| regulation of cyclopropane fatty acid biosynthesis by variations in enzyme activities. | | 1975 | 1120076 |
| characterization of group n streptococcus lipoteichoic acid. | lipoteichoic acid was extracted from the group n organism streptococcus lactis atcc 9936 with hot aqueous phenol and purified by gel chromatography followed by affinity chromatography using ricinus communis lectin as the specific absorbent. the teichoic acid moiety of the lipoteichoic acid was calculated to contain 16 to 17 glycerol phosphate units, approximately half of which were substituted with alpha-d-galactosyl residues; the glycolipid moiety contained o-alpha-d-glucosyl-1 yields 2-o-alpha ... | 1975 | 1123256 |
| assay of therapeutic doses of methotrexate in body fluids of patients with psoriasis. | a microbiologic technique for the assay of methotrexate (mtx) in urine, serum, erythrocytes, feces, and skin is described. the accuracy of the method equals that of routine microbiologic assays of folic acid. important differences in serum mtx levels in psoriatic patients during the 24 hours after standardized intravenous and intramuscular administration were demonstrated. repeated intravenous doses tended to be cleared from the blood univormly. after oral doses many patients achieved peak serum ... | 1975 | 1123553 |
| heterogeneous elevation of amino acid transport rates in pantothenate-and lipid-deficient lactobacillus plantarum. | the effect of a pantothenic acid deficiency in lactobacillus plantarum on the initial rate of amino acid transport was investigated. although the steady-state accumulation capacity for all amino acids was markedly reduced in pantothenate-deficient cells, initial rates of uptake either were not changed (asparagine, alanine, lysine) or were increased (glutamic acid, aspartic acid leucine). the findings suggest that a reduction in membrane lipid content heterogeneously affects the operation and/or ... | 1975 | 1125249 |
| production and mode of action of lactocin 27: bacteriocin from a homofermentative lactobacillus. | lactobacillus helveticus strain lp27 produced a bacteriocin, lactocin 27, in dialyzable and nondialyzable forms. no evidence was obtained to indicate that lactocin 27 was under the control of extrachromosomal plasmids. lactocin 27 had a bacteriostatic effect on the indicator, lactobacillus helveticus strain ls18. it inhibited primarily protein synthesis without affecting deoxyribonucleic acid and ribonucleic acid synthesis or adenosine 5'-triphosphate levels. treatment of susceptible cells with ... | 1975 | 1137365 |
| effects of high risk and low risk diets for colon carcinogenesis on fecal microflora and steroids in man. | we investigated the effects of a high meat mixed western diet and a nonmeat diet, representing the dietary pattern of high and low risk areas for colon cancer, respectively, on fecal microflora dn on bile acid and neutral sterol patterns in man. the total anaerobic microflora as well as the count of bacteroides, bifidobacterium, peptococcus, and anaerobic lactobacillus were significantly higher during the period of consumption of a high meat mixed western diet comparted with the nonmeat-diet con ... | 1975 | 1138032 |
| oxygen metabolism of catalase-negative and catalase-positive strains of lactobacillus plantarum. | two catalase-negative strains of lactobacillus plantarum and a strain producing the atypical, nonheme catalase were studied to determine if the ability to produce the atypical catalase conferred any growth advantage upon the producing strain. both catalase-negative strains grew more rapidly than the catalase-positive strain under aerobic or anaerobic conditions in a glucose-containing, complex medium. upon exhaustion of glucose from the medium, all three strains continued growth under aerobic bu ... | 1975 | 1141195 |
| deterioration of high-moisture corn. | two small, leaky silos were filled with normal high-moisture corn (hmc), and two with hmc severely infested by helminthosporium maydis. counts of mesophilic bacteria, lactobacilli, coliforms, yeasts, and molds were made on corn samples as received and periodically thereafter during 220 days of storage. temperature and gas levels also were monitored. sequential changes in the populations of lactobacilli, yeasts, and molds were determined during spoilage of hmc. these population changes were compa ... | 1975 | 1147609 |
| antibiotic-disc tests for rapid identification of non-sporing anaerobes. | the sensitivity patterns to a number of antibiotic-impregnated discs of 126 strains of gram-negative anaerobes and 36 strains of gram-positive non-sporing anaerobes from various sources have been examined. the sensitivity patterns shown by bacteroids fragilis and bacteroides melaninogenicus were useful for the rapid identification of these organisms. consistent and useful sensitivity patterns were not shown by the gram-positive anaerobes. | 1975 | 1150890 |
| extraction of serum vitamin b12 for radio-isotopic and lactobacillus leichmannii assay. | the protein precipitates discarded during the extraction process of the lactobacillus leichmannii vitamin b12 assay have been shown to contain significant amounts of vitamin b12. this loss of vitamin b12 provide a satisfactory explanation for many of the descrepancies between the serum vitamin b12 values obtained by the l. leichmannii method and the radio-isotopic method of raven et al (1969). it is possible to produce lower results by the method of raven et al (1969)by incorporating into that m ... | 1975 | 1150894 |
| high nicotinic acid content in two ethiopian sorghum lines. | | 1975 | 1151006 |
| effect of actinomycin d and chloramphenicol on the induction of the malate decarboxylase system in lactobacillus thermobacterium 5 (cnrz) 313. | this paper presents data on the induction of malate decarboxylase in lactobacillus thermobacterium and the effect of chloramphenicol and actinomycin d on the induction. | 1975 | 1151345 |
