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efficient secretion of the model antigen m6-gp41e in lactobacillus plantarum ncimb 8826.four lactobacillus strains (lb. plantarum ncimb 8826, lb. paracasei lbtgs1.4, lb. casei atcc 393 and lb. fermentum kld) were tested for their ability to produce and secrete heterologous proteins. these strains were first screened with an alpha-amylase reporter under the control of a set of expression or expression/secretion signals from various lactic acid bacteria. with most of the constructions tested, the level of extracellular production was highest in lb. plantarum ncimb 8826, and lowest in ...19979274026
effect of added carbohydrates on membrane phase behavior and survival of dried lactobacillus plantarum.the relation between the protective effect of externally added carbohydrates on lactobacillus plantarum cells during air-drying and the phase behavior of cell membranes was studied. the residual activity after drying could be improved from 44% in the control to 79 and 66% after the addition of sorbitol and maltose, respectively, whereas trehalose addition resulted in a residual activity of 30%. membrane phase transition temperatures (tm) were determined in intact hydrated and dry cells, using fo ...19979302766
functional and structural features of the holin hol protein of the lactobacillus plantarum phage phi gle: analysis in escherichia coli system.lactobacillus plantarum phage phi gle has two consecutive cell lysis genes hol-lys (oki et al., 1996b). in the present study, functional and structural properties of the hol protein (hol) were characterized in escherichia coli. electron microscopic examinations showed that hol under plac in e. coli xl1-blue injured the inner membrane to yield empty ghost cells with the bulk of the cell wall undisturbed. northern blot analysis indicated that hol-lys genes under plac were co-transcribed, although ...19979332359
identification and characterization of homofermentative mesophilic lactobacillus strains isolated from artisan starter-free cheeses.fifty-six strains of mesophilic lactobacilli from hand-made cheeses made without starters have been isolated, identified and characterized. of these, 21 strains were classified as lactobacillus plantarum, 18 as lact. casei subsp. pseudoplantarum, 10 as lact. curvatus, five as lact. casei subsp. casei, and two remained unidentified. the numerical classification of these strains, based on 80 different physiological and morphological characteristics, correlated well with the phenotypic classificati ...19979351268
flow cytometric analysis of lactobacillus plantarum to monitor lag times, cell division and injury.flow cytometry in combination with fluorescent molecular markers 5- (and 6-) carboxyfluorescein succinimidylester (cfse) and propidium iodide (pi) have been applied to determine lag times, numbers of cell divisions and injury after mild heat (50 degrees c, 5 min) and nisin treatments (0.1 and 1.0 microgram ml-1) of lactobacillus plantarum. initial labelling with covalently bound dye cfse (20 and 100 micrograms ml-1) allowed determination of lag times and cell proliferation for up to eight genera ...19979351280
effects of lactobacilli on yeast-catalyzed ethanol fermentations.normal-gravity (22 to 24 degrees plato) wheat mashes were inoculated with five industrially important strains of lactobacilli at approximately 10(5), approximately 10(6), approximately 10(7), approximately 10(8), and approximately 10(9) cfu/ml in order to study the effects of the lactobacilli on yeast growth and ethanol productivity. lactobacillus plantarum, lactobacillus paracasei, lactobacillus #3, lactobacillus rhamnosus, and lactobacillus fermentum were used. controls with yeast cells but no ...19979361399
molecular characterization of the alpha-amylase genes of lactobacillus plantarum a6 and lactobacillus amylovorus reveals an unusual 3' end structure with direct tandem repeats and suggests a common evolutionary origin.the alpha-amylase gene (amya) of lactobacillus plantarum a6 was isolated from the genome by polymerase chain reaction with degenerated oligonucleotides, synthesized according to the tryptic peptide amino acid sequences of the purified enzyme. nucleic acid sequence analysis revealed one open reading frame of 2739 bp encoding a 913 amino acid protein. the amylase appears to be divided into two equal parts. the n-terminal part has the typical characteristics of the well-known alpha-amylase family ( ...19979370276
aggregation-promoting factor in human vaginal lactobacillus strains.a total of 60 lactobacillus sp. strains were examined for expression of auto-aggregation and cell surface hydrophobicity. isolates were obtained from the vagina of healthy women (n = 20). the results obtained showed that the occurrence of cell surface hydrophobicity correlated with auto-aggregative activity in 12 homofermentative lactobacillus sp. strains. the aggregation mechanism was mediated by the presence of an aggregation promoting factor (apf) in one lactobacillus sp. strain, hv 142. apf ...19979395055
purification and dna-binding properties of the integrase protein int encoded by lactobacillus plantarum phage.the lactobacillus plantarum temperate phage phi g1e (42,259 bp) encodes an integrase gene int linked to a phage attachment site attp (kakikawa et al., 1997). to investigate phi g1e recombination, the integrase protein int was overproduced in escherichia coli under the t7 promoter, and purified. the int protein had an apparent molecular mass of 42.0 kda, corresponding well with that (45.5 kda) predicted from the dna sequence. amino-acid sequencing revealed that the n-terminal 20 amino-acids of th ...19979434187
isolation and characterization of a tributyrin esterase from lactobacillus plantarum 2739.an intracellular tributyrin esterase from lactobacillus plantarum 2739 was purified to homogeneity by chromatography on deae cellulose, sephacryl 200, carboxymethylcellulose, and mono q. the enzyme e2 was separated on deae cellulose from a second esterase, e1, and a minor esterase. additional minor esterases were separated from e2 during chromatography on sephacryl. e2 was a monomer with a relative molecular mass of approximately 85 kda. the enzyme was most active at ph 7 and 35 degrees c and re ...19979436090
characterization of a bacteriophage for carnobacterium divergens ncfb 2763 by host specificity and electron microscopy.carnobacterium divergens ncfb 2763 was used to isolate bacteriophage cd1 from minced beef using an enrichment isolation technique. host specificity testing showed that it exhibited lytic activity only against a limited number of c. divergens strains. no activity was observed against any of the c. gallinarum, c. mobile, c. piscicola, enterococcus durans, lactobacillus plantarum and lact. brevis strains tested. anaerobic incubation had no effect on the host specificity pattern. bacteriophage cd1 b ...19979449854
electrotransformation of lactobacillus plantarum using linearized plasmid dna.evidence is presented that linearized plasmid dna is capable of electrotransforming lactobacillus plantarum at a frequency 500-fold lower than with the covalently closed circular molecule. when the linearized plasmid was religated prior to transformation the transformation efficiency was < 10-fold higher, suggesting that open circular plasmid was only slightly more efficient in the transformation of lact. plantarum than linear dna. this observation has implications for direct cloning into this s ...19979449855
