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studies on the effect of inoculation of activated sludge with bacteria actively degrading hydrocarbons on the biodegradation of petroleum products.eighteen strains of bacteria were isolated from activated sludge purifying petroleum-refining wastewaters. these strains were plated on solidified mineral medium supplemented with oil fraction in concentration 1000 mg/l. four of the strains that grew best in the presence of oil were selected for further studies. the strains were identified based on bonde's scheme and microscopic observations. three of them belonged to the genus arthrobacter and one to the genus micrococcus. stationary cultures o ...200212588103
transgenics of an elite indica rice variety pusa basmati 1 harbouring the coda gene are highly tolerant to salt stress.transgenic lines of indica rice were generated by agrobacterium-mediated transformation with the choline oxidase ( coda) gene from arthrobacter globiformis. choline oxidase catalyses conversion of choline to glycine betaine. glycine betaine is known to provide tolerance against a variety of stresses. molecular analyses of seven independent transgenic lines as performed by southern, northern and western hybridization revealed integration and expression of the transgene as well as inheritance in t ...200212582870
[microbial destruction of harmful organic contaminants inherent to atmosphere of living space].the investigation into the microbial destruction of harmful organic contaminants in the air of habitable rooms was performed with the assumption that microorganisms have labile metabolism and synthesize inducible enzymes involved in transformation of organics compounds. based on our observations, methylobacterium d-08 and arthrobacter pastens are able to synthesize inducible enzymes and transform ethanol, acetic acid, acetone, and aldehyde. association of these microorganisms does not reveal acu ...200212572127
arthrobacter strain vai-a utilizes acyl-homoserine lactone inactivation products and stimulates quorum signal biodegradation by variovorax paradoxus.many proteobacteria produce acyl-homoserine lactones (acyl-hsls) and employ them as dedicated cell-to-cell signals in a process known as quorum sensing. previously, variovorax paradoxus vai-c was shown to utilize diverse acyl-hsls as sole sources of energy and nitrogen. we describe here the properties of a second isolate, arthrobacter strain vai-a, obtained from the same enrichment culture that yielded v. paradoxus vai-c. although strain vai-a grew rapidly and exponentially on a number of substr ...200312571011
evaluation of broiler litter with reference to the microbial composition as assessed by using 16s rrna and functional gene markers.very little is known about the microbial composition of animal bedding wastes, including poultry litter, and what is known has been deduced from standard culture methods, by which some fastidious organisms that exist in the environment may not be detected. we evaluated the bacterial composition of poultry litter by using a combination of culture and molecular detection. total aerobic bacteria in poultry litter were detected by culture at 10(9) cfu/g of material. enteric bacteria such as enteroco ...200312571010
chemoenzymatic synthesis of high-mannose type hiv-1 gp120 glycopeptides.a chemoenzymatic approach to the synthesis of glycoforms of hiv-1 gp120 glycopeptides is described. thus, the high-mannose type glycopeptides [gp120 (336-342)] containing man(9), man(6) and man(5) moieties, respectively, were synthesized in satisfactory yields via transglycosylation to the acetylglucosaminyl peptide, using the recombinant arthrobacter endo-beta-n-acetyl-glucosaminidase (endo-a) as the key enzyme.200312565922
[the solubilization of four insoluble phosphates by some microorganisms].four insoluble phosphates of ferric phosphate (fe-p), aluminum phosphate (al-p), fluorapatite (fap) and rock phosphate (rp) were used as a sole phosphorus resource for some phosphate-solubilizing microorganisms. it was found that there was significant difference in solubilizing these phosphates by the tested isolates. the fungi normally were more powerful than the bacteria in dissolving the phsophates. the microorganisms generally solubilized more phosphate when supplied with no3- than with nh4+ ...200212557403
[studies on hydantoinase producing conditions of arthrobacter k1108].the hydantoinase-producing conditions from strain arthrobacter k1108 were investigated. it is shown that the hydantoinase in the strain is intracellular and inducible. its optimal inducer is 5-benzylhydantoin, while 5-indolylmethylhydantoin and 5-phenylhydantoin cannot induce the production of hydantoinase in k1108. a gratuitous inducer was designed, with which the hydantoinase production is 343% as much as that with 5-benzylhydantoin. the media for culturing the bacteria were screened and optim ...200212557399
[microbial succession on lignite along with weathering].six different weathered lignite samples were examined by scanning electron microscopy. few microorganisms were observed on lignit just excavated and only spores and short hyphae were observed on lignite samples excavated 5 months, 1 year and 4 years before sampling. when lignite samples were moistened with distilled water and incubated for 10 days, actinomycetes proliferated significantly on lignite samples that were just excavated or excavated 5 months before sampling. the growth of bacteria wa ...199812548918
microbial diversity and resistance to copper in metal-contaminated lake sediment.contamination of habitats with heavy metals has become a worldwide problem. we describe herein the analysis of lake sediment contaminated with high concentrations of copper as a consequence of mine milling disposal over a 100-year period. copper concentrations in the sediment were found to vary with depth and ranged from 200 to 5500 ppm. analysis of the microbial community with t-rflp identified a minimum of 20 operational taxonomic units (otu). t-rflp analysis along a depth profile detected as ...200312545313
role of copper ion in bacterial copper amine oxidase: spectroscopic and crystallographic studies of metal-substituted enzymes.the role of the active site cu(2+) of phenylethylamine oxidase from arthrobacter globiformis (agao) has been studied by substitution with other divalent cations, where we were able to remove >99.5% of cu(2+) from the active site. the enzymes reconstituted with co(2+) and ni(2+) (co- and ni-agao) exhibited 2.2 and 0.9% activities, respectively, of the original cu(2+)-enzyme (cu-agao), but their k(m) values for amine substrate and dioxygen were comparable. x-ray crystal structures of the co- and n ...200312537504
mrna differential display in a microbial enrichment culture: simultaneous identification of three cyclohexanone monooxygenases from three species.mrna differential display has been used to identify cyclohexanone oxidation genes in a mixed microbial community derived from a wastewater bioreactor. thirteen dna fragments randomly amplified from the total rna of an enrichment subculture exposed to cyclohexanone corresponded to genes predicted to be involved in the degradation of cyclohexanone. nine of these dna fragments are part of genes encoding three distinct baeyer-villiger cyclohexanone monooxygenases from three different bacterial speci ...200312514013
