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[effect of elevation of 1-hydroxy-2-naphthoic acid concentration on the lag phase of arthrobacter sp. k3: kinetic analysis]. 200919449734
viability and potential for immigration of airborne bacteria from africa that reach high mountain lakes in europe.we have analysed the diversity of the bacteria, which grow after addition of concentrated airborne particles and desert dust in different microcosms combinations with water samples from oligotrophic alpine lakes. we used, on the one hand, airborne bacteria transported by an african dust plume and collected in a high mountain area in the central pyrenees (spain). on the other hand, we collected desert dust in mauritania (c. 3000 km distance, and a few days estimated airborne journey), a known sou ...200919453609
microbial responses to mustard gas dumped in the baltic sea.microbiological studies were carried out on chemical weapon dump sites in the baltic sea. the effect of mustard gas hydrolysis products (mghps) on marine microbiota and the ability of microorganisms to degrade mghps were studied. many stations at the dump sites demonstrated reduced microbial diversity, and increased growth of species able to use mustard gas hydrolysis products as sole source of carbon. significant amounts of mghp-degrading bacteria were revealed in the near-bottom water. the mgh ...200919481794
[effect of a novel fibrinolytic enzyme fa-i on thrombosis and thrombolysis].to study the effect of fa-i, a novel fibrinolytic enzyme isolated from the metabolite of arthrobacter sp. dr-536, on thrombosis and thrombolysis in vivo.200919462909
bacterial diversity of soil samples from the western himalayas, india.high-altitude cold habitats of the himalayas are little explored with respect to bacterial diversity. diverse bacterial species and phylotypes obtained by culture-dependent and culture-independent approaches are reported here. phylogenetic analysis and modulation of bacterial diversity with altitude and available organic carbon content are also described. psychrophilic and psychrotolerant bacteria dominated the himalayan habitats, accounting for 60% of the cultivated strains. isolates produced o ...200919483785
molecular design of a nylon-6 byproduct-degrading enzyme from a carboxylesterase with a beta-lactamase fold.a carboxylesterase with a beta-lactamase fold from arthrobacter possesses a low level of hydrolytic activity (0.023 mumol.min(-1).mg(-1)) when acting on a 6-aminohexanoate linear dimer byproduct of the nylon-6 industry (ald). g181d/h266n/d370y triple mutations in the parental esterase increased the ald-hydrolytic activity 160-fold. kinetic studies showed that the triple mutant possesses higher affinity for the substrate ald (k(m) = 2.0 mm) than the wild-type ald hydrolase from arthrobacter (k(m) ...200919476493
microbial ecology of gorgonzola rinds and occurrence of different biotypes of listeria monocytogenes.in this study we investigated the microbiota of gorgonzola rinds and maturing shelf swabs collected in 5 different maturing cellars in the northwest part of italy, in association with the detection and characterization of listeria monocytogenes. culture-dependent and -independent methods were performed in order to profile the main microbial populations present on the rinds and in the maturing shelves and species-specific pcr and pulsed field gel electrophoresis (pfge) were used to identify and t ...200919477544
batch and continuous biosorption of cu(2+) by immobilized biomass of arthrobacter sp.the ability of free and polysulphone immobilized biomass of arthrobacter sp. to remove cu(2+) ions from aqueous solution was studied in batch and continuous systems. the langmuir and freundlich isotherm models were applied to the data. the langmuir isotherm model was found to fit the sorption data indicating that sorption was monolayer and uptake capacity (q(o)) was 175.87 and 158.7mg/g for free and immobilized biomass respectively at ph 5.0 and 30 degrees c temperature, which was also confirmed ...200919487070
identification of a novel self-sufficient styrene monooxygenase from rhodococcus opacus 1cp.sequence analysis of a 9-kb genomic fragment of the actinobacterium rhodococcus opacus 1cp led to identification of an open reading frame encoding a novel fusion protein, stya2b, with a putative function in styrene metabolism via styrene oxide and phenylacetic acid. gene cluster analysis indicated that the highly related fusion proteins of nocardia farcinica ifm10152 and arthrobacter aurescens tc1 are involved in a similar physiological process. whereas 413 amino acids of the n terminus of stya2 ...200919482928
glycinebetaine-induced water-stress tolerance in coda-expressing transgenic indica rice is associated with up-regulation of several stress responsive genes.rice (oryza sativa l.), a non-accumulator of glycinebetaine (gb), is highly susceptible to abiotic stress. transgenic rice with chloroplast-targeted choline oxidase encoded by the coda gene from arthrobacter globiformis has been evaluated for inheritance of transgene up to r5 generation and water-stress tolerance. during seedling, vegetative and reproductive stages, transgenic plants could maintain higher activity of photosystem ii and they show better physiological performance, for example, enh ...200919490479
optimization of physical parameters for lipase production from arthrobacter sp. bgcc#490.the physical parameters for the production of thermostable, alkaline lipase from arthrobacter sp. bgcc# 490 were optimized using response surface methodology (rsm), employing face centered central composite design (fcccd). the design was employed by selecting ph, temperature and incubation period as the model factors and to achieve maximum yield, interaction of these factors was studied by rsm. a second-order quadratic model and response surface method showed that the optimum conditions for lipa ...200919517996
azolla-anabaena's behaviour in urban wastewater and artificial media--influence of combined nitrogen.the results of using the nitrogen fixing symbiotic system azolla-anabaena to improve the quality of treated urban wastewater, particularly on what concerns phosphorus removal efficiencies (40-65%), obtained in continuous assays performed during the past few years and presented earlier, were very promising. nevertheless, the presence of combined nitrogen in some wastewaters can compromise the treatment efficiency. the main goal of this work was to compare plants behaviour in wastewater and in min ...200919559459
two alternative modes for optimizing nylon-6 byproduct hydrolytic activity from a carboxylesterase with a beta-lactamase fold: x-ray crystallographic analysis of directly evolved 6-aminohexanoate-dimer hydrolase.promiscuous 6-aminohexanoate-linear dimer (ald)-hydrolytic activity originally obtained in a carboxylesterase with a beta-lactamase fold was enhanced about 80-fold by directed evolution using error-prone pcr and dna shuffling. kinetic studies of the mutant enzyme (hyb-s4m94) demonstrated that the enzyme had acquired an increased affinity (k(m) = 15 mm) and turnover (k(cat) = 3.1 s(-1)) for ald, and that a catalytic center suitable for nylon-6 byproduct hydrolysis had been generated. construction ...200919521995
