Publications

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the role of nisin in fuel ethanol production with saccharomyces cerevisiae.to investigate the effects of nisin on lactobacilli contamination of yeast during ethanol fermentation and to determine the appropriate concentration required to control the growth of selected lactobacilli in a yp/glucose media fermentation model.201222691226
investigation of asymmetric alcohol dehydrogenase (adh) reduction of acetophenone derivatives: effect of charge density.in an effort to study the effect of substituent groups of the substrate on the alcohol dehydrogenase (adh) reductions of aryl-alkyl ketones, several derivatives of acetophenone have been evaluated against adhs from lactobacillus brevis (lb) and thermoanaerobacter sp. (t). interestingly, ketones with non-demanding (neutral) para-substituents were reduced to secondary alcohols by these enzymes in enantiomerically pure form whereas those with demanding (ionizable) substituents could not be reduced. ...201222609978
biochemical, microbiological and phytochemical studies on aqueous-fermented extracts from atropa belladonna l. part 1--biochemistry and microbiology.extraction methods of fresh plants into aqueous-fermented extracts according to german homoeopathic pharmacopoeia (hab), regulation nos. 33 and 34 were evaluated. in the course of production, the extraction is accompanied by fermentation and the resulting preparation is stored for at least 6 months until further processing. the present work aimed at revealing the underlying biochemical reactions during manufacture and storage. in addition, the responsible microorganisms were isolated and identif ...201222570940
insights into the completely annotated genome of lactobacillus buchneri cd034, a strain isolated from stable grass silage.lactobacillus buchneri belongs to the group of heterofermentative lactic acid bacteria and is a common member of the silage microbiome. here we report the completely annotated genomic sequence of l. buchneri cd034, a strain isolated from stable grass silage. the whole genome of l. buchneri cd034 was sequenced on the roche genome sequencer flx platform. it was found to consist of four replicons, a circular chromosome, and three plasmids. the circular chromosome was predicted to encode 2319 protei ...201222465289
c-terminal truncation of glutamate decarboxylase from lactobacillus brevis cgmcc 1306 extends its activity toward near-neutral ph.glutamate decarboxylase (gad) from lactobacillus brevis is a very promising candidate for biosynthesis of gaba and various other bulk chemicals that can be derived from gaba. however, no structure of gad of this origin has been reported to date, which limits enzyme engineering strategy to improve its properties for better use in production of gaba. bacterial gad exhibits an acidic ph optimum and there is often a sharp ph dependence. in the present work, site-directed mutagenesis was performed to ...201222418267
tight controlled expression and secretion of lactobacillus brevis slpa in lactococcus lactis.prokaryotes commonly present outer cell wall structures composed of a crystalline array of proteinaceous subunits, known as surface layers (s-layers). the orf encoding the s-layer protein (slpa) of lactobacillus brevis was cloned into lactococcus lactis under the transcriptional control of the xylose-inducible expression system (xies). slpa was secreted into the extracellular medium, as determined by immunoblotting, and assays on the kinetics of slpa production revealed that repression of the sy ...201222391736
phenotypic and phylogenetic analysis of lactic acid bacteria isolated from forage crops and grasses in the tibetan plateau.a total of 140 lactic acid bacteria (lab) strains were isolated from corn, alfalfa, clover, sainfoin, and indian goosegrass in the tibetan plateau. according to phenotypic and chemotaxonomic characteristics, 16s rdna sequence, and reca gene pcr amplification, these lab isolates were identified as belonging to five genera and nine species. corn contained more lab species than other forage crops. leuconostoc pseudomesenteroides, lactococcus lactis subsp. lactis, lactobacillus brevis, and weissella ...201222367939
aphthous oral ulceration and its successful management by lactobacillus brevis cd2 extract in an adult haemophilic patient. 201222335552
evaluation of lactic acid bacterium from chilli waste as a potential antifungal agent for wood products.the aim of this study was to isolate lactic acid bacteria from chilli waste and evaluate metabolites produced for the ability to arrest wood decay.201222321006
the two-step biotransformation of monosodium glutamate to gaba by lactobacillus brevis growing and resting cells.gama-aminobutyric acid (gaba) is a natural functional amino acid. in the current study, lactobacillus brevis tccc13007, a high gaba-producing strain, was isolated from naturally pickled chinese vegetables. a two-step cellular bioconversion process was established using l. brevis tccc13007 for the production of gaba. first, l. brevis cells were grown anaerobically in 7% monosodium glutamate (msg)-containing medium at an initial ph of 6.0 and a controlled ph of 4.6 for 16 to 66 h; approximately 38 ...201222307498
