transformation of cholic acid by arthrobacter simplex.fermentation of cholic acid with arthrobacter simplex (iicb 227) under aerobic conditions yielded 3,12-dioxo-23,24-dinorchola-4,6-dienoic acid, 7 alpha-hydroxy-3,12-dioxo-23,24-dinorchol-4-enoic acid, 3 alpha, 7 alpha-dihydroxy-12-oxo-5 beta-cholan-24-oic acid, 3 alpha, 7 alpha-dihydroxy-12-oxo-5 beta-23,24-dinorcholan-22-oic acid, 7 alpha, 12 alpha-dihydroxy-3-oxo-5 beta-cholan-24-oic acid, 7 alpha-12 alpha-dihydroxy-3-oxo-4-cholenoic acid, 7 alpha, 12 alpha-dihydroxy-3-oxo-23,24-dinorchol-4-en ...19938256259
vermiculin derivatives. part 1: synthesis of vermiculin derivatives.hydrogenation of the macrodiolide antibiotic vermiculin (1) over adams catalyst afforded [8s, 16s]-8,16-bis(2'-oxopropyl)-1,9- dioxyacyclohexadeca-2,5,10,13-tetrone (2), [8s, 16s]-8,16-bis(2'-oxopropyl)-13-hydroxy-1,9-dioxacyclohexadeca- 2,5,10-trione (3), [8s,16s]-8,16-bis(2' oxopropyl)-1,9- dioxacyclohexadeca-2,5,10-trione (4) together with [7s]-4,9-dioxo-7-(4',9'-dioxodecanoyloxy)decanoic acid (5). hydrogenation of diolide 1 over pd/c gave tetrahydrovermiculin (2) only. the prepared compounds ...19938265708
[effect of ozone on airborne microorganisms].the microbicidal effect of ozone in air was tested at concentrations between 50 and 600 micrograms/m3 against the species: staphylococcus epidermidis, micrococcus luteus, arthrobacter citreus, bacillus subtilis (veg.), escherichia coli, salmonella typhimurium, serratia marcescens, pseudomonas fluorescens and candida albicans. the microorganisms were exposed on membrane filters at 60-75% relative humidity and 21.5-22.5 degrees c. after exposure times between 1 min and 60 min, the filters were inc ...19938267833
analysis of low-molecular mass products from biosolubilized coal.a relatively simple, rapid sample preparation method has been developed for analysis of low-molecular mass compounds present in soluble coal products generated by microbial coal solubilizing agents. acidification of the sample followed by direct extraction into hexanes is coupled with gas chromatography/mass spectrometry analysis for characterization of the soluble coal products. characterization of the products can contribute to a more complete understanding of the solubilization processes invo ...19948181700
photoaffinity labeling of acyl-coa oxidase with 12-azidooleoyl-coa and 12-[(4-azidosalicyl)amino]dodecanoyl-coa.synthesis of 32p-labeled coa of high specific activity was achieved using partially purified dephospho-coa kinase (ec from pig liver with [gamma-32p]atp as donor and dephospho-coa as acceptor. a photoaffinity dodecanoic acid analog, 12-[(4-azidosalicyl)amino]dodecanoic acid was synthesized, as were its coa derivative (asd-coa) and the coa derivative of 12-azidooleic acid. the coa derivatives were synthesized from azido fatty acid analogs by acyl-coa synthetase. the synthesized photolab ...19938241127
molecular properties of aminopeptidase ey as a zinc-metalloenzyme.1. aminopeptidase ey from hen's egg yolk contains 1.0 g atom of zinc/mol of a subunit having molecular weight of 150 kda. the inactive, zn(2+)-free apoenzyme was reactivated by co2+, mn2+, ca2+, cd2+, cu2+ and ni2+ in addition to zn2+, whereas mg2+ and fe2+ were ineffective. 2. the enzymatical properties of reconstituted enzymes, except for zn(2+)-reconstituted enzyme, differed from native enzyme. the values for the activation energy were calculated by aminopeptidase ey and co(2+)-reconstituted ...19938288037
three different 2,3-dihydroxybiphenyl-1,2-dioxygenase genes in the gram-positive polychlorobiphenyl-degrading bacterium rhodococcus globerulus p6.rhodococcus globerulus p6 (previously designated acinetobacter sp. strain p6, arthrobacter sp. strain m5, and corynebacterium sp. strain mb1) is able to degrade a wide range of polychlorinated biphenyl (pcb) congeners. the genetic and biochemical analyses of the pcb catabolic pathway reported here have revealed the existence of a pcb gene cluster--bphbc1d--and two further bphc genes--bphc2 and bphc3--that encode three narrow-substrate-specificity enzymes (2,3-dihydroxybiphenyl dioxygenases) that ...19938335622
comparison of the activity of two chondroitin ac lyases on dermatan sulfate. 19938348558
inhibition of sialidases from viral, bacterial and mammalian sources by analogues of 2-deoxy-2,3-didehydro-n-acetylneuraminic acid modified at the c-4 position.the inhibition of sialidase activity from influenza viruses a and b, parainfluenza 2 virus, vibrio cholerae, arthrobacter ureafaciens, clostridium perfringens, and sheep liver by a range of 2-deoxy-2,3-didehydro-n-acetylneuraminic acid analogues modified at the c-4 position has been studied. all substitutions tested resulted in a decrease in the degree of inhibition of the bacterial and mammalian sialidases. for sialidases from influenza viruses a and b, on the other hand, most of the substituti ...19938358225
expression and secretion of an arthrobacter dextranase in the oral bacterium streptococcus gordonii.we have constructed a plasmid to express and secrete dextranase in the oral bacterium streptococcus gordonii. the dextranase gene from arthrobacter sp. strain cb-8 was linked to a promoter and a dna sequence encoding the signal peptide of streptococcus downei glucosyltransferase i (gtfi) followed by the escherichia coli rrnbt1t2 terminator and inserted in the shuttle vector pva838. s. gordonii transformed with this plasmid (pmnk-4) expressed and secreted mature arthrobacter dextranase. the trans ...19938406828
cloning, sequencing, expression, and regulation of the structural gene for the copper/topa quinone-containing methylamine oxidase from arthrobacter strain p1, a gram-positive facultative methylotroph.deoxyoligonucleotides corresponding to amino acid sequences of methylamine oxidase and polyclonal anti-methylamine oxidase antibodies were used to probe arthrobacter strain p1 plasmid and chromosomal dna libraries. two open reading frames, maoxi and maoxii, which are greater than 99% homologous, were cloned from the chromosomal library. the deduced amino acid sequences of the coding regions are identical except for two residues near the c termini. on the other hand, the 5'- and 3'-flanking regio ...19938366046
the penicillin amidase of arthrobacter viscosus (atcc 15294).the nucleotide (nt) sequence of the gene encoding penicillin g amidase (pa) of arthrobacter viscosus strain atcc 15,294 was determined. the sequence contained an open reading frame of 2406 nt with a g+c content of 37%. the deduced amino-acid sequence shows significant homology with other so far identified beta-lactam amidases of gram- bacteria.19948200542
microbial degradation of chlorinated acetophenones.a defined mixed culture, consisting of an arthrobacter sp. and a micrococcus sp. and able to grow with 4-chloroacetophenone as a sole source of carbon and energy, was isolated. 4-chlorophenyl acetate, 4-chlorophenol, and 4-chlorocatechol were identified as metabolites through comparison of retention times and uv spectra with those of standard substances. the proposed pathway was further confirmed by investigation of enzymes. the roles of the two collaborating strains were studied by growth exper ...19938368855
