38.4 compared with 18.4% and 72.7 compared with 18.9% ...19969239678
leukosialin (cd43) is proteolytically cleaved from stimulated hmc-1 cells.leukosialin (cd43), the major sialoprotein on circulating leukocytes, has been previously described to be down-regulated on neutrophils following activation with phorbol myristate acetate (pma). the other single cells previously examined, blood lymphocytes, do not down-regulate cd43 when stimulated by pma. recently, we have characterized leukosialin on the human mast cell line hmc-1 and observed that leukosialin is down-regulated after stimulation with pma. in the present study, we have investig ...19979241533
genetic identification of members of the genus corynebacterium at genus and species levels with 16s rdna-targeted probes.16s rrna gene-targeted probes were designed for the identification of corynebacteria at the genus and species levels. the genus-specific probe hybridized all clinically important members of the genus corynebacterium and could distinguish them from other coryneform bacteria and phylogenetically related high g+c% gram-positive bacteria, including actinomyces, rhodococcus, gordona, nocardia, streptomyces, brevibacterium and mycobacterium. the species-specific probes for c. jeikeium and c. diphtheri ...19979251056
diversity and association of psychrophilic bacteria in antarctic sea ice.the bacterial populations associated with sea ice sampled from antarctic coastal areas were investigated by use of a phenotypic approach and a phylogenetic approach based on genes encoding 16s rrna (16s rdna). the diversity of bacteria associated with sea ice was also compared with the bacterial diversity of seawater underlying sea ice. psychrophilic (optimal growth temperature, < or = 15 degrees c; no growth occurring at 20 degrees c) bacterial diversity was found to be significantly enriched i ...19979251193
transformation of arabidopsis thaliana with the coda gene for choline oxidase; accumulation of glycinebetaine and enhanced tolerance to salt and cold stress.glycinebetaine is one of the compatible solutes that accumulate in the chloroplasts of contain halotolerant plants when these plants are exposed to salt or cold stress. the coda gene for choline oxidase, the enzyme that converts choline into glycinebetaine, has previously been cloned from a soil bacterium, arthrobacter globiformis. transformation of arabidopsis thaliana with the cloned coda gene under the control of the 35s promoter of cauliflower mosaic virus enabled the plant to accumulate gly ...19979263456
heavy metal resistant arthrobacter sp.--a tool for studying conjugational plasmid transfer between gram-negative and gram-positive bacteria.the role of two heavy metal-resistant strains of the gram-positive genus arthrobacter sp. as a tool in studying conjugational plasmid transfer between gram-positive and gram-negative bacteria is described. the high nickel resistance and the cobalt resistance of arthrobacter sp. strain rm1/6 could be transferred to arthrobacter sp. strain ws14. incq plasmids (pkt240, pkt240::czc, pml10) could be mobilized from e. coli into arthrobacter spp. strains; antibiotic (km, ap, tc) and heavy metal (co) re ...19979265744
tissue-specific heterogeneity in alpha-dystroglycan sialoglycosylation. skeletal muscle alpha-dystroglycan is a latent receptor for vicia villosa agglutinin b4 masked by sialic acid modification.because the polypeptide core of alpha-dystroglycan is encoded by a single gene, the difference in apparent molecular mass between alpha-dystroglycans expressed in various tissues is presumably due to differential glycosylation. however, little is presently known about the tissue-specific differences in alpha-dystroglycan glycosylation and whether these modifications may confer functional variability to alpha-dystroglycan. we recently observed that laminin-1 binding to skeletal muscle alpha-dystr ...19979268382
capa, a cspa-like gene that encodes a cold acclimation protein in the psychrotrophic bacterium arthrobacter globiformis si55.by use of arthrobacter globiformis si55, a psychrotrophic bacterium capable of growth between -5 and +32 degrees c, we cloned and sequenced capa, a gene homologous to cspa encoding the major cold shock protein in escherichia coli. the deduced protein sequence has a high level of identity with the sequences of other cspa-related proteins from various sources, and no particular residue or domain that could be specific to cold-adapted microorganisms emerged. we show that capa was produced very rapi ...19979294420
topaquinone-dependent amine oxidases: identification of reaction intermediates by raman spectroscopy.resonance raman (rr) spectroscopy has proven to be an excellent technique for providing structural information about the 2,4, 5-trihydroxyphenylalaninequinone (tpq) cofactor and for identifying the source of oxygen atoms during the posttranslational synthesis of the cofactor. through specific labeling of the c2, c4, and c5 oxygens of tpq in phenylethylamine oxidase (peao) from arthrobacter globiformis, we have identified the c=o stretch of the c5 carbonyl at 1683 cm-1 (-27 in 18o) and the c=o st ...19979298968
phylogenetic characterization of bacteria in the subsurface microbial culture collection.the subsurface microbial culture collection (smcc) was established by the u.s. dept. of energy (doe) and contains nearly 10,000 strains of microorganisms (mostly bacteria) isolated from terrestrial subsurface environments. selected groups of bacterial isolates from three sample sites situated above geochemically and hydrologically different subsurface environments have been characterized by phylogenetic analysis of 16s ribosomal rna (rrna) gene nucleotide sequences. among these isolates were mem ...19979299704
detection of heavy metal ion resistance genes in gram-positive and gram-negative bacteria isolated from a lead-contaminated site.resistance to a range of heavy metal ions was determined for lead-resistant and other bacteria which had been isolated from a battery-manufacturing site contaminated with high concentration of lead. several gram-positive (belonging to the genera arthrobacter and corynebacterium) and gram-negative (alcaligenes species) isolates were resistant to lead, mercury, cadmium, cobalt, zinc and copper, although the levels of resistance to the different metal ions were specific for each isolate. polymerase ...19979342884
