PMID(sorted descending)
indigoidine and other bacterial pigments related to 3,3'-bipyridyl. 196514347925
microorganisms in the root zone in relation to soil moisture. 196514346123
studies of the lipids of arthrobacter globiformis 616. i. the fatty acid composition. 196514332529
lysis of bacterial cell walls by an enzyme isolated from a myxobacter.ensign, j. c. (university of illinois, urbana), and r. s. wolfe. lysis of bacterial cell walls by an enzyme isolated from a myxobacter. j. bacteriol. 90:395-402. 1965.-an exoenzyme which lyses intact cells, heat-killed cells, and cell walls of arthrobacter crystallopoietes was purified 60-fold from the growth liquor obtained from a myxobacter (strain al-1). the lytic enzyme was produced during growth of the organism in a number of complex media, the maximal amount of enzyme being produced in yea ...196514330733
microbial degradation of isopropyl-n-3 -chlorophenylcarbamate and 2-chloroethyl-n-3-chlorophenylcarbamate.microbial degradation of isopropyl-n-3-chlorophenylcarbamate (cipc) and 2-chloroethyl-n-3-chlorophenylcarbamate (cepc) was observed in a soil perfusion system. degradation in perfused soils, and by pure cultures of effective bacterial isolates, was demonstrated by the production of 3-chloroaniline and the subsequent liberation of free chloride ion. identified isolates effective in degrading and utilizing cipc as a sole source of carbon included pseudomonas striata chester, a flavobacterium sp., ...196514325285
fate of bacteria in chicken meat during freeze-dehydration, rehydration, and plate counts were determined on boneless cooked, cubed chicken meat obtained from a commercial processor. survival of the natural flora was determined after the meat was freeze-dehydrated and rehydrated at room temperature for 30 min and 50, 85, and 100 c for 10 min. total counts of bacteria in the rehydrated samples were determined during storage of the meat at 4, 22, and 37 c until spoilage odor was detectable. meat samples were inoculated with staphylococcus aureus, then dried, rehydrat ...196514325271
biosynthesis of bacterial glycogen. ii. purification and properties of the adenosine diphosphoglucose:glycogen transglucosylase of arthrobacter species nrrl b1973. 196514304835
biosynthesis of bacterial glycogen. i. purification and properties of the adenosine diphosphoglucose pyrophosphorylase of arthrobacter species nrrl b1973. 196514304834
synthetic metal chelators which replace the natural growth-factor requirements of arthrobacter terregens. 196514291610
studies on the biochemical basis of the minimum temperatures for growth of certain psychrophilic and mesophilic micro-organisms. 196514283028
the activation of escherichia coli adp-glucose pyrophosphorylase. 196514282015
microbial degradation of 2,2-dichloropropionic acid in five soils. 196414272481
the metabolism of coumarin by a microorganism. ii. the reduction of o-coumaric acid to melilotic acid. 196414269315
the occurrence of adenosine diphosphate glucose: glycogen transglucosylase in bacteria. 196414247687
sugar nucleotide reactions in arthrobacter. ii. biosynthesis of guanosine diphosphomannuronate. 196414245351
sugar nucleotide reactions in arthrobacter. i. guanosine diphosphate mannose pyrophosphorylase: purification and properties. 196414245350
some studies on trichloroacetate-decomposing soil bacteria. 196414245149
metabolism of coumarin by a micro-organism: o-coumaric acid as an intermediate between coumarin and melilotic acid. 196414243380
specificity of improved methods for mycobactin bioassay by arthrobacter terregens.antoine, alan d. (johns hopkins university-leonard wood memorial leprosy research laboratory, baltimore, md.), norman e. morrison, and john h. hanks. specificity of improved methods for mycobactin bioassay by arthrobacter terregens. j. bacteriol. 88:1672-1677. 1964.-arthrobacter terregens was used to assay mycobactin, a growth factor for mycobacterium paratuberculosis. improved techniques permit the assay of mycobactin within 3 to 4 days by agarplate or liquid-medium methods. for the agarplate m ...196414240956
metabolism of ethanolamine. an ethanolamine oxidase. 196414209947
adenosine diphosphate glucose-glycogen transglucosylase in arthrobacter sp. nrrl b 1973. 196414205498
metabolism of coumarin by a micro-organism. 196414195065