in situ fermentation dynamics during production of gundruk and khalpi, ethnic fermented vegetable products of the himalayas.gundruk is a fermented leafy vegetable and khalpi is a fermented cucumber product, prepared and consumed in the himalayas. in situ fermentation dynamics during production of gundruk and khalpi was studied. significant increase in population of lactic acid bacteria (lab) was found during first few days of gundruk and khlapi fermentation, respectively. gundruk fermentation was initiated by lactobacillus brevis, pediococcus pentosaceus and finally dominated by lb. plantarum. similarly in khalpi fer ...201022815579
the arginine-deiminase enzymatic system on gingivitis: preliminary pediatric study.aim.: the lactic bacteria are gram-positive microorganisms with coccus or stick shape, which share a number of physiological and biochemical properties. several experimental evidences suggest the possibility of using lactic acid bacterial as a preventive or therapeutic potential approaches, alternative or complementary to prevention or treatment protocols currently followed in several pathological conditions. lactobacillus brevis is able, through the arginine-deiminase activity, to subtract the ...201022238699
proteolysis in model portuguese cheeses: effects of rennet and starter culture.to shed further light onto the mechanisms of proteolysis that prevail throughout ripening of portuguese cheeses, model cheeses were manufactured from bovine milk, following as much as possible traditional manufacture practices - using either animal or plant rennet. the individual role upon proteolysis of two (wild) strains of lactic acid bacteria - viz. lactococcus lactis and lactobacillus brevis, which are normally found to high viable numbers in said cheeses, was also considered, either as sin ...200826065746
lactic acid bacteria in hamei and marcha of north east india.hamei and marcha are mixed dough inocula used as starters for preparation of various indigenous alcoholic beverages in manipur and sikkim in india, respectively. these starters are traditionally prepared from rice with wild herbs and spices. samples of hamei and marcha, collected from manipur and sikkim, respectively, were analysed for lactic acid bacterial composition. the population of lactic acid bacteria (lab) was 6.9 and 7.1 log cfu/g in hamei and marcha, respectively. on the basis of pheno ...200723100653
fermented vegetable juices obtained from celery, parsley and tomato.application of starter cultures in fermented food industry results in obtaining high quality products, standardized sensory properties and allows to shorten a time of production. employment of appropriate starter cultures indices the possibility of develop a new assortment of fermented products which can not be obtain in traditional way. the aim of study was to produce fermented vegetable juices using starter cultures with different quantitative and qualitative composition. parsley, celery and t ...200324757797
evaluation of fourier-transform infrared spectroscopy for data capture in predictive microbiology.chemical changes in the medium, induced by the fermentative species lactobacillus plantarum and lactobacillus brevis and by the enzymatic action of a proteolytic, spoilage species, yarrowia lipolytica, were analysed using fourier-transform i.r. spectroscopy (ftir). changes in the absorbance data over time could be modelled using one of the more current predictive, mathematical models of microbial growth, such as the gompertz equation. moreover, a linear correlation between ftir data (expressed a ...199624415096
interaction between lactic acid bacteria and yeasts in sour-dough using a rheofermentometer.rheofermentometer assays were used to characterize the leavening of sour-doughs produced using species of lactic acid bacteria (lab) and yeasts, alone or in combination. saccharomyces cerevisiae 141 produced the most co2 and ethanol whereas s. exiguus m14 and lactobacillus brevis subsp. lindneri cb1 contributed poorly to leavening and gave sour-doughs without porosity. in comparison with that seen in sour-dough produced with yeast alone, yeast fermentation with heterofermentative lab present was ...199524415009
short communication: purification and properties of a glucose-forming amylase of lactobacillus brevis.an extracellular glucose-forming amylase was produced by lactobacillus brevis isolated from 'kagasok tea'. the enzyme was purified 70-fold and had optimal activity at 55°c and ph 6.5. its k m value for starch was 0.27 mg ml(-1) and its m r was approx. 75,900 da. the activity of the enzyme was enhanced by ca(2+), mg(2+), na(+) or k(+) and inhibited by edta, kcn, citric acid and l-cysteine.199524414921
the sourdough microflora. interactions between lactic acid bacteria and yeasts: metabolism of amino acids.co-culture of lactobacillus brevis subsp. lindneri or l. plantarum with saccharomyces cerevisiae or s. exiguus from sourdough did not modify the yield of the yeasts but gave higher growth rates and final yields of both lactic acid bacteria (lab) than in their respective mono-cultures. co-cultures of l. brevis subsp. lindneri with s. cerevisiae or s. exiguus in a medium without valine or leucine, which are essential for growth of the lab, led to growth of the lab due to excretion of these amino a ...199424421010