synthesis of 9-beta-d-arabinofuranosylguanine by combined use of two whole cell biocatalysts.unlike the preparation of other purine nucleosides, transglycosylation from a pyrimidine nucleoside and guanine is difficult because of the low solubility of this base. thus, another strategy, based on the coupled action of two whole cell biocatalyzed reactions, transglycosylation and deamination, was used. enterobacter gergoviae and arthrobacter oxydans were employed to synthesize 9-beta-d-arabinofuranosylguanine (arag), an efficient anti leukemic drug.200919523826
isolation and characterization of arthrobacter sp. hy2 capable of degrading a high concentration of p-nitrophenol.a soil bacterium strain, capable of using p-nitrophenol (pnp) as its sole source of carbon and energy, was isolated by enrichment on minimal salt medium (msm). on the basis of a phylogenetic analysis of 16s rrna gene sequences the bacterium is a species of arthrobacter, closely related to arthrobacter ureafaciens dsm 20126. this strain has an unusually high substrate tolerance for pnp degradation in msm. greatest degradation of pnp was observed at 30 degrees c and under slightly alkaline ph (ph ...200919540107
production of acrylic acid from acrylonitrile by immobilization of arthrobacter nitroguajacolicus zjutb06-99.immobilized cells of arthrobacter nitroguajacolicus zjutb06-99 capable of producing nitrilase was used for biotransformation of acrylonitrile to acrylic acid. six different entrapment matrixes were chosen to search for a suitable support in terms of nitrilase activity. ca-alginate proved to be more advantageous over other counterparts in improvement of the biocatalyst activity and bead mechanical strength. the effects of sodium alginate concentration, cacl2 concentration, bead diameter, and rati ...200919597316
selection of a cold-adapted bacterium for bioremediation of wastewater at low temperatures.amongst more than 1000 isolates collected in various cold environments, the strain arthrobacter psychrolactophilus sp 31.3 has been selected for its ability to grow and to produce exoenzymes at low temperatures, its inability to grow at 37 degrees c, its non-halophilic character and its growth versatility on various media. this non-pathogenic strain displays a strong resistance to desiccation and storage at room temperature and is suitable for the production of freeze-dried bacterial starters. w ...200919578929
a new cold-adapted beta-d-galactosidase from the antarctic arthrobacter sp. 32c - gene cloning, overexpression, purification and properties.the development of a new cold-active beta-d-galactosidases and microorganisms that efficiently ferment lactose is of high biotechnological interest, particularly for lactose removal in milk and dairy products at low temperatures and for cheese whey bioremediation processes with simultaneous bio-ethanol production.200919631003
arthrobacter psychrochitiniphilus sp. nov., a psychrotrophic bacterium isolated from antarctica.a psychrotrophic arthrobacter strain, gp3(t), was isolated from adélie penguin guano from antarctica and characterized. the 16s rrna gene sequence of the novel strain showed the highest similarity (97.8 %) with that of arthrobacter psychrolactophilus b7(t). the novel strain showed a morphological change from rod to coccus. the growth temperature range of strain gp3(t) was 0-25 degrees c, with optimal growth at 20 degrees c. the strain grew over a salinity range of between 0 and 3 % (w/v) nacl an ...200919625417
monitoring enzyme catalysis in the multimeric state: direct observation of arthrobacter 4-hydroxybenzoyl-coenzyme a thioesterase catalytic complexes using time-resolved electrospray ionization mass spectrometry.the ability to examine real-time reaction kinetics for multimeric enzymes in their native state may offer unique insights into understanding the catalytic mechanism and its interplay with three-dimensional structure. in this study, we have used a time-resolved electrospray mass spectrometry approach to probe the kinetic mechanism of 4-hydroxybenzoyl-coenzyme a (4-hba-coa) thioesterase from arthrobacter sp. strain su in the millisecond time domain. intact tetrameric complexes of 4-hba-coa thioest ...200919635449
genetic diversity of plant growth promoting rhizobacteria isolated from rhizospheric soil of wheat under saline condition.in this study, a total of 130 rhizobacteria was isolated from a saline infested zone of wheat rhizosphere, and screened for plant growth promoting (pgp) traits at higher salt (nacl) concentrations (2, 4, 6, and 8%). the results revealed that 24 rhizobacterial isolates were tolerant at 8% nacl. although all the 24 salt tolerable isolates produced indole-3-acetic acid (iaa), while 10 isolates solubilized phosphorus, eight produced siderophore, and six produced gibberellin. however, only three isol ...200919701667
atrazine and terbuthylazine mineralization by an arthrobacter sp. isolated from a sugarcane-cultivated soil in kenya.a tropical soil from a kenyan sugarcane-cultivated field showed a very high capability to mineralize (14)c-ring-labeled atrazine. in laboratory experiments this soil mineralized about 90% of the applied atrazine within 98 d. the atrazine-degrading microbial community was enriched in liquid cultures containing atrazine as the sole n source and 100 mgl(-1) glucose as additional c source. from the enrichment culture a bacterial strain was isolated and identified by comparative sequence analysis of ...200919674769
effects of bacterial treatment at early stages of atlantic cod (gadus morhua l.) on larval survival and development.to assess the effects of bacterial treatment at the earliest stages of cod rearing on the microbial load, larval development and performance, testing three bacterial strains (carnobacterium divergens v41, arthrobacter sp. and enterococcus sp.) in vivo that were previously shown to have inhibitory potential towards fish pathogens in vitro.201019664064
the presence of embedded bacterial pure cultures in agar plates stimulate the culturability of soil bacteria.traditional methods for bacterial cultivation recover only a small fraction of bacteria from all sorts of natural environments, and attempts have been made to improve the bacterial culturability. here we describe the development of a cultivation method, based on the embedment of pure bacterial cultures in between two layers of agar. plates containing either embedded pseudomonas putida or arthrobacter globiformis resulted in higher numbers of cfus of soil bacteria (21% and 38%, respectively) afte ...200919699242
impact of 4-chlorophenol contamination and/or inoculation with the 4-chlorophenol-degrading strain, arthrobacter chlorophenolicus a6l, on soil bacterial community structure.the 4-chlorophenol-degrading strain, arthrobacter chlorophenolicus a6l (chromosomally tagged with the firefly luciferase gene, luc) was inoculated into 4-chlorophenol-contaminated soil to assess the impact of bioaugmentation with a biodegrading strain on the indigenous microbiota. simultaneously, the impact of 4-chlorophenol alone, or inoculation with a. chlorophenolicus into non-contaminated soil, was addressed. using terminal restriction fragment length polymorphism (t-rflp) several significan ...200219709298
eric-pcr-based strain-specific detection of phenol-degrading bacteria in activated sludge of wastewater treatment systems.to evaluate the use of enterobacterial repetitive intergenic consensus pcr (eric-pcr)-derived probes and primers to specifically detect bacterial strains in an activated sludge microbial community.200919708883
