Publications

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growth of staphylococcus aureus, experimentally inoculated in damietta cheese. 1975870
the interaction of magnesium ions with teichoic acid.the binding of mg2+ to the wall teichoic acid of lactobacillus buchneri n.c.i.b. 8007 was measured by equilibrium dialysis at controlled ionic concentration and ph. in an aqueous solution containing 10mm-nacl at ph 5.0 one mg2+ ion was bound for every two phosphate groups of the teichoic acid, with an apparent association constant, kassoc. = 2.7 x 10(3) m-1. on lowering the ph below the pka of the phosphate groups the amount of bound mg2+ decreased concomitantly with decreasing ionization of the ...1975994
inhibition of bacterial wall lysins by lipoteichoic acids and related compounds. 19751027
altered nutritional requirements associated with mutations affecting the structures of ribonucleic acid polymerase in lactobacillus casei.rifampin-resistant mutants were isolated from lactobacillus casei s1 and examined for possible simultaneous alteration in nutritional properties. among the 36 mutants obtained either spontaneously or after mutagenesis with 2-aminopurine, 22 were found to be altered with respect to the specific growth requirements. the majority (20 of 22) of the latter mutants were shown to require l-glutamine in addition to the nutrients required by the parental strain for maximal growth, whereas the remaining m ...19761379
radioassay for serum and red cell folate.a simple, reliable assay for serum and red cell folate is described. it uses plain untreated liquid or powdered milk, requiring no special handling or purification, as binder. such milk makes it possible to ignore endogenous serum folate binder, since crude (but not purified) milk contains a factor which releases folate from serum binder. it simplifies counting radioactivity by employing a gamma emitting isotope of pteroylglutamic acid (pga), namely the 125i-tyramide of pga. like the 3h-pga assa ...19761452
b-complex vitamins in cultured and acidified yogurt.studies were to determine the effect of various factors upon b-vitamin content of cultured yogurt and to compare the b-vitamin contents of cultured and direct acidified yogurt. incubation of yogurt culture at 42 c for 3 h yielded maximum vitamin synthesis concurrent with optimal flavor and texture qualities. a method was standardized for the manufacture of direct acidified yogurt involving the use of stabilac acidulant and nonfat dry milk, carboxymethyl cellulose, gelatin, and starite. acidified ...19762629
microorganisms and characteristics of laban.laban had a titratable acidity of about 1.0%, a ph of 4.25, an ethanol content of 1.25%, and contained 4.2 mug acetaldehyde and 34 mug acetoin/ml. there was no diacetyl. five microorganisms, classified as streptococcus thermophilus, lactobacillus acidophilus, leuconostoc lactis, kluyveromyces fragilis, and saccharomyces cerevisiae, were responsible for the fermentation. streptococcus thermophilus and l. acidophilus were responsible for acid production with s. thermophilus producing acid more rap ...19762630
citric acid metabolism in hetero- and homofermentative lactic acid bacteria.the effect of citrate on production of diacetyl and acetoin by four strains each of heterofermentative and homofermentative lactic acid bacteria capable of utilizing citrate was studied. acetoin was quantitatively the more important compound. the heterofermentative bacteria produced no acetoin or diacetyl in the absence of citrate, and two strains produced traces of acetoin in its presence. citrate stimulated the growth rate of the heterofermentative lactobacilli. acidification of all heteroferm ...19765054
adherence of bacterial to vaginal epithelial cells.vaginal epithelial cells from healthy women were washed and incubated in tissue culture medium with freshly isolated bacteria of the indigenous vaginal flora and with bacteria of species that have been discussed in conjunction with genital infections. after incubation and washing, the number of bacteria that adhered per cell was determined. the influence on the attachment rate of such factors as variations in the washing procedure, bacterial density, and incubation time was assessed. lactobacill ...19765372
the toxicity of sulphur dioxide towards certain lactic acid bacteria from fermented apple juice. 19765394
investigations on the function of the rat forestomach.the functions of the rat forestomach and upper digestive tract were studied. the ph values, alpha-amylase activity, quantitative estimates of microorganisms, and emptying rates were higher in the forestomach than in the glandular stomach. rats with surgically removed forestomachs lived without complications for more than 1 yr. their alimentary hyperglycemia was higher and shorter than in controls. the significance of rich microflora present in the conventional forestomach is not known, although ...19765626