a new lipopeptide biosurfactant produced by arthrobacter sp. strain mis38.a biosurfactant termed arthrofactin produced by arthrobacter species strain mis38 was purified and chemically characterized as 3-hydroxydecanoyl-d-leucyl-d-asparagyl-d-threonyl-d- leucyl-d-leucyl-d-seryl-l-leucyl-d-seryl-l-isoleucyl-l-isoleucyl-l-as paragyl lactone. surface activity of arthrofactin was examined, with surfactin as a control. critical micelle concentration values of arthrofactin and surfactin were around 1.0 x 10(-5) m and 7.0 x 10(-5) m at 25 degrees c, respectively. arthrofactin ...19938407822
inositol-containing lipoglycans in prokaryotes. 19938449324
cloning and expression of the polychlorinated biphenyl-degradation gene cluster from arthrobacter m5 and comparison to analogous genes from gram-negative bacteria.arthrobacter m5 was characterized genetically to determine if the catabolic pathway (controlled by the bph genes), responsible for polychlorinated biphenyl (pcb) biodegradation in this gram-positive strain, was similar to the pathways characterized from various gram-negative bacteria. arthrobacter m5 was originally isolated as a contaminant from a culture of the pcb degrader, acinetobacter sp. strain p6. a bph-specific oligodeoxyribonucleotide (oligo) gene probe (bphc2) was designed by aligning ...19938449411
the m.alui dna-(cytosine c5)-methyltransferase has an unusually large, partially dispensable, variable region.the dna methyltransferase of the alui restriction-modification system, from arthrobacter luteus, converts cytosine to 5-methylcytosine in the sequence agct. the gene for this methyltransferase, aluim, was cloned into escherichia coli and sequenced. a 525-codon open reading frame was found, consistent with deletion evidence, and the deduced amino acid sequence revealed all ten conserved regions common to 5-methylcytosine methyltransferases. the aluim sequence predicts a protein of m(r) 59.0k, in ...19938451189
microbial metabolism of quinoline and related compounds. xvi. quinaldine oxidoreductase from arthrobacter spec. rü 61a: a molybdenum-containing enzyme catalysing the hydroxylation at c-4 of the heterocycle.quinaldine oxidoreductase from arthrobacter spec. rü 61a converts quinaldine to 1h-4-oxoquinaldine. the enzyme was purified 70-fold to apparent homogeneity in a 5-step procedure with a recovery of 4%. the molecular mass of the native enzyme was calculated to be 340,000 da by gel filtration. sds-polyacrylamide gel electrophoresis of the enzyme revealed 3 protein bands corresponding to 82,000 da, 35,000 da and 22,000 da. the enzyme contained 1.6 atoms of molybdenum, 8 atoms of iron, 8 atoms of aci ...19938471177
[arthrobacter proteinase and its effect on fibrin thrombi].arthrobacteria are producers of proteolytic enzymes. optimal cultivation conditions were determined for arthrobacter luteus and the mineral composition of the culture medium providing active synthesis of proteinase was optimized. the proteinase was isolated from the culture liquid of arthrobacter luteus using preparative ion-exchange chromatography on a carboxyl cation exchange resin kmt. the enzyme was homogeneous according to gel chromatography on sephadex g-200 and had a molecular weight of 2 ...19938475025
microbial production of d-malate from produce d-malate from maleate by a microbial reaction, we screened a number of maleate-utilizing microorganisms for enzyme activity by an intact cell system. the strain which showed the best productivity among the 440 strains tested was identified taxonomically as arthrobacter sp. strain mci2612. the optical purity of the malate produced by this strain was 100% d type. the culture and reaction conditions for the production were studied for this strain. addition of amino acids such as l-prolin ...19938476285
morphological, physiological and enzymatic characteristics of cephalosporin acylase-producing arthrobacter strain 45-8a.a bacterial strain producing cephalosporin acylases was isolated from soil. the morphological and physiological properties of this strain suggest that it belongs to the genus arthrobacter, and the isolate was therefore designated arthrobacter strain 45-8a. substrate specificity of the enzyme was examined. the enzyme can convert both cephalosporin acid to 7-aminocephalosporanic acid. an interesting feature of the acylases is their temperature-dependent regulation. activity of acylases was detecte ...19938484708
arthrobacter d-xylose isomerase: protein-engineered subunit interfaces.mutants of arthrobacter d-xylose isomerase were constructed in which one or two disulphide bridges or additional salt bridges were introduced at the a-a* subunit interfaces. these showed no change in enzyme activity or stability compared with the wild-type enzyme. however, a tyr253 mutant in which a disulphide bridge was introduced at the a-b* subunit interface showed reduced thermostability that was identical in both oxidized and reduced forms, and also reduced stability in urea. x-ray-crystall ...19938484737
degradation of a sodium acrylate oligomer by an arthrobacter sp.arthrobacter sp. strain no-18 was first isolated from soil as a bacterium which could degrade the sodium acrylate oligomer and utilize it as the sole source of carbon. when 0.2% (wt/wt) oligomer was added to the culture medium, the acrylate oligomer was found to be degraded by 70 to 80% in 2 weeks, using gel permeation chromatography. to determine the maximum molecular weight for biodegradation, the degradation test was done with the hexamer, heptamer, and octamer, which were separated from the ...19938517751
two-dimensional gel electrophoresis of ribosomal proteins as a novel approach to bacterial taxonomy: application to the genus arthrobacter.ribosomal proteins from 22 strains of 15 different species belong to the genus arthrobacter were analyzed by an improved two-dimensional gel electrophoresis. electrophoretograms of ribosomal proteins from 15 type strains had species-specific patterns. similarity coefficients (sab values) of ribosomal proteins with mol. wt. of greater than about 20,000, among strains of the same species (dna relatedness values of more than 61%) were greater than 0.85, but the sab values among strains of different ...19958520111
characterization of the methylenediphosphonate transport system in arthrobacter sp. glp-1 using the novel tritium-labelled derivative.the novel tritium-labelled derivative of methylenediphosphonate (mdp) was used in uptake studies of arthrobacter sp. glp-1 capable of utilizing a wide range of organophosphonates as its sole source of phosphorus. the mdp uptake was greatly stimulated upon phosphate deprivation. the uptake obeys michaelis-menten kinetics with respective km and vmax values of 33 microm and 0.3 fr.wt. glyphosate and pyrophosphate were competitive inhibitors of mdp uptake. the effect of orthophosphat ...19938428621
