Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| enzymatic dehalogenation of pentachlorophenol by extracts from arthrobacter sp. strain atcc 33790. | arthrobacter sp. strain atcc 33790 was grown with pentachlorophenol (pcp) as the sole source of carbon and energy. crude extracts, which were prepared by disruption of the bacteria with a french pressure cell, showed no dehalogenating activity with pcp as the substrate. after sucrose density ultracentrifugation of the crude extract at 145,000 x g, various layers were found in the gradient. one yellow layer showed enzymatic conversion of pcp. one chloride ion was released per molecule of pcp. the ... | 1989 | 2793827 |
| comparison of human, simian, and bovine rotaviruses for requirement of sialic acid in hemagglutination and cell adsorption. | human rotaviruses (wa, kun, mo) showed hemagglutination (ha) only with fixed 1-day-old chicken erythrocytes, and their ha activities were completely destroyed by trypsin activation of virions. simian sa-11 and bovine ncdv had ha activities not only against fixed erythrocytes but also against fresh erythrocytes from various species. their ha activities against fixed erythrocytes were also inhibited by trypsin activation, but those against fresh erythrocytes were not. neuraminidase treatment of fi ... | 1989 | 2549710 |
| biodegradation of crude oils. | petroleum from well sites in the gifhorn trough (lower saxony, nw-germany) and the maracaibo basin (venezuela) contained various types of microorganisms capable of degrading crude oils. genetically related oils were inoculated with the isolated microorganisms and the degradation of the oils was followed by chromatographic techniques. parameters important for the reactions (ph, supply of oxygen, nitrogen and phosphorus, reaction medium) were monitored and optimized. the degradation of n-alkanes w ... | 1989 | 2727641 |
| a new nad+-dependent opine dehydrogenase from arthrobacter sp. strain 1c. | a new nad+-dependent opine dehydrogenase was purified to homogeneity from arthrobacter sp. strain 1c isolated from soil by an enrichment culture technique. the enzyme has a molecular weight of about 70,000 and consists of two identical subunits with molecular weights of about 36,000. the enzyme catalyzed a reversible oxidation-reduction reaction of opine-type secondary amine dicarboxylic acids. in the oxidative deamination reaction, the enzyme was active toward unusual opines, such as n-[1-r-(ca ... | 1989 | 2753861 |
| expression of the arthrobacter viscosus penicillin g acylase gene in escherichia coli and bacillus subtilis. | the penicillin g acylase gene cloned from arthrobacter viscosus 8895gu was subcloned into vectors, and the recombinant plasmids were transferred into escherichia coli or bacillus subtilis. both e. coli and b. subtilis transformants expressed the a. viscosus penicillin g acylase. the enzyme activity was found in the intracellular portion of the e. coli transformants or in the cultured medium of the b. subtilis transformants. penicillin g acylase production in the b. subtilis transformants was 7.2 ... | 1989 | 2504107 |
| [hydrophilic-hydrophobic properties of microorganisms under various culturing conditions]. | the index of hydrophobicity and the hydrophilic-hydrophobic properties of seven microbial cultures were determined by studying their adhesion to n-hexadecane. the index of hydrophobicity was shown to be a stable characteristic of a culture grown in media with different carbon sources. as was found for escherichia coli k-12 and acinetobacter calcoaceticus k-9 whose hydrophobicity indicates were quite different, the character of cell hydration was virtually independent of the growth phase and did ... | 1989 | 2511395 |
| binding of fad to 6-hydroxy-d-nicotine oxidase apoenzyme prevents degradation of the holoenzyme. | expression of the 6-hydroxy-d-nicotine oxidase (6-hdno) gene from arthrobacter oxidans cloned into escherichia coli showed a marked temperature-dependence. transformed e. coli cells grown at 30 degrees c exhibited a several-fold higher 6-hdno activity than did cells grown at 37 degrees c. this effect did not depend on the promoter used for expression of the cloned gene in e. coli, nor was it an effect of 6-hdno mrna instability at 37 degrees c. studies performed in vivo and in vitro revealed tha ... | 1989 | 2649085 |
| crystallisation and preliminary analysis of glucose isomerase from streptomyces albus. | the glucose isomerase of streptomyces albus has been crystallised from a dilute solution of magnesium chloride buffered at a ph of 6.8-7.0. the crystals are in the space group i222 with cell dimensions a = 93.9 a, b = 99.5 a and c = 102.9 a. there is one monomer of the tetrameric molecule per asymmetric unit of the crystal and the packing density is 2.93 a3.da-1. the tetramer sits on the 222 symmetry point of the crystal. native data have been recorded to a resolution of 1.9 a and the crystals d ... | 1989 | 2651156 |
| covalent cofactor binding to flavoenzymes requires specific effectors. | modification by covalent fad attachment to a histidine residue via an 8 alpha-(n3-histidyl)-riboflavin linkage occurs in several flavoenzymes. among them is 6-hydroxy-d-nicotine oxidase (6-hdno) of arthrobacter oxidans and the flavoprotein subunits of the fumarate reductase and succinate dehydrogenase complex of escherichia coli and other bacterial and eukaryotic cells. we found that 6-hdno holoenzyme formation from apo-6-hdno, monitored by [14c]fad incorporation and increase in enzyme activity, ... | 1989 | 2659351 |
| effect of ph and inoculum size on phenol degradation by bacteria isolated from landfill waste. | four aerobic species of mesophilic bacteria which catabolise phenol as the sole carbon source were isolated from landfill waste, and identified as acinetobacter, arthrobacter, micrococcus and nocardia spp. in vitro studies in defined medium revealed variations in abilities of the isolates to catabolise phenol, and in ph growth optima ranging from 6.8 to 7.6. the ph of culture media decreased with growth, and phenol catabolism was impeded below ph 5.4. phenol catabolism in landfill leachate occur ... | 1989 | 15092404 |
| induction and purification of endo-beta-n-acetylglucosaminidase from arthrobacter protophormiae grown in ovalbumin. | arthrobacter protophormiae produced a high level of extracellular endo-beta-n-acetylglucosaminidase when cells were grown in a medium containing ovalbumin. the enzyme was induced by the glycopeptide fraction of ovalbumin prepared by pronase digestion. production of the enzyme was also induced by glycoproteins such as yeast invertase and bovine ribonuclease b but not by monosaccharides such as mannose, n-acetylglucosamine, and galactose. the enzyme was purified to homogeneity as demonstrated by p ... | 1989 | 16348072 |
| isolation and preliminary characterization of hydroxamic acids formed by nitrogen-fixing azotobacter chroococcum b-8. | the free-living diazotroph azotobacter chroococcum b-8 responded to iron-limited growth conditions by forming hydroxamic acids and an 85,000-dalton outer membrane protein. the fe(iii)-binding hydroxamate compounds stimulated the growth of arthrobacter flavescens jg-9 and gave a positive csaky reaction for bound hydroxylamines. the hydroxamates were isolated from liquid cultures by benzyl alcohol extraction and purified by size exclusion chromatography and high-performance liquid chromatography. ... | 1989 | 16347843 |