use of arthrobacter terregens for bioassay of mycobactin.reich, claude v. (johns hopkins-leonard wood memorial leprosy research laboratory, johns hopkins university, baltimore, md.), and john h. hanks. use of arthrobacter terregens for bioassay of mycobactin. j. bacteriol. 87:1317-1320. 1964.-arthrobacter terregens was used to assay mycobactin, a growth factor for mycobacterium paratuberculosis. within 7 days, a. terregens gave a linear photometric growth response to mycobactin in the range of 0.05 to 0.2 mug/ml. preparations found to be active (or in ...196414188708
an antibiotic produced by the mycorrhizal fungus cenococcum graniforme. 196414187005
branching enzyme of arthrobacter globiformis. 196414171890
nutritional control of morphogenesis in arthrobacter crystallopietes.ensign, jerald c. (university of illinois, urbana), and r. s. wolfe. nutritional control of morphogenesis in arthrobacter crystallopoietes. j. bacteriol. 87:924-932. 1964.-arthrobacter crystallopoietes exhibits the cyclic, morphological variation which is a characteristic of this genus. a simple chemically defined medium was developed in which this organism is restricted to growth and division entirely in the coccoid form. addition singly to this medium of l-arginine, l-phenylalanine, l-asparagi ...196414137632
morphological aberration of arthrobacter globiformis cells due to biotin deficiency.chan, e. c. s. (university of new brunswick, fredericton, new brunswick, canada). morphological aberration of arthrobacter globiformis cells due to biotin deficiency. j. bacteriol. 87:641-651. 1964.-morphological aberration of arthrobacter globiformis strain 425 was shown to occur during growth in a chemically defined medium without added biotin. such aberrant cells could revert back to normal coccoid forms upon inoculation into fresh medium supplemented with the vitamin. this abnormal cellular ...196414127583
a crystalline pigment produced from 2-hydroxypyridine by arthrobacter crystallopoietes n.sp. 196314106078
new bacterial polysaccharide from arthrobacter.a bacterial strain (nrrl b-1973) isolated from soil at guatemala city and tentatively identified as an arthrobacter species produced a polysaccharide with unusual properties. conditions were studied for the production of this microbial gum in shaken flasks and 20-liter fermentors. suitable nutrients for optimal polysaccharide production included 3% glucose, 0.3% enzyme-hydrolyzed casein, magnesium sulfate, manganese sulfate, and potassium phosphate buffer (ph 7.0). polysaccharide yields ranged f ...196314075048
the assimilation of 2-c compounds other than acetate. 196314053263
[research on various species of nitrogen-fixing arthrobacter isolated from karstic alpine rocks]. 196314048724
[attempt at identification of 2 strains of arthrobacter by study of their antigenic properties and their resistance to antibiotics]. 196314048720
investigations on the action of the iron-containing growth factors, sideramines; and iron-containing antibiotics, sideromycins. 196314045511
on the origin of v-forms in arthrobacter atrocyaneus. 196214039490
production of a gibberellin-like substance by arthrobacter globiformis. 196214031348
a preliminary report on the fine structure of a bacteriophage of arthrobacter polychromogenes schippers-lammertse, muysers et klatser-oedekerk. 196314024588
comparative carbohydrate catabolism in arthrobacter. 196214003016
glutamic acid production by arthrobacter globiformis. 196313996592
metabolism of glycerol by an arthrobacter species. 196213977220
phosphorylation of shikimic acid by ultrasonic extracts of micro-organisms. 196213944994
nicotine oxidation by arthrobacter. 196113901850
[6-hydroxy-pyridyl-(3)]-ketone]. 196113901804
decomposition of puromycin aminoncleoside by arthrobacter flavescens. 196213901476
a ferrichrome-requiring arthrobacter which decomposes puromycin aminonucleoside. 196213901475
chemical composition and antigenic structure of cell walls of corynebacterium, mycobacterium, nocardia, actinomyces and arthrobacter. 196213882624
on the biotin requirement of arthrobacter globiformis. 196213878002
growth interactions of arthrobacter globiformis and pseudomonas sp. in relation to the rhizosphere effect. 196113878001
bacterial iron metabolism: investigations on the mechanism of ferrichrome function. 196213874972
