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effects of temperature on the macromolecular composition and find structure of psychrophilic arthrobacter species.a facultatively psychrophilic bacterium, arthrobacter si 55, grows at 20 degrees c, but growth, as measured by increase of viable cell count, is inhibited at 32 degrees c. corresponding temperatures for an obligate psychrophile, arthrobacter glacialis si 137, are 10 degrees c and 19-20 degrees c. at the higher temperatures for each organism increases of cell mass, as measured by turbidity and of dna, rna, and protein, were not inhibited. at the upper temperatures, fewer septa were formed in arth ...1977856424
purification and characterization of pyruvate carboxylase from arthrobacter globiformis. 1977851353
amino acid transport in whole cells and membrane vesicles of arthrobacter pyridinolis. 1977836027
microbiological characteristics of natural mineral water.natural, non-carbonated mineral water is, like every other natural water from a spring, never sterile. however, the microbial level is always very low. but after its bottling, the level rises rapidly and numbers of more than 10,000 to 100,000/ml can be reached. in principle 2 groups of bacteria of very different origin and properties can be found in the microbial flora of the bottled, non-carbonated mineral water. allochthonous bacteria will get into the water by contamination from the container ...1976829205
adjuvant activity of bacterial glycolipids. 1976825676
thin-layer chromatographic analysis of mycolic acid and other long-chain components in whole-organism methanolysates of coryneform and related taxa.acid methanolysates of strains representing 58 coryneform taxa were examined for mycolic acids and other long-chain constituents by thin-layer chromatography. mycolic esters were detected in the methanolysates of true corynebacteria but not in those from plant pathogenic bacteria, corynebacterium haemolyticum, corynebacterium pyogenes or from representatives of the genera arthrobacter, cellulomonas, curtobacterium, kurthia or oerskovia. thin-layer chromatography of whole-organism methanolysates ...1976825611
[mode of action of d-amino acids on the biosynthesis of peptidoglycan (author's transl)].the mechanism of growth inhibition by d-amino acids was studied. d-serine at concentrations from 0.02-0.2 m was sufficient to cause partial growth inhibition in seven species of bacteria representing the four most common types of peptidoglycan. the inhibited cells displayed morphological alterations. in the nucleotide-activated peptidoglycan precursors of these cells, d-alanine residues in position 4 and/or 5 of the peptide moiety were partially or even completely replaced by d-serine. the pepti ...1976825075
observations on lysogeny in glutamic acid bacteria.induced lysis occurred in a number of different strains of glutamic acid bacteria. mixed culture experiments indicated that induced cultures produce phages or bacteriocins. temperate phages were isolated from two related strains of brevibacterium divaricatum.1976823863
facultative wood-digesting bacteria from the hind-gut of the termite reticulitermes hesperus.among the facultative bacteria capable of growth on mesquite wood which were isolated from the asceptically dissected hind-gut of the termite reticulitermes hesperus were two strains of bacillus cereus, one strain each of arthrobacter, alcaligenes and serratia, and a very small gram-negative fermentative rod. the b. cereus strains, the serratia marcescens strain and the arthrobacter sp. grew well on a mineral salts alpha-cellusose agar. one of the bacillus cereus strains and serratia marcescens ...1976822127
identification and flagellation of coryneform bacteria from poultry litter.thirth coryneform isolates from poultry litter were identified and checked for motility and flagellation. twenty-seven formed a yellow pigment and 3 were orange. twenty-three yellow strains were found to be arthrobacter citreus, although starch was hydrolyzed by these strains. four strains, including the three mainly pale yellow isolates, grew on citrate plus an ammonium salt and were classified as a. aurescens. three orange strains were found to be brevibacterium linens. only six strains of a. ...1975811345
covalently bound fad in d-6-hydroxynicotine oxidase. immunological studies of d- and l-6-hydroxynicotine oxidase: evidence for a d-enzyme precursor.antersera prepared against both enantiozymes, d- and l-6-hydroxynicotine oxidase, formed precipitins in double diffusion tests with their respective antigens only. a mixture of the two antisera caused spur formation of the two precipitin lines obtained with the pure enzymes. antiserum to l-apoprotein reacted with native l-enzyme and l-apoprotein but not with the d-sspecific enzyme. d-6-hydroxynicotine oxidase activity was inhibited by the anti-d-antiserum, leaving the l-enzyme fully active, whil ...1975809438
evidence for the occurrence of specific iron (iii)-binding compounds in near-shore marine ecosystems.aqueous extracts of samples from representtive types of near-shore marine ecosystems were examined for growth-promoting activity for the siderochrome auxotroph, arthrobacter jg-9. blue-green algal mat and sea grass samples gave strongly positive responses. relative to desferal (ciba pharmaceutical co., summit, n.j.), concentrations up to 110 ng/g (wet weight) of sample were found. in contrast, extracts of anaerobic muds or macroalgae gave no detectable activity. the hydroxamate type of iron chel ...1975809005
microflora and invert sugars in juice from healthy tissue of stored sugarbeets.bacterial populations increased in juice of healthy tissue of sugarbeet roots stored at 5 c. average counts showed a sixfold increase after 150 days of storage. invert sugar levels increased over threefold in "american 4 hybrid a" and remained fairly constant in "mono-hy d-2." the former cultivar also had significantly higher bacterial colony counts than the latter before 90 days of storage. of 36 isolates identified, 16 were pseudomonas spp. including p. chlororaphis; 6 bacillus spp. including ...1975808167