gene cloning, transcriptional analysis, purification, and characterization of phenolic acid decarboxylase from bacillus subtilis.bacillus subtilis displays a substrate-inducible decarboxylating activity with the following three phenolic acids: ferulic, p-coumaric, and caffeic acids. based on dna sequence homologies between the bacillus pumilus ferulate decarboxylase gene (fdc) (a. zago, g. degrassi, and c. v. bruschi, appl. environ. microbiol. 61:4484-4486, 1995) and the lactobacillus plantarum p-coumarate decarboxylase gene (pdc) (j.-f. cavin, l. barthelmebs, and c. diviès, appl. environ. microbiol. 63:1939-1944, 1997), ...19989546183
cocoa fermentations conducted with a defined microbial cocktail inoculum.cocoa fermentations were performed in wooden boxes under the following four experimental regimens: beans naturally fermented with wild microflora; aseptically prepared beans with no inoculum; and beans inoculated with a defined cocktail containing microorganisms at a suitable concentration either at zero time or by using phased additions at appropriate times. the cocktail used consisted of a yeast, saccharomyces cerevisiae var. chevalieri, two lactic acid bacterial species, lactobacillus lactis ...19989546184
amino acid decarboxylase capability of microorganisms isolated in spanish fermented meat products.enterobacteria, lactic acid bacteria (lab) and gram-positive cocci were isolated from spanish meat products. the most frequent species in the meat products studied were identified as lactobacillus sake, lactobacillus plantarum and lactobacillus curvatus from de man-rogosa-sharpe agar; staphylococcus xylosus, staphylococcus saprophyticus and micrococcus varians from mannitol salt phenol-red agar; and hafnia alvei, escherichia coli, pseudomonas fluorescens, enterobacter amnigenes and enterobacter ...19989553801
rapid species identification within two groups of closely related lactobacilli using pcr primers that target the 16s/23s rrna spacer region.a rapid and reliable pcr-based method for distinguishing closely related species within two groups of lactobacilli is described. primers complementary to species-specific sequences in the 16s/23s rdna spacer regions were designed after sequencing and sequence comparison of the spacer regions of 32 strains. the strains belong to two groups of closely related lactobacillus species; one composed of lactobacillus curvatus, lactobacillus graminis and lactobacillus sake, the other of lactobacillus par ...19989561736
cytotoxicity of heat-treated korean mistletoe.the cytotoxic effects of preparations of korean mistletoe (viscum album l. var. coloratum ohwi) on non-tumorigenic a31 cells and tumorigenic msv cells were investigated. while the aqueous extract from korean mistletoe (<8 microg/ml) showed strong cytotoxicity on both cell lines, the heat-treated extract was much less cytotoxic with td50 values of above 300 microg/ml. the heat-treated extract showed a growth-enhancing effect on non-tumorigenic cells and a cytotoxic effect on tumorigenic cells. th ...19989563647
plantaricin d, a bacteriocin produced by lactobacillus plantarum bfe 905 ready-to-eat salad.lactobacillus plantarum bfe 905 isolated from 'waldorf' salad produced a bacteriocin termed plantaricin d which was active against lact. sake and listeria monocytogenes strains. plantaricin d was heat stable, retaining activity after heating at 121 degrees c. the bacteriocin was inactivated by alpha-chymotrypsin, trypsin, pepsin and proteinase k, but not by papain and other non-proteolytic enzymes tested. plantaricin d was stable at ph values ranging from 2.0 to 10.0. the bacteriocin inhibited g ...19989569716
molecular analysis of the locus responsible for production of plantaricin s, a two-peptide bacteriocin produced by lactobacillus plantarum lpco10.a 4.5-kb region of chromosomal dna carrying the locus responsible for the production of plantaricin s, a two-peptide bacteriocin produced by lactobacillus plantarum lpco10 (r. jiménez-díaz, j. l. ruiz-barba, d. p. cathcart, h. holo, i. f. nes, k. h. sletten, and p. j. warner, appl. environ. microbiol. 61:4459-4463, 1995), has been cloned, and the nucleotide sequence has been elucidated. two genes, designated plsa and plsb and encoding peptides alpha and beta, respectively, of plantaricin s, plus ...19989572965
lactic acid fermentation and storage of blanched garlic.the controlled fermentation of peeled, blanched garlic, using a starter culture of lactobacillus plantarum, was studied and compared with that of unblanched garlic. blanching was carried out in hot water (90 degrees c) for 15 min. the starter grew abundantly in the case of blanched garlic, producing mainly lactic acid and reaching a ph of 3.8 after 7 days, but its growth was inhibited in unblanched garlic. ethanol and fructose, coming from enzymatic activities of the garlic, and a green pigment ...19989580446
activation of thiamin diphosphate and fad in the phosphatedependent pyruvate oxidase from lactobacillus plantarum.the phosphate- and oxygen-dependent pyruvate oxidase from lactobacillus plantarum is a homotetrameric enzyme that binds 1 fad and 1 thiamine diphosphate per subunit. a kinetic analysis of the partial reactions in the overall oxidative conversion of pyruvate to acetyl phosphate and co2 shows an indirect activation of the thiamine diphosphate by fad that is mediated by the protein moiety. the rate constant of the initial step, the deprotonation of c2-h of thiamine diphosphate, increases 10-fold in ...19989582325
plantaricin lp84, a broad spectrum heat-stable bacteriocin of lactobacillus plantarum ncim 2084 produced in a simple glucose broth medium.lactobacillus plantarum ncim 2084 produced an antibacterial substance when grown at 40 degrees c for 36 h in a laboratory medium. the antibacterial substance was active against a wide range of bacteria comprising gram positive and negative foodborne pathogenic and spoilage bacteria. however, related mesophilic lactobacilli were not inhibited, except for l. amylovorus dsm 20531. the antimicrobial activity was observed between late log and stationary growth phases. the antibacterial substance was ...19989600606
isolation and characterization of pediocin ach chimeric protein mutants with altered bactericidal activity.a collection of pediocin ach amino acid substitution mutants was generated by pcr random mutagenesis of dna encoding the bacteriocin. mutants were isolated by cloning mutagenized dna into an escherichia coli male plasmid that directs the secretion of maltose binding protein-pediocin ach chimeric proteins and by screening transformant colonies for bactericidal activity against lactobacillus plantarum ncdo955 (k. w. miller, r. schamber, y. chen, and b. ray, 1998. appl. environ. microbiol. 64:14-20 ...19989603806
antagonistic activity of lactobacillus plantarum c11: two new two-peptide bacteriocins, plantaricins ef and jk, and the induction factor plantaricin a.six bacteriocinlike peptides (plantaricin a [plna], plne, plnf, plnj, plnk, and plnn) produced by lactobacillus plantarum c11 were detected by amino acid sequencing and mass spectrometry. since purification to homogeneity was problematic, all six peptides were obtained by solid-phase peptide synthesis and were tested for bacteriocin activity. it was found that l. plantarum c11 produces two two-peptide bacteriocins (plnef and plnjk); a strain-specific antagonistic activity was detected at nanomol ...19989603847
decrease in fibrinogen and ldl-cholesterol levels upon supplementation of diet with lactobacillus plantarum in subjects with moderately elevated cholesterol. 19989622287