citricoccus muralis gen. nov., sp. nov., a novel actinobacterium isolated from a medieval wall painting.a gram-positive, aerobic, spherical actinobacterium, designated strain 4-0(t), was isolated from a medieval wall painting and characterized to determine its taxonomic position. the peptidoglycan of strain 4-0(t) was of type a4alpha, with lysine as the diagnostic cell wall diamino acid and an interpeptide bridge of lys-gly-glu. its quinone system contained predominantly mk-9(h2) (64%) and its polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, four ...200212508874
novel reversible indole-3-carboxylate decarboxylase catalyzing nonoxidative decarboxylation.after enrichment culture with indole-3-carboxylate in static culture, a novel reversible decarboxylase, indole-3-carboxylate decarboxylase, was found in arthrobacter nicotianae fi1612 and several molds. the enzyme reaction was examined in resting-cell reactions with a. nicotianae fi1612. the enzyme activity was induced specifically by indole-3-carboxylate, but not by indole. the indole-3-carboxylate decarboxylase of a. nicotianae fi1612 catalyzed the nonoxidative decarboxylation of indole-3-carb ...200212506977
biodegradation of the mixture of 2,4,6-trichlorophenol, 4-chlorophenol, and phenol by a defined mixed culture.two new strains, pseudomonas sp. tcp114 degrading 2,4,6-trichlorophenol (tcp) and arthrobacter sp. cpr706 degrading 4-chlorophenol (4-cp), were isolated through a selective enrichment procedure. both strains could also degrade phenol. the degradability of one component by a pure culture was strongly affected by the presence of other compounds in the medium. for example, when all three components (tcp, 4-cp, and phenol) were present in the medium, a pure culture of cpr706 could not degrade any of ...199712501340
[mercury-resistant bacteria from permafrost sediments and prospects for their use in comparative studies of mercury resistance determinants].mercury-resistant bacteria were isolated from permafrost sediments of kolyma lowland and canada existing over five thousand to two million years. their content was shown to vary within the range 0.001-2.9% and to depend on the amount of mercury in sampling sites (coefficient of correlation 0.75). a collection of mercury-resistant bacterial strains was created. in this collection, various representatives of both gram-positive bacteria (bacillus, exiguobacterium, micrococcus, arthrobacter) and gra ...200212500685
exposure to airborne microorganisms and endotoxin in a potato processing plant.microbiological air sampling was performed in a big potato processing plant located in eastern poland. air samples for determination of concentrations of microorganisms, dust and endotoxin were collected at 6 sites in the division producing potato flakes and meal from dried potato pulp and at 2 sites in the division producing potato syrup from imported starch. the concentrations of total airborne microorganisms were within a range of 28.3-93.1 x 10(3) cfu/m(3). mesophilic bacteria were dominant ...200212498592
simultaneous horizontal gene transfer of a gene coding for ribosomal protein l27 and operational genes in arthrobacter sp.phylogenetic analysis of bacterial l27 ribosomal proteins showed that, against taxonomy, the l27 protein from the actinobacteria arthrobacter sp. clusters with protein sequences from the bacillus group. the l27 gene clusters in the arthrobacter sp. genome with six genes responsible for creatinine and sarcosine degradation. phylogenetic analyses of orthologue proteins encoded by three of these genes also showed a phylogenetic relationship with bacillus species. comparisons between the synonymous ...200212486522
characterization of the 4-carboxy-4-hydroxy-2-oxoadipate aldolase gene and operon structure of the protocatechuate 4,5-cleavage pathway genes in sphingomonas paucimobilis syk-6.the protocatechuate (pca) 4,5-cleavage pathway is the essential metabolic route for degradation of low-molecular-weight products derived from lignin by sphingomonas paucimobilis syk-6. in the 10.5-kb ecori fragment carrying the genes for pca 4,5-dioxygenase (ligab), 2-pyrone-4,6-dicarboxylate hydrolase (ligi), 4-oxalomesaconate hydratase (ligj), and a part of 4-carboxy-2-hydroxymuconate-6-semialdehyde dehydrogenase (ligc), we found the ligk gene, which encodes 4-carboxy-4-hydroxy-2-oxoadipate (c ...200312486039
metabolism of gamma-hexachlorocyclohexane by arthrobacter citreus strain bi-100: identification of metabolites.growth characteristics of the aerobic bacterial strain arthrobacter citreus bi-100 in mineral salts medium with gamma-hexachlorocyclohexane (gamma-hch) as the sole source of carbon and degradation of gamma-hch by the strain are reported. the highest yield of the bacteria is observed at a gamma-hch concentration of 100 mg/l. at this concentration, the bacteria entered the exponential phase of growth without any lag. at 8 h of growth, no residual hch, but its metabolites, was detectable in the med ...200012483592
cystite formation of arthrobacter ureafaciens nric 0157(t).the cystite formation of arthrobacter ureafaciens nric 0157(t) was studied by the use of a newly designed ct medium composed of 2.0% d-glucose, 0.28% nh(4)h(2)po(4), 0.136% k(2)hpo(4) 0.136% kh(2)po(4), 0.0005% mgso(4).7h(2)o, and 0.0007% cacl(2).2h(2)o. cystites are drumstick-shaped and oval cells and are larger than vegetative cells. cystites are gram-negative, whereas vegetative cells are gram-positive by the koh reaction. the concentration and ratio of k(+) and mg(2+) in ct medium mainly aff ...200012483589
the formation and structures of cystites of arthrobacter ureafaciens nric 0157t induced by antibiotics.the cystite formation of arthrobacter ureafaciens nric 0157(t) was induced by some antibiotics, and the addition of tetracycline at a lag phase was effective for cystite formation in ypm liquid medium. cystites differed from vegetative cells in the cell wall structure, protein content, and water content. furthermore, the characteristics and structures of cystites induced by tetracycline were similar to those of cystites produced by nutritional imbalance in ct medium. consequently, various trigge ...200112483560
isolation of arthrobacter strains on the basis of their cystite formation. 200212469299
high affinity iron-uptake systems in vibrio damsela: role in the acquisition of iron from transferrin.in this work, the high affinity iron-acquisition systems displayed by virulent and avirulent strains of vibrio damsela have been investigated. this species is an autochthonous member of marine ecosystems that can behave as an opportunistic pathogen for fish and mammals. all strains tested (i) were able to grow under the restricted conditions imposed by the iron chelators transferrin (tf) and eddha, (ii) secreted siderophores of hydroxamic type, other than aerobactin and desferal, that were able ...199712452589
impact of protozoan grazing on bacterial community structure in soil microcosms.the influence of grazing by a mixed assemblage of soil protozoa (seven flagellates and one amoeba) on bacterial community structure was studied in soil microcosms amended with a particulate resource (sterile wheat roots) or a soluble resource (a solution of various organic compounds). sterilized soil was reinoculated with mixed soil bacteria (obtained by filtering and dilution) or with bacteria and protozoa. denaturing gradient gel electrophoresis (dgge) of pcr amplifications of 16s rrna gene fr ...200212450833
arthrobacter aurescens tc1 metabolizes diverse s-triazine ring compounds.arthrobacter aurescens strain tc1 was isolated without enrichment by plating atrazine-contaminated soil directly onto atrazine-clearing plates. a. aurescens tc1 grew in liquid medium with atrazine as the sole source of nitrogen, carbon, and energy, consuming up to 3,000 mg of atrazine per liter. a. aurescens tc1 is metabolically diverse and grew on a wider range of s-triazine compounds than any bacterium previously characterized. the 23 s-triazine substrates serving as the sole nitrogen source i ...200212450818