the fine structure of the branched alpha-d-glucan from the blue-green alga anacystis nidulans: comparison with other bacterial glycogens and phytoglycogen.the fine structure of the glycogen from the blue-green alga anacystis nidulans has been examined. after selective hydrolysis of all (1 yields 6)-alpha-d linkages by a bacterial isoamylase, the resulting mixture of linear chains was subjected to gel permeation chromatography. for purposes of comparison, the glycogens from escherichia coli and arthrobacter sp., amylopectin, phytoglycogen from sweet corn, and shell-fish glycogen were treated similarly. the profiles of the unit chains of a nidulans ...1975806344
a numerical taxonomic study of coryneform and related bacteria.two hundred and thirty-three strains of coryneform bacteria, including representatives of the genera arthrobacter, brevibacterium, cellulomonas, corynebacterium, erysipelothrix, jensenia, kurthia, listeria, microbacterium, mycobacterium, nocardia and propionibacterium and other related bacteria, were studied using 173 morphological, physiological and biochemical tests. the bacteria were grown on a soil extract medium which allowed growth of all the strains, and all were incubated at 30 degrees c ...1975805825
chondroitinase-producing bacteria in natural habitats.a search was undertaken for bacteria which degrade chondroitin sulfate in nature and to find bacteria with a usefully high rate of chondroitinase (chsase) productivity. first, 253 chsase-producing bacteria were obtained from aquatic and land environments in japan by aerobic and anaerobic screening methods. identification according to bergey's manual of determinative bacteriology or bain and shewan (1968) permitted assignment of the majority of the isolates to seven genera, aeromonas, vibrio, fla ...1975803822
restriction-enzyme-cleavage maps of bacteriophage m13. existence of an intergenic region on the m13 genome.replicative form dna of bacteriophage m13 was cleaved into specific fragments by an endonuclease isolated from hemophilus aegyptius (endor.haeii) and an endonuclease from arthrobacter luteus (endor.alui). the fragments were ordered as to construct a circular map of the phage m13 genome by: (a) using each fragment as a primer for the synthesis in vitro of its respective neighbour and (b) digesting the isolated fragments with the hemophilus aegyptius enzyme endor.haeii or the hemophilus aphirophil ...1976786638
[isolation of bacteria assimilating ethanol from natural and industrial substrates]. 1975765694
enzymic assay for lecithin in amniotic fluid.we describe an enzymic method for determining lecithin in amniotic fluid. phospholipase d from savoy cabbage is used to liberate choline from lecithin, and the liberated choline is oxidized to betaine and hydrogen peroxide by choline oxidase from arthrobacter globiformis. the hydrogen peroxide forms a colored complex with phenol and 4-aminoantipyrene in the presence of horseradish peroxidase, and this color is measured spectrophotometrically. the method is relatively easy, precise, and accurate, ...1979761344
morphogenetic expression of arthrobacter globiformis 425 in continuous culture with carbon or biotin limitation.when deprived of biotin, arthrobacter globiformis 425 exhibits abnormal morphology (large, branched forms of variable size) and a retardation of its normal growth rate. in chemostat cultures, when cells were grown under glucose limitation, the morphology was normal (coccoids or rods) at specific growth rates between 0.05 and 0.125 h-1 (doubling times between 14 and 5.5 h, respectively) at 25 degrees c. the coccoid-to-rod morphogenesis occurs at a specific growth rate of 0.11 h-1. at the same spe ...1978754873
effect of freezing and thawing on survival of three bacterial isolates from an arctic soil.three isolates, a pseudomonas sp., bacillus sp., and arthrobacter sp., which had been isolated from a meadow soil at devon island, canada, were subjected to freezing and thawing at low rates under various conditions. when cells were frozen in sand or soil, survival was dependent on moisture level, storage time, and thaw rate. pseudomonas m216 was most susceptible to freeze-thaw damage under these conditions. arthrobacter m51 was the most resistant of the three isolates when frozen in sand or soi ...1978747810
effect of starvation on survival of three bacterial isolates from an arctic soil.three isolates, a pseudomonas sp., a bacillus sp. and an arthrobacter sp., which had been isolated from a meadow soil at devon island, canada, were subjected to starvation under varying conditions. the viabilities of the three isolates during starvation for 30 days in a carbon-free medium was assessed after the organisms had been grown continuously at varying rates in carbon- and nitrogen-limited media at 5 and 15 degrees c. pseudomonas m216 was the most resistant to starvation stress, bacillus ...1978747809
effect of temperature, growth rate, and nutrient limitation on the yield and composition of three bacterial isolates from an arctic soil grown in continuous culture. 1978747808
fad is covalently attached to peptidyl-trna during cell-free synthesis of 6-hydroxy-d-nicotine oxidase.the process, by which fad is attached covalently to the 6-hydroxy-d-nicotine oxidase apoprotein in d-nicotine-induced cells of arthrobacter oxidans was studied in vitro. [3h]adenine-labelled fad prepared biosynthetically in clostridium kluyveri was incorporated into the 6-hydroxy-d-nicotine oxidase molecule during cell-free translation. fad rather than fmn or riboflavin was thus shown to be the flavin derivative transferred to the polypeptide chain. after short-term protein synthesis on ribosome ...1978738274
induction and regulation of neuraminidase synthesis in arthrobacter sialophilus.a variety of n-acetylneuraminic acid (acneu) derivatives and analogs were examined as inducers of the extracellular neuraminidase of arthrobacter sialophilus. neuraminidase inductions were primarily studied with tryptone-yeast extract-grown cells after washing and resuspension in a defined replacement medium. the addition of readily metabolizable carbon sources to the latter, such as 0.1% casein hydrolysate, glutamate, or glucose, enhanced enzyme synthesis. enzyme appearance occurred after a lag ...1978721778
