| quantitative studies of the vaginal flora of healthy women and of obstetric and gynaecological patients. | | 1978 | 682174 |
| dihydrofolate reductases in some folate-requiring bacteria with low trimethoprim susceptibility. | dihydrofolate reductases from the folate-requiring strains streptococcus faecalis atcc 8043, lactobacillus casei atcc 7496, and pediococcus cerevisiae atcc 8081, as well as from lactobacillus arabinosus, which is not dependent on exogenous folate, were isolated, and their properties were compared to reductases of escherichia coli b, staphylococcus aureus, and rat liver reductase. an inhibition profile with six different inhibitors revealed significant differences among all enzymes. all lactobaci ... | 1978 | 686702 |
| ribonucleoside triphosphate reductase from lactobacillus leichmannii. | | 1978 | 692388 |
| nucleoside deoxyribosyltransferase from lactobacillus helveticus. | | 1978 | 692395 |
| dental caries induction in experimental animals by clinical strains of streptococcus mutans isolated from japanese children. | oral implantation and the cariogenic activity of clinical strains of streptococcus mutans which had been isolated from japanese children and labeled with streptomycin-resistance were examined in specific pathogen-free sprague-dawley rats. all the seven strains tested were easily implanted and persisted during the experimental period. extensive carious lesions were produced in rats inoculated with clinical strains of s. mutans belonging to serotypes c, d, e, and f, and maintained on caries-induci ... | 1978 | 692465 |
| calorimetric identification of several strains of lactic acid bacteria. | a microcalorimetric method has been developed for the identification of 9 representative strains of lactic acid bacteria used in the dairy industry. a chemically defined medium containing several carbohydrates gave reproducible and characteristic growth patterns. the technique is proposed as a rapid alternative method for the identification of bacteria selected for commercial use. | 1978 | 711958 |
| nalpha-(pteroyltetra (gamma-glutamyl))-lysine as a ligand for the purification of thymidylate synthetase by affinity chromatography. | nalpha-(pteroyltetra (gamma-glutamyl))-lysine sepharose was synthesized and shown to be a stable high capacity affinity matrix capable of bringing about the purification of lactobacillus casei thymidylate synthetase to maximum specific activity from crude extracts in high yield. under conditions optimal for binding of thymidylate synthetase, dihydrofolate reductase was not bound. | 1978 | 714878 |
| [bacteriotherapy of acute radiation sickness]. | | 1978 | 715204 |
| amino acids produced by bifidobacteria and some clostridia. | a lot of 121 strains of bifidobacteria and 9 strains of clostridia were examined for their ability to release free amino acids in the culture broth. the bifidobacteria studied belong to 18 species or "homology group" and the clostridia to 8 species. the growth in a synthetic medium with ammonium salts as sole nitrogen source was also studied. all the clostridia and the majority of the bifidobacteria produce various amino acids. the possible ecological significance of these findings is suggested. | 1978 | 718022 |
| comparative in vitro activity of newer cephalosporins against anaerobic bacteria. | the in vitro susceptibilities of 408 recent clinical isolates of anaerobic bacteria against cefaclor, cephalexin, cephalothin, cefazolin, cefamandole, and cefoxitin were compared by an agar dilution technique. against gram-positive bacteria, especially peptococci, peptostreptococci, and propionibacteria, cephalexin and cefaclor were significantly less active than cephalothin (p < 0.05). cephalexin was also less active than cephalothin against clostridia and lactobacillus (p < 0.05). against gram ... | 1978 | 727759 |
| susceptibility of 40 lactobacilli to six antimicrobial agents with broad gram-positive anaerobic spectra. | the minimal inhibitory concentrations (mics) and minimal bactericidal concentrations (mbcs) of 40 lactobacillus strains were determined against six antibiotics with broad anaerobic spectra. penicillin, ampicillin, clindamycin, and cephalothin were the most active inhibitory agents, with 95 to 100% of the strains inhibited at clinically achievable serum levels. however, despite the inhibitory efficacy of these four agents, only 5 to 22% of the isolates were killed at achievable concentrations. mb ... | 1978 | 727761 |
| substrate specificity of undecaprenyl pyrophosphate synthetase from lactobacillus plantarum. | | 1978 | 728420 |
| [the microflora of sour dough. iv. communication: bacterial composition of sourdough starters genus lactobacillus beijerinck (author's transl)]. | the bacterial composition of three so called pure culture sourdough starters of varying origin was investigated. 245 isolates were obtained all belonging to the genus beijerinck. according to their morphological, physiological and biochemical characteristics they were classified into the subgroups: thermobacterium (l. acidophilus), streptobacterium (l. casei, l. plantarum, l. farciminis, l. alimentarius) and betabacterium (l. brevis, l. brevis var. lindneri, l buchneri, l. fermentum, l. fructi v ... | 1978 | 735416 |