plasmid localisation of atrazine-degrading genes in newly described chelatobacter and arthrobacter strains.abstract in a previous study, we isolated a collection of atrazine-degrading bacteria from various soils. the aim of this study was to localise the atrazine-degrading genes in these 25 atrazine-degrading strains. in the case of the gram-negative strains of chelatobacter heintzii, six to seven plasmids were observed. the atzabc and trzd genes were located on two or three plasmids with variable molecular masses. for the gram-positive strains of arthrobacter crystallopoietes, the atzbc genes were l ...200219709240
bacterial populations associated with mycelium of the arbuscular mycorrhizal fungus glomus intraradices.abstract the influences of the arbuscular mycorrhizal fungus glomus intraradices on the culturable aerobic-heterotrophic bacterial communities in the rhizosphere and hyphosphere of cucumber plants (cucumis satvius) were investigated. mycorrhizal and non-mycorrhizal plants were grown in compartmentalised growth units, each with a root compartment and two lateral root-free compartments. samples representing rhizosphere, root-free soil, root-free sand and washed sand extract were collected 52 days ...200219709247
genetic and physiological diversity of phylogenetically and geographically distinct groups of arthrobacter isolated from lead-zinc mine tailings.the phylogenetic positions of 60 bacterial strains isolated from three tailing piles were determined by analyzing their partial 16s rrna gene sequences. these strains were divided into three phylogenetically distinct groups of arthrobacter and likely represented several non-described species. the physiological diversities of these phylogenetically and geographically distinct populations were evaluated based on their resistance to five heavy metals (zn, pb, cd, cu and co) and four antibiotics (ka ...200419712425
antarctic bacteria inhibit growth of food-borne microorganisms at low temperatures.the aim of this study was to identify antarctic microorganisms with the ability to produce cold-active antimicrobial compounds with potential for use in chilled food preservation. colonies (4496) were isolated from 12 antarctic soil samples and tested against listeria innocua, pseudomonas fragi and brochothrix thermosphacta. thirteen bacteria were confirmed as being growth-inhibitor producers (detection rate 0.29%). when tested against a wider spectrum of eight target organisms, some of the isol ...200419712399
impact of phenolic substrate and growth temperature on the arthrobacter chlorophenolicus proteome.we compared the arthrobacter chlorophenolicus proteome during growth on 4-chlorophenol, 4-nitrophenol, or phenol at 5 and 28 degrees c, both for the wild-type and a mutant strain with mass spectrometry based proteomics. a label-free workflow employing spectral counting identified 3749 proteins across all growth conditions, representing over 70% of the predicted genome and 739 of these proteins form the core proteome. statistically significant differences were found in the proteomes of cells grow ...200919714879
fluorinated phenylcyclopropylamines. part 6: effects of electron withdrawing or donating aryl substituents on the inhibition of tyramine oxidase from arthrobacter sp. by diastereomeric 2-aryl-2-fluoro-cyclopropylamines.diastereomeric arylcyclopropylamines substituted with fluorine in the 2-position and with electron donating or electron withdrawing groups at the aromatic ring were evaluated as inhibitors of microbial tyramine oxidase. the trans-isomers were consistently more potent inhibitors of the enzyme than the cis-isomers. electron donating substituents increased the potency of tyramine oxidase inhibition, while electron withdrawing substituents decreased the activity. the results obtained are discussed i ...200819727324
[catalytical properties of arthrobacter nicotianae cells, a producer of glucose isomerase, immobilized in xerogel of silicium dioxide].arthrobacter nicotinanae cells, producers of glucose isomerase, were immobilized in xerogel of silicium dioxide, and properties of the resulted heterogeneous biocatalysts were investigated in the process of isomerization of monosaccharide (glucose and fructose). the glucose isomerase activity of the resulted biocatalysts was shown to be 10 u/g, on average, taking into account the loss of the activity upon the immobilization, which amounted to 50% of the cell activity in suspension. the rate of t ...200919764612
a new beta-galactosidase with a low temperature optimum isolated from the antarctic arthrobacter sp. 20b: gene cloning, purification and characterization.a psychrotrophic bacterium producing a cold-adapted beta-galactosidase upon growth at low temperatures was classified as arthrobacter sp. 20b. a genomic dna library of strain 20b introduced into escherichia coli top10f' and screening on x-gal (5-bromo-4-chloro-3-indolyl-beta-d-galactopyranoside)-containing agar plates led to the isolation of beta-galactosidase gene. the beta-galactosidase gene (bgas) encoding a protein of 1,053 amino acids, with a calculated molecular mass of 113,695 kda. analys ...200919771412
isolation and identification of novel microcystin-degrading bacteria.of 31 freshwater bacterial isolates screened using the biolog mt2 assay to determine their metabolism of the microcystin lr, 10 were positive. phylogenetic analysis (16s rrna) identified them as arthrobacter spp., brevibacterium sp., and rhodococcus sp. this is the first report of microcystin degraders that do not belong to the proteobacteria.200919734339
high-level chromate resistance in arthrobacter sp. strain fb24 requires previously uncharacterized accessory genes.the genome of arthrobacter sp. strain fb24 contains a chromate resistance determinant (crd), consisting of a cluster of 8 genes located on a 10.6 kb fragment of a 96 kb plasmid. the crd includes chra, which encodes a putative chromate efflux protein, and three genes with amino acid similarities to the amino and carboxy termini of chrb, a putative regulatory protein. there are also three novel genes that have not been previously associated with chromate resistance in other bacteria; they encode a ...200919758450
the influence of host-guest inclusion complex formation on the biotransformation of cortisone acetate delta(1)-dehydrogenation.an intensive and systematic investigation had been carried out on the delta(1)-dehydrogenation of cortisone acetate (ca) to prednisone acetate (pa) by arthrobacter simplex tccc 11037 in the presence of native and modified beta-cyclodextrins (beta-cds). the biotransformation was improved through the formation of the host-guest inclusion complex between ca and cds in aqueous solution. the inclusion complexes of cds with ca were investigated by means of phase solubility, 2d nmr spectroscopy and dif ...200919744560
soil bacterial communities in constructed wetlands treated with swine wastewater using pcr-dgge technique.marsh-pond-marsh (mpm) constructed wetlands were designed for the treatment of swine wastewater. the goal of this study was to characterize bacterial communities in these wetlands and determine the nutrient removal from influent to effluent. surface soil samples were collected and analyzed by culture-dependent and culture-independent techniques. the results showed that the bacterial colony forming units (cfu) and the average concentrations of total nitrogen, nh(4)(+), total phosphorous (tp) and ...201019822421