regulation of flavoprotein synthesis studied in vivo in a riboflavin-requiring mutant of arthrobacter oxidans.the biosynthesis of two flavoproteins, 6-hydroxy-d-nicotine oxidase with covalently bound fad and 6-hydroxy-l-nicotine oxidase containing non-covalently bound fad, was studied in wild-type cells and in a riboflavin-requiring mutant of arthrobacter oxidans. in the mutant cells, the rate of synthesis and the maximal activity level of both enzymes after induction by nicotine depended on the amount of added riboflavin. the low rate of synthesis in the presence of 2 micron riboflavin could be enhance ...1978718370
kinetics of accumulation of a photodynamically induced cell-surface polypeptide in a species of arthrobacter.cells of a species of arthrobacter were incubated in the light with methylene blue, a dye that sensitizes photooxidative reactions by the production of singlet oxygen. an early and major response by the cells to these conditions was stimulation of synthesis of a single cell-surface polypeptide, 21,000 daltons in mass. the rate of synthesis of this polypeptide reached a maximal level about 30 min after the start of illumination. as a consequence, the amount of this polypeptide increased at least ...1978711669
[effect of exogenous acetyl group acceptors on cholinesterase biosynthesis in arthrobacter simplex cells].the presence of active acetyl or butyryl groups and their acceptors in the growth medium was found to be necessary for the high rate of cholinesterase biosynthesis in the cells of arthrobacter simplex var. cholinesterasus. the active acetyl and butyryl groups are formed upon hydrolysis of acetylcholine and butyrylcholine as well as in the course of glucose metabolism. the following acids were shown to be the acceptors of the acetyl and butyryl groups: butyric, succinic, fumaric, malic acids and, ...1978703648
mechanism of arthrobacter sialophilus neuraminidase: the binding of substrates and transition-state analogs. 1978697876
growth characteristics of three bacterial isolates from an arctic soil.three bacterial isolates, a pseudomonas sp., a bacillus sp., and an arthrobacter sp., commonly isolated from a hummocky sedge-moss meadow at devon island, n.w.t., canada, were selected for further taxonomic characterization and for a study of the effects of temperature and limiting carbon source on growth. pseudomonas m216 resembled p. putida and bacillus m153, b. carotarum. arthrobacter m51 had growth-factor requirements which were more complex than those of any named species of that genus. the ...1978688099
isolation and characterization of morphogenetic mutants of arthrobacter crystallopoietes.mutants of arthrobacter crystallopoietes that exhibited altered ability to undergo the normal sphere-to-rod-to-sphere morphogenetic cycle were isolated. the procedure used to isolate these mutants involved velocity sedimentation in a sterile sucrose gradient to separate morphogenesis-deficient spherical cells from rod-shaped cells capable of normal morphogenesis. three classes of mutants were obtained: (i) those that cannot form rods, (ii) those that cannot form long rods, and (iii) those that f ...1978681281
bacterial xanthine oxidase from arthrobacter s-2.arthrobacter s-2, originally isolated by enrichment on xanthine, produced high levels of xanthine oxidase activity, requiring as little as a 20-fold purification to approach homogeneity with some preparations. molecular oxygen, ferricyanide, and 2,6-dichlorophenol-indophenol served as electron acceptors, but nicotinamide adenine dinucleotide did not. the enzyme was relatively specific when compared with previously studied xanthine-oxidizing enzymes, but at least one purine was observed to be oxi ...1978681279
[microflora of the low-mineralized waters of the truskavets deposit].waters of the truskavetz deposit with a low content of mineral substances were found to contain considerable amounts of microorganisms; upto millions of cells per 1 ml. sulphate-reducing, dentrifying and iron bacteria were detected in the waters. the following genera were represented and isolated: pseudomonas, bacillus, nocardia, arthrobacter, brevibacterium, corynebacterium, micrococcus, candida, cryptococcus, rhodotorula, hansenula, torulopsis, trichosporon. many of the studied strains assimil ...1978672688
[aqueous petroleum-oxidizing arthrobacter].water petroleum oxidizing bacteria of arthrobacter ceroformans as well as their lipid composition are described. on a medium containing hexadecane, all the strains produce wax in large amounts (upto 70% of total free lipids) consisting by 95% of cetyl palmitate. fatty acids are represented mainly by palmitic acid but comprise also unsaturated c16:1 and c18:1 acids. mycolic acids have not been detected. the significance of lipid composition for the identification of arthrobacteria is discussed.1978672687
susceptibility of ganglioside gm1 to a new bacterial neuraminidase. 1978658424
catabolism of 2,4,5-trimethyoxybenzoic acid and 3-methoxycrotonic acid.4-methoxygentisic acid was an intermediate formed when arthrobacter degraded, 2,4,5-trimethoxybenzoic acid. isolates of pseudomonas and arthrobacter from soil grew at the expense of 3-methoxycrotonic acid. evidence is presented that enzymatic hydration, with elimination of methanol, accounted for replacement of the methoxyl group of 3-methoxycrotonic acid and also of one methoxyl group of 2,4,5-trimethoxybenzoic acid.1978646361
enzymatic determination of phospholipase d activity with choline oxidase.a new enzymatic method was developed for the assay of phospholipase d [phosphatidylcholine phosphatidohydrolase ec 3.1.4.4] from cabbage leaves using choline oxidase from arthrobacter globiformis cells. the method was based on the estimation of choline by the following series of enzymatic reactions after ending the phospholipase d reaction: choline + 202 + h2o choline oxidase betaine + 2h2o2 2h2o2 + phenol + 4-aminoantipyrine peroxidase quinoneimine dye + 4h2o the amount of choline was proportio ...1978641031