| degradation of 4-chlorobenzoate by facultatively alkalophilic arthrobacter sp. strain sb8. | a facultative alkalophile capable of utilizing 4-chlorobenzoate (4-cba), strain sb8, was isolated from soil with an alkaline medium (ph 10.0) containing the haloaromatic compound as the carbon source. the strain, identified as an arthrobacter sp., showed rather extensive 4-cba-degrading ability. 4-cba utilization by the strain was possible in the alkaline medium containing up to 10 g of the compound per liter. the 4-cba-dechlorinating activity of resting cells was almost completely uninhibited b ... | 1989 | 16347854 |
| protozoan response to the addition of bacterial predators and other bacteria to soil. | representatives of several categories of bacteria were added to soil to determine which of them might elicit responses from the soil protozoa. the various categories were nonobligate bacterial predators of bacteria, prey bacteria for these predators, indigenous bacteria that are normally present in high numbers in soil, and non-native bacteria that often find their way in large numbers into soil. the soil was incubated and the responses of the indigenous protozoa were determined by most-probable ... | 1989 | 16347983 |
| kinetic model of hydrocortisone 1-en-dehydrogenation by arthrobacter globiformis. | a kinetic model of the hydrocortisone-to-prednisolone transformation by arthrobacter globiformis is constructed using the experimental data obtained in studies of this process. besides adequately describing experimental data, the model allows one to determine the relation between hydrocortisone oxidation and the level of endogenous substrates in bacterial cells, and the relation between the saturating concentration of hydrocortisone in the enzymic system of bacteria and the content of endogenous ... | 1989 | 18587966 |
| kinetic model for the regulation of redox reaction in steroid transformation by arthrobacter globiformis cells. | a kinetic model of hydrocortisone transformation was developed in studies of the kinetics of biochemical systems. the regulatory bases of the model are the biosynthesis of steroid-transforming enzymes and their activity, the level of endogenous substrates, the respiratory chain activity, and the initial concentrations of reagents. when compared, the experimental data completely coincide with the results of the computer modeling, the coincidence being not only qualitative but also quantitative. i ... | 1989 | 18587967 |
| immobilization of arthrobacter simplex in a thermally reversible hydrogel: effect of temperature cycling on steroid conversion. | arthrobacter simplex cells, which convert the steroid hydrocortisone to prednisolone, have been entrapped in a thermally reversible hydrogel. such hydrogels exhibit a lower critical solution temperature (lcst) wherein the gel shrinks and deswells when it is warmed through its lcst, and then reversibly expands and reswells when it is cooled below the lcst. the immobilized cell-hydrogel system has been thermally cycled between two temperatures, each below the lcst. the upper temperature was select ... | 1990 | 18592505 |
| adhesion of an amylolytic arthrobacter sp. to starch-containing plastic films. | cells of the amylolytic bacterium kb-1 (thought to be an arthrobacter sp.) adhered ( approximately 70%) to the surface of plastic films composed of starch-poly (methylacrylate) graft copolymer (starch-pma), but did not adhere (<10%) to films composed of polymethylacrylate (pma), polyethylene (pe), carboxymethyl cellulose, or a mixture of pe plus poly (ethylene-coacrylic acid) (eaa), starch plus pe, or starch plus pe and eaa. about 30% of the cells adhered to gelatinized insoluble starch. dithiot ... | 1990 | 16348173 |
| microbial metabolism of tholin. | in this paper, we show that a wide variety of common soil bacteria are able to obtain their carbon and energy needs from tholin (a class of complex organic heteropolymers thought to be widely distributed through the solar system; in this case tholin was produced by passage of electrical discharge through a mixture of methane, ammonia, and water vapor). we have isolated aerobic, anaerobic, and facultatively anaerobic bacteria which are able to use tholin as a sole carbon source. organisms which ... | 1990 | 11538367 |
| different types of dienelactone hydrolase in 4-fluorobenzoate-utilizing bacteria. | of various benzoate-utilizing bacteria tested, alcaligenes eutrophus 335, a. eutrophus h16, a. eutrophus jmp222, a. eutrophus jmp134, alcaligenes strain a7, and pseudomonas cepacia were able to grow with 4-fluorobenzoate as the sole source of carbon and energy. p. cepacia also utilizes 3-fluorobenzoate. except for a. eutrophus jmp134, which is known to grow with 2,4-dichlorophenoxyacetate and 3-chlorobenzoate (r. h. don and j. m. pemberton, j. bacteriol. 145:681-686, 1981), the strains were unab ... | 1990 | 2394679 |
| chlorinated biphenyl mineralization by individual populations and consortia of freshwater bacteria. | comparative studies were performed to investigate the contribution of microbial consortia, individual microbial populations, and specific plasmids to chlorinated biphenyl biodegradation among microbial communities from a polychlorinated biphenyl-contaminated freshwater environment. a bacterial consortium, designated lps10, was shown to mineralize 4-chlorobiphenyl (4cb) and dehalogenate 4,4'-dichlorobiphenyl. the lps10 consortium involved three isolates: pseudomonas testosteroni (lps10a), which m ... | 1990 | 2117875 |
| new ganglioside analogs that inhibit influenza virus sialidase. | synthetic thioglycoside-analogs of gangliosides such as neu5ac alpha(2-s-6)glc beta(1-1)ceramide (1) and the gm3 analog neu5ac alpha(2-s-6)gal beta(1-4)glc beta(1-1)ceramide (2), competitively inhibited gm3 hydrolysis by the sialidase of different subtypes of human and animal influenza viruses with an apparent ki value of 2.8 x 10(-6) and 1.5 x 10(-5) m, respectively. the inhibitory activity of the ganglioside gm4 analog [neu5ac alpha(2-s-6)gal beta(1-1)ceramide (3)], in which the glucose of 1 w ... | 1990 | 2136350 |
| methylamine oxidase from arthrobacter p1 as a prototype of eukaryotic plasma amine oxidase and diamine oxidase. | methylamine oxidase (maox) from gram-positive soil bacterium arthrobacter p1 catalyzes the oxidation of ch3nh2 to h2c = o and nh4+ via reduction of o2 to h2o2. past work indicates that maox is similar to mammalian plasma amine oxidase (pao) and diamine oxidase (dao), plant dao, and yeast peroxisomal amine oxidase (yao). all have mr congruent to 170,000 and are composed of 2 identical subunits, each of which contains 1 atom of cu(ii) and one molecule of quinonoid cofactor. herein, we report furth ... | 1990 | 1965196 |
| an examination of proton translocation and energy conservation during heterotrophic nitrification. | whether selected heterotrophic nitrifiers, as do the autotrophs, conserve energy during the oxidation of their nitrogenous substrates was studied. the examination of proton translocation of four different bacterial nitrifiers capable of pyruvic oxime [(po), ch3-c(noh)-cooh] nitrification and by an nh4+ oxidizing arthrobacter sp. was initiated. three of the po nitrifying bacteria, all pseudomonads, oxidize hydroxylamine (nh2oh) at a greater rate than po and yielded only stoichiometric protons whe ... | 1990 | 2157622 |