| formation of extracellular lipoteichoic acid by oral streptococci and lactobacilli. | examination of the culture fluids from a number of strains of oral streptococci and latobacilli has shown the presence of an erythrocyte-sensitizing antigen with the properties of lipoteichoic acid. the antigen was isolated from the culture fluids of lactobacillus casei and lactobacillus fermentum and characterized chemically and serologically, for other strains, serological evidence for the presence of lipoteichoic acid depends on the reactivity with antiserum specific for the glycerol phosphat ... | 1975 | 1158524 |
| effect of inhibition of protein synthesis on lipid metabolism in lactobacillus plantarum. | in lactobacillus plantarum 17-5, lipid synthesis appears to be correlated with protein synthesis. inhibition of protein synthesis by chloramphenicol (50 mug/ml) caused the nearly simultaneous inhibition of incorporation of radioactive oleic acid into polar lipids before the cessation of growth. in addition, de novo fatty acid synthesis, as determined by the incorporation of radioactive acetate into cellular lipids, was also inhibited. removal of the antibiotic resulted in the resumption of growt ... | 1975 | 1158852 |
| synergism between trichuris suis and the microbial flora of the large intestine causing dysentery in pigs. | the role of the microbial flora of the large intestine in experimental trichuris suis infection was studied by comparing the clinical syndrome in conventionally reared (cr) pigs, specific pathogen-free pigs, and gnotobiotic pigs. thedisease in cr pigs was characterized by a severe mucohemorrhagic enteritis; in contrast, a mild catarrhal enteritis was observed in specific pathogen-free and gnotobiotic pigs. spirochaetes and vibrio-like organisms were observed only in cr pigs and increased during ... | 1975 | 1167536 |
| quantitative method for the gas chromatographic analysis of short-chain monocarboxylic and dicarboxylic acids in fermentation media. | a method for the preparation and gas chromatographic analysis of the butyl esters of volatile (c-1-c-7) and nonvolatile (lactic, succinic, and fumaric) acids in microbial fermentation media is presented. butyl esters were prepared from the dry salts of the acids. the esters were separated by temperature programming on a column of chromosorb w coated with dexsil 300 gc liquid phase and analyzed with a flame ionization detector. apparent recoveries with butanol-hcl or butanol-h2so4 as butylating a ... | 1975 | 1167776 |
| adenosylcobalamin analogues as inhibitors of ribonucleotide reductase and vitamin b12 transport. | | 1975 | 1168310 |
| evaluation of human oral organisms and pathogenic streptococcus for production of iga protease. | iga protease is a proteolytic enzyme found in whole human saliva and in dental plaque that cleaves both secretory and myeloma iga of human origin to yield intact fabalpha and fcalpha fragments. to determine which bacteria are capable of producing this enzyme, we have examined a variety of strains normally found in the human oral cavity and a number of streptococci of known lancefield group serotype. streptococci of groups a, b, c, d, f, g, h, m, and n, streptococcus mutans, streptococcus sanguis ... | 1975 | 1168676 |
| [amine decomposition by enterobacteria]. | | 1975 | 1168790 |
| oral contraceptives, serum folate, and hematologic status. | previous reports have suggested deleterious effects on folate balance in users of contraceptives. a study of folate in 526 women attending a family-planning clinic demonstrated, both with the lactobacillus casei assay and with a new radioassay, that the mean serum folate level for women taking oral contraceptives was not significantly lower than that for women in a control group. there was no correlation between serum folate level and length of time oral contraceptives were taken. also, there wa ... | 1975 | 1172864 |
| the nature of the multiple forms of d-erythrodihydroneopterin triphosphate synthetase. | 1. three forms of the lactobacillus plantarum enzyme d-erythro-dihydroneopterin triphosphate synthetase, the first enzyme in folate biosynthesis, have been demonstrated by polyacrylamide gel electrophoresis. the enzyme forms designated the alpha prime, alpha and beta forms have been shown to be conformers with molecular weights of approx. 200 000. study of the subunit structure of the beta enzyme species by sodium dodecylsulfate-polyacrylamide gel electrophoresis revealed a single protein with a ... | 1975 | 1174547 |
| clinical and laboratory observations on serum folate-binding protein. | we studied the effect of serum folate-binding protein (fbp) on folate radioassays and the relationship of the serum level of unsaturated fbp to the serum folate level in various clinical states. our modification of a heat-extracted radioassay was compared to a whole serum radioassay. our results confirmed the existence of elevated serum levels of unsaturated fbp in some normal subjects, in some women taking oral contraceptives, and in most patients with uremia. elevated levels of unsaturated fbp ... | 1975 | 1174694 |
| trans-n-deoxyribosylase: purification by affinity chromatography and characterization. | trans-n-deoxyribosylase (ec 2.4.2.6) is usually considered as a single protein catalyzing indifferently the transfer of the deoxyribosyl moiety to and from a purine or a pyrimidine base. affinity chromatography of an extract from lactobacillus helveticus with two types of ligands allowed the separation and purification of two distinct trans-n-deoxyribosylases. one catalyzes specifically the deoxyribosyl transfer to and from purine bases exclusively: trans-n-deoxyribosylase-i, the other catalyzes ... | 1975 | 1175596 |
| trans-n-deoxyribosylase: substrate specificity studies. purine bases as acceptors. | a series of purine bases and analogues were tested as substrates for trans-n-deoxyribosylase (ec 2.4.2.6). it was observed that the pyrimidine ring and its substituents on positions 1, 2 and 6, are of minor importance. on the other hand only a few modifications are tolerated on the imidazole moiety, as follows. 1. a tautomeric proton must be present on the imidazole ring. the "usual" shift is between position 9 and 7. 2. the position of the tautomeric proton governs the site of substitution. 3. ... | 1975 | 1175597 |