purification and properties of arthrobacter neuraminidase.neuraminidase (ec 3.2.1.18) from an arthrobacter species was purified homogeneity by conventional procedures (yield approx. 1 mg/1) and was judged to be homogeneous by sodium dodecyl sulfate gel electrophoresis. gel electrofocusing of neuraminidase revealed 1 major band (85-90%), pi 5.35 +/- 0.05, and 6 minor bands, whose pi ranged from 5.25 to 5.70, and each of which had catalytic activity. arthrobacter neuraminidase is a monomeric glycoprotein of molecular weight 88 000, has an apparent km of ...1978629985
purification and characterization of choline oxidase from arthrobacter globiformis.choline oxidase was purified from the cells of arthrobacter globiformis by fractionations with acetone and ammonium sulfate, and column chromatographies on deae-cellulose and on sephadex g-200. the purified enzyme preparation appeared homogeneous on disc gel electrophoresis. the enzyme was a flavoprotein having a molecular weight of approx. 83,000 (gel filtration) or approx. 71,000 (sodium dodecyl sulfate--polyacrylamide disc gel electrophoresis) and an isoelectric point (pi) around ph 4.5. iden ...1977599154
[identification of some of fermentation of phenanthrene by microorganisms belonging to the genus arthrobacter]. 1977597413
studies of the glycine cleavage enzyme system in brain from infants with glycine encephalopathy.glycine content and enzyme activity of the glycine cleavage system were compared in autopsied brain from five infants dying with glycine encephalopathy and four control infants, including two with other types of hyperglycinemia. glycine content was elevated 2- to 8-fold and glycine cleavage enzyme activity was undetectable in the brains of the glycine encephalopathy patients. glycine content and enzyme activity were normal in the brains of the control patients, including one with ketotic hypergl ...1977593763
distribution of neuraminidase in arthrobacter and its purification by affinity chromatography.neuraminidase [sialidase, ec 3.2.1.18] was found to be widely distributed in bacteria belonging to arthrobacter. among these bacteria, arthrobacter ureafaciens, a. oxydans, and a. aurescens produced relatively potent neuraminidase activities. for the production of this enzyme, not only colominic acid, a homopolymer of n-acetylneuraminic acid, but also n-acetylneuraminic acid, the reaction product of this enzyme, are effective as sources of carbon. an affinity adsorbent specific for neuraminidase ...1977591509
degradation of (+/-)-synephrine by arthrobacter synephrinum. oxidation of 3,4-dihydroxyphenylacetate to 2-hydroxy-5-carboxymethyl-muconate semialdehyde.1. cell-free extracts of arthrobacter synephrinum catalyse the oxidation of 3,4-dihydroxy-phenylacetate. 2. the product of oxidation was characterized as 2-hydroxy-5-carboxymethylmuconate semialdehyde from its chemical behaviour as well as from nuclear-magnetic-resonance spectra. 3. a 3,4-dihydroxyphenylacetate 2,3-dioxygenase (ec 1.13.11.15) was partially purified from a. synephrinum. 4. the enzyme had a km of 25 micrometer towards its substrate and exhibited typical michaelis-menten kinetics. ...1977588248
adenylate nucleotide levels and energy charge in arthrobacter crystallopoietes during growth and starvation.the adenylate nucleotide concentrations, based on internal water space, were determined in cells of arthrobacter crystallopoietes during growth and starvation and the energy charge of the cells was calculated. the energy charge of spherical cells rose during the first 10 h of growth, then remained nearly constant for as long as 20 h into the stationary phase. the energy charge of rod-shaped cells rose during the first 4 h of growth, then remained constant during subsequent growth and decreased i ...1979518238
adenosine triphosphate pool levels and endogenous metabolism in arthrobacter crystallopoietes during growth and starvation.the adenosine triphosphate (atp) content of arthrobacter crystallopoietes was measured during growth, starvation and recovery from starvation. during exponential growth of the cells as spheres in a glucose slats medium, the level of atp per cell remained constant at 8.0 x 10(-10) micrograms/cell. morphogenesis to rodshaped cells and an increased growth rate following addition of casein hydrolysate was accompanied by an almost two-fold increase in the atp level. as division of the rod-shaped cell ...1979518237
mass-transfer effects on the rate of isomerization of d-glucose into d-fructose, catalyzed by whole-cell immobilized glucose isomerase.the investigated catalyst system consists of immobilized arthrobacter cells containing the enzyme glucose isomerase, which catalyzes the isomerization of glucose into fructose. the internal structure of the catalyst was determined from electrom microscope photographs of replicas of freeze-etched catalyst. on the basis of the photographs a model for the internal structure of the catalyst was proposed. this structure was subsequently used to describe the reaction including mass-transfer effects. i ...1979486717
effect of oxygen on batch and continuous cultures of a nitrogen-fixing arthrobacter sp.growth, acetylene reduction, and respiration rate were studied in batch and continuous cultures of arthrobacter fluorescents at different oxygen partial pressures. the optimum po2 values for growth and acetylene reduction were 0.05 and 0.025 atm, respectively, but microorganisms can tolerate higher po2 values. the growth of cultures provided with combined nitrogen was dependent on oxygen availability, and strict anaerobic conditions did not support growth. acetylene reduction of a population gro ...1979476551