| structural study of cell wall phosphomannan of candida albicans nih b-792 (serotype b) strain, with special reference to 1h and 13c nmr analyses of acid-labile oligomannosyl residues. | chemical structures of manno-oligosaccharides, from biose to heptaose, released from the phosphomannan of candida albicans nih b-792 strain (serotype b) by mild acid hydrolysis were investigated. the results of 1h nmr, 13c nmr, and fast atom bombardment mass spectrometry analyses confirmed that these manno-oligosaccharides belong to a homologous beta-1,2-linked series. although chemical shifts of 1h nmr patterns of these oligosaccharides were considerably too complicated to be assigned, their 13 ... | 1990 | 2181936 |
| soluble and cell-associated haemagglutinins of helicobacter (campylobacter) pylori. | some plate-grown strains of helicobacter (campylobacter) pylori that were harvested into phosphate-buffered saline and left for 1 h released soluble haemagglutinins. these caused high-titre agglutination of human and guinea-pig erythrocytes, whereas chicken, sheep and bovine erythrocytes were agglutinated at various titres. six of 10 strains which had been subcultured repeatedly did not possess soluble haemagglutinins. slide agglutination of bacterial suspensions demarcated the strains into two ... | 1990 | 2258915 |
| utilization of acrylonitrile by bacteria isolated from petrochemical waste waters. | a bacterium, utilising acrylonitrile as a sole source of carbon and nitrogen, was isolated from indian petrochemical corporation limited (ipcl) waste waters and identified as arthrobacter sp. this strain could also utilize acetonitrile, acetamide and acrylamide individually as a source of carbon and nitrogen. the metabolic studies with the whole cells indicated the sequential conversion of the nitrile to the respective amide and then to the respective acid and ammonia. the rate of nitrile hydrol ... | 1990 | 2279769 |
| observations of reaction intermediates and the mechanism of aldose-ketose interconversion by d-xylose isomerase. | crystallographic studies of d-xylose isomerase (d-xylose ketol-isomerase, ec 5.3.1.5) incubated to equilibrium with substrate/product mixtures of xylose and xylulose show electron density for a bound intermediate. the accumulation of this bound intermediate shows that the mechanism is a non-michaelis type. carrell et al. [carrell, h. l., glusker, j. p., burger, v., manfre, f., tritsch, d. & biellmann, j.-f. (1989) proc. natl. acad. sci. usa 86, 4440-4444] and the present authors studied crystals ... | 1990 | 2304904 |
| mechanism for aldose-ketose interconversion by d-xylose isomerase involving ring opening followed by a 1,2-hydride shift. | the active site and mechanism of d-xylose isomerase have been probed by determination of the crystal structures of the enzyme bound to various substrates, inhibitors and cations. ring-opening is an obligatory first step of the reaction and is believed to be the rate-determining step for the aldose to ketose conversion. the structure of a complex with a cyclic thio-glucose has been determined and it is concluded that this is an analogue of the michaelis complex. at -10 degrees c substrates in cry ... | 1990 | 2319597 |
| rapid detection of extended ganglio-series gangliosides with terminal galnac beta 1-4gal sequence on high performance thin layer chromatography plates. | the mouse monoclonal antibody 2d4, which recognizes the terminal galnac beta 1-4gal-disaccharide of ggose3cer and ggose5cer, was used for the detection of ganglio-series gangliosides. the method involves separation of gangliosides on thin layer chromatography plates, followed by silica gel fixation, arthrobacter ureafaciens neuraminidase treatment and final immunostaining of desialylated gangliosides with the monoclonal antibody 2d4. both neuraminidase and the hybridoma 2d4 producing the specifi ... | 1990 | 2350601 |
| characterization of phi ga1, an inducible phage particle from brevibacterium flavum. | eighteen related strains of arthrobacter, brevibacterium and corynebacterium were used as indicator strains in an attempt to isolate corynephages from a large number of soils and from waste-water samples. although no phages capable of producing plaques were isolated, one of the indicator strains used, brevibacterium flavum atcc 14067, was lysogenic for the inducible phage phi ga1. this phage was not observed to form plaques on any of the strains tested. phi ga1 is of b1-morphotype with a linear ... | 1990 | 2391491 |
| microbial metabolism of quinoline and related compounds. vi. degradation of quinaldine by arthrobacter sp. | quinaldine catabolism was investigated with the bacterial strain arthrobacter sp., which is able to grow aerobically in a mineral salt medium with quinaldine as sole source of carbon, nitrogen and energy. the following degradation products of quinaldine were isolated from the culture fluid and identified: 1h-4-oxoquinaldine, n-acetylisatic acid, n-acetylanthranilic acid, anthranilic acid, 3-hydroxy-n-acetylanthranilic acid and catechol. 3-hydroxy-n-acetylanthranilic acid was not further metaboli ... | 1990 | 2076195 |
| trimethyl lead degradation by free and immobilized cells of an arthrobacter sp. and by the wood decay fungus phaeolus schweinitzii. | the continuing production of leaded petrol generates liquid wastes containing recalcitrant trialkyl lead, for which no suitable chemical treatment has been formulated. this investigation explores the feasibility of using microorganisms to catalyse the rate-limiting step of trimethyl lead degradation to dialkyl lead; this disproportionates chemically to give, ultimately, pb2+ which is treatable by classical methods. an arthrobacter sp. and a wood decay macrofungus, phaeolus schweinitzii provide n ... | 1990 | 1366364 |
| action of levan fructotransferase of arthrobacter ureafaciens on three oligosaccharides containing a bifurcose residue. | 1990 | 1368538 | |
| purification and some properties of beta-fructofuranosidase i from arthrobacter sp. k-1. | arthrobacter sp. k-1, isolated from soil, produces beta-fructofuranosidase. this enzyme preparation was separated into three fractions (i, ii, and iii) by butyl-toyopearl 650 m column chromatography. the beta-fructofuranosidase i was purified to homogeneity by disc-electrophoresis after consecutive column chromatographies. the enzyme had a molecular weight of 52,000 by sds-polyacrylamide gel electrophoresis and 51,000 by gel filtration with ultrogel aca 44, and an isoelectric point of 4.3. the e ... | 1990 | 1368550 |
| sites of cadmium uptake in bacteria used for biosorption. | electron microscopy and x-ray spectroscopy were used to determine location and type of cadmium biosorption on and in bacteria, some of which produced extracellular polymers. examined arthrobacter and pseudomonas species appear to have detoxification systems that precipitate cadmium internally irrespective of whether or not they excrete polymers. capsular klebsiella aerogenes strains showed minimal intracellular uptake but over a 5-100 mg dm-3 cd range produced the highest net metal removal level ... | 1990 | 1366533 |
| nadh production from nad+ with a formate dehydrogenase system involving immobilized cells of a methylotrophic arthrobacter strain. | a convenient and economical method of nadh production from nad+ has been established using a formate dehydrogenase system involving immobilized cells of a methanol-utilizing bacterium. arthrobacter sp, km62. four kinds of cell entrapment were studied. an immobilized cell preparation showing a high nadh production activity was obtained by entrapment in a kappa-carrageenan gel lattice. the nadh-producing activity of the immobilized cells was investigated under various conditions. the nadh-producin ... | 1990 | 1366576 |