microbial interactions in foods: meats, poultry and dairy products. 19765780
l-glutamate-glyoxylate aminotransferase in lactobacillus plantarum.glutamate-glyoxylate aminotransferase which mediates the reaction of glyoxylic acid with glutamic acid to yield glycine and alpha-oxoglutaric acid has been isolated and purified 84-fold from extracts of lactobacillus plantarum. purified enzyme requires the addition of pyridoxal phosphate and magnesium ions for its activity. the molecular weight of the enzyme estimated by sepharose 4b gel filtration amounts to 37.000. micaelis constants for glyoxylate and glutamate are corresponding to 6.25 x 10( ...19755859
intestinal ph and propulsion: an explanation of diarrhoea in lactase deficiency and laxation by lactulose.subjects deficient in lactase may experience bloating, cramps and diarrhoea after ingesting milk, due to the unhydrolysed and poorly-absorbed lactose. the diarrhoea may result from an osmotic effect of the lactose itself or its poorly-absorbed acidic products of fermentation (weijers, van de kamer & others, 1961; christopher & bayless, 1971), possibly together with an alteration of sodium and water absorption due to the lowered colonic ph (rousseau & sladen, 1971). laxation by lactulose (1-4-bet ...19766691
d(--)lactic acid formation and d(--)lactate dehydrogenase in octopus spermatozoa. 19766965
lactobacillus plantarum undecaprenyl pyrophosphate synthetase: purification and reaction requirements. 19768010
production of cell-bound proteinase by lactobacillus bulgaricus and its location in the bacterial cell. 19768417
significance of starter cultures for raw sausage aging in view of food and nutrition hygiene.the authors studied the effects of liquid starter cultures on the survival of pathogenic germs. it was found that the foreign bacteria tested differed in growth limitation which is obviously dependent on acidity and the amount of lactic acid produced by fermentation. since the pathogenic bacteria differ in the ability to survive, it is imperative to observe strict hygienic measures in preparing starter cultures and to use absolutely sterile monocultures for raw sausage ageing.19768718
histidine decarboxylase of lactobacillus 30a: function and reactivity of sulfhydryl groups.two classes of sulfhydryl groups in histidine decarboxylase from lactobacillus 30 a can be differentiated by their reaction with 5,5'-dithiobis(2-nitrobenzoic acid) (dtnb). five cysteinyl residues (class i) of the native enzyme are titrated by dtnb as the ph of the reaction medium is increased from 6.5 to 7.5; the ph-rate profile for their reaction is described by a pka of 9.2. an additional five thiol groups (class ii) are titrated only when denaturing agents are added above neutral ph. histidi ...19769132
microbiology of the female genital tract during pregnancy and parturition. 197610196
microbiological assays for dental caries and periodontal disease susceptibility. 197610540
large-scale purification and characterization of dihydrofolate reductase from a methotrexate-resistant strain of lactobacillus casei.dihydrofolate reductase has been purified from a methotrexate-resistant strain of lactobacillus casei ncb 6375. by careful attention to growth conditions, up to 2.5 g of enzyme is obtained from a 400 litre culture. the purification procedure, involving poly-ethyleneimine treatment, deae-cellulose chromatography and affinity chromatography on methotrexate-aminohexyl-sepharose, operates on the gram scale, with overall yields of 50-60%. elution of the affinity column by reverse (upward) flow was us ...197610886
the role of microflora in development of graft-versus-host disease. 197611583
total folate binding capacity of normal human plasma, and variations in uremia, cirrhosis, and pregnancy.the current study presents evidence that all human serum contains a class of high-affinity folate binders (ka=2.8 x10(10 liters/mole), which migrate as a single peak on gel filtration. failure of previous studies to detect this characteristic in all but a minority of subjects is attributable to its variable, often total, saturation. direct measurement of the total folate binding capacity (tfbc) has been made possible by dissociation of endogenous folate-binder complexes at acid ph, removal of fr ...197611860