aari, a restriction endonuclease from arthrobacter aurescens ss2-322, which recognizes the novel non-palindromic sequence 5'-cacctgc(n)4/8-3'.a new type ii restriction endonuclease aari has been isolated from arthrobacter aurescens ss2-322. aari recognizes the non-palindromic heptanucleotide sequence 5'-cacctgc(n)4/8-3' and makes a staggered cut at the fourth and eighth bases downstream of the target duplex producing a four base 5'-protruding end. aari activity is stimulated by oligodeoxyribonucleotide duplexes containing an enzyme-specific recognition sequence.200212409482
intact golgi synthesize complex branched o-linked chains on glycoside primers: evidence for the functional continuity of seven glycosyltransferases and three sugar nucleotide transporters.we examined the functional co-localization and continuity of glycosyltransferases and sugar nucleotide transporters in the golgi of two chinese hamster ovary (cho) cell lines that synthesize different types of o-linked oligosaccharides. cho cells normally synthesize primarily sia2,3galbeta1,3galnac- on glycoproteins. cho cells transfected with core-2 glcnac transferase (core 2) can synthesize glycoproteins containing branched o-linked oligosaccharides with poly-n-acetyllactosamines. cho lines in ...200112376727
bioremediation of an industrial effluent containing monocrotophos.almost 30% of the precious agricultural output of india is lost owing to pest infestation. in india, pesticide consumption for protecting crops is about 3% of the total world consumption. monocrotophos (mcp), an organophosphorus insecticide, is widely used to control insects on crops. being readily water soluble and highly toxic, its removal from wastewater generated during manufacture becomes inevitable. bioremediation of wastewater containing mcp by arthrobacter atrocyaneus, bacillus megateriu ...200212232665
cultivation-dependent characterization of bacterial diversity from british columbia forest soils subjected to disturbance.bacteria from forest surface organic matter and mineral soil horizons were cultivated using four methods and characterized by fatty acid methyl ester (fame) analysis. soil samples from a british columbia ministry of forests long-term soil productivity (ltsp) installation were collected during winter and summer from two disturbance treatments (whole-tree harvesting with no soil compaction (plot n) and whole-tree harvesting plus complete surface organic matter removal with heavy soil compaction (p ...200212224563
capillary electrophoresis of cr(vi) reducer arthrobacter oxydans.rapid and effective separation of bacteria arthrobacter oxydans was performed using capillary electrophoresis. for optimal separation of bacteria the influence of buffer concentration, ph and applied voltage were studied. it was found that the most appropriate conditions for electrophoretic mobility measurements are as follows: applied voltage 6-14 kv; buffer concentration 5-10 mm ph 6-8. at the stationary phase of growth there are always two main heterogeneous peaks. they are connected with the ...200212210506
purification and characterization of aminobutyraldehyde dehydrogenase from arthrobacter sp. tmp-1.aminobutyraldehyde dehydrogenase was purified to essentially homogeneity from putrescine-grown cells of arthrobacter sp. tmp-1. the molecular weights of the enzyme and its subunit were 201,000 and 51,000, respectively, suggesting that the enzyme is a tetramer of identical subunits. the apparent michaelis constants (k(m)) for 4-aminobutyraldehyde, 3-aminopropionaldehyde and 4-guanidinobutyraldehyde were approximately 65, 150, and 85 microm, respectively. linear fatty aldehydes also tested were le ...200212186751
tolerance of bacteria to organic solvents.organic-solvent-tolerant bacteria are a relatively novel group of extremophilic microorganisms. they overcome the toxic and destructive effects of organic solvents due to the presence of various adaptive mechanisms. extensive studies done on the toluene tolerance of certain pseudomonas strains have led to an understanding of the mechanisms of organic solvent tolerance involving novel adaptations such as the toluene efflux pumps, cis-trans isomerisation of membrane fatty acids, rapid membrane rep ...200212160316
towards the development of selective amine oxidase inhibitors. mechanism-based inhibition of six copper containing amine oxidases.four substrate analogs, 4-(2-naphthyloxy)-2-butyn-1-amine (1), 1,4-diamino-2-chloro-2-butene (2), 1,6-diamino-2,4-hexadiyne (3), and 2-chloro-5-phthalimidopentylamine (4) have been tested as inhibitors against mammalian, plant, bacterial, and fungal copper-containing amine oxidases: bovine plasma amine oxidase (bpao), equine plasma amine oxidase (epao), pea seedling amine oxidase (psao), arthrobacter globiformis amine oxidase (agao), escherichia coli amine oxidase (ecao), and pichia pastoris lys ...200212153561
biomineralization of an organophosphorus pesticide, monocrotophos, by soil bacteria.to study biomineralization of monocrotophos (mcp) and identify the metabolites formed during biodegradation.200212147070
optimization of protease activity of alkaliphilic bacteria isolated from an alkaline lake in india.alkaliphilic bacteria belonging to the genera bacillus, staphylococcus, micrococcus, pseudomonas and arthrobacter isolated from sediment samples of the alkaline lonar lake situated in the buldhana district of maharashtra state, india, were studied for the production of protease activity. among the 54 isolates obtained, arthrobacter ramosus and bacillus alcalophilus exhibited high protease activity using soyacake as a sole source of carbon and nitrogen. protease activity was optimum at 1% initial ...200212146649
x-ray snapshots of quinone cofactor biogenesis in bacterial copper amine oxidase.the quinone cofactor tpq in copper amine oxidase is generated by posttranslational modification of an active site tyrosine residue. using x-ray crystallography, we have probed the copper-dependent autooxidation process of tpq in the enzyme from arthrobacter globiformis. apo enzyme crystals were anaerobically soaked with copper; the structure determined from this crystal provides a view of the initial state: the unmodified tyrosine coordinated to the bound copper. exposure of the copper-bound cry ...200212134140
the structure of l-hydantoinase from arthobacter aurescens leads to an understanding of dihydropyrimidinase substrate and enantio specificity.l-hydantoinase from arthrobacter aurescens (l-hyd) is a member of the dihydropyrimidinases which in turn belong to the cyclic amidases. dihydropyrimidinases catalyze the reversible hydrolytic ring opening of dihydropyrimidines as the second step in the catabolism of pyrimidines. in biotechnology, their hydroloytic activity on five-membered cyclic diamides (hydantoins) is used in the enantio-specific production of amino acids from racemic hydantoins. l-hyd differs from most of the other dihydropy ...200212093275
electron spin resonance study of copper biosorption by bacteria.the biosorption of copper by bacteria was studied by using esr spectroscopy. among bacteria tested, arthrobacter nicotianae has the most excellent ability to sorb copper. the biosorption of copper by arthrobacter cells was so rapid, affected by the solution ph, and obeys the langmuir adsorption equilibrium. the electron spin resonance (esr) spectra of cu(ii) in bacterial cells are axial type, having a major absorption to higher field at gperpendicular and lesser absorption to lower field at gpar ...200212092584