cloning and characterization of a gene coding for the catechol 1,2-dioxygenase of arthrobacter sp. ma3.the cata gene, coding for the catechol 1,2-dioxygenase (c12o) of the bacterial strain arthrobacter sp. ma3, was cloned and expressed in escherichia coli. one plasmid containing a 6.1-kb ecori insert was selected by its ability to degrade catechol and to accumulate cis-cis-muconate. the dna insert of this plasmid was mapped with restriction enzymes. the cata gene was subcloned on a 1.3-kb psti-ecori fragment by deleting the adjacent restriction fragments. the nucleotide sequence of cata was deter ...19938423008
arthrobacter d-xylose isomerase: partial proteolysis with thermolysin.the pattern and kinetics of partial proteolysis of arthrobacter d-xylose isomerase tetramer was studied in order to determine the flexibility of surface loops that may control its stability. it was completely resistant to trypsin, chymotrypsin and elastase at 37 degrees c, but thermolysin cleaved specifically and quantitatively at thr-347-leu-348 between helices 10 and 11 to remove 47 residues from the c-terminus of each 43.3 kda subunit. at high temperatures, helices 9 and 10 were removed from ...19938424759
cellular location influences copper-dependent topaquinone formation for phenylethylamine oxidase expressed in escherichia coli k-12.arthrobacter globiformis amine oxidase produced by escherichia coli cells grown in copper-depleted media was reported to undergo activation due to formation of its topaquinone cofactor in a copper-dependent autocatalytic reaction. likewise, a mutated e. coli amine oxidase located in the cytoplasm was reported to form topaquinone autocatalytically in an edta-sensitive reaction. here we show unequivocally that formation of an amine oxidase lacking topaquinone is primarily a consequence of the loca ...19958522142
spectrophotometric detection of topa quinone. 19958524159
resonance raman spectroscopy of quinoproteins. 19958524145
formation of trehalose from maltooligosaccharides by a novel enzymatic system. 19958534970
cloning and nucleotide sequence of the inulin fructotransferase (dfa i-producing) gene of arthrobacter globiformis s14-3.a gene encoding an inulin fructotransferase (dfa i-producing) [ec] from arthrobacter globiformis s14-3 was cloned and the nucleotides sequenced, for the first time. the sequence indicated that the native enzyme protein is composed of 392 amino acid residues. the native enzyme is an extracellar enzyme produced in the culture supernatant of a. globiformis s14-3, but the nucleotide sequence of the gene lacks a sequence for signal peptide for secretion. the 1.5-kb dna fragment encoding the ...19958534968
transformation of synechococcus with a gene for choline oxidase enhances tolerance to salt stress.choline oxidase, isolated from the soil bacterium arthrobacter globiformis, converts choline to glycinebetaine (n-trimethylglycine) without a requirement for any cofactors. the gene for this enzyme, designated coda, was cloned and introduced into the cyanobacterium synechococcus sp. pcc 7942. the coda gene was expressed under the control of a strong constitutive promoter, and the transformed cells accumulated glycinebetaine at intracellular levels of 60-80 mm. consequently the cells acquired tol ...19958555454
development of a specific biotinylated dna probe for the detection of renibacterium salmoninarum.a specific dna probe for the identification of renibacterium salmoninarum, the causative agent of bacterial kidney disease (bkd), was developed from one of 3 clones prs47, prs49, and prs26 of 5.1 kb, 5.3 kb, and 11.3 kb, respectively. the biotinylated prs47/bamhi insert probe was tested on 3 dilutions of dna extracted from 3 strains of r. salmoninarum and from 1 strain each of arthrobacter protophormiae, aeromonas salmonicida, corynebacterium aquaticum, carnobacterium piscicola, listonella angui ...19958548693
isolation and partial characterization of yeast mannan hydrolysing enzymes from bacterial isolates.a range of aerobic, mesophilic bacterial strains capable of producing extracellular alpha-mannanase and alpha-mannosidase enzymes were isolated from various natural habitats using yeast cell wall material as the selective medium. five of them were capable of producing extracellular alpha-mannosidase and alpha-mannanase on yeast mannan as the sole carbon substrate, and they were tentatively identified to the genus level as arthrobacter, streptomyces, pseudomonas, bacillus and acinetobacter. among ...19958577261
manganese(ii)-dependent extradiol-cleaving catechol dioxygenase from arthrobacter globiformis cm-2.a manganese-dependent 3,4-dihydroxyphenylactate 2,3-dioxygenase from arthrobacter globiformis strain cm-2 (mndd) cloned in escherichia coli has been purified to homogeneity. sedimentation equilibrium analysis indicates an alpha 4 homotetrameric holoenzyme structure (4 x 38,861 da). steady-state kinetic analysis of mndd with a variety of substrates and inhibitors yields very similar relative rates to the known fe(ii)- and mn(ii)-dependent 3,4-dihydroxyphenylacetate 2,3-dioxygenases from pseudomon ...19968555170
purification and characterization of the 3-ketosteroid-delta 1-dehydrogenase of arthrobacter simplex produced in streptomyces lividans.the 3-ketosteroid-delta 1-dehydrogenase (ks1dh) gene of arthrobacter simplex ifo12069 cloned in streptomyces lividans was overexpressed, resulting in production of the enzyme both extracellularly and intracellularly. the enzyme was purified by ammonium sulfate fractionation and chromatographies using deae-toyopearl, butyl-toyopearl and toyopearl hw55s from the supernatant of culture broth and cell-free extracts of s. lividans, and both preparations showed the same characteristics. the n-terminal ...19958586617
a pao1-encoded molybdopterin cofactor gene (moaa) of arthrobacter nicotinovorans: characterization and site-directed mutagenesis of the encoded protein.a gene homologous to moaa, the gene responsible for the expression of a protein involved in an early step in the synthesis of the molybdopterin cofactor of escherichia coli, was found to be located 2.7-kb upstream of the nicotine dehydrogenase (ndh) operon on the catabolic plasmid pao1 of arthrobacter nicotinovorans. the moaa protein, containing 354 amino acids, migrated on an sds-polyacrylamide gel with an apparent molecular weight of 40,000, in good agreement with the predicted molecular weigh ...19958588735
formation of polyhydroxylated isoflavones from the soybean seed isoflavones daidzein and glycitein by bacteria isolated from tempe.five tempe-derived bacterial strains identified as micrococcus or arthrobacter species were shown to transform the soybean isoflavones daidzein and glycitein to polyhydroxylated isoflavones by different hydroxylation reactions. all strains converted glycitein and daidzein to 6,7,4'-trihydroxyisoflavone (factor 2) and the latter substrate also to 7,8,4'-trihydroxyisoflavone. three strains transformed daidzein to 7,8,3',4'-tetrahydroxyisoflavone and 6,7,3',4'-tetrahydroxyisoflavone. in addition, t ...19958588745
secretory overproduction of arthrobacter simplex 3-ketosteroid delta 1-dehydrogenase by streptomyces lividans with a multi-copy shuttle vector.the gene for 3-ketosteroid delta 1-dehydrogenase (ksdd) of arthrobacter simplex was expressed in streptomyces lividans and the secreted enzyme was overproduced by using a multi-copy shuttle vector composed of pij702 and puc19. deletional analysis of the recombinant plasmid showed that the entire coding sequence of the ksdd gene was located within a 7-kb segment of the chromosomal dna obtained from the enzyme-producing strain of a. simplex. when s. lividans carrying the recombinant plasmid was gr ...19958590655