isolation and characterization of d-threonine aldolase, a pyridoxal-5'-phosphate-dependent enzyme from arthrobacter sp. dk-38.d-threonine aldolase is an enzyme that catalyzes the cleavage of d-threonine into glycine and acetaldehyde. its activity was found in several genera of bacteria such as arthrobacter, alcaligenes, xanthomonas, and pseudomonas, but not in yeasts or fungi. the enzyme was purified to homogeneity from one strain, arthrobacter sp. dk-38. the enzyme appeared to consist of a single polypeptide chain with an apparent molecular mass of 51 kda. this enzyme, as well as l-threonine aldolase, requires pyridox ...19979346293
purification and characterization of polyamine aminotransferase of arthrobacter sp. tmp-1.polyamine aminotransferase of arthrobacter sp. tmp-1 was induced by 1,3-diaminopropane (dap), n-3-aminopropyl-1,3-diaminopropane (norspermidine), spermidine, and spermine, but not by putrescine. the enzyme was purified to homogeneity. its molecular weight and subunit size were 129,000 and 64,000, respectively. its absorption spectrum had maxima at 280 and 420 nm and a shoulder at about 350 nm, and changes were observed upon the addition of dap, putrescine, and sodium borohydride. the spectrum an ...19979348081
action of polyamine aminotransferase on norspermidine.the norspermidine-pyruvate reaction catalyzed by polyamine aminotransferase from arthrobacter sp. tmp-1 formed n-3-aminopropyl-3-aminopropionaldehyde (apapal), l-alanine, 1,3-diaminopropane (dap), allylamine, and acrolein, and the relative rates of formation of the latter four products were 24, 3.3, 2.3, and 1.2%, respectively, of the rate of the dap-pyruvate transamination. the identification of apapal was done by 13c-nmr after it had been enzymatically oxidized to n-3-aminopropyl-beta-alanine ...19979348082
rapid physiological characterization of microorganisms by biosensor technique.eleven microorganisms, arxula adeninivorans ls3, candida boidinii dsm 70034, candida lactis-condensi dsm 70635, pichia jadinii dsm 2361, pichia minuta dsm 7018, kluyveromyces lactis dsm 4394, pseudomonas putida dsm 50026, alcaligenes sp. dsm 30002, arthrobacter nicotianae dsm 20123 as well as issatchenkia orientalis dsm 70077 and rhodococcus erythropolis dsm 311 were characterized by the sensor technique by injection of 30 different substrates and substrate mixtures. the obtained data which are ...19979352658
isolation of alu1-p gene encoding a protein with aluminum tolerance activity from arthrobacter viscosus.we isolated a dna fragment (alu1-p) encoding a protein with an activity of aluminum tolerance from an al tolerant soil microorganism, arthrobacter viscosus. this microorganism was isolated from acidic tea field soils. the cloned dna is composed of 1090 nucleotides, which has one open-reading frame without any stop codon. however, when the dna fragment was transferred into escherichia coli, a microorganism susceptible to al toxicity, it endowed e. coli with al tolerance. the deduced amino acid se ...19979367855
crystal structures of the copper-containing amine oxidase from arthrobacter globiformis in the holo and apo forms: implications for the biogenesis of topaquinone.the crystal structures of the copper enzyme phenylethylamine oxidase from the gram-positive bacterium arthrobacter globiformis (agao) have been determined and refined for three forms of the enzyme: the holoenzyme in its active form (at 2.2 a resolution), the holoenzyme in an inactive form (at 2.8 a resolution), and the apoenzyme (at 2.2 a resolution). the holoenzyme has a topaquinone (tpq) cofactor formed from the apoenzyme by the post-translational modification of a tyrosine residue in the pres ...19979405045
[dynamics of bacteria from the phyllosphere and leaves of soy (glycine max l. merrill) in field conditions].the surfaces of aerial plant provide a habitat for epiphitic microorganisms many of which are capable of influencing the growth of pathogens. in this study, fluctuation of bacterial population occurring in different growth stages of soybean leaves (glycine max l merrill) was examined using the dilution plate method. three culture media were applied; invariably, the numbers of bacteria increased with increasing plant age. the total number of bacteria living on the detached leaves were ten times h ...19979411487
biotreatment of ammonia from air by an immobilized arthrobacter oxydans ch8 biofilter.a heterotrophic arthrobacter oxydans ch8 that was capable of removing nh3 from nh3 containing gas was isolated from livestock farming wastewater. the a. oxydans ch8 was immobilized with calcium alginate packed into filter column. metered nh3-containing gas was partially humidified and passed through the glass column. extensive tests including the removal characteristics, the removal efficiencies, and the metabolic products of nh3 by a. oxydans ch8 were conducted. additionally, the operation crit ...19979413137
molybdate-uptake genes and molybdopterin-biosynthesis genes on a bacterial plasmid--characterization of moea as a filament-forming protein with adenosinetriphosphatase activity.a gene cluster consisting of homologs to escherichia coli moaa, moea, moac and moae, which encode enzymes involved in the biosynthesis of molybdopterin cofactor (moco), and to moda, modb and modc, which encode a high-affinity molybdate transporter, were identified on pao1 of arthrobacter nicotinovorans near genes of molybdopterin-dependent enzymes involved in nicotine degradation. this gene arrangement suggests a coordinated expression of the moco-dependent and the moco-biosynthesis genes and sh ...19979428706
molecular cloning of levan fructotransferase gene from arthrobacter nicotinovorans gs-9 and its expression in escherichia coli.the gene encoding an extracellular levan fructotransferase, designated the lft gene, was cloned from the genomic dna of arthrobacter nicotinovorans gs-9, and expressed in escherichia coli. it was found that a single open reading frame consisted of 1554 base pairs that encoded a polypeptide composed of a signal peptide of 33 amino acids and a mature protein of 484 amino acids (m(r) 53,152), and it was also found that a putative ribosome-binding site was present in the upstream from the orf. the p ...19979438987
a genetic and functional analysis of the unusually large variable region in the m.alui dna-(cytosine c5)-methyltransferase.the m.alui dna-(cytosine c5)-methyltransferase (5mc methylase) acts on the sequence 5'-agct-3'. the amino acid sequences of known 5mc methylases contain ten conserved motifs, with a variable region between motifs viii and ix that contains one or more "target-recognizing domains" (trds) responsible for dna sequence specificity. monospecific 5mc methylases are believed to have only one trd, while multispecific 5mc methylases have as many as five. m.alui has the second-largest variable region of al ...19979439564
