| metabolic and functional properties of lactic acid bacteria in the gastro-intestinal ecosystem: a comparative in vitro study between bacteria of intestinal and fermented food origin. | metabolic and functional properties of probiotic lactic acid bacteria (lab) in the human gastro-intestinal ecosystem may be related to certain beneficial health effects. in this study, lactobacilli of either intestinal or fermented food origin were compared in their capability to survive low ph and bile, in their metabolic activity in the presence of bile salts and mucins, as well as in their potential to attach to enterocyte-like caco-2 cells. food fermenting bacteria especially strains of the ... | 2001 | 11518324 |
| [changes in the composition of volatile compounds during processing and storage of jerky sausages]. | the change in the composition of volatile components during aging and storage of jerky sausages containing a mixture of lactobacillus plantarum and staphylococcus carnosus as fermenting cultures was studied by high-performance capillary gas chromatography and gas chromatography-mass spectrometry. a total of 52 compounds were identified. it was found that sausage storage is accompanied by a significant increase in the concentration of flavoring aldehydes. the terpene concentration monotonously in ... | 2001 | 11530674 |
| evidence for dual functionality of the operon plnabcd in the regulation of bacteriocin production in lactobacillus plantarum. | the regulatory operon (plnabcd) involved in bacteriocin production in lactobacillus plantarum c11 encodes four different proteins: a cationic prepeptide (plna); a histidine protein kinase (plnb); and two highly homologous response regulators (plnc and plnd; over 75% sequence similarity). the mature product of plna, plantaricin a, serves as an extracellular pheromone that induces bacteriocin production. the exact roles of plnbcd in bacteriocin production have not been established experimentally. ... | 2001 | 11532131 |
| mode of action, purification and amino acid sequence of plantaricin c19, an anti-listeria bacteriocin produced by lactobacillus plantarum c19. | plantaricin c19, an anti-listeria bacteriocin, was successfully purified by adsorption to and release from producing cells at low ph combined with reverse phase high-performance liquid chromatography (hplc). the purification resulted in a 900-fold increase in specific activity with a yield of 15% of the original activity. mass spectrometry analysis gave a molecular weight of 3845.3. protein microsequencing identified 36 amino acids. plantaricin c19 is rich in both hydrophobic and basic amino aci ... | 2001 | 11545225 |
| evaluation of nitric oxide production by lactobacilli. | six strains of lactobacillus fermentum and lactobacillus plantarum were investigated for nitric oxide (no) production. first, the potential presence of no synthase was examined. none of the strains of l. fermentum and l. plantarum examined produced no from l-arginine under aerobic conditions. interestingly, all l. fermentum strains expressed strong l-arginine deiminase activity. all l. fermentum strains produced no in mrs broth, but the no was found to be chemically derived from nitrite, which w ... | 2001 | 11549028 |
| identification of the translocating bacteria in rats with acute liver injury and their relation to the bacterial flora of the intestinal mucosa. | the bacterial flora of the intestine and the bacteria found in liver, mesenteric lymph nodes, portal and arterial blood after d-galactosamine-induced liver injury, with and without pretreatment with lactobacillus plantarum dsm 9843, were studied in the rat. dominating representatives were identified to species level by 16s rdna sequencing and typed by randomly amplified polymorphic dna (rapd) and by restriction endonuclease analysis (rea) for strain definition. it was proven that bacterial strai ... | 2001 | 11552954 |
| binding and activation of thiamin diphosphate in acetohydroxyacid synthase. | acetohydroxyacid synthases (ahass) are biosynthetic thiamin diphosphate- (thdp) and fad-dependent enzymes. they are homologous to pyruvate oxidase and other members of a family of thdp-dependent enzymes which catalyze reactions in which the first step is decarboxylation of a 2-ketoacid. ahas catalyzes the condensation of the 2-carbon moiety, derived from the decarboxylation of pyruvate, with a second 2-ketoacid, to form acetolactate or acetohydroxybutyrate. a structural model for ahas isozyme ii ... | 2001 | 11570896 |
| crystal structure of manganese catalase from lactobacillus plantarum. | catalases are important antioxidant metalloenzymes that catalyze disproportionation of hydrogen peroxide, forming dioxygen and water. two families of catalases are known, one having a heme cofactor, and the other, a structurally distinct family containing nonheme manganese. we have solved the structure of the mesophilic manganese catalase from lactobacillus plantarum and its azide-inhibited complex. | 2001 | 11587647 |
| in vitro antimicrobial activities of bakuchiol against oral microorganisms. | bakuchiol was isolated from the seeds of psoralea corylifolia, a tree native to china with various uses in traditional medicine, followed by extraction with ether and column chromatography combined with silica gel and octyldecyl silane. in this study, the antimicrobial activities of bakuchiol against some oral microorganisms were evaluated in vitro. the cell growth of streptococcus mutans was inhibited in a bakuchiol concentration-dependent manner, and growth of s. mutans was completely prevente ... | 2001 | 11600349 |
| study of inactivation of lactobacillus plantarum in orange-carrot juice by means of pulsed electric fields: comparison of inactivation kinetics models. | the inactivation kinetics of lactobacillus plantarum was studied in orange-carrot juice using high intensity pulsed electric fields. the results indicated that under the treatment conditions applied, 28.6, 32.0, and 35.8 kv/cm and treatment times ranging from 10.2 to 46.3 micros, the inactivation of l. plantarum obtained was up to 2.5 decimal reductions. experimental and literature data were fitted to bigelow, hülsheger et al. and peleg models and to weibull frequency distribution function. weib ... | 2001 | 11271778 |
| the effect of a dry or liquid application of lactobacillus plantarum mtd1 on the fermentation of alfalfa silage. | alfalfa was wilted to 30 and 54% dry matter and was untreated or treated with lactobacillus plantarum mtd1 applied either as a liquid or dry inoculant to determine whether form of inoculation affected silage fermentation. in silages with 30% dry matter, both forms of inoculation resulted in silages with more lactic acid and a lower ph than in untreated silage after 2 d of ensiling. in addition, both forms of inoculation resulted in silages with lower concentrations of acetic acid between 8 and 4 ... | 2001 | 11699451 |
| a controlled, double-blind, randomized study on the efficacy of lactobacillus plantarum 299v in patients with irritable bowel syndrome. | irritable bowel syndrome (ibs) is a widespread functional disorder of the digestive tract. its aetiology is unknown and therapeutic options are limited. recent reports suggest that probiotics may have a role in regulating the motility of the digestive tract. | 2001 | 11711768 |
| degradation of organochlorine pesticides by meat starter in liquid media and fermented sausage. | the effect of meat starter on the degradation of ddt and lindane was investigated. the insignificant role of lactobacillus plantarum in degrading p,p'-ddt and lindane presented in tryptone soya broth (tsb) and mineral salt medium (msm) with or without 120 ppm nitrite was observed.the degradation of ddt and lindane by micrococcus varians in tsb and msm with or without nitrite were studied. the results indicated that ddt or lindane were degraded during the incubation period. the reduction in ddt a ... | 2002 | 11731034 |
