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use of the mushroom tissue block rapid pitting test to detect brown blotch pathogens.isolates of an arthrobacter species known to produce a positive host pathogenicity test were evaluated by the mushroom tissue block rapid pitting test. all 11 isolates of the arthrobacter species yielded a positive test response. the time required for a positive test was the same for pseudomonas tolaasi and the arthrobacter species.198516346775
production of d-tagatose from dulcitol by arthrobacter globiformis.a process for the bacterial oxidation of dulcitol to d-tagatose has been developed. the strain arthrobacter globiformis st48 used in this fermentation was isolated from soil. the yield of d-tagatose accumulated in the medium from dulcitol was as high as 85%. about 14 g of d-tagatose crystals was isolated from 1 liter of 2% dulcitol medium.198416346663
degradation of 4-chlorobenzoic acid by arthrobacter sp.a mixed population, enriched and established in a defined medium, from a sewage sludge inoculum was capable of complete mineralization of 4-chlorobenzoate. an organism, identified as arthrobacter sp., was isolated from the consortium and shown to be capable of utilizing 4-chlorobenzoate as the sole carbon and energy source in pure culture. this organism (strain tm-1), dehalogenated 4-chlorobenzoate as the initial step in the degradative pathway. the product, 4-hydroxybenzoate, was further metabo ...198416346660
distribution of brown blotch bacteria in wild and cultivated species of basidiomycetes.wild and cultivated basidiomycetes species were cultured to determine the distribution of bacteria causing brown blotch disease of agaricus bisporus. colonies from each basidiocarp were screened for brown blotch organisms by the white line and host pathogenicity tests. isolates causing brown blotch were identified as pseudomonas tolaasi and an arthrobacter species.198416346653
formation and identification of interfacial-active glycolipids from resting microbial cells.resting cells of arthrobacter sp. strain dsm2567 incubated in the presence of various mono-, di-, or trisaccharides biosynthesized different glycolipids. all eight glycolipids, containing the corresponding carbohydrate moiety and one, two, or three alpha-branched beta-hydroxy fatty acids, were produced when mannose, glucose, cellobiose, maltose, and maltotriose were used as carbon sources in a simple phosphate buffer. the structures of the compounds were elucidated by means of h and c nuclear ma ...198416346628
isolation of a bacterium capable of degrading peanut hull lignin.thirty-seven bacterial strains capable of degrading peanut hull lignin were isolated by using four types of lignin preparations and hot-water-extracted peanut hulls. one of the isolates, tentatively identified as arthrobacter sp., was capable of utilizing all four lignin preparations as well as extracted peanut hulls as a sole source of carbon. the bacterium was also capable of degrading specifically labeled [c]lignin-labeled lignocellulose and [c]cellulose-labeled lignocellulose from the cordgr ...198316346424
bacterial methylation of chlorinated phenols and guaiacols: formation of veratroles from guaiacols and high-molecular-weight chlorinated lignin.two strains of bacteria, provisionally assigned to the genus arthrobacter, were shown to metabolize mono-, di-, tri-, and tetrachloroguaiacols and pentachlorophenol to the corresponding o-methyl compounds. hydroxylated intermediates were formed only transiently, except for the synthesis by one strain of 3,4,5-trichlorosyringol from 3,4,5-trichloroguaiacol. two isomeric trichloroveratroles and tetrachloroveratrole were formed by three of the strains from a high-molecular-weight chlorinated lignin ...198316346242
microbial treatment of soil to remove pentachlorophenol.direct inoculation of bacteria capable of degrading pentachlorophenol (pcp) into pcp-contaminated soil was investigated as a prophylactic measure to reduce the hazards of runoffs when spills occur or when wooden poles freshly treated with pcp-containing preservatives are located near streams and lakes. in laboratory tests at 30 degrees c, the direct addition of 10 pcp-utilizing arthrobacter cells per g of dry soil reduced the half-life of the pesticide from 2 weeks to <1 day. soil inoculation al ...198316346232
possible role of coryneform bacteria in age gelation of ultrahigh-temperature-processed milk.two strains of psychrotrophic, gram-positive, proteolytic bacteria isolated from raw milk were identified as arthrobacter or closely related coryneform species. we inoculated raw-milk samples with the strains (one strain per sample) and compared rates of gelation after ultrahigh-temperature processing with that of ultrahigh-temperature-processed controls. the trial indicated that either organism could play a role in age gelation of ultrahigh-temperature-processed milk.198216346043
isolation of arthrobacter bacteriophage from soil.soil was percolated with water and various nutrient solutions, and then the percolates were analyzed for bacteriophages which produced plaques on various arthrobacter strains. the water percolates did not contain detectable phage. in contrast, phages for a. globiformis strains atcc 8010 and 4336, and for several recent arthrobacter species soil isolates, were easily detected in nutrient broth, soil extract, and cation-complete medium percolates. these percolates did not contain phage that produc ...198116345792
elution and inactivation of bacteriophages on soil and cation-exchange resin.a variety of elution schemes was tested to determine the most effective procedure for eluting arthrobacter bacteriophages from soil. a buffer solution of ph 8.0 was found to be the most satisfactory eluent. bacteriophages were adsorbed to cation-exchange sites on soils, clays, and dowex-50 resin and eluted. eluted bacteriophages were detected by passive hemagglutination and plaque assay. although bacteriophage antigen was successfully eluted, most recovered bacteriophages were noninfective. inac ...197916345416
attachment to autoclaved soil of bacterial cells from pure cultures of soil isolates.pure cultures of arthrobacter globiformis and four fresh soil isolates were incubated individually in autoclaved soil, in both the presence and absence of glucose. these bacteria grew in the soil and, except for a. globiformis, eventually attached firmly to the soil solids. firmly attached cells were defined as those which could not be separated from the soil solids by blending combined with a series of low-speed centrifugal washings. the attachment attained by the soil isolates appeared to dupl ...197916345373
metabolism of di- and mono-n-butyl phthalate by soil bacteria.di-n-butyl phthalate and other dialkyl phthalates are used as carbon sources by three nocardia sp. isolates; mono-n-butyl phthalate is used as a carbon source by an arthrobacter sp. isolate and a pseudomonas sp. isolate. the compounds were metabolized in these organisms by hydrolysis to the corresponding monoesters and free phthalic acid. phthalic acid was then metabolized via protocatechuic acid by 3,4-dioxygenative ring cleavage.197816345266
extradiol cleavage of 3-methylcatechol by catechol 1,2-dioxygenase from various microorganisms.the isofunctional enzymes of catechol 1,2-dioxygenase from species of acinetobacter, pseudomonas, nocardia, alcaligenes, and corynebacterium oxidize 3-methylcatechol according to both the intradiol and extradiol cleavage patterns. however, the enzyme preparations from brevibacterium and arthrobacter have only the intradiol cleavage activity. comparison of substrate specificity among these isofunctional dioxygenases shows striking differences in the oxidation of 3-methylcatechol, 4-methylcatechol ...197716345232