[biodehydrogenation of 11beta-hydroxyl melroxyprogesterone by arthrobacter simplex ur016 in microemulsion system].to improve mass transfer and enhance the yield for c(1,2) biodehydrogenation of steroid 11beta-hydroxyl medroxyprogesterone, we carried out the dehydrogenation reaction of 11beta-hydroxyl medroxyprogesterone in an oil-in-water (o/w) microemulsion by arthrobacter simplex ur016. we studied the effects of system composition, dehydrogenation temperature and substrate concentration on microbial transformation. we formulated a suitable o/w microemulsion system with arthrobacter simplex ur016 culture b ...200919777818
arthrobacter antarcticus sp. nov., isolated from an antarctic marine sediment.a bacterial strain, spc26(t), was isolated from a sediment sample of the southern ocean off antarctica. the strain was gram-staining- and catalase-positive and contained lysine and alanine in the cell-wall peptidoglycan. the major cellular fatty acids were anteiso-c₁₅:₀ (54.92 %), iso-c₁₅:₀ (11.47 %), anteiso-c₁₇:₀ (6.48 %) and anteiso-c₁₅:₁ (6.38 %) and the major menaquinones were mk-8, mk-9 and mk-10. the major polar lipids were phosphatidylethanolamine and diphosphatidylglycerol. the g+c cont ...201019783612
[dormant form of micrococcus luteus and arthrobacter globiformis, not growing on standard media]. 200919827710
characteristics and function of alcaligenes sp. nbrc 14130 esterase catalysing the stereo-selective hydrolysis of ethyl chrysanthemate.alcaligenes sp. nbrc 14130 was found as a strain hydrolysing a mixture of (+/-)-trans- and (+/-)-cis ethyl chrysanthemates to (1r,3r)-(+)-trans-chrysanthemic acid. the alcaligenes cells also have hydrolytic activity for 6-aminohexanoate-cyclic dimer (6-ahcd, 1,8-diazacyclotetradecane-2,9-dione). the correlation of function on the enzyme from the alcaligenes strain with hydrolysis activities for both ethyl chrysanthemate and 6-ahcd was demonstrated.201019778353
analysis of two gene clusters involved in the degradation of 4-fluorophenol by arthrobacter sp. strain if1.arthrobacter sp. strain if1 is able to grow on 4-fluorophenol (4-fp) as a sole source of carbon and energy. to clone the 4-fp degradation genes, dna libraries were constructed and screened with a probe obtained by pcr using primers designed on the basis of conserved regions of aromatic two-component monooxygenases. sequencing of positive clones yielded two gene clusters, each harboring a gene encoding a monooxygenase with high sequence similarity to the oxygenase component of 4-nitrophenol and 4 ...200919837837
carbon nanotube clusters as universal bacterial adsorbents and magnetic separation agents.the magnetic susceptibility and high bacterial affinity of carbon nanotube (cnt) clusters highlight their great potential as a magnetic bio-separation agent. this article reports the cnt clusters' capability as "universal" bacterial adsorbents and magnetic separation agents by designing and testing a multiwalled carbon nanotube (mwnt) cluster-based process for bacterial capturing and separation. the reaction system consisted of large clusters of mwnts for bacterial capture and an external magnet ...201019856390
[genetic diversity of cultivable bacteria of dominant plants in a potassium mine tailing of nanjing].plant rhizosphere bacteria play an important role in biogeochemical cycles. microbial diversity of cultivable bacteria from soil-plant-mineral system in a mine tailing was assessed.200919873749
arthrobacter alpinus sp. nov., a psychrophilic bacterium isolated from alpine soil.an aerobic, gram-reaction-positive, non-motile, psychrophilic bacterium, designated strain s6-3(t), was isolated from alpine soil. cells exhibited a rod-coccus growth cycle and produced a yellow pigment. growth occurred at 1-25 degrees c. phylogenetic analysis based on 16s rrna gene sequences showed that strain s6-3(t) was related to members of the genus arthrobacter, sharing highest sequence similarities with the type strains of arthrobacter psychrolactophilus (97.9 %) and arthrobacter stackebr ...201019880631
genetic diversity of phthalic acid esters-degrading bacteria isolated from different geographical regions of china.thirty-two strains of phthalic acid ester (paes)-degrading bacteria were isolated from thirteen geographically diverse sites by enrichment using mixtures of paes as the sole source of carbon and energy. sequence analyses of the 16s rrna gene indicated that these isolates were from six genera (arthrobacter, gordonia, rhodococcus, acinetobacter, pseudomonas, and delftia). to evaluate the genetic diversity among them, the molecular typing method rep-pcr with primers based on enterobacterial repetit ...201019866373
removal of organic compounds by a biofilm supported on gac: modelling of batch and column data.the performance of a biofilm of arthrobacter viscosus supported on granular activated carbon on the retention of organic compounds was evaluated. the presence of functional groups on the cell wall surface of the biomass that may interact with the organic compounds was confirmed by fourier transform infrared spectroscopy, to assess the applicability of this system to the removal of those compounds. the batch assays showed that the removal percentage decreases with the increasing initial concentra ...201019882356
x-ray crystallographic analysis of the 6-aminohexanoate cyclic dimer hydrolase: catalytic mechanism and evolution of an enzyme responsible for nylon-6 byproduct degradation.we performed x-ray crystallographic analyses of the 6-aminohexanoate cyclic dimer (acd) hydrolase (nyla) from arthrobacter sp., an enzyme responsible for the degradation of the nylon-6 industry byproduct. the fold adopted by the 472-amino acid polypeptide generated a compact mixed alpha/beta fold, typically found in the amidase signature superfamily; this fold was especially similar to the fold of glutamyl-trna(gln) amidotransferase subunit a (z score, 49.4) and malonamidase e2 (z score, 44.8). ...201019889645
antagonistic activity of bacteria isolated from crops cultivated in a rotation system and a monoculture against pythium debaryanum and fusarium oxysporum.the effect of crop rotation and monocropping on the occurrence of bacteria with antagonistic activity toward pythium debaryanum and fusarium oxysporum was shown. arthrobacter spp., fluorescent pseudomonas spp. and actinomycetes were isolated from winter rape, sugar beet and winter barley rhizosphere and bulk soil from the plots of a long-term crop rotation experiment (18 years). the occurrence of mycoantagonistic isolates and their antibiosis level exhibited specificity for the site, crop and cr ...200919937218
16s rrna gene-based identification of cultured bacterial flora from host-seeking ixodes ricinus, dermacentor reticulatus and haemaphysalis concinna ticks, vectors of vertebrate pathogens.a total of 151 bacterial isolates were recovered from different developmental stages (larvae, nymphs and adults) of field-collected ticks (67 strains from ixodes ricinus, 38 from dermacentor reticulatus, 46 from haemaphysalis concinna). microorganisms were identified by means of 16s rrna gene sequencing. almost 87 % of the strains belonged to g(+) bacteria with predominantly occurring genera bacillus and paenibacillus. other g(+) strains included arthrobacter, corynebacterium, frigoribacterium, ...200919937215