| differential viable count of mixed starter cultures of lactic acid bacteria in doughs by using modified chalmers medium. | the modified chalmers medium appeared as quite suitable for counting a mixed population consisting of different species of lactic acid bacteria used as starter in breadmaking. selected strains of lactobacillus plantarum, leuconostoc mesenteroides subsp. mesenteroides, lactobacillus sanfranciscensis, enterococcus faecalis together with saccharomyces cerevisiae could be easily differentiated and counted with an acceptable recovery in comparison with reference media. | 2001 | 11297368 |
| recombinant lactobacillus plantarum inhibits house dust mite-specific t-cell responses. | recent evidence suggests that chronic exposure to lactobacilli, which are part of the normal intestinal flora, inhibits the development of allergic disorders. allergy is mediated by th2 cells, which produce high levels of il4 and il5, and suppressive effects of lactic acid bacteria on the development of allergy have been attributed to their th1-inducing properties. on the other hand, lactic acid bacteria have also been shown to suppress autoimmune disorders which are mediated by th1 cells produc ... | 2001 | 11678893 |
| molecular characterization of lactobacillus plantarum genes for beta-ketoacyl-acyl carrier protein synthase iii (fabh) and acetyl coenzyme a carboxylase (accbcda), which are essential for fatty acid biosynthesis. | genes for subunits of acetyl coenzyme a carboxylase (acc), which is the enzyme that catalyzes the first step in the synthesis of fatty acids in lactobacillus plantarum l137, were cloned and characterized. we identified six potential open reading frames, namely, manb, fabh, accb, accc, accd, and acca, in that order. nucleotide sequence analysis suggested that fabh encoded beta-ketoacyl-acyl carrier protein synthase iii, that the accb, accc, accd, and acca genes encoded biotin carboxyl carrier pro ... | 2001 | 11133475 |
| isolation and characterization of bacteriophages from fermenting sauerkraut. | this paper presents the first report of bacteriophage isolated from commercial vegetable fermentations. nine phages were isolated from two 90-ton commercial sauerkraut fermentations. these phages were active against fermentation isolates and selected leuconostoc mesenteroides and lactobacillus plantarum strains, including a starter culture. phages were characterized as members of the siphoviridae and myoviridae families. all leuconostoc phages reported previously, primarily of dairy origin, belo ... | 2002 | 11823247 |
| typing and subtyping of 83 clinical isolates purified from surgically implanted silicone feeding tubes by random amplified polymorphic dna amplification. | in this study, 83 clinical isolates purified from biofilms colonizing 18 silicone gastrostomy devices (12 "buttons" and six tubes converted to skin level devices) were selected for subtype characterization utilizing genetic analysis. the tubes, previously used for feeding, remained in place for 3 to 47 months (mean, 20.0 months) in children ranging in age from 6 months to 17 years. classification of specific microbes using random amplified polymorphic dna (rapd) analysis revealed genetic similar ... | 2002 | 11825951 |
| improving artificial neural networks with a pruning methodology and genetic algorithms for their application in microbial growth prediction in food. | the application of artificial neural networks (ann) in predictive microbiology is presented in this paper. this technique was used to build up a predictive model of the joint effect of nacl concentration, ph level and storage temperature on kinetic parameters of the growth curve of lactobacillus plantarum using ann and response surface model (rsm). sigmoid functions were fitted to the data and kinetic parameters were estimated and used to build the models in which the independent variables were ... | 2002 | 11843410 |
| new efficient amylase-producing strains of lactobacillus plantarum and l. fermentum isolated from different nigerian traditional fermented foods. | amylolytic lactic acid bacteria (alab) were isolated from nigerian traditional fermented foods (fufu, burukutu, ogi-baba and kunu-zakki) with the aim of selecting efficient amylase-producing strains. nine isolates were characterized on the basis of their phenotypic and taxo-molecular characteristics. three groups could be distinguished by their fermentation profiles and this was confirmed by dna restriction analysis. though fermentation profiles gave good identification of strain k9 (unique repr ... | 2002 | 11843413 |
| lactobacillus plantarum 299v in the treatment and prevention of spontaneous colitis in interleukin-10-deficient mice. | interleukin (il)-10-deficient (il-10-/-) mice develop colitis under specific pathogen-free (spf) conditions and remain disease free if kept sterile (germ free [gf]). we used four different protocols that varied the time-points of oral administration of lactobacillus plantarum 299v (l. plantarum) relative to colonization with spf bacteria to determine whether l. plantarum could prevent and treat colitis induced by spf bacteria in il-10-/- mice and evaluated the effect of this probiotic organism o ... | 2002 | 11854603 |
| effects of pressure-induced membrane phase transitions on inactivation of hora, an atp-dependent multidrug resistance transporter, in lactobacillus plantarum. | the effects of pressure on cultures of lactobacillus plantarum were characterized by determination of the viability and activity of hora, an atp-binding cassette multidrug resistance transporter. changes in the membrane composition of l. plantarum induced by different growth temperatures were determined. furthermore, the effect of the growth temperature of a culture on pressure inactivation at 200 mpa was determined. cells were characterized by plate counts on selective and nonselective agar aft ... | 2002 | 11872454 |
| differentiation of lactobacillus plantarum, l. pentosus and l. paraplantarum species by rapd-pcr and aflp. | two high-resolution genotypic techniques (rapd-pcr and aflp) were evaluated for their possibility to discriminate the species lactobacillus plantarum, lactobacillus pentosus and lactobacillus paraplantarum and to type these taxa at the infra-species level. in total 23 strains of l. plantarum, three strains of l. pentosus, two strains of l. paraplantarum and two related strains for which the species assignment was not clear, were studied. for rapd-pcr, suitable oligonucleotides and amplification ... | 2001 | 11876363 |
| polyphasic investigation of the diversity within lactobacillus plantarum related strains revealed two l. plantarum subgroups. | the diversity of 140 strains related to lactobacillus plantarum was investigated using a polyphasic approach combining two molecular techniques: randomly amplified polymorphic dna fingerprinting (rapd) and southern hybridisation with a pyr probe on bgli digests of chromosomal dna, as well as phenotypic characterization. the rapd technique allowed us to classify a subset of 60 representative strains into four groups. one group belonged to lactobacillus paraplantarum, the second to lactobacillus p ... | 2001 | 11876364 |