characterization of the interaction between vp8 of bovine rotavirus c486 and cellular components on ma-104 cells and erythrocytes.rotavirus vp8*, the n-terminal trypsin cleavage product of vp4, has been shown to bind to ma-104 cells and human o type erythrocytes. to examine whether bacterially expressed vp8* binds to cellular components of ma-104 cells, the vp8* (aa 1-247) was expressed in e. coli and radiolabelled with 35s-methionine. the radiolabelled rvp8* was immunoprecipitated with antiserum to bovine rotavirus c486 (brv). the rvp8* was found to bind to ma-104 cells and its binding was competed by brv. to study the in ...19989442941
structural and immunochemical characterization of beta-1,2-linked mannobiosyl phosphate residue in the cell wall mannan of candida glabrata.a mannan of candida glabrata ifo 0622 digested by arthrobacter exo-alpha-mannosidase and a beta-1,2-linked mannobiose obtained from the parent mannan by acid treatment was analyzed using 13c nuclear magnetic resonance spectroscopy. the results show that the beta-1, 2-linked mannobiosyl residue is esterified to a phosphate group through position c-1 in the alpha-configuration, manbeta1- 2manalpha1-hpo3-. the results of immunochemical assays of these mannans using the commercial antigenic factor s ...19989477251
structural study of the o-linked sugar chains of human leukocyte tyrosine phosphatase cd45.the o-linked sugar chains of the human leukocyte cell surface glycoprotein cd45 were released as tritium-labeled oligosaccharides by beta-elimination in the presence of nab3h4. mono q column chromatography revealed that they comprise neutral (64%) and acidic (36%) oligosaccharides, the latter of which were converted to neutral ones by arthrobacter ureafaciens sialidase treatment. structural studies of each oligosaccharide fractionated on a bio-gel p-4 column by sequential exoglycosidase digestio ...19989492296
asking for the indicator function of bioassays evaluating soil contamination: are bioassay results reasonable surrogates of effects on soil microflora?in evaluating the biological effect of solid materials like soil a bacterial contact assay often shows higher sensitivity than elutriate testing. results of the bacillus cereus contact assay for some environmental important toxicants are presented in this article. a comparison with another heterotrophic soil bacterium, arthrobacter globiformis, shows comparable sensitivity. in a bioassay approach organisms at the level of individuals or populations are exposed to soil material to determine the s ...19989493327
biochemical synthesis of several chiral insecticide intermediates and mechanisms of action of relevant enzymes.efficient biochemical processes were developed for the synthesis of several chiral alcohol and acid intermediates of insecticides by a combination of strictly stereoselective hydrolytic enzyme-catalyzed reactions and subsequent chemical transformations with inversion or racemization of the chiral center of the undesired antipodes. the whole amounts of starting racemic mixtures are converted to desired stereoisomers in the processes, which are generally applicable to the industrial productions of ...19989501511
enzymatic synthesis of a neoglycoconjugate by transglycosylation with arthrobacter endo-beta-n-acetylglucosaminidase: a substrate for colorimetric detection of endo-beta-n-acetylglucosaminidase activity.the transglycosylation activity of endo-beta-n-acetylglucosaminidase from arthrobacter protophormiae was used for the enzymatic synthesis of a novel oligosaccharide, man6glcnac-p-nitrophenyl-alpha-d-glucose (man6glcnac-glc-pnp). the reaction was efficiently induced in aqueous solution containing dimethyl sulfoxide. in the medium containing 20% (v/v) dimethyl sulfoxide with 0.1 m glc-pnp as an acceptor, the transglycosylation attained yields of 75% by high-performance anion-exchange chromatograph ...19989514782
the conformational change induced by fad in covalently flavinylated 6-hydroxy-d-nicotine oxidase does not require (8alpha)fad-(n3)histidyl bond formation.the contribution of (8alpha)-(n3)histidyl bond formation to the conformation of covalently flavinylated proteins was investigated by trypsin treatment of wild type and mutant versions of a model enzyme, 6-hydroxy-d-nicotine oxidase (6-hdno) of arthrobacter nicotinovorans. in the absence of fad, apo-6-hdno exhibited a conformation exposing a protease accessible site. holoenzyme formation through fad-attachment to his71 induced a conformational change in the protein that shielded the trypsin recog ...19989538227
gene cluster for creatinine degradation in arthrobacter sp. te1826.the genes encoding creatininase (crna; 258 residues) and creatinase (crea; 411 residues) from arthrobacter sp. te1826 were cloned and sequenced. the genes form a cluster with the sarcosine oxidase gene (soxa) and its regulator gene (soxr), which were cloned previously. the deduced amino acid sequences of crna and crea show 35.9% and 63.1% identity, respectively to the corresponding pseudomonas enzymes. crna and crea were purified from the recombinant strains and characterized. other open reading ...19989563845
degradation of geosmin-like compounds by selected species of gram-positive bacteria.biodegradation of geosmin produced by streptomyces halstedii (a-1 strain) was investigated using seven gram-positive bacteria. bacterial action on the a-1 extract was followed at 15, 45 and 120 min. bacillus cereus ssp. thuringiensis hd-1, b. cereus 3711 and b. cereus ssp. mycoides 4379 showed no effect. however, arthrobacter atrocyaneus, arth. globiformis, chlorophenolicus n-1053 and rhodococcus maris caused a colour change from blue to yellow (positive reaction). these data suggest that geosmi ...19989569688
structural studies on a sulfated polysaccharide from an arthrobacter sp. by nmr spectroscopy and methylation analysis.structural characterization of a sulfated polysaccharide peptidoglycan complex (sp-pg) from an arthrobacter sp. was performed by nmr spectroscopy and methylation analysis. in order to simplify the analyses, the desulfated sp-pg was used. nmr spectroscopy revealed the presence of a trisaccharide repeating unit and a disaccharide repeating unit. the trisaccharide unit was composed of two galactofuranosides and one glucopyranoside, and the disaccharide unit was of two galactopyranosides, as shown b ...19979581278