response of furniture factory workers to work-related airborne allergens.the aim of this work was to determine the reactivity of furniture factory workers to microbial allergens associated with wood dust. allergological examinations by skin and precipitin tests were performed in 48 workers employed in a factory producing furniture from fibreboards and chipboards, and in 32 healthy urban dwellers not exposed to organic dusts (referents). the skin test was performed by the intradermal method with the saline extracts of the cultures of 3 microbial species (rahnella sp., ...200212088404
exposure to airborne microorganisms in furniture factories.microbiological air sampling was performed in 2 furniture factories located in eastern poland. in one factory furniture were made from fibreboards and chipboards while in the other from beech wood. it was found that the concentration of total microorganisms (bacteria + fungi) in the air of the facility using beech wood for furniture production (mean 10.7 x (3) cfu/m(3), range 3.3 27.5 x (3) cfu/m(3)) was significantly higher (p < 0.01) compared to microbial concentration in the facility using fi ...200212088403
arthrobacter roseus sp. nov., a psychrophilic bacterium isolated from an antarctic cyanobacterial mat sample.strain cms 90rt, a red-pigmented bacterium, was isolated from a cyanobacterial mat sample from a pond located in mcmurdo, antarctica. based on its chemotaxonomic and phylogenetic properties, strain cms 90r(t) was identified as a member of group i of arthrobacter. it shared 16s rdna similarity of 98% with arthrobacter oxydans atcc 14358t and arthrobacter polychromogenes atcc 15216t, while dna-dna similarities determined for these three organisms were less than 70%. it also differed from all 17 re ...200212054218
[the role of low-molecular-weight nitrogen compounds in the osmotolerance of rhodococcus erythropolis and arthrobacter globiformis].investigations showed that rhodococcus erythropolis e-15 and arthrobacter globiformis 2f cells respond to osmotic shock by increasing the synthesis of free amino acids, primarily glutamic acid (80% of the intracellular free amino acid pool). the osmoprotective role of glutamic acid follows from its beneficial effect on the growth of bacteria in high-salinity media. it was found that the addition of this amino acid to the growth medium at a concentration of 2 mm shortened the lag phase and increa ...200212024813
utilization of xylitol dehydrogenase in a combined microbial/enzymatic process for production of xylitol from d-glucose.the production of xylitol from d-glucose occurs through a three-step process in which d-arabitol and d-xylulose are formed as the first and second intermediate product, respectively, and both are obtained via microbial bioconversion reactions. catalytic hydrogenation of d-xylulose yields xylitol; however, it is contaminated with d-arabitol. the aim of this study was to increase the stereoselectivity of the d-xylulose reduction step by using enzymatic catalysis. recombinant xylitol dehydrogenase ...200212018283
flow-through chemiluminescence sensor using immobilized histamine oxidase from arthrobacter crystallopoietes kait-b-007 and peroxidase for selective determination of histamine.a flow sensor with immobilized oxidases is proposed for the determination of histamine in fish meat. chemiluminometric measurement of histamine was based on the luminol reaction with hydrogen peroxide produced by immobilized histamine oxidase (ec 1.4.3.-.) and peroxidase (ec 1.11.1.7.) within a flow cell. histamine oxidase was found in cells of arthrobacter crystallopoietes kait-b-007 isolated from soil. the oxidase and peroxidase were coimmobilized covalently on tresylated hydrophilic vinyl pol ...200111990588
response of endophytic bacterial communities in potato plants to infection with erwinia carotovora subsp. atroseptica.the term endophyte refers to interior colonization of plants by microorganisms that do not have pathogenic effects on their hosts, and various endophytes have been found to play important roles in plant vitality. in this study, cultivation-independent terminal restriction fragment length polymorphism analysis of 16s ribosomal dna directly amplified from plant tissue dna was used in combination with molecular characterization of isolates to examine the influence of plant stress, achieved by infec ...200211976096
[bacterial succession on n-alkanes under the conditions of sulfate reduction].the dynamics of species composition of a hydrocarbon-oxidizing bacteriocenosis of a ground suspension of mozhaisk reservoir has been studied. the bacteriocenosis was undergoing development in a paraffin film (model association composed of sulfate-reducing bacteria and hydrocarbon-oxidizing bacteria). the type of bacterial succession did not depend on the depth, from which ground samples were collected. two microbial species (pseudomonas sp. and arthrobacter globiformis) were absolutely dominant. ...200211962208
arthrobacter nasiphocae sp. nov., from the common seal (phoca vitulina).an unknown gram-positive, catalase-positive, strictly aerobic, rod-shaped bacterium was isolated from the nasal cavities of two common seals. chemical analysis revealed the presence in the bacterium of a hitherto unknown cell-wall murein [type: l-lys-l-ala2-gly(2-3)-l-ala (gly)]. comparative 16s rrna gene sequencing showed that the unidentified rod was related to the arthrobacter group of organisms, although sequence divergence values of >3% from established members of this genus indicated that ...200211931170
l-methionine degradation potentialities of cheese-ripening microorganisms.volatile sulphur compounds are major flavouring compounds in many traditional fermented foods including cheeses. these compounds are products of the catabolism of l-methionine by cheese-ripening microorganisms. the diversity of l-methionine degradation by such microorganisms, however, remains to be characterized. the objective of this work was to compare the capacities to produce volatile sulphur compounds by five yeasts, geotrichum candidum, yarrowia lipolytica, kluyveromyces lactis, debaryomyc ...200111928962
mechanistic aspects of the covalent flavoprotein dimethylglycine oxidase of arthrobacter globiformis studied by stopped-flow spectrophotometry.dimethylglycine oxidase (dmgo) is a covalent flavoenzyme from arthrobacter globiformis that catalyzes the oxidative demethylation of dimethylglycine to yield sarcosine, formaldehyde, and hydrogen peroxide. stopped-flow and steady-state kinetic studies have been used to study the reductive and oxidative half-reactions using dimethylglycine and o2 as substrates. the reductive half-reaction is triphasic. the rate of the fast phase is dependent on substrate concentration, involves flavin reduction, ...200211926836
[studies on bioconversion conditions and stereoselectivity of arthrobacter k1108 hydantoinase].the bioconversion conditions of hydantoinase of arthrobacter k1108 were studied. it was shown that the optimum temperature of the enzyme is 55 degrees c, and the optimum ph is 7.0. the enzyme can be activated by co2+ and fe2+, while inhibited by ca2+. the optimal substrate of the hydantoinase is 5-benzylhydantoin, while 5-phenylhydantoin and 5-indolylmethylhydantoin cannot effectively digested, showing a high specificity on the substrates. an investigation on the hydantoinase stereoselectivity m ...200111910755