| effect of inoculants on whole-crop barley silage fermentation and dry matter disappearance in situ. | whole-crop barley harvested at the mid-dough stage was ensiled in 3-l laboratory silos either directly (at 30.7% dm) or after wilting (37.8% dm), and with or without application of inoculant a, b, or d. each inoculant contained multiple strains of lactobacillus plantarum and enterococcus faecium. two silos per treatment were opened on d 1, 3, 7, 15, and 47 for silage analysis. wilted silages had higher (p < 0.05) ph than unwilted silages, and inoculant b (unwilted crop) and inoculants a, b, and ... | 2002 | 11881935 |
| comparison of the immune responses induced by local immunizations with recombinant lactobacillus plantarum producing tetanus toxin fragment c in different cellular locations. | lactobacillus plantarum ncimb8826 was selected as a bacterial carrier for the development of live mucosal vaccines. this strain was reported to display interesting pharmaco-kinetic properties when fed to human volunteers and is also able to persist in the mouse intestine. the non-toxic c fragment of tetanus toxin (ttfc) was used as a model antigen. recombinant strains producing ttfc in three cellular locations, intracellular, secreted or cell-surface exposed were compared to each other by immuni ... | 2002 | 11906764 |
| lactobacillus plantarum 299v inhibits escherichia coli-induced intestinal permeability. | the purpose of this work was to investigate whether a probiotic bacterium, lactobacillus plantarum 299v, could affect escherichia coli-induced passage of mannitol across the intestinal wall. sprague-dawley rats were pretreated for one week by either tube feeding with l. plantarum 299v twice daily, free access to l. plantarum 299v by adding the bacterium in the drinking water, or negative control receiving regular feeding. intestinal segments were mounted in ussing chambers and the mucosa was exp ... | 2002 | 11911334 |
| osmosensing and osmoregulatory compatible solute accumulation by bacteria. | bacteria inhabit natural and artificial environments with diverse and fluctuating osmolalities, salinities and temperatures. many maintain cytoplasmic hydration, growth and survival most effectively by accumulating kosmotropic organic solutes (compatible solutes) when medium osmolality is high or temperature is low (above freezing). they release these solutes into their environment when the medium osmolality drops. solutes accumulate either by synthesis or by transport from the extracellular med ... | 2001 | 11913457 |
| ruminal supplementation of direct-fed microbials on diurnal ph variation and in situ digestion in dairy cattle. | to evaluate the effect of direct-fed microbial (dfm) concentration on diurnal rumen ph profiles and in situ digestibilities, nine ruminally cannulated cows in early lactation were fed treatments consisting of dfm (enterococcus faecium, lactobacillus plantarum, and saccharomyces cerevisiae) at a level of a) 1 x 10(5) cfu/ml of rumen fluid (10(5)), b) 1 x 10(6) cfu/ml of rumen fluid (10(6)), and c) 1 x 10(7) cfu/ml rumen fluid (10(7)). treatments were directly administered via rumen cannula once d ... | 2002 | 11915864 |
| chemical and microbiological characterisation of kefir grains. | chemical and microbiological composition of four argentinean kefir grains from different sources as well as characteristics of the corresponding fermented milk were studied. kefir grains cidca agk1, agk2 and agk4 did not show significant differences in their chemical and microbiological composition. in contrast, protein and yeast content of agk3 was higher than in the other grains. although grain microflora comprised lactobacilli, lactococcus, acetic acid bacteria and yeast, we found an importan ... | 2001 | 11928960 |
| antibacterial activity of chitosans and chitosan oligomers with different molecular weights. | antibacterial activities of six chitosans and six chitosan oligomers with different molecular weights (mws) were examined against four gram-negative (escherichia coli, pseudomonas fluorescens, salmonella typhimurium, and vibrio parahaemolyticus) and seven gram-positive bacteria (listeria monocytogenes, bacillus megaterium, b. cereus, staphylococcus aureus, lactobacillus plantarum, l. brevis, and l. bulgaricus). chitosans showed higher antibacterial activities than chitosan oligomers and markedly ... | 2002 | 11929171 |
| vector-free cloning of a bacterial endo-1,4-beta-glucanase in lactobacillus plantarum and its effect on the acidifying activity in silage: use of recombinant cellulolytic lactobacillus plantarum as silage inoculant. | in this research, the advantage of use of cellulolytic recombinant lactobacillus plantarum as microbial inoculants for alfalfa silage fermentation was evaluated. to such purpose, two l. plantarum strains, one (l. plantarum lp80) currently commercialised and the other (l. plantarum b41) suitable as silage microbial additive, were genetically modified by integration of cela gene, encoding an alkaline endo-1,4-beta-glucanase from bacillus sp., in the chromosome, by means of a vector-free cloning te ... | 2001 | 11759047 |
| antimicrobial characteristics of chitosans against food spoilage microorganisms in liquid media and mayonnaise. | four different kinds of chitosans were prepared by treating crude chitin with various naoh concentrations. the antimicrobial activities of the chitosans were tested against four species of food spoilage microorganisms (lactobacillus plantarum, lactobacillus fructivorans, serratia liquefaciens, and zygosaccharomyces bailii). the initial effect of the chitosans was biocidal, and counts of viable cells were significantly reduced. after an extended lag phase, some strains recovered and resumed growt ... | 2001 | 11791708 |
| mutational analysis of the bglh catabolite-responsive element (cre) in lactobacillus plantarum. | catabolite repression of the lactobacillus plantarum bglh gene is mediated by the catabolite control protein a (ccpa). here we show that the binding site for the protein is a catabolite-responsive element (cre) sequence overlapping the start site of transcription. two single and one double base substitutions in the cre sequence caused derepression of a plasmid-borne bglh-cat transcriptional fusion in l. plantarum cells grown on glucose. analysis of the single mutations indicates that the g and c ... | 2002 | 11934508 |
| ensiling whole-crop wheat and corn in large containers with lactobacillus plantarum and lactobacillus buchneri. | the effect of applying lactobacillus buchneri, alone or in combination with lactobacillus plantarum, at ensiling, on the aerobic stability of wheat and corn silages was studied in 50-l plastic containers. treatments comprised control (no additives), l. plantarum, l. buchneri and a combination of l. plantarum+l. buchneri. after 3 months of storage, the wheat silages treated with l. buchneri had higher acetic acid contents than the control or l. plantarum-treated silages, and were free of mold, wh ... | 2002 | 11938464 |
| magnetization studies of the active and fluoride-inhibited derivatives of the reduced catalase of lactobacillus plantarum: toward a general picture of the anion-inhibited and active forms of the reduced dimanganese catalases. | the magnetic properties of the reduced catalase from lactobacillus plantarum have been studied for the active enzyme and its fluoride complex through variable field/variable temperature magnetization measurements. the magnetic exchange interaction deduced from these experiments [fluoride complex: - j=1.3(1) cm(-1); active enzyme: - j=5.6(5) cm(-1); h=-2 j s(1) s(2)] are similar to those presently obtained in a re-analysis of the data for the corresponding forms of the thermus thermophilus enzyme ... | 2002 | 11941502 |