| lysis of modified walls from lactobacillus fermentum. | the n and o substitution in wall peptidoglycan from lactobacillus fermentum was studied in relation to growth phase, as well as the lytic activities and the effect of trypsin on them. the n-nonsubstituted sites were determined by dinitrophenylation techniques. the results indicate that an extensive substitution at the o groups takes place as cells go into the stationary growth phase, concomitant with a decrease in their lysozyme sensitivity. n-nonsubstituted residues, mainly glucosamine, occurre ... | 1975 | 1176437 |
| ultrastructure of lactobacillus fermentum during early and late growth phases and during thiamine deficiency. | thin sections of exponentially growing and stationary-phase cells of l. fermentum from thiamine sufficient and thiamine deficient media were studied by electron microscopy. compared to the exponential-phase cells the stationary-phase cells from both types of media had thicker cell walls and cross walls and fewer and smaller granules of storage material. exponential-phase, thiamine deficient cells had rather thin cell walls and small mesosomes. | 1975 | 1179752 |
| immunoadjuvant activities of cell walls and their water-soluble fractions prepared from various gram-positive bacteria. | the cell walls from all 21 species of gram-positive bacteria examined, except lysozyme-susceptible micrococcus lysodeikticus (nctc 2665) and lysozyme-resistant staphylococcus epidermidis (atcc 155), were found to be definitely adjuvant-active in both stimulation of increased serum antibody levels and induction of delayed-type hypersensitivity to ovalbumin when administered to guinea-pigs as water-in-oil emulsions. using various cell wall lytic enzymes, the immunoadjuvant principles were solubili ... | 1975 | 1180872 |
| immunoadjuvant activities of peptidoglycan subunits from the cell walls of staphyloccus aureus and lactobacillus plantarum. | attempts were made to isolate and identify the unit chemical structure essential for manifestation of the immunoadjuvant activities characteristic of bacterial cell walls. the n-acetylmuramyl-peptide subunit monomers, nalpha-(n-acetylmuramyl-l-alanyl-d-isoglutaminyl)-nepsilon-(glycylglycyl)-l-lysyl-d-alanine from the cell walls of staphylococcus aureus (fda 209p) and n-acetylmuramyl-l-alanyl-d-isoglutaminyl-meso-diaminopimelic acid and/or n-acetylmuramyl-l-alanyl-d-isoglutaminyl-meso-diaminopime ... | 1975 | 1180873 |
| antitumour glycopeptides from lactobacillus bulgaricus cell wall. | | 1975 | 1181198 |
| the reduction of n-hydroxy-4-acetylaminobiphenyl by the intestinal microflora of the rat. | the role of the intestinal flora in the conversion of n-hydroxy-4-acetyl-aminobiphenyl (n-oh-aabp) to 4-acetylaminobiphenyl has been examined. this reaction, which reverses the metabolic activation of the parent carcinogen, can be demonstrated in cultures of some bacteria indigenous to the intestinal microflora. these include cultures of clostridium sp., clostridium perfringens, peptostreptococcus productus i, and bacteroides fragilis ss. thetaiotaomicron and ss. vulgatus. in contrast, cultures ... | 1975 | 1182690 |
| differential medium for detecting dental plaque bacteria resembling actinomyces viscosus and actinomyces naeslundii. | a medium for detecting colonies of actinomyces viscosus and actinomyces naeslundii in dental plaque samples was developed. the medium (cnac-20) contains 20.0 mug of 3cdso4-8h2o per ml of columbia cna agar base. laboratory strains of a. viscosus grew on cnac-20 in characteristic round, white, smooth, opaque colonies. increasing the cadmium concentration impaired the growth of some a. viscosus strains. stock strains of a. naeslundii and a. israelii grew in colonies of similar white, opaque morphol ... | 1975 | 1184734 |
| isolation and characterization of several glycoproteins from human colostrum whey. | five homogeneous closely related glycoproteins were isolated from human colostrum whey, and their molecular weights and carbohydrate contents ranged from 26 000 to 35 000 and from 50 to 80%, respectively. all had the lactobacillus bifidus var. pennsylvanicus growth-promoting activity, which was not substantially altered by desialization. neither human colostrum, milk or the glycoproteins had any cellular immunity-mediating activity. degradation of the carbohydrate moieties of these glycoproteins ... | 1975 | 1191679 |
| pediococcus acidilactici ncib 6990, a new test organism for microbiological assaying of pantothenic acid. | | 1975 | 1194131 |
| proton magnetic relaxation studies of the interaction of d-xylose and xylitol with d-xylose isomerase. characterization of metal-enzyme-substrate interactions. | the interaction of d-xylose isomerase purified from two sources with mn2+ and d-xylose or the competitive inhibitor xylitol has been examined by nuclear magnetic resonance. a greater paramagnetic effect of enzyme-bound mn2+ on the alpha anomer of d-xylose than on the beta anomer was observed, providing independent evidence for the specificity of d-xylose isomerase for the alpha anomeric form of d-xylose. the exchange rate of alpha-d-xylose into the ternary complex, determined from the normalized ... | 1975 | 1194275 |
| [the fecal flora of man. iii. communication: the composition of lactobacillus flora of different age groups (author's transl)]. | from fecal flora of 66 infants aged 3 to 220 days, 29 children aged 4 to 6 years, 42 adults aged 20 to 64 years and 21 senile persons aged 65 to 86 years, the lactobacillus flora was analysed qualitatively and quantitatively. in the feces of babies only the resident lactobacilli (l. acidophilus, l. salivarius and l. fermenti) were found, while in the feces of children, adults and senile persons the resident lactobacilli as well as the transient lactobacilli (l. casei, l. plantarum, l. brevis, l. ... | 1975 | 1199522 |
| [studies on the determination of tryptophan in pharmaceuticals. an evaluation]. | | 1975 | 1199766 |
| numerical taxonomy and laboratory identification of actinomyces and arachnia and some related bacteria. | a numerical taxonomic study was made on 49 facultative anaerobic gram-positive filamentous and/or diphtheroidal organisms isolated from dental plaques, carious dentin and faeces, together with 63 reference strains belonging to the genera actinomyces, arachnia, bifidobacterium, actinobacterium, propionibacterium, eubacterium and lactobacillus. they were examined for 90 unit characters covering a wide range of tests and properties. the data were subjected to computer analysis in which the simple m ... | 1975 | 1202157 |