| transformation of microcrystalline hydrocortisone by free and immobilized cells of arthrobacter simplex. | the transformation of microcrystalline hydrocortisone by free and immobilized cells of arthrobacter simplex resulted in formation of prednisolone. the effect of medium composition, non-ionogenic surfactants and exogenous electron acceptors on the delta 1-dehydrogenase activity of free and immobilized cells is described. | 1990 | 1366456 |
| cell growth and enzyme synthesis of a mutant of arthrobacter sp. (dsm 3747) used for the production of l-amino acids from d,l-5-monosubstituted hydantoins. | a microorganism with the ability to form l-tryptophan from d,l-5-(3-indolyl-methyl)hydantoin (d,l-5-imh) was isolated and identified as arthrobacter sp. (dsm 3747). after isolation of a mutant with high tryptophan production activity but low tryptophan degradation, cultural conditions were optimized to achieve high amounts of biomass with good specific activities concerning the enzymatic hydantoin-cleaving reactions. the ability of the microorganism to perform these bioconversions was found to b ... | 1990 | 1366910 |
| production of l-tryptophan from d,l-5-indolylmethylhydantoin by resting cells of a mutant of arthrobacter species (dsm 3747). | the reaction parameters and the stereospecificity of the enzymatic cleavage of d,l-5-indolylmethylhydantoin in producing l-tryptophan with resting cells of arthrobacter sp. dsm 3747 were studied. when intact cells were tested, the optimal ph was between 8.5 and 9.0 and the optimal temperature was 50 degrees c. both, l-n-carbamoylase and hydantoinase could be stabilized over 24 h at 30 and 40 degrees c by the addition of d,l-5-indolylmethylhydantoin. furthermore, the hydantoinase was stable over ... | 1990 | 1366911 |
| the use of free and immobilised arthrobacter simplex in organic solvent/aqueous two-liquid-phase reactors. | the use of free and immobilised arthrobacter simplex (ncib 8929) for steroid delta 1-dehydrogenation in two-liquid-phase, stirred-tank reactors has been compared. product formation is related to the logarithm of the water-octanol partition coefficient (log p) of the organic solvent employed, but the relationship is different for the two forms of the biocatalyst. no reaction was seen with either biocatalyst in media containing solvents of log p less than or equal to 2.5. for free bacteria, produc ... | 1990 | 1366455 |
| mechanism of gram variability in select bacteria. | gram stains were performed on strains of actinomyces bovis, actinomyces viscosus, arthrobacter globiformis, bacillus brevis, butyrivibrio fibrisolvens, clostridium tetani, clostridium thermosaccharolyticum, corynebacterium parvum, mycobacterium phlei, and propionibacterium acnes, using a modified gram regimen that allowed the staining process to be observed by electron microscopy (j. a. davies, g. k. anderson, t. j. beveridge, and h. c. clark, j. bacteriol. 156:837-845, 1983). furthermore, since ... | 1990 | 1689718 |
| occurrence of protein phosphorylation in various bacterial species. | 1. the occurrence of protein phosphorylation in escherichia coli b, bacillus megaterium, bacillus sphaericus, pseudomonas fluorescens and arthrobacter s1-55, was investigated by means of both in vivo and in vitro experiments. 2. in each bacterial species the presence of several phosphorylated proteins was evidenced by gel electrophoresis and autoradiography after either labelling of growing cells with [32p]orthophosphate or incubating cellular extracts with radioactive atp. 3. the analysis of th ... | 1990 | 1693344 |
| refinement of glucose isomerase from streptomyces albus at 1.65 a with data from an imaging plate. | the structure of 'metal-free' glucose isomerase of streptomyces albus strain number yt atcc 21132 has been analysed and refined at 1.65 a. the space group is i222, with cell dimensions a = 93.9 (1), b = 99.7 (1) and c = 102.9 (1) a, and there is one monomer of the tetrameric molecule per asymmetric unit. the data were recorded from two crystals of the protein using synchrotron radiation from the embl beamline x11 at desy, hamburg. data were recorded with an imaging plate scanner designed and bui ... | 1990 | 2085424 |
| riboflavin-dependent expression of flavoenzymes of the nicotine regulon of arthrobacter oxidans. | in cells of an arthrobacter oxidans riboflavin-dependent mutant the specific activity of the dl-nicotine-inducible nicregulon enzymes nicotine dehydrogenase (ndh, ec 1.5.99.4), 6-hydroxy-l-nicotine oxidase (6-hlno, ec 1.5.3.5) and 6-hydroxy-d-nicotine oxidase (6-hdno, ec 1.5.3.6) was shown to be dependent on the supply of the vitamin in the growth medium. experiments designed to identify at which level riboflavin directs the biosynthesis of these flavoenzymes revealed that the steady-state level ... | 1990 | 1700696 |
| [molecular cloning and expression of a dextranase gene from arthrobacter in streptococcus sanguis]. | the gene coding for a dextranase activity of arthrobacter cb-8, named dex gene, was isolated and cloned into escherichia coli and into streptococcus sanguis. the gene library was screened by transparent halo formation around the colonies grown on agar medium containing blue dextran. dna fragment consisting of about 3,200 base pairs was prepared for further cloning procedures. dextranase activity was detected in the periplasmic space of e. coli clones, using puc19, pva 838 and their derivatives. ... | 1990 | 2135413 |
| sa11 rotavirus is specifically inhibited by an acetylated sialic acid. | bovine salivary mucin (bsm) inhibits rotavirus replication in vitro and in vivo. the inhibitory effect of bsm in vitro is abolished by arthrobacter ureafaciens neuraminidase but not by clostridia perfringens neuraminidase; it is abolished by mild base deacetylation but not by influenza c acetylesterase. the data suggest that sa11 rotavirus binds to a specific sialic acid structure on bsm different from the sialic acids recognized by other viruses. | 1990 | 2153181 |
| reductive trapping of substrate to methylamine oxidase from arthrobacter p1. | methylamine oxidase (ec 1.4.3.6) from arthrobacter p1 was inactivated by nacnbh3 in the presence of [14c]benzylamine, leading to the incorporation of 1 mol of radiolabeled substrate/mol of enzyme subunit at complete inactivation. by contrast, no labeling of enzyme was observed using [3h]nacnbh3 as reductant. these results are analogous to those previously reported for the eukaryotic enzyme, bovine serum plasma amine oxidase [(1987) j. biol. chem. 262, 962-965]. the observed pattern of labeling i ... | 1990 | 2155832 |
| catalytic mechanism of xylose (glucose) isomerase from clostridium thermosulfurogenes. characterization of the structural gene and function of active site histidine. | the gene coding for thermophilic xylose (glucose) isomerase of clostridium thermosulfurogenes was isolated and its complete nucleotide sequence was determined. the structural gene (xyla) for xylose isomerase encodes a polypeptide of 439 amino acids with an estimated molecular weight of 50,474. the deduced amino acid sequence of thermophilic c. thermosulfurogenes xylose isomerase displayed higher homology with those of thermolabile xylose isomerases from bacillus subtilis (70%) and escherichia co ... | 1990 | 2229064 |