use of starter cultures containingstreptococcus diacetilactis, lactobacillus brevis andsaccharomyces cerevisiae for fermenting milk for production of nigeriannono.starter cultures consisting ofstreptococcus diacetilactis, strept. cremoris, lactobacillus brevis andsaccharomyces cerevisiae were tested singly and in mixtures for ability to ferment milk to producenono with organoleptically acceptable qualities. only mixed cultures containing eitherstrept. diacetilactis orstrept. cremoris andl. brevis were suitable. presence of yeast adversely affected either acid formation or diacetyl production.nono containingstrept. diacetilactis was acceptable, even in the ...199224425471
fermentation patterns of forage sorghum ensiled under different environmental conditions.the effects of temperature, aerobic and anaerobic conditions in the silo and plant characteristics [water-soluble carbohydrate (wsc) contents, growing season] on the fermentation characteristics of a tropical forage species, sorghum bicolor cv. sugar-drip, were investigated. silages fermented in oxygen-impermeable bags were well preserved and had low ph (3.7), high lactic acid [72 g kg(-1) dry matter (dm) ≡ 80% of total acids], and low butyric acid (0.12 g kg(-1) dm) and ammonia nitrogen (nh3-n) ...199124424934
lactobacillus kefir sp.nov., a component of the microflora of kefir.about 100 strains of heterofermentative rod-shaped lactic acid bacteria have been isolated from kefir grains and drink kefir, respectively. these isolates differ from lactobacillus brevis and the other species of heterofermentative lactobacilli so far validly published with respect to biochemical characteristics and dna/dna homology. they are thus considered as representatives of the new species lactobacillus kefir sp. nov., with strain dsm 20587 (= a/k) being the type strain.198323194600
molecular identification of lactobacillus spp. associated with puba, a brazilian fermented cassava food.puba or carimã is a brazilian staple food obtained by spontaneous submerged fermentation of cassava roots. a total of 116 lactobacilli and three cocci isolates from 20 commercial puba samples were recovered on de man, rogosa and sharpe agar (mrs); they were characterized for their antagonistic activity against foodborne pathogens and identified taxonomically by classical and molecular methods. in all samples, lactic acid bacteria were recovered as the dominant microbiota (7.86 ± 0.41 log10 cfu/g ...201324159278
lactobacilli-lactoferrin interplay in chlamydia trachomatis infection.in the cervicovaginal microenvironment, lactobacilli are known to protect against genital infections and, amongst the host defence compounds, lactoferrin has recently acquired importance for its anti-microbial and anti-inflammatory properties. an abnormal genital microenvironment facilitates the acquisition of pathogens like chlamydia trachomatis, the leading cause of bacterial sexually transmitted infections worldwide. the aim of our study is to investigate the effects of lactobacillus crispatu ...201728505248
probiotic-mediated blueberry (vaccinium corymbosum l.) fruit fermentation to yield functionalized products for augmented antibacterial and antioxidant activity.the aim of this study was to investigate the fermentation of blueberry fruit with selected probiotic bacteria (bacillus amyloliquefaciens and lactobacillus brevis) and yeast (starmerella bombicola) isolated from fermented starfish for the extraction of functionalized products for biomedical applications. all probiotic-based fermented extracts showed augmented antibacterial and antioxidant activity compared to the control. biochemical parameters of viable cell count, titratable acidity, total phe ...201728690159
impact of thistle rennet from carlina acanthifolia all. subsp. acanthifolia on bacterial diversity and dynamics of a specialty italian raw ewes' milk cheese.caciofiore della sibilla is an italian specialty soft cheese manufactured with sopravissana raw ewes' milk and thistle rennet prepared with young fresh leaves and stems of carlina acanthifolia all. subsp. acanthifolia, according to an ancient tradition deeply rooted in the territory of origin (mountainous hinterland of the marche region, central italy). in this study, the impact of thistle rennet on the bacterial dynamics and diversity of caciofiore della sibilla cheese was investigated by apply ...201728558331
comparison of acyclovir and multistrain lactobacillus brevis in women with recurrent genital herpes infections: a double-blind, randomized, controlled study.we performed a randomized double-blind controlled trial to compare the efficacy and safety of multistrain probiotic and acyclovir in women patients with recurrent genital herpes simplex virus type 2 (hsv-2) infections. eighty-one patients enrolled in the study were being treated with multistrain lactobacillus brevis one vaginal capsule every 12 h and oral acyclovir 400 mg twice daily for 6 months. of 53 patients who completed both treatment courses, no important differences were identified betwe ...201728852985