inhibition of copper amine oxidases by pyridine-derived aldoximes and ketoximes.the reactions of pea diamine oxidase (psao) and 2-phenylethylamine oxidase from arthrobacter globiformis (agao) with pyridine-derived oximes were studied. pyridine carbaldoximes and alkyl pyridyl ketoximes act as strong non-competitive inhibitors of the enzymes. the inhibition constants k(i) of these compounds vary between 10(-4) and 10(-5) m, for agao and some of the studied oximes were found even micromolar k(i) values. the presence of pyridine moiety in the studied compounds has remarkable in ...200111879727
conversion of liposomal 4-androsten-3,17-dione by a. simplex immobilized cells in calcium pectate.arthrobacter simplex atcc 6946 free and immobilized cells were assayed for their ability to convert 4-androsten-3,17-dione (ad) to 1,4-androstadien-3,17-dione (add) in aqueous and liposomal media. bioconversions were carried out in a 100 ml flask containing 25 ml of ad liposomal or aqueous medium for 3h, and ad concentrations ranging from 0.3 to 1.0 mm were tested. ad/add ratios in samples were determined by hplc. biotransformation of substrate entrapped in multilamellar vesicles (mlv) was demon ...200211867272
isolation, characterization and diuron transformation capacities of a bacterial strain arthrobacter sp. n2.a bacterial strain able to transform diuron was isolated from a soil by enrichment procedures. strain isolation was realized by plating on minimal-agarose medium spread with this herbicide and selecting the colonies surrounded by a clear thin halo. one strain was characterized and identified as an arthrobacter sp. it metabolized diuron and the final transformation product, 3,4-dichloroaniline, was produced in stoichiometric amounts. the transformation of diuron at different concentrations was mo ...200211838431
biotransformation of phenylurea herbicides by a soil bacterial strain, arthrobacter sp. n2: structure, ecotoxicity and fate of diuron metabolite with soil fungi.in order to assess the influence of the aromatic substitution on the ability of a soil bacterial strain, arthrobacter sp. n2, to degrade phenylurea herbicides, biotransformation assays were performed in mineral medium with resting cells of this soil bacterial strain on three phenylurea herbicides (diuron, chlorotoluron and isoproturon). each herbicide considered, led to the formation of only one metabolite detected by hplc analysis. after isolation, the metabolites were identified by nmr and ms, ...200211838430
growth of moderately halophilic bacteria isolated from sea water using phenol as the sole carbon source.moderately halophilic bacteria utilizing phenol as the sole carbon source were isolated by selective enrichment from sea water. the isolate (gram-negative motile rods) was identified as deleya venusta. it grew well in the presence of up to 1600 mg/l of phenol and 8% nacl under aerobic conditions. when the cells were treated with chloramphenicol prior to the addition of phenol they did not utilize added phenol, even after prolonged incubation. thus, the enzymes necessary for phenol metabolism app ...200111830940
repeated inoculation as a strategy for the remediation of low concentrations of phenanthrene in soil.phenanthrene, a polycyclic aromatic hydrocarbon, becomes increasingly unavailable to microorganisms for degradation as it ages in soil. consequently, many bioaugmentation efforts to remediate polycyclic aromatic hydrocarbons in soil have failed. we studied the effect of repeatedly inoculating a soil with a phenanthrene-degrading arthrobacter sp. on the mineralization kinetics of low concentrations of phenanthrene. after the first inoculation, the initial mineralization rate of 50 ng/g phenanthre ...200111826901
[effects of heavy charged particles on strains of microorganisms-producers of biologically active substances].effects of heavy charged particles on strains of bacillus thuringiensis ssp. kurstaki z-52 and arthrobacter oc-1 have been studied. evidence was obtained that heavy charged particles impact the morphologocultural and physiological properties of culture. as noted, the conditions of orbital flight may be considered a source of mutagenic effects on cultures of microorganisms.200011816408
enzymes of dimethylsulfone metabolism and the phylogenetic characterization of the facultative methylotrophs arthrobacter sulfonivorans sp. nov., arthrobacter methylotrophus sp. nov., and hyphomicrobium sulfonivorans sp. nov.novel methylotrophic arthrobacter and hyphomicrobium species are described. constitutive membrane-associated dimethylsulfone- and dimethylsulfoxide-reductases were found in arthrobacter methylotrophus strain tga and hyphomicrobium sulfonivorans strain s1. enzyme activities increased during growth with dimethylsulfone or dimethylsulfoxide, respectively, and different ratios of activity with different growth substrates indicated that they are separate enzymes. sds-page showed some membrane-associa ...200211807567
the trans-anethole degradation pathway in an arthrobacter sp.a bacterial strain (ta13) capable of utilizing t-anethole as the sole carbon source was isolated from soil. the strain was identified as arthrobacter aurescens based on its 16 s rrna gene sequence. key steps of the degradation pathway of t-anethole were identified by the use of t-anethole-blocked mutants and specific inducible enzymatic activities. in addition to t-anethole, strain ta13 is capable of utilizing anisic acid, anisaldehyde, and anisic alcohol as the sole carbon source. t-anethole-bl ...200211805095
[strains of pseudomonas fluorescens 3 and arthrobacter sp. 2--degradation of polycyclic aromatic hydrocarbons].pseudomonas fluorescences 3 and arthrobacter sp. 2 strains were isolated from the association of microorganisms--destructors of oil hydrocarbons and were selected for their ability to grow on media with phenanthrene as the only source of carbon and energy. the p. fluorescens 3 strain is able to grow on naphthalene, fluorene, phenanthrene, and anthracene. arthrobacter sp. 2 strain did not grow on naphthalene, but was able to destruct phenanthrene and fluorene. the destruction activity of these st ...200111785266
rates of oxygen and hydrogen exchange as indicators of tpq cofactor orientation in amine oxidases.this study presents the first detailed examination by resonance raman (rr) spectroscopy of the rates of solvent exchange for the c5 and c3 positions of the tpq cofactor in several wild-type copper-containing amine oxidases and mutants of the amine oxidase from hansenula polymorpha (hpao). on the basis of crystal structure analysis and differing rates of c5 [double bond] o and c3 [bond] h exchange within the enzyme systems, but equally rapid rates of c5 [double bond] o and c3 [bond] h exchange in ...200211781110
[biosynthesis and use of coproporphyrins and uroporphyrins and their metal complexes in immune analysis and diagnostic methods].methods of synthesis of coproporphyrin and uroporphyrin by using bacteria of the genus arthrobacter are proposed. metal complexes of coproporphyrin and uroporphyrin with pt, pd, and zn were synthesized. their structures were identified by spectrophotometry, ir spectrometry, 1h-nmr, mass spectrometry, and hplc. data showing the possibility to use coproporphyrin iii-metal complexes as luminophores for fluorescence detection of tumors. the current and prospective uses of metal complexes of water-so ...200111771318