chemo-enzymatic synthesis of eel calcitonin glycosylated at two sites with the same and different carbohydrate structures.naturally occurring glycopeptides and glycoproteins usually contain more than one glycosylation site, and the structure of the carbohydrate attached is often different from site to site. therefore, synthetic methods for preparing peptides and proteins that are glycosylated at multiple sites, possibly with different carbohydrate structures, are needed. here, we report a chemo-enzymatic approach for accomplishing this. complex-type oligosaccharides were introduced to the calcitonin derivatives tha ...200616343462
utilization of homoserine lactone as a sole source of carbon and energy by soil arthrobacter and burkholderia species.homoserine lactone (hsl) is a ubiquitous product of metabolism. it is generated by all known biota during the editing of certain mischarged aminoacyl-trna reactions, and is also released as a product of quorum signal degradation by bacterial species expressing acyl-hsl acylases. little is known about its environmental fate over long or short periods of time. the mammalian enzyme paraoxonase, which has no known homologs in bacteria, has been reported to degrade hsl via a lactonase mechanism. cert ...200616341844
microbiology and biochemistry of nicotine degradation.several bacterial species are adapted to nicotine, the main alkaloid produced by the tobacco plant, as growth substrate. a general outline of nicotine catabolism by these bacteria is presented, followed by an emphasis on new insights based on molecular biology and biochemical work obtained with the catabolic plasmid pao1 of arthrobacter nicotinovorans. its 165-kb sequence revealed the genetic structure of nicotine catabolism and allowed the assignment of new enzyme activities to specific gene pr ...200616333621
plasmids for nicotine-dependent and -independent gene expression in arthrobacter nicotinovorans and other arthrobacter species.the first inducible arthrobacter overexpression system, based on the promoter/operator and the repressor of the 6-d-hydroxynicotine oxidase gene of arthrobacter nicotinovorans, is described here. nicotine-dependent overproduction and affinity purification of recombinant proteins are presented. the system will allow the production of complex enzymes and genetic complementation studies in arthrobacter species.200516332890
class 1 integrons and tetracycline resistance genes in alcaligenes, arthrobacter, and pseudomonas spp. isolated from pigsties and manured soil.the presence of tetracycline resistance (tc(r)) genes and class i integrons (in-1), and their ability to cotransfer were investigated in tc(r) gram-negative (185 strains) and gram-positive (72 strains) bacteria from danish farmland and pigsties. the isolates belonged to the groups or species escherichia coli, enterobacter spp., arthrobacter spp., alcaligenes spp., pseudomonas spp., and corynebacterium glutamicum. the 257 isolates were screened for in-1. eighty-one of the gram-negative isolates w ...200516332771
epiphytic microorganisms on strawberry plants (fragaria ananassa cv. elsanta): identification of bacterial isolates and analysis of their interaction with leaf surfaces.epiphytic bacteria were isolated from strawberry plants cultivated in the field or in the greenhouse in order to investigate their interaction with leaf-surface transport properties. colonization of lower leaf sides was higher on field-grown plants, whereas upper leaf sides were more densely colonized on plants cultivated in the greenhouse. fungal isolates significantly contributed to total microbial biomass on leaf surfaces of greenhouse-grown strawberry plants, whereas these organisms were rar ...200516329966
impacts on microbial communities and cultivable isolates from groundwater contaminated with high levels of nitric acid-uranium waste.microbial communities were characterized at contaminated sites that had elevated levels of nitrate, nickel, aluminum, and uranium (up to 690 mm, 310 microm, 42 mm, and 30 microm, respectively). the bacterial community structure based upon clonal libraries of the ssu rrna genes (screened clones = 876) was diverse at the background site, but the three acidic samples had decreased diversity and the majority of clones were closely related to azoarcus and pseudomonas species. arthrobacter and novosph ...200516329960
characterization of arthrobacter nicotinovorans him, an atrazine-degrading bacterium, from agricultural soil new zealand.arthrobacter nicotinovorans him was isolated directly from an agricultural sandy dune soil 6 months after a single application of atrazine. it grew in minimal medium with atrazine as sole nitrogen source but was unable to mineralize 14c-ring-labelled atrazine. atrazine was degraded to cyanuric acid. in addition to atrazine the bacterium degraded simazine, terbuthylazine, propazine, cyanazine and prometryn but was unable to grow on terbumeton. when added to soil, a. nicotinovorans him did enhance ...200416329913
characterization of microbial contamination in united states air force aviation fuel tanks.bacteria and fungi, isolated from united states air force (usaf) aviation fuel samples, were identified by gas chromatograph fatty acid methyl ester (gc-fame) profiling and 16s or 18s rrna gene sequencing. thirty-six samples from 11 geographically separated usaf bases were collected. at each base, an above-ground storage tank, a refueling truck, and an aircraft wing tank were sampled at the lowest sample point, or sump, to investigate microbial diversity and dispersion within the fuel distributi ...200616328508
an alpha/beta-fold c--c bond hydrolase is involved in a central step of nicotine catabolism by arthrobacter nicotinovorans.the enzyme catalyzing the hydrolytic cleavage of 2,6-dihydroxypseudooxynicotine to 2,6-dihydroxypyridine and gamma-n-methylaminobutyrate was found to be encoded on pao1 of arthrobacter nicotinovorans. the new enzyme answers an old question about nicotine catabolism and may be the first c--c bond hydrolase that is involved in the biodegradation of a heterocyclic compound.200516321959
[characterization of communities of heterotrophic bacteria associated with healthy and diseased corals in nha trang bay (vietnam)].a comparative investigation of the heterotrophic microflora of 11 species of healthy corals and of white-band-diseased and yellow-band-diseased corals inhabiting the reefs of nha trang bay (vietnam), which has been exposed to anthropogenic impact, was performed. fifty-nine strains of heterotrophic bacteria isolated on y/k and endo media were investigated and characterized. all the isolates were identified at the genus level by consideration of the results of analysis of their phenotypic properti ...200516315986
degradation of di-butyl-phthalate by soil bacteria.twelve gram-positive phthalate ester degraders were isolated from soil. using biolog gp2 plates, eight of them were identified as belonging to the corynebacterium-mycobacterium-nocardia group, while the remaining four were unidentifiable. when cultured in the presence of di-butyl-phthalate (dbp) in basal salts solution, five of these isolates accomplished more than 90% of dbp degradation within 48 h (fast group), three were placed in the medium group, and the remaining four were placed in the sl ...200616289698