structural homologies in alanine-rich acidic ribosomal proteins from procaryotes and eucaryotes.the amino acid composition and amino-terminal sequence have been determined for the alanine-rich, acidic ribosomal 'a' protein (equivalent to escherichia coli l7/l12) from three procaryotic cell types that live under extreme environmental conditions (arthrobacter glacialis, clostridium pasteurianum, and bacillus stearothermophilus) as well as from wheat germ, a eucaryote source. these data are compared with previously published 'a' protein sequences from other procaryotes and eucaryotes. all the ...1979476516
[microbiologic studies of a spodumene deposit].a wide spectrum of heterotrophic and autotrophic microorganisms was detected in the zones of decomposition of spodumene and bed rocks, pegmatites and shales, in the spodumene deposit. the following aerobic microorganisms which did not from spores predominated in the deposit: arthrobacter globiformis, a. pascens, a. simplex, nocardia globerula, pseudomonas fluorescens, ps. putida, ps. testosteronii. the following specific bacterial groups were found: thionic, sulfate reducing, and nitrifying bact ...1979470634
action of arthrobacter ureafaciens sialidase on sialoglycolipid substrates. mode of action and highly specific recognition of the oligosaccharide moiety of ganglioside gm1.a new bacterial sialidase (n-acetylneuraminate glycohydrolase, ec 3.2.1.18) isolated from the culture filtrate of arthrobacter ureafaciens was characterized in detail with respect to its action on sialoglycolipids. strong electrolytes had a reversible inhibitory effect on the action of the enzyme on brain gangliosides in accordance with debye-hückel effect of ionic environment on ionic activity, and resulted in an acidic shift and a broadening of the ph optimum. both ionic and non-ionic detergen ...1979468792
induction of the phosphoenolpyruvate: hexose phosphotransferase system associated with relative anaerobiosis in an obligate aerobe.arthrobacter pyridinolis possesses alternative transport systems for d-fructose: a respiration-coupled transport system whereby d-fructose transport occurs with concomitant oxidation of l-malate, and a phosphoenolpyruvate: d-fructose phosphotransferase system. studies of d-fructose uptake by whole cells in the presence and absence of cyanide demonstrate that respiration-coupled transport is used almost exclusively during the first half of logarithmic growth, after which it accounts for only 15-2 ...1979456352
plasmid-determined 2-hydroxypyridine utilization by arthrobacter crystallopoietes.arthrobacter crystallopoietes has the ability to utilize 2-hydroxypyridine (2-hp) as a source of carbon and nitrogen and forms a blue extracellular pigment when grown in the presence of 2-hp. ultracentrifugal analyses of pigment producing (pig+) and pigment nonproducing (pig-) strains of a. crystallopoietes revealed the presence of plasmid material in both strains. recovery of plasmid dna from pig+ strains is two or three times greater than from pig- strains. the molecular weight of plasmid dna ...1979455149
emulsifier of arthrobacter rag-1: chemical and physical properties.the extracellular emulsifier of arthrobacter rag-1 was deproteinized by hot phenol treatment and purified by fractional precipitation with (nh(4))(2)so(4). the active fraction, precipitating between 30 and 35% saturation [ef-rag(uet) wa], appeared to be homogeneous by immunodiffusion and sedimentation analysis. ef-rag(uet) wa had an intrinsic viscosity of 750 cm(3)/g, a sedimentation constant of 6.06s, a diffusion constant of 5.25 x 10(-8) cm(2) s(-1), and a partial molar volume of 0.712 cm(3) g ...1979453822
emulsifier of arthrobacter rag-1: specificity of hydrocarbon substrate.the purified extracellular emulsifying factor produced by arthrobacter rag-1 (ef-rag) emulsified light petroleum oil, diesel oil, and a variety of crude oils and gas oils. although kerosine and gasoline were emulsified poorly by ef-rag, they were converted into good substrates for emulsification by addition of aromatic compounds, such as 2-methylnaphthalene. neither aromatic nor aliphatic fractions of crude oil were emulsified by ef-rag; however, mixtures containing both fractions were emulsifie ...1979453821
emulsifier of arthrobacter rag-1: determination of emulsifier-bound fatty acids. 1979446732
dna-dna homology studies among strains of arthrobacter and brevibacterium.sixteen named strains of arthrobacter and two strains of brevibacterium were investigated by nucleic acid hybridisation. the arthrobacter strains show homology values ranging between 11 and 55% to the type strain a. globiformis dsm 20124 (atcc 8010), indicating only a low to moderate relationship. two strains of a. globiformis, dsm 20124 and dsm 20125, exhibit only poor relationship to one another (30%). among all the arthrobacter strains the homology data range between 10 to 70% demonstrating s ...1979443991
[hydrocarbon-oxidizing microflora of uncontaminated seawater].the distribution of hydrocarbon-oxidizing microflora in noncontaminated sea waters was studied in the northern region of the pacific ocean in the vicinity of the copper island. the total number of microorganisms was assayed as well as the number of heterotrophic, oligocarbophilic and hydrocarbon-oxidizing microorganisms. oligocarbophilic bacteria were the most abundant group (from 120 to 24,000 cells per 1 ml), and predominated in 12 stations among 23. the number of heterotrophic organisms was n ...1979440168
covalently bound flavin as prosthetic group of choline oxidase. 1979435313
evidence for selective sulfhydryl reactivity in cytochalasin a--mediated bacterial inhibitions. 1979435298
physiological responses of bacteria to cytochalasin a: effects on growth, transport, and enzyme induction.cytochalasin a at 5 to 25 microgram/ml (1.0 x 10(-5) to 5.2 x 10(-5) m) inhibited the growth of three gram-positive bacteria, arthrobacter sialophilus, staphyloccus aureus, and bacillus amyloliquifaciens, but had little or no effect on the growth of three gram-negative bacteria, excherichia coli, pseudomonas maltophilia, and aeromonas proteolytica. a. sialophilus and s. aureus recovered spontaneously from cytochalasin a-mediated growth inhibition after a considerable lag period, which was depend ...1979422516