genetic characterization of site-specific integration functions of phi aau2 infecting "arthrobacter aureus" c70.all the essential genetic determinants for site-specific integration of corynephage phi aau2 are contained within a 1,756-bp dna fragment, carried on the integrative plasmid p5510, and are shown to be functional in escherichia coli. one open reading frame, orf4, encoding a protein of 266 amino acids was shown to represent the phi aau2 integrase. the nucleotide sequence of the phi aau2 attachment site, attp, and the attb, attl, and attr sequences in the host "arthrobacter aureus" c70 were determi ...19968606175
cloning and sequencing of trehalose biosynthesis genes from arthrobacter sp. q36.a 5.1-kbp genomic dna fragment was cloned from trehalose-producing bacterium arthrobacter sp. strain q36. sequence analysis of the dna fragment revealed two open reading frames of 2325 and 1794 bp, encoding maltooligosyltrehalose synthase (trey) and maltooligosyltrehalose trehalohydrolase (trez), respectively. the 3' end of trey overlaps with the 5' end of trez by one nucleotide, and it is suggested that treyz constitutes and operon. the deduced amino acid sequences of both enzymes have several ...19968605217
distribution of thiols in microorganisms: mycothiol is a major thiol in most actinomycetes.mycothiol [2-(n-acetylcysteinyl)amido-2-deoxy-alpha-d-glucopyranosyl- (1-->1)-myo-inositol] (msh) has recently been identified as a major thiol in a number of actinomycetes (s. sakuda, z.-y. zhou, and y. yamada, biosci. biotech. biochem. 58:1347-1348, 1994; h. s. c. spies and d. j. steenkamp, eur. j. biochem. 224:203-213, 1994; and g. l. newton, c. a. bewley, t. j. dwyer, r. horn, y. aharonowitz, g. cohen, j. davies, d. j. faulkner, and r. c. fahey, eur. j. biochem. 230:821-825, 1995). since thi ...19968606174
sarcosine oxidase contains a novel covalently bound fmn.sarcosine oxidase from corynebacterium sp. p-1 is a heterotetrameric protein containing three different enzymes: noncovalent fad, noncovalent nad+, and covalently bound flavin which is released as 8 alpha-(n3-histidyl)riboflavin upon complete hydrolysis of the protein. the following results show that the covalent flavin is not at the fad level, as previously proposed, but it is rather as 8 alpha-(n3- histidyl)fmn coenzyme. first, no amp is released when the protein moiety is treated with phospho ...19968611516
quinaldine 4-oxidase from arthrobacter sp. rü61a, a versatile procaryotic molybdenum-containing hydroxylase active towards n-containing heterocyclic compounds and aromatic aldehydes.quinaldine 4-oxidase from arthrobacter sp. rü61a, an inducible molybdenum-containing hydroxylase, was purified to homogeneity by an optimized five-step procedure. molecular oxygen is proposed as physiological electron acceptor. electrons are also transferred to artificial electron acceptors with e'o > -8 mv. the molybdo-iron/sulfur flavoprotein regiospecifically attacks its n-heterocyclic substrates: isoquinoline and phthalazine are hydroxylated adjacent to the n-heteroatom at cl, whereas quinal ...19968617260
biosynthesis of topa quinone cofactor in bacterial amine oxidases. solvent origin of c-2 oxygen determined by raman spectroscopy.resonance raman spectroscopy is an excellent technique for providing structural information on the 2,4, 5-trihydroxyphenylalanine quinone (tpq) cofactor in copper-containing amine oxidases. this technique has been used to investigate the copper- and o2-dependent biosynthesis of the tpq cofactor in phenylethylamine oxidase (peao) and histamine oxidase from arthrobacter globiformis. incubation of the holoenzyme in h218o causes frequency shifts at 1684(-26) cm-1 in peao and at 1679(-28) cm-1 in his ...19968617737
comparison of 16s ribosomal rna genes in clavibacter michiganensis subspecies with other coryneform bacteria.nearly complete sequences (97-99%) of the 16s rrna genes were determined for type strains of clavibacter michiganensis subsp. michiganensis, clavibacter michiganensis subsp. insidiosus, clavibacter michiganensis subsp. sepedonicus, and clavibacter michiganensis subsp. nebraskensis. the four subspecies had less than 1% dissimilarity in their 16s rrna genes. comparative studies indicated that the c. michiganensis subsp. shared relatively high homology with the 16s rrna gene of clavibacter xyli. fu ...19958590406
purification and properties of a novel enzyme, maltooligosyl trehalose synthase, from arthrobacter sp. q36.arthrobacter sp. q36 produces a novel enzyme, maltooligosyl trehalose synthase, which catalyzes the conversion of maltooligosaccharide into the non-reducing saccharide, maltooligosyl trehalose (alpha-maltooligosyl alpha-d-glucoside) by intramolecular transglycosylation. the enzyme was purified from a cell-free extract to an electrophoretically homogeneous state by successive column chromatography on sepabeads fp-da13, deae-sephadex a-50, ultrogel aca44, and butyl-toyopearl 650m. the enzyme was s ...19958611744
purification and characterization of a novel enzyme, maltooligosyl trehalose trehalohydrolase, from arthrobacter sp. q36.a novel enzyme, maltooligosyl trehalose trehalohydrolase from arthrobacter sp. q36 was purified from a cell-free extract to an electrophoretically pure state by successive column chromatography on sepabeads fp-da13, butyl-toyopearl 650m, deae-toyopearl 650s, and toyopearl hw-55s. the enzyme specifically catalyzed the hydrolysis of the alpha-1,4-glucosidic linkage that bound the maltooligosyl and trehalose moieties of maltooligosyl trehalose. the km of the enzyme for maltosyl trehalose, maltotrio ...19958611745
cold shock and cold acclimation proteins in the psychrotrophic bacterium arthrobacter globiformis si55.the psychrotrophic bacterium arthrobacter globiformis si55 was grown at 4 and 25 degrees c, and the cell protein contents were analyzed by two-dimensional electrophoresis. cells subjected to cold shocks of increasing magnitude were also analyzed. correspondence analysis of protein appearance distinguished four groups of physiological significance. group i contained cold shock proteins (csps) overexpressed only after a large temperature downshift. group ii contained csps with optimal expression a ...19968655472
moaa of arthrobacter nicotinovorans pao1 involved in mo-pterin cofactor synthesis is an fe-s protein.moaa, involved in an early step in the biosynthesis of the molybdopterin cofactor (moco), has not yet been characterized biochemically and the reaction it catalyzes is unknown. we overexpressed moaa from pao1 of arthrobacter nicotinovorans in escherichia coli as a n-terminal fusion with either glutathione-s-transferase or a 6-histidine tag. the pao1 encoded moaa as well as the fusion proteins functionally complement e. coli moaa mutants. here we show that purified moaa contains approximately 4 m ...19968706892