combined influence of growth and drying conditions on the activity of dried lactobacillus plantarumthe production of active dried starter cultures can be influenced at several levels in the production process. in this paper the following process factors are discussed: osmotic stress during growth and cell density prior to drying. contradicting results are reported in the literature on the influence of osmotic stress during growth on the residual activity after drying. the combined approach in which two process factors were studied at a time resulted in an explanation for the discrepancy in ea ...19989622539
qsars for the effect of benzaldehydes on foodborne bacteria and the role of sulfhydryl groups as targets of their antibacterial activity.quantitative structure activity relationships (qsars) were obtained describing the activity of a series of benzaldehydes against three different foodborne bacteria, listeria monocytogenes f6861, serotype 4b, salmonella enteritidis, phage type 4, p167807 and lactobacillus plantarum int.l11. mic values at ph 6.2 and 35 degrees c were obtained for 11 phenolic benzaldehydes to produce multiple linear regression and artificial neural network models. for each organism, the models contained a steric pa ...19989633634
expression of the bglh gene of lactobacillus plantarum is controlled by carbon catabolite repression.a newly identified bglh gene coding for a phospho-beta-glucosidase of lactobacillus plantarum was isolated and expressed in escherichia coli. the sequence analysis of the cloned dna fragment showed an open reading frame encoding a 480-amino-acid protein with a calculated molecular mass of 53 kda. the bglh gene was shown to be expressed on a monocistronic transcriptional unit. its transcription was repressed 10-fold in l. plantarum cells grown on glucose compared to the beta-glucoside salicin as ...19989642194
sensorial and microbial effects of gaseous ozone on fresh scad (trachurus trachurus).the bactericidal activity of gaseous ozone was investigated using a commercial ozone generator. five species of fish bacteria, pseudomonas putida, shewanella putrefaciens, brochothrix thermosphacta, enterobacter sp. and lactobacillus plantarum, were inoculated on agar surfaces and exposed to different ozonation times in a gas chamber. results showed ozone in relatively low concentrations (< 0.27 x 10(-3) g l-1) was an effective bactericide of vegetative cells of the five fish bacteria. the age o ...19989674134
heat-killed lactobacillus plantarum l-137 suppresses naturally fed antigen-specific ige production by stimulation of il-12 production in mice.food allergy is caused by production of ige against dietary antigen induced by t(h2) response. il-12 inhibits t(h2) responses and strongly suppresses ige production. we have recently established a murine model for ige production with a predominant t(h2) response induced by feeding antigen.19989679848
functional expression in lactobacillus plantarum of xylp encoding the isoprimeverose transporter of lactobacillus pentosus.the xylp gene of lactobacillus pentosus, the first gene of the xylpqr operon, was recently found to be involved in isoprimeverose metabolism. by expression of xylp on a multicopy plasmid in lactobacillus plantarum 80, a strain which lacks active isoprimeverose and d-xylose transport activities, it was shown that xylp encodes a transporter. functional expression of the xylp transporter was shown by uptake of isoprimeverose in l. plantarum 80 cells, and this transport was driven by the proton moti ...19989683504
immunonutrition: role of biosurfactants, fiber, and probiotic bacteria.phospholipids constitute an important part of cellular membranes, and membrane fluidity and permeability are dependent on the fatty acid composition of the phospholipid. the composition, which changes with aging and disease is, to a large degree, influenced by nutrient supply. phospholipids have been effective in protecting cellular membranes such as those of the gastrointestinal tract to an extent not much different from that observed with external supply of established mucosa-protective drugs ...19989684261
conversion of phenylalanine to benzaldehyde initiated by an aminotransferase in lactobacillus plantarumthe production of benzaldehyde from phenylalanine has been studied in various microorganisms, and several metabolic pathways have been proposed in the literature for the formation of this aromatic flavor compound. in this study, we describe benzaldehyde formation from phenylalanine by using a cell extract of lactobacillus plantarum. phenylalanine was initially converted to phenylpyruvic acid by an aminotransferase in the cell extract, and the keto acid was further transformed to benzaldehyde. ho ...19989687465
treatment strategies for small bowel bacterial overgrowth in short bowel syndrome.small bowel bacterial overgrowth is a common complication of short bowel syndrome, and although it is often controlled with antimicrobial therapy, alternative strategies may occasionally be needed.19989702645
survival of lactobacillus plantarum dsm 9843 (299v), and effect on the short-chain fatty acid content of faeces after ingestion of a rose-hip drink with fermented oats.in a controlled and randomised double-blind study, 26 healthy adult volunteers consumed, for 21 d, 400 ml of a rose-hip drink containing oats (0.7 g/100ml) fermented with lactobacillus plantarum dsm 9843 (rhl; containing 5 x 10(7) cfu ml(-1)), and 22 volunteers in a second group the same amount of a pure rose-hip drink (rh). significant increases in the total faecal concentration of carboxylic acids (p < 0.05 after 1 week and p < 0.01 after 3 weeks of intake), acetic acid (p < 0.01 after 3 weeks ...19989706795
inhibition of listeria monocytogenes in a smear-surface soft cheese by lactobacillus plantarum whe 92, a pediocin ach producer.the anti-listeria monocytogenes activity of lactobacillus plantarum whe 92, a pediocin ach producer, was investigated in munster cheese, a smear-surface soft cheese. the appearance of l. monocytogenes in the cheese, which naturally occurs solely in the crust and never before 1 week of ripening, could be prevented by spraying a cell suspension of l. plantarum whe 92 (ca. 10(5) cfu/ml) on the cheese surface at the beginning of the ripening period. l. monocytogenes was sometimes detected at low lev ...19989708279
promoter/repressor system of lactobacillus plantarum phage og1e: characterization of the promoters pr49-pr-pl and overproduction of the cro-like protein cng in escherichia coli.the lactobacillus plantarum phage og1e (42<hsp sp = "0. 25">259<hsp sp = "0.25">bp) has two repressor-like genes cng and cpg oriented oppositely, accompanied by three potential promoters pr, pl and pr49, and seven operator-like sequences (gatac-boxes) (kodaira et al., 1997). in this study, the og1e putative promoters were introduced into the escherichia coli promoter-detecting plasmid pkk232-8. in e. coli ck111, pr (pkpr1), pl (pkpl1) and pr49 (pkpr49) exhibited distinct cat activities. when pkp ...19989714836
isolation, purification and partial characterization of plantaricin 423, a bacteriocin produced by lactobacillus plantarum.lactobacillus plantarum 423, isolated from sorghum beer, produces a bacteriocin (plantaricin 423) which is inhibitory to several food spoilage bacteria and food-borne pathogens, including bacillus cereus, clostridium sporogenes, enterococcus faecalis, listeria spp. and staphylococcus spp. plantaricin 423 is resistant to treatment at 80 degrees c, but loses 50% of its activity after 60 min at 100 degrees c and 75% of its activity after autoclaving (121 degrees c, 15 min). plantaricin 423 remains ...19989717299