c and n isotope fractionation suggests similar mechanisms of microbial atrazine transformation despite involvement of different enzymes (atza and trzn).transformation of atrazine to hydroxyatrazine in the environment may be underestimated by current assessment schemes since immobilization and further transformation of the metabolite can render parent-to-daughter compound ratios unreliable. this study reports significant c and n isotope fractionation of atrazine in transformation to hydroxyatrazine by chelatobacter heintzii, pseudomonas sp. adp, and arthrobacter aurescens tc1 highlighting an alternative approach to detecting this natural transfo ...200919924926
[isolation and identification of four dbp-degrading strains and molecular cloning of the degradation genes].four di-butyl-phthalate(dbp)-degrading bacterial strains, jdc-1, jdc-8, jdc-9 and jdc-12, were isolated from soil. the strains were gram positive. the 16s rrna sequence analysis revealed that the four strains had similarities of 99% with arthrobacter sp.. according to the morphologic, physiobiochemical characteristics and the analysis of their 16s rrna, all the four strains were identified as arthrobacter sp.. a 900 bp dna fragment was obtained from the four strains by pcr amplified and clone. w ...200919927831
[dynamics of microbial populations in the biofilm on protective coating].dynamics of microbial populations in the biofilm on the surface of protective coating poliken 980-25 has been studied. it was shown that in the process of succession one can observe some changes of taxonomic composition of biofilm bacteria. bacteria of pseudomonas genus were revealed at early stages of succession, they reduce fe+3 and oxidize carbohydrates. bacteria of arthrobacter genus begin developing 2 hours after the exposure, bacillus genus bacteria appear 6 hours after the experiment begi ...200919938604
[monosaccharide composition of exopolymer complex of bacteria-destructors of protective coatings].monosaccharide composition of exopolymer complex (epc) ofbacteria-destructors of protective coatings pseudomonas sp. t/, pseudomonas sp. 109, arthrobacter sp. 102 has been studied. it is established that monosaccharide composition of exopolymer complex of bacteria is different and depends on the bacteria growth model. it is mainly represented by penthoses and hexoses. glucose, galactose, arabinose, which are determined in epc of biofilm and plankton of all studied bacteria, are dominating monosa ...200919938612
[lipolytic and catalase activity of bacteria-destructors of protective coatings].lipolytic and catalase activity of bacteria-destructors of protective coatings at different growth models: biofilm and plankton have been studied. it was shown that the synthesis of exoenzymes in the biofilm is different. activity of extracellular lipase and catalase in the biofilm is 1.1-1.5 and 1.2-2.1 times higher, respectively, than in plankton. the experiment duration being increased to 14 days, the lipolytic activity considerably decreases. activity of studied enzymes, produced by bacteria ...200919938616
isolation of an isocarbophos-degrading strain of arthrobacter sp. scl-2 and identification of the degradation pathway.isocarbophos is a widely used organophosphorus insecticide that has caused environmental pollution in many areas. however, degradation of isocarbophos by pure cultures has not been extensively studied, and the degradation pathway has not been determined. in this paper, a highly effective isocarbophos-degrading strain, scl-2, was isolated from isocarbophos-polluted soil. strain scl-2 was preliminarily identified as arthrobacter sp. based on its morphological, physiological, and biochemical proper ...200919996699
crystal structure analysis of free and substrate-bound 6-hydroxy-l-nicotine oxidase from arthrobacter nicotinovorans.the pathway for oxidative degradation of nicotine in arthrobacter nicotinovorans includes two genetically and structurally unrelated flavoenzymes, 6-hydroxy-l-nicotine oxidase (6hlno) and 6-hydroxy-d-nicotine oxidase, which act with absolute stereospecificity on the l- and d-forms, respectively, of 6-hydroxy-nicotine. we solved the crystal structure of 6hlno at 1.95 a resolution by combined isomorphous/multiple-wavelength anomalous dispersion phasing. the overall structure of each subunit of the ...201020006620
molecular identification of airborne bacteria associated with aerial spraying of bovine slurry waste employing 16s rrna gene pcr and gene sequencing techniques.polymerase chain reaction amplification of the universal 16s ribosomal rna (rrna) gene was performed on a collection of 38 bacterial isolates, originating from air sampled immediately adjacent to the agricultural spreading of bovine slurry. a total of 16 bacterial genera were identified including both gram-positive and gram-negative genera. gram-positive organisms accounted for 34/38 (89.5%) of total bacterial numbers consisting of 12 genera and included staphylococcus (most common genus isolate ...201020018377
complete degradation of di-n-octyl phthalate by biochemical cooperation between gordonia sp. strain jdc-2 and arthrobacter sp. strain jdc-32 isolated from activated sludge.two bacterial strains were isolated from activated sludge using mixtures of phthalic acid esters (paes) as the sole source of carbon and energy. one of the isolates was identified as gordonia sp. strain jdc-2 and the other as arthrobacter sp. strain jdc-32, mainly through 16s rrna gene sequence analysis. gordonia sp. strain jdc-2 rapidly degraded di-n-octyl phthalate (dop) into phthalic acid (pa), which accumulated in the culture medium. arthrobacter sp. strain jdc-32 degraded pa but not dop. th ...201019959291
a polyphasic study on the species diversity of the sediment microbiota of lake hévíz.lake hévíz is the largest natural thermal lake of europe, harboring special bacterial communities. the aim of the present study was to gain information about the distribution and species diversity of the sediment microbiota, with special focus on actinobacteria, by using cultivation-based and -independent molecular methods. samples from two depths were taken in two different locations in october 2007. 245 strains were isolated, grouped to 85 otus by ardra, and identified by 16s rdna sequencing. ...200920038486
heavy metal resistance in arthrobacter ramosus strain g2 isolated from mercuric salt-contaminated soil.present study describes isolation of a multiple metal-resistant arthrobacter ramosus strain from mercuric salt-contaminated soil. the isolate was found to resist and bioaccumulate several metals, such as cadmium, cobalt, zinc, chromium and mercury. maximum tolerated concentrations for above metals were found to be 37, 525, 348, 1530 and 369 microm, respectively. the isolate could also reduce and detoxify redox-active metals like chromium and mercury, indicating that it has great potential in bio ...201020060643
enhanced cyclic adenosine monophosphate production by arthrobacter a302 through rational redistribution of metabolic flux.cyclic adenosine monophosphate (camp) was synthesized through the purine salvage synthesis pathway by arthrobacter a302. results showed that hypoxanthine was the best of the precursors, and the camp concentration reached 4.06 g/l. for inhibition of the glycolytic pathway, sodium fluoride was found the optimal effector, which was further studied on camp production. with the addition of 0.4 g/l of sodium fluoride, the maximal camp concentration reached 11.04 g/l, and the concentrations of lactic a ...201020064709