| changes in the caecal flora associated with the onset of laminitis. | caecal fluid samples collected 8 and 24 hours after carbohydrate overload were quantitatively compared to control samples in terms of aerobic and anaerobic bacteria. concomitant increases in lactic acid-producing bacteria and decreases in gram negative bacteria were substantiated during the onset of acute laminitis. progressive decreases in caecal fluid ph were also quantitated. although endotoxin assays of caecal fluid and blood were not done, the caecal flora changes suggest its presence durin ... | 1978 | 738266 |
| bacterial flora in the digestive tract of cattle. ii. development of the flora in the digestive tract in calves. | | 1978 | 740044 |
| bacteremia due to bifidobacterium, eubacterium or lactobacillus; twenty-one cases and review of the literature. | twenty-one cases of bacteremia due to bifidobacterium, eubacterium and lactobacillus are described. transient bacteremic episodes with these organisms may follow trauma to the mouth, intestine, or vagina. the majority of the patients were female and most had an underlying condition that may have predisposed to bacteremia. ten of the patients died despite antibiotic treatment. | 1978 | 749356 |
| [transfer of the fecal microbial flora from holoxenic piglets and adult pigs to axenic piglets and axenic adult mice : effect of the animal host and of the diet on the fecal microbial pattern of these animals (author's transl)]. | the purpose of the present work was to determine whether the axenic mouse inoculated with pig fecal microflora represents a valuable tool for studying the gastro-intestinal flora of holoxenic pigs. the microbial pattern of the feces of the different animals has been described by means of quantitative differential techniques of analysis, the limits and validity of which are discussed. the gnotoxenic mouse associated with the flora of piglet is a better model as the gnotoxenic mouse associated wit ... | 1978 | 754573 |
| folate conjugase activity in fresh vegetables and its effect on the determination of free folate content. | the levels of naturally-occurring folate conjugates in fresh asparagus, broccoli, brussels sprouts, cabbage, cauliflower lettuce, and spinach were determined together with the effect on the hydrolysis of pteroylpolyglutamates during the extraction phase of the lactobacillus casei assay. the levels of conjugate activity vary widely among the vegetables tested, from a low of 0.30 +/- 0.21 in lettuce to a high of 106.2 +/- 6.6 mumoles/hr per 100 mg of fresh weight in spinach. the free folate values ... | 1979 | 760505 |
| radioimmunoassay for pantothenic acid in blood and other tissues. | we describe a radioimmunoassay for pantothenic acid in biological tissues. d-pantothenic acid was conjugated with bovine serum albumin by use of a bromoacetyl derivative of pantothenic acid, and antibody to this antigen was raised by injecting it into the foot pads of rabbits. for the radioimmunoassay, a 100-fold dilution of the resulting antiserum was incubated with radiolabeled pantothentic acid. the antibodies were precipitated and dissolved, and the radioactivity of the solution was measured ... | 1979 | 761345 |
| the amino acid sequence of dihydrofolate reductase from the mouse lymphoma l1210. | the determination of the amino acid sequence of the dihydrofolate reductase (ec 1.5.1.3) from cells of the mouse lymphoma l1210 is described. the protein was cleaved by cyanogen bromide to produce the six fragments cb1 (residues 1 to 14), cb2 (residues 15 to 52), cb3 (residues 53 to 111), cb4 (residues 115 to 125), cb5 (residues 126 to 139), and cb6 (residues 140 to 186). one of the fragments, cb2, contained an internal homoserine derived from a methionine which was not cleaved by cyanogen bromi ... | 1979 | 762074 |
| indigenous microorganisms prevent reduction in cecal size induced by salmonella typhimurium in vaccinated gnotobiotic mice. | germfree cd-1 mice challenged by the oral route with salmonella typhimurium had ceca that were abnormal in appearance and reduced in size compared to those of germfree controls. similarly, germfree mice injected with heat-killed s. typhimurium or gnotobiotes associated with three indigenous microbes (lactobacillus, bacteroides, clostridium), and subsequently challenged with s. typhimurium also had small ceca. by contrast, gnotobiotic mice that had been both injected with the heat-killed s. typhi ... | 1976 | 765282 |
| active site of ribonucleoside diphosphate reductase from escherichia coli. inactivation of the enzyme by 2'-substituted ribonucleoside diphosphates. | ribonucleoside diphosphate reductase is an allosteric enzyme consisting of two nonidentical subunits, proteins b1 and b2. b1 contains dithiols which participate in the oxidation-reduction reactions of electron transport, while b2 contains a free radical essential for activity. ribonucleoside diphosphates are bound to b1 but not to b2. addition of 2'-deoxy-2'-chloro ribonucleoside diphosphates to ribonucleotide reductase irreversibly inactivates b1 without affecting b2. the reaction is specific s ... | 1976 | 767333 |
| synthesis and microbiological activities of beta-(1-chloro-2-naphthyl) alanine and beta-(1-bromo-2-naphthyl) alanine. | beta-(1-chloro-2-naphthyl)alanine and beta-(1-bromo-2-naphthyl) alanine were synthesized by ammonolysis of the corresponding alpha, 1-dihalo-2-naphthalenepropanoic acids derived from 1-nitro-2-naphthylamine by diazotization and condensation with acrylic acid in the presence of cuprous halides. the two analogs as well as the previously reported beta-(2-naphthyl)alanine and beta-(1-naphthyl)alanine were studied as growth inhibitors of escherichia coli 9723, leuconostoc dextranicum 8086, and lactob ... | 1976 | 768475 |