influence of a bacterial cell extract upon the morphogenesis of arthrobacter ureafaciens.blankenship, l. c. (university of maryland, college park) and r. n. doetsch. influence of a bacterial cell extract upon the morphogenesis of arthrobacter ureafaciens. j. bacteriol. 82:882-888. 1961-the effect of a bacterial extract on alleviating the abnormal morphological appearance of arthrobacter ureafaciens when cultivated in thiotone broth is described. physical and chemical analyses of this extract revealed that the mineral components therein were largely responsible for the observed effec ...196113869842
arthrobacter atrocyaneus, n. sp., and its blue pigment. 196013856051
[on the decomposition of nicotine by bacterial enzymes. iii. metabolic studies on cell free extracts]. 196113721112
[on the degradation of nicotine by bacterial enzymes]. 196013721111
[on the decomposition of nicotine by bacterial enzymes. iv. l-6-hydroxynicotine as the first intermediate product]. 196113721110
[on the decomposition of nicotine by bacterial enzymes. ii. isolation and characterization of a nicotine-splitting soil bacterium]. 196113696685
studies on the metabolic function of the ferrichrome compounds. 196113689181
morphological comparison of some arthrobacter species. 195813536906
observations on the morphogenesis of arthrobacter citreus, spec nov. 195413143001
arthrobacter koreensis sp. nov., a novel alkalitolerant bacterium from soil.two arthrobacter strains, ca15-8(t) and ca15-9, were isolated from soil, under alkali conditions, and characterized phenotypically, chemotaxonomically and genetically. these alkalitolerant organisms grew over a wide ph range (ph 7.0-12.0), with an optimum at ph 7.0-8.0. the mean g+c content of the dna of these strains was 63+/-2 mol%. the strains contained mk-8(h(2)) and mk-9(h(2)) as the main respiratory quinones. the cell-wall peptidoglycan was lys-thr-ala(2) and the whole-cell sugar was rhamn ...200313130006
inhibition of beta-fructofuranosidases and alpha-glucosidases by synthetic thio-fructofuranoside.a synthetic beta-thio-fructofuranoside of mercaptoethanol inhibited not only beta-fructofuranosidases but also alpha-glucosidases. the compound was hardly hydrolyzed by the glycosidases. the thio-fructoside competitively inhibited beta-fructofuranosidases from aspergillus niger, candida sp., and saccharomyces cerevisiae, but not arthrobacter beta-fructofuranosidase at all. sucrase activity of rat intestinal sucrase/isomaltase complex was also suppressed in the presence of the thio-fructoside. th ...200312951505
biochemical characterization of a beta-galactosidase with a low temperature optimum obtained from an antarctic arthrobacter isolate.a psychrophilic gram-positive isolate was obtained from antarctic dry valley soil. it utilized lactose, had a rod-coccus cycle, and contained lysine as the diamino acid in its cell wall. consistent with these physiological traits, the 16s ribosomal dna sequence showed that it was phylogenetically related to other arthrobacter species. a gene (bgas) encoding a family 2 beta-galactosidase was cloned from this organism into an escherichia coli host. preliminary results showed that the enzyme was co ...200312949099
crystallization and preliminary x-ray analysis of inorganic polyphosphate/atp-glucomannokinase from arthrobacter sp. strain km.inorganic polyphosphate [poly(p)]/atp-glucomannokinase from arthrobacter sp. strain km phosphorylates glucose and mannose, utilizing both atp and poly(p) as phosphoryl donors. the enzyme was overexpressed in escherichia coli, purified and crystallized by means of the hanging-drop vapour-diffusion method with ammonium sulfate as the precipitant. the crystals were orthorhombic and belonged to space group p2(1)2(1)2(1), with unit-cell parameters a = 66.2, b = 83.7, c = 103.8 a. assuming two molecul ...200312925806
ph and deuterium kinetic isotope effects studies on the oxidation of choline to betaine-aldehyde catalyzed by choline oxidase.the fad-dependent choline oxidase catalyzes the four-electron oxidation of choline to glycine-betaine, with betaine-aldehyde as intermediate. the enzyme is capable of accepting either choline or betaine-aldehyde as a substrate, allowing the investigation of the reaction mechanism for both the conversion of choline to betaine-aldehyde and of betaine-aldehyde to glycine-betaine. in the present study, ph and deuterium kinetic isotope effects with [1,2-2h(4)]-choline were used to study the mechanism ...200312922164