| isolation and characterization of lactic acid bacteria from feces of newborn baby and from dongchimi. | lactic acid bacteria were screened from feces of newborn baby and from dongchimi. selection criteria employed included the ability of strains to withstand environmental conditions such as low ph, high bile concentration, and oxygen. the isolates were applied to the juice of various vegetables, and fermentabilities of isolates were compared. strains f20-3, f35-3, and f35-6 showed high stability compared to the other strains at ph 3.0 and 2.3. strains d1 and d2 showed the highest survival at ph 3. ... | 2002 | 11958617 |
| growth studies of potentially probiotic lactic acid bacteria in cereal-based substrates. | the overall growth kinetics of four potentially probiotic strains (lactobacillus fermentum, lact. reuteri, lact. acidophilus and lact. plantarum) cultured in malt, barley and wheat media were investigated. the objectives were to identify the main factors influencing the growth and metabolic activity of each strain in association with the cereal substrate. | 2002 | 11972688 |
| efficient protoplast regeneration for some homofermentative lactobacilli and pediococci. | conditions for protoplast regeneration were examined for several strains of homofermentative lactobacilli and pediococci isolated from silage. attempts to regenerate protoplasts using previously published agar regeneration media for lactobacilli were unsuccessful for most of the strains. replacing or increasing colloidal substances in a medium containing raffinose and mgcl(2) as osmotic stabilizers enabled efficient regeneration of the protoplasts at a frequency of 10-99%. a medium containing ge ... | 2001 | 11797042 |
| modelling the effect of sublethal injury on the distribution of the lag times of individual cells of lactobacillus plantarum. | the effect of heat stress on subsequent duration of the lag time of individual cells of lactobacillus plantarum was analysed by flow cytometry. the results show clearly that both the mean and the standard deviation of the distribution of the lag time increased after sublethal heat treatment. the distributions of the lag times or the log lag times of untreated and treated cells, respectively, could be described as extreme value distributions. from these distributions, the distribution of the mini ... | 2002 | 11934029 |
| characterization of the genetic locus responsible for the production of abp-118, a novel bacteriocin produced by the probiotic bacterium lactobacillus salivarius subsp. salivarius ucc118. | abp-118, a small heat-stable bacteriocin produced by lactobacillus salivarius subsp. salivarius ucc118, a strain isolated from the ileal-caecal region of the human gastrointestinal tract, was purified to homogeneity. using reverse genetics, a dna fragment specifying part of abp-118 was identified on a 10769 bp chromosomal region. analysis of this region revealed that abp-118 was a class iib two-peptide bacteriocin composed of abp118alpha, which exhibited the antimicrobial activity, and abp118bet ... | 2002 | 11932444 |
| effect of fermented feed on shedding of enterobacteriaceae by fattening pigs. | epidemiological studies showed that the use of fermented feed could significantly reduce salmonella prevalence in pigs compared to the use of normal feed. experimental challenge experiments with salmonella have however never been conducted to reveal the efficacy of fermented feed in reducing salmonella shedding and/or reducing the number of salmonella-positive pigs. a longitudinal study was conducted to measure the effect of fermented feed, in particular of its components lactic acid and lactoba ... | 2002 | 12052336 |
| irritable bowel syndrome. | because treatment of irritable bowel syndrome (ibs) patients can be frustrating to the clinician and patient as well, the physician should strive to gain the patient's confidence with a concise, appropriate work-up and by offering reassurance and education that ibs is a functional disorder without significant long-term health risks. first-line treatment should be aimed at treating the most bothersome symptom. tricyclic antidepressants are superior to placebo in reducing abdominal pain scores, as ... | 2002 | 12095474 |
| early enteral supply of lactobacillus and fiber versus selective bowel decontamination: a controlled trial in liver transplant recipients. | early enteral nutrition with solutions containing prebiotics (fiber) and probiotics (lactobacillus) is suggested to reduce bacterial translocation and minimize the incidence of infections after liver transplantation. | 2002 | 12134110 |
| effects of pulsed electric fields on inactivation and metabolic activity of lactobacillus plantarum in model beer. | inactivation and sublethal injury of lactobacillus plantarum at different pulsed electric field (pef) strengths and total energy inputs were investigated to differentiate reversible and irreversible impacts on cell functionality. | 2002 | 12147082 |
| biochemical and genetic characterization of mundticin ks, an antilisterial peptide produced by enterococcus mundtii nfri 7393. | mundticin ks, a bacteriocin produced by enterococcus mundtii nfri 7393 isolated from grass silage in thailand, is active against closely related lactic acid bacteria and the food-borne pathogen listeria monocytogenes. in this study, biochemical and genetic characterization of mundticin ks was done. mundticin ks was purified to homogeneity by ammonium sulfate precipitation, sequential ion-exchange chromatography, and solid-phase extraction. the gene cluster (mun locus) for mundticin ks production ... | 2002 | 12147478 |
| bacterial inactivation by high-pressure homogenisation and high hydrostatic pressure. | the resistance of five gram-positive bacteria, enterococcus faecalis, staphylococcus aureus, lactobacillus plantarum, listeria innocua and leuconostoc dextranicum, and six gram-negative bacteria, salmonella enterica serovar typhimurium, shigella flexneri, yersinia enterocolitica, pseudomonas fluorescens and two strains of escherichia coli, to high-pressure homogenisation (100-300 mpa) and to high hydrostatic pressure (200-400 mpa) was compared in this study. within the group of gram-positive bac ... | 2002 | 12160080 |
| heat resistance of juice spoilage microorganisms. | the heat resistance of various yeasts (saccharomyces cerevisiae, rhodotorula mucilaginosa, torulaspora delbrueckii, and zygosaccharomyces rouxii), molds (penicillium citrinum, penicillium roquefortii, and aspergillus niger), and lactic acid bacteria (lactobacillus fermentum and lactobacillus plantarum) obtained from spoiled acid or acidified food products was determined in 0.1 m citrate buffer at ph values of 3.0, 3.5, and 4.0. s. cerevisiae was the most heat resistant of the microorganisms in c ... | 2002 | 12182479 |
| characterization of the major tail protein gpp encoded by lactobacillus plantarum phage phi gle. | lactobacillus plantarum temperate phage phi g1e encodes a major virion protein gpp. in the present study, the gpp protein was overproduced in escherichia coli under plac, and purified. like the native-gpp protein from phi gle particles (kakikawa et al., 1996), the purified-gpp protein had an apparent molecular mass of 26.0 kda on sds polyacrylamide gel electrophoresis (page), larger than that (18.8 kda) predicted from the dna sequence, and was deficient in the first methionine as revealed by the ... | 2002 | 12186753 |
| [searching for restriction endonucleases, finding out the heterogeneity of the 16s rrna in some species of lactic acid bacteria]. | the preliminary test of carbohydrates fermentation has been carried out to confirm a regular position for some lactic acid bacteria strains. restriction cards have been compiled for lactobacillus plantarum, l. delbrueckii and streptococcus salivarius using vector nti program. a comparative analysis of the restriction cards has shown presence of unique sites of restriction endonucleases, which can be used for differentiation of the above-stated species. | 2002 | 12190020 |