[distribution of the taxonomic traits in coryneform bacteria]. 1978418310
structural and immunochemical studies on d-arabino-d-mannans and d-mannans of mycobacterium tuberculosis and other mycobacterium species.serologically active d-arabino-d-mannas ([alpha]d, +82 degrees approximately 89 degrees; ratio of d-arabinose to d-mannose, 1-2:1) were isolated from the soluble fraction of disintegrated cells of m. tuberculosis, m. smegmatis, and several other mycobacterium species. these arabinomannans had similar structures, consisting of alpha-(1 leads to 5)-linked d-arabinose residues and alpha-(1 leads to 6)-, and (1 leads to 2)-linked d-mannose residues. methylation and enzymic degradation studies using ...1977413830
isolation, synthesis, and biological activity of five metabolites of danazol.metabolites of danazol (17 alpha-pregna-2,4-dien-20-yno[2,3-d]isoxazol-17-ol), an orally effective pituitary gonadotropin inhibitory agent devoid of estrogenic and progestational activites, were isolated from urine of a female subject who had taken danzol orally at a dose of 800 mg/day for 7 days, the metabolites isolated were 17-hydroxy-17alpha-pregn-4-en-20-yn-3-one (11), 17-hydroxy-2alpha-(hydroxymethyl)-17alpha-pregn-4-3n-20-yn-3-one (5), 17-hydroxy-2-(hydroxymethyl)-17alpha-pregna-1,4-dien- ...1977403283
comparative analysis of three guinea pig satellite dna's by restriction nucleases.the structures of guinea pig satellite dnas i, ii, and iii have been analyzed by digestion with seven restriction nucleases. from the cleavage patterns it is obvious that the long-range periodicities in these three satellites differ rather characteristically satellite i is fairly resistant to six nucleases and gives only a number of weak discrete bands which do not show a simple regularity. by the restriction nuclease from arthrobacter luteus, however, it is cleaved extensively and yields very h ...1977403072
chitin structures of the cell walls of synchronously grown virgin cells of saccharomyces cerevisiae.the ability of a lytic beta-glucanase of arthrobacter gjm-1 to dissolve cell walls of saccharomyces cerevisiae with exception of the chitin-containing fraction was employed for the isolation of chitin-rich residues of the cell walls of synchronously growing populations of virgin cells. electron microscopical examination of such wall residues isolated from cells at various stages of the budding cycle showed that the first phase of chitin deposition in the wall corresponds to the formation of an a ...1979396723
degradation of even-numbered reduced and non-reduced hyaluronate oligosaccharides with d-glucuronic acid or n-acetyl-d-glucosamine as non-reducing terminal by chondroitin abc and ac lyases.chondroitin abc and ac lyases split hexosaminidic linkages in galactosaminoglycans and hyaluronic acid. even-numbered oligosaccharides from hyaluronic acid with either d-glucuronic acid or n-acetylglucosamine in non-reducing position were used, prior to and after reduction with sodium borohydride, as substrates for chondroitin abc and ac lyases. these substrates allowed elucidation of the effects of the nearest neighborhood of the bond to be split on the action of the enzymes. the results indica ...1979387569
rate of drying and the survival of microorganisms.the survival rate of cells of the genera arthrobacter, pseudomonas, mycobacterium, escherichia, micrococcus and saccharomyces when counted immediately after fast or slow drying (20 minutes and 24 hours, respectively) was rather similar. however, after prolonged periods of dry storage, the number of viable cells after slow drying was much higher as compared with the rapidly dried cells. investigations with escherichia coli demonstrated this phenomenon only when more than about 8 mg of water per 1 ...1979386942
exocellular, microbial polysaccharides. 1979380276
production of microbial polysaccharides. 1978356538
[cadaverine is an intermediate in the biosynthesis of arthrobactin and ferrioxamine e (author's transl)].cadaverin was more readily incorporated than lysine into arthrobactin from arthrobacter pascens and into ferrioxamin e from streptomyces glaucescens. from a racemic mixture only the l-isomer of lysine is incorporated. the l-lysine decarboxylase activity was measured in vivo and in vitro. the enzyme from arthrobacter pascens is not inducable by lysine and completely repressed by 5.10(-6) m fe3+. in klebsiella pneumoniae, the producer of aerobactin, only a very low activity of l-lysine decarboxyla ...1978354550
cell-free synthesis of 6-hydroxy-d-nicotine oxidase containing covalently bound fad. 1978342273
a microperfusion chamber for studying the growth of bacterial cells. 1977338570
enzymes of the yeast lytic system produced by arthrobacter gjm-1 bacterium and their role in the lysis of yeast cell walls.yeast lytic system produced by arthrobacter gjm-1 bacterium during growth on baker's yeast cell walls contains a complete set of enzymes which can hydrolyze all structural components of cell walls of saccharomyces cerevisiae. chromatographic fractionation of the lytic system showed the presence of two types of endo-beta-1,3-glucanase. rapid lysis of isolated cell walls of yeast was induced only by endo-beta-1,3-glucanase exhibiting high affinity to insoluble beta-1,3-glucans and releasing lamina ...1977337691
ribosomal protein s1/s1a in bacteria. 1977330231
new microbial growth factor.a screening procedure was used to isolate from soil a penicillium sp., two bacterial isolates, and a streptomyces sp. that produced a new microbial growth factor. this factor was an absolute growth requirement for three soil bacteria. the penicillium sp. and one of the bacteria requiring the factor, an arthrobacter sp., were selected for more extensive study concerning the production and characteristics of the growth factor. it did not seem to be related to the siderochromes. it was not present ...1977327929
release of microorganisms from soil with respect to transmission electron microscopy viewing and plate counts. 1977326180