development of a microtiter plate-based method for determination of degradation profile of nitrophenolic compounds.a microtiter plate-based assay was developed for the automatic monitoring of degradation profile of the yellow-coloured nitrophenolic compounds. the method enables to reduce the intervals between measurements of substrate concentration to minutes and to overcome the problem of discontinuity of sampling typical for conventional methods. the concentrations of nitrophenolic compounds were calculated from the absorbance values determined automatically by bioscreen c. verification of the method was b ...200616289394
[the product identification study on the isolating process of strains produced trehalose].in the isolating process of stains whose endocellular enzymes can produce trehalose on starch or maltooligosaccharides, we discovered the components of enzymatic reactant were complicated and it was difficult to purify them each other, however, we have to know quickly whether there was tehalose in the enzymatic reactant above. in order to make it clearly, thinner layer chromatography, high performance liquid chromatography-electrospray ionization mass spectrometry and 13c-nuclear magnetic resona ...200316276879
novel 4-chlorophenol degradation gene cluster and degradation route via hydroxyquinol in arthrobacter chlorophenolicus a6.arthrobacter chlorophenolicus a6, a previously described 4-chlorophenol-degrading strain, was found to degrade 4-chlorophenol via hydroxyquinol, which is a novel route for aerobic microbial degradation of this compound. in addition, 10 open reading frames exhibiting sequence similarity to genes encoding enzymes involved in chlorophenol degradation were cloned and designated part of a chlorophenol degradation gene cluster (cph genes). several of the open reading frames appeared to encode enzymes ...200516269679
surface microflora of four smear-ripened cheeses.the microbial composition of smear-ripened cheeses is not very clear. a total of 194 bacterial isolates and 187 yeast isolates from the surfaces of four irish farmhouse smear-ripened cheeses were identified at the midpoint of ripening using pulsed-field gel electrophoresis (pfge), repetitive sequence-based pcr, and 16s rrna gene sequencing for identifying and typing the bacteria and fourier transform infrared spectroscopy and mitochondrial dna restriction fragment length polymorphism (mtdna rflp ...200516269673
[patterns of formation of microbial communities of alluvial soils in the selenga estuary].microbial communities of alluvial soils in the facies on islands in the selenga estuary and near stepnoi dvorets and istomino settlements were studied. the distribution of the cfu-dominant microorganisms (bacillus and pseudomonas + arthrobacter) has been revealed in the studied island soils. in addition, actinomycetes streptomyces were found among the dominants in soils of the near-terrace floodplain. the quantitative distribution of microorganisms across soil layers was demonstrated. the effect ...200516240750
hypothesis: structures, evolution, and ancestor of glucose kinases in the hexokinase family.glucose kinase, which we tentatively use in this review, represents the enzymes catalyzing the phosphorylation of glucose and other hexoses by means of phosphoryl donors (atp, adp, and inorganic polyphosphate [poly(p)]). except for glucose kinases utilizing adp, all other glucose kinases belong to the hexokinase (hk) family and are classified into three groups based on primary structural information, i.e., groups hk, a, and b. the structural and evolutionary relationships of glucose kinases belo ...200516233797
molecular cloning and expression of uricase gene from arthrobacter globiformis in escherichia coli and characterization of the gene product.arthrobacter globiformis ferm bp-360 produces uricase (urate oxidase; ec 1.7.3.3) intracellularly. a genomic library of the bacterium, prepared in the plasmid vector puc118, was screened with probes based on the amino acid sequence of the purified uricase. we found that a chimeric plasmid in the library, designated puod1, carries a 2.0-kb dna insert from the arthrobacter dna that hybridizes with the probe. the dna insert contains an orf consisting of 302 amino acids with a calculated molecular m ...200416233683
cloning and heterologous expression of a beta-fructofuranosidase gene from arthrobacter globiformis ifo 3062, and site-directed mutagenesis of the essential aspartic acid and glutamic acid of the active site.we have cloned the gene encoding a beta-fructofuranosidase from arthrobacter globiformis ifo 3062, and subsequently, the gene was heterologously expressed in escherichia coli. this beta-fructofuranosidase gene encodes a protein of 548 amino acid residues with a calculated molecular mass of 60,519 da. we have examined the roles of three residues of a. globiformis ifo 3062 beta-fructofuranosidase by site-directed mutagenesis, and found that aspartic acid 130 and glutamic acid 392, which are two of ...200416233623
cloning and heterologous expression of a glucodextranase gene from arthrobacter globiformis i42, and experimental evidence for the catalytic diad of the recombinant enzyme.the gene encoding a glucodextranase from arthrobacter globiformis i42 was cloned and, subsequently, heterologously expressed in escherichia coli. this glucodextranase gene consists of 1048 amino acid residues with a calculated molecular mass of 109,135 da. the roles of two residues at the active site of a. globiformis i42 glucodextranase were examined by site-directed mutagenesis. glutamic acid residues 458 and 656, which are part of the apparent catalytic residues, were found to be essential fo ...200416233603
a thermostable histamine oxidase from arthrobacter crystallopoietes kait-b-007.a thermostable histamine oxidase (ec 1.4.3.-) was found in cells of arthrobacter crystallopoietes kait-b-007 isolated from soil. the enzyme was purified about 715-fold over the cell free extracts with a yield of 55% by ammonium sulfate fractionation and various column chromatographies. the purified enzyme was homogeneous on polyacrylamide gel-electrophoresis (native-page). when the enzyme was kept at 65 degrees c and 70 degrees c for 10 min, the activity was fully stable at 65 degrees c and decr ...200416233600
molecular cloning of the gene encoding the di-d-fructofuranose 1,2':2,3' dianhydride hydrolysis enzyme (dfa iiiase) from arthrobacter sp. h65-7.the gene encoding an intracellular enzyme hydrolyzing di-d-fructofuranose 1,2':2,3' dianhydride (dfa iii) (dfa iiiase) was cloned from the genomic dna of arthrobacter sp. h65-7 for the first time. the single open reading frame (orf) of the dfa iiiase gene consisted of 1368-bp encoding 455 amino acids. dfa iiiase showed a phylogenetically distinct position from other inulin-degrading enzymes and showed similarity only with inulin fructotransferases (depolymerizing) (inulase ii, ec 2.4.1.93) from ...200316233453
removal and recovery of uranyl ion using various microorganisms.the adsorption of uranyl ion by microorganisms was examined. among the 76 strains of 69 species tested (23 bacteria, 20 actinomycetes, 18 fungi, and 15 yeasts), high uranyl ion adsorption ability was exhibited by strains of the bacteria, arthrobacter nicotianae, bacillus subtilis, and micrococcus luteus. a. nicotianae cells, which showed the best performance, could adsorb about 698 mg uranyl ion (2.58 mmol) per gram dry wt. of microbial cells. the adsorption of uranyl ion was rapid, selective, a ...200216233264