| host-parasite relationships in the alimentary tract of domestic birds. | | 1976 | 769505 |
| alterations in small-bowel microflora in acute intestinal obstruction. | small-bowel content was examined bacteriologically whilst fresh and after storage for 24, 48, and 72 h in a modified stuart's medium. there was little alteration in the viable count of individual intestinal organisms. for accurate quantitative analysis, 0-3 ml of intestinal content was enough. specimens of small-bowel content were obtained by needle aspiration, and a qualitative and quantitative study was made of the microflora of patients with acute intestinal obstruction and of a control group ... | 1976 | 772213 |
| the prevention of recurrent urinary tract infection in women. | | 1976 | 772658 |
| dynamics of mixed cultures of lactobacillus plantarum and propionibacterium shermanii. | the mixed culture of lactobacillus plantarum and propionibacterium shermanii grown anaerobically in glucose minimal medium exhibits features typical of a commensal interaction even though a number of complicating factors, such as a large maintenance requirement of l. plantarum and inhibition of growth of p. shermanii at low ph, are present. a simple mathematical model of the system is presented and is shown to reproduce rather well some of the features of the continuous mixed culture system in b ... | 1976 | 773448 |
| shikimic acid. | | 1976 | 773799 |
| the application of miniaturized methods for the characterization of various organisms isolated from the animal gut. | | 1976 | 773917 |
| the microbial ecology of the upper small bowel. | | 1976 | 775978 |
| influence of certain indigenous gastrointestinal microorganisms on duodenal alkaline phosphatase in mice. | alkaline phosphatase activity was assayed in duodenal homogenates or extracts from adult specific pathogen-free (spf) and germfree mice and gnotobiotic mice monoassociated with a lactobacillus sp., a bacteroides sp., or a coliform strain indigenous to spf mice. activity levels of the enzyme were much higher in the preparations from germfree mice than in those from the spf controls. in the gnotobiotes monoassociated either with a freshly isolated lactobacillus sp. or a bacteroides sp., the levels ... | 1976 | 779647 |
| hot-loop test for the determination of carbon dioxide production from glucose by lactic acid bacteria. | a hot-loop test was found to be more rapid, convenient, and reliable for the detection of carbon dioxide production than were conventional methods such as displacement of agar plugs and precipitation in barium hydroxide. | 1976 | 779651 |
| acyl carrier protein metabolism and regulation of fatty acid biosynthesis by lactobacillus plantarum. | endogenous fatty acid biosynthesis in the bacterium lactobacillus plantarum is greatly decreased upon addition of exogenous fatty acids (henderson, t.a., and mcneil, j.j. (1966) biochem. biophys. res. commun. 25, 662-669). we have demonstrated the presence of five pantothenate-containing compounds in l. plantarum which have been identified by co-chromatography with authentic samples: pantothenate, 4'-phosphopantetheine, 3'-dephosphocoenzyme a, coenzyme a, and acyl carrier protein (acp). the conc ... | 1976 | 780354 |
| [treatment of acute dysentery with new bacterial preparations]. | | 1975 | 781396 |
| investigations on a metabolite from lactobacillus bulgaricus which neutralizes the effect of enterotoxin from escherichia coli pathogenic for pigs. | | 1976 | 783106 |
| initiation of protein synthesis. | | 1976 | 787809 |
| influence of acidosis on rumen function. | | 1976 | 789319 |
| effect of various milk feeds on numbers of escherichia coli and bidifobacterium in the stools of new-born infants. | escherichia coli was found in a similar proportion of stool specimens from infants who were breast-fed and from others fed on three different artificial-milk preparations. when e. coli was present its mean colony count in the stools of breast-fed infants was within the range of the mean counts for infants receiving the artificial -milk feeds. there was no consistent relation between high counts of bifidobacteria (lactobacillus bifidus) and low counts of e. coli. this suggests that measures aimed ... | 1976 | 789762 |
| the ecology and taxonomic status of the lactobacilli. | | 1976 | 791069 |
| iron-binding proteins and other factors in milk responsible for resistance to escherichia coli. | human milk contains large amounts of the iron-binding protein lactoferrin. this is normally unsaturated with iron. it also contains large amounts of iga and small amounts of igg and igm. a combination of lactoferrin and specific antibody has a powerful bacteriostatic effect on escherichia coli. in sucking infants the milk proteins probably reach the small intestine intact. experiments with sucking guinea pigs show that milk suppresses e. coli in the gut and that the unsaturated iron-binding prot ... | 1976 | 791596 |