l-serine dehydratase from arthrobacter globiformis.1. l-serine dehydratase (ec 4.2.1.13) was purified 970-fold from glycine-grown arthrobacter globiformis to a final specific activity of 660micronmol of pyruvate formed/min per mg of protein. 2. the enzyme is specific for l-serine; d-serine, l-threonine and l-cysteine are not attacked. 3. the time-course of pyruvate formation by the purified enzyme, in common with enzyme in crude extracts and throughout the purification, is non-linear. the reaction rate increases progressively for several minutes ...1977322657
characterization of distinct segments in mouse satellite dna by restriction nucleases.the long-range periodicity of mouse satellite dna has been analyzed by digestion with five restriction nucleases. with all nucleases tested, a major repeat unit approximately 245 nucleotide pairs became apparent. minor registers of shorter length were also detected. the total number of cleavage sites per haploid genome for each restriction enzyme as well as their positions relative to each other were determined. while endo r-ecorii was known to cleave all of the satellite dna, the other four res ...1977321221
mapping and length measurements of restriction enzyme fragments by electron microscopy.1. we have mapped by electron microscopy the dna-fragments formed by the action of the restriction endonuclease from arthrobacter luteus of phi x 174 replicative form dna. these fragments were separated by polyacrylamide gel electrophoresis and hybridized to phix 174 single stranded dna. the partial duplex molecules were inspected in the electron microscope. in this way the relative order of eleven fragments ranging in size from approximately 100 to 1000 nucleotide pairs has been established and ...1977321023
tetrahydrofolate-dependent biosynthesis of ribothymidine in transfer ribonucleic acids of gram-positive bacteria.trimethoprim, an inhibitor that prevents tetrahydrofolate-dependent transmethylation reactions inbacteria, was used in a comparative study to discriminate between two possible biosynthetic pathways, either the s-adenosylmethionine or the tetrahydrofolate-dependent formation of ribothymidine (rt) in transfer ribonucleic acids (trna's) of several strains of gram-positive and gram-negative microorganisms. rt-deficient trna's accumulate in trimethoprim-treated gram-positive streptococcus faecium, st ...1977318638
nature of transformation of hydrocortisone by cells incorporated into polyacrylamide gel.the ability of mycobacterium globiforme 193 cells immobilized in polyacrylamide gel (paag) to transform soluble and microcrystalline hydrocortisone was investigated under conditions to periodic aeration. it was found that the specific 3-oxosteroid-delta'-dehydrogenase activity of immobilized cells hardly differs from that of free cells and averages 0.06 mumole/mg cells . min; the quantitative and qualitative composition of the transformation products formed by free and immobilized cells was iden ...1979297469
[evaluation of the cholinesterase activity of bacteria on a solid nutrient medium]. 1975241005
[distribution of pyrimidine blocks in the dna of brevibacterium linens, arthrobacter globiformis, nocardia corallina and nocardia rubra].the nucleotide composition and the frequency of pyrimidine blocks were studied in dna of the following bacteria: brevibacterium linens (weignamm, 1910) breed, 1953; arthrobacter globiformis (conn, 1928) conn et dimmick, 1947; nocardia corallina (bergey et al., 1923) waksman et henrici, 1948; nocardia rubra (krassilnikov, 1949) waksman et henrici, 1948. these organisms are classed by some microbiologists as mycobacteria (the mycobacteriaceae family) while other authors regard them as representati ...1975241002
transformation of morphine by resting cells and cell-free systems of arthrobacter sp.morphine can be transformed into 14-hydroxymorphine and a related unidentified material by resting cells of an arthrobacter species. cell-free extracts containing the transforming enzyme(s) have been obtained. o2, fe2+, and reduced nicotinamide adenine dinucleotide stimulate the transformation.1975240309
bacteriostasis in soils sterilized by gamma irradiation and in reinoculated sterilized soils.an agar-disc method was used to compare the bacteriostatic properties of five soils with those of samples of the same soils sterilized by gamma irradiation. for three of the soils, bacteriostasis was removed either partially or entirely by sterilization. bacteriostasis was completely restored to sterile soil by reinoculating it with natural soil. all the reinoculation treatments restored the level of bacteriostasis equally effectively, whatever the origin of the soil used as inoculum.1975235358
crystallization and some properties of chondroitinase from arthrobacter aurescens.a strain of arthrobacter aurescens which secretes a large amount of chondroitinase into a culture broth, was isolated from soil. the chondroitinase was purified 380-fold over culture broth in 24% yield and crystallized. some properties of the purified enzyme were studied and described: thermal stability (below 45 degrees), ph stability (ph 4.9 to 7.4), optimum temperature (50 degrees), and optimum ph (ph 6.0). chrondroitin sulfate a and c, chondroitin, and hyaluronic acid were split by the enzym ...1975234466
catabolic pathways of coryneforms, nocardias, and mycobacteria. 1979227323
physical mapping of bk virus dna with saci, mboii, and alui restriction endonucleases.a new restriction endonuclease, saci from streptomyces achromogenes cleaves bk virus (strain mm) dna into 3 fragments, whereas mboii from moraxella bovis and alui from arthrobacter luteus give 22 and 30 fragments, respectively. all these specific dna fragments were ordered and mapped on the viral genome by two methods first, by the reciprocal digestion method using uniformly 32p-labeled dna; and second, by the partial digestion technique using the single-end 32p-labeled dna. this study, together ...1978215783
periodic cyclic amp uptake by synchronously grown cells of nocardia restricta and arthrobacter globiformis. 1978212312