complete genome sequence of the sourdough isolate lactobacillus zymae aca-dc 3411.lactobacillus zymae is a gram-positive lactic acid bacterium belonging to the lactobacillus brevis clade. here, we report the first complete genome sequence of l. zymae aca-dc 3411, which was isolated from traditional greek wheat sourdough. whole-genome analysis may reveal adaptive traits of strain aca-dc 3411 in the sourdough ecosystem.201728751400
microbial production of mannitol by lactobacillus brevis 3-a5 from concentrated extract of jerusalem artichoke tubers.in the present study, the conversion of the extract of jerusalem artichoke tubers for mannitol production by lactobacillus brevis 3-a5 was investigated. when the bacterium utilized enzymatic hydrolysates of jerusalem artichoke extract as the main substrates in batch fermentation, the significant decrease in mannitol productivity was observed when the initial concentration of reducing sugar increased. then, a strategy of continuous fed-batch fermentation was adopted for improving mannitol product ...201728833484
a genetically encoded tool for manipulation of nadp(+)/nadph in living cells.the redox coenzymes nadh and nadph are broadly required for energy metabolism, biosynthesis and detoxification. despite detailed knowledge of specific enzymes and pathways that utilize these coenzymes, a holistic understanding of the regulation and compartmentalization of nadh- and nadph-dependent pathways is lacking, partly because of a lack of tools with which to investigate these processes in living cells. we have previously reported the use of the naturally occurring lactobacillus brevis h2o ...201728805804
self-cycling fermentation for 1,3-propanediol production: comparative evaluation of metabolite flux in cell recycling, simple batch and continuous processes using lactobacillus brevis n1e9.3.3 strain.the microbial conversion of biodiesel derived crude glycerol to 1,3-propanediol (1,3-pdo) has attained high industrial value due to the broad range of applications as a monomer (1,3-pdo) in textile, cosmetic and polymer industries. this fine chemical through biological production addressed several limitations of the chemical process like high temperatures, pressure and expensive catalysts. in this study 1,3-propanediol production was achieved from a non-pathogenic lactic acid bacterial strain la ...201728760443
in vitro evaluation of the probiotic and safety properties of bacteriocinogenic and non-bacteriocinogenic lactic acid bacteria from the intestines of nile tilapia and common carp for their use as probiotics in aquaculture.in this study, seven bacteriocinogenic and non-bacteriocinogenic lab strains previously isolated from the intestines of nile tilapia and common carp and that showed potent antibacterial activity against host-derived and non-host-derived fish pathogens were assayed for their probiotic and safety properties so as to select promising candidates for in vivo application as probiotic in aquaculture. all the strains were investigated for acid and bile tolerances, transit tolerance in simulated gastroin ...201728752423
chemoenzymatic synthesis of statine side chain building blocks and application in the total synthesis of the cholesterol-lowering compound solistatin.the synthesis and enzymatic reduction of several 6-substituted dioxohexanoates are presented. two-step syntheses of tert-butyl 6-bromo-3,5-dioxohexanoate and the corresponding 6-hydroxy compound have been achieved in 89% and 59% yield, respectively. regio- and enantioselective reduction of these diketones and of the 6-chloro derivative with alcohol dehydrogenase from lactobacillus brevis (lbadh) gave the (5s)-5-hydroxy-3-oxo products with enantiomeric excesses of 91%, 98.4%, and >99.5%, respecti ...201728751276
multiple genome sequences of exopolysaccharide-producing, brewery-associated lactobacillus brevis strains.lactobacillus brevis represents one of the most relevant beer-spoiling bacteria. besides strains causing turbidity and off flavors upon growth and metabolite formation, this species also comprises strains that produce exopolysaccharides (epss), which increase the viscosity of beer. here, we report the complete genome sequences of three eps-producing, brewery-associated l. brevis strains.201728663299
development of a propidium monoazide-polymerase chain reaction assay for detection of viable lactobacillus brevis in beer.the spoilage of beer by bacteria is of great concern to the brewer as this can lead to turbidity and abnormal flavors. the polymerase chain reaction (pcr) method for detection of beer-spoilage bacteria is highly specific and provides results much faster than traditional microbiology techniques. however, one of the drawbacks is the inability to differentiate between live and dead cells. in this paper, the combination of propidium monoazide (pma) pretreatment and conventional pcr had been describe ...201728633981
anti-inflammatory potential of a heat-killed lactobacillus strain isolated from kimchi on house dust mite-induced atopic dermatitis in nc/nga mice.atopic dermatitis (ad) is an allergic skin disease driven by the th2-prone immune response. therefore, a fundamental approach to restoring the th1/th2 balance is needed to treat ad.201728632956