weeds as a source of plant growth promoting rhizobacteria in agricultural soils.the influence of plant growth promoting (pgp) activity of bacterial communities recovered from each of six weed species (barnyard grass (echinochloa crusfalli (l.) beauv.), corn spurrey (spergula arvensis l.), goldenrod (sonchus sp.), italian ryegrass (lolium multiflorum l.), lamb's-quarters (chenopodium album l.), and quack grass (agropyron repens (l.) beauv.)) was examined in relation to the effect it had on the growth of the potato cultivar russet burbank. bacterial species composition and co ...200111766050
[synthesis of anabiosis autoinducers in non-spore-forming bacteria as a mechanism regulating their activity in soil and subsoil sedimentary rocks].non-spore-forming bacteria of the genera arthrobacter and micrococcus, isolated from permafrost subsoil, were found to produce greater amounts of the d1 extracellular factor than closely related collection strains isolated from soil. the effect of this factor, responsible for cell transition to anabiosis, was not species-specific. thus, the d1 crude preparation isolated from the culture liquid of the permafrost isolate arthrobacter globiformis 245 produced an effect on the collection strain arth ...200111763780
a highly efficient transposon mutagenesis system for the tomato pathogen clavibacter michiganensis subsp. michiganensis.a transposon mutagenesis system for clavibacter michiganensis subsp. michiganensis was developed based on antibiotic resistance transposons that were derived from the insertion element is1409 from arthrobacter sp. strain tm1 ncib12013. as a prerequisite, the electroporation efficiency was optimized by using unmethylated dna and treatment of the cells with glycine such that about 5 x 10(6) transformants per microg of dna were generally obtained. electroporation of c. michiganensis subsp. michigan ...200111763129
exposure to airborne microorganisms in fiberboard and chipboard factories.microbiological air sampling was performed in one fiberboard factory and two chipboard factories located in south-eastern poland. it was found that the levels of bacteria, fungi, dust and bacterial endotoxin in the air of examined facilities were high during initial stages of the production cycle (shredding of waste wood, storing of chips) and then sharply decreased during further stages of this cycle (forming and formatting of the boards). in the fiberboard factory, the concentration of airborn ...200111748877
spectroscopic observation of intermediates formed during the oxidative half-reaction of copper/topa quinone-containing phenylethylamine oxidase.the catalytic reaction of copper/topa quinone (tpq) containing amine oxidase consists of the initial, well-characterized, reductive half-reaction and the following, less studied, oxidative half-reaction. we have analyzed the oxidative half-reaction catalyzed by phenylethylamine oxidase from arthrobacter globiformis (agao) by rapid-scan stopped-flow measurements. upon addition of dioxygen to the substrate-reduced agao at ph 8.2, the absorption bands derived from the semiquinone (tpq(sq)) and amin ...200111747456
sugar profiling proves that human serum erythropoietin differs from recombinant human erythropoietin.erythropoietin (epo) from sera obtained from anemic patients was successfully isolated using magnetic beads coated with a human epo (hepo)-specific antibody. human serum epo emerged as a broad band after sodium dodecyl sulfate-polyacrylamide gel electrophoresis, with an apparent molecular weight slightly smaller than that of recombinant hepo (rhepo). the bandwidth corresponded with microheterogeneity because of extensive glycosylation. two-dimensional gel electrophoresis revealing several differ ...200111739166
crystal structure and mechanism of catalysis of a pyrazinamidase from pyrococcus horikoshii.bacterial pyrazinamidase (pzaase)/nicotinamidase converts pyrazinamide (pza) to ammonia and pyrazinoic acid, which is active against mycobacterium tuberculosis. loss of pzaase activity is the major mechanism of pyrazinamide-resistance by m. tuberculosis. we have determined the crystal structure of the gene product of pyrococcus horikoshii 999 (ph999), a pzaase, and its complex with zinc ion by x-ray crystallography. the overall fold of ph999 is similar to that of n-carbamoylsarcosine amidohydrol ...200111714269
cold-active citrate synthase: mutagenesis of active-site residues.a comparison of the crystal structure of the dimeric enzyme citrate synthase from the psychrophilic arthrobacter strain ds2-3r with that of the structurally homologous enzyme from the hyperthermophilic pyrococcus furiosus reveals a significant difference in the accessibility of their active sites to substrates. in this work, we investigated the possible role in cold activity of the greater accessibility of the arthrobacter citrate synthase. by site-directed mutagenesis, we replaced two alanine r ...200111707611
rubrobacter-related bacteria associated with rosy discolouration of masonry and lime wall paintings.a molecular approach was chosen to analyse the correlation between bacterial colonisation and rosy discolouration of masonry and lime wall paintings of two historically important buildings in austria and germany. the applied molecular method included pcr amplification of genes encoding the small subunit rrna of bacteria (16s rdna), genetic fingerprinting by denaturing gradient gel electrophoresis (dgge), construction of 16s rdna clone libraries, and comparative phylogenetic sequence analyses. th ...200111702076
immobilization of the hydantoin cleaving enzymes from arthrobacter aurescens dsm 3747.the immobilization procedure of the two industrially important hydantoin cleaving enzymes--hydantoinase and l-n-carbamoylase from arthrobacter aurescens dsm 3747--was optimized. using different methods (carbodiimide, epoxy activated carriers) it was possible to immobilize the crude hydantoinase from a. aurescens dsm 3747 to supports containing primary amino groups with a yield of up to 60%. immobilization on more hydrophobic supports such as eupergit c and c 250 l resulted in lower yields of act ...200111640987
carotenoid accumulation in the psychrotrophic bacterium arthrobacter agilis in response to thermal and salt stress.a psychrotrophic strain of arthrobacter agilis, isolated from antarctic sea ice, grows from 5 degrees c to 40 degrees c and in culture media containing 0-10% (w/v) nacl. maximum growth rate occurred at 30-35 degrees c with a drastic decline as the cultivation temperatures diverged. adaptation to extremes of low temperature may be partially attributed to the production of the c-50 carotenoid bacterioruberin, and its glycosylated derivatives. lowering of the cultivation temperature resulted in a c ...200111601625
interactions between yeasts and bacteria in the smear surface-ripened cheeses.in the initial phase of ripening, the microflora of bacterial smear surface-ripened cheeses such as limburger, taleggio, brick, münster and saint-paulin and that of surface mould-ripened cheeses such as camembert and brie may be similar, but at the end of the ripening, bacteria such as brevibacterium spp., arthrobacter spp., micrococcus spp., corynebacterium spp. and moulds such as penicillium camemberti are, respectively, the dominant microorganisms. yeasts such as candida spp., cryptococcus sp ...200111589547
bulk and rhizosphere soil bacterial communities studied by denaturing gradient gel electrophoresis: plant-dependent enrichment and seasonal shifts revealed.the bacterial rhizosphere communities of three host plants of the pathogenic fungus verticillium dahliae, field-grown strawberry (fragaria ananassa duch.), oilseed rape (brassica napus l.), and potato (solanum tuberosum l.), were analyzed. we aimed to determine the degree to which the rhizosphere effect is plant dependent and whether this effect would be increased by growing the same crops in two consecutive years. rhizosphere or soil samples were taken five times over the vegetation periods. to ...200111571180