physiological response of lactobacillus plantarum to salt and nonelectrolyte stress.in this report, we compared the effects on the growth of lactobacillus plantarum of raising the medium molarity by high concentrations of kcl or nacl and iso-osmotic concentrations of nonionic compounds. analysis of cellular extracts for organic constituents by nuclear magnetic resonance spectroscopy showed that salt-stressed cells do not contain detectable amounts of organic osmolytes, whereas sugar-stressed cells contain sugar (and some sugar-derived) compounds. the cytoplasmic concentrations ...19989721316
inhibition of listeria monocytogenes by plantaricin na, an antibacterial substance from lactobacillus plantarum.a lactobacillus plantarum of vegetable origin produced a bacteriocin inhibitor to listeria monocytogenes. the antimicrobial agent was inactivated by proteolytic enzymes, was resistant to heat (100 degrees c for 30 min) and stable over a wide ph range (ph 2-10), and displayed a bactericidal mode of action. growth inhibition of l. monocytogenes depend on bacteriocin concentration. the antilisterial efficiency depended on the strain of l. monocytogenes used but was not influenced by the growth phas ...19989721606
the normal lactobacillus flora of healthy human rectal and oral mucosa.the lactobacillus flora of the rectal and oral mucosa was sampled from 42 healthy volunteers. species identification was carried out by numerically comparing api 50ch fermentation patterns with type strains, using an sj-similarity cut-off level of 79%. for the largest groups, identity was further confirmed by dna-dna hybridizations against the type strain of the species. seventeen lactobacilli clusters were defined, of which most were found both on rectal and oral mucosa. the largest taxa were l ...19989721659
chemostat production of plantaricin c by lactobacillus plantarum ll441.plantaricin c, a bacteriocin synthesized by lactobacillus plantarum ll441, was optimally produced in chemostats kept at ph 5.0, 30 degreesc, 150 rpm, and a dilution rate of 0.05 h-1 when glucose was used as carbon source and a dilution rate of 0.10 to 0.12 h-1 when sucrose or fructose was used instead. production was abolished at high dilution rates, i.e., when the cells grew rapidly in all carbon sources.19989726907
identification of the dna-binding sites for two response regulators involved in control of bacteriocin synthesis in lactobacillus plantarum c11.in lactobacillus plantarum c11, bacteriocin production has previously been shown to be an inducible process, in which a secreted peptide, produced by the host itself, is involved. the inducing factor, designated plantaricin a (plna), is a bacteriocin-like peptide encoded by a gene (plna) located on the same operon as the genes for a two-component regulatory system (plnbcd). this system consists of a histidine kinase (plnb) and two response regulators (plnc,d), and belongs to a recently defined s ...19989747714
synergistic antibacterial action of heat in combination with nisin and magainin ii amide.lactobacillus plantarum has been exposed to mild heat at temperatures between 48 and 56 degrees c in combination with low concentrations of the lantobiotic nisin in different sequential set-ups. exposure to heat and nisin caused synergistic reductions of lact. plantarum viability. efficient antimicrobial action was dependent on the growth state of the culture as well as on levels and sequences of treatment applications. listeria monocytogenes and escherichia coli were treated at 55 degrees c in ...19989750279
bacteriocin production and competitiveness of lactobacillus plantarum lpco10 in olive juice broth, a culture medium obtained from olives.a culture medium, named olive juice broth, which resembles the natural environment of lactobacillus plantarum in the traditional spanish-style green olive fermentation was obtained from green olives. in this medium, the bacteriocin-producing l. plantarum lpco10 strain was able to produce bacteriocin throughout the incubation time (15 days). bacteriocin purification from olive juice broth was achieved by a protocol including ammonium sulphate precipitation of cell-free, l. plantarum lpco10 cultur ...19989761347
effects of tamoxifen, melatonin, coenzyme q10, and l-carnitine supplementation on bacterial growth in the presence of mycotoxins.the involvement of toxic oxygen intermediates in the bacteriostatic effects of mycotoxins (t-2 toxin, deoxynivalenol, ochratoxin a, aflatoxin b1, and fumonisin b1) was investigated by producing bacterial growth curves using turbidimetry assays in the presence and absence of oxygen radical-scavenging substances. the strains used in this study included escherichia coli (ft 101), streptococcus agalactiae (ft 311, ft 313, ft 315), staphylococcus aureus (ft 192), yersinia enterocolitica (ft 430), sal ...19989774492
mechanism of osmotic activation of the quaternary ammonium compound transporter (qact) of lactobacillus plantarum.the accumulation of quaternary ammonium compounds in lactobacillus plantarum is mediated via a single transport system with a high affinity for glycine betaine (apparent km of 18 microm) and carnitine and a low affinity for proline (apparent km of 950 microm) and other analogues. mutants defective in the uptake of glycine betaine were generated by uv irradiation and selected on the basis of resistance to dehydroproline (dhp), a toxic proline analogue. three independent dhp-resistant mutants show ...19989791101
[use of exogenous ribonuclease for improvement of lactobacillus biological properties].the influence of exogenous rnase on the dynamics of the acid formation by the industrial strain 8r-a3 of lactobacillus plantarum, its antibiotic sensitivity and antagonistic activity was studied. in the presence of the rnaase growth stimulating dose both a decrease of the culture lag-phase and a more intensive accumulation of lactic acid in the incubation medium resulting in an increase of the lactobacillus antagonistic activity were observed. it was shown that rnaase increased the lactobacillus ...19989791680
rice hull ash and silicic acid as adsorbents for concentration of bacteriocinsa model procedure has been developed for the rapid extraction of five bacteriocins (nisin, pediocin rs2, leucocin bc2, lactocin gi3, and enterocin cs1) from concentrated freeze-dried crude culture supernatants by adsorption onto acid or alkaline rice hull ash (rha) or silicic acid (sa). bacteriocins were adsorbed onto rha or sa by a ph-dependent method and desorbed by decreasing the ph to 2.5 or 3.0 and heating at 90 degreesc for 5 min. the maximum adsorption and optimal ph range for different b ...19989797298
incorporation of food-grade antimicrobial compounds into biodegradable packaging films.lysozyme and nisin are both antimicrobial proteins effective against gram-positive bacteria. the use of these antimicrobials in combination with chelating agents displays increased effectiveness against gram-negative bacteria. packaging films with lysozyme or nisin incorporated into the film structure were tested separately for inhibition against lactobacillus plantarum. both lysozyme and nisin were used in combination with edta in films and were evaluated for inhibition against escherichia coli ...19989798150