effect of ensiling and silage additives on biogas production and microbial community dynamics during anaerobic digestion of switchgrass.silage processing has a crucial positive impact on the methane yield of anaerobic treated substrates. changes in the characteristics of switchgrass after ensiling with different additives and their effects on methane production and microbial community changes during anaerobic digestion were investigated. after ensiling (ck), methane yield was increased by 33.59% relative to that of fresh switchgrass (fs). in comparison with the ck treatment, methane production was improved by 17.41%, 13.08% and ...201728577484
clinical and microbiological effects of the adjunctive use of probiotics in the treatment of gingivitis: a randomized controlled clinical trial.to evaluate the efficacy of a probiotic combination in the treatment of gingivitis and to assess its impact on the subgingival microbiota.201728556062
three-dimensional structures and functional studies of two gh43 arabinofuranosidases from weissella sp. strain 142 and lactobacillus brevis.arabinofuranosidases degrade arabinose-containing oligo and polysaccharides, releasing l-arabinose, which is a potentially useful sugar, shown to reduce glycemic response under certain conditions. arabinofuranosidases (arafs) are frequently found in gh43, one of the most common gh-families encoded in genomes in gut microbiota, and hence it is of interest to increase understanding of the function of these enzymes in species occurring in the gut. here we have produced, characterized and solved the ...201728485897
lactic acid bacteria population dynamics during spontaneous fermentation of radish (raphanus sativus l.) roots in brine.the aim of the present study was to assess the microecosystem development and the dynamics of the lactic acid bacteria population during spontaneous fermentation of radish (raphanus sativus l.) roots in brine at 20 and 30 °c. in both temperatures, lactic acid bacteria prevailed the fermentation; as a result, the ph value was reduced to ca. 3.6 and total titrable acidity increased to ca. 0.4% lactic acid. enterococci population increased and formed a secondary microbiota while pseudomonads, enter ...201728466306
thermal treatment of lupin-based milk alternatives - impact on lupin proteins and the network of respective lupin-based yogurt alternatives.in this study lupin-based milk alternative (lbma), obtained from protein isolate of lupinus angustifolius cv. boregine, was homogenized and was subjected to different heat treatments (unheated; pasteurized: 80°c, 60s; ultra-high temperature heating (uht): 140°c, 10s). upon thermal treatment, lupin proteins, namely β-conglutin and α-conglutin, were stepwise denatured, which was accompanied by the exposure of masked sulfhydryl groups, the decrease of free sulfhydryl groups and reoxidation to disul ...201628460988
effect of combined consumption of lactobacillus brevis kb290 and β-carotene on minor diarrhoea-predominant irritable bowel syndrome-like symptoms in healthy subjects: a randomised, double-blind, placebo-controlled, parallel-group trial.people with non-pathological diarrhoea-predominant irritable bowel syndrome (ibs-d)-like symptoms are present at a significant level even in healthy populations, but established prophylaxis is lacking. previously, we have found that co-administration of lactobacillus brevis kb290 (kb290) and β-carotene (βc) attenuated murine colitis (potential cause of ibs-d-like symptoms) significantly. here, we investigated the effect of kb290 and βc on minor ibs-d-like symptoms in healthy volunteers. after a ...201728391736
lactobacillus brevis cd2 lozenges prevent oral mucositis in patients undergoing high dose chemotherapy followed by haematopoietic stem cell transplantation.oral mucositis is a common inflammatory complication in patients undergoing high-dose chemotherapy and radiation followed by haematopoietic stem cell transplantation (hsct). lactobacillus brevis cd2 has been proven efficacious in preventing chemoradiotherapy-induced oral mucositis in squamous cell carcinoma of head and neck.201628848667
correction to: fuke, aizawa, suganuma, takagi, and naito, effect of combined consumption of lactobacillus brevis kb290 and β-carotene on minor diarrhoea-predominant irritable bowel syndrome-like symptoms in healthy subjects: a randomised, double-blind, placebo-controlled, parallel-group trial. 201728817999
the oxygen-inducible conversion of lactate to acetate in heterofermentative lactobacillus brevis atcc367.lactobacillus brevis is an obligatory heterofermentative lactic acid bacterium that produces high levels of acetate which improves the aerobic stability of silages against deterioration by yeasts and molds. however, the mechanism involved in acetate accumulation has yet to be understood. here, experimental evidence indicated that aerobiosis resulted in the conversion of lactate to acetate after glucose exhaustion in l. brevis atcc367 (genbank accession number nc_008497). to elucidate the convers ...201728842545
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