| randomized clinical trial of specific lactobacillus and fibre supplement to early enteral nutrition in patients with acute pancreatitis. | microbial infection of the pancreatic tissue in patients with severe acute pancreatitis increases the morbidity and mortality rates. colonization of the lower gastrointestinal tract and oropharynx with gram-negative, but sometimes also gram-positive, bacteria precedes contamination of the pancreas. the aim of this study was to determine whether lactic acid bacteria such as lactobacillus plantarum 299 could prevent colonization of the gut by potential pathogens and thus reduce the endotoxaemia as ... | 2002 | 12190674 |
| tnf-alpha sensitizes ht-29 colonic epithelial cells to intestinal lactobacilli. | the ability of the proinflammatory cytokine tumor necrosis factor-alpha (tnf-alpha) to influence epithelial interleukin (il)-8 responses to the intestinal bacterium lactobacillus plantarum 299v was analyzed in the human ht-29 colonic epithelial cell line. in the absence of tnf-alpha, il-8 mrna expression was not detectable by northern blot analysis in ht-29 cells alone or in ht-29 cells co-cultured with l. plantarum 299v. however, tnf-alpha induced il-8 mrna expression, and co-culture of tnf-alp ... | 2002 | 12192111 |
| lactobacillus plantarum milab 393 produces the antifungal cyclic dipeptides cyclo(l-phe-l-pro) and cyclo(l-phe-trans-4-oh-l-pro) and 3-phenyllactic acid. | we have isolated a lactobacillus plantarum strain (milab 393) from grass silage that produces broad-spectrum antifungal compounds, active against food- and feed-borne filamentous fungi and yeasts in a dual-culture agar plate assay. fusarium sporotrichioides and aspergillus fumigatus were the most sensitive among the molds, and kluyveromyces marxianus was the most sensitive yeast species. no inhibitory activity could be detected against the mold penicillium roqueforti or the yeast zygosaccharomyc ... | 2002 | 12200282 |
| in situ determination of the intracellular ph of lactococcus lactis and lactobacillus plantarum during pressure treatment. | hydrostatic pressure may affect the intracellular ph of microorganisms by (i) enhancing the dissociation of weak organic acids and (ii) increasing the permeability of the cytoplasmic membrane and inactivation of enzymes required for ph homeostasis. the internal phs of lactococcus lactis and lactobacillus plantarum during and after pressure treatment at 200 and 300 mpa and at ph values ranging from 4.0 to 6.5 were determined. pressure treatment at 200 mpa for up to 20 min did not reduce the viabi ... | 2002 | 12200293 |
| optimization of bacteriocin production by batch fermentation of lactobacillus plantarum lpco10. | optimization of bacteriocin production by lactobacillus plantarum lpco10 was explored by an integral statistical approach. in a prospective series of experiments, glucose and nacl concentrations in the culture medium, inoculum size, aeration of the culture, and growth temperature were statistically combined using an experimental 2(3)(5-2) fractional factorial two-level design and tested for their influence on maximal bacteriocin production by l. plantarum lpco10. after the values for the less-in ... | 2002 | 12200301 |
| protection against tetanus toxin after intragastric administration of two recombinant lactic acid bacteria: impact of strain viability and in vivo persistence. | non-pathogenic lactic acid bacteria (lab) are attractive as live carriers to deliver protective antigens to the mucosal immune system. both persisting and non-persisting strains of lactic acid bacteria have been evaluated and seem to work equally well by the systemic and nasal routes of administration. however, it is not known if persistence and viability of the strain play a critical role when immunizing by the oral route. to address this question, recombinant lab strains, able to persist (lact ... | 2002 | 12213400 |
| cold shock induction of the cspl gene in lactobacillus plantarum involves transcriptional regulation. | fragments of the cspl promoter region were fused to the gusa reporter and reintroduced into lactobacillus plantarum cells, either on multicopy plasmids or through single-copy chromosomal integration. beta-glucuronidase activity and primer extension data demonstrate that the cspl promoter is induced in response to cold shock and that multicopy constructs quench the induction of the resident cspl gene. | 2002 | 12218042 |
| utilization of lactobacillus sp. for steroid glycoalkaloids degradation by lactic acid fermentation. | the degradation fo steroid glycoalkaloids (sgas) has been studied in model solutions. the number of colony forming units (cfu) was determined using a nondirect (cultivation) method during all stages of fermentation. the changes in sgas content were observed by hplc on the supelcosil lc-nh2 column. the changes in alpha-tomatine concentration added to fermented lactobacillus mrs broth have been studied. a mathematical model of steroid glycoalkaloids degradation during lactic fermentation was propo ... | 2002 | 12224420 |
| the evaluation of mixtures of yeast and potato extracts in growth media for biomass production of lactic cultures. | the effectiveness of yeast extracts (ye) and potato extracts (pe) to promote growth of seven lactic cultures was evaluated by automated spectrophotometry (as). two aspects of the growth curve were analysed: (1) maximum biomass obtained (using odmax) and (2) highest specific growth rate mu(max)) eleven lots from the same pe-manufacturing process were examined for lot-to-lot variability. the odmax values of three of the seven strains were significantly affected by lot source, but mu(max) was not s ... | 2002 | 12224561 |
| antifungal activities of two lactobacillus plantarum strains against fusarium moulds in vitro and in malting of barley. | the lactobacillus plantarum strains vtt e-78076 (e76) and vtt e-79098 (e98) were studied for their antifungal potential against fusarium species. | 2002 | 12234339 |
| effects of oxidoreduction potential combined with acetic acid, nacl and temperature on the growth, acidification, and membrane properties of lactobacillus plantarum. | the effects of oxidoreduction potential (eh) combined with acetic acid, nacl and temperature on the growth, acidification, and membrane properties of lactobacillus plantarum were studied. the culture medium was set at ph 5, and two different eh values were adjusted using nitrogen (eh = +350 mv) or hydrogen (eh = -300 mv) gas. in reducing condition, the growth was slowed and the acidification delayed at 37 degrees c, but not at 10 degrees c. a synergistic inhibitory effect of reducing eh, acetic ... | 2002 | 12351240 |
| lactobacillus plantarum reduces the in vitro secretory response of intestinal epithelial cells to enteropathogenic escherichia coli infection. | enteropathogenic (epec) is a gram-negative bacillus that causes diarrhea. secretory responses of intestinal epithelial cells can be seen after epec infection. probiotics, which are live bacteria that have proven benefit to the host, play a role in the treatment and prevention of the different enteric pathogens. the goals of the study were to determine whether the probiotic agent (lbp) strain 299v alters the secretory changes seen in epec infection and, if so, what underlying mechanism is possibl ... | 2002 | 12352526 |
| preparation of highly efficient manganese catalase mimics. | the series of compounds [mn(bpia)(mu-oac)](2)(clo(4))(2) (1), [mn(2)(bpia)(2)(muo)(mu-oac)](clo(4))(3).ch(3)cn (2), [mn(bpia)(mu-o)](2)(clo(4))(2)(pf(6)).2ch(3)cn (3), [mn(bpia)(cl)(2)](clo)(4) (4), and [(mn(bpia)(cl))(2)(mu-o)](clo(4))(2).2ch(3)cn (5) (bpia = bis(picolyl)(n-methylimidazol-2-yl)amine) represents a structural, spectroscopic, and functional model system for manganese catalases. compounds 3 and 5 have been synthesized from 2 via bulk electrolysis and ligand exchange, respectively. ... | 2002 | 12377052 |