| [volatile oil of anethum graveolens l. as an inhibitor of yeast and lactic acid bacteria]. | the antimicrobial activity of 25 volatile oils from aerial parts and seeds of dill (anethum graveolens l.) of different geographical origin towards yeast saccharomyces vini and lactic acid bacteria lactobacterium buchneri was measured by serial dilutions. volatile oils from mature seeds and green parts of the plants harvested at late vegetation phases showed the highest activity. the geographical origin of plants influenced insignificantly the antimicrobial activity of volatile oil. | 1975 | 1208405 |
| [influence of products of thermophilous methane fermentation on the mixed lactic acid bacterial culture]. | the effect of products resulting from thermophilous methane fermentation on the accumulation and ratio of different lactic acid bacteria (str. lactis, str. cremoris, str. diacetilactis, str. diacetil. var. acetoinicus, leuc. citrovorum8 l. plantarum) was investigated upon their combined cultivation in milk and serum. in combined cultivation of the streptococcal culture the rate of growth of a single strain differed from that in separate cultivation. str. cremoris was accumulated with the highest ... | 1975 | 1208408 |
| [lactic acid bacteria in the intestinal tract of useful insects]. | | 1975 | 1209955 |
| anaerobic and aerobic skin bacteria before and after skin-disinfection with chlorhexidine: an experimental study in volunteers. | the amount, composition, and localization of anaerobic and aerobic bacteria in the normal skin before and after disinfection were the subject of a volunteer study. the superficial bacterial flora were sampled by velvet pad imprints, and the deep flora were determined from whole skin biopsies. only one anaerobic species, propionebacterium acnes, was encountered even though other and more strict anaerobic bacteria could have been grown with the anaerobic technique employed. staphylococcus albus do ... | 1975 | 1214012 |
| [use of preparations of flower pollens and lactobacilli]. | | 1975 | 1216524 |
| [study of the antimicrobial effect of higher plants on lactic acid bacteria]. | | 1975 | 1219329 |
| [lactobacillary flora of the animal organism and its protective function]. | | 1975 | 1219332 |
| [bacteriocinogenesis by lactobacillus salivarius, l. jugurti, l. helveticus and l. lactis]. | | 1975 | 1221693 |
| antimetabolites of pantothenic acid, ureido- and carbamoyl-derivatives. | pantothenic acid analogs have been synthesized that contain alkyl and/or arylureido and carbamate functions in the beta-alanyl portion of the amide moiety of the vitamin. the analogs are inhibitory to growth of lactic acid bacteria at concentrations as low as 0.6 mug/ml, and the inhibitions are competitively reversed by supplements of pantothenic acid. the carbamate derivatives are more toxic to growth of lactobacillus plantarum 8014 and pediococcus cerevisiae 8042 than the ureido analogs, and c ... | 1975 | 1228973 |
| studies of the ribosome-associated vitamin b12s adenosylating enzyme of lactobacillus leichmannii. | | 1976 | 1230016 |
| bacteriocinogeny in the genus lactobacillus. | | 1975 | 1230459 |
| [characteristics of an aerobic mutant of lactobacillus bifidus. 3. aerobiosis of lactobacillus bifidus induced by single-cell culture technic]. | | 1975 | 1240195 |
| [electron microscopic observation, isolation and characteristics of spirosome of lactobacillus]. | | 1975 | 1240207 |
| [antigenic characteristics of lactobacilli serologically related to group a and group e]. | | 1975 | 1240319 |
| glycoproteins from mature human milk whey. | two milk-specific glycoprotein fractions and serum orosomucoid were isolated from mature human milk. one of the milk-specific glycoproteins was homogeneous and was termed glycoprotein a. its sedimentation and diffusion constants were 2.6 s and 6.3 f, respectively, giving a molecular weight of 29,5000. it contained some 70% carbohydrate consisting of sialic acid, galactose, fucose, glucosamine, and galactosamine. it was not immunologically related to serum orosomucoid. the other glycoprotein, cal ... | 1976 | 1246459 |
| metabolism of dihydroaminopterin and the influence of aminopterin and related derivatives on the metabolism of (-)-5-formyl-tetrahydrofolic acid in pediococcus cerevisiae. | [3h]dihydroaminopterin, but not [3h]methotrexate or [3h]aminopterin, was rapidly taken up by resting cell of pediococcus cerevisiae and inhibited the uptake of (-)-5-formyl-tetrahydrofolic acid to more than 90% at a concentration of 25 mum. on the other hand 5-formyl-tetrahydrofolic acid inhibited the uptake of dihydroaminopterin to a similar extent. dihydroaminopterin was metabolized by pediococcus cerevisiae, but not by lactobacillus casei, to a compound tentatively identified as dihydroaminop ... | 1976 | 1247588 |
| hydrolysis of urea in the gastrointestinal tract of "monoxenic" rats: effect of immunization with strains of ureolytic bacteria. | axenic rats, in whose feces urea is ordinarily excreted, were inoculated with ureolytic strains of lactobacillus or actinobacillus originally derived from the microflora of "holoxenic" rats. in these "monoxenic" animals, harboring one or another of the bacterial strains, fecal urea was hydrolyzed, with a more rapid onset of ureolysis in the case of actinobacillus as compared with lactobacillus. in vitro, a parallel difference between the two strains with regard to the onset of ureolysis was obse ... | 1976 | 1248879 |
| plasma biotin levels in children with burns and scalds. | the plasma concentrations of biotin were measured for up to 49 days after injury in nine children with burns and scalds which involved from 12% to 50% of the surface area of the body. biotin levels below the minimum of the control range were observed in eight of the nine injured children at some stage during the episode. | 1976 | 1249250 |