transfer of high-mannose-type oligosaccharides to disaccharides by endo-beta-n-acetylglucosaminidase from arthrobacter protophormiae.endo-beta-n-acetylglucosaminidase from arthrobacter protophormiae (endo-a) has transglycosylation activity, and high-mannose-type oligosaccharides are transferred to suitable glycosides as acceptor substrates. the acceptor specificity of endo-a-catalyzed transglycosylation toward various disaccharides was investigated. to identify an effective acceptor for the transglycosylation by endo-a, the reaction was carried out using various disaccharides. endo-a transferred high-mannose-type oligosacchar ...200216233259
purification and properties of betaine aldehyde dehydrogenase with high affinity for nadp from arthrobacter globiformis.betaine aldehyde dehydrogenase from arthrobacter globiformis was purified to apparent homogeneity by ammonium sulfate fractionation, followed by ion-exchange, butyl-toyopearl and gel filtration chromatography. the enzyme was found to be a tetramer with identical 55 kda subunits. both nad+ and nadp+ could be used as a cofactor for the enzyme and michaelis constants (k(m) value) for nad+ and nadp+ were 1075 microm and 48 microm, respectively. the enzyme was highly specific for betaine aldehyde and ...200216233177
plate assay for endo-beta-n-acetylglucosaminidase activity using a chromogenic substrate synthesized by transglycosylation with arthrobacter endo-beta-n-acetylglucosaminidase.the transglycosylation activity of arthrobacter endo-beta-n-acetylglucosaminidase (endo-a) was used for the enzymatic synthesis of a novel oligosaccharide, man6glcnac-5-bromo-4-chloro-3-indolyl-beta-glucoside (man6glcnac-glc-beta-x). various endo-beta-n-acetylglucosaminidases hydrolyzed this oligosaccharide, producing man6glcnac and glc-beta-x. the e. coli strains coexpressing endo-a and beta-glucosidase formed blue colonies in the presence of man6glcnac-glc-beta-x. therefore, endo-beta-n-acetyl ...200016232892
cloning of inulin fructotransferase (dfa iii-producing) gene from arthrobacter globiformis c11-1.a gene encoding an inulin fructotransferase (dfa iii-producing) [ec 2.4.1.93] from arthrobacter globiformis c11-1 was cloned and the nucleotide sequence was determined. the cloned fragment contained a 1353 bp open reading frame. the initiation codon was estimated to be an unusual codon, gtg. the gene encoded a signal peptide (40 amino acid residues) for secretion. the molecular mass of the native enzyme was calculated as 43,400 da from the sequencing data. the deduced amino acid sequence of the ...200016232803
isolation and characterization of psychrotrophs from subterranean environments.subterranean environments are potential sources for the isolation of novel microorganisms. water and soil samples were collected at depths ranging from 10 to 1800 meters below the surface, and screening was carried out with aerobic rich and anaerobic minimal media. two psychrotrophic and three chemoautotrophic strains were isolated. one of the psychrotrophic isolates, designated sn16a, grew at temperatures between -5 and 37 degrees c with optimal growth between 25 and 30 degrees c. the other psy ...199916232548
molecular cloning and nucleotide sequencing of organophosphorus insecticide hydrolase gene from arthrobacter sp. strain b-5.the organophosphorus insecticide hydrolase (oph) gene of arthrobacter sp. strain b-5, isolated from turf green soil was cloned into escherichia coli jm109. three clones, termed epb511, epb521 and epb531, exhibiting oph activity were obtained. however, these three clones showed lower op-degrading ability than strain b-5. a 7.7-kb inserted fragment of the plasmid pb521 harbored by epb521 was subcloned, resulting in construction of a plasmid, pb526, carrying the 2.6-kb inserted fragment with op-deg ...199916232510
chemo-enzymatic synthesis of a calcitonin derivative containing a high-mannose type oligosaccharide by endo-beta-n-acetylglucosaminidase from arthrobacter protophormiae.chemo-enzymatic addition of a high-mannose type oligosaccharide to eel calcitonin (ct), a calcium-regulating hormone, was examined. the endo-beta-n-acetylglucosaminidase from arthrobacter protophormiae (endo-a) transglycosylated the man(6)-glcnac moiety from an ovalbumin-derived high-mannose type glycosyl asparagine, asn(man(6)-glcnac(2))-oh, to a calcitonin derivative, [asn(glcnac)(3)]-ct, in which the n-acetyl-d-glucosamine (glcnac) is attached to the third l-asparagine (asn) residue of the pe ...199916232446
the role of histidine 200 in mndd, the mn(ii)-dependent 3,4-dihydroxyphenylacetate 2,3-dioxygenase from arthrobacter globiformis cm-2, a site-directed mutagenesis study.the manganese-dependent 3,4-dihydroxyphenylacetate 2,3-dioxygenase (mndd) from arthrobacter globiformis cm-2 is an extradiol-cleaving catechol dioxygenase that catalyzes aromatic ring cleavage of 3,4-dihydroxyphenylacetate (dhpa). based on the recent crystal structure of the mndd-dhpa complex, a series of site-directed mutations were made at a conserved second-sphere residue, histidine 200, to gain insight into and clarify the role this residue plays in the mn(ii)-dependent catalytic mechanism. ...200516217642
monitoring arthrobacter protophormiae rkj100 in a 'tag and chase' method during p-nitrophenol bio-remediation in soil microcosms.monitoring of micro-organisms released deliberately into the environment is essential to assess their movement during the bio-remediation process. during the last few years, dna-based genetic methods have emerged as the preferred method for such monitoring; however, their use is restricted in cases where organisms used for bio-remediation are not well characterized or where the public domain databases do not provide sufficient information regarding their sequence. for monitoring of such micro-or ...200616205921
cold-active beta-galactosidase from arthrobacter sp. c2-2 forms compact 660 kda hexamers: crystal structure at 1.9a resolution.the x-ray structure of cold-active beta-galactosidase (isoenzyme c-2-2-1) from an antarctic bacterium arthrobacter sp. c2-2 was solved at 1.9a resolution. the enzyme forms 660 kda hexamers with active sites opened to the central cavity of the hexamer and connected by eight channels with exterior solvent. to our best knowledge, this is the first cold-active beta-galactosidase with known structure and also the first known beta-galactosidase structure in the form of compact hexamers. the hexamer or ...200516171818
microbial production of optically active beta-phenylalanine ethyl ester through stereoselective hydrolysis of racemic beta-phenylalanine ethyl ester.the ability to produce (r)- or (s)-beta-phenylalanine ethyl ester (3-amino-3-phenylpropionic acid ethyl ester, bpae) from racemic bpae through stereoselective hydrolysis was screened for in bpae-assimilating microorganisms. sphingobacterium sp. 238c5 and arthrobacter sp. 219d2 were found to be potential catalysts for (r)- and (s)-bpae production, respectively. on a 24-h reaction, with 2.5% (w/v) racemic bpae (130 mm) as the substrate and wet cells of sphingobacterium sp. 238c5 as the catalyst, 1 ...200616170530
involvement of the c-terminal tail of arthrobacter ureafaciens sialidase isoenzyme m in cleavage of the internal sialic acid of ganglioside gm1.arthrobacter ureafaciens sialidase comprises four isoenzymes, l, m1, m2 and s, of which l, m1, and m2, but not s, have the unique ability to cleave gm1 ganglioside, but the hydrolysis of gm3 and colominic acid by s occurs at a higher rate than that by l, m1 and m2. since the n-terminal amino acid sequences of l, m1, m2 and s were shown to be identical on protein sequencing, they were suggested to have arisen from the same protein through truncation at different c-terminal sites. a dna segment co ...200516169883