[microbiota of the orchid rhizoplane].six bacterial strains isolated from the underground roots of the terrestrial orchid calanthe vestita var. rubrooculata were found to belong to the genera arthrobacter, bacillus, mycobacterium, and pseudomonas. strains isolated from the aerial roots of the epiphytic orchid dendrobium moschatum were classified into the genera bacillus, curtobacterium, flavobacterium, nocardia, pseudomonas, rhodococcus, and xanthomonas. the rhizoplane of the terrestrial orchid was also populated by cyanobacteria of ...200111558285
[dynamic of the volume and structure of the soil bacterial complex in the presence of azobenzene].azobenzene exerted no significant effect on the dynamics and the species composition of the saprophytic soil bacterial complex, which remained almost the same as in the control and was characterized by the predominance of curtobacterium sp., arthrobacter globiformis, and bacillus megaterium in all stages of succession. some heterotrophic bacteria were found to be able to accumulate azobenzene. bac. cereus and bac. polymyxa degraded azobenzene during their cultivation in nutrient media.200111558283
[formation of microbial populations on the surface of protective coatings].formation of microbial cenosis on the surface of polyethylene-, polyurethane- and oil-bitumen-based protective coatings was studied in dynamics during 1, 3, 7, 14 and 21 days. it has been shown that the biofilm was formed on the protective materials during 14 days and consisted of ammonifying, denitrifying, hydrocarbon-oxidizing and sulphate-reducing bacteria referred to pseudomonas, arthrobacter, bacillus and kesulfovibrio genera. the bacteria which form the biofilm on coatings possess high den ...200111558243
biological potential of extraterrestrial materials. 2. microbial and plant responses to nutrients in the murchison carbonaceous meteorite.meteoritic materials are investigated as potential early planetary nutrients. aqueous extracts of the murchison c2 carbonaceous meteorite are utilized as a sole carbon source by microorganisms, as demonstrated by the genetically modified pseudomonas fluorescence equipped with the lux gene. nutrient effects are observed also with the soil microorganisms nocardia asteroides and arthrobacter pascens that reach populations up to 5 x 10(7) cfu/ml in meteorite extracts, similar to populations in terre ...199711541241
microbial metabolism of tholin.in this paper, we show that a wide variety of common soil bacteria are able to obtain their carbon and energy needs from tholin (a class of complex organic heteropolymers thought to be widely distributed through the solar system; in this case tholin was produced by passage of electrical discharge through a mixture of methane, ammonia, and water vapor). we have isolated aerobic, anaerobic, and facultatively anaerobic bacteria which are able to use tholin as a sole carbon source. organisms which ...199011538367
characterization of 15 selected coccal bacteria isolated from antarctic rock and soil samples from the mcmurdo-dry valleys (south-victoria land).approximately 1500 cultures of microorganisms were isolated from rocks and soils of the ross desert (mcmurdo-dry valleys). from these, 15 coccoid strains were chosen for more detailed investigation. they were characterized by morphological, physiological and chemotaxonomical properties. all isolates were gram-positive, catalase-positive and nonmotile. six strains showed red pigmentation and could be identified as members of the genera micrococcus (m. roseus, m. agilis) or deinococcus. in sp ...198811538335
microbial life at extremely low nutrient levels.many microorganisms ("oligotrophs") grow in distilled water: pseudomonas spp., caulobacter spp., hyphomicrobium spp., arthrobacter spp., seliberia spp., bactoderma alba, corynebacterium spp., amycolata (nocardia) autotrophica, mycobacterium spp., yeasts, and chlorella spp. also, certain lower fungi can be found here. in the laboratory, these organisms thrive on contaminations of the air (co, hydrocarbons, h2, alcohols etc.). all are euryosmotic and often grow also in higher concentrations of ...198611537832
isolation and characterization of psychotrophic bacteria from oil-reservoir water and oil sands.four psychrotrophic strains, which grew at 4 degrees c but not at 37 degrees c, were isolated from japanese oil-reservoir water (strains sib1, sic1, sis1) and canadian oil sands (strain cab1). strains sib1, sis1, and cab1 had a maximum growth rate at 20 degrees c and grew to the highest cell densities at the cultivation temperature of 0-4 degrees c. strain sis1 was capable of growing even at -5 degrees c. the growth profile of strain sic1 was rather similar to that of a mesophilic bacterium. str ...200111525631
cloning, nucleotide sequence and expression of a hydantoinase and carbamoylase gene from arthrobacter aurescens dsm 3745 in escherichia coli and comparison with the corresponding genes from arthrobacter aurescens dsm 3747.the genes encoding hydantoinases (hyuh1) and carbamoylases (hyuc1) from arthrobacter aurescens dsm 3745 and arthrobacter aurescens dsm 3747 (hyuh2, hyuc2) were cloned in escherichia coli and the nucleotide sequences determined. the hydantoinase genes comprised 1,377 base pairs and the carbamoylase genes 1,239 base pairs each. both hydantoinases, as well as both carbamoylases, showed a high degree of nucleotide and amino acid sequence identity (96-98%). the hyuh and hyuc genes were expressed in e ...200111525624
molecular and enzymatic characterization of a levan fructotransferase from microbacterium sp. al-210.microbacterium sp. al-210 producing a novel levan fructotransferase (lftase) was screened from soil samples. the lftase was purified to homogeneity by (nh4)2so4 fractionation, column chromatography on resource q, and superdex 200hr. the molecular weight of the purified enzyme was estimated to be approximately 46 kda by both sds-page and gel filtration, and the enzyme's isoelectric point was ph 4.8. the major product produced from the levan hydrolysis by the enzyme reaction was identified by atmo ...200111522362
gene cluster on pao1 of arthrobacter nicotinovorans involved in degradation of the plant alkaloid nicotine: cloning, purification, and characterization of 2,6-dihydroxypyridine 3-hydroxylase.a 27,690-bp gene cluster involved in the degradation of the plant alkaloid nicotine was characterized from the plasmid pao1 of arthrobacter nicotinovorans. the genes of the heterotrimeric, molybdopterin cofactor (moco)-, flavin adenine dinucleotide (fad)-, and [fe-s] cluster-dependent 6-hydroxypseudooxynicotine (ketone) dehydrogenase (kdh) were identified within this cluster. the gene of the large moco subunit of kdh was located 4,266 bp from the fad and [fe-s] cluster subunit genes. deduced fun ...200111514508
a novel disaccharide substrate having 1,2-oxazoline moiety for detection of transglycosylating activity of endoglycosidases.a disaccharide substrate of manbeta1-4glcnac-oxazoline 2 was designed and synthesized as a novel probe for detection of the transglycosylating activity of endoglycosidases. a regio- and stereoselective transglycosylation reaction of 2 to glcnacbeta1-o-pnp or dns-asn(glcnac)-oh catalyzed by endo-beta-n-acetylglucosaminidase from mucor hiemalis (endo-m) and endo-beta-n-acetylglucosaminidase from arthrobacter protophormiae (endo-a) has been demonstrated for the first time, resulting in the core tri ...200111514092