| mechanism of enzymatic dehalogenation of pentachlorophenol by arthrobacter sp. strain atcc 33790. | pentachlorophenol (pcp) dehalogenase from arthrobacter sp. strain atcc 33790 converts pcp to tetrachlorohydroquinone. in labeling experiments with h(2)18o or 18o2, only with h(2)18o was labeled product found. however, unlabeled tetrachlorohydroquinone became labeled after incubation with the enzyme in h(2)18o. therefore, distinction between an oxygenolytic or a hydrolytic dehalogenation mechanism for the pcp dehalogenase is not possible. | 1990 | 2254286 |
| growth of group a rotaviruses in a human liver cell line. | recent observations in children with rotavirus gastroenteritis and in infant mice given rotavirus vaccine by oral administration suggest that this well-known gastrointestinal pathogen may infect the liver. to examine this possibility, the susceptibility of hep g2 cells to infection with a variety of rotavirus strains was tested. these cells were used because they are considered to be well differentiated and exhibit many liver-specific functions. the hep g2 cells supported the growth of the simia ... | 1990 | 2170264 |
| acii, a unique restriction endonuclease from arthrobacter citreus which recognizes 5' ccgc 3'. | 1990 | 2170952 | |
| methylamine oxidase from arthrobacter p1. | 1990 | 2280707 | |
| a repeated decapeptide motif in the c-terminal domain of the ribosomal rna methyltransferase from the erythromycin producer saccharopolyspora erythraea. | re-analysis of the primary structure of the ribosomal rna n-methyltransferase that confers self-resistance on the erythromycin-producing bacterium saccharopolyspora erythraea has confirmed the presence of a c-terminal domain containing extensive repeat sequences. nine tandem repeats can be discerned, with a decapeptide consensus sequence ggrx(h/r)gdrrt, although no single residue is wholly invariant. this highly polar, potentially flexible domain, which is predicted to adopt either a random coil ... | 1990 | 2335200 |
| a survey of the microflora of raw and pasteurized milk and the sources of contamination in a milk processing plant in addis ababa, ethiopia. | the microorganisms present in raw and pasteurized milk and the sources of contamination in the milk after it had arrived at the processing plant in addis ababa were studied. the lowest count registered for raw milk samples was 4 x 10(7) cfu/ml while the highest was 1 x 10(9) cfu/ml. pasteurized milk had mesophilic aerobic counts of 7 x 10(5) cfu/ml as it left the pasteurizing unit, but the population increased 2- to 4-fold as a result of subsequent contamination. of the total counts in raw milk, ... | 1990 | 2345191 |
| effect of organic amendments on bacterial multiplication in lake water. | in cayuga lake water amended with 30 micrograms of glucose or amino acids per ml, an added strain of pseudomonas fluorescens and indigenous bacteria grew extensively, pseudomonas sp. b4 and two rhizobia multiplied at a moderate extent, and introduced escherichia coli and klebsiella pneumoniae multiplied but to only a slight degree. the amendments did not enhance growth of micrococcus flavus and arthrobacter citreus, and an asporogenous strain of bacillus subtilis decreased in numbers. the pseudo ... | 1990 | 2372214 |
| functional analysis of the 5' regulatory region and the uug translation initiation codon of the arthrobacter oxidans 6-hydroxy-d-nicotine oxidase gene. | a functional analysis of the arthrobacter oxidans 6-hydroxy-d-nicotine oxidase (6-hdno) gene promoter (-35 region ttgaca and -10 region tatcaat) and the uug translation start codon was performed using site-directed mutagenesis. deletion of the c residue from the -10 promoter region or mutations introduced upstream of the -10 region resulted in an increased 6-hdno expression in escherichia coli cells in vivo and in both e. coli and a. oxidans coupled transcription-translation systems in vitro. fr ... | 1990 | 2381422 |
| occurrence and ultrastructural characterization of bacteria in association with and isolated from azolla caroliniana. | the occurrence and ultrastructure of bacteria in leaf cavities of symbiotic azolla caroliniana were examined by transmission electron microscopy. bacteria were observed in all leaf cavities of azolla cultures. five ultrastructurally distinct types of bacteria were observed in each individual leaf cavity. features used to characterize the bacteria included morphology, cell wall structure, and cytoplasmic organization. at least one gram-positive and as many as four gram-negative types of bacteria ... | 1991 | 1785935 |
| transglycosylation activity of endo-beta-n-acetylglucosaminidase from arthrobacter protophormiae. | endo-beta-n-acetylglucosaminidase from arthrobacter protophormiae has transglycosylation activity. treatment of (man)6(glcnac)2asn with the enzyme in the presence of free n-acetylglucosamine gave a mixture of (man)6glcnac and (man)6glcnac beta 1----4glcnac mixture. n-acetylglucosamine at the reducing end of the latter sugar chain was found by hplc of the carbohydrate composition and of an exoglycosidase digest of the pyridylamino derivative of the reducing-end residue, and by 400 mhz 1h-nmr spec ... | 1991 | 1776953 |
| 3,4-dihydroxyxanthone dioxygenase from arthrobacter sp. strain gfb100. | bacterial extradiol ring-fission dioxygenases play a critical role in the transformation of multiring aromatic compounds to more readily biodegradable aromatic or aliphatic intermediates. arthrobacter sp. strain gfb100 utilizes an extradiol meta-fission dioxygenase, 3,4-dihydroxyxanthone dioxygenase (dhxd), in the catabolism of the three-ring oxygen heterocyclic compound xanthone. in this paper, we show that dhxd is a cytosolic enzyme, induced by growth on xanthone and maximally expressed during ... | 1991 | 1768091 |
| [quantitative description of microbial growth in a batch culture depending on the physiologic state of inocula]. | the dynamics of biomass production and the respiration rate of five microorganisms grown as batch cultures were studied in detail. cell suspensions with a known physiological state, i.e. chemostat cultures grown at a particular d value, as well as quasi steady-state populations cultivated with slow feeding and long energy-source starvation were used as inocula. the microorganisms were arbitrary subdivided into two groups. the biomass of pseudomonas fluorescens, bacillus subtilis and debaryomyces ... | 1991 | 1770867 |
| the breast tumor-associated epitope defined by monoclonal antibody 3e1.2 is an o-linked mucin carbohydrate containing n-glycolylneuraminic acid. | the breast cancer-associated epitope (mammary serum antigen or msa) defined by monoclonal antibody (mab) 3e1.2 is a neuraminidase-sensitive carbohydrate expressed on muc-1-encoded molecules. however, the reactivity of mab 3e1.2 is also reduced by protease treatment of the mucin, which suggests that 3e1.2 binds to multimers of the sialylated carbohydrate in a protein conformation-dependent manner. the common n-acetyl derivative of neuraminic acid (5-acetylneuraminic acid) is not involved in the e ... | 1991 | 1718585 |
| substrate interactions of benzene, toluene, and para-xylene during microbial degradation by pure cultures and mixed culture aquifer slurries. | benzene, toluene, and p-xylene (btx) were degraded by indigenous mixed cultures in sandy aquifer material and by two pure cultures isolated from the same site. although btx compounds have a similar chemical structure, the fate of individual btx compounds differed when the compounds were fed to each pure culture and mixed culture aquifer slurries. the identification of substrate interactions aided the understanding of this behavior. beneficial substrate interactions included enhanced degradation ... | 1991 | 1746958 |