| alterations in the fecal flora of an individual frequently lacking coliforms. | an individual who usually completely lacked or had very low numbers of coliforms in his stool over a period of 19 months was identified. the subject's levels of clostridia and aerobic lactobacilli were elevated as compared with those of our control group. however, these elevated populations of microorganisms were not related to the number of coliforms present in stool samples. the number of coliforms was inversely related to the number of anaerobic lactobacilli. in addition, the absence of colif ... | 1976 | 792357 |
| [studies of cell arrangement in bacterial colonies by scanning electron microscopy--application of co2 critical-point drying technic]. | | 1976 | 794527 |
| oral flora in edentulous and denture wearers in egypt. | | 1976 | 801704 |
| synthesis and microbiological activities of some monohalogenated analogs of tyrosine. | 2-chlorotyrosine and 2-bromotyrosine, as well as the previously reported 2-fluorotyrosine, were synthesized by hydrolysis of the condensation products from the appropriate benzyl bromide and ethyl acetamidomalonate and were compared with the corresponding 3-halotyrosines as growth inhibitors of escherichia coli 9723, streptococcus faecalis 8043 and lactobacillus plantarum 8014. in contrast to the 2- and 3-fluorotyrosines which were equally effective as growth inhibitors, the 2-chloro- and 2-brom ... | 1975 | 803244 |
| degradation of n-nitrosamines by intestinal bacteria. | a major proportion of bacterial types, common in the gastrointestinal tract of many animals and man, were active in degrading diphenylnitrosamine and dimethylnitrosamine, the former being degraded more rapidly than the latter. at low nitrosamine concentrations (is less than 0.05 micronmol/ml), approximately 55% of added diphenylnitrosamine, 30% of n-nitrosopyrrolidine, and 4% of dimethylnitrosamine were degraded. the route of nitrosamine metabolism by bacteria appears to be different from that p ... | 1975 | 803287 |
| [clinical experience with the preparation hylacombun in gastroenterology (author's transl)]. | | 1975 | 803416 |
| a yeast-like infection of the esophagus caused by lactobacillus acidophilus. | | 1975 | 803465 |
| turnover of the cell wall peptidoglycan of lactobacillus acidophilus. the presence of a fraction immune to turnover. | exponentially growing cultures of lactobacillus acidophilus strain 60am gasser were previously shown to lose about one-third of their cell wall peptidoglycan per generation via turnover (boothby, d., daneo-moore, l., higgins, m. l., coyette, j., and shockman, g. d. (1973) j. biol. chem. 248, 2161-2169). we now show that 20 to 30% of the [3h]lysine initially present in insoluble peptidoglycan fractions was retained after 4 or more generations of continued exponential growth of cultures in the abs ... | 1975 | 803503 |
| susceptibility of anaerobic bacteria to carbenicillin, cefoxitin, and related drugs. | the agar dilution technique was used for determination of the bacteriostatic activity of carbenicillin, penicillin g, cefazolin, cephaloridine, cefoxitin, and cephalothin agaomst a variety of anaerobic bacteria. carbenicillin showed a high level of activity at a concentration of smaller than or equal to 100 mug/ml; only five of 123 strains of bacteroides fragilis, one strain of bifidobacterium eriksonii, and one strain of clostridium bifermentans were resistant to a concentration of larger than ... | 1975 | 804023 |
| lactobacillus casei endocarditis. | | 1975 | 804288 |
| serum pyridoxal in active peptic ulceration. | fasting serum pyridoxal was assayed by an automated microbiological system in 50 patients with endoscopically confirmed active peptic ulceration. thirty patients had gastric ulceration, 14 had duodenal, four had pyloric canal ulceration, and two had both a gastric and a duodenal ulcer. serum pyridoxal was below normal in 28 of the gastric ulcer group and in one of the duodenal ulcer group. no difference in the age, sex, drug, alcohol intake, or diet could be distinguished between those two group ... | 1975 | 804431 |
| distribution of indigenous bacteria in the digestive tract of conventional and gnotobiotic rats. | the localization and population levels of the indigenous bacterial flora of conventional rats were investigated by cultural and histological techniques. lactobacilli predominate in the stomach and upper part of the small intestine and associate with keratinized cells of the nonglandular portion of stomach. mixtures of varying complexity of pure cultures of indigenous bacteria were inoculated into germfree rats. the distribution of these bacteria was examined to investigate the effect of lactobac ... | 1975 | 804450 |
| [administration of bacterial cultures of lactobacillus acidophilus (acidophilic milk) in an endemic focus of dysentery]. | | 1975 | 804691 |
| effects of cadmium on the growth and uptake of cadmium by microorganisms. | six species of microorganisms, escherichia coli, bacillus cereus, lactobacillus acidophilus, staphylococcus aureus, streptococcus faecalis and actinomyces niger, were grown under suitable conditions in appropriate media. cadmium chloride was added to provide 0, 5, 10, 20, 40, and 80 mug of cd per ml. at 40 and 80 mug of cd per ml, e. coli and b. cereus grew well and the other species were repressed. cd uptake patterns differed significantly among the species tested. the significance of these dat ... | 1975 | 804857 |