morphological, physiological, and molecular characterization of actinomycetes isolated from dry soil, rocks, and monument an extended study on the biodiversity of rock-dwelling bacteria, the colony and cell morphology, physiology, protein patterns, and 16s rdna sequences of 17 bacterial strains isolated from different surfaces of rocks, stones, and monuments and from various geographical locations were characterized. all except one strain, which was found to be a bacillus, were members of the order actinomycetales. the majority of the strains either were closely related to geodermatophilus obscurus, which was al ...19968661940
characterization of bacterial communities from activated sludge: culture-dependent numerical identification versus in situ identification using group- and genus-specific rrna-targeted oligonucleotide probesthe structures of bacterial communities were studied in activated sludge samples obtained from the aerobic and anaerobic zones of a wastewater treatment plant showing enhanced phosphorous removal. samples were analyzed by in situ hybridization with oligonucleotide probes complementary to selected regions of the 16s and 23s ribosomal rna (rrna) characteristic for defined phylogenetic entities (genera and larger groups). the microbial community structures revealed by molecular techniques were comp ...19968688004
effects of copper-chrome-arsenate (cca) components on pcp degradation by arthrobacter strain atcc 33790. 19968661907
characterization of novel mono-o-acetylated gm3s containing 9-o-acetyl sialic acid and 6-o-acetyl galactose in equine erythrocytes.novel mono-o-acetylated gm3s, one containing 9-o-acetyl n-glycolyl neuraminic acid and another containing 6'-o-acetyl galactose, were isolated as a mixture from equine erythrocytes, and the structures were characterized by one- and two-dimensional proton nuclear magnetic resonance (nmr) and fast atom bombardment-mass spectrometry (fab-ms). the position of the o-acetyl residue was identified by the downfield shift of the methylene protons at c-9 of n-glycolyl neuraminic acid (9-o-ac gm3) and c-6 ...19968737247
assessment of the biodegradation potential of psychrotrophic microorganisms.bioremediation of polluted temperate and cold temperature environments may require the activity of psychrotrophic bacteria, because their low temperature growth range parallels the ambient temperatures encountered in these environments. in the present study, 135 psychrotrophic microorganisms isolated from a variety of ecosystems in canada were examined for their ability to mineralize 14c-labelled toluene, naphthalene, dodecane, hexadecane, 2-chlorobiphenyl, and pentachlorophenol. a number of the ...19968742353
role of conserved asn-tyr-asp-tyr sequence in bacterial copper/2,4, 5-trihydroxyphenylalanyl quinone-containing histamine oxidase.copper amine oxidase contains a covalently bound quinonoid cofactor, 2,4,5-trihydroxyphenylalanyl quinone (tpq), which is synthesized by post-translational modification of a specific tyrosyl residue occurring in the highly conserved sequence, asn-tyr-(asp/glu)-tyr. to elucidate the role(s) of the conserved sequence in the biogenesis of tpq, each of the corresponding residues at positions 401-404 in the recombinant histamine oxidase from arthrobacter globiformis has been replaced with other amino ...19968798429
microbial biosensor for free fatty acids using an oxygen electrode based on thick film technology.a microbial biosensor based on thick film technology was developed. the microorganisms, arthrobacter nicotianae, were immobilized in ca-alginate directly on the electrode surface. for the stability of the calcium alginate gel the addition of 0.5 mm cacl2 to the assay buffer was necessary. the respiratory activity of the microorganisms was monitored by oxygen consumption at -600 mv vs. ag/agcl reference electrode. the sensor was used in a batch system and was applied to the determination of free ...19968828165
analysis of interaction between the arthrobacter sarcosine oxidase and the coenzyme flavin adenine dinucleotide by site-directed mutagenesis.sarcosine oxidase from arthrobacter sp. te1826 (soxa) tightly binds with the coenzyme flavin adenine dinucleotide (fad). the amino-terminal region of this enzyme was recognized as a part of the fad-binding domain by homology search analysis. comparison with other structurally well-known flavoproteins suggested that the aspartate residue at position 35 (d-35) and the motif sequence (six residues at positions 12 to 17) were important for the interaction with fad. site-directed mutagenesis of each ...19968779579
influence of chromium (vi) and acidic conditions on removal of pentachlorophenol from soil by arthrobacter strain atcc 33790. 19968791548
a phylogenetic study of rubrobacter radiotolerans by sequence analysis of the 16s ribosomal rna gene.partial sequence of the 16s ribosomal rna gene of an extremely high radiotolerant bacterium, rubrobacter radiotolerans (reclassified from arthrobacter radiotolerans by chemical characteristics) was determined by pcr-amplification from a small amount of heat-lysed biomass, followed by direct sequencing of the pcr product. the sequence was aligned with seven species of arthrobacter and also with representatives of various other bacterial groups. r. radiotolerans was confirmed to be out of the arth ...19968843337
cloning and sequencing of a dextranase-encoding cdna from penicillium minioluteum.a cdna from penicillium minioluteum hi-4 encoding a dextranase (1,6-alpha-glucan hydrolase, ec was isolated and characterized. cdna clones corresponding to genes expressed in dextran-induced cultures were identified by differential hybridization. southern hybridization and restriction mapping analysis of selected clones revealed four different groups of cdnas. the dextranase cdna was identified after expressing a cdna fragment from each of the isolated groups of cdna clones in the esch ...19968837470
a phase i clinical and pharmacokinetic study of the angiogenesis inhibitor, tecogalan sodium.tecogalan sodium is an angiogenesis inhibitor isolated from a sulfated polysaccharide produced by the bacterium arthrobacter. the antiangiogenic effect of tecogalan sodium is thought to be mediated by the inhibition of binding of basic fibroblast growth factor to cellular receptors.19968839904
2,4-dioxygenases catalyzing n-heterocyclic-ring cleavage and formation of carbon monoxide. purification and some properties of 1h-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase from arthrobacter sp. rü61a and comparison with 1h-3-hydroxy-4-oxoquinoline 2,4-dioxygenase from pseudomonas putida 33/1.1h-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase (meqdo) was purified from quinaldine-grown arthrobacter sp. rü61a. it was enriched 59-fold in a yield of 22%, and its properties were compared with 1h-3-hydroxy-4-oxoquinoline 2,4-dioxygenase (qdo) purified from pseudomonas putida 33/1. the enzyme-catalyzed conversions were performed in an (18o)o2/(16o)o2 atmosphere. two oxygen atoms of either (18o)o2 or (16o)o2 were incorporated at c2 and c4 of the respective substrates, indicating that these unusual ...19968856057