| copper in biological systems. a report from the 6th manziana conference, september 23-27, 1990. | enzymes and proteins: ao, amine oxidase; and as proposed in reference 3, bsao, bovine serum ao; ssao, swine serum ao; skdao, swine kidney ao; psao, pea seedling ao; apao, arthrobacter p1ao; madh, methylamine dehydrogenase; aao, ascorbic acid oxidase; alpha-ae, alpha-amidating enzyme; az, azurin; cox, cytochrome c oxidase; cp, ceruloplasmin; dbh, dopamine beta-hydroxylase; go, galactose oxidase; hc, hemocyanin; mt, metallotheonein; nir, nitrite reductase; sod, superoxide dismutase. cofactors: dop ... | 1991 | 1757786 |
| phylogeny of the whipple's-disease-associated bacterium. | efforts to culture and identify the intracellular bacteria associated with whipple's disease have been unsuccessful. nucleotide sequencing and amplification by the polymerase chain reaction was done on the bacterial 16 s ribosomal dna present in a small-bowel biopsy specimen taken from a patient with whipple's disease. a search by computer for similar rrna sequences filed in databases showed the whipple's-associated organism to be most similar to bacteria of the rhodococcus, streptomyces, and ar ... | 1991 | 1714530 |
| structural determination of d-mannans of pathogenic yeasts candida stellatoidea type i strains: timm 0310 and atcc 11006 compared to ifo 1397. | the structures of the cell-wall d-mannans of pathogenic yeasts of candida stellatoidea type i strains, ifo 1397, timm 0310, and atcc 11006, were investigated by mild acid and, alkaline hydrolysis, by digestion with the arthrobacter gjm-1 strain exo-alpha-d-mannosidase, and by acetolysis. the modified d-mannans and their degradation products were studied by 1h- and 13c-n.m.r. analyses. d-manno-oligosaccharides released by acid treatment from the parent d-mannans were identified as the homologous ... | 1991 | 1954627 |
| the combination of a bacterial polysaccharide and tamoxifen inhibits angiogenesis and tumour growth. | 1991 | 1713945 | |
| choline oxidase, a catabolic enzyme in arthrobacter pascens, facilitates adaptation to osmotic stress in escherichia coli. | choline oxidase (ec 1.1.3.17) is a bifunctional enzyme that is capable of catalyzing glycine betaine biosynthesis from choline via betaine aldehyde. a gene (cox) encoding this enzyme in the gram-positive soil bacterium arthrobacter pascens was isolated and characterized. this gene is contained within a 1.9-kb fragment that encodes a polypeptide of approximately 66 kda. transfer of this gene to an escherichia coli mutant that is defective in betaine biosynthesis resulted in an osmotolerant phenot ... | 1991 | 1987142 |
| direct evidence that ganglioside is an integral component of the thyrotropin receptor. | gangliosides were extracted from purified human and porcine thyrotropin (tsh) receptors (tsh-r) and were detected by probing with an 125i-labeled sialic acid-specific lectin, limax flavus agglutinin. gangliosides copurified with human and porcine tsh-r migrated between monosialoganglioside gm1 and disialoganglioside gd1a. ceramide glycanase digestion of the purified human tsh-r-associated glycolipid confirmed its ganglioside nature. it was resistant to vibrio cholerae sialidase, which digests al ... | 1991 | 2000404 |
| switching substrate preference of thermophilic xylose isomerase from d-xylose to d-glucose by redesigning the substrate binding pocket. | the substrate specificity of thermophilic xylose isomerase from clostridium thermosulfurogenes was examined by using predictions from the known crystal structure of the arthrobacter enzyme and site-directed mutagenesis of the thermophile xyla gene. the orientation of glucose as a substrate in the active site of the thermophilic enzyme was modeled to position the c-6 end of hexose toward his-101 in the substrate-binding pocket. the locations of met-87, thr-89, val-134, and glu-180, which contact ... | 1991 | 2023950 |
| metabolism of carbamate insecticides by resting cells and cell-free preparations of a soil bacterium, arthrobacter sp. | 1991 | 2032002 | |
| occurrence of two structural types of mercury reductases among gram-positive bacteria. | structural variants of mercury reductase containing the n-terminal domain, which is easily cleaved by trypsin, have been found in gram-positive bacteria with a low genomic g + c content (bacillus, staphylococcus and, possibly, some other genera). mercury reductases without the n-terminal domain and relatively resistant to limited proteolysis are typical for gram-positive bacteria with a high genomic g + c content (arthrobacter, citreobacterium, micrococcus, mycobacterium, rhodococcus). both type ... | 1991 | 2040434 |
| stereospecific microbial reduction of 4,5-dihydro-4-(4-methoxyphenyl)-6-(trifluoromethyl-1h-1)-benzazepin+ ++-2-o ne. | a key intermediate, (3r-cis)-1,3,4,5-tetrahydro-3-hydroxy-4-(4-methoxyphenyl)-6-(trifluorome thyl)- 2h-1-benzazepin-2-one (compound ii or sq32191), with high optical purity was made by the stereoselective microbial reduction of the parent ketone 1. several strains of bacterial and yeast cultures were screened for the ability to catalyse the stereoselective reduction of 4,5-dihydro-4-(4-methoxyphenyl)-6-(trifluoromethyl)-1h-1-benzazepin++ +-2,3-dione [compound i or sq32425]. microorganisms from t ... | 1991 | 1368001 |
| [cloning of the arthrobacter globiformis fcba gene for dehalogenase and construction of a hybrid pathway of 4-chlorobenzoic acid degradation in pseudomonas putida]. | the artrobacter globiformis kzt1 fcba gene responsible for dehalogenase (4-chlorobenzoate-4-hydroxylase) activity was cloned in escherichia coli and pseudomonas putida cells. the character of the fcba gene expression was studied. notwithstanding amplification of the gene dose and control of the inducible plac promoter, the level of substrate dehalogenation by recombinant e. coli strains was lower, as compared with that in the original kzt1 strain. cloning of the fcba gene in p. putida kz6r cells ... | 1991 | 1879677 |
| cloning and expression of the arthrobacter globiformis kzt1 fcba gene encoding dehalogenase (4-chlorobenzoate-4-hydroxylase) in escherichia coli. | the fsba gene controlling the first step of 4-chlorobenzoic acid (4cba) metabolism in the gram-positive soil bacterium arthrobacter globiformis kzt1 has been cloned and analysed in escherichia coli. the e. coli minicells analysis showed that a polypeptide(s) with mr = 58 kda (and/or mr = 32 kda) can be the fcba product(s). despite the gene dose amplification and control of the e. coli inducible plac promoter, the level of functional expression of the fcba gene in e. coli cells seems comparable o ... | 1991 | 1884992 |
| genetic control of degradation of chlorinated benzoic acids in arthrobacter globiformis, corynebacterium sepedonicum and pseudomonas cepacia strains. | the strains of arthrobacter globiformis kzt1, corynebacterium sepedonicum kz4 and pseudomonas cepacia kz2 capable of early dehalogenation and complete oxidation of 4-chloro-, 2,4-dichloro-and 2-chlorobenzoic acids, respectively, have been analyzed for the origin of the genetic control of degradation. the occurrence and molecular sizes of plasmids in all the strains have been established. plasmid pbs1501 was shown to control 4-chlorobenzoate dehalogenation in the case of kzt1 strain. the same pos ... | 1991 | 1884993 |