demethylation of acridine orange by arthrobacter globiformis. 19989595195
transfer of plasmid pto1 from escherichia coli to various representatives of the order actinomycetales by intergeneric conjugation.plasmid pto1 containing the orit fragment from rk2, the escherichia coli replication function from pbr322, and a dna fragment of actinophage phi c31 with the attachment site was transferred from e. coli s17-1 to strains of the genera actinomadura, arthrobacter, micromonospora, nocardia, rhodococcus, and to 16 strains of the genus streptomyces. the frequency of conjugant formation was 1 x 10(-3)-1 x 10(-5) depending on the strain. hybridization experiments demonstrated that plasmid pto1 integrate ...19989595662
bacterial density and survey of cultivable heterotrophs in the surface water of a freshwater marsh habitat of anopheles quadrimaculatus larvae (diptera: culicidae).we examined surface water samples collected in september and october 1994 from a freshwater marsh habitat containing larval anopheles quadrimaculatus mosquitoes. bacterial densities in direct microscopic counts ranged from 9.7 x 10(5) to 1.3 x 10(7) cells/ml. densities of cultivable bacteria on trypticase soy agar medium ranged from 1.0 to 1.5 x 10(5) cells/ml. the majority of 888 isolates were gram-positive rods (41%) followed by gram-negative rods (28%). analysis of the cellular fatty acid pro ...19989599327
characteristics of arthrobacter cumminsii, the most frequently encountered arthrobacter species in human clinical specimens.during a 2-year period, 10 strains of arthrobacter cumminsii were isolated in or received by a swiss routine clinical bacteriology laboratory, and 5 further isolates were referred to a swedish bacteriology reference center over a 5-year period, making a. cumminsii the most frequently encountered arthrobacter species in these two laboratories. the present report outlines the clinical features of the 15 a. cumminsii strains and presents an extended biochemical characterization of this microorganis ...19989620373
biochemical characterization of a haloalcohol dehalogenase from arthrobacter erithii h10a.arthrobacter erithii h10a possesses two enzymes capable of catalyzing the dehalogenation of vicinal halohydrins which have been designated as dehalogenases deha and dehc. the deha dehalogenase demonstrated greater activity toward 1,3-dichloro-2-propanol (1,3-dcp) while the dehc dehalogenase showed higher activity toward 3-chloro-1,2-propanediol (3-cpd) and brominated alcohols. the deha dehalogenase was composed of two non-identical subunits (relative molecular mass of 31.5 and 34 kda) which prob ...19989621448
isolation of a beta-galactosidase-encoding gene from bacillus licheniformis: purification and characterization of the recombinant enzyme expressed in escherichia coli.the bacillus licheniformis beta-galactosidase gene, lacbl, was cloned on a 5.8-kb hindiii fragment into pbr322 and expressed by its own promoter in escherichia coli. deletion and complementation analysis showed that the enzyme-encoding region was located on a 4. 1-kb hindiii-clai fragment. the transcription region for the lacbl was identified on this fragment with promoter- and terminator-probe plasmids. the deduced sequence of 149 aa of the n-terminal part of lacbl showed aa sequence homology w ...19989625788
structural model of dex protein from penicillium minioluteum and its implications in the mechanism of catalysis.the dex gene encodes an extracellular dextranase (ec 3.2.1.11); this enzyme hydrolyzes the alpha(1,6) glucosidic bond contained in dextran to release small isomaltosaccharides. sequence analysis has revealed only one homologous sequence, cb-8 protein, from arthrobacter sp., with 30% sequence identity. the secondary structure prediction for dex was corroborated by circular dichroism measurements. to explore the possibility that dex protein might adopt a fold similar to any known structure, we con ...19989626695
metabolism of nitrate esters by a consortium of two bacteria.the products of condensation of organic alcohols and nitric acid are nitrate esters with the general structure c-o-no2. these products are widely employed as vasodilators and explosives, and are true xenobiotic compounds, as they do not occur in nature. we have isolated and characterized a consortium of two microorganisms, arthrobacter ilicis and agrobacterium radiobacter, that mineralized recalcitrant ethylene glycol dinitrate. the arthrobacter strain was the actual degrading microorganism, alt ...19969630893
molecular cloning of a light-inducible gene (lipa) encoding a novel pilin from arthrobacter photogonimos.development of pili on cells of arthrobacter photogonimos is induced by photo-oxidative conditions. the nucleotide sequence was determined of a light-inducible gene (lipa) that encodes the precursor of a light-inducible pilin (designated lip), a polypeptide of 212 amino acids. the n-terminal leader peptide includes a typical signal sequence with a consensus cleavage site for signal peptidase 1 after residue 28, which should generate n-terminal arginine. however, the next amino acid, alanine, is ...19989631550
a novel metal-activated pyridoxal enzyme with a unique primary structure, low specificity d-threonine aldolase from arthrobacter sp. strain dk-38. molecular cloning and cofactor characterization.the gene encoding low specificity d-threonine aldolase, catalyzing the interconversion of d-threonine/d-allo-threonine and glycine plus acetaldehyde, was cloned from the chromosomal dna of arthrobacter sp. strain dk-38. the gene contains an open reading frame consisting of 1,140 nucleotides corresponding to 379 amino acid residues. the enzyme was overproduced in recombinant escherichia coli cells and purified to homogeneity by ammonium sulfate fractionation and three-column chromatography steps. ...19989642221
effects of difructose anhydride iii on calcium absorption in small and large intestines of rats.difructose anhydride iii (dfa iii; di-d-fructo-furanose 1,2':2,3' dianhydride) was prepared from inulin with arthrobacter sp. h65-7 inulin fructotransferase (depolymerizing (inulase ii; ec 2.4.1.93). dfa iii is not hydrolyzed by enzymes in the small intestine, but is metabolized by microorganisms in the large intestine. we investigated the effects of dfa iii on calcium absorption in two experiments. in the in vivo experiment, we examined the effects of dfa iii, fructooligosaccharides, and raffin ...19989648212