| the effect of raney nickel on the covalent thymidylate synthetase-5-fluoro-2'-deoxyuridylate-5,10-methylenetetrahydrofolate complex. | raney nickel (ni(h)) catalyzes a specific reductive cleavage of carbon-sulfur bonds and, therefore, can be used to determine whether compounds are covalently bound to proteins through a sulfide linkage. when the covalent thymidylate synthetase-[3h]5-fluoro-2'-deoxyuridylic acid-[14c]-5,10-ch2h4-folate complex (langenbach et al. (1972a), biochem, biophys. res. commun. 48, 1565) was denatured and then shaken with ni(h) at 25 degrees c, both isotopes were rapidly cleaved from the protein, with iden ... | 1976 | 1252451 |
| phosphopantothenic acid--the major product of pantothenic acid accumulation and a significant growth stimulatn in lactobacillus plantarum. | non-proliferating cells of lactobacillus plantarum accumulated about 1.3 mug of pantothenic acid (pa) per milligram dry cells when placed in a phosphate-buffered solution containing glucose, nacl, and pa for 21 h. under the conditions applied for pa uptake, ph 7.5 and 38 degrees c, the accumulated pa existed almost exclusively in a bound form. free pa was observed only occasionally and then at questionably significant levels. all bound pa was released quantitatively by enzyme hydrolysis with alk ... | 1976 | 1252995 |
| a study of the growth inhibition of lactobacillus casei by n-alkyl homologs of n-nitro-n-nitrosoguanidine. | n-methyl-(mnng), n-ethyl-(enng), and n-propyl-(pnng) derivatives of n'-nitro-n-nitrosoguanidine inhibited the growth of lactobacillus casei in the order of potency, mnng greater than enng greater than pnng. l-cysteine, gluathione, and dithioerythritol reversed the inhibition on a molar basis. the -sh compounds accelerated the loss of the n-nitroso group in vitro, yielding non-inhibitory n-alkyl nitroguanidines. the significance of the loss of the nitroso group and the size of the n-alkyl group i ... | 1976 | 1253336 |
| preparation and partial characterization of a lactobacillus lactis bacteriophage. | high-titer lysates of a bacteriophage active against lactobacillus lactis were prepared from liquid cultures as well as from areas of confluent lysis in soft-agar overlayers. phage concentration and purification were accomplished by means of polyethylene glycol precipitation, differential centrifugation. the buoyant density of this phage in cesium chloride was 1.4795 g/ml. characterization of phage growth cycle by one-step growth experiments under optimal conditions showed that the latent period ... | 1976 | 1259512 |
| the interaction of adeninylalkylcobalamins with ribonucleotide reductase. | several structural analogs of adenosylcobalamin, containing 2, 3, 4, 5 and 6 methylene carbons instead of the ribofuranose moiety, have been synthesized and their interaction with ribonucleotide reductase from lactobacillus leichmannii has been investigated. kinetic studies of the inhibition of the reductase by these analogs showed that the adeninylalkylcobalamins with 4, 5 and 6 carbons interposed between the adenine moiety and the cobalt atom are potent inhibitors of ribonucleotide reduction. ... | 1976 | 1260019 |
| formation of acetaldehyde from threonine by lactic acid bacteria. | group n streptococci were found to cleave threonine to form acetaldehyde and glycine. threonine aldolase, the enzyme catalysing this reaction, was found in all strains except streptococcus cremoris z8, an organism which had been shown previously to have a nutritional requirement for glycine. the enzyme was strongly inhibited by glycine and cysteine. the inhibition showed characteristics of allosteric inhibition and was ph-dependent. inhibition by glycine, but not by cysteine, was highly specific ... | 1976 | 1262564 |
| comparative studies on the microbiological vitamin b12 assay at two laboratories. | the turbidimetric methods in routine use at two laboratories for the microbiological assay of vitamin b12 have been compared. attempts were made to standardize some major parts of the method, i.e., assay design, test strain (lactobacillus leichmannii), test medium, and reference standard. the laboratories used different approaches to achieve efficient assay procedures. during a 6-year period four comparative experiments were carried out. in these experiments the vitamin b12 content of five diffe ... | 1976 | 1267444 |
| determination of the anomeric configuration of d-xylose with d-xylose isomerases. | | 1976 | 1268883 |
| stability of vitamin b12 in the presence of ascorbic acid. | experiments were performed in two independent laboratories, each using their own meal preparations which were exactly similar in composition to the meals described by herbert and jacob (j. am. med. assoc. 230:241, 1974), in order to check their report that incubating meals (portions of daily food intake by man) of "modest" or "high" vitamin b12 content with increasing levels of added l-ascorbic acid (vitamin c) produced increasing destruction of vitamin b12. the present studies were performed wi ... | 1976 | 1274888 |
| transformation of amino acid composition in bacterial cells of lactobacillus bulgaricus during freeze-drying. | | 1976 | 1277861 |
| simplified gram stain interpretive method for diagnosis of bacterial vaginosis. | two methods which only consider bacterial morphotypes and require counting various types of bacteria, for interpreting gram stains of vaginal secretions for the purpose of diagnosing bacterial vaginosis were previously described. a new interpretative method, which combines clue cells with bacterial morphotypes as diagnostic markers, is offered. | 1992 | 1279973 |
| microbiological diagnosis of bacterial vaginosis. | | 1992 | 1282150 |
| irreversible inhibition of thymidylate synthase by pyridoxine (b6) analogues. | three vitamin b6 analogues have been synthesized and tested as inhibitors of thymidylate synthase. the compounds are: 4',5'-dichloro-, 4',5'-dibromo- and 4',5'-diiodo-pyridoxine. all three analogues inhibited the enzyme irreversibly. the kinetic data for the chloro- and bromo-analogues showed that a limiting rate of inhibition is approached as the inhibitor concentration is increased, which indicates that a reversible enzyme:inhibitor affinity complex is formed prior to the irreversible reaction ... | 1992 | 1284431 |