genetic and phenotypic diversity of parathion-degrading bacteria isolated from rice paddy soils.three parathion-degrading bacteria and eight pairs of bacteria showing syntrophic metabolism of parathion were isolated from rice field soils, and their genetic and phenotypic characteristics were investigated. the three isolates and eight syntrophic pairs were able to utilize parathion as a sole source of carbon and energy, producing p-nitrophenol as the intermediate metabolite during the complete degradation of parathion. analysis of 16s rrna gene sequence indicated that the isolates were rela ...200920075637
growth of arthrobacter sp. strain jbh1 on nitroglycerin as the sole source of carbon and nitrogen.arthrobacter sp. strain jbh1 was isolated from nitroglycerin-contaminated soil by selective enrichment. detection of transient intermediates and simultaneous adaptation studies with potential intermediates indicated that the degradation pathway involves the conversion of nitroglycerin to glycerol via 1,2-dinitroglycerin and 1-mononitroglycerin, with concomitant release of nitrite. glycerol then serves as the source of carbon and energy.201020061454
structural basis for cofactor-independent dioxygenation of n-heteroaromatic compounds at the alpha/beta-hydrolase fold.enzymatic catalysis of oxygenation reactions in the absence of metal or organic cofactors is a considerable biochemical challenge. the co-forming 1-h-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase (hod) from arthrobacter nitroguajacolicus rü61a and 1-h-3-hydroxy-4-oxoquinoline 2,4-dioxygenase (qdo) from pseudomonas putida 33/1 are homologous cofactor-independent dioxygenases involved in the breakdown of n-heteroaromatic compounds. to date, they are the only dioxygenases suggested to belong to the alp ...201020080731
phylogenetic diversity of alkaline protease-producing psychrotrophic bacteria from glacier and cold environments of lahaul and spiti, india.the diversity of proteolytic bacteria associated with a glacier and cold environment soils from three different locations in lahaul and spiti, india was investigated. two hundred seventeen bacterial strains were isolated in pure culture. subsequently these strains were screened for protease-production and one hundred nine showed protease production. from these protease producing psychrotrophic bacteria twenty showing high enzyme production at low temperature and alkaline ph were characterized an ...201020082368
biotransformation of nicotine by microorganism: the case of pseudomonas spp.several bacterial species are capable of using nicotine, the main alkaloid in tobacco plants, as a substrate for growth. the dominant species include members of two genera, pseudomonas and arthrobacter. the degradation pathway and genetic structure of nicotine catabolism in arthrobacter nicotinovorans were recently reviewed (brandsch appl microbiol biotechnol 69:493-498, 2006). here, we present up-to-date information on biodegradation of nicotine by pseudomonas spp. species in this genus capable ...201020091027
influence of internal biofilm growth on residual permeability loss in aerobic granular membrane bioreactors.membrane fouling results in flux decline or transmembrane pressure drop increase during membrane bioreactor (mbr) operation. physical and chemical cleanings are essential to keep an mbr operating at an appropriate membrane flux. considerable residual membrane permeability loss that cannot be removed by conventional cleaning requires membrane replacement. this study demonstrates that an internal biofilm can develop inside a hollow-fiber membrane and can probably account for up to 58.9 and 81.3% o ...201020102183
identification and characterization of bacteria isolated under selective pressure of volatile organic compounds.several bacterial strains tolerant to volatile organic compounds (vocs) were isolated from the air emissions of a typical industrial wood painting plant. four strains that grew vigorously on a mineral salt basal medium and voc-saturated atmosphere were identified using biolog and fatty acids analysis. a 16s rdna sequence comparison was also performed on three of the four strains. their phylogenetic positions were respectively: mc8m6 of the genus arthrobacter mcep3 of the genus microbacterium, mc ...200920112870
copy number determination, expression analysis of genes potentially involved in replication, and stability assays of pal1 - the linear megaplasmid of arthrobacter nitroguajacolicus rü61a.pal1 of the actinomycete arthrobacter nitroguajacolicus rü61a, a 113-kb catabolic linear megaplasmid of the invertron-type, enables its host to utilize quinaldine (or 4-hydroxyquinaldine) as the sole carbon and energy source. comparative southern hybridization and quantitative real-time pcr analyses revealed copy numbers of two for cells grown in minimal medium supplemented with 4-hydroxyquinaldine and four for cells cultivated in luria-bertani medium. however, medium-dependent differences in th ...201120116226
mechanism of boron tolerance in soil bacteria.boron (b) is toxic to living cells at levels above a certain threshold. we isolated several b-tolerant bacterial strains from soil samples and studied them for possible mechanisms of b tolerance. 16s rrna gene sequencing and comparative phylogenetic analysis demonstrated that the isolates belong to the following 6 genera: arthrobacter, rhodococcus, lysinibacillus, algoriphagus, gracilibacillus, and bacillus. these isolates exhibited b-tolerance levels of 80, 100, 150, 300, 450, and 450 mmol/l, r ...201020130690
biosurfactants, bioemulsifiers and exopolysaccharides from marine microorganisms.marine biosphere offers wealthy flora and fauna, which represents a vast natural resource of imperative functional commercial grade products. among the various bioactive compounds, biosurfactant (bs)/bioemulsifiers (be) are attracting major interest and attention due to their structural and functional diversity. the versatile properties of surface active molecules find numerous applications in various industries. marine microorganisms such as acinetobacter, arthrobacter, pseudomonas, halomonas, ...201020172021
the biomass yielding process of xenobiotic degradation.yields of activated sludge and an arthrobacter sp. biomass on organic xenobiotic 2,4-dichlorophenoxyacetic acid (2,4-d) and on the intermediates of selected 2,4-d metabolism pathways were measured. activated sludge yield on 2,4-d was lower by approximately 24-45% compared to the combined yields produced separately by the lower intermediates. for activated sludge, cell synthesis only consumed 33% of the electrons generated from 2,4-d oxidation, while the other 67% were used for energy. the high e ...201020153175
identification and in vitro deoxynucleotidylation of the terminal protein of the linear plasmid pal1 of arthrobacter nitroguajacolicus rü61a.the plasmid pal1 of arthrobacter nitroguajacolicus rü61a is a linear replicon, characterized by inverted terminal repeats and terminal proteins (tps) covalently bound to its 5'-ends. previous sequence analysis and predictions of possible secondary structures formed by telomeric 3'-overhangs indicated significant differences of the 'left' and 'right' telomere of pal1, raising the question of whether each terminus is recognized by a specific protein. the genes pal1.102 and pal1.103, located close ...201020141532