| immobilization of arthrobacter simplex in thermally reversible hydrogels: effect of gel hydrophobicity on steroid conversion. | arthrobacter simplex cells have been immobilized in a series of thermally reversible hydrogels having different gel hydrophobicities. steroid conversion from hydrocortisone to prednisolone via the delta 1-dehydrogenase system was greatly affected by the relative hydrophobicities of the gel matrices, which were prepared by copolymerizing varying ratios of n-isopropylacrylamide to acrylamide. the characteristics of the immobilized cells, such as optimal temperatures, km values, and the effects of ... | 1991 | 1367990 |
| continuous delta 1-hydrocortisone dehydrogenation with in situ product recovery. | a continuous aerated process for delta 1-hydrocortisone dehydrogenation by polyacrylamide-hydrazide (paah) bead-entrapped a. simplex cells was developed. the process allows for stable conversion of 1.6 g l-1 hydrocortisone (x 5 the solubility in water), made possible by the incorporation of selected cosolvent [5% (v/v) triethyleneglycol]. a large difference in substrate and product solubilities in the cosolvent-buffer medium allowed for in situ product recovery in an aerated, fluidized-bed, immo ... | 1991 | 1367997 |
| delta'-dehydrogenation of steroids by arthrobacter simplex immobilized in calcium polygalacturonate beads. | arthrobacter simplex atcc 6946 (viable cells) was immobilized in a calcium polygalacturonate gel. the trapped cells were used for repeated batchwise bioconversion of steroids. reichstein's compound s and hydrocortisone were dehydrogenated introducing a double bond between c1 and c2 of ring a. the products 1-dehydro s and prednisolone, respectively, were identified by high pressure liquid chromatography. steroid dehydrogenase activity increased in the system when an artificial electron acceptor, ... | 1991 | 1367800 |
| structural organization of the corynebacterium glutamicum plasmid pcg100. | pcg100, a 3 kb cryptic plasmid of corynebacterium glutamicum atcc 13058, probably identical with psr1 from c. glutamicum atcc 19223, was characterized. the minimum region for autonomous replication was shown to be contained on a 1.9 kb bglii-ncoi fragment; a 380 bp hindiii-sphi fragment can replicate in the presence of the parental plasmid, which presumably provides a trans-acting replication factor. derivatives of pcg100 are able to replicate in several corynebacterium, brevibacterium and arthr ... | 1991 | 1748866 |
| l-lysine production by s-2-aminoethyl-l-cysteine-resistant mutants of arthrobacter globiformis. | using mutagenesis with n-methyl-n'-nitro-n-nitrosoguanidine, a number of homoserine auxotrophs have been isolated from a glutamate-producing arthrobacter globiformis excreting l-lysine in good amounts. for further improvement, mutants resistant to the lysine analog s-(2-aminoethyl)-l-cysteine have been isolated from homoserine auxotrophs. for the three potent mutants tested, white's medium was found to be the best. glucose, ammonium nitrate and biotin were found to be optimum at 280 mmol/l, 40 m ... | 1991 | 1841850 |
| isolation method for lysine-excreting mutants of arthrobacter globiformis. | to improve the yield of lysine by the isolate, auxotrophic mutants were isolated. among the mutants, only one auxotrophic mutant required vitamin b12. this mutant produced alpha-alanine. about 200 mutants resistant to the lysine analog s-(2-aminoethyl)-l-cysteine were isolated and some of them produced well above the wild type. | 1991 | 1841851 |
| l-phenylalanine production by double auxotrophic mutants of arthrobacter globiformis. | a number of tryptophan-plus-tyrosine double auxotrophs have been isolated from a glutamate producing arthrobacter globiformis excreting l-phenylalanine by two-step mutagenesis with n-methyl-n'-nitro-n-nitrosoguanidine. for the three potent mutants tested the medium of alföldi was found to be the best. the optimum tryptophan, tyrosine and biotin concentrations for phenylalanine production of these mutants were 0.5 mmol/l, 0.1 mmol/l and 5 micrograms/l, respectively. at these levels strain tt-39 y ... | 1991 | 1841857 |
| purification and characterization of haloalcohol dehalogenase from arthrobacter sp. strain ad2. | an enzyme capable of dehalogenating vicinal haloalcohols to their corresponding epoxides was purified from the 3-chloro-1,2-propanediol-utilizing bacterium arthrobacter sp. strain ad2. the inducible haloalcohol dehalogenase converted 1,3-dichloro-2-propanol, 3-chloro-1,2-propanediol, 1-chloro-2-propanol, and their brominated analogs, 2-bromoethanol, as well as chloroacetone and 1,3-dichloroacetone. the enzyme possessed no activity for epichlorohydrin (3-chloro-1,2-epoxypropane) or 2,3-dichloro-1 ... | 1991 | 1846134 |
| d-xylose (d-glucose) isomerase from arthrobacter strain n.r.r.l. b3728. gene cloning, sequence and expression. | arthrobacter strain n.r.r.l. b3728 superproduces a d-xylose isomerase that is also a useful industrial d-glucose isomerase. the gene (xyla) that encodes it has been cloned by complementing a xyla mutant of the ancestral strain, with the use of a shuttle vector. the 5' region shows strong sequence similarity to escherichia coli consensus promoters and ribosome-binding sequences and allows high levels of expression in e. coli. the coding sequence shows similarity to those for other d-xylose isomer ... | 1991 | 1854339 |
| molecular cloning and nucleotide sequencing of the arthrobacter dextranase gene and its expression in escherichia coli and streptococcus sanguis. | a bacterial strain, which assimilated dextran and water-insoluble glucan produced by streptococcus mutans, was isolated from soil. the bacterium produced and secreted potent dextranase activity, which was identified as arthrobacter sp. and named cb-8. the dextranase was purified and some enzymatic properties were characterized. the enzyme efficiently decomposed the water-insoluble glucan as well as dextran. a gene library from the bacteria was constructed with escherichia coli, using plasmid puc ... | 1991 | 1859672 |
| biodegradation of refractory hydrocarbon biomarkers from petroleum under laboratory conditions. | biomarkers are of great value in petroleum exploration because they provide essential information about the geological history of oils and source rocks. steranes are of particular importance as they can be related to naturally occurring precursors. these compounds generally experience intense biodegradation, however, which alters their original distribution and obscures the information that they carry regarding oil maturity and source material. in an attempt to identify the microorganisms respon ... | 1991 | 2052089 |
| evidence for two distinct phosphonate-degrading enzymes (c-p lyases) in arthrobacter sp. glp-1. | arthrobacter sp. glp-1 can utilize a wide range of organophosphonates as its sole source of phosphorus. the in-situ formation of sarcosine and methane from glyphosate and methanephosphonic acid respectively was studied. these two processes are differentially induced during phosphorus-deprivation. methanephosphonic acid strongly inhibits glyphosate degradation (i50 10 microm), but glyphosate has very little effect on methane generation (i50 150 mm). the pattern of inhibition by other organophosph ... | 1991 | 1368477 |