plantaricin a is an amphiphilic alpha-helical bacteriocin-like pheromone which exerts antimicrobial and pheromone activities through different mechanisms.production of bacteriocins by lactic acid bacteria is in some cases regulated by a quorum sensing mechanism that involves a secreted bacteriocin-like peptide pheromone. in the case of lactobacillus plantarum c11, this pheromone, the 26-mer plantaricin a (plna), has the interesting property of having both bacteriocin and pheromone activities. to gain insight into how plna functions as a pheromone and as a bacteriocin, the l- and d-enantiomers of an n-terminally truncated form of plna were synthes ...19989819195
protein modification by fermentation: effect of fermentation on the potential allergenicity of pea.the effect of fermentation on components of potential significance for the allergenicity of pea was analyzed. pea flour was fermented with three lactic acid bacteria, pediococcus pentosaceus, lactococcus raffinolactis, and lactobacillus plantarum, and two fungi, rhizopus microsporus, var. oligosporus and geotrichum candidum. residual antigenicity against antipea antibodies was reduced to 10% by the three lactic acid bacteria and r. microsporus. reactions to anti-pea profilin and anti-bet v 1 wer ...19989826013
the effect of dietary fatty acids on lactic acid bacteria associated with the epithelial mucosa and from faecalia of arctic charr, salvelinus alpinus (l.).arctic charr, salvelinus alpinus (l.), held in fresh water, were fed four experimental diets containing different polyunsaturated fatty acids (pufa). in addition, one group fed a diet containing only coconut oil as sole lipid source served as control. the population of aerobic heterotrophic bacteria associated with the epithelial mucosa and the faecalia was estimated using the dilution plate technique. generally, the population level of adherent bacteria increased along the digestive tract (stom ...19989830121
effect of leavening microflora on pizza dough properties.fourteen different starter cultures containing one strain of saccharomyces cerevisiae with and without individual or combinations of lactic acid bacteria (lactobacillus plantarum, lact. sanfrancisco, enterococcus faecium, leuconostoc mesenteroides) were employed to investigate the role of leavening microflora on the properties of pizza doughs. microbiological, chemical and physical characteristics of doughs prepared with the same flour and under the same processing conditions were determined. le ...19989830125
molecular cloning and functional expression in lactobacillus plantarum 80 of xylt, encoding the d-xylose-h+ symporter of lactobacillus brevis.a 3-kb region, located downstream of the lactobacillus brevis xyla gene (encoding d-xylose isomerase), was cloned in escherichia coli tg1. the sequence revealed two open reading frames which could code for the d-xylulose kinase gene (xylb) and another gene (xylt) encoding a protein of 457 amino acids with significant similarity to the d-xylose-h+ symporters of e. coli, xyle (57%), and bacillus megaterium, xylt (58%), to the d-xylose-na+ symporter of tetragenococcus halophila, xyle (57%), and to ...19989835554
discrimination among pediocin ach/pa-1 producer strains by comparison of pedb and pedd amplified genes and by multiplex pcr assay.pediococcus acidilactici, pediococcus parvulus and lactobacillus plantarum pediocin ach/pa-1 produces strains were studied with the aim to investigate their common genetic pediocin determinant using peda, pedb, pedc and pedd gene-targeted pcr assay. single strand conformation polymorphism and restriction analysis of peda and pedc amplified fragments from the three different species did not show any differences in sequence while these analysis carried out on pedb and pedd amplified fragments high ...19989841130
inhibition of nitric oxide production and the effects of arginine and lactobacillus administration in an acute liver injury model.to study the effect of inhibiting nitric oxide production and the effects of arginine and lactobacilli administration in an acute liver injury (li) model.19989860473
synergist effect of sucrose fatty acid esters on nisin inhibition of gram-positive bacteria.nisin in combination with the sucrose fatty acid esters, sucrose palmitate (p-1570 and p-1670) or sucrose stearate (s-1570 and s-1670) was tested against a range of gram-negative and gram-positive bacteria. initial liquid culture investigation showed that the sugar ester p-1670 resulted in a synergist enhancement of the bacteriostatic activity of nisin against gram-positive bacteria and not gram-negative bacteria. some enhancement of the bactericidal activity of nisin against listeria monocytoge ...19989871322
wax d of mycobacterium tuberculosis induced osteomyelitis accompanied by reactive bone formation in buffalo rats.a suspension of heat-killed mycobacterium tuberculosis in liquid paraffin has been reported to induce foot swelling accompanied by new bone formation in buffalo (buf) rats, which are low responders to the induction of adjuvant arthritis. in the present study, we found that wax d, a mycobacterial cell wall peptidoglycan fragment-arabinogalactan-mycolic acid complex, was an effective component of this bacterium for the induction of osteomyelitis accompanied by reactive bone formation in buf rats. ...19989879920
escherichia coli and lactobacillus plantarum responses to osmotic stress.escherichia coli and lactobacillus plantarum were subjected to final water potentials of -5.6 mpa and -11.5 mpa with three solutes: glycerol, sorbitol and nacl. the water potential decrease was realized either rapidly (osmotic shock) or slowly (20 min) and a difference in cell viability between these conditions was only observed when the solute was nacl. the cell mortality during osmotic shocks induced by nacl cannot be explained by a critical volume decrease or by the intensity of the water flo ...19989891930
incorporation of urea nitrogen into fecal protein and plasma protein amino acids in elderly human volunteers after ingestion of lactic acid bacteria.health effects of fermented milks have been associated with the metabolic activity of lactic acid bacteria in the gastrointestinal tract. it has been proposed that an increased excretion of urea nitrogen via microbial protein may decrease the workload on kidneys and liver. therefore, a study was carried out in healthy elderly human subjects to investigate the incorporation of [15n2]urea nitrogen into plasma and fecal proteins and amino acids. over a period of 10 d 13 healthy elderly subjects ing ...19989894687
membrane-mimicking entities induce structuring of the two-peptide bacteriocins plantaricin e/f and plantaricin j/k.lactobacillus plantarum c11 produces plantaricin e/f (plne/f) and plantaricin j/k (plnj/k), two bacteriocins whose activity depends on the complementary action of two peptides (plne and plnf; plnj and plnk). three of the individual pln peptides possess some antimicrobial activity, but the highest bacteriocin activity is obtained by combining complementary peptides in about a one-to-one ratio. circular dichroism was used to study the structure of the pln peptides under various conditions. all fou ...19999922235
oat bran beta-gluco- and xylo-oligosaccharides as fermentative substrates for lactic acid bacteria.the influence of oat bran oligosaccharides on carbohydrate utilization and fermentation end-products was studied with reference to three different lactic acid bacteria (lab: lactobacillus rhamnosus, lactobacillus plantarum and lactococcus lactis). the main results were that all three lab utilized oat beta-gluco-oligosaccharides, while only l. plantarum utilized xylo-oligosaccharides. the main products of lab metabolism were lactic acid, acetic acid, formic acid and ethanol. the results indicated ...19989924948