cold adaptation of a psychrophilic chitinase: a mutagenesis study.the gene encoding chitinase archib from the antarctic arthrobacter sp. tad20 has been expressed in escherichia coli and the recombinant enzyme purified to homogeneity. in an effort to engineer cold-adapted biocatalysts through rational redesign to operate at elevated temperatures, we performed several mutations aiming to increase the rigidity of the molecular edifice of the selected psychrophilic chitinase. the mutations were designed on the basis of a homology-based three-dimensional model of t ...200312915727
channelling and formation of 'active' formaldehyde in dimethylglycine we report crystal structures of dimethylglycine oxidase (dmgo) from the bacterium arthrobacter globiformis, a bifunctional enzyme that catalyzes the oxidation of n,n-dimethyl glycine and the formation of 5,10-methylene tetrahydrofolate. the n-terminal region binds fad covalently and oxidizes dimethylglycine to a labile iminium intermediate. the c-terminal region binds tetrahydrofolate, comprises three domains arranged in a ring-like structure and is related to the t-protein of the glycine c ...200312912903
isolation of microorganisms for biological detoxification of lignocellulosic hydrolysates.acid pretreatment of lignocellulosic biomass releases furan and phenolic compounds, which are toxic to microorganisms used for subsequent fermentation. in this study, we isolated new microorganisms for depletion of inhibitors in lignocellulosic acid hydrolysates. a sequential enrichment strategy was used to isolate microorganisms from soil. selection was carried out in a defined mineral medium containing a mixture of ferulic acid (5 mm), 5-hydroxymethylfurfural (5-hmf, 15 mm), and furfural (20 m ...200412908085
the structure of 4-hydroxybenzoyl-coa thioesterase from arthrobacter sp. strain su.the 4-chlorobenzoyl-coa dehalogenation pathway in certain arthrobacter and pseudomonas bacterial species contains three enzymes: a ligase, a dehalogenase, and a thioesterase. here we describe the high resolution x-ray crystallographic structure of the 4-hydroxybenzoyl-coa thioesterase from arthrobacter sp. strain su. the tetrameric enzyme is a dimer of dimers with each subunit adopting the so-called "hot dog fold" composed of six strands of anti-parallel beta-sheet flanked on one side by a rathe ...200312907670
[xylose(glucose) isomerase reactivity of immobilized arthrobacter sp].the study of the xylose/glucose isomerase-containing arthrobacter sp. b-5 cells immobilized in cobalt hydroxide gel showed that immobilization increases the substrate affinity of the enzyme, its thermo- and ph-optima of action and stability, and makes unnecessary the addition of stabilizing cobalt ions to the isomerization medium.200312901016
purification and properties of a heat-stable inulin fructotransferase from arthrobacter inulin fructotransferase producing difructose dianhydride i (ec was purified from arthrobacter ureafaciens a51-1. it had maximum activity at ph 5.5 and 45 degrees c, and was stable up to 80 degrees c. this is the highest thermal stability for this enzyme reported to date. the molecular mass was estimated to be 38000 by sds-page, and 61000 by gel filtration. it was therefore estimated to be a dimer.200312889813
mode of action and antifungal properties of two cold-adapted chitinases.the mode of action of two chitinases from the antarctic arthrobacter sp. strain tad20 on n-acetyl-chitooligomers and chitin polymers has been elucidated. identification of the length of chitin oligomers following enzymatic hydrolysis was verified by using hplc-based analysis. it was observed that the length of the oligomer is important for enzyme action. the enzymes cannot effectively hydrolyze chitin oligomers with a degree of polymerization lower than four. archia is an endochitinase which hyd ...200312884086
isolation and characterization of psychrophiles producing cold-active beta-galactosidase.the present study was conducted to screen for psychrophilic micro-organisms that are able to hydrolyse lactose at low temperature, and to examine the cold-active beta-galactosidase produced by the isolated psychrophilic micro-organisms.200312859659