x-ray crystallographic analysis of 6-aminohexanoate-dimer hydrolase: molecular basis for the birth of a nylon oligomer-degrading enzyme.6-aminohexanoate-dimer hydrolase (eii), responsible for the degradation of nylon-6 industry by-products, and its analogous enzyme (eii') that has only approximately 0.5% of the specific activity toward the 6-aminohexanoate-linear dimer, are encoded on plasmid poad2 of arthrobacter sp. (formerly flavobacterium sp.) ki72. here, we report the three-dimensional structure of hyb-24 (a hybrid between the eii and eii' proteins; eii'-level activity) by x-ray crystallography at 1.8 a resolution and refin ...200516162506
reversible inhibition of copper amine oxidase activity by channel-blocking ruthenium(ii) and rhenium(i) molecular wires.molecular wires comprising a ru(ii)- or re(i)-complex head group, an aromatic tail group, and an alkane linker reversibly inhibit the activity of the copper amine oxidase from arthrobacter globiformis (agao), with k(i) values between 6 mum and 37 nm. in the crystal structure of a ru(ii)-wire:agao conjugate, the wire occupies the agao active-site substrate access channel, the trihydroxyphenylalanine quinone cofactor is ordered in the "off-cu" position with its reactive carbonyl oriented toward th ...200516157884
reinvestigation of metal ion specificity for quinone cofactor biogenesis in bacterial copper amine oxidase.the topa quinone (tpq) cofactor of copper amine oxidase is generated by copper-assisted self-processing of the precursor protein. metal ion specificity for tpq biogenesis has been reinvestigated with the recombinant phenylethylamine oxidase from arthrobacter globiformis. besides cu2+ ion, some divalent metal ions such as co2+, ni2+, and zn2+ were also bound to the metal site of the apoenzyme so tightly that they were not replaced by excess cu2+ ions added subsequently. although these noncupric m ...200516142901
characteristics of the amylase of arthrobacter psychrolactophilus.properties of the extracellular amylase produced by the psychrotrophic bacterium, arthrobacter psychrolactophilus, were determined for crude preparations and purified enzyme. the hydrolysis of soluble starch by concentrated crude preparations was found to be a nonlinear function of time at 30 and 40 degrees c. concentrates of supernatant fractions incubated without substrate exhibited poor stability at 30, 40, or 50 degrees c, with 87% inactivation after 21 h at 30 degrees c, 45% inactivation af ...200516133101
[the microbial transformation of phenanthrene and anthracene].the transformation of phenanthrene and anthracene by rhodococcus rhodnii 135, pseudomonas fluorescens 26k, and arthrobacter sp. k3 is studied. twenty-one intermediates of phenanthrene and anthracene transformation are identified by hplc, mass spectrometry, and nmr spectroscopy. p. fluorescens 26k and arthrobacter sp. k3 are found to produce a wide range of intermediates, whereas r. rhodnii 135 oxidizes phenanthrene, resulting in the formation of a sole product, 3-hydroxyphenanthrene. putative tr ...200516119849
[expression of hydantoin hydrolase gene in escherichia coli].hydantoin hydrolase with responsibility for the ring opening of hydantoin is one of the components of hydantoin utility enzymes of arthrobacter bt801 which can convert 5-benzylhydantoin into l-phenylalanine. the expression of hydantoin hydrolase gene (hyuh) is very important in elucidation of mechanisms of bio-catalysis and its application in asymmetry synthesis of amino acids. to improve the production and activity of the enzyme, the hydantoin hydrolase gene was amplified by pcr and cloned into ...200416110958
preparation of the methyl ester of hyaluronan and its enzymatic degradation.a methyl ester of hyaluronan in which the carboxyl groups were fully esterified was prepared using trimethylsilyl diazomethane. this derivative, while not depolymerized by hyaluronan lyases or hyaluronan hydrolases, was a substrate for both chondroitin aci lyase (ec 4.2.2.5) from flavobacterium heparinum and chondroitin acii lyase (ec 4.2.2.5) from arthrobacter aurescens. the major product isolated in these depolymerization reactions was methyl alpha-l-threo-hex-4-enepyranosyluronate-(1-->3)-2-a ...200516098492
crystal structure of 6-hydroxy-d-nicotine oxidase from arthrobacter nicotinovorans.the crystal structure of 6-hydroxy-d-nicotine oxidase (ec 1.5.3.6) was solved by x-ray diffraction analysis in three crystal forms at resolutions up to 1.9 a. the enzyme is monomeric in solution and also in the mother liquor but formed disulfide-dimers in all crystals. it belongs to the p-cresol methylhydroxylase-vanillyl-alcohol oxidase family and contains an fad covalently bound to the polypeptide. the covalent bond of this enzyme was the first for which a purely autocatalytic formation had be ...200516095622
[removal of harmful admixtures from a surrogate atmospheric condensate of a closed habitat with the help of a cultivated bacterial association].tested was an idea to eliminate six harmful admixtures reside in a surrogate of atmospheric condensate of closed habitats by a cultivated bacterial association. the purpose was to select members of the bacterial associations that would assimilate acetone, acetic acid, ethanol, ethyl acetate, methylamine and ammonia as free, so immobilized on insoluble cellular polyvinylformal. the resulted association of three bacterial species showed the ability to transform into end products a six-component su ...200516078425
genetic diversity of culturable bacteria in oil-contaminated rhizosphere of galega orientalis.a collection of 50 indigenous meta-toluate tolerating bacteria isolated from oil-contaminated rhizosphere of galega orientalis on selective medium was characterized and identified by classical and molecular methods. 16s rdna partial sequencing showed the presence of five major lineages of the bacteria domain. gram-positive rhodococcus, bacillus and arthrobacter and gram-negative pseudomonas were the most abundant genera. only one-fifth of the strains that tolerated m-toluate also degraded m-tolu ...200616055251
removal of high concentration of nh3 and coexistent h2s by biological activated carbon (bac) biotrickling filter.high efficiency of nh3 and h2s removal from waste gases was achieved by the biotrickling filter. granular activated carbon (gac), inoculated with arthrobacter oxydans ch8 for nh3 removal and pseudomonas putida ch11 for h2s removal, was used as packing material. under conditions in which 100% h2s was removed, extensive tests to eliminate high concentrations of nh3 emission-including removal characteristics, removal efficiency, and removal capacity of the system-were performed. the results of the ...200516051088
role of the n-terminal domain of endoinulinase from arthrobacter sp. s37 in regulation of enzyme catalysis.endoinulinase from arthrobacter sp. s37 (enia), a member of the glycoside hydrolase family 32, is unique in that, unlike other members of the family, it contains a 250-residue n-terminal domain including a "laminin-g like jelly-roll" fold. this unique n-terminal domain is here suggested to be involved in dimerization and catalysis. the essentially inactive nature of enzymes produced by n-terminal truncation (delta15, delta45, delta70, and delta250) supported the pivotal role of this unique domai ...200516046445