uptake and utilization of n-octacosane and n-nonacosane by arthrobacter nicotianae kcc b35.arthrobacter nicotianae kcc b35 isolated from blue-green mats densely covering oil sediments along the arabian gulf coast grew well on c10 to c40 n-alkanes as sole sources of carbon and energy. growth on c20 to c40 alkanes was even better than on c10 to c18 alkanes. biomass samples incubated for 6 h with n-octacosane (c28) or n-nonacosane (c29) accumulated these compounds as the predominant constituent alkanes of the cell hydrocarbon fractions. the even chain hexadecane c16 and the odd chain pen ...19968849639
genetic analysis of clavibacter toxicus, the agent of annual ryegrass toxicity.multilocus enzyme electrophoresis was used to examine the relatedness of 52 isolates of clavibacter toxicus, the agent of annual ryegrass toxicity. these included 37 western australian (wa) field isolates sampled in 3 distinct locations over a 2-year period, and 15 isolates sampled from 6 different host plant species in 3 states in australia over approximately 8 years. seventeen reference strains for the related genera curtobacterium, rhodococcus and arthrobacter were examined for comparison. th ...19968870638
the application of serological techniques to the taxonomy of arthrobacter and related organisms.antisera were raised against rods of 17 named arthrobacter and aureobacterium strains. antigenic relationships between these strains, other soil bacteria and new arthrobacter isolates from several soils were studied, using agglutination, immunodiffusion, immunofluorescence and elisa techniques. many of the named arthrobacter species had common antigens, and there were also common antigens amongst named arthrobacter strains and many fresh arthrobacter isolates. agglutination, elisa and immunofluo ...19968868431
transfer of man9glcnac to l-fucose by endo-beta-n-acetylglucosaminidase from arthrobacter protophormiae.we have reported that transglycosylation activity of endo-beta-n-acetylglucosaminidase from arthrobacter protophormiae (endo-a) can be enhanced to near completion using glcnac as an acceptor in a medium containing 30% acetone (fan j-q, takegawa k, iwahara s, kondo a, kato i, abeygunawardana c, lee yc (1995) j biol chem 270: 17723-29). in this paper, we found that the endo-a can also transfer an oligosaccharide, man9glcnac, to l-fuc using man9glcnac2asn as donor substrate in a medium containing 3 ...19968872122
isolation of arthrobacter spp. from clinical specimens and description of arthrobacter cumminsii sp. nov. and arthrobacter woluwensis sp. nov.arthrobacter spp. are very widely distributed in the environment (e.g., soil) but have not been described as causing disease in humans. over a 6-year period, two reference laboratories isolated or received 11 strains which were eventually identified as belonging to the genus arthrobacter. these strains had been initially identified as centers for disease control and prevention coryneform group b-1 and b-3 bacteria (whitishgrayish colonies of 2 mm or greater in diameter after 24 h of incubation, ...19968880479
cloning of the penicillium minioluteum gene encoding dextranase and its expression in pichia pastoris.the dex gene encoding an extracellular dextranase was isolated from the genomic dna library of penicillium minioluteum by hybridization using the dextranase cdna as a probe. comparison of the gene and cdna sequences revealed that the dex gene does not contain introns. amino acid sequences comparison of p. minioluteum dextranase with other reported dextranases reveals a significant homology (29% identity) with a dextranase from arthrobacter sp. cb-8. the dex gene fragment encoding a mature protei ...19968905923
nucleotide sequence and taxonomical distribution of the bacteriocin gene lin cloned from brevibacterium linens m18.linocin m18 is an antilisterial bacteriocin produced by the red smear cheese bacterium brevibacterium linens m18. oligonucleotide probes based on the n-terminal amino acid sequence were used to locate its single copy gene, lin, on the chromosomal dna. the amino acid composition, n-terminal sequence, and molecular mass derived from the nucleotide sequence of an open reading frame of 798 nucleotides coding for 266 amino acids found on a 3-kb bamhi restriction fragment correspond closely to those o ...19968919789
isolation and identification of bacteria from activated sludge purifying petroleum wastewaters.several strains growing well in minimal media with 500 and 1000 mg/l of oil or phenol as a sole carbon source were isolated from activated sludge purifying petroleum waste waters and identified. five of the best growing strains classified as arthrobacter, pseudomonas, xanthomonas and enterobacter were selected and their capacity to remove petroleum components and phenol (in the oil fraction of petrochemical waste waters) was studied. the enzymatic activity of the strains, including respiration i ...19958906933
crystallographic and fluorescence studies of ligand binding to n-carbamoylsarcosine amidohydrolase from arthrobacter sp.crystal structures of n-carbamoylsarcosine amidohydrolase (cshase; ec have been analyzed by x-ray diffraction methods with two different inhibitors bound to the active site at 2.28 a and 2.37 a resolution. the catalytic center of the enzyme could be identified on the basis of these structures. the four substrate binding sites are situated at the intersubunit interfaces of the compact dimers ab and cd of the homotetrameric enzyme. both inhibitors inactivate the enzyme irreversibly throu ...19968913306
biodegradation of 4-chlorophenol via a hydroquinone pathway by arthrobacter ureafaciens cpr706.a newly isolated arthrobacter ureafaciens, strain cpr706, could degrade 4-chlorophenol via a new pathway, in which the chloro-substituent was eliminated in the first step and hydroquinone was produced as a transient intermediate. strain cpr706 exhibited much higher substrate tolerance and degradation rate than other strains that degraded 4-chlorophenol by the hydroxylation at the second carbon position to form chlorocatechol. strain cpr706 could also degrade other para-substituted phenols (4-nit ...19968931337
biodegradation of dimethylsilanediol in soils.the biodegradation potential of [14c]dimethylsilanediol, the monomer unit of polydimethylsiloxane, in soils was investigated. dimethylsilanediol was found to be biodegraded in all of the tested soils, as monitored by the production of 14co2. when 2-propanol was added to the soil as a carbon source in addition to [14c]dimethylsilanediol, the production of 14co2 increased. a method for the selection of primary substrates that support cometabolic degradation of a target compound was developed. by t ...19968953708
endophthalmitis involving an arthrobacter-like organism following intraocular lens implantation. 19968922827
tecogalan sodium inhibits corneal neovascularization induced by basic fibroblast growth factor.the antiangiogenic effect of tecogalan sodium on corneal neovascularization was investigated. tecogalan sodium, a sulfated polysaccharide peptidoglycan complex isolated from an arthrobacter species, has been reported to inhibit angiogenesis induced by basic fibroblast growth factor (bfgf) as well as tumor angiogenesis related to kaposi's sarcoma. corneal neovascularization induced by bfgf was inhibited by tecogalan sodium in a dose-dependent manner. since bfgf is known to have a promoting effect ...19958927305