| behavior of lactobacillus plantarum and saccharomyces cerevisiae in fresh and thermally processed orange juice. | lactobacillus plantarum and saccharomyces cerevisiae are acid-tolerant microorganisms that are able to spoil citrus juices before and after pasteurization. the growth of these microorganisms in orange juice with and without pasteurization was investigated. two samples of orange juice were inoculated with ca. 10(5) cfu/ml of each microorganism. others were inoculated with ca. 10(7) cfu/ml of each microorganism and then thermally treated. l. plantarum populations were reduced by 2.5 and <1 log10 c ... | 2002 | 12380743 |
| comparative survey in lactobacillus plantarum of the growth and metabolism of arginine and citrulline in different media. | arginine deiminase activity increased in the presence of arginine in lactobacillus plantarum strains n4 and n8 isolated from orange. the influence of citrulline and ornithine on arginine deiminase and ornithine transcarbamylase activities was strain-dependent. the growth and arginine and citrulline metabolism of l. plantarum were studied in the presence of tomato juice. its addition enhances the growth in both strains. the specific amino acids utilization was inversely proportional to the initia ... | 2002 | 12381139 |
| the effect of fermented liquid feeding on the faecal microbiology and colostrum quality of farrowing sows. | this study investigated the effects of fermented liquid feed (flf) on the lactic acid bacteria (lab):coliform (l:c) ratio in the faeces of farrowing sows and the quality of sow colostrum. eighteen multiparous sows were randomly allocated to one of three dietary treatments for approximately 2 weeks prior to farrowing and for 3 weeks after parturition. the three dietary treatments were dry pelleted feed (dpf), nonfermented liquid feed (nflf), and fermented liquid feed (flf). a rifampicin-resistant ... | 2002 | 12382688 |
| characterization of the tetracycline resistance plasmid pmd5057 from lactobacillus plantarum 5057 reveals a composite structure. | the 10,877bp tetracycline resistance plasmid pmd5057 from lactobacillus plantarum 5057 was completely sequenced. the sequence revealed a composite structure containing dna from up to four different sources. the replication region had homology to other plasmids of lactic acid bacteria while the tetracycline resistance region, containing a tet(m) gene, had high homology to sequences from clostridium perfringens and staphylococcus aureus. within the tetracycline resistance region a lactobacillus is ... | 2002 | 12383727 |
| characterization of lactobacillus plantarum from wine must by pcr species-specific and rapd-pcr. | physiological and molecular analysis such as pcr species-specific and randomly amplified polymorphic pcr (rapd-pcr) have been used for typing of lactobacillus plantarum strains from typical wine must. | 2002 | 12390483 |
| evaluation of the heat inactivation of escherichia coli and lactobacillus plantarum by differential scanning calorimetry. | differential scanning calorimetry (dsc) is used to evaluate the thermal stability and reversibility after heat treatment of transitions associated with various cellular components of escherichia coli and lactobacillus plantarum. the reversibility and the change in the thermal stability of individual transitions are evaluated by a second temperature scan after preheating in the dsc to various temperatures between 40 and 130 degrees c. the viability of bacteria after a heat treatment between 55 an ... | 2002 | 12406728 |
| characterization of the mela locus for alpha-galactosidase in lactobacillus plantarum. | alpha-galactosides are abundant sugars in legumes such as soy. because of the lack of alpha-galactosidase (alpha-gal) in the digestive tract, humans are unable to digest these sugars, which consequently induce flatulence. to develop the consumption of the otherwise highly nutritional soy products, the use of exogenous alpha-gal is promising. in this framework, we characterized the mela gene for alpha-gal in lactobacillus plantarum. the mela gene encodes a cytoplasmic 84-kda protein whose enzymat ... | 2002 | 12406739 |
| use of the alr gene as a food-grade selection marker in lactic acid bacteria. | both lactococcus lactis and lactobacillus plantarum contain a single alr gene, encoding an alanine racemase (ec 5.1.1.1), which catalyzes the interconversion of d-alanine and l-alanine. the alr genes of these lactic acid bacteria were investigated for their application as food-grade selection markers in a heterologous complementation approach. since isogenic mutants of both species carrying an alr deletion (deltaalr) showed auxotrophy for d-alanine, plasmids carrying a heterologous alr were cons ... | 2002 | 12406763 |
| characterization and heterologous expression of a class iia bacteriocin, plantaricin 423 from lactobacillus plantarum 423, in saccharomyces cerevisiae. | lactobacillus plantarum 423 produces a small heat-stable antimicrobial protein designated plantaricin 423. this protein is bactericidal for many gram-positive foodborne pathogens and spoilage bacteria, including listeria spp., staphylococcus spp., pediococcus spp., lactobacillus spp., etc. the dna sequence of the plantaricin 423-encoding region on plasmid ppla4 revealed a four open reading frame (orf) operon structure similar to pediocin pa-1/ach from pediococcus acidilactici and coagulin from b ... | 2003 | 12423916 |
| microbiological and fermentation characteristics of togwa, a tanzanian fermented food. | selected microbiological and metabolic characteristics of sorghum, maize, millet and maize-sorghum togwa were investigated during natural fermentation for 24 h. the process was predominated by lactic acid bacteria (lab) and yeasts. the mesophiles, lactic acid bacteria, and yeasts increased and the enterobacteriaceae decreased to undetectable levels within 24 h. the isolated microorganisms were tentatively identified as lactobacillus plantarum, lactobacillus brevis, lactobacillus fermentum, lacto ... | 2003 | 12423921 |
| characterization of a highly thermostable extracellular lipase from lactobacillus plantarum. | after screening for the presence of lipase activity in lactobacilli isolated from "chouriço", a traditional portuguese dry fermented sausage, a strain of lactobacillus plantarum (dsmz 12028) was chosen for extracellular lipase characterisation and purification. proteinase k did not significantly affect lipolytic activity, as opposed to trypsin, which completely eliminated this activity. among nacl, ca2+, edta, bsa, glycerol, mn2+ and mg2+, only mn2+ and mg2+ stimulated the lipase. purification b ... | 2002 | 12038566 |
| [influence of probiotics and fibre on the incidence of bacterial infections following major abdominal surgery - results of a prospective trial]. | early enteral nutrition with fibre and probiotics has been effective in preventing bacterial translocation and is therefore expected to reduce the incidence of postoperative bacterial infections. | 2002 | 12436353 |
| development of a flow cytometric method to analyze subpopulations of bacteria in probiotic products and dairy starters. | flow cytometry (fcm) is a rapid and sensitive technique that can determine cell numbers and measure various physiological characteristics of individual cells by using appropriate fluorescent probes. previously, we developed an fcm assay with the viability probes carboxyfluorescein diacetate (cfda) and toto-1 [1'-(4,4,7,7-tetramethyl-4,7-diazaundecamethylene)-bis-4-[3-methyl-2,3dihydro(benzo-1,3-oxazole)-2-methylidene]-1-(3'-trimethylammoniumpropyl)-pyridinium tetraiodide] for (stressed) lactic a ... | 2002 | 12039752 |