organization of genes responsible for the stereospecific conversion of hydantoins to alpha-amino acids in arthrobacter aurescens dsm 3747.arthrobacter aurescens dsm 3747 hydrolyzes stereospecifically 5'-monosubstituted hydantoins to alpha-amino acids. the genes involved in hydantoin utilization (hyu) were isolated on an 8.7-kb dna fragment, and by dna sequence analysis eight orfs were identified. the hyu gene cluster includes four genes: hyup encoding a putative transport protein, the hydantoin racemase gene hyua, the hydantoinase gene hyuh, and the carbamoylase gene hyuc. the four genes are transcribed in the same direction. upst ...200111511866
construction, overexpression, and purification of arthrobacter globiformis amine oxidase-strep-tag ii fusion protein.the copper-containing amine oxidase from arthrobacter globiformis has been expressed and purified as a fusion protein with a c-terminal strep-tag ii peptide. this tag facilitates the rapid purification of the enzyme on a large scale using the streptactin poros medium. for example, we have demonstrated that 50 mg of protein can be obtained in 2 days from 2 l of escherichia coli. the purified fusion protein displays turnover and spectroscopic properties that are essentially identical to those of t ...200111483008
alpha 2,3-sialylation of terminal galnac beta 1-3gal determinants by st3gal ii reveals the multifunctionality of the enzyme. the resulting neu5ac alpha 2-3galnac linkage is resistant to sialidases from newcastle disease virus and streptococcus pneumoniae.enzymatic alpha 2,3-sialylation of galnac has not been described previously, although some glycoconjugates containing alpha 2,3-sialylated galnac residues have been reported. in the present experiments, recombinant soluble alpha 2,3-sialyltransferase st3gal ii efficiently sialylated the x(2) pentasaccharide galnac beta 1-3gal beta 1-4glcnac beta 1-3gal beta 1-4glc, globo-n-tetraose galnac beta 1-3gal alpha 1-4gal beta 1-4glc, and the disaccharide galnac beta 1-3gal in vitro. the purified product ...200111479313
modeling of copper biosorption by arthrobacter sp. in a uf/mf membrane reactor.copper biosorption by arthrobacter sp. has been studied in this work. the process has been realized inside of a ultrafiltration/microfiltration (uf/mf) reactor in order to confine cells. a mathematical model has been developed that is able to predict experimental data under different operating conditions. the model takes into account different phenomena, which might occur during the process, such as a dependence of equilibrium parameters on ph, a partial cell disruption, and a change in the memb ...200111478261
trehalose-producing operon treyz from arthrobacter ramosus s34.arthrobacter ramosus s34, which produces trehalose from maltooligosaccharide, was isolated. a trehalose-producing operon, treyz, was cloned from the genome. expression experiments with trey and trez confirmed that they coded malto-oligosyltrehalose synthase and malto-oligosyltrehalose trehalohydrolase, respectively. the amino acid sequence of trey from a. ramosus s34 and that from arthrobacter sp. q36 did not show high identity, nor did those of trez.200111471747
isolation and characterisation of new gram-negative and gram-positive atrazine degrading bacteria from different french soils.the capacity of 12 soils to degrade atrazine was studied in laboratory incubations using radiolabelled atrazine. eight soils showed enhanced degradation of this compound. twenty-five bacterial strains able to degrade atrazine were isolated by an enrichment method from 10 of these soils. these soils were chosen for their wide range of physico-chemical characteristics. their history of treatment with atrazine was also variable. the genetic diversity of atrazine degraders was determined by amplifie ...200111451526
purification and characterization of carbaryl hydrolase from arthrobacter sp. rc100.a carbaryl hydrolase was purified to homogeneity from arthrobacter sp. strain rc100 by protamine sulfate treatment, ammonium sulfate precipitation, and hydrophobic, anion-exchange, and gel filtration chromatographies. the native enzyme had a molecular mass of 100 kda and was composed of two identical subunits with molecular masses of 51 kda. the hydrolase activity was strongly inhibited by difp, pmsf, hg(2+) and paraoxon but not by edta. the optimum ph and temperature for the enzyme activity wer ...200111445174
isolation of poly(3-hydroxybutyrate) (phb)-degrading microorganisms and characterization of phb-depolymerase from arthrobacter sp. strain w6.microbial degraders of poly(3-hydroxybutyrate) (phb) were isolated from soil. arthrobacter sp. strain w6 used not only phb as a carbon source, but also phas such as poly(3-hydroxybutyrate-co-[5%]3-hydroxyvalerate), poly(3-hydroxybutyrate-co-[14%]3-hydroxyvalerate), and poly(3-hydroxybutyrate-co-[22%]3-hydroxyvalerate). phb-depolymerase was purified to homogeneity from the culture broth of arthrobacter sp. strain w6 by a procedure involving deae- and butyl-toyopearl column chromatographies. the m ...200111440137
bioremediation of an area contaminated by a fuel spill.in order to decontaminate a large area of restricted access contaminated by a fuel spill, laboratory and field studies were developed in two steps: (a) monitoring of the laboratory experiment on bacterial growth under aerobic and anaerobic conditions with and without addition of nutrients; and (b) use of the best conditions obtained in (a) for the decontamination of the soil. a hydraulic barrier was installed both to clean the aquifer and to avoid migration of hydrocarbons as a consequence of th ...200111432263
effects of lipids on n-alkane attenuation in media supporting oil-utilizing microorganisms from the oily arabian gulf coasts.the arabian gulf is one of the most extensively oil-polluted areas of the world. the major objectives of this work were to study whether hydrocarbon-utilizing microorganisms indigenous to that area would readily accumulate added lipids, and whether this might affect their hydrocarbon consumption potential. two prokaryotes, arthrobacter nicotianae kcc b35 and the unidentified organisms kcc b6, as well as one eukaryote, candida parapsilosis kcc y1, were selected for this study. biomass samples of ...200111430415
modular structure, local flexibility and cold-activity of a novel chitobiase from a psychrophilic antarctic bacterium.the gene archb encoding for the cell-bound chitobiase from the antarctic gram-positive bacterium arthrobacter sp. tad20 was cloned and expressed in escherichia coli in a soluble form. the mature chitobiase archb possesses four functionally independent domains: a catalytic domain stabilized by ca(2+), a galactose-binding domain and an immunoglobulin-like domain followed by a cell-wall anchorage signal, typical of cell-surface proteins from gram-positive bacteria. binding of saccharides was analyz ...200111428890
dual-bioaugmentation strategy to enhance remediation of cocontaminated soil.although metals are thought to inhibit the ability of microorganisms to degrade organic pollutants, several microbial mechanisms of resistance to metal are known to exist. this study examined the potential of cadmium-resistant microorganisms to reduce soluble cadmium levels to enhance degradation of 2,4-dichlorophenoxyacetic acid (2,4-d) under conditions of cocontamination. four cadmium-resistant soil microorganisms were examined in this study. resistant up to a cadmium concentration of 275 micr ...200111425743
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