| the organic functional group in copper-containing amine oxidases. resonance raman spectra are consistent with the presence of topa quinone (6-hydroxydopa quinone) in the active site. | resonance raman spectroscopy has been used to probe the structure of the organic cofactor in copper-containing amine oxidases from bovine plasma, porcine kidney, pea seedlings, and the bacterium arthrobacter p1. the enzymes were first derivatized with phenylhydrazine or p-nitrophenylhydrazine; resonance raman spectra were obtained on the intact derivatized enzymes and on a derivatized active-site peptide isolated from bovine plasma amine oxidase. spectra of the intact amine oxidase phenylhydrazo ... | 1991 | 1900285 |
| mannopine and mannopinic acid as substrates for arthrobacter sp. strain mba209 and pseudomonas putida na513. | the characteristics of mannopine and mannopinic acid utilization by agrobacterium tumefaciens b6s3, arthrobacter sp. strain mba209, and pseudomonas putida na513 were studied. strain b6s3 utilized the four mannityl opines, mannopine, mannopinic acid, agropine, and agropinic acid. it also utilized several mannityl opine analogs, which were modified in either the sugar or the amino acid moiety. it utilized mannopine more rapidly after preincubation on mannopine, mannopinic acid, or glutamine than a ... | 1991 | 1902209 |
| enzymatic synthesis of novel oligosaccharides by use of transglycosylation activity of endo-beta-n-acetylglucosaminidase from arthrobacter protophormiae. | the transglycosylation activity of endo-beta-n-acetylglucosaminidase from arthrobacter protophormiae was used for the enzymatic synthesis of novel oligosaccharides. when (man)6(glcnac)2asn was used as a substrate for the transglycosylation, (man)6glcnac-glc, (man)6glcnac-man, (man)6glcnac-chitobiose, and (man)6glcnac-gentiobiose were synthesized. their structures were identified by hplc, ion spray mass spectrometry, and digestion with glycosidases. endo-beta-n-acetylglucosaminidases hydrolyzed t ... | 1991 | 1804102 |
| determination of glycosylation sites using a protein sequencer and deglycosylation of native yeast invertase by endo-beta-n-acetylglucosaminidase. | endo-beta-n-acetylglucosaminidase from arthrobacter protophormiae was tested for its capacity to release n-linked sugar chains from native yeast invertase. the enzyme liberated about 80% of the sugar chains from the native invertase. deglycosylated invertase was digested by chymotrypsin or pepsin, and twelve n-acetylglucosamine-containing glycopeptides were isolated. the amino acid sequences of these glycopeptides were analyzed by a protein sequencer, and the elution position of 4-l-aspartylglyc ... | 1991 | 1805802 |
| a cu(i)-semiquinone state in substrate-reduced amine oxidases. | the role of copper in copper-containing amine oxidases has long been a source of debate and uncertainty. numerous electron paramagnetic resonance (epr) experiments, including rapid freeze-quench studies, have failed to detect changes in the copper oxidation state in the presence of substrate amines. one suggestion that copper reduction might occur, has never been confirmed. copper amine oxidases contain another cofactor, recently identified as 6-hydroxydopa quinone (topa quinone), which is reduc ... | 1991 | 1846226 |
| d-xylose (d-glucose) isomerase from arthrobacter strain n.r.r.l. b3728. purification and properties. | d-xylose (d-glucose) isomerase was purified to homogeneity in yields of approx. 1 g/kg of wet cells from a strain of arthrobacter that produces it as about 10% of total soluble protein. it is a tetramer of identical 43,114 da subunits containing a preponderance of acidic residues and no cysteine. partial protein sequences were determined as a step to gene cloning. it requires mg2+, co2+ or mn2+ for activity, mg2+ being best; ca2+ is an inhibitor, competitive with mg2+. it is a good d-glucose iso ... | 1991 | 1854338 |
| marine biosurfactants, ii. production and characterization of an anionic trehalose tetraester from the marine bacterium arthrobacter sp. ek 1. | within a screening for biosurfactants we could isolate various n-alkanes utilizing marine bacteria which were capable of synthesizing glycolipids. one strain was identified as arthrobacter sp. ek 1 which produced trehalose lipids. after purification by column and thick layer chromatography the main fraction, an anionic 2,3,4,2'-trehalose tetraester, was obtained. the chain lengths of fatty acids ranged from 8 up to 14, furthermore succinate could be detected. since the place of substitution of s ... | 1991 | 1878107 |
| marine biosurfactants, i. screening for biosurfactants among crude oil degrading marine microorganisms from the north sea. | three bacterial strains of marine origin were isolated during a screening for biosurfactants among n-alkane degrading microorganisms. one strain-identified as alcaligenes sp. mm1-produced a novel glucose lipid. in the case of arthrobacter sp. ek 1 the well-known trehalose tetraester was found as major component. from another pure culture classified as arthrobacter sp. si 1, extracellular emulsifying agents with properties indicating high molecular weight substances were detected. furthermore tre ... | 1991 | 1878106 |
| antagonistic effect of coryneform bacteria from red smear cheese against listeria species. | a total of 187 coryneform bacteria were isolated from red smear and screened for inhibitory effects against 16 strains of listeria species. culture filtrates from brevibacterium linens (16 strains), arthrobacter nicotianae (4 strains) and arthrobacter nucleogenes (3 strains) showed clear zones of inhibition. the antagonistic effect was seen against 26 to 87% of 91 listeria strains tested. a. nicotianae and a. nucleogenes were more effective against listeria innocua and listeria ivanovii than aga ... | 1991 | 1909545 |
| experimental apparatus for selection of adherent microorganisms under stringent growth conditions. | a bioreactor apparatus is described for studying bacterial attachment. a cyclic, on-off, flow regime was imposed within the apparatus. model calculations illustrate the utility of this flow pattern in the selection and maintenance of slow-growing, adherent organisms. the apparatus is believed to have general utility in testing bacterial attachment influenced by many types of experimental or environmental constraints, including variations in fluid dynamics, presence of toxic substances (metals or ... | 1991 | 1892388 |
| description of the erythromycin-producing bacterium arthrobacter sp. strain nrrl b-3381 as aeromicrobium erythreum gen. nov., sp. nov. | arthrobacter sp. strain nrrl b-3381t (t = type strain) is a nonmycelial, nonsporulating actinomycete that produces the macrolide antibiotic erythromycin. this bacterium differs in many ways from the type species of the genus arthrobacter (arthrobacter globiformis), suggesting that a taxonomic revision is appropriate. the g + c content of strain nrrl b-3381t dna is 71 to 73 mol%, and the peptidoglycan of this organism contains ll-diaminopimelic acid. evolutionary distance data obtained from 16s r ... | 1991 | 1883712 |
| biodegradation by an arthrobacter species of hydrocarbons partitioned into an organic solvent. | an arthrobacter strain mineralized naphthalene and n-hexadecane dissolved in 2,2,4,4,6,8,8-heptamethylnonane. the extent of mineralization increased with greater volumes of solvent. measurements under aseptic conditions of the partitioning of naphthalene into the aqueous phase from the solid phase or from heptamethylnonane showed that the rates were rapid and did not limit mineralization. the rate of mineralization of hexadecane was rapid, although partitioning of the compound into aqueous solut ... | 1991 | 16348485 |