crystal structure of dmgo provides a prototype for a new tetrahydrofolate-binding fold.the crystal structure of dmgo (dimethylglycine oxidase) from arthrobacter globiformis in complex with folate compounds has revealed a novel thf (tetrahydrofolate)-binding fold [leys, basran and scrutton (2003) embo j. 22, 4038-4048]. this fold is widespread among folate-binding proteins. the crystal structures of aminomethyltransferase (t-protein), ygfz and trme all reveal similar thf-binding folds despite little similarity in sequence or function. the thf-binding site is highly specific for red ...200516042597
six novel arthrobacter species isolated from deteriorated mural paintings.a group of 21 bacterial strains was isolated from samples of biofilm formation in the servilia tomb (necropolis of carmona, spain) and the saint-catherine chapel (castle at herberstein, austria). a polyphasic taxonomic study of these isolates, including morphological, biochemical and chemotaxonomic characterization, rep-pcr fingerprinting, 16s rrna gene sequence analysis, dna base ratio and dna-dna relatedness studies, allocated them to the genus arthrobacter. the isolates represent six novel sp ...200516014466
[purification and characteristics of creatininase from arthrobacter sp].a creatininase produced from a arthrobacter sp. was purified 145-fold by a series of steps including heat treatment, ammonium sulfate precipitation, deae-cellulose ion-exchange and hydrophobic chromatography. the specific activity of the pure enzyme was 209u/mg. the subunit molecular mass of creatininase was estimated to be 33 700d by sds-page. the creatininase was stable in the ph range between 6.0 - 9.0 and below 60 degrees c . its km value for creatinine was estimated to be 21.14 mmol/l. the ...200516013484
microbial synthesis of chiral amines by (r)-specific transamination with arthrobacter sp. knk168.arthrobacter sp. knk168 shows (r)-enantioselective transaminase [(r)-transaminase] activity, which converts prochiral ketones into the corresponding chiral (r)-amines in the presence of an amino donor. the cultural conditions and reaction conditions for asymmetric synthesis of chiral amines with this microorganism were examined. the transaminase was inducible, and its production was enhanced by the addition of sec-butylamine and 3-amino-2,2-dimethylbutane to the culture medium. (r)-1-phenylethyl ...200616003558
a novel mammalian expression system derived from components coordinating nicotine degradation in arthrobacter nicotinovorans pao1.we describe the design and detailed characterization of 6-hydroxy-nicotine (6hnic)-adjustable transgene expression (nice) systems engineered for lentiviral transduction and in vivo modulation of angiogenic responses. arthrobacter nicotinovorans pao1 encodes a unique catabolic machinery on its plasmid pao1, which enables this gram-positive soil bacterium to use the tobacco alkaloid nicotine as the exclusive carbon source. the 6hnic-responsive repressor-operator (hdnor-o(nic)) interaction, control ...200516002786
kinetic studies of site-directed mutational isomalto-dextranase-catalyzed hydrolytic reactions on a 27 mhz quartz-crystal microbalance.a quartz-crystal microbalance (qcm) technique was applied to analyze effects of site-directed mutagenesis of a glycosidase (isomalto-dextranase) on the hydrolysis mechanism of the substrate binding (k(on), k(off), and k(d)) and the catalytic process (k(cat)), separately, by using a dextran-immobilized qcm in buffer solution. d266n, d198n, and d313n mutants, which are predicted as critical residues of the isomalto-dextranase hydrolytic activity, dramatically decreased the apparent enzyme activity ...200515996100
[construction of gene library of arthrobacter bt801 and isolation & expression of hydantoinase gene].hydantoinase can be widely used in enzymic production of various amino acids. in order to obtain the hydantoinase genes in arthrobacter bt801, its chromatosomal dna is isolated and partialy digested with sau3a i to collect fragments of about 30kb. then, this fragment is inserted into the hpa i and pst i site of cosmid pkc505. the genomic library was thus constructed by packing in vitro with lambda phage package protein and transfecting e. coli dh5alpha. a positive transformant was selected from ...200315969007
[purification and immobilization of chondroitinase from aeromonas sobria yh 311].chondroitinase has been used as an important tool in the study of the structure, function and distribution of glycosaminoglycans for many years. recently, the enzyme has been reported to be a potential enzyme for chemonucleolysis, an established treatment for intervertebral disc protrasion. in this paper, a chondroitinase had been purified from the culture supernatant of aeromonas sobria yh311 using a simple purification procedure of ammonium sulfate precipitation, qae-sephadex a50 ion exchange ...200415968993
[cloning and expression of l-n-carbamoylase gene from arthrobacter bt801 in escherichia coli].hydantoin-utility-enzyme is widely used in enzymic production of various amino acids. one of its component, carbamoylase, is responsible for the conversion of n-carbamylamino acids to corresponding amino acids, which is crucial for the stereoselectivity and rate limiting. to improve the production of the enzyme, an l-n-carbamoylase gene from arthrobacter bt801, a hydantoinase producting strain being able to convert 5-benzylhydantoin to phenylalanine, was cloned into e. coli. the gene was highly ...200315966317
use of arthrobacter davidanieli as a live vaccine against renibacterium salmoninarum and piscirickettsia salmonis in salmonids.arthrobacter davidanieli (proposed species nomenclature) is a non-pathogenic gram-variable bacterium related to, but taxonomically distinct from, renibacterium salmoninarum, the aetiological agent of bacterial kidney disease (bkd). we have demonstrated that vaccination with live a. davidanieli is effective against bkd in atlantic salmon (salmo salar) showing above 80 relative percent survival in experimental challenge trials. good protection was also demonstrated in long-term field trials where ...200515962482
production of amylase by arthrobacter psychrolactophilus.arthrobacter psychrolactophilus atcc 700733 grew with a doubling time of 1.5-2.3 h (22 degrees c) and produced up to 0.2 units/ml (soluble starch assay) of extracellular amylase in tryptic soy broth without dextrose (tsbwd) containing 0.5% or 1.0% (w/v) soluble starch or maltose as the fermentable substrate. time-course experiments in media containing soluble starch as substrate showed that amylolytic activity appeared in cultures at 24 h (after exponential growth had ceased), reached peak level ...200515931519
the crystal structure of mlc, a global regulator of sugar metabolism in escherichia coli.mlc from escherichia coli is a transcriptional repressor controlling the expression of a number of genes encoding enzymes of the phosphotransferase system (pts), including ptsg and manxyz, the specific enzyme ii for glucose and mannose pts transporters. in addition, mlc controls the transcription of malt, the gene of the global activator of the mal regulon. the inactivation of mlc as a repressor is mediated by binding to an actively transporting ptsg (eiicb(glc)). here we report the crystal stru ...200515929984
pot and field studies on bioremediation of p-nitrophenol contaminated soil using arthrobacter protophormiae rkj100.biodegradation of p-nitrophenol (pnp), a priority pollutant, was studied as a model system for bioremediation of sites contaminated with nitroaromatic/organic compounds. bioremediation of pnp-containing soil was first carried out in pots using immobilized and free cells of arthrobacter protophormiae rkj100 in order to ascertain the role of a suitable carrier material. results showed that stability of the introduced strain was enhanced upon immobilization and that the rate of pnp depletion decrea ...200515926586