bacterial fermentation of cheese whey for production of a ruminant feed supplement rich in curde protein.a simple and efficient process for the production of a ruminant feed supplement, rich in crude protein (defined as total n x 6.25), by bacterial fermentation of cheese whey has been developed. the lactose in unpasteurized whey is fermented to lactate acid by lactobacillus bulgaricus at a temperature of 43 degrees c and ph 5.5. the lactic acid produced is continually neutralized with ammonia to form ammonium lactate. the fermented product is concentrated by evaporation to a solids content of abou ...197612720
biological properties of selenobiotin. 197612758
hydrolysis of lactose by immobilized microorganisms.cells of lactobacillus bulgaricus, escherichia coli, and kluyveromyces (saccharomyces) lactis immobilized in polyacrylamide gel beads retained 27 to 61% of the beta-galactosidase activity of intact cells. optimum temperature and ph and thermostability of these microbial beta-galactosidases were negligibly affected by the immobilization. km values of beta-galactosidase in immobilized cells of l. bulgaricus, e. coli, and k. lactis toward lactose were 4.2, 5.4, and 30 mm, respectively. neither inhi ...197713709
deconjugation of bile acids by intestinal lactobacilli.lactobacillus species normally found in the intestinal tract of humans varied in the ability to deconjugate bile acids, whereas laboratory strains of lactobacillus acidophilus deconjugated both glycocholate and taurocholate. all isolates of l. acidophilus from human feces deconjugated taurocholate, whereas only one of six deconjugated glycocholate. none of 13 isolates identified as l. casei deconjugated taurocholate, whereas 9 deconjugated glycocholate. the deconjugating system of l. acidophilus ...197713710
transport and metabolism of vitamin b6 in lactic acid bacteria.streptococcus faecalis 8043 concentrates extracellular 3hpyridoxal or 3hpyridoxamine primarily as the corresponding 5'-phosphates. accumulation of pyridoxamine requires an exogenous energy source and is inhibited by glycolysis inhibitors. a membrane potential is not required for transport of pyridoxamine, and an artificially generated potential does not drive uptake in this organism. based on this and other evidence, it is concluded that s. faecalis accumulates pyridoxamine by facilitated diffus ...197714135
microbial and salivary conditions in 9- to 12-year-old children.the prevalence of s. mutans and lactobacilli, the salivary secretion rate, and salivary ph and buffer capacity were examined in 655 children 9-12 years old. some of these factors were correlated with the frequency of caries. positive correlations were observed between for example s. mutans and lactobacilli, between s. mutans and incipient smooth surface caries, and between lactobacilli and open carious lesion. a high proportion of the children showed large numbers of s. mutans and lactobacilli.197714393
comparative studies of lactic acid dehydrogenases in lactic acid bacteria. i. purification and kinetics of the allosteric l-lactic acid dehydrogenase from lactobacillus casei ssp. casei and lactobacillus curvatus.the stability, ph-dependence and kinetic properties of the mn2+ and fdp-activated nad-dependent lactic acid dehydrogenases from lactobacillus casei ssp. casei (atcc 393) and l. curvatus (dsm 20010) were studied after the enzymes were purified to homogeneity by affinity chromatography. both enzymes are virtually unidirectional, catalysing efficiency only the reduction of pyruvate. they are similar with respect to the effector requirement and ph-optimum. they differ, however, in their electrophore ...197714601
study on l-malic acid catabolism by "lactobacillus casei" cells immobilized into polyacrylamide gel lattice (author's transl).study on l-malic acid catabolism by lactobacillus casei cells immobilized into polyacrylamide gel lattice has shown that the ph profil of malic acid decarboxylase activity does not differ significantly from that of free cells. the apparent enzymatic constant value as determined by the warburg manometric method is 5 x 10(-3) m for free cells and 1.25 x 10(-2) m for immobilized cells. malic acid decarboxylase activity can be preserved in immobilized cells over a 12 month period when the reaction v ...197615492
allosteric and non-allosteric phosphofructokinases from lactobacilli. purification and properties of phosphofructokinases from l. plantarum and l. acidophilus.phosphofructokinase (atp : d-fructose-6-phosphate 1 phosphotransferase, ec 2.7.1.11) from two different lactobacilli, lactobacillus plantarum and lactobacillus acidophilus were isolated and purified. both enzymes have a molecular weight of 154 000 and consist of four subunits of identical size. antisera from sheep immunized against the purified phosphofructokinase from l. plantarum showed immunologic cross reaction with the enzyme from l. acidophilus. in spite of the close molecular relationship ...197715608