substrate-dependent enantioselectivity of a novel hydantoinase from arthrobacter aurescens dsm 3745: purification and characterization as new member of cyclic amidases.a hydantoinase from arthrobacter aurescens dsm 3745 has been purified to homogeneity with a yield of 77% using a three-step purification procedure. the active enzyme is a tetramer consisting of four identical subunits, each with a molecular mass of 49670 da as determined by mass spectrometry. the n-terminal amino acid sequence of the enzyme indicates sequence identities to cyclic amidases involved in the nucleotide metabolism as the d-hydantoinase from agrobacterium radiobacter (53%), the d-sele ...19989650283
crystal structure and active site location of n-(1-d-carboxylethyl)-l-norvaline dehydrogenase.opine dehydrogenases catalyze the nad(p)h-dependent reversible reaction to form opines that contain two asymmetric centers exhibiting either (l,l) or (d,l) stereochemistry. the first structure of a (d,l) superfamily member, n-(1-d-carboxylethyl)-l-norvaline dehydrogenase (cendh) from arthrobacter sp. strain 1c, has been determined at 1.8 a resolution and the location of the bound nucleotide coenzyme has been identified. six conserved residues cluster in the cleft between the enzyme's two domains ...19989665174
purification and characterization of catechol 1,2-dioxygenase from rhodococcus rhodochrous ncimb 13259 and cloning and sequencing of its cata gene.a method was developed for the purification of catechol 1, 2-dioxygenase from rhodococcus rhodochrous ncimb 13259 that had been grown in the presence of benzyl alcohol. the enzyme has very similar apparent km (1-2 microm) and vmax (13-19 units/mg of protein) values for the intradiol cleavage of catechol, 3-methylcatechol and 4-methylcatechol and it is optimally active at ph9. cross-linking studies indicate that the enzyme is a homodimer. it contains 0.6 atoms of fe per subunit. the enzyme was cr ...19989677336
molecular evolution of hydantoinases.the complete amino acid sequence of the hydantoinase from arthrobacter aurescens dsm 3745 has been derived by automated edman degradation. this is the first ever reported amino acid sequence of a non-atp-dependent hydantoinase, which hydrolyzes 5'-monosubstituted hydantoin derivatives l-selectively. a homology search performed in protein and nucleic acid databases retrieved only distantly related proteins. all of these are members of the recently described protein superfamily of amidohydrolases ...19989687026
arthrobacter, brachybacterium and planococcus isolates identified from antarctic sea ice brine. description of planococcus mcmeekinii, sp. nov.three facultative psychrophilic gram-positive bacterial strains were isolated from brine samples from the sea ice community in antarctica. all strains were coccoid to rod-shaped and exhibited broad salinity and temperature ranges for growth. the three strains were subjected to 16s rdna sequencing and subsequent phylogenetic analysis. all possess unique 16s rdna sequences indicating they are new, previously unreported organisms. phylogenetic analyses coupled with phenotypic characterization indic ...19989704115
intraspecific variation of unusual phospholipids from corynebacterium spp. containing a novel fatty acid.the novel fatty acid trans-9-methyl-10-octadecenoic acid was isolated from the coryneform bacterial strain lmg 3820 (previously misidentified as arthrobacter globiformis) and identified by spectroscopic methods and chemical derivatization. this fatty acid is attached to the unusual lipid acyl phosphatidylglycerol. five different species of this lipid type were identified; their structures were elucidated by tandem mass spectrometry and are reported here for the first time. additionally, we ident ...19989721308
description of arthrobacter creatinolyticus sp. nov., isolated from human urine.three strains of creatinine-hydrolysing bacteria isolated from human urine were characterized taxonomically. they were aerobic, non-spore-forming, gram-positive rods with the peptidoglycan of the cell wall containing lysine. mk-8 and mk-9 were found to be the major types of menaquinone. the g + c content of the dna was 66-67 mol%. the 16s rrna sequence of one strain (gifu 12498) was determined and aligned with other high-g + c-content gram-positive rods from different genera. following phylogene ...19989731280
corynebacterium striatum chloramphenicol resistance transposon tn5564: genetic organization and transposition in corynebacterium glutamicum.the clinical isolate corynebacterium striatum m82b (formerly corynebacterium xerosis m82b) carries the 50-kb r-plasmid ptp10 conferring resistance to the antibiotics chloramphenicol, erythromycin, kanamycin, and tetracycline. dna sequence analysis of the chloramphenicol resistance region revealed the presence of the 4155-bp transposable element tn5564. the ends of tn5564 are identical 22-bp inverted repeats flanked by a 6-bp target site duplication. the central region of tn5564 encodes the chlor ...19989735314
exploring a channel to the active site of copper/topaquinone-containing phenylethylamine oxidase by chemical modification and site-specific mutagenesis.copper amine oxidase contains an organic redox cofactor, 2,4, 5-trihydroxyphenylalaninequinone (topaquinone, tpq), derived by the post-translational modification of a specific tyrosyl residue. to identify amino acid residues participating in the biogenesis of tpq in the recombinant phenylethylamine oxidase from arthrobacter globiformis, we have modified the copper/tpq-less apoenzyme and the copper/tpq-containing holoenzyme with 4-fluoro-7-nitrobenzo-2-oxa-1, 3-diazole (nbd-f). in the apoenzyme m ...19989760229
purification and characterization of four catechol 1,2-dioxygenase isozymes from the benzamide-assimilating bacterium arthrobacter species ba-5-17.when arthrobacter sp. ba-5-17 was grown on benzamide, the bacterium synthesized four different catechol 1,2-dioxygenase (cd, ec 1.13.11.1) isozymes (cd-i, ii, iii-1, and iii-2). we purified each cd to homogeneity by a series of column chromatography. the molecular masses of the four cds were between 68 and 72 kda. the enzymes were made up of two identical subunits each with the molecular mass of 33 kda. cd-i and ii were indistinguishable in enzymatic properties tested. most properties of cd-iii- ...19989760749