| 1-phenyl-3-trimethylaminopropyl carbodiimide: a new inhibitor of thymidylate synthase. | thymidylate synthase (ec 2.1.1.45) from methotrexate-resistant lactobacillus casei was inactivated by 1-phenyl-3-trimethylaminopropyl carbodiimide (ptc), 1-phenyl-3-dimethyl aminopropyl carbodiimide (pdc), and 1-ethyl-3-dimethyl aminopropyl carbodiimide (edc). in the presence of excess ptc, the inactivation followed pseudo-first order kinetics; the second order rate constant was approximately 200 m-1min-1 at 30 degrees c. the rate of inactivation by ptc was faster than that by either pdc or edc. ... | 1992 | 1285247 |
| antibacterial, antimycotic and trichomonicidal activity of a new nitroimidazole (eu 11100). | the antimicrobial profile of a new nitroimidazole derivative (5-nitro-1-methyl-imidazolyl-2-hydroxy-3 terbutylphenyl carbinol) has been studied. the in vitro activity of the new molecule has been evaluated against both aerobic and anaerobic bacteria, trichomonas vaginalis, and mycetes, under suitable experimental conditions. the new compound was compared with ampicillin against aerobic bacteria; with metronidazole against anaerobic bacteria, lactobacilli and t. vaginalis; with nistatin and econa ... | 1992 | 1287136 |
| changes with time in the oral microflora and dental caries induction in hyposalivated rats fed on sucrose diet. | the effects of hyposalivation on the induction of dental caries and the change in oral microflora were examined at weekly intervals in sprague-dawley rats fed on diet 2000 containing 56% sucrose. in hyposalivated rats, significant dental caries was induced within one week and its severity increased with the experimental period. bacteriological examinations demonstrated that the number of total cultivable microorganisms, lactobacilli and staphylococcus aureus increased significantly shortly after ... | 1992 | 1287402 |
| [lactobacilli, bacterial vaginosis and mycotic colpitis]. | | 1992 | 1291130 |
| in vitro antibacterial activity of antiseptics against vaginal lactobacilli. | the results of investigations carried out to evaluate the inhibitory activity in vitro of seven vaginal antiseptic douche solutions against several strains of vaginal lactobacilli isolated from asymptomatic women are reported. some of the products examined showed marked antibacterial activity even at high dilutions and for short exposure times. the post-antibiotic effect of two of these antiseptics on vaginal lactobacilli was also evaluated. the results of these investigations suggest that uncon ... | 1992 | 1291315 |
| effects of cultured milk products by lactobacillus and bifidobacterium species on the secretion of bile acids in hepatocytes and in rats. | whey preparations prepared from cultured milk by 19 lactobacillus (2 species) and 20 bifidobacterium (5 species) strains were examined for the effects of secretion and synthesis of bile acids in primary cultured rat hepatocytes. the stimulating effect of whey preparation on bile acid secretion depended on the species as well as the strains used for milk fermentation. two strains belonging to l. casei sbt 2230 (lc2230) and b. longum sbt 2912 (bl2912) produced the whey which stimulates both the se ... | 1992 | 1291639 |
| oral sugar clearance and root caries prevalence in rheumatic patients with dry mouth symptoms. | the relationship between root caries, oral sugar clearance, salivary flow rate, and salivary counts of mutans streptococci, lactobacilli and candida has been studied in a group of 22 rheumatic patients (age range 40-72 years). the study group comprised all subjects volunteering for a clinical trial on relief of dry mouth symptoms. the median salivary flow was 0.09 ml/min at rest and 0.9 ml/min during chewing stimulation. the median sugar clearance time was about 5 min in the sublingual area and ... | 1992 | 1294304 |
| prediction of root caries in periodontally treated patients maintained with different fluoride programmes. | the aims of the investigation were to evaluate the effect of different fluoride programmes, as adjuncts to professional plaque control every 3-4 months, on root caries incidence in periodontally treated patients and to identify risk factors for root caries development. ninety-nine individuals, 33-76 years old, who had been treated for periodontal disease were subjected to one of three fluoride programmes during a 2-year period: (1) professional application, 3-4 times/year, of duraphat (n = 34) o ... | 1992 | 1294306 |
| [the distribution of mutans streptococci and lactobacilli depending on the caries prevalence in 11- to 12-year-old children]. | 20 korean children, aged 11 to 12 years, participated in this study with the aim to compare the microbial composition of plaque samples in order to estimate, which genera (lactobacilli, streptococci) or species are associated with coronal caries. significant differences in the microbial composition of plaque samples between children with healthy and carious dentitions were found. s. mutans (c, e, f) was dominating under mutans-streptococci. | 1992 | 1295585 |
| [candida krusei in genital infections]. | frequency of appearance of candida krusei in human reproductive tract, susceptibility of these microorganisms to drugs and their importance in pathogenesis of mycosis, were investigated. strains c. krusei species constituted 6.7% of the total 1325 strains and 21.8% within other than c. albicans species yeast-like fungi. vaginal mycosis caused by fungi from c. krusei species occurs in presence of lactobacillus sp. and correct values of ph in vaginal contents, however changed leukocytosis in evide ... | 1992 | 1297039 |
| [the role of candida kefyr (c. pseudotropicalis) in reproductive tract infections]. | frequency of appearance of c. kefyr strains, their diagnosis, susceptibility to drugs and importance in pathogenesis of reproductive tract mycoses were investigated. the investigated material consisted of 2717 strains, and there were 67 strains of c. kefyr isolated from vaginal mucous membrane. c. kefyr strains constituted 2.5% of total number of 2717 isolated strains and 8.2% within fungi other than c. albicans species. vaginal mycosis caused by c. kefyr occurs in presence of lactobacillus sp. ... | 1992 | 1297040 |