cloning and sequencing of a cluster of genes encoding novel enzymes of trehalose biosynthesis from thermophilic archaebacterium sulfolobus acidocaldarius.trehalose biosynthesis genes, trez, trex and trey, encoding maltooligosyltrehalose trehalohydrolase (trez), glycogen debranching enzyme (trex), and maltooligosyltrehalose synthase (trey) have been cloned from the thermophilic archaebacterium sulfolobus acidocaldarius atcc33909. the amino-acid sequences deduced from trez, trex and trey are composed of 556, 713 and 720 amino-acid residues, respectively. trez and trey are 33-40% homologous to the corresponding enzymes from arthrobacter sp. q36. we ...19968980629
ym-30059, a novel quinolone antibiotic produced by arthrobacter sp. 19968823519
the action in vivo of glycine betaine in enhancement of tolerance of synechococcus sp. strain pcc 7942 to low temperature.the cyanobacterium synechococcus sp. strain pcc 7942 was transformed with the coda gene for choline oxidase from arthrobacter globiformis under the control of a constitutive promoter. this transformation allowed the cyanobacterial cells to accumulate glycine betaine at 60 to 80 mm in the cytoplasm. the transformed cells could grow at 20 degrees c, the temperature at which the growth of control cells was markedly suppressed. photosynthesis of the transformed cells at 20 degrees c was more toleran ...19978990284
characterization of catechol catabolic genes from rhodococcus erythropolis 1cp.the biochemical characterization of the muconate and the chloromuconate cycloisomerases of the chlorophenol-utilizing rhodococcus erythropolis strain 1cp previously indicated that efficient chloromuconate conversion among the gram-positive bacteria might have evolved independently of that among gram-negative bacteria. based on sequences of the n terminus and of tryptic peptides of the muconate cycloisomerase, a fragment of the corresponding gene has now been amplified and used as a probe for the ...19978990288
flagellar proteins of motile spores of actinomycetes.flagella of some of the actinoplanete genera were purified and the molecular sizes of their flagellin subunits compared by sds-page analysis to flagellins of cells of other bacteria. several species of actinoplanes have a major flagellar protein of subunit sizes of 42-43 kda and a lesser amount of a second protein, possibly a minor flagellin subunit, of 60 kda. the flagellar protein sizes of other actinoplanetes ranged from 32-43 kda (major) and 48-58 kda (minor). antibodies formed against the 4 ...19968987497
biodegradability of oxidized poly(vinyl alcohol).poly(vinyl alcohol) dehydrogenase (pvadh) purified from pseudomonas sp. 113p3 catalyzed an oxidation of poly(vinyl alcohol) (pva) in the presence of pyrroloquinoline quinone (pqq) to give a beta-diketone structure on pva. although pvadh oxidized not only enzymatically oxidized pva but also chemically oxidized pva, pva-degrading microorganisms, pseudomonas sp. 113p3 and arthrobacter tumescens sp. 52-1 grew on the enzymatically oxidized pva, but not on the chemically oxidized pva. this suggests th ...19968987864
clinical microbiology of coryneform bacteria.coryneform bacteria are aerobically growing, asporogenous, non-partially-acid-fast, gram-positive rods of irregular morphology. within the last few years, there has been a massive increase in the number of publications related to all aspects of their clinical microbiology. clinical microbiologists are often confronted with making identifications within this heterogeneous group as well as with considerations of the clinical significance of such isolates. this review provides comprehensive informa ...19978993861
cloning, sequencing, and expression of arthrobacter protophormiae endo-beta-n-acetylglucosaminidase in escherichia coli.the gene encoding endo-beta-n-acetylglucosaminidase from arthrobacter protophormiae (endo-a) was cloned, and its nucleotide sequence was determined. a single open reading frame consisting of 1935 base pairs and encoding a polypeptide composed of signal peptides of 24 amino acids and a mature protein of 621 amino acids was found. the primary structure of endo-a exhibited significant homology with fo1f.10 gene product from caenorhabditis elegans and weak homology with peptide-n4-(n-acetyl-beta-d-g ...19979015383
molecular cloning of an inulin fructotransferase (depolymerizing) gene from arthrobacter sp. h65-7 and its expression in escherichia coli.the gene encoding an extracellular inulin fructotransferase (depolymerizing) (inulase ii) (ec, designated ift gene, was cloned from the genomic dna of arthrobacter sp. h65-7, and expressed in escherichia coli for the first time. sequence analysis showed a single open reading frame consisting of 1314 base pairs that encoded a signal peptide of 32 amino acids and a mature protein of 405 amino acids. the primary structure showed a homology of 49.8% with that of the inulin fructotransferas ...19979028036
classification of catechol 1,2-dioxygenase family: sequence analysis of a gene for the catechol 1,2-dioxygenase showing high specificity for methylcatechols from gram+ aniline-assimilating rhodococcus erythropolis an-13.gram+ aniline-assimilating rhodococcus erythropolis an-13 (an-13) produces catechol 1,2-dioxygenase (c12o) showing high enzymatic activities for 3- and 4-methylcatechols [aoki et al. (1984) agric. biol. chem. 48, 2087-2095]. a 3.0 kb sau3ai fragment carrying a gene encoding c12o(cata) was cloned by selection of transformants showing c12o activity from a gene library of an-13. furthermore, we specified a 1.6 kb sali fragment containing cata from the sau3ai fragment by subcloning. sequence analysi ...19979034312
biodegradation of methyl t-butyl ether by pure bacterial cultures.three pure bacterial cultures degrading methyl t-butyl ether (mtbe) were isolated from activated sludge and fruit of the gingko tree. they have been classified as belonging to the genuses methylobacterium, rhodococcus, and arthrobacter. these cultures degraded 60 ppm mtbe in 1-2 weeks of incubation at 23-25 degrees c. the growth of the isolates on mtbe as sole carbon source is very slow compared with growth on nutrient-rich medium. uniformly-labeled [14c]mtbe was used to determine 14co2 evolutio ...19979035411
catalysis by a new sialidase, deaminoneuraminic acid residue-cleaving enzyme (kdnase sm), initially forms a less stable alpha-anomer of 3-deoxy-d-glycero-d-galacto-nonulosonic acid and is strongly inhibited by the transition state analogue, 2-deoxy-2, 3-didehydro-d-glycero-d-galacto-2-nonulopyranosonic acid, but not by 2-deoxy-2,3-didehydro-n-acetylneuraminic acid.deaminoneuraminic acid residue-cleaving enzyme (kdnase sm) is a new sialidase that has been induced and purified from sphingobacterium multivorum. catalysis by this new sialidase has been studied by enzyme kinetics and 1h nmr spectroscopy. vmax/km values determined for synthetic and natural substrates of kdnase sm reveal that 4-methylumbelliferyl-kdn (kdnalpha2meumb, vmax/km = 0.033 min-1) is the best substrate for this sialidase, presumably because of its good leaving group properties. the tran ...19979038146