identification of lactic acid bacteria from chili bo, a malaysian food ingredient.ninety-two strains of lactic acid bacteria (lab) were isolated from a malaysian food ingredient, chili bo, stored for up to 25 days at 28 degreesc with no benzoic acid (product a) or with 7,000 mg of benzoic acid kg-1 (product b). the strains were divided into eight groups by traditional phenotypic tests. a total of 43 strains were selected for comparison of their sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) whole-cell protein patterns with a sds-page database of lab. iso ...19999925588
purification and cloning of a thermostable manganese catalase from a thermophilic bacterium.we have purified a heat-stable catalase from a thermophilic bacterium, thermus species strain ys 8-13. the enzyme was purified 160-fold from crude cellular extracts and possessed a specific activity of 8000 units/mg at 65 degrees c. the purified enzyme displayed the highest activity at ph 7 to 10 and temperatures around 85 degrees c. the catalase was determined to be a manganese catalase, based on results from atomic absorption spectra and inhibition experiments using sodium azide. the enzyme wa ...19999989945
the effect of probiotic strains on the microbiota of the simulator of the human intestinal microbial ecosystem (shime).the aim of the present work was to study five potential probiotic strains (lactobacillus plantarum, two strains of l. paracasei subsp. paracasei, l. rhamnosus and bifidobacterium sp.) comparatively in the simulator of the human intestinal microbial ecosystem (shime) in vitro model, and to evaluate this model as a tool in the screening and selection of probiotic bacteria. the impact of the strains on the composition of microbiota and its metabolic activities (production of lactic acid and short-c ...199910050686
the expression signals of the lactobacillus brevis slpa gene direct efficient heterologous protein production in lactic acid bacteria.a cassette based on the expression signals of the lactobacillus brevis surface (s)-layer protein gene (slpa) was constructed. the low-copy-number vector pkth2095, derived from pgk12, was used as the cloning vector. the efficiency of slpa promoters in intracellular protein production was studied using three reporter genes, beta-glucuronidase (gusa), luciferase (luc) and aminopeptidase n (pepn) in three different lactic acid bacteria hosts: lactococcus lactis, lactobacillus plantarum and lactobaci ...199910077822
influence of environmental factors on lipase production by lactobacillus plantarum.a strain of lactobacillus plantarum, dsmz 12028 (deutsch sammlung von mikroorganismen und zellkulturen), isolated from a portuguese dry fermented sausage, "chouriço", was found to produce true lipase, producing free fatty acids from triolein (olive oil). this enzymatic activity was found in whole cells, but was negligible in comparison to lipolytic activity in culture supernatant. therefore, only extracellular activity was studied. the effect of ph, temperature and glucose concentration on extra ...199910091332
characterization of lactic acid bacteria isolated from a thai low-salt fermented fish product and the role of garlic as substrate for fermentation.lactic acid bacteria (lab) isolated from raw materials (fish, rice, garlic and banana leaves) and processed som-fak (a thai low-salt fermented fish product) were characterized by api 50-ch and other phenotypic criteria. lactococcus lactis subsp. lactis and leuconostoc citreum were specifically associated with fish fillet and minced fish, lactobacillus paracasei subsp. paracasei with boiled rice and weisella confusa with garlic mix and banana leaves. in addition, lactobacillus plantarum, lactobac ...199910100902
immunopotentiating activity of nigerooligosaccharides for the t helper 1-like immune response in mice.the immunopotentiating activity of nigerooligosaccharides (nos), a mixture of nigerose, nigerosyl glucose and nigerosyl maltose, was studied in vitro and in vivo in mice. mitogen-induced proliferation of splenocytes from normal mice was augmented in a dose-dependent manner by nigerose of nos. nos enhanced interleukin 12 (il-12) and interferon-gamma (ifn-gamma) production by normal splenocytes in the presence of the potent il-12 inducer, heat-killed lactobacillus plantarum l-137, in vitro. consis ...199910192919
effect of applying lactic acid bacteria isolated from forage crops on fermentation characteristics and aerobic deterioration of silage.two selected strains, lactobacillus casei fg 1 and lactobacillus plantarum fg 10 that were isolated from forage crops were used as additives at 1.0 x 10(5) cfu/g of fresh matter to alfalfa, italian ryegrass, and sorghum, and their effect on fermentation characteristics and aerobic deterioration of silage was studied. the three silages treated with strains fg 1 or fg 10 were well preserved; had significantly lower ph values, butyric acid, propionic acid, and ammonia n concentrations, gas producti ...199910194670
effect of elevated oxygen and carbon dioxide on the surface growth of vegetable-associated micro-organisms.the impact of a novel type of modified atmosphere (ma), referred to as high o2-ma, on micro-organisms associated with the spoilage of minimally-processed vegetables was studied. pure cultures of pseudomonas fluorescens, enterobacter agglomerans, aureobacterium strain 27, candida guilliermondii, c. sake, salmonella typhimurium, salm. enteritidis, escherichia coli, listeria monocytogenes, leuconostoc mesenteroides var. mesenteroides, lactobacillus plantarum and lactococcus lactis were cultured on ...199910196748
probiotics inhibit enteropathogenic e. coli adherence in vitro by inducing intestinal mucin gene expression.probiotic agents, live microorganisms with beneficial effects for the host, may offer an alternative to conventional antimicrobials in the treatment and prevention of enteric infections. the probiotic agents lactobacillus plantarum 299v and lactobacillus rhamnosus gg quantitatively inhibited the adherence of an attaching and effacing pathogenic escherichia coli to ht-29 intestinal epithelial cells but did not inhibit adherence to nonintestinal hep-2 cells. ht-29 cells were grown under conditions ...199910198338
new types of antimicrobial compounds produced by lactobacillus plantarum.new types of antimicrobial compounds were identified in the culture filtrate of lactobacillus plantarum vtt e-78076. activity was detected in the low molecular mass fraction separated by gel chromatography. this fraction totally inhibited the growth of the gram-negative test organism, pantoea agglomerans (enterobacter agglomerans) vtt e-90396. characteristic compounds from this fraction were identified by gc/ms-analysis and the identification was confirmed using pure commercial reference compoun ...199910200070
immunomodulatory effects of lactobacillus plantarum colonizing the intestine of gnotobiotic rats.we have studied the effect of the probiotic strain lactobacillus plantarum 299v on the immune functions of gnotobiotic rats. one group of germ-free rats was colonized with the type 1-fimbriated escherichia coli o6:k13:h1 and another group with the same e. coli strain together with l. plantarum 299v. one and 5 weeks after colonization, bacterial numbers were determined in the contents of the small intestine, caecum and mesenteric lymph nodes. small intestinal sections were examined for cd8+, cd4+ ...199910337020