ph metric method for the determination of nicotinic acid in plasma.the acidimetric method for the determination of nicotinic acid (na) using lactobacillus plantarum atcc 8014 (lactobacillus arabinosus 17-5) has been simplified and thus made less time consuming, and the sensitivity has been increased fivefold by replacement of the titration by a ph determination. as the regression of the decrease in ph on the amount of na was found linear within a range of 1 to 4 ng of na per ml, the calculations were performed according to the slope-ratio principle. the na conc ...197716033
1h nuclear magnetic resonance studies of lactobacillus casei dihydrofolate reductase: effects of substrate and inhibitor binding on the histidine residues. 197716268
1h nuclear magnetic resonance studies of the tyrosine residues of selectively deuterated lactobacillus casei dihydrofolate reductase. 197716269
lycasin as a sugar substitute. 197716739
purification and properties of a membrane-associated, folate-binding protein from lactobacillus casei.a folate-binding protein has been solubilized from lactobacillus casei by treatment of membrane preparations with triton x-100 in the presence of 3hfolate. the protein-folate complex was purified 100-fold and recovered in a 22% yield by adsorption and elution from microgranular silica (quso g-32), followed by passage through sephadex g-150. when subjected to sodium dodecyl sulfate/polyacrylamide gel electrophoresis, the purified preparations showed only a single, protein-staining band whose mole ...197716917
in vitro inhibition of growth of neisseria gonorrhoeae by genital microorganisms.the ability of microorganisms present in titers of over 10(5)/ml in the vaginal or cervical secretions to inhibit growth of n. gonorrhoeae in vitro was tested. study of a strain of n. gonorrhoeae against 77 microorganisms demonstrated that most strans of s. epidermidis, s. aureus and the one "gaffkya anaerobia" interfered with the growth of the n. gonorrhoeae. a minority of strains of s. viridans, neisseria, candida and bifidobacterium demonstrated interference. no strains of enterococcus, dipth ...197717163
effect of 30 months in a locked environment on the microbial flora of dogs. 197718131
reduction of oxidised folates by dihydrofolate reductase from methotrexate-resistant lactobacillus casei.the use of alternative substrates by dihydrofolate reductase (5,6,7,8-tetrahydrofolate: nadp+ oxidoreductase, ec 1.5.1.3) was investigated as a possible mechanism for the resistance of lactobacillus casei to the cytotoxic drug methotrexate. the reduction of folic acid and 10-formylfolic acid by homogeneous enzyme was compared to that of the normal substrate, dihydrofolic acid. the three substrates have different ph optima and km values. in addition, it was found that the reduction of 10-formylfo ...197718181
transport of pantothenic acid in lactobacillus plantarum. 197718275
19 fnuclear magnetic resonance studies of ligand binding to 3-fluorotyrosine-and 6-fluorotryptophan-containing dihydrofolate reductase from lactobacillus casei. 197719051
31p nmr studies of the binding of adenosine-2'-phosphate to lactobacillus casei dihydrofolate reductase. 197719299
dihydrofolate reductase from amethopterin-resistant lactobacillus casei. effects of ph, salts, temperature, and source of nadph on enzyme activity and substrate specificity studies. 197720050
the importance of lactobacilli in maintaining normal microbial balance in the crop.1. the effect of lactobacilli on escherichia coli has been examined in vitro and in chicken crop in vivo. 2. inhibition of e. coli was dependent on the presence of sufficient numbers of lactobacilli. 3. in a standard test some lactobacilli were bacteriostatic and one was bactericidal. the bacteriostasis was due to the low ph produced by these strains but bactericidal activity could not be accounted for by ph alone. 4. in gnotobiotic animals the bactericidal strain was no more inhibitory for e. c ...197720197
the charge state of substrates and inhibitors when bound to lactobacillus casei dihydrofolate reductase proceedings. 197720381
acid production from lycasin, maltitol, sorbitol and xylitol by oral streptococci and lactobacilli.the acid production from maltitol was compared with the acid production from hydrogenated starch hydrolysate (lycasin), sorbitol and xylitol by a number of oral strains and reference strains of streptococcus mutans, s. sanguis, s. salivarius, s. mitior, s. milleri, s. faecalis, s. faecium, s. avium, lactobacillus casei and l. salivarius. the polyols were added to a final concentration of 1.0% to two different basal media. incubation was performed at 37 degrees c for 7 days after which the ph was ...197721508