| studies of folic acid compounds in nature. iv. folic acid compounds in soybeans and cow milk. | folate compounds in soybean and cow's mild were identified by sephadex chromatography and differential microbiological assaymsoybean contained mainly monoglutamates (ca. 52%), some diglutamates (ca. 16%), and polyglutamates (pentaglutamates representing the major portion). 5-cho-h4pteglun(1-6) constituted 65-70% of total folate activity; cow's milk contained monoglutamates (60%) and polyglutamates (ranging from di- to hepta-conjugates). in contrast to soybean, 90-95% of milk folate was in the 5- ... | 1975 | 804977 |
| effect of lactobacilli and carbon dioxide on the growth of microbacterium thermosphactum on fresh beef. | studies with articifially inoculated fresh beef showed that lactobacilli markedlyrestrict the growth of microbacterium thermosphactum when the beef is vacuum-packaged. high concentrations of carbon dioxide (up to 75%) had little effect on the growthof m. thermosphactum under either aerobic or anaerobic conditions. tests were madewith samples of lean beef inoculated with pure cultures of lactobacilli and (or) m. thermosphactum and stored unpackaged in air, in air enriched with carbon dioxide, in ... | 1975 | 804984 |
| lengths of poly-gamma-glutamyl chains in natural folates. | | 1975 | 805391 |
| [electrophoretic characteristics of lactate dehydrogenase isoenzymes of lactobacilli and determination of their activity]. | lactate dehydrogenase isoenzymes of 10 museum strains of lactobacilli were examined electrophoretically in polyacrylamide gel. with respect to the relative electrophoretic motility and enzymic activity certain differences in the isoenzyme spectra of lactate dehydrogenase can give additional evidence for the identification of lactobacillar species. | 1975 | 805421 |
| prevention of folate deficiency by food fortification. ii. absorption of folic acid from fortified staple foods. | the absorption of folic acid from maize, rice and bread fortified with pteroylglutamic acid was compared with that of an aqueous solution of pteroylglutamic acid in the same subjects. feeding fortified maize and rice produced similar increments in folic acid concentration, which were approximately half those observed with the pteroylglutamic acid solution. fortified bread produced a lower increment. pteroylglutamic acid was found to resist destruction by boiling and baking at temperatures and ti ... | 1975 | 805518 |
| analysis of nonlinear response in microbiological assay for folacin. | | 1975 | 805553 |
| tagatose-1, 6-diphosphate activation of lactate dehydrogenase from streptococcus cremoris. | | 1975 | 805590 |
| the metabolic consequences of vitamin b-12/methionine deficiency in rats. | the synthesis of liver pteroylglutamates (folates) from injected [3-h]-pteroylglutamic acid was investigated in vitamin b-12 methionine-deficient rats and pair-fed controls using improved extraction and chromatographic procedures to identify the monoglutamyl derivatives present. livers from deficient animals had significantly increased levels of radioactive 5-methyltetrahydropteroylglutamate detected after conjugase treatment of extracts and significantly decreased levels of radioactive tetrahyd ... | 1975 | 805600 |
| antiplasmodial efficacy of 2,4--diaminopyrimidine0sylfonamide combinations, especially against chloroquine-resistant malaria. | this presentation deals with the historical development of the antifolate pyrimidines and related compounds, first as antimalarial substances and later as potent antibacterial agents. it describes the first quantitation of the combined action, through sequential blockade, of the substances with sulfonamides, and outlines the usefulness of the combinations in the therapy of normally sensitive and multiresistant strains of plasmodium falciparum. | 1975 | 805650 |
| a numerical taxonomic study of coryneform and related bacteria. | two hundred and thirty-three strains of coryneform bacteria, including representatives of the genera arthrobacter, brevibacterium, cellulomonas, corynebacterium, erysipelothrix, jensenia, kurthia, listeria, microbacterium, mycobacterium, nocardia and propionibacterium and other related bacteria, were studied using 173 morphological, physiological and biochemical tests. the bacteria were grown on a soil extract medium which allowed growth of all the strains, and all were incubated at 30 degrees c ... | 1975 | 805825 |
| prevalence and antibiotic susceptibility of organisms isolated from acute soft-tissue abscesses secondary to dental caries. | | 1975 | 806046 |
| transfer of folate to the fetus. | the accumulation of tritium-labelled folate in the product of conception was examined in eight women undergoing hysterotomy for termination of pregnancy. folic acid containing a small quantity of tracer material (50n ci/mug.) was injected intravenously 20 minutes before operation in 4 patients but 24 hours beforehand in the remainder. after operation, maternal blood, placenta, fetal blood, and fetal liver were examined for radioactivity as was the daily urine output during the patient's postoper ... | 1975 | 806294 |
| folic acid absorption in pregnancy. | folic acid absorption was studied in six non-pregnant women, and nine pregnant women undergoing therapeutic termination of pregnancy between 10 to 26 weeks, using 2 mg. of folic acid labelled with 10 muci of tritiated folic acid. absorption varied between 65 and 95 per cent, with a mean absorption of 80 per cent, in both pregnant and non-pregnant subjects. similarly, there was no difference of retention between the groups. the results indicate that there is no change in the absorption of folic a ... | 1975 | 806295 |