regulation of cyclic amp levels in arthrobacter crystallopoietes and a morphogenetic mutant.the extracellular levels of cyclic amp (camp), camp phosphodiesterase activity, and adenylate cyclase activity were measured at various intervals during growth and morphogenesis of arthrobacter crystallopoietes. there was a significant rise in the extracellular camp level at the onset of stationary phase, and this rise coincided with a decrease in intracellular camp. the phosphodiesterase activity measured in vitro increased in the early exponential phase of growth as intracellular camp decrease ...1978207674
2-hydroxypyridine metabolism and pigment formation in three arthrobacter species.three species of the genus arthrobacter, a. crystallopoietes, a. pyridinolis, and a. viridescens, have the capabilities to utilize 2-hydroxypyridine (2-hp) as the sole source of carbon and nitrogen for growth and to produce an extracellular crystalline pigment from this substrate. degradation of 2-hp by cell-free extracts requires the presence of both reduced nicotinamide adenine dinucleotide and molecular oxygen and is stimulated by flavin mononucleotide, suggesting the presence of a monooxygen ...1977199576
oxidative pathway of choline to betaine in the soluble fraction prepared from arthrobacter globiformis.one strain of bacteria which showed high h2o2-generating activity was isolated from soil and characterized as arthrobacter globiformis based on its morphological, nutritional, and physiological characteristics. the activities of h2o2 generation, nad reduction and oxygen consumption in the bacterial cells were examined using choline, betaine aldehyde or betaine as substrate. choline was oxidized to betaine aldehyde under aerobic conditions in a reaction coupled with h2o2 generation and oxygen con ...1977197073
[features of secondary metabolism in microorganisms]. 1977194631
control of morphogenesis in arthrobacter crystallopoiets: effect of cyclic adenosine 3',5'-monophosphate.the intracellular levels of cyclic adenosine 3',5'-monophosphate (cyclic amp) were measured at various intervals during growth and morphogenesis in arthrobacter crystallopoietes. cyclic amp levels remained relatively constant throughout growth in spherical cells grown in glucose-based media. immediately after inoculation of spheres from glucose- to succinate-containing media, a 30-fold increase in intracellular cyclic amp was detected. this dramatic rise in cyclic amp preceded the observed chang ...1977190210
metabolism of l-rhamnose in arthrobacter pyridinolis.in arthrobacter pyridinolis, a respiration-coupled transport system for l-rhamnose caused accumulation of free l-rhamnose, while a phosphoenolpyruvate: l-rhamnose phosphotransferase system caused accumulation of l-rhamnose i-phosphate (levinson & krulwich, 1974). the pathways for subsequent metabolism of l-rhamnose and l-rhamose i-phosphate have now been investigated. arthrobacter pyridinolis contains an inducible l-rhamnose isomerase and l-rhamnulokinase, as well as a constitutive l-rhamnulose ...1976182906
arthrobacter luteus restriction endonuclease recognition sequence and its cleavage map of sv40 dna.the nucleotide sequence at the cleavage site of the restriction endonuclease isolated from arthrobacter luteus (alu) has been determined. the endonuclease cleaves at the center of a palindromic tetranucleotide sequence to give even-ended duplex dna fragments phosphorylated at the 5'-end. the endonuclease cleaves sv40 form i dna into 32 fragments. the order and sizes of these fragments have been determined to provide an alu cleavage map of the sv40 genome.1976182213
a comparative analysis of the ultrastructure of hydrocarbon-oxidizing micro-organisms.the ultrastructure of a variety of micro-organisms was compared after growth on hydrocarbon and non-hydrocarbon substrates. hydrocarbon-grown organisms were characterized by the presence of intracellular electron-transparent inclusions which in many cases appeared membrane-bound. with one exception, non-hydrocarbon-grown organisms did not contain electron-transparent inclusions. insignificant amounts of poly-beta-hydroxybutyric acid were produced by the hydrocarbon-grown micro-organisms. after g ...1976181527
stability of enzymes in starving arthrobacter crystallopoietes.cell-free extracts prepared from spherical and rod-shaped cells of arthrobacter crystallopoietes were assayed for enzymes during various periods of starvation. the level of nadh oxidase dropped to 20 and 30%, respectively, in spherical and rod-shaped cells during the first 1 to 2 days of starvation and then remained constant for 9 days. catalase activity decreased continuously and reached a low level in 9 days. enzymes involved in glucose metabolism and the tricarboxylic acid cycle were stable f ...1976180237
specific cleavage and physical mapping of simian-virus-40 dna by the restriction endonuclease of arthrobacter luteus.simian virus 40 (sv40) dna (strain 776) is cleaved by the restriction endonuclease from arthrobacter luteus into 32 specific fragments including 20 large pieces designated alu-a through t as well as 12 minor products named alu m1 through m8. these were mapped on the sv40 genome by double digestion experiments. alu fragments were treated with hind enzymes and vice versa. similar reciprocal digestions were also carried out with hae iii enzyme. in this way a detailed cleavage map of the sv40 genome ...1976173549
survival of human enteric and other sewage microorganisms under simulated deep-sea conditions.the survival of pure cultures of escherichia coli, streptococcus faecalis, clostridium perfringens, and vibrio parahaemolyticus under simulated deep-sea conditions of low temperature (4 c), seawater, and hydrostatic pressures ranging from 1 to 1,000 atm was determined over a period exceeding 300 h. the viability of e. coli and total aerobic bacteria in seawater-diluted raw sewage subjected to these deep-sea conditions was also measured. there was a greater survival of both e. coli and s. faecali ...1975169733
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