| membrane transport of folate compounds. | all eukaryotic cells and some prokaryotes that are unable to synthesize folic acid utilize membrane-associated transport systems for acquisition of the pre-formed vitamin or its coenzyme forms from external sources. these transport systems, in addition to providing folates essential for cell replication, are also important because of their role in the internalization of antifolates such as methotrexate (mtx) that are used extensively in cancer chemotherapy. information about the components and m ... | 1992 | 1297801 |
| fatal lung abscess due to lactobacillus casei ss rhamnosus. | a fatal case of community acquired pneumonia due to lactobacillus casei ss rhamnosus is reported. clinicians should be aware of this type of pneumonia. | 1992 | 1298232 |
| infection and labor. vii. microbial invasion of the amniotic cavity in spontaneous rupture of membranes at term. | the purpose of this study was to determine the frequency, microbiologic characteristics, and clinical significance of microbial invasion of the amniotic cavity in women with premature rupture of membranes at term. | 1992 | 1301006 |
| [the effect of lactobacillus acidophilus "solco" on the immunological indices of totally decontaminated mice under complete gnotobiological isolation]. | the possibility of stimulating the immunity of totally decontaminated mice, kept isolated under germ-free conditions, with the use of killed l. acidophilus solco strains has been studied. as revealed in this study, the oral and intraperitoneal administration of strains o1 and o6 to mice leads to a significant increase in the content of immunoglobulin-synthesizing cells in the jejunal lamina propria of the animals. the oral administration of l. acidophilus solco strain o6 and the intraperitoneal ... | 1992 | 1301651 |
| characteristics of women by smoking status in the san francisco bay area. | to better understand why smokers are more likely to develop cervical cancer than nonsmokers, we investigated laboratory and demographic differences between the two groups. women between the ages of 18 and 49 who attended eleven community clinics in the san francisco bay area were studied to investigate differences between smokers and nonsmokers. the 332 smokers and 365 nonsmokers were queried about smoking habits, sexual and reproductive history, and recent diet. cervical mucus specimens were cu ... | 1992 | 1302562 |
| [effect of glucose and lactose on the utilization of citrate by lactobacillus casei subsp. rhamnosus atcc 7469]. | the utilization of citrate by lactobacillus casei subsp. rhamnosus atcc 7469 in a complex medium containing glucose, lactose or citrate was investigated, as an approach to the question of the transport of this acid and the possible relationship with the production of flavour compounds (diacetyl and acetoin). this lactobacillus uses citrate as an energy source in the absence of carbohydrates. external ph and growth increases when citrate is added to complex medium. the presence of citrate does no ... | 1992 | 1302866 |
| binding of lactobacillus reuteri to fibronectin immobilized on glass beads. | human fibronectin was immobilized on glass beads. the beads were used to evaluate binding of lactobacillus reuteri to fibronectin. organisms bound to the glass beads were detected using fluorescence microscopy after treatment with acridine orange. this binding was confirmed and quantified with the use of [3h]-labelled organisms. three strains of lactobacillus reuteri, three strains of lactobacillus acidophilus and one strain of lactobacillus fermentum were tested for binding capacity. l. reuteri ... | 1992 | 1303695 |
| [immunomodulating properties of various microbes--representatives of normal intestinal microflora]. | the impact of oral administration of heat-killed bifidobacteria, lactobacteria, enterococci and bacteroides on mouse resistance to experimental salmonellosis and content of immunoglobulin-synthesizing cells in the proper plate of the small intestine was studied. it was shown that the administration of the killed bifidobacteria, lactobacteria and enterococci increased the animal resistance to experimental salmonellosis infection and induced an increase in the content of immunoglobulin-synthesizin ... | 1992 | 1304130 |
| [histological study of the structure of artificial plaque]. | five cariogenic bacteria were used to produce a plaque, called artificial plaque, by sequential culture in vitro. the results showed that the artificial plaque is very similar to the natural one in morphology and structure. artificial plaque may be taken as an excellent model in vitro for the studies on cariogenicity of dental plaque. | 1992 | 1304541 |
| stability and reconstitution of pyruvate oxidase from lactobacillus plantarum: dissection of the stabilizing effects of coenzyme binding and subunit interaction. | pyruvate oxidase from lactobacillus plantarum is a homotetrameric flavoprotein with strong binding sites for fad, tpp, and a divalent cation. treatment with acid ammonium sulfate in the presence of 1.5 m kbr leads to the release of the cofactors, yielding the stable apoenzyme. in the present study, the effects of fad, tpp, and mn2+ on the structural properties of the apoenzyme and the reconstitution of the active holoenzyme from its constituents have been investigated. as shown by circular dichr ... | 1992 | 1304899 |
| characterization of the stabilizing effect of point mutations of pyruvate oxidase from lactobacillus plantarum: protection of the native state by modulating coenzyme binding and subunit interaction. | point mutations in the gene of pyruvate oxidase from lactobacillus plantarum, with proline residue 178 changed to serine, serine 188 to asparagine, and alanine 458 to valine, as well as a combination of the three single point mutations, lead to a significant functional stabilization of the protein. the enzyme is a tetrameric flavoprotein with tightly bound cofactors, fad, tpp, and divalent metal ions. thus, stabilization may be achieved either at the level of tertiary or quaternary interactions, ... | 1992 | 1304900 |