crystallization of arthrobacter sp. strain 1c n-(1-d-carboxyethyl)-l-norvaline dehydrogenase and its complex with nad+the novel nad+-linked opine dehydrogenase from a soil isolate arthrobacter sp. strain 1c belongs to an enzyme superfamily whose members exhibit quite diverse substrate specificites. crystals of this opine dehydrogenase, obtained in the presence or absence of co-factor and substrates, have been shown to diffract to beyond 1.8 å resolution. x-ray precession photographs have established that the crystals belong to space group p21212, with cell parameters a = 104.9, b = 80.0, c = 45.5 å and a single ...19989761832
cloning and sequence determination of a phi aau2 gene whose product aborts the phage lytic cycle.this communication describes the cloning of a 1.8-kb fragment from the genome of the corynephage phi aau2, which aborts the phage lytic cycle when cloned on a high-copy shuttle vector. the associated phenotype, called apld (aborting phage lytic development), was revealed by noting the reduced plaque size and lower efficiencies of plaquing of phi aau2 cp, a virulent derivative of phi aau2, on "arthrobacter aureus"-c70 apld+ cells. adsorption and phage dna transfection experiments showed evidence ...19989766220
the acute phase response in the sudan plated lizard, gerrhosaurus major.the sudan plated lizard (gerrhosaurus major), previously reported to be an afebrile species, was utilized in a series of experiments to test for various aspects of the acute phase response. treatment of individuals with the antibiotic baytril resulted in a slight (0.5 degree c) but significant reduction in mean selected body temperature (msbt), while treatment with saline did not lower msbt. nonantibiotic treatment individuals had depressed plasma iron levels (86.6 +/- 22.4 micrograms fe 100 ml- ...19989773512
the 1.8 a crystal structure of the ycac gene product from escherichia coli reveals an octameric hydrolase of unknown specificity.the ycac gene comprises a 621 base pair open reading frame in escherichia coli. the ycac gene product (ycacgp) is uncharacterized and has no assigned function. the closest sequence homologs with an assigned function belong to a family of bacterial hydrolases that catalyze isochorismatase-like reactions, but these have only low sequence similarity to ycacgp (approximately 20% amino acid identity). the ycacgp was obtained and identified during crystallization trials of an unrelated e. coli protein ...19989782055
whipple's syndrome (uveitis, b27-negative spondylarthropathy, meningitis, and lymphadenopathy) associated with arthrobacter sp. infection.to report an unusual case of whipple's disease, including uveitis, seronegative spondylarthropathy, meningitis, and lymphadenopathy, associated with an arthrobacter sp. infection.19989787360
3-ketosteroid-delta1-dehydrogenase of rhodococcus rhodochrous: sequencing of the genomic dna and hyperexpression, purification, and characterization of the recombinant enzyme.the gene encoding 3-ketosteroid-delta1-dehydrogenase from rhodococcus rhodochrous was cloned and sequenced. the gene (ksdd) consists of 1,536 nucleotides and encodes an enzyme protein of 511 amino acid residues. the amino terminal methionine residue was deleted in the mature protein. the amino acids involved in the flavin binding site are conserved in the dehydrogenase sequence. the deduced amino acid sequence is highly homologous to that from arthrobacter simplex but less so to that from pseudo ...19989792929
pbla8, from brevibacterium linens, belongs to a gram-positive subfamily of cole2-related plasmids.a 3.1 kb dna fragment from pbla8, a brevibacterium linens cryptic plasmid, containing all the information required for autonomous replication was cloned and sequenced. using deletion analysis, the fragment essential and sufficient for autonomous replication was delimited to 1.5 kb. this fragment is characterized by the presence of an ori site located upstream of an operon encoding two proteins, repa and repb, both essential for replication. based on structural similarities and a strong conservat ...19989802024
septicemia due to arthrobacter species in a neutropenic patient with acute lymphoblastic leukemia. 19989827302
repeated application of carvone-induced bacteria to enhance biodegradation of polychlorinated biphenyls in soil.carvone, the principal component of spearmint oil, induces biodegradation of polychlorinated biphenyls (pcb) by arthrobacter sp. strain b1b. this study investigated the effectiveness of the repeated application of carvone-induced bacteria for bioremediation of aroclor-1242-contaminated soil. control treatments compared a single inoculation of carvone-induced cells, repeated applications of noninduced cells, and repeated applications of cell-free carvone/fructose medium. the results showed that r ...19989830100
detection and isolation of novel rhizopine-catabolizing bacteria from the environmentmicrobial rhizopine-catabolizing (moc) activity was detected in serial dilutions of soil and rhizosphere washes. the activity observed generally ranged between 10(6) and 10(7) catabolic units per g, and the numbers of nonspecific culture-forming units were found to be approximately 10 times higher. a diverse set of 37 isolates was obtained by enrichment on scyllo-inosamine-containing media. however, none of the bacteria that were isolated were found to contain dna sequences homologous to the kno ...19989835587
enhancement of the tolerance of arabidopsis to high temperatures by genetic engineering of the synthesis of glycinebetaine.arabidopsis thaliana was transformed with the coda gene for choline oxidase from arthrobacter globiformis under control of the 35s rna promoter of cauliflower mosaic virus. as a result, high levels of glycinebetaine accumulated in the seeds of transformed plants. transformation with the coda gene significantly enhanced the tolerance to high temperatures during the imbibition and germination of seeds, as well as during growth of young seedlings. the extent of enhancement of the tolerance to high ...19989839462
allergic alveolitis among agricultural workers in eastern poland: a study of twenty cases.the aim of this study was to identify the specific agents which caused extrinsic allergic alveolitis (eaa) in the selected group of 20 agricultural workers from eastern poland. the microbiological analysis of the samples of plant materials or dusts reported by the patients as causing symptoms has been carried out, followed by allergological tests (inhalation challenge, agar-gel precipitation test, inhibition of leukocyte migration, skin test) with extrinsic microbial antigens. it was found that ...19989852490