one-pot conversion of levan prepared from serratia levanicum nn to difructose anhydride iv by arthrobacter nicotinovorans levan fructotransferase.the newly established difructose anhydride iv (dfa iv) production system is comprised of the effective production of levan from sucrose by serratia levanicum nn, the conversion of the levan into dfa iv by levan fructotransferase from arthrobacter nicotinovorans gs-9, which is highly expressed in an escherichiacoli transformant, and a practical purification step. the chemical properties of dfa iv were also investigated.201020159571
identification of airborne bacterial and fungal species in the clinical microbiology laboratory of a university teaching hospital employing ribosomal dna (rdna) pcr and gene sequencing techniques.universal or "broad-range" pcr-based ribosomal dna (rdna) was performed on a collection of 58 isolates (n = 30 bacteria + 28 fungi), originating from environmental air from several locations within a busy clinical microbiology laboratory, supporting a university teaching hospital. a total of 10 bacterial genera were identified including both gram-positive and gram-negative genera. gram-positive organisms accounted for 27/30 (90%) of total bacterial species, consisting of seven genera and include ...200920183192
strains of internal biofilm in aerobic granular membrane bioreactors.this study isolated strains in suspended liquor, the surface fouling layer, and biofilm inside hollow-fiber membranes of a membrane bioreactor (mbr); analyzed their distributions, sizes, surface charges, and growth behaviors; and determined the quantities of extracellular polymeric substances (eps) secreted by these strains under different organic loadings. three strains, which may penetrate the microfiltration membranes, were close relatives of the ralstonia mannitolilytica strain sdv (genbank ...201020306189
c, n, and h isotope fractionation of the herbicide isoproturon reflects different microbial transformation pathways.the fate of pesticides in the subsurface is of great interest to the public, industry, and regulatory authorities. compound-specific isotope analysis (csia) is a promising tool complementary to existing methods for elucidating pesticide degradation reactions. here, we address three different initial biotransformation reactions of the phenylurea herbicide isoproturon (3-(4-isopropylphenyl)-1,1-dimethylurea) in pure culture experiments with bacterial and fungal strains. when analyzing isotopic cha ...201020184312
role of valine 464 in the flavin oxidation reaction catalyzed by choline oxidase.the oxidation of reduced flavin cofactors by oxygen is a very important reaction that is central to the chemical versatility of hundreds of flavoproteins classified as monooxygenases and oxidases. these enzymes are characterized by bimolecular rate constants >or=10(5) m(-1) s(-1) and produce water and hydrogen peroxide, respectively. a hydrophobic cavity close to the reactive flavin c(4a) atom has been previously identified in the 3d structure of monooxygenases but not in flavoprotein oxidases. ...201020184377
esmond e. snell--the pathfinder of b vitamins and cofactors.esmond e. snell (1914-2003) was a giant of b-vitamin and enzyme research. his early research in bacterial nutrition had lead to the discovery of vitamins such as lipoic acid and folic acid, and an anti-vitamin avidin. he developed microbiological assay methods for riboflavin and other vitamins and amino acids, which are still used today. he also investigated the metabolism of vitamins, discovered pyridoxal and pyridoxamine as the active forms of vitamin b(6) and revealed the mechanism of transam ...201020353954
crystallization and preliminary crystallographic analysis of manganese(ii)-dependent 2,3-dihydroxybiphenyl 1,2-dioxygenase from bacillus sp. jf8.a thermostable manganese(ii)-dependent 2,3-dihydroxybiphenyl-1,2-dioxygenase derived from bacillus sp. jf8 was crystallized. the initial screening for crystallization was performed by the sitting-drop vapour-diffusion method using a crystallization robot, resulting in the growth of two crystal forms. the first crystal belonged to space group p1, with unit-cell parameters a = 62.7, b = 71.4, c = 93.6 a, alpha = 71.2, beta = 81.0, gamma = 64.0 degrees, and diffracted to 1.3 a resolution. the secon ...201020208161
arthrobacter sp. strain ku001 isolated from a thai soil degrades atrazine in the presence of inorganic nitrogen sources.an atrazine degrading bacterium, strain ku001, was obtained from a sugarcane field at the cane and sugar research and development center at the kasetsart university, kamphaeng saen campus, thailand. strain ku001 had a rod-to-coccus morphological cycle during growth. biolog carbon source analysis indicated that the isolated bacterium was arthrobacter histidinolovorans. sequence analysis of pcr product indicated the 16s rrna gene in strain ku001 was 99% identical to the same region in arthrobacter ...201020372034
aerobic biodegradation of a sulfonated phenylazonaphthol dye by a bacterial community immobilized in a multistage packed-bed bac reactor.a microbial community able to aerobically degrade the azo dye acid orange 7 was selected from riparian or lacustrine sediments collected at sites receiving textile wastewaters. three bacterial strains, pertaining to the genera pseudomonas, arthrobacter, and rhizobium, constitute the selected community. the biodegradation of ao7 was carried out in batch-suspended cell culture and in a continuously operated multistage packed-bed bac reactor. the rapid decolorization observed in batch culture, join ...201020376575
cg2091 encodes a polyphosphate/atp-dependent glucokinase of corynebacterium glutamicum.the corynebacterium glutamicum gene cg2091 is encoding a polyphosphate (polyp)/atp-dependent glucokinase (ppgk). previous work demonstrated the association of ppgk to polyp granules. the deduced amino acid sequence of ppgk shows 45% sequence identity to polyp/atp glucomannokinase of arthrobacter sp. strain km and 50% sequence identity to polyp glucokinase of mycobacterium tuberculosis h37rv. ppgk from c. glutamicum was purified from recombinant escherichia coli. polyp was highly preferred over a ...201020379711
[isolation and identification of a fibrinolytic bacterium strain from frozen soil in the tibetan plateau].cardiovascular disease, harmful to human health, is a worldwide disease, for which fibrinolytic enzyme is usually used as an effective remedy. fibrinolytic microbes under extreme environmental conditions may excrete some high efficient and safe fibrinolytic enzymes. so, for obtaining this fibrinolytic enzyme, we isolated fibrinolytic strains from frozen soil in the tibetan plateau.201020387455
structural characterization of the major glycolipids from arthrobacter globiformis and arthrobacter scleromae.arthrobacter is a genus of gram-positive bacteria widely distributed in soil. the ability to catabolize a variety of xenobiotics has shown their potential as a detoxifying agent. recently, arthrobacter has been also recognized as an opportunistic pathogen. glycolipids from a. scleromae, a clinical isolate, and a. globiformis, from soil, were isolated by chloroform-methanol extraction and subsequently purified using column chromatography and high-performance liquid chromatography. structural stud ...201020381794