| reversible dissociation and unfolding of the dimeric protein thymidylate synthase. | conditions for in vitro unfolding and refolding of dimeric thymidylate synthase from lactobacillus casei were found. ultraviolet difference and circular dichroism spectra showed that the enzyme was completely unfolded at concentrations of urea over 5.5 m. as measured by restoration of enzyme activity, refolding was accomplished when 0.5 m potassium chloride was included in the refolding mixture. recombination of subunits from catalytically inactive mutant homodimers to form an active hybrid dime ... | 1992 | 1304920 |
| effects of the spermicidal agent nonoxynol-9 on vaginal microbial flora. | the use of nonoxynol-9--containing vaginal contraceptive preparations increases vaginal (and urethral) colonization by escherichia coli. nonoxynol-9 is toxic to various microorganisms, including lactobacillus acidophilus, but has little or no direct effect on e. coli. l. acidophilus, which is present in the vaginas of most normal women, generates h2o2 which, when combined with peroxidase and a halide, was toxic to e. coli. this toxicity was inhibited by nonoxynol-9 due to the selective destructi ... | 1992 | 1309374 |
| hydrogen peroxide production by lactobacillus species: correlation with susceptibility to the spermicidal compound nonoxynol-9. | facultative anaerobic lactobacilli were recovered from the vaginas of 96.8% of 63 nonpregnant, healthy, premenopausal women. the predominant species were lactobacillus jensenii, lactobacillus acidophilus, and lactobacillus casei. of the women, 74.6% had hydrogen peroxide-producing lactobacilli, 22.2% had non-hydrogen peroxide-producing lactobacilli, and 3.2% had no lactobacilli. none of the 68 isolates had catalase activity. some 68.2% of the isolates were inhibited by concentrations of less tha ... | 1992 | 1316413 |
| humoral igg antibodies to oral microbiota in a population at risk for root-surface caries. | mutans streptococci have been strongly implicated in the initiation of dental caries on coronal surfaces. their role in development of root-surface caries is less clear. the etiologic agents of both types of dental caries are likely to elicit systemic immune responses. the objective of the present study, therefore, was to study the association of clinical variables of disease with humoral igg antibodies to nine oral micro-organisms in 314 adult subjects, aged 45-65 years, who were at risk for ro ... | 1992 | 1321176 |
| enhancement of the circulating antibody secreting cell response in human diarrhea by a human lactobacillus strain. | human lactobacillus sp strain gg (lactobacillus gg) administered during acute rotavirus diarrhea has been shown to promote clinical recovery. to elucidate the immune mechanisms behind such a favorable outcome, the elispot (solid phase enzyme-linked immunospot) assay of ig- and specific antibody-secreting cells among circulating lymphocytes was used, giving indirect evidence of the immunologic events in the gut. after rehydration, 39 children with acute rotavirus diarrhea, mean age 16 (sd 6) mo, ... | 1992 | 1324462 |
| isolation and characterization of is1165, an insertion sequence of leuconostoc mesenteroides subsp. cremoris and other lactic acid bacteria. | we have cloned and characterized an insertion sequence from leuconostoc mesenteroides subsp. cremoris strain db1165. this element, designated is1165, is 1553 bp, has imperfect inverted repeat ends, contains an open reading frame of 1236 bp, and is not related to any previously described insertion sequence. the copy number of is1165 varies from 4 to 13 in l. mesenteroides subsp. cremoris strains allowing genetic fingerprinting of strains based on location and number of bands on hybridization. is1 ... | 1992 | 1325060 |
| systematics of the lactobacillus population on rat intestinal mucosa with special reference to lactobacillus reuteri. | the systematics of the lactobacillus population of the intestines of 88 different rats was studied; 80 rats had been fed on fermented oat-meal soup (molin et al. 1992). one-hundred-twenty-two lactobacillus strains from the intestinal mucosa were phenotypically classified together with twenty-eight reference strains of lactobacillus and leuconostoc, using 49 unit characters. data were examined using jaccard coefficient, and unweighted pair group algorithm with arithmetic averages. two major and e ... | 1992 | 1325752 |
| correlation between hydrophobicity and resistance to nonoxynol-9 and vancomycin for urogenital isolates of lactobacilli. | seven clinical isolates of lactobacilli were found to be relatively hydrophobic with a mean water-contact angle of 66 +/- 15 degrees, and to be susceptible to 1% nonoxynol-9 and vancomycin. however, seven other strains were relatively hydrophilic with a mean water-contact angle of 32 +/- 13 degrees, and found to be resistant to 25% nonoxynol-9 and vancomycin. thus, the surface properties of lactobacilli that influence susceptibility to antimicrobial agents may involve surface hydrophobicity. pos ... | 1992 | 1325935 |
| distribution of tnf endogenously induced by various immunopotentiators and lactobacillus casei in mice. | intravenous administration of heat-killed lactobacillus casei yit 9018 (lc) elicited endogenous cytotoxic factor (cf) production in icr mice, peaking in serum after 2 h and declining gradually to basal level at 23 h. the endogenous cf production was significantly enhanced by priming with high molecular-weight lignins and glucans, but not by phenylpropenoid precursors or partially hydrolyzed products of glucans. the extent of stimulation of cf production by these priming agents was positively rel ... | 1992 | 1327255 |
| comparative in-vitro activity of azithromycin, macrolides (erythromycin, clarithromycin and spiramycin) and streptogramin rp 59500 against oral organisms. | the in-vitro activities of azithromycin, clarithromycin, spiramycin and rp 59500 were compared with erythromycin against a wide range of oral organisms which have been implicated in oral infections and/or endocarditis (clindamycin was included for oral streptococci). all compounds tested showed good activity against many of these organisms, although some variation was observed with different species. clarithromycin was the most active of the antibiotics tested against gram-positive anaerobes, in ... | 1992 | 1331019 |