catabolism of arylboronic acids by arthrobacter nicotinovorans strain pba.arthrobacter sp. strain pba metabolized phenylboronic acid to phenol. the oxygen atom in phenol was shown to be derived from the atmosphere using (18)o(2). 1-naphthalene-, 2-naphthalene-, 3-cyanophenyl-, 2,5-fluorophenyl-, and 3-thiophene-boronic acids were also transformed to monooxygenated products. the oxygen atom in the product was bonded to the ring carbon atom originally bearing the boronic acid substituent with all the substrates tested.200312839810
characterization and molecular cloning of a novel enzyme, inorganic polyphosphate/atp-glucomannokinase, of arthrobacter sp. strain km.a bacterium exhibiting activities of several inorganic polyphosphate [poly(p)]- and atp-dependent kinases, including glucokinase, nad kinase, mannokinase, and fructokinase, was isolated, determined to belong to the genus arthrobacter, and designated arthrobacter sp. strain km. among the kinases, a novel enzyme responsible for the poly(p)- and atp-dependent mannokinase activities was purified 2,200-fold to homogeneity from a cell extract of the bacterium. the purified enzyme was a monomer with a ...200312839753
a novel enzyme, d-3-hydroxyaspartate aldolase from paracoccus denitrificans ifo 13301: purification, characterization, and gene cloning.a novel enzyme, d-3-hydroxyaspartate aldolase (d-haa), catalyzing the conversion of d-3-hydroxyaspartate to glyoxylate plus glycine, was purified to homogeneity from paracoccus denitrificans ifo 13301. d-haa is strictly d-specific as to the alpha-position, whereas the enzyme does not distinguish between threo and erythro forms at the beta-position. in addition to d-3-hydroxyaspartate, the enzyme also acts on d-threonine, d-3-3,4-dihydroxyphenylserine, d-3-3,4-methylenedioxyphenylserine, and d-3- ...200312835921
members of the genus arthrobacter grow anaerobically using nitrate ammonification and fermentative processes: anaerobic adaptation of aerobic bacteria abundant in soil.members of the genus arthrobacter are usually regarded as obligate aerobic bacteria. the anaerobic growth and energy metabolism of two arthrobacter species were investigated. arthrobacter globiformis utilized both nitrate ammonification and lactate, acetate and ethanol producing fermentation processes for anaerobic growth. only nitrate supported anaerobic growth of arthrobacter nicotianae. anaerobically induced respiratory nitrate reductase activity was detected in both strains. neither of the t ...200312829291
naphthalene-utilizing and mercury-resistant bacteria isolated from an acidic environment.soil samples were taken from areas of low ph (2.5-3.5) surrounding an outdoor coal storage pile. these samples were added to medium with naphthalene as the sole carbon source to enrich for organisms capable of degrading polycyclic aromatic hydrocarbons (pah) at low ph. five such bacterial strains were isolated. sequencing of the 16s rdna showed them to be members of the genera clavibacter, arthrobacter and acidocella. these organisms were all capable of growth with naphthalene as a sole carbon s ...200312827325
a calorimetric characterization of cr(vi)-reducing arthrobacter oxydans at different phases of the cell growth cycle.this is the first of a series of calorimetric studies designed to characterize and understand survival mechanisms of metal-reducing bacteria isolated from metal-polluted environments. in this paper we introduce a new concept of thermal spectrum of the endothermic melting of complex biological systems (e.g., proteins, nucleic acids, ribosomes, membrane structures) in intact cells. all thermal spectra measured are thermograms that describe the temperature dependence of heat capacity change of the ...200312806104
gold electrodes wired for coupling with the deeply buried active site of arthrobacter globiformis amine oxidase.diethylaniline-terminated oligo(phenyl-ethynyl)-thiol (dea-ope-sh) wires on au-bead electrodes facilitate electron tunneling to and from the deeply buried topaquinone (tpq) cofactor in arthrobacter globiformis amine oxidase (agao). reversible cyclic voltammograms were observed when agao was adsorbed onto this dea-ope-sau surface: the 2e-/2h+ reduction potential is -140 mv versus sce.200312797771