international aspects of oral microbiology.it appears that the bacteria of the mouth are remarkably similar throughout the world. there are differences, but they are mostly of a quantitative rather than a qualitative nature. although much is yet to be learned on the regulation of the oral ecosystems, these quantitative fluctuations to a large extent seem to be induced by different dietary patterns: the food exerting a strong selective pressure on the oral microbiota.197721852
changes in the microflora and physiology of the anterior intestinal tract of pigs weaned at 2 days, with special reference to the pathogenesis of diarrhea.the gastrointestinal microflora and gastric physiology of piglets weaned at 2 days was compared with that of piglets allowed to continue sucking the sow. although there was a significantly higher count of escherichia coli in the stomach, duodenum, and jejunum of the early-weaned compared with sow-reared pigs, these differences were not detectable in samples from the ileum. there were no quantitative differences in lactobacilli and in streptococci between the two treatments. lactobacillus ferment ...197722488
binding of lectins to streptococcus mutans cells and type-specific polysaccharides, and effect on adherence.the lectin concanavalin a (con a) agglutinated the cells of 13 of 15 strains of the seven serotypes of streptococcus mutans in an 18-h incubation period. strains of types a, d, f, and g agglutinated within 2 h. strains of a, d, and f were also agglutinated in 2 h by the castor bean lectin rca. s. sanguis, s. salivarius, s. bovis, actinomyces viscosus, a. naeslundii, and lactobacillus plantarum were agglutinated within 2 h. the s. mutans type f polysaccharide was precipitated by con a. the a, b, ...197722491
kinetics of sulfhydryl group modification of thymidylate synthetase: a proposal for activation of catalytic cysteinly residues. 197823783
microbiological measurement of folic coenzymes. ii. effect of the ph of ascorbic acid. 197723813
lactulose: a drug in search of a modus operandi. 197824572
acid potential of streptococci and lactobacilli in the initiation of dental caries. 197525307
ultraviolet difference-spectroscopic studies of substrate and inhibitor binding to lactobacillus casei dihydrofolate reductase.the u.v. difference spectra generated when methotrexate, trimethoprim or folate bind to lactobacillus casei dihydrofolate reductase were analysed. the difference spectrum producted by methotrexate binding is shown to consist of three components: (a) one closely resembling that observed on protonation of methotrexate, reflecting an increased degree of protonation on binding; (b) a ph-independent contribution corresponding to a 40 nm shift to longer wavelengths of a single absorption band of metho ...197826334
lactostrepcins--acid bacteriocins produced by lactic streptococci.all 47 non-nisin producing strains of streptococcus lactis and 12/13 strains of str. lactis subsp. diacetylactis examined produced bacteriocins, for which the term lactostrepcins is suggested. seven strains of str. cremoris examined produced no bacteriocins active against 3 lactic streptococci strains used as indicators. the strains examined were divided into 3 groups: i, those producing lactostrepcins active against only one streptomycin resistant mutant of str. lactis 60 indicator strain; ii, ...197827542
dihydrofolate reductase from lactobacillus casei. x-ray structure of the enzyme methotrexate.nadph complex. 197829045
inability of selected lactobacilli to inhibit the heat-labile or heat-stable enterotoxin effects of escherichia coli b7a. 197829883
effect of caries-preventive measures in children with high numbers of s. mutans and lactobacilli. 197830166
extractability of cell wall polysaccharide from lactobacilli and streptococci by autoclaving and by dilue acid.autoclaving cell wall of streptococcus mutans ingbritt for 15 min under the rantz and randall conditions released one-tenth of the total cell wall carbohydrate, whereas two-thirds was extracted after autoclaving for 180 min. the extract contained the serotype c-specific antigen but lacked the lipoteichoic acid component extracted when whole cells were autoclaved. autoclaving cell wall preparations from other strains of s. mutans and also streptococcus salivarius and streptococcus mitis in 0.85% ...197832140