| study of thymidylate synthetase-function by laser raman spectroscopy. | the laser-raman spectra of thymidylate synthetase have been obtained with 488 nm excitation from an argon ion laser. raman bands observed in the range 600-800 cm-minus-1 have been assigned to functional groups of constituent amino acids. the band positions and intensities in the amide i (1600-1700 cm-minus-1) and amide iii (1200-1300 cm-minus-1) regions, suggest that the enzyme is a mixture of alpha-helical and unordered conformations. low levels of beta-structure cannot be excluded. the spectra ... | 1975 | 806300 |
| some observations on the caecal microflora of the chick during the first two weeks of life. | 1. changes in the caecal flora of chicks aged from approximately 3 h to 14 d were studied. 2. counts obtained by means of a conventional anaerobic plating method and a method for the stricter anaerobes remained comparable throughout but occasionally it was found that strains of anaerobic bacteria isolated from the predominant flora of 4- to 14-d-old birds could not be grown when tested by the conventional method. 3. during the 14-d period approximately 100% of the organisms isolated on anaerobic ... | 1975 | 806330 |
| behavior of microflora in the rat stomach: adhesion of lactobacilli to the keratinized epithelial cells of the rat stomach in vitro. | certain indigenous bacteria isolated from rats and chickens were found to adhere to keratinized cells obtained from host stomachs and maintained in vitro. only lactobacilli and staphylococci isolated from rats attached to the keratinized epithelial cells of the rat stomach, suggesting that they are dominant bacteria in the microflora of the stomachs of those animals. indigenous lactobacilli heated at 60 c for 1 h or treated with the detergents sodium dodecyl sulfate, tween 80, and triton x-100 l ... | 1975 | 806531 |
| effect of sodium phytate on the chemical and microbial composition of dental plaque in the monkey (macaca fascicularis) | the incorporation of 1 or 3% sodium phytate in confectioners sugar produced minimal changes in the physical,chemical, and microbial composition of dental plaque in tube-fed monkeys during a two-week period. only a reduction in yeasts and lactobacilli could be ascribed to the presence of phytate. other changes were attributable to the transition from conventional feeding to tube-feeding, irrespective of the presence of absence of phytate. | 1975 | 806617 |
| in vitro determination of the antimicrobial properties of two cyanoacrylate preparations. | isobutyl and trifluoro cyanoacrylates showed varying degrees of inhibition for lactobacillus casei and staphylococcus aureus when tested by the spread plate technique. candida albicans and pseudomonas aeruginosa were resistant. the results tend to support the view that inhibition of growth was due to the vapor effect and not the diffusibility of the cyanoacrylates. | 1975 | 806619 |
| lactobacillus sp. isolates from mouse livers: saprophyte or potential pathogen? | over a 3-year sampling period lactobacillus sp. has been isolated from the livers of barrier-maintained and conventional mice. serology has failed to detect presence of mouse hepatitis virus antibodies in any of these mice. histopathological examination revealed only mild fatty change and small monocytic infiltrates. | 1975 | 806745 |
| the effect of age on the blood and liver folate concentrations of laboratory animals. | | 1975 | 806913 |
| experimental production of elevated serum folate in dogs with intestinal blind loops. ii. nature of bacterially produced folate coenzymes in blind loop fluid. | jejunal and ileal blind loops were constructed in separate groups of dogs and the folate coenzymes present in these loops were investigated. although only those dogs with high blind loops had appreciably elevated folate levels active for lactobacillus casei but not streptococcus faecalis, every blind loop appeared to contain four folate compounds--5-formyltetrahydrofolic acid, 5-methyltetrahydrofolic acid, tetrahydrofolic acid and a compound tentatively identified as 5-formyltetrahydrofolic trig ... | 1975 | 807096 |
| enzymatic demodification of transfer rna species containing n-6-(delta-2-isopentenyl)adenosine. | | 1975 | 807214 |
| the effect of substrate analogs on the circular dichroic spectra of thymidylate synthetase from lactobacillus casei. | circular dichroism studies from 290 to 400 nm with the thymidylate synthetase from lactobacillus casei revealed characteristic cotton effects in the presence of various folate analogs plus 5-fluoro-2'-deoxyuridylate. omission of either substrate analog prevented the appearance of the cotton effects. when 5-fluoro-2'-deoxyuridylate and (+/-)-5,10-methylenetetrahydrofolate are mixed with the synthetase, a ternary complex results which yields distinctive minor negative ellipicity bands at 285 and 3 ... | 1975 | 807243 |