structural characterization and mapping of the covalently linked fad cofactor in choline oxidase from arthrobacter globiformis.the flavoenzyme choline oxidase catalyzes the oxidation of choline and betaine aldehyde to betaine. earlier studies have shown that the choline oxidase from arthrobacter globiformis contains fad covalently linked to a histidine residue. to identify the exact type of flavin binding, the fad-carrying amino acid residue was released by acid hydrolysis. the fluorescence excitation maxima of the isolated aminoacylriboflavin, showing a hypsochromic shift of the near-ultraviolet band relative to ribofl ...200312795615
toxicity of hexavalent chromium and its reduction by bacteria isolated from soil contaminated with tannery arthrobacter sp. and a bacillus sp., isolated from a long-term tannery waste contaminated soil, were examined for their tolerance to hexavalent chromium [cr(vi)] and their ability to reduce cr(vi) to cr(iii), a detoxification process in cell suspensions and cell extracts. both bacteria tolerated cr(vi) at 100 mg/ml on a minimal salts agar medium supplemented with 0.5% glucose, but only arthrobacter could grow in liquid medium at this concentration. arthrobacter sp. could reduce cr(vi) up to 5 ...200312783193
ahld, an n-acylhomoserine lactonase in arthrobacter sp., and predicted homologues in other bacteria.quorum sensing is a signalling mechanism that controls diverse biological functions, including virulence, via n-acylhomoserine lactone (ahl) signal molecules in gram-negative bacteria. with the aim of isolating strains or enzymes capable of blocking quorum sensing by inactivating ahl, bacteria were screened for ahl degradation by their ability to utilize n-3-oxohexanoyl-l-homoserine lactone (ohhl) as the sole carbon source. among four isolates, strain ibn110, identified as arthrobacter sp., was ...200312777494
a set of glycoproteins recognized by a2b5 antibody with major bands at 55 and 76 kda is overexpressed in human head and neck squamous cell carcinomas.a2b5 antibody was found to strongly label frozen sections of human head and neck squamous cell carcinomas. the low amount of glycolipids (c-series gangliosides and sulfatides) purified from the same tumors and reactive with a2b5 by immunostaining on thin-layer plates could not account for the high level of tissue labeling. proteins were extracted from both normal tissues and squamous cell carcinomas and analyzed by western blot with a2b5 antibody on pvdf membranes. the antibody was found to stai ...200312770779
d-arginase of arthrobacter sp. kuj 8602: characterization and its identity with zn(2+)-guanidinobutyrase.d-arginase activity was found in the cells of an isolate, arthrobacter sp. kuj 8602, grown in the l-arginine medium, and the enzyme was purified and characterized. its molecular weight was estimated to be about 232,000 by gel filtration, and that of the subunit was approximately 40,000 by sds-page, suggesting that the enzyme is a homohexamer. the enzyme acted on not only d-arginine but also 4-guanidinobutyrate, 3-guanidinopropionate and even l-arginine. the v(max)/k(m) values for 4-guanidinobuty ...200312761196
[the effect of sulfur on the hydrocarbon-oxidizing bacteria of different genera growth]. 200312751253
characterization of the 4-hydroxybenzoyl-coenzyme a thioesterase from arthrobacter sp. strain su.the arthrobacter sp. strain su 4-chlorobenzoate (4-cba) dehalogenation pathway converts 4-cba to 4-hydroxybenzoate (4-hba). the pathway operon contains the genes fcba, fcbb, and fcbc (a. schmitz, k. h. gartemann, j. fiedler, e. grund, and r. eichenlaub, appl. environ. microbiol. 58:4068-4071, 1992). genes fcba and fcbb encode 4-cba-coenzyme a (coa) ligase and 4-cba-coa dehalogenase, respectively, whereas the function of fcbc is not known. we subcloned fcbc and expressed it in escherichia coli, a ...200312732540
gene cluster of arthrobacter ilicis ru61a involved in the degradation of quinaldine to anthranilate: characterization and functional expression of the quinaldine 4-oxidase qoxlms genes.a genetic analysis of the anthranilate pathway of quinaldine degradation was performed. a 23-kb region of dna from arthrobacter ilicis rü61a was cloned into the cosmid pvk100. although escherichia coli clones containing the recombinant cosmid did not transform quinaldine, cosmids harboring the 23-kb region, or a 10.8-kb stretch of this region, conferred to pseudomonas putida kt2440 the ability to cometabolically convert quinaldine to anthranilate. the 10.8-kb fragment thus contains the genes cod ...200312730200