interrelationships between lactobacilli and streptococci in plaque formation on a tooth in an artificial mouth. 197832164
[guidelines for laevolac therapy against the endogenous factor in periodontal diseases]. 197833356
effect of growth conditions on the antigenic components of streptococcus mutans and lactobacilli.1) the release of lta from chemostat-grown cultures of s. mutans and l. fermentum is dependent on the generation time and ph. 2) the growth rate affects the immunogenicity of lta and the wall polysaccharide of l. fermentum. 3) the growth of l. plantarum under glucose limitation influences the glucose content of the wall teichoic acid. the composition of the wall polysaccharide of s. mutans ingbritt may also be changed under these conditions. 4) the conditions of autoclaving used to release cell ...197833543
thymidylate synthetase purified to homogeneity from human leukemic cells.thymidylate synthetase (5,10-methylenetetrahydrofolate:dump c-methyltransferase, ec 2.1.1.45) from a human leukemic cell line has been purified to homogeneity with one-step affinity column chromatography. the purified enzyme has a specific activity of 3.8 micron/min per mg of protein, which corresponds to a turnover number of 250. these are the highest values reported for a thymidylate synthetase from neoplastic tissue. a ratio of 1.7 mol of 5-fluoro-2'-deoxyuridylate binds per mol of enzyme in ...197934155
interpretation of nuclear magnetic resonance spectra for lactobacillus casei dihydrofolate reductase based on the x-ray structure of the enzyme-methotrexate-nadph complex.the three-dimensional molecular structure of lactobacillus casei dihydrofolate reductase complexed with nadph and methotrexate has been used to interpret published magnetic resonance spectra for this enzyme. proton resonances from histidine residues and 19f resonances from fluorine-labeled fluorotyrosine and fluorotryptophan dihydrofolate reductase have been assigned in several cases to specific amino acids in the primary sequence. furthermore, the 31p signals from the pyrophosphate moiety of bo ...197934432
circular-dichroism studies of ligand binding to dihydrofolate reductase from lactobacillus casei mtx/r.circular-dichroism spectra (200--450 nm) were recorded for lactobacillus casei mtx/r dihydrofolate reductase and its complexes with substrates, inhibitors and coenzymes. these spectra are compared with those reported by others for dihydrofolate reductase from other sources. the binding of nadp+ or nadph is associated with the perturbation of one or more aromatic amino acid residues, and there is marked enhancement of the negative c.d. band at 340 nm arising from the dihydronicotinamide chromopho ...197935152
dihydrofolate reductase from lactobacillus casei. stereochemistry of nadph binding.the nadph molecule binds to dihydrofolate reductase in an extended conformation. several of the individual dihedral angles, especially in the adenine mononucleotide portion of the coenzyme, differ from their minimum energy conformations. the ribose phosphate portions of the coenzyme are involved in numerous specific hydrogen-bonded and charge-charge interactions. the adenine ring resides in an apparently nonspecific hydrophobic cleft and the nicotinamide ring is bound within an intricately const ...197935535
effects of 3 months frequent consumption of hydrogenated starch hydrolysate (lycasin), maltitol, sorbitol and xylitol on human dental plaque.lozenges containing hydrogenated starch hydrolysate (lycasin), maltitol, sorbitol or xylitol were consumed 4 times daily during 3 months by 4 groups of persons (in all 85 subjects). in the maltitol-, sorbitol- and xylitol-group the plque wet weights were of the same magnitude before and after the test period. in the lycasin-group, a higher value was found after than before the 3-month period (p less than 0.01). the acid production in suspensions of dental plaque material from lycasin, maltitol a ...197935919
urease-producing species of intestinal anaerobes and their activities.urease activities of anaerobic bacteria that constituted predominant gut flora were examined. it was demonstrated that some strains of eubacterium aerofaciens, e. lentum, and peptostreptococcus products produced urease. they were the most numerous species in human feces. all strains of bifidobacterium infantis and some strains of bacteroides multiacidus, b. bifidum, clostridium symbiosum, fusobacterium necrophorum, f. varium, lactobacillus fermentum, peptococcus asaccharolyticus, and p. prevotii ...197936839
histidine decarboxylase from lactobacillus 30a: reconstitution from separated subunits. 197937890