a brevibacillus choshinensis system that secretes cytoplasmic proteins.brevibacillus choshinensis has previously been shown to be a useful strain for the secretion of heterologous proteins via the sec secretory pathway. this pathway involves the secretion of proteins prior to folding, whereas the alternative tat (twin-arginine translocation) pathway enables pre-folded proteins to be secreted. we have modified the signal peptide of the brevibacillus expression vector pncmo2 to accommodate a sec avoidance signal as well as the twin arginines required for secretion vi ...200415925899
an enzymatically produced novel cyclic tetrasaccharide, cyclo-{-->6)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->6)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->} (cyclic maltosyl-(1-->6)-maltose), from starch.a bacterial strain m6, isolated from soil and identified as arthrobacter globiformis, produced a novel nonreducing oligosaccharide. the nonreducing oligosaccharide was produced from starch using a culture supernatant of the strain as enzyme preparation. the oligosaccharide was purified as a crystal preparation after alkaline treatment and deionization of the reaction mixture. the structure of the oligosaccharide was determined by methylation analysis, mass spectrometry, and (1)h and (13)c nmr sp ...200515882856
substrate specificity and colorimetric assay for recombinant trzn derived from arthrobacter aurescens tc1.the trzn protein, which is involved in s-triazine herbicide catabolism by arthrobacter aurescens tc1, was cloned and expressed in escherichia coli as a his-tagged protein. the recombinant protein was purified via nickel column chromatography. the purified trzn protein was tested with 31 s-triazine and pyrimidine ring compounds; 22 of the tested compounds were substrates. trzn showed high activity with sulfur-substituted s-triazines and the highest activity with ametryn sulfoxide. hydrolysis of a ...200515870302
isolation and characterization of a diverse group of phenylacetic acid degrading microorganisms from pristine soil.a diverse range of microorganisms capable of growth on phenylacetic acid as the sole source of carbon and energy were isolated from soil. sixty six different isolates were identified and grouped according to 16s rrna gene rflp analysis. subsequent sequencing of 16s rdna from selected strains allowed further characterization of the phenylacetic acid degrading population isolated from soil. nearly half (30) of the isolates are bacillus species while the rest of the isolates are strains from a vari ...200515869782
enhanced laminin binding by alpha-dystroglycan after enzymatic deglycosylation.carbohydrate modifications are clearly important to the function of alpha-dystroglycan but their composition and structure remain poorly understood. in the present study, we describe experiments aimed at identifying the alpha-dystroglycan oligosaccharides important for its binding to laminin-1 and carbohydrate-dependent mabs (monoclonal antibodies) iih6 and via4(1). we digested highly purified skeletal muscle alpha-dystroglycan with an array of linkage-specific endo- and exoglycosidases, which w ...200515865602
depolymerisation and biodegradation of a synthetic tanning agent by activated sludges, the bacteria arthrobacter globiformis and comamonas testosteroni, and the fungus cunninghamella polymorpha.degradation of a synthetic tanning agent cnsf (a condensation product of 2-naphthalenesulfonic acid (2-nsa) and formaldehyde) by four activated sludges, two previously characterised bacterial strains, arthrobacter sp. 2ac and comamonas sp. 4bc, and the fungus cunninghamella polymorpha, was studied in batch culture at 25 degrees c by determining the changes in the concentrations of cnsf and its component monomers and oligomers (n2-n11). the loss of individual oligomers was correlated with the len ...200515865336
biodegradation of naphthalene-2-sulfonic acid present in tannery wastewater by bacterial isolates arthrobacter sp. 2ac and comamonas sp. 4bc.two bacterial strains, 2ac and 4bc, both capable of utilizing naphthalene-2-sulfonic acid (2-nsa) as a sole source of carbon, were isolated from activated sludges previously exposed to tannery wastewater. enrichments were carried out in mineral salt medium (msm) with 2-nsa as the sole carbon source. 16s rdna sequencing analysis indicated that 2ac is an arthrobacter sp. and 4bc is a comamonas sp. within 33 h, both isolates degraded 100% of 2-nsa in msm and also 2-nsa in non-sterile tannery wastew ...200515865148
characterization of pmfr, the transcriptional activator of the pao1-borne puru-mabo-fold operon of arthrobacter nicotinovorans.nicotine catabolism by arthrobacter nicotinovorans is linked to the presence of the megaplasmid pao1. genes involved in this catabolic pathway are arranged on the plasmid into gene modules according to function. during nicotine degradation gamma-n-methylaminobutyrate is formed from the pyrrolidine ring of nicotine. analysis of the pao1 open reading frames (orf) resulted in identification of the gene encoding a demethylating gamma-n-methylaminobutyrate oxidase (mabo). this gene was shown to form ...200515838033
arthrobacter ardleyensis sp. nov., isolated from antarctic lake sediment and deep-sea sediment.three psychrotrophic arthrobacter strains, isolated from antarctic lake sediment (an24, an25t) and deep-sea sediment (zx6) were studied. their 16s rrna gene sequences showed highest similarities (97.0-97.9%) with those of a. nicotianae and a. protophormiae. all three strains underwent rod-coccus morphological change, had high mol% g+c content, were aerobic to slightly anaerobic, and grew between 0 degrees c and 30 degrees c, with optimal growth temperature around 25 degrees c. the cell wall pept ...200515834596
purification and characterization of a novel sialidase from a strain of arthrobacter nicotianae.the nonpathogenic strain arthrobacter nicotianae produces two sialidase isoenzymes, na1 and na2, with molecular masses of 65 kda and 54 kda, respectively, as determined by 10% sds-polyacrylamide gel electrophoresis. na1 and na2 exhibit maximum activities at ph 4 and 5, and both show clear thermal optima at 40 degrees c. they are stable at temperatures up to 50 degrees c. the critical temperatures (t (c) = 50 degrees c and 51 degrees c) for the two isoenzymes were determined by fluorescence spect ...200515809338
a microcosm study on bioremediation of p-nitrophenol-contaminated soil using arthrobacter protophormiae rkj100.p-nitrophenol (pnp), a toxic nitroaromatic compound, can build up in soils due to extensive usage of nitrophenolic pesticides and hence needs to be removed. arthrobacter protophormiae rkj100, a pnp-degrading organism, was used in this work to study factors affecting its growth, and then evaluated for its capacity to degrade pnp in soil microcosms. molasses (10%) treated with 0.1% potassium hexacyanoferrate was found to be a suitable and cheap carbon source for inoculum preparation. induction stu ...200515806356
rhizobacteria and their potential to control fusarium verticillioides: effect of maize bacterisation and inoculum density.fusarium verticillioides is the most important seed transmitted pathogen that infects maize. it produces fumonisins, toxins that have potential toxicity for humans and animals. control of f. verticillioides colonisation and systemic contamination of maize has become a priority area in food safety research. the aims of this research were (1) to characterise the maize endorhizosphere and rhizoplane inhabitant bacteria and fusarium spp., (2) to select bacterial strains with impact on f. verticillio ...200515803383