| interaction of deoxyuridylate with thymidylate synthetase. | the reaction of deoxyuridylate with lactobacillus casei thymidylate synthetase (methylenetetrahydrofolate: deoxyuridylate c-methyltransferase) in the absence of folate or thiols can be followed by changes in the circular dichroic spectra at 267 nm. these changes at the maximum absorption wavelength of the pyrimidine suggest that on binding the interaction of the pyrimidine ring and the deoxyribose moiety is altered. the binding curve follows the form expected for single site binding. there was n ... | 1975 | 807570 |
| folate concentration and pattern in bovine milk. | | 1975 | 807607 |
| synthesis of a fluorescent derivative of amethopterin,. | fluorescein isothiocyanate was treated with excess diaminopentane and the remaining unsubstituted amino group of the product was condensed, via a carbodiimide-promoted reaction, with a carboxyl group of amethopterin. the final product, a fluorescent derivative of amethopterin, was isolated by chromatography on ae-cellulose and preparative electrophoresis on polyacrylamide. it was shown to be homogeneous by analytical polyacrylamide electrophoresis and thin-layer chromatography. proof of structur ... | 1975 | 807734 |
| competitive effects of intestinal microflora on vibrio cholerae in gnotobiotic mice. | the coexistence of vibrio cholerae and several intestinal bacteria was determined in gnotobiotic mice. the bacteria tested included a bacteroides sp, clostridium difficile, clostridium perfringens type a, 2 separate isolates of escherichia coli, 2 different lactobacilli, 2 separate isolates of proteus mirabilis, pseudomonas maltophilia, and streptococcus faecalis. each species of bacteria became established and was recovered repeatedly from the stools during the studies. no single strain or spec ... | 1975 | 807775 |
| [fluorescence bacterioscopy, a direct method for bacteriological food analysis]. | the present bacteriological method which is intended for the simultaneous direct detection of live and dead germs in foods, offers certain difficulties in differentiating between bacterial cells and food consituents of similar forms. furthermore, gram-negative germs are not disclosed. to overcome these difficulties, the authors performed comparative model experiments with gram's stain and the fluorescence method, including pure cultures and foods of animal and vegetable origin. among the fluoroc ... | 1975 | 807849 |
| in vitro study of the influence of virginiamycin and spiramycin on the composition and biochemical activities of the gastrointestinal flora of piglets. ii. influence on the biochemical activities of the microflora. | chemical analysis was carried out on 54 in vitro fermentations to study the influence of nutritional doses of virginiamycin and spiramycin on the metabolic activity of the gastro-intestinal flora of 18 in vivo treated piglets. changes in the decomposition of glucose and the production of nh3, volatile acids and lactic acid are recorded in function of time. these results are in good accordance with changes noted in the composition of the bacterial flora, such as a marked decrease in the productio ... | 1975 | 808048 |
| eimeria brunetti in epithelial cells of the bursa of fabricius in gnotobiotic chickens. | germ-free and conventional chickens were infected with lactobacillus acidophilus and/or eimeria brunetti. germ-free chickens acquiring single infections of e. brunetti were found to have lifecycle stages of the coccidial organism in the bursa of fabricius. | 1975 | 808206 |
| synthesis and biological activity of 10-thia-10-deaza analogs of folic acid, pteroic acid, and related compounds. | the 10-thia analogs of pteroic acid, folic acid, their esters, and their 4-amino analogs were synthesized through a reaction sequence involving, as a key step, the condensation of 2-amino-3-cyano-5-chloromethylpyrazine with appropriately substituted thiols. the abilities of the products to inhibit the growth of methotrexate (mtx)-sensitive and mtx-resistant microorganisms were investigated as were their abilities to inhibit dihydrofolic acid reductase and thymidylic acid synthetase. several comp ... | 1975 | 808608 |
| effect of hyperbaric helium on vitamin uptake and utilization by micro-organisms. | growth responses under hyperbaric helium of various procaryotic micro-organisms to graded levels of six water-soluble vitamins were measured. vitamins included: thiamin, riboflavin, niacin, pantothenic acid, biotin, and folic acid (folacin). growth response of each organism under hyperbaric helium was compared with that under 80% he-20% o2 and other he-o2 gas mixtures at atmospheric pressure, and with response in air at atmospheric pressure. for five of the six vitamins, no differences in respon ... | 1975 | 809032 |
| [effect of the combination of 5-methylquinolin-8-ol, 7-bromo-5-methylquinolin-8-ol and 5-methylquinolin-8-ol-dodecylsulfate on the intestinal flora of healthy subjects]. | the influence of a combination of 5-methyl-quinolin-8-ol, 7-bromo-5-methyl-quinolin-8-ol with 5-methyl-quinolin-8-ol-dodecylsulfate on the composition of intestinal bacteria of healthy adults has been examined. the composition of normal anaerobic and aerobic intestinal bacteria practically showed no change. | 1975 | 809045 |
| ttna isopentenyltransferase from lactobacillus acidophilus atcc 4963. | | 1975 | 809333 |