the influence of bacterial inoculants on the microbial ecology of aerobic spoilage of barley silage.the aerobic decomposition of barley silage treated with two inoculants (laca and lacb) containing mixtures of lactobacillus plantarum and enterococcus faecium was investigated over a 28-day period. initially, yeast and bacterial populations were larger in silage inoculated with laca than in silage treated with lacb or water alone (control). differences in the succession of yeasts in silage treated with laca were observed relative to the other two treatments. from silage treatment with laca, issa ...199910349723
arginine dihydrolase pathway in lactobacillus plantarum from orange.lactobacillus plantarum n8 and n4 strains isolated from orange degraded l-arginine to citrulline, ornithine and ammonia. citrulline and ornithine were consumed. lactobacillus plantarum n4 utilized arginine and ornithine to a higher extent than lactobacillus plantarum n8. urea was not detected during arginine degradation, indicating that the amino acid degradation was carried out only by the arginine dihydrolase pathway. citrulline increased the growth of the two strains, arginine only increased ...199910359490
sol-gel composite films with controlled release of biocides.the release of biocides (benzoic, sorbic and boric acids) incorporated into modified silica films was investigated with respect to composite structure. the liberation rates of the embedded acids are proportional to the biocide-to-silica ratio and are changed by adding soluble polymers such as hydroxypropylcellulose. the rates of liberation correlate with biocidal activity, i.e., the growth of microorganisms such as escherichia coli, lactobacillus plantarum and penicillium sp. is strongly suppres ...199910370171
purification and partial amino acid sequence of plantaricin 1.25 alpha and 1.25 beta, two bacteriocins produced by lactobacillus plantarum tmw1.25.two bacteriocins produced by lactobacillus plantarum tmw1.25 have been purified by a four-step purification procedure, including ammonium sulphate precipitation and cation-exchange chromatography followed by hydrophobic-interaction chromatography on octyl sepharose. the final purification was performed by repeated reversed-phase chromatography steps which yielded two bacteriocin fractions designated plantaricin 1.25 alpha and plantaricin 1.25 beta. the molecular masses of the peptides in these f ...199910389253
the oxidized (3,3) state of manganese catalase. comparison of enzymes from thermus thermophilus and lactobacillus plantarum.manganese catalases contain a binuclear manganese cluster that catalyzes the redox dismutation of hydrogen peroxide, interconverting between dimanganese(ii) [(2,2)] and dimanganese(iii) [(3,3)] oxidation states during turnover. we have investigated the oxidized (3,3) states of the homologous enzymes from thermus thermophilus and lactobacillus plantarum using a combination of optical absorption, cd, mcd, and epr spectroscopies as sensitive probes of the electronic structure and protein environmen ...199910413487
duplication of the beta-galactosidase gene in some lactobacillus plantarum strains.curing of a plasmid that encoded a beta-galactosidase gene (beta-gal) from the lactobacillus plantarum strain of dairy origin ll441 was not accompanied by complete loss of the lactose utilization phenotype. dna-dna hybridization, using an internal fragment of the beta-gal gene as a probe, revealed a second determinant located on the chromosome of the cured derivatives. the chromosomal copy was present in all of a series of beta-gal+ l. plantarum and lactobacillus pentosus strains from different ...199910426447
characterization of muscle sarcoplasmic and myofibrillar protein hydrolysis caused by lactobacillus plantarum.strains of lactobacillus plantarum originally isolated from sausages were screened for proteinase and aminopeptidase activities toward synthetic substrates; on the basis of that screening, l. plantarum crl 681 was selected for further assays on muscle proteins. the activities of whole cells, cell extracts (ce), and a combination of both on sarcoplasmic and myofibrillar protein extracts were determined by protein, peptide, and free-amino-acid analyses. proteinase from whole cells initiated the hy ...199910427046
processing of extracellular lipase of lactobacillus plantarum: involvement of a metalloprotease.the hypothesis of a proteolytic involvement in the extracellular lipase processing of a strain of lactobacillus plantarum was considered and tested, in vitro assays with acid proteases, cathepsin d and renin revealed that both did affect lipolytic activity positively. in vivo assays with growth in the presence of the protease inhibitors pepstatin, phenylmethylsulfonyl fluoride, transepoxysuccinyl-l-leucylamido-(4-guanidino)butane and ethylenediaminetetraacetic acid showed that ethylenediaminetet ...199910427731
transport of d-xylose in lactobacillus pentosus, lactobacillus casei, and lactobacillus plantarum: evidence for a mechanism of facilitated diffusion via the phosphoenolpyruvate:mannose phosphotransferase system.we have identified and characterized the d-xylose transport system of lactobacillus pentosus. uptake of d-xylose was not driven by the proton motive force generated by malolactic fermentation and required d-xylose metabolism. the kinetics of d-xylose transport were indicative of a low-affinity facilitated-diffusion system with an apparent k(m) of 8.5 mm and a v(max) of 23 nmol min(-1) mg of dry weight(-1). in two mutants of l. pentosus defective in the phosphoenolpyruvate:mannose phosphotransfer ...199910438743
complementary and overlapping selectivity of the two-peptide bacteriocins plantaricin ef and jk.plantaricin ef and jk are both two-peptide bacteriocins produced by lactobacillus plantarum c11. the mechanism of plantaricin ef and jk action was studied on l. plantarum 965 cells. both plantaricins form pores in the membranes of target cells and dissipate the transmembrane electrical potential (deltapsi) and ph gradient (deltaph). the plantaricin ef pores efficiently conduct small monovalent cations, but conductivity for anions is low or absent. plantaricin jk pores show high conductivity for ...199910438754
detection and characterization of a novel antibacterial substance produced by lactobacillus plantarum st 31 isolated from sourdough.lactobacillus plantarum st31 isolated from sourdough produced an antimicrobial substance inhibiting other strains of the genera lactobacillus, leuconostoc, pediococcus, streptococcus, bacillus and some foodborne pathogens including staphylococcus aureus. this antimicrobial substance was inactivated by proteolytic enzymes. consequently, it was characterized as a bacteriocin and was designated plantaricin st31. this bacteriocin was stable in the ph range 3-8 and it was not affected by amylolytic e ...199910443536
synergistic actions of nisin, sublethal ultrahigh pressure, and reduced temperature on bacteria and yeast.nisin in combination with ultrahigh-pressure treatment (uhp) showed strong synergistic effects against lactobacillus plantarum and escherichia coli at reduced temperatures (<15 degrees c). the strongest inactivation effects were observed when nisin was present during pressure treatment and in the recovery medium. elimination (>6-log reductions) of l. plantarum was achieved at 10 degrees c with synergistic combinations of 0.5 microg of nisin per ml at 150 mpa and 0.1 microg of nisin per ml at 200 ...199910473428