crystallization of arthrobacter sp. strain 1c n-(1-d-carboxyethyl)-l- norvaline dehydrogenase and its complex with nad+.the novel nad+-linked opine dehydrogenase from a soil isolate arthrobacter sp. strain 1c belongs to an enzyme superfamily whose members exhibit quite diverse substrate specificities. crystals of this opine dehydrogenase, obtained in the presence or absence of co-factor and substrates, have been shown to diffract to beyond 1.8 a resolution. x-ray precession photographs have established that the crystals belong to space group p21212, with cell parameters a = 104.9, b = 80.0, c = 45.5 a and a singl ...19989867431
metabolic engineering of rice leading to biosynthesis of glycinebetaine and tolerance to salt and cold.genetically engineered rice (oryza sativa l.) with the ability to synthesize glycinebetaine was established by introducing the coda gene for choline oxidase from the soil bacterium arthrobacter globiformis. levels of glycinebetaine were as high as 1 and 5 micromol per gram fresh weight of leaves in two types of transgenic plant in which choline oxidase was targeted to the chloroplasts (chlcod plants) and to the cytosol (cytcod plants), respectively. although treatment with 0.15 m nacl [corrected ...19989869407
bacterial degradation of chlorpyrifos in pure cultures and in soil. 19999870989
regulation of lipa gene expression by cell surface proteins in arthrobacter photogonimosexpression of the light-inducible lipa gene in arthrobacter photogonimos by photodynamic compounds and visible light was inhibited by washing cells with 1 m kcl. addition of cell surface extract to kcl-washed cells restored the induction. washing cells with 1 m mgcl2 removed a 14-kda polypeptide and concomitantly caused expression of lipa gene without photodynamic treatment. the purified 14-kda polypeptide inhibited photodynamic induction of lipa gene. these results indicated that regulation of ...19999871106
gene structures and properties of enzymes of the plasmid-encoded nicotine catabolism of arthrobacter nicotinovorans.arthrobacter nicotinovorans is a gram-positive aerobic soil bacterium able to grow on nicotine as its sole source of carbon and nitrogen. the initial steps of nicotine catabolism are catalyzed by nicotine dehydrogenase, the l- and d-specific 6-hydroxynicotine oxidases, and ketone dehydrogenase. the genes encoding these enzymes reside on a 160 kb plasmid, pao1. the cccdna of this plasmid was isolated in high purity and reasonable yield. it served as template material for the construction of a lam ...19989878353
horizontal gene transfer involved in the convergent evolution of the plasmid-encoded enantioselective 6-hydroxynicotine oxidases.the d- and l-specific nicotine oxidases are flavoproteins involved in the oxidative degradation of nicotine by the gram-positive soil bacterium arthrobacter nicotinovorans. their structural genes are located on a 160-kbp plasmid together with those of other nicotine-degrading enzymes. they are structurally unrelated at the dna as well as at the protein level. each of these oxidases possesses a high degree of substrate specificity; their catalytic stereoselectivity is absolute, although they are ...19999929386
purification and characterization of two extracellular proteinases from arthrobacter nicotianae 9458.two extracellular serine proteinases with molecular masses of about 53-55 and 70-72 kda, were purified from arthrobacter nicotianae 9458 and characterized. the enzymes differed with respect to temperature optimum, 55-60 and 37 degrees c, respectively, tolerance to low values of ph and temperature, heat stability, sensitivity to edta and sulfhydryl blocking agents, and hydrophobicity. both proteinases were optimally active in the ph range of 9.0 and 9.5, had considerable activity at ph 6.0 on alp ...19999933928
structural study on a sulfated polysaccharide-peptidoglycan complex produced by arthrobacter sp.the structure of a sulfated polysaccharide-peptidoglycan complex (sp-pg) produced by arthrobacter sp. was analyzed by nmr spectroscopy. in addition, oligosaccharide fragments of the sp-pg-l obtained by hf degradation were analyzed by nmr spectroscopy. these findings indicated that the sulfated polysaccharide (sp) contains a repeating unit composed of two galactofuranosides and a glucopyranoside. the main chain of the trisaccharide is [-->6) beta-d-galf(1-->6)-beta-d-galf(1-->ln, with beta-d-glcp ...19989972234
application of temperature-gradient gel electrophoresis in taxonomy of coryneform bacteria.strains belonging to the gram-positive coryneform soil bacteria were screened genotypically by temperature-gradient gel electrophoresis (tgge). this method allows the sequence-specific separation of amplified fragments of 16s rrna genes. a total of 115 reference strains representing the majority of the species of the genera aeromicrobium, agromyces, arthrobacter, aureobacterium, cellulomonas, curtobacterium, nocardioides and terrabacter were characterized. depending on the genus investigated, th ...199910028252
corynebacterium terpenotabidum sp. nov., a bacterium capable of degrading squalene.the taxonomic status of arthrobacter sp. y-11t, which was described as a squalene-degrading bacterium, was investigated by chemotaxonomic and genetic methods. the strain possesses wall chemotype iv, mk-9(h2) as the predominant menaquinone, mycolic acids, and straight-chain, saturated and monounsaturated fatty acids, with considerable amounts of tuberculostearic acid. the dna g+c content is 67.5 mol%. 16s rrna gene sequence analysis and quantitative dna-dna hybridization experiments provided stro ...199910028267
involvement of two plasmids in the degradation of carbaryl by arthrobacter sp. strain rc100.a bacterium capable of utilizing carbaryl (1-naphthyl n-methylcarbamate) as the sole carbon source was isolated from carbaryl-treated soil. this bacterium was characterized taxonomically as arthrobacter and was designated strain rc100. rc100 hydrolyzes the n-methylcarbamate linkage to 1-naphthol, which was further metabolized via salicylate and gentisate. strain rc100 harbored three plasmids (designated prc1, prc2, and prc3). mutants unable to degrade carbaryl arose at a high frequency after tre ...199910049857
stoichiometry of the topa quinone biogenesis reaction in copper amine oxidases.the stoichiometry of the topa quinone biogenesis reaction in phenylethylamine oxidase from arthrobacter globiformis (agao) has been determined. we have shown that the 6e- oxidation of tyrosine to topa quinone (tpq) consumes 2 mol of o2 and produces 1 mol of h2o2/mol of tpq formed. the rate of h2o2 production is first-order (kobs = 1.0 +/- 0.2 min-1), a rate only slightly lower than the rate of tpq formation directly determined previously (kobs = 1.5 +/- 0.2 min-1). this gives the following net r ...199910074341