structures of sialylated o-linked oligosaccharides of bovine peripheral nerve alpha-dystroglycan. the role of a novel o-mannosyl-type oligosaccharide in the binding of alpha-dystroglycan with laminin.alpha-dystroglycan is a heavily glycosylated protein, which is localized on the schwann cell membrane as well as the sarcolemma, and links the transmembrane protein beta-dystroglycan to laminin in the extracellular matrix. we have shown previously that sialidase treatment, but not n-glycanase treatment, of bovine peripheral nerve alpha-dystroglycan greatly reduces its binding activity to laminin, suggesting that the sialic acid of o-glycosidically-linked oligosaccharides may be essential for thi ...19978999917
expression of sulfated gangliosides in the central nervous system.several sulfated lipids were detected in the ganglioside fraction isolated from a cell line of oligodendrocyte progenitors that had been metabolically labeled with [35s] sulfate. separation of the ganglioside fraction by two-dimensional tlc showed that, except for galactosylceramide-sulfate, none of the sulfate-labeled lipids comigrated with those glycosphingolipids visualized by orcinol staining, indicating that these sulfolipids were quantitatively minor components. at least eight sulfate-labe ...19979003081
pcp degradation is mediated by closely related strains of the genus sphingomonas.there have been numerous reports in the literature of diverse bacteria capable of degrading pentachlorophenol (pcp). in order to gain further insight into the phylogenetic relationships of pcp-degrading bacteria, we examined four strains: arthrobacter sp. strain atcc 33790, flavobacterium sp. strain atcc 39723, pseudomonas sp. strain sr3, and sphingomonas sp. strain ra2. these organisms were isolated from different geographical locations and all of them degrade high concentrations (100-200 mg/l) ...19979004518
identification of yeasts and coryneform bacteria from the surface microflora of brick cheeses.coryneform bacteria and yeasts of 21 brick cheeses from six german dairies, produced by using undefined ripening cultures, were identified. arthrobacter nicotianae, brevibacterium linens, corynebacterium ammoniagenes, corynebacterium variabilis and rhodococcus fascians were found in significant numbers. out of 148 coryneform isolates 36 could not be identified at the species level. with the exception of a large rennet cheese, the coryneform microflora of rennet and acid cured cheeses were simila ...19979039559
new metabolites in the degradation of fluorene by arthrobacter sp. strain f101.identification of new metabolites and demonstration of key enzyme activities support and extend the pathways previously reported for fluorene metabolism by arthrobacter sp. strain f101. washed-cell suspensions of strain f101 with fluorene accumulated 9-fluorenone, 4-hydroxy-9-fluorenone, 3-hydroxy-1-indanone, 1-indanone, 2-indanone, 3-(2-hydroxyphenyl) propionate, and a compound tentatively identified as a formyl indanone. incubations with 2-indanone produced 3-isochromanone. the growth yield wi ...19979055403
distribution of amine oxidases and amine dehydrogenases in bacteria grown on primary amines and characterization of the amine oxidase from klebsiella oxytoca.the bacteria klebsiella oxytoca lmd 72.65 (atcc 8724), arthrobacter p1 lmd 81.60 (ncib 11625), paracoccus versutus lmd 80.62 (atcc 25364), escherichia coli w lmd 50.28 (atcc 9637), e. coli k12 lmd 93.68, pseudomonas aeruginosa pao1 lmd 89.1 (atcc 17933) and pseudomonas putida lmd 68.20 (atcc 12633) utilized primary amines as a carbon and energy source, although the range of amines accepted varied from organism to organism. the gram-negative bacteria k. oxytoca and e. coli as well as the gram-pos ...19979043125
mechanistic studies of topa quinone biogenesis in phenylethylamine alternative purification for apophenylethylamine oxidase from arthrobacter globiformis has been developed, which avoids the use of possible contaminants that may interfere with the topa quinone (tpq) self-processing reaction. the binding of cu(ii) and the kinetics of tpq formation in these enzyme preparations have been reinvestigated. our results show that cu(ii) is not significantly reduced when added to the apoprotein under anaerobic conditions. the cu(ii) epr and circular dichroism spectra ...19979047291
manganese(ii) active site mutants of 3,4-dihydroxyphenylacetate 2,3-dioxygenase from arthrobacter globiformis strain cm-2.whereas all other members of the extradiol-cleaving catechol dioxygenase family are iron-dependent, the 3,4-dihydroxyphenylacetate 2,3-dioxygenase (mndd) from arthrobacter globiformis cm-2 is dependent on manganese for catalytic activity. recently, the endogenous iron ligands of one family member, the 2,3-dihydroxybiphenyl 1,2-dioxygenase (bphc), were identified crystallographically as two histidines and a glutamic acid [sugiyama, k., et al. (1995) proc. jpn. acad., ser. b 71, 32-35; han, et al. ...19979047314
is1473, a putative insertion sequence identified in the plasmid pao1 from arthrobacter nicotinovorans: isolation, characterization, and distribution among arthrobacter species.a putative insertion sequence (is1473) has been cloned and sequenced. the 1087-bp element was found between the moaa and the ndha genes in the upstream region of the nicotine dehydrogenase (ndh) operon in the 160-kb pao1 plasmid of arthrobacter nicotinovorans. it is flanked by an imperfect repeat of 33 bp and carries two overlapping open reading frames which, by programmed -1 translational frameshifting, may produce a transposase of 36.735 da with a pi = 10. 18. the deduced protein is similar to ...19979073580
hydroxylamine oxidation in heterotrophic nitrate-reducing soil bacteria and purification of a hydroxylamine-cytochrome c oxidoreductase from a pseudomonas species.hydroxylamine oxidation was measured in four recently isolated heterotrophic nitrate-reducing bacteria belonging to the genera pseudomonas, moraxella, arthrobacter and aeromonas. a hydroxylamine-cytochrome c oxidoreductase activity was detected in periplasmic fractions of the pseudomonas and aeromonas spp. and in total soluble fractions of the arthrobacter sp. a monomeric 19-kda non-haem iron hydroxylamine-cytochrome c oxidoreductase was purified from the pseudomonas species and shown to be simi ...19969082922
species-specific beta-n-acetylgalactosaminylation of serum igg proteins.lectin blot analysis of bovine, goat, human, rabbit and mouse serum immunoglobulin g (igg) samples revealed that wisteria floribunda agglutinin (wfa) binds to the heavy chains of bovine, goat and human serum igg proteins but not those of the rabbit and mouse proteins. wfa-positive light chain bands were also detected in bovine, goat and human serum igg samples only after the filters were treated with arthrobacter ureafaciens sialidase. the wfa-binding to these igg proteins was abolished by treat ...19979101715
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