| the locus responsible for production of plantaricin s, a class iib bacteriocin produced by lactobacillus plantarum lpco10, is widely distributed among wild-type lact. plantarum strains isolated from olive fermentations. | the genes plsa and plsb encoding for production of plantaricin s (pls), a two-peptide bacteriocin produced by lactobacillus plantarum lpco10, are commonly distributed among wild-type lact. plantarum strains isolated from olive fermentations. among 68 independent isolates from different olive processing plants in south spain, 15 of them were shown to produce bacteriocins that were active against other lactic acid bacteria, as well as spoilage and pathogenic bacteria. on the basis of pcr amplifica ... | 2002 | 12076029 |
| a prospective randomised study of the probiotic lactobacillus plantarum 299v on indices of gut barrier function in elective surgical patients. | bacterial translocation occurs in surgical patients and may predispose to postoperative septic morbidity. many factors are thought to influence the prevalence of bacterial translocation, one of which is the composition of the gut microflora. the aim of this prospective and randomised study was to assess the effect of the probiotic lactobacillus plantarum 299v on the incidence of bacterial translocation, gastric colonisation, and septic complications in elective surgical patients. | 2002 | 12427785 |
| effect of lactobacillus plantarum 299v on cardiovascular disease risk factors in smokers. | the short-chain fatty acids formed in the human colon by the bacterial fermentation of fiber may have an antiinflammatory effect, may reduce insulin production, and may improve lipid metabolism. we previously showed in hypercholesterolemic patients that supplementation with the probiotic bacteria lactobacillus plantarum 299v significantly lowers concentrations of ldl cholesterol and fibrinogen. | 2002 | 12450890 |
| innate immune responses of human neonatal cells to bacteria from the normal gastrointestinal flora. | the hygiene hypothesis postulates that the prevalence of allergy has increased due to decreased microbial stimulation early in life, leading to delayed maturation of the immune system. the aim of this study was to examine the cytokine pattern produced from cord blood mononuclear cells relative to adult cells after stimulation with bacterial strains from the normal flora. mononuclear cells from cord and adult blood samples were stimulated with the following bacteria: bifidobacterium adolescentis, ... | 2002 | 12438343 |
| ricinoleic acid and castor oil as substrates for conjugated linoleic acid production by washed cells of lactobacillus plantarum. | ricinoleic acid (12-hydroxy-cis-9-octadecaenoic acid) was an effective substrate for conjugated linoleic acid (cla) production by washed cells of lactobacillus plantarum aku 1009a. the cla produced was a mixture of cis-9,trans-11- and trans-9,trans-11-octadecadienoic acids. addition of alpha-linolenic acid to the culture medium increased the cla productivity of the washed cells. in the presence of lipase, castor oil, in which the main fatty acid component is ricinoleic acid, also was a substrate ... | 2002 | 12450151 |
| molecular analysis of the lysis protein lys encoded by lactobacillus plantarum phage phig1e. | the putative cell-lysis gene lys of lactobacillus plantarum g1e phage phig1e encodes for a 442 amino-acids protein lys. the n-terminal region (about 80 amino acids) of lys consists of two discrete regions (the signal-peptide-like domain and the de domain containing putative active sites of endolysin). to elucidate functions of the regions of lys, mutational (random, site-directed, and/or fusion) analysis was performed. the plasmid pndehl, expressing the wild type lys protein under promoter of la ... | 2002 | 12459270 |
| the effect of sodium acetate on the growth yield, the production of l- and d-lactic acid, and the activity of some enzymes of the glycolytic pathway of lactobacillus sakei nric 1071(t) and lactobacillus plantarum nric 1067(t). | the effect of sodium acetate was studied on the change of the growth yield, the production of l- and d-lactic acid, and the activity of lactate dehydrogenases (ldhs; l-lactate dehydrogenase [ec 1.1.1.27, l-ldh] plus d-lactate dehydrogenase [ec 1.1.1.28, d-ldh]), fructose-1, 6-bisphosphate aldolase [ec 4.1.2.13, fbp-aldolase], and phosphofructokinase [ec 2.7.1.11, pfk] of lactobacillus sakei nric 1071(t) and lactobacillus plantarum nric 1067(t). the growth yield of l. sakei nric 1071(t) was incre ... | 2002 | 12469305 |
| lactic acid bacteria isolated from soy sauce mash in thailand. | fourteen sphere-shaped and 30 rod-shaped lactic acid bacteria were isolated from soy sauce mash of two factories in thailand. these strains were separated into two groups, group a and group b, by cell shape and dna-dna similarity. group a contained 14 tetrad-forming strains, and these strains were identified as tetragenococcus halophilus by dna similarity. group b contained 30 rod-shaped bacteria, and they were further divided into four subgroups, b1, b2, b3, and b4, and three ungrouped strains ... | 2002 | 12469319 |
| the lactoperoxidase system increases efficacy of high-pressure inactivation of foodborne bacteria. | the inactivation of eight different bacteria comprising escherichia coli lmm1010 and mg1655, respectively a pressure-resistant strain and the corresponding wild-type, salmonella typhimurium, pseudomonas fluorescens, staphylococcus aureus, enterococcus faecalis, listeria innocua and lactobacillus plantarum, by high hydrostatic pressure in skim milk supplemented with the lactoperoxidase-hydrogen peroxide-thiocyanate (lp) system at naturally occurring concentration was studied. in the absence of pr ... | 2003 | 12485747 |
| kinetic model for the inactivation of lactobacillus plantarum by pulsed electric fields. | the kinetics of lactobacillus plantarum inactivation by pulsed electric fields (pef) was studied in two different growth stages (exponential and stationary), but in the same reference medium (0.6% peptone water). electric field intensity and treatment time varied from 20 to 28 kv/cm and 30 to 240 micros, respectively. the experimental data showed that cells in the exponential growth stage were more sensitive to pef treatment than those in the stationary stage. the inactivation data were adjusted ... | 2003 | 12485748 |
| isolation, characterisation and identification of lactic acid bacteria from bushera: a ugandan traditional fermented beverage. | one hundred and thirteen strains of lactic acid bacteria (lab) were selected from 351 isolates from 15 samples of traditionally fermented household bushera from uganda and also from laboratory-prepared bushera. isolates were phenotypically characterised by their ability to ferment 49 carbohydrates using api 50 chl kits and additional biochemical tests. coliforms, yeasts and lab were enumerated in bushera. the ph, volatile organic compounds and organic acids were also determined. the lab counts i ... | 2003 | 12494920 |
| bacterial contamination of cucumber fruit through adhesion. | in this study, the adhesion of bacteria to fresh cucumber surfaces in aqueous suspension was shown to be dependent on time of incubation, inoculum species and concentration, and temperature. the adhesion of bacteria to the fruit in wash water was less extensive at lower temperatures and shorter exposure times. various species of bacteria were adsorbed to cucumber surfaces in the following relative order: salmonella typhimurium > staphylococcus aureus > lactobacillus plantarum > listeria monocyto ... | 2002 | 12495005 |