culture-dependent and culture-independent diversity of actinobacteria associated with the marine sponge hymeniacidon perleve from the south china sea.in this report, the diversity of actinobacteria associated with the marine sponge hymeniacidon perleve collected from a remote island of the south china sea was investigated employing classical cultivation and characterization, 16s rdna library construction, 16s rdna-restriction fragment length polymorphism (rdna-rflp) and phylogenetic analysis. a total of 184 strains were isolated using seven different media and 24 isolates were selected according to their morphological characteristics for phyl ...201020383659
aepa of pectobacterium is not involved in the regulation of extracellular plant cell wall degrading enzymes production.plant cell wall degrading enzymes (pcwde) are the major virulence determinants in phytopathogenic pectobacterium, and their production is controlled by many regulatory factors. in this study, we focus on the role of the aepa protein, which was previously described to be a global regulator of pcwde production in pectobacterium carotovorum (murata et al. in mol plant microbe interact 4:239-246, 1991). our results show that neither inactivation nor overexpression of aepa affects pcwde production in ...201020386924
glycosylation patterns of human alpha2-macroglobulin: analysis of lectin binding by electron microscopy.human alpha2-macroglobulin (alpha 2m) is a 720 kda glycoprotein that presents two ultrastructural conformations: slow (s-alpha 2m) and fast (f-alpha 2m). alpha 2m acts mainly as a proteinase scavenger, but an immunomodulatory role was also proposed. this work studies the effect of desialylation and deglycosylation on the structure patterns of alpha 2m by ultrastructural analysis of lectin-induced aggregates, which represents a new approach that had never been previously used. transmission electr ...201020399104
comparative study of properties of immobilized lipase onto glutaraldehyde-activated amino-silica gel via different methods.the enzyme-aggregate coating method was performed to immobilize arthrobacter sp. lipase in order to achieve better catalytic properties comparable to the conventional covalent attachment and covalent attachment plus cross-linking. the glutaraldehyde-activated amino-silica gel which was synthesized by sol-gel technique was used as the support, and the catalytic characteristics of the lipase preparations were tested in the asymmetric acylation of 4-hydroxy-3-methyl-2-(2-propenyl)-2-cyclopenten-1-o ...201020399626
homology modeling and docking study of xanthine oxidase of arthrobacter sp. xl26.hyperuricemia is a condition of defective purine metabolism characterized with elevated serum uric acid (ua) level that further leads to gout and gouty nephrolithiasis disorders. gout is a world wide distributed rheumatic disease comprises 1% of the total population and still is in increasing state. one of the factors contributing to overproduction of ua is the hydroxylation of xanthine catalyzed by xanthine oxidase (xo). in the present study, 3d modeling of arthrobacter sp. xl26 (xodb) protein ...201020403378
rescuing of the hydride transfer reaction in the glu312asp variant of choline oxidase by a substrate analogue.in the active site of choline oxidase, glu312 participates in binding the trimethylammonium group of choline, thereby positioning the alcohol substrate properly for efficient hydride transfer to the enzyme-bound flavin. previous studies have shown that substitution of glu312 with aspartate results in a perturbed mechanism of hydride transfer, with a 260-fold decrease in the rate associated with the mutation. here, the reaction of alcohol oxidation catalyzed by the glu312asp enzyme has been inves ...201020447376
the microbe-mineral environment and gypsum neogenesis in a weathered polar evaporite.evaporitic deposits are a globally widespread habitat for micro-organisms. the microbe-mineral environment in weathered and remobilized gypsum from exposed mid-ordovician marine evaporite beds in the polar desert of devon island, nunavut, canadian high arctic was examined. the gypsum is characterized by internal green zones of cyanobacterial colonization (dominated by gloeocapsa/aphanothece and chroococcidiopsis spp. morphotypes) and abundant black zones, visible from the surface, that contain p ...201020456500
the type of cheese curds determined the colouring capacity of brevibacterium and arthrobacter species.this study compares the colouring capacity of brevibacterium aurantiacum (ba), brevibacterium bl and arthrobacter species as in relation to deacidified media made from lactic curd (epoisses), mixed curds (munster) and rennet curds (livarot or reblochon). ba colouring capacity proved to be constant, leading to a dark orange colour, irrespective of the deacidified media. however, it gave too dark a colour for reblochon. the strains bl and as were not adapted to the colouring of epoisses deacidifie ...201020462468
calcite-forming bacteria for compressive strength improvement in mortar.microbiological calcium carbonate precipitation (mcp) has been investigated for its ability to improve the compressive strength of concrete mortar. however, very few studies have been conducted on the use of calcite-forming bacteria (cfb) to improve compressive strength. in this study, we discovered new bacterial genera that are capable of improving the compressive strength of concrete mortar. we isolated 4 cfb from 7 environmental concrete structures. using sequence analysis of the 16s rrna gen ...201020467254
engineering of choline oxidase from arthrobacter nicotianae for potential use as biological bleach in detergents.in order to engineer the choline oxidase from arthrobacter nicotianae (an_coda) for the potential application as biological bleach in detergents, the specific activity of the enzyme toward the synthetic substrate tris-(2-hydroxyethyl)-methylammonium methylsulfate (mtea) was improved by methods of directed evolution and rational design. the best mutants (up to 520% wt-activity with mtea) revealed mutations in the fad- (a21v, g62d, i69v) and substrate-binding site (s348l, v349l, f351y). in a separ ...201020467737
arthrobacter endo-beta-n-acetylglucosaminidase shows transglycosylation activity on complex-type n-glycan oxazolines: one-pot conversion of ribonuclease b to sialylated ribonuclease c. 201020486148
[investigation of biotechnological strains of microorganisms for their hygienic standardization].by analyzing the findings and databases on the hygienic standardization of industrial microorganisms, the authors have improved a research program for industrial strains on substantiating their safe exposure levels in workplace and ambient airs. a shortened program is proposed to investigate gram-positive bacteria and nocardioformic actinomycetes (lactobacillus, micrococcus, propionibacterim, brevibacterim, rhodococcus), which have no negative effect. it is necessary to study gram-negative bacte ...201020491276
influence of ph and inorganic phosphate on toxicity of zinc to arthrobacter sp. isolated from heavy-metal-contaminated sediments.because of its high solubility over a wide range of ph conditions, zinc is found in many natural and human-impacted systems. zinc speciation is critical in assessing zinc toxicity to microorganisms because it varies considerably with ph and is dependent on other aqueous constituents. combined results of thermodynamic modeling, statistical analysis, and batch culture studies using arthrobacter sp. jm018 suggest that the toxic species may not be solely limited to the free ion, but also includes zn ...201020553043
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