| transmission of lactobacillus jensenii and lactobacillus acidophilus from mother to child at time of delivery. | the presence of lactobacillus jensenii and lactobacillus acidophilus has been studied in specimens from the rectum and vagina of the mother, from the mouth of the infant at the time of delivery, and from the mouth and rectum of infants six days of age. l. jensenii could be differentiated from other species of lactobacilli by the following combination of characteristics: production of only d-lactate, hydrolysis of arginine, and fermentation of cellobiose, galactose, and ribose, but not of lactose ... | 1975 | 809467 |
| presence of fine spirals (spirosomes) in lactobacillus fermenti and lactobacillus casei. | | 1975 | 809611 |
| radioassay of serum folate with use of pig plasma folate binders. | pig plasma has been used as a source of specific folate binders for the development of a rapid radioassay to measure the concentration of folate in serum. the assay uses 3h-pteroylglutamic acid as tracer and n-5-methyltetrahydrofolic acid for the construction of the standard curve. the assay is run in a two-step incubation system of 15 min each at room temperature. comparison between the results obtained with this method and a microbiological one indicated that for sera with relatively high fola ... | 1975 | 809969 |
| [bacteriocinogeny in lactobacilli of the subgenus streptobacterium]. | bacteriocinogenic properties of 145 strains of l. casei and 23 strains of l. plantarum were studied by the method of delayed antagonism. the capacity for producing bacteriocins was observed in 2 per cent of l. casei and 26 per cent of l. plantarum. the bacteriocins of l. casei and l. plantarum were preliminarily divided into 3 and 2 types respectively by their activity against lactobacilli of different species and the character of the growth inhibition zones of the indicator strain. | 1975 | 810075 |
| competitive protein binding assay for methotrexate. | a competitive protein binding assay has been developed for methotrexate based on the tight binding of this drug to lactobacillus casei dihydrofolate reductase (= tetrahydrofolate dehydrogenase; 5,6,7,8-tetrahydrofolate: nadp+ oxidoreductase; ec 1.5.1.3). free drug may be separated from that bound to reductase by adsorption with dextran--albumin coated charcoal. scatchard plot analysis of the enzyme--drug interaction confirmed the presence of a single homogeneous class of binding sites with an as ... | 1975 | 810804 |
| [influence of some factors on the quality of bulgarian sour milk]. | the results obtained with three feeding experiments revealed that combined forages containing biovit-40 are effective in that the antibiotic is not excreted with cow's milk and it does not inhibit the development of lactic acid fermentation. the season of the year associated with the passage of a given type of feed on to another type was found to influence the course of the lactic acid fermentation as follows: --in feeding green mass in the summer period there is a stimulation of the development ... | 1975 | 810941 |
| detection of lactobacillus acidophilus in feces of humans, pigs, and chickens. | lactobacilli in fecal material from humans, pigs, and chickens were enumerated on lactobacillus selective agar (lbs). in all samples, higher numbers of lac-tobacilli were detected when plates were incubated in a system flushed with co2 rather than in air. much higher numbers of bacteria from human feces were detected when the lbs agar plates were incubated anaerobically in a hydrogen-carbon dioxide atmosphere (gaspak) than when incubated in co2. the bacteria from human feces isolated on lbs agar ... | 1975 | 811162 |
| enterotoxigenic intestinal bacteria in tropical sprue. iv. effect of linoleic acid on growth interrelationships of lactobacillus acidophilus and klebsiella pneumoniae. | the factors responsible for colonization of the small intestine by enterotoxigenic coliform bacteria in puerto ricans with tropical sprue are unknown, but epidemiological observations have suggested that they may be related to an increased dietary intake of long-chain unsaturated fatty acids, particularly linoleic acid, which is known to exert an inhibitory effect on the growth of gram-positive organisms that normally comprise the flora of the small intestine. we have examined, by using a glucos ... | 1975 | 811564 |
| amino acid analogs. iii: new syntheses of monomethyl- and monophenylglutamic acids. | glutamic acid analogs containing 3- and 4-methyl and 2-, 3-, and 4-phenyl substituents were prepared. the 3- and 4-methyl- and 3- and 4-phenylglutamic acids did not inhibit plasmodium berghei and were nontoxic to the host (mice) at 640 mg/kg. the five analogs in addition to 2-methlglutamic acid were inactive against lactobacillus casei at 1000 mug/ml in a defined medium: against escherichia coli, only 2-methylglutamic acid caused 27% inhibition at 10,000 mug/ml. all six analogs failed to inhibit ... | 1975 | 811786 |
| [serological characteristics of lactobacillus casei var. rhamnosus]. | | 1975 | 811828 |
| [immunoglobulins a in the vagina. assay and study of their specificity]. | the authors have titrated, by the laurell technique, immunoglobulin a in vaginal washings ; the quantity of iga found is higher for women having a pathogenic vaginal flora. using immunofluorescence technique, and peroxydase-marked antibodies, the authors found specific iga directed against lactobacillus, n. gonorrhoeae and candida albicans. | 1975 | 812042 |
| ecological studies of the microbial flora of the female lower genital tract. | | 1975 | 812101 |