removal of nitrate from industrial wastewaters in a pilot plant by nitrate-tolerant klebsiella oxytoca cect 4460 and arthrobacter globiformis cect 4500two strains, a gram-negative bacterium klebsiella oxytoca cect 4460 and a gram-positive, mycelium-forming bacterium arthrobacter globiformis cect 4500, tolerant to up to 1 m nitrate, were isolated from the grounds of a munitions factory. under strict aerobic conditions and with appropriate c-sources, growth of these bacteria took place when the nitrate concentration in the medium was below 150 mm. optimal growth conditions regarding the culture medium composition for the biological removal of ni ...199810099287
cloning, nucleotide sequence and expression of a new l-n-carbamoylase gene from arthrobacter aurescens dsm 3747 in e. coli.an l-n-carbamoyl amino acid amidohydrolase (l-n-carbamoylase) from arthrobacter aurescens dsm 3747 was cloned in e. coli and the nucleotide sequence was determined. after expression of the gene in e. coli the enzyme was purified to homogeneity and characterized. the enzyme was shown to be strictly l-specific and exhibited the highest activity in the hydrolysis of beta-aryl substituted n alpha-carbamoyl-alanines as e.g. n-carbamoyl-tryptophan. carbamoyl derivatives of beta-alanine and charged ali ...199910194852
purification, characterization, and application of an acid urease from arthrobacter mobilis.it has been shown that urea in fermented beverages and foods can serve as a precursor of ethylcarbamate, a potential carcinogen, and acid urease is an effective agent for removing urea in such products. we describe herein the purification and characterization of a novel acid urease from arthrobacter mobilis sam 0752 and show its unique application for the removal of urea from fermented beverages using the japanese rice wine, sake, as an example. the purified acid urease showed an optimum ph for ...199910194859
transformation of cortisone acetate using immobilized arthrobacter simplex cells.a new cell immobilization method was developed in which cells of arthrobacter simplex by-2-3-51 were immobilized by entrapment with a polyvinyl alcohol-alginate complex carrier. the immobilized cells preparation contains spherical beads with high mechanical strength and delta 1-dehydrogenase activity. the activation conditions and enzymatic properties of the immobilized cells were studied. when the initial substrate concentration was 20 mg/ml, the conversion of cortisone acetate to prednisone ac ...199810196636
effects of moisture and sorption on bioavailability of p-hydroxybenzoic acid to arthrobacter sp. in soil.effects of bioavailability on degradation of 14c-p-hydroxybenzoate were examined using sterile soil inoculated with arthrobacter sp. physical accessibility of p-hydroxybenzoate was controlled by varying pore continuity with a range of moisture regimes (-33 to -420 kpa), whereas sorption was controlled via addition of an exchange resin. arthrobacter sp. accessed 94% of p-hydroxybenzoate in soil at -33 kpa, owing to continuity of soil pores and sufficient cells to exploit available space. a deviat ...199910206726
dust-borne bacteria in animal sheds, schools and children's day care centres.a total of 316 bacterial strains, including psychrophiles, mesophiles and thermophiles, were isolated and identified from indoor dusts in schools, children's day care centres and animal sheds. several species which had not previously been reported from indoor environments were found: sphingomonas, brevibacterium, nocardiopsis, deinococcus and rhodococcus/gordona. a new psychrophilic actinomycete genus was also found in animal sheds, representing a new undescribed peptidoglycan type and an unusua ...199910212408
the complete genome sequence of the streptomyces temperate phage straight phic31: evolutionary relationships to other viruses.the completed genome sequence of the temperate streptomyces phage straight phic31 is reported. straight phic31 contains genes that are related by sequence similarities to several other dsdna phages infecting many diverse bacterial hosts, including escherichia, arthrobacter, mycobacterium, rhodobacter, staphylococcus, bacillus, streptococcus, lactobacillus and lactococcus. these observations provide further evidence that dsdna phages from diverse bacterial hosts are related and have had access to ...199910219087
construction and characterization of two recombinant bacteria that grow on ortho- and para-substituted chlorobiphenyls.cloning and expression of the aromatic ring dehalogenation genes in biphenyl-growing, polychlorinated biphenyl (pcb)-cometabolizing comamonas testosteroni vp44 resulted in recombinant pathways allowing growth on ortho- and para-chlorobiphenyls (cbs) as a sole carbon source. the recombinant variants were constructed by transformation of strain vp44 with plasmids carrying specific genes for dehalogenation of chlorobenzoates (cbas). plasmid pe43 carries the pseudomonas aeruginosa 142 ohb genes codi ...199910224015
isolation and primary characterization of an amidase from rhodococcus rhodochrous.amidase (ec 3.5.1.4) was purified to homogeneity from rhodococcus rhodochrous m8 using isopropanol fractionation and exchange chromatography on mono q. the isolated amidase consists of four identical subunits with molecular weight 42+/-2 kd. the activity of the enzyme is maximal at 55-60 degrees c and within the ph range 5-8. the amidase from r. rhodochrous m8 is highly sensitive to such sulfhydryl reagents as hg2+ and cu2+. chelators (edta and o-phenanthroline) and serine proteinase inhibitors ...199910231590
comparison of the fuel oil biodegradation potential of hydrocarbon-assimilating microorganisms isolated from a temperate agricultural soil.strains of hydrocarbon-degrading microorganisms (bacteria and fungi) were isolated from an agricultural soil in france. in a field, a portion was treated with oily cuttings resulting from the drilling of an onshore well. the cuttings which were spread at the rate of 600 g hc m-2 contained 10% of fuel oil hydrocarbons (hc). another part of the field was left untreated. three months after hc spreading, hc adapted bacteria and fungi were isolated at different soil depths in the two plots and identi ...199910231986
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