metal resistance among aerobic chemoheterotrophic bacteria from the deep terrestrial subsurface.the metal resistance of 350 subsurface bacterial strains from two u.s. department of energy facilities, the savannah river site (srs), south carolina, and the hanford site, washington, was determined to assess the effect of metal toxicity on microorganisms in the deep terrestrial subsurface. resistance was measured by growth inhibition around discs containing optimized amounts of hg(ii), pb(ii), and cr(vi). a broad range of resistance levels was observed, with some strains of arthrobacter spp. d ...200312718404
[the 1(2)-dehydrogenation of steroid substrates by nocardioides simplex vkm ac-2033d].the bacterium formerly known as arthrobacter globiformis 193 has high 1(2)-dehydrogenase activity toward pharmaceutically important steroids, 9(11)-dehydrocortexolone in particular. the complex analysis of the morphostructural, physiological, biochemical, and phylogenetic properties of this bacterium allowed us to reclassify it into nocardioides simplex (n. simplex vkm ac-2033d).200312698789
levan fructotransferase from arthrobacter oxydans j17-21 catalyzes the formation of the di-d-fructose dianhydride iv from levan.a new levan fructotransferase (lftase) isolated from arthrobacter oxydans j17-21 was characterized for the production of difructose dianhydride iv (dfa iv). lftase was purified to apparent homogeneity by q-sepharose ion exchange chromatography, mono-q hr 5/5 column chromatography, and gel permeation chromatography. the enzyme had an apparent molecular mass of 54000 da. the optimum ph for the enzyme-catalyzed reaction was ph 6.5, and the optimum temperature was observed at 45 degrees c. the lftas ...200312696949
process for producing the potential food ingredient dfa iii from inulin: screening, genetic engineering, fermentation and immobilisation of inulase ii.difructose anhydride (dfa iii) is a new potential sweet food additive. a screening was undertaken to isolate bacterial strains for conversion of inulin to dfa. of special interest were thermotolerant enzymes. some 400 strains were investigated, among four of them produce dfa and strain buo141 expresses an extracellular enzyme which is stable at elevated temperatures. based on metabolic data and 16s-rrna-sequencing, the strain was identified as a new arthrobacter species. for increased enzyme pro ...200312695027
inhibition of six copper-containing amine oxidases by the antidepressant drug tranylcypromine.potential inhibitory effects of the clinically utilized monoamine oxidase inhibitor tranylcypromine (tcp) on mammalian, plant, bacterial, and fungal copper-containing amine oxidases have been examined. the following enzymes have been investigated: human kidney diamine oxidase (hkao), bovine plasma amine oxidase (bpao), equine plasma amine oxidase (epao), pea seedling amine oxidase (psao), arthrobacter globiformis amine oxidase (agao), and pichia pastoris lysyl oxidase (pplo). only bpao, epao, an ...200312686142
isolation and characterization of an atrazine-degrading bacterium from industrial wastewater in isolate and characterize atrazine-degrading bacteria in order to identify suitable candidates for potential use in bioremediation of atrazine contamination.200312680937
agar-plated bacteria found in the activated sludge of lab-scale sbr and cfstr systems.we identified and compared the predominant agar-plated bacteria in the activated sludge of two popular wastewater treatment systems, the sequencing batch reactor (sbr) and the continuous-flow stirred tank reactor (cfstr). all tests had the same feed composition except for the buffer intensity. it was found that corynebacterium sp. seemed to prefer growing under lower buffer condition, and arthrobacter sp. grew dominantly in the system with higher buffer intensity. gram-negative bacteria appeared ...200312676503