structure of beta-glucan oligomer from laminarin and its effect on human monocytes to inhibit the proliferation of u937 cells.we analyzed the human monocyte-stimulating ability of laminarin from eisenia bicyclis, lichenan from cetraria islandica, and their oligomers depolymerized with endo-1,3-beta-glucanase from arthrobacter sp. the respective beta-glucan oligomers with different degrees of polymerization (dp) were fractionated from hydrolytic products of laminarin and lichenan using gel-filtration chromatography. the monocyte-conditioned medium pre-cultured in the presence of a fraction of beta-glucan oligomer (dp>/= ...200515784984
polyphasic taxonomic study of strain ccm 2783 resulting in the description of arthrobacter stackebrandtii sp. nov.strain ccm 2783, previously classified as representing arthrobacter aurescens, was subjected to a polyphasic taxonomic study. 16s rrna gene sequence analysis and chemotaxonomic characteristics such as peptidoglycan type a3alpha lys-ala(2), major menaquinone mk-9(h(2)) and fatty acid composition confirmed assignment of the strain to the genus arthrobacter. the results of phylogenetic analysis, dna-dna relatedness experiments and physiological and chemotaxonomic characteristics indicate that ccm 2 ...200515774666
a strain of arthrobacter that tolerates high concentrations of nitrate.a gram-positive strain identified as arthrobacter globiformis cect 4500, tolerant to up to 1 m nitrate, was isolated from the grounds of a munitions factory. under strict aerobic conditions, this bacterium used a wide variety of c-sources to obtain the energy required for growth, which took place when the nitrate concentration in the medium was below 150 mm. cells of this bacterium growing in the absence of nitrate were seen as individual cells or forming pairs, whereas cells grown in the presen ...199715765585
the structure of hormaomycin and one of its all-peptide aza-analogues in solution: syntheses and biological activities of new hormaomycin analogues.four new aza-analogues of hormaomycin 1, a secondary metabolite with interesting biological activities produced by streptomyces griseoflavus, were synthesized and subjected to preliminary tests of their antibiotic activity to provide new insights into the structure-activity relationship studies of this class of compounds. the solution structures of hormaomycin 1 and its aza-analogue 2 a were determined by nmr spectroscopy. the data exhibited a reasonably rigid conformation for both molecules, st ...200515754385
arthrobacter scleromae sp. nov. isolated from human clinical specimens.a gram-positive, coryneform bacterium was isolated from swollen scleromata of a dermatosis patient. an analysis of its phenotypic, chemotaxonomic, and genotypic characteristics showed that this bacterium is closely associated with arthrobacter oxydans and arthrobacter polychromogenes but that it belongs to a distinct species, for which the name arthrobacter scleromae sp. nov. is proposed.200515750131
microbiological degradation of pentane by immobilized cells of arthrobacter sp.the increasing production of several plastics such as expanded polystyrene, widely used as packaging and building materials, has caused the release of considerable amounts of pentane employed as an expanding agent. today many microorganisms are used to degrade hydrocarbons in order to minimize contamination caused by several industrial activities. the aim of our work was to identify a suitable microorganism to degrade pentane. we focused our attention on a strain of arthrobacter sp. which in a s ...200515727150
high-level expression of a novel amine-synthesizing enzyme, n-substituted formamide deformylase, in streptomyces with a strong protein expression system.n-substituted formamide deformylase (nfda) from arthrobacter pascens f164 is a novel deformylase involved in the metabolism of isonitriles. the enzyme catalyzes the deformylation of an n-substituted formamide, which is produced from the corresponding isonitrile, to yield the corresponding amine and formate. the nfda gene from a. pascens f164 was cloned into different types of expression vectors for escherichia coli and streptomyces strains. expression in e. coli resulted in the accumulation of a ...200515721791
molecular cloning of levan fructotransferase gene from arthrobacter ureafaciens k2032 and its expression in escherichia coli for the production of difructose dianhydride iv.to clone and overexpress a novel levan fructotransferase gene lfta from arthrobacter ureafaciens k2032.200515715649
desulphurization of dibenzothiophene and diesel oils by bacteria.to study the desulphurization of dibenzothiophene (dbt), a recalcitrant thiophenic component of fossil fuels, by two bacteria namely rhodococcus sp. and arthrobacter sulfureus isolated from oil-contaminated soil/sludge in order to use them for reducing the sulphur content of diesel oil in compliance with environmental regulations.200515715638
purification and characterization of an esterase hydrolyzing monoalkyl phthalates from micrococcus sp. ygj1.an esterase that specifically hydrolyzes medium-chain (c(3)-c(5)) monoalkyl phthalates was purified from phthalate-grown micrococcus sp. ygj1. the enzyme activity was split into two fractions by hydrophobic chromatography on phenyl sepharose, and the enzymes were purified to homogeneity from each fraction. the purified enzymes showed similar properties with respect to molecular mass (60 kda), subunit molecular mass (27 kda), n-terminal amino acid sequence, optimal ph (about 7.5), temperature-dep ...200515713880
identification of large linear plasmids in arthrobacter spp. encoding the degradation of quinaldine to anthranilate.arthrobacter nitroguajacolicus rü61a, which utilizes quinaldine as sole source of carbon and energy, was shown to contain a conjugative linear plasmid of approximately 110 kb, named pal1. it exhibits similarities with other linear plasmids from actinomycetales in that it has proteins covalently attached to its 5' ends. southern hybridization with probes for the genes encoding quinaldine 4-oxidase and n-acetylanthranilate amidase indicated that pal1 contains the gene cluster encoding the degradat ...200515699198
chemoenzymatic synthesis of cd52 glycoproteins carrying native n-glycans.a facile synthesis of homogeneous cd52 glycoproteins carrying native n-glycans was achieved using an endolycosidase-catalyzed oligosaccharide transfer as the key step. the synthesis consists of two steps: the solid phase synthesis of glcnac-cd52 and the transfer of a high-mannose type or complex type n-glycan from man(9)glcnac(2) asn or a sialglycopeptide to the glcnac-cd52, under the catalysis of the endo-beta-n-acetylglucosaminidases from arthrobacter (endo-a) and mucor hiemalis (endo-m), resp ...200515686882
isolation and characterization of a fructosyl-amine oxidase from an arthrobacter sp.an arthrobacter sp. was isolated that, when induced by fructosyl-valine, expressed a fructosyl-amine oxidase (faod) that was specific for alpha-glycated amino acids. the n-terminal amino acid sequence of the purified oxidase was determined and used to design oligonucleotides to amplify the gene by inverse pcr. expression of the gene in escherichia coli produced 0.23 units faod per mg protein, over 30-fold greater than native expression levels, with properties almost indistinguishable from the na ...200515685416
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