| esterolytic and lipolytic activities of lactic acid bacteria isolated from ewe's milk and cheese. | in the present work, we report on the esterase and lipase activities of lactic acid bacteria representing the genera lactococcus, leuconostoc, lactobacillus, and enterococcus isolated from ewe's milk and cheeses. esterase activity was studied using alpha- and beta-naphthyl derivatives of 2 to 12 carbon atoms and postelectrophoretic detection. the lactic acid bacteria evaluated had intracellular esterase activities, which preferentially degraded the alpha- and beta-naphthyl derivatives of 2 to 6 ... | 2002 | 12495024 |
| arginine catabolism by sourdough lactic acid bacteria: purification and characterization of the arginine deiminase pathway enzymes from lactobacillus sanfranciscensis cb1. | the cytoplasmic extracts of 70 strains of the most frequently isolated sourdough lactic acid bacteria were screened initially for arginine deiminase (adi), ornithine transcarbamoylase (otc), and carbamate kinase (ck) activities, which comprise the adi (or arginine dihydrolase) pathway. only obligately heterofermentative strains such as lactobacillus sanfranciscensis cb1; lactobacillus brevis am1, am8, and 10a; lactobacillus hilgardii 51b; and lactobacillus fructivorans dd3 and da106 showed all t ... | 2002 | 12450844 |
| purification and genetic characterization of plantaricin nc8, a novel coculture-inducible two-peptide bacteriocin from lactobacillus plantarum nc8. | a new, coculture-inducible two-peptide bacteriocin named plantaricin nc8 (plnc8) was isolated from lactobacillus plantarum nc8 cultures which had been induced with lactococcus lactis mg1363 or pediococcus pentosaceus fbb63. this bacteriocin consists of two distinct peptides, named alpha and beta, which were separated by c(2)-c(18) reverse-phase chromatography and whose complementary action is necessary for full plantaricin nc8 activity. n-terminal sequencing of both purified peptides showed 28 a ... | 2003 | 12514019 |
| phylogenetic diversity of lactic acid bacteria associated with paddy rice silage as determined by 16s ribosomal dna analysis. | a total of 161 low-g+c-content gram-positive bacteria isolated from whole-crop paddy rice silage were classified and subjected to phenotypic and genetic analyses. based on morphological and biochemical characters, these presumptive lactic acid bacterium (lab) isolates were divided into 10 groups that included members of the genera enterococcus, lactobacillus, lactococcus, leuconostoc, pediococcus, and weissella: analysis of the 16s ribosomal dna (rdna) was used to confirm the presence of the pre ... | 2003 | 12514026 |
| antifungal activity of phenyllactic acid against molds isolated from bakery products. | phenyllactic acid (pla) has recently been found in cultures of lactobacillus plantarum that show antifungal activity in sourdough breads. the fungicidal activity of pla and growth inhibition by pla were evaluated by using a microdilution test and 23 fungal strains belonging to 14 species of aspergillus, penicillium, and fusarium that were isolated from bakery products, flours, or cereals. less than 7.5 mg of pla ml(-1) was required to obtain 90% growth inhibition for all strains, while fungicida ... | 2003 | 12514051 |
| effect of lactobacillus plantarum 299v on colonic fermentation and symptoms of irritable bowel syndrome. | a number of recent clinical trials have promoted the use of probiotic bacteria as a treatment for irritable bowel syndrome (ibs). the recent demonstration of abnormal colonic fermentation in some patients with this condition provides an opportunity for the objective assessment of the therapeutic value of these bacteria. this study was designed to investigate the effects of lactobacillus plantarum 299v on colonic fermentation. we conducted a double-blind, placebo-controlled, cross-over, four-week ... | 2002 | 12452404 |
| [inhibition of lactobacillus species on the germination of aspergillus flavus spore]. | actively growing lactobacillus plantarum cgmcc 1.1856 cells totally inhibited the germination of mold spores. cell-free supernatant broth from the fermentation of lactobacillus plantarum could not destroy the viability of mold spore. while the ph of the culture broth and supernatant were about 4.0, and the acidification of non-fermented broth to ph 4.0 with lactic acid could not cause a similar inhibition on spore germination. this experiment discounted the possibility that the inhibitory effect ... | 2002 | 12561574 |
| complete genome sequence of lactobacillus plantarum wcfs1. | the 3,308,274-bp sequence of the chromosome of lactobacillus plantarum strain wcfs1, a single colony isolate of strain ncimb8826 that was originally isolated from human saliva, has been determined, and contains 3,052 predicted protein-encoding genes. putative biological functions could be assigned to 2,120 (70%) of the predicted proteins. consistent with the classification of l. plantarum as a facultative heterofermentative lactic acid bacterium, the genome encodes all enzymes required for the g ... | 2003 | 12566566 |
| differential roles of the two-component peptides of lactocin 705 in antimicrobial activity. | lactobacillus casei crl705 produces a class iib bacteriocin, lactocin 705, which relies on the complementary action of two components, lac705alpha and lac705beta. these peptides exert a bactericidal effect on the indicator strain lactobacillus plantarum crl691, with an optimal lac705alpha/lac705beta peptide ratio of 1 to 4. electron microscopy studies showed that treated crl691 cells have their cell wall severely damaged, with mesosome-like membranous formations protruding into their cytoplasm. ... | 2003 | 12567240 |
| evaluation of the effect of malt, wheat and barley extracts on the viability of potentially probiotic lactic acid bacteria under acidic conditions. | in this work, the effect of cereal extracts, used as delivery vehicles for potentially probiotic lactic acid bacteria (lab), on the acid tolerance of the cells was evaluated under conditions that simulate the gastric tract. more specifically, the effect of malt, barley and wheat extracts on the viability of lactobacillus plantarum, lactobacillus acidophilus and lactobacillus reuteri during exposure for 4 h in a phosphate buffer acidified at ph 2.5 was investigated. in the absence of cereal extra ... | 2003 | 12568753 |
| evolution of microbial populations during traditional feta cheese manufacture and ripening. | in three different dairies (a, b and c) located in peloponess region (southern greece), traditional feta cheese trials took place february to march using mixtures of sheep's and goat's milk. only small variations in the evolution of microbial groups were observed during the whole ripening period. the main groups, such as thermophilic cocci, mesophilic lactococci, thermophilic lactobacilli, nonstarter lactic acid bacteria (nslab), presumptive leuconostoc, enterococci and micrococci, reached their ... | 2003 | 12568755 |
| molecular characterization of tet(m) genes in lactobacillus isolates from different types of fermented dry sausage. | the likelihood that products prepared from raw meat and milk may act as vehicles for antibiotic-resistant bacteria is currently of great concern in food safety issues. in this study, a collection of 94 tetracycline-resistant (tc(r)) lactic acid bacteria recovered from nine different fermented dry sausage types were subjected to a polyphasic molecular study with the aim of characterizing the host organisms and the tet genes, conferring tetracycline resistance, that they carry. with the (gtg)(5)-p ... | 2003 | 12571056 |