dimethylphthalate hydrolysis by specific microbial esterase.two bacterial strains: arthrobacter sp. and sphingomonas paucimobilis were isolated from soil by enrichment cultures using dimethylphthalate (dmp) or monomethylphthalate (mmp) as sole carbon source, respectively. dmp was rapidly transformed by an arthrobacter sp. culture with formation of mmp and phthalic acid (pa) which is further degraded. this strain was unable to hydrolyse mmp. a mechanism of degradation of dmp was proposed with two ways: dmp-->pa and dmp-->mmp. the s. paucimobilis strain hy ...200312668024
[the existence of contamination in enzymes used for the isolation of aspergillus and candida species dna].molecular biological methods which are widely used in different fields, have been replaced with conventional diagnostic tests for the early diagnosis of invasive fungal infections, recently. polymerase chain reaction (pcr) is one of these methods, with high specificity and sensitivity, which is accepted throughout the world. however, the enzymes that are used for the isolation of target dna, may be contaminated with the gene sequences of some other fungal species in the preparation steps and may ...200212652873
proteolytic activity of a yeast cell wall lytic arthrobacter investigate properties of the proteolytic activity of a yeast cell wall lytic soil bacterium identified as an arthrobacter species.200312641716
kinetic mechanism of choline oxidase from arthrobacter globiformis.choline oxidase catalyzes the four-electron oxidation of choline to glycine-betaine, with betaine-aldehyde as intermediate and molecular oxygen as primary electron acceptor. the enzyme is capable of accepting betaine-aldehyde as a substrate, allowing the investigation of the reaction mechanism for both the conversion of choline to the aldehyde intermediate and of betaine-aldehyde to glycine-betaine. the steady state kinetic mechanism has been determined at ph 7 with choline and betaine-aldehyde ...200312637017
predominant bacillus spp. in agricultural soil under different management regimes detected via pcr-dgge.a pcr system for studying the diversity of species of bacillus and related taxa directly from soil was developed. for this purpose, a specific 24-bp forward primer located around position 110 of the 16s ribosomal rna gene was designed and combined with a reverse bacterial primer located at the end of the gene. the specificity of this pcr system for bacilli and related taxons was confirmed on the basis of tests with diverse strains as well as with soil dna. analysis of a soil dna derived clone li ...200312632212
respiration of 13c-labeled substrates added to soil in the field and subsequent 16s rrna gene analysis of 13c-labeled soil dna.our goal was to develop a field soil biodegradation assay using (13)c-labeled compounds and identify the active microorganisms by analyzing 16s rrna genes in soil-derived (13)c-labeled dna. our biodegradation approach sought to minimize microbiological artifacts caused by physical and/or nutritional disturbance of soil associated with sampling and laboratory incubation. the new field-based assay involved the release of (13)c-labeled compounds (glucose, phenol, caffeine, and naphthalene) to soil ...200312620850
sequence of the 165-kilobase catabolic plasmid pao1 from arthrobacter nicotinovorans and identification of a pao1-dependent nicotine uptake system.the 165-kb catabolic plasmid pao1 enables the gram-positive soil bacterium arthrobacter nicotinovorans to grow on the tobacco alkaloid l-nicotine. the 165,137-nucleotide sequence, with an overall g+c content of 59.7%, revealed, besides genes and open reading frames (orfs) for nicotine degradation, a complete set of orfs for enzymes essential for the biosynthesis of the molybdenum dinucleotide cofactor, as well as orfs related to uptake and utilization of carbohydrates, sarcosine, and amino acids ...200312618462
4-nitrocatechol as a probe of a mn(ii)-dependent extradiol-cleaving catechol dioxygenase (mndd): comparison with relevant fe(ii) and mn(ii) model 3,4-dihydroxyphenylacetate 2,3-dioxygenase (mndd) is an extradiol-cleaving catechol dioxygenase from arthrobacter globiformis that has 82% sequence identity to and cleaves the same substrate (3,4-dihydroxyphenylacetic acid) as fe(ii)-dependent 3,4-dihydroxyphenylacetate 2,3-dioxygenase (hpcd) from brevibacterium fuscum. we have observed that mndd binds the chromophoric 4-nitrocatechol (4-nch(2)) substrate as a dianion and cleaves it extremely slowly, in contrast to the fe(ii)-de ...200312589562
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