collagen (cn-i) binding by gut lactobacilli.four gut lactobacillus strains displaying the features which make them particularly promising for the preparation of probiotic products were investigated together with 5 fresh isolates and one collection strain of lactobacillus plantarum for their ability to bind type i collagen (cn-i). immobilised cn-i in microtitre plates was bound only by 3 strains of gut lactobacilli from piglets and the collection strain lactobacillus plantarum lhi 10 from prague in range of a570nm readings 0.114-0.221. six ...199910486988
solution structure of plantaricin c, a novel lantibiotic.plantaricin c, a bacteriocin produced by a lactobacillus plantarum strain of dairy origin, is a lantibiotic. one dehydroalanine, one lanthionine and three beta-methyl-lanthionine residues were found in its 27 amino acid sequence. the plantaricin c structure has two parts: the first comprises the six nh2-terminal residues, four of which are lysines, which confer a strong positive charge to this stretch. the amino acids in positions 7 and 27 form the lanthionine bridge, giving a globular conformat ...199910491130
heat resistance of lactobacillus spp. isolated from cheddar cheese.mesophilic lactobacillus spp. are the dominant organisms in mature cheddar cheese. the heat resistance of broth grown cultures of lactobacillus plantarum dpc1919 at temperatures between 50 and 57.5 degrees c, lact. plantarum dpc2102 at temperatures between 48 and 56 degrees c and lact. paracasei dpc2103 at temperatures between 50 and 67.5 degrees c was determined. the z-values for lact. plantarum dpc1919, lact. plantarum dpc2102 and lact. paracasei dpc2103 were 6.7 degrees c, 6.2 degrees c and 5 ...199910499302
a study on growth characteristics and nutrient consumption of lactobacillus plantarum in a-stat culture.lactobacillus plantarum was grown in complex media containing glucose and yeast extract. the maximum growth yield based on yeast extract consumption was 0.5 g dwt g-1. growth yield yatp 15-17 g dwt mol atp-1 was almost constant in the glucose limited a-stat experiment whereas in the yeast extract limited culture it increased with dilution rate. the maximum specific growth rate observed, 0.5 h-1, was similar for both a-stat and batch cultures. specific oxygen consumption, qo2, reached the value o ...199910510718
bile salt deconjugation by lactobacillus plantarum 80 and its implication for bacterial toxicitythe effects of bile salts on the survival of lactobacilli were investigated using glycocholic acid, cholic acid and deoxycholic acid as model compounds and the bile salt hydrolase active lactobacillus plantarum 80 (bsh+) and its bsh negative mutant. the detrimental effects of cholic acid, i.e. growth inhibition and cytotoxicity at a concentration of 1 and 5 mmol l-1, respectively, were considered to be due to the hydrophobic protonated form of the molecule, which brings about membrane damage. th ...199910540235
characterization of cadmium uptake in lactobacillus plantarum and isolation of cadmium and manganese uptake mutants.two different cd(2+) uptake systems were identified in lactobacillus plantarum. one is a high-affinity, high-velocity mn(2+) uptake system which also takes up cd(2+) and is induced by mn(2+) starvation. the calculated k(m) and v(max) are 0.26 microm and 3.6 micromol g of dry cell(-1) min(-1), respectively. unlike mn(2+) uptake, which is facilitated by citrate and related tricarboxylic acids, cd(2+) uptake is weakly inhibited by citrate. cd(2+) and mn(2+) are competitive inhibitors of each other, ...199910543780
cloning, expression, and characterization of cadmium and manganese uptake genes from lactobacillus plantarum.an mn(2+) and cd(2+) uptake gene, mnta, was cloned from lactobacillus plantarum atcc 14917 into escherichia coli. its expression conferred on e. coli cells increased cd(2+) sensitivity as well as energy-dependent cd(2+) uptake activity. both transcription and translation of mnta were induced by mn(2+) starvation in l. plantarum, as indicated by reverse transcriptase pcr and immunoblotting. two cd(2+) uptake systems have been identified in l. plantarum: one is a high-affinity mn(2+) and cd(2+) up ...199910543781
contribution of the microbial and meat endogenous enzymes to the free amino acid and amine contents of dry fermented sausages.the role of the starter culture and meat endogenous enzymes on the free amino acid and amine contents of dry fermented sausages was studied. five batches of sausages were prepared. the control batch was manufactured with aseptic ingredients without microbial inoculation. the other four experimental batches were manufactured with aseptic ingredients inoculated with lactobacillus plantarum 4045 or micrococcus-12 or l. plantarum 4045 and micrococcus-12 or l. plantarum 4045 and staphylococcus sp. th ...199910552431
pesticides in fermentative processes of wine.the influence of six fungicides (azoxystrobin, cyprodinil, fludioxonil, mepanipyrim, pyrimethanil, and tetraconazole) on the fermentative activity of two yeasts (saccharomyces cerevisiae and kloeckeraapiculata) and two lactic bacteria (leuconostoc oenos and lactobacillus plantarum) was studied. the possibility of their being degraded by these yeasts and bacteria was also investigated. the presence of the pesticides did not affect alcoholic fermentation, not even with levels higher than those nor ...199910552734
characterisation of lactic acid bacteria from spoiled, vacuum-packaged, cold-smoked rainbow trout using ribotyping.a total of 405 lactic acid bacteria (lab) isolated from spoiled, vacuum-packaged, salted, sodium nitrite- or potassium nitrate-treated, cold-smoked rainbow trout stored at 4 degrees c or 8 degrees c were characterised and identified using a molecular method. the isolates were initially classified according to their restriction endonuclease profiles using hindiii and ecori restriction endonucleases and further characterised by rrna gene restriction patterns (ribotypes). numerical analysis of thes ...199910573394
effects of water activity, nacl and smoke concentrations on the growth of lactobacillus plantarum atcc 12315.the effect of water activity (a(w)), nacl and liquid smoke concentrations on the growth of lactobacillus plantarum, was studied in mrs broth under anaerobic conditions. a complete 2(3) factorial design was used to determine influential factors and interactions among these factors. nacl concentration and a(w) had a major effect on the maximum biomass obtained but no interaction influenced this response. smoke did not affect either biomass nor acidifying capacities.199910573397
establishment of conditions for green table olive fermentation at low temperature.four lactobacillus plantarum strains were isolated from table olive cold fermentation brines. their specific growth rate and acidification in mrs broth and in green table olive brines were studied by means of a mixed 2 ph (4.5 and 5.0) x 3 salt (3, 4 and 5%, w/v, nacl) x 3 incubation temperature (9, 12 and 15 degrees c) levels factorial design. in mrs broth, the greatest effect (linear) on acidification was due to temperature. in brine, the effects were considerably less, ph (linear) being the m ...199910574089
in vitro activities of ketolide hmr3647, macrolides, and other antibiotics against lactobacillus, leuconostoc, and pediococcus isolates.testing of susceptibility to 13 antibiotics was performed with 90 isolates of lactobacillus, leuconostoc, and pediococcus. mics at which 90% of the isolates tested were inhibited by hmr3647, erythromycin, and ciprofloxacin were 0.015, 0.125 and 32 microg/ml, respectively. the penicillin mic was > or = 16 microg/ml against 26.2% of the studied lactobacillus sp. isolates and 50% of lactobacillus plantarum. hmr3647 showed excellent activity against these genera.199910582908
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