aerobic and anaerobic cervical flora of healthy thai women.the bacteriologic study of the cervical flora in 50 healthy women revealed that 70% harbored mixed aerobic and anaerobic organisms. aerobic organisms alone were recovered in 15 women (30%). polymicrobial organisms were found in all but five women (in these only one aerobe was isolated per patient). the majority of patients had 1-2 anaerobes, with more than two aerobes. the common aerobes were alpha-hemolytic streptococci, staphylococcus epidermidis and lactobacilli. the most common anaerobes wer ...197839833
acidogenic fermentation of pentose alcohols by human dental plaque microorganisms. 197944186
chemotherapeutic effect of zinc on streptococcus mutans and rat dental caries. 197944465
the influence of environment on the oral flora of shangaan mineworkers. 197844502
effect of intestinal flagellate spironucleus (hexamita) muris and of dimetridazole on intestinal microflora in thymus-defficient (nude) mice.two groups of the intestinal microflora, the lactobacilli and the coliforms, were examined in thymus-deficient (nude) mice during the development of an experimental infection with the intestinal flagellate spironucleus (hexamita) muris and during the treatment with dimetridazole. the observed significant decrease in the number of lactobacilli under infection was probably due to the fact that the protozoan parasite fed on the microbes. dimetridazole (0.3% in drinking water) did not influence the ...197944620
serum cholesterol and bowel flora in the newborn.bottle-fed infants have a total serum cholesterol of 147 mg/100 ml on the fifth day of life and their stools contain more escherichia coli than lactobacilli organisms. the ratio of these bacteria can be reversed by adding bicarbonate or lactobacilli to feeds. this change is associated with a decrease in total cholesterol to a mean 119 mg/100 ml over the next 3 days. lactobacilli organisms predominate in the stools when serum cholesterol is low and may play a role in the metabolism of cholesterol ...197545573
lipoteichoic acids: a new class of bacterial antigen. 197546620
structural, functional, and idiotypic characteristics of a phosphorylcholine-binding iga myeloma protein of c57bl/ka allotype.an iga phosphorylcholine (pc)-binding myeloma protein with igch allotypic determinants different from those of balb/c mice is characterized. the myeloma, cbpc 2, was induced in the cb-20 strain of mice which is congenic to balb/c but differs from it by carrying the a15 allotypic determinant of c57bl/ka mice. sequence analysis of the cbpc 2 light chain through the first hypervariable region, as well as isoelectric point analysis, show that this chain is indistinguishable from that of t15, a pc-bi ...197546916
glycerol teichoic acid as an antigenic determinant in a gram-negative bacterium butyrivibrio fibrisolvens.an antigenic determinant isolated from a strain of the gram-negative bacterium butyrivibrio fibrisolvens reacted with specific antisera to the polyglycerophosphate backbone of membrane teichoic acids of lactobacilli. it gave a reaction of identity with membrane glycerol lipoteichoic acid and glycerol teichoic acid preparations from lactobacilli, and with phenol extracts of other gram-positive bacteria. the antigen-antibody reactions was strongly inhibited by glycerol-phosphoryl-glycerol-phosphor ...197550403
adhesion of lactobacilli to the chicken crop epithelium. 197550457
antigenic analyses of lactobacillus fermenti.antigenic analyses of lactobacillus fermenti were carried out by double immunodiffusion in agar using extracts prepared with cold trichloroacetic acid (tca) or hot dilute hydrochloric acid (hcl). a common antigen of l. fermenti, designated as antigen f by the author, was extracted from whole cells with dilute hcl, but not with tca. the antigen f was also observed in lactobacillus casei. in addition, all strains isolated from human saliva contained antigen 6 in their cell walls, while the antigen ...197550468
grouping and cross-reacting antigens of oral lactic acid bacteria.the grouping antigens from five of the seven serological groups of lactobacilli have been defined with respect to the carbohydrate units responsible for serological specificity. when the antigens are polysaccharides, specificity is more absolute than with the teichoic acids where cross-reactions may occur because of the same or similar carbohydrate substituents, or more particularly because of the common "backbone" structure of the glycerol teichoic acids. this glycerol phosphate "backbone" acco ...197654372
cotrimoxazole and folate metabolism.cotrimoxazole 4 tablets daily (1 tablet = trimethoprim 80 mg and sulphamethoxzole 400 mg) was given for a period of six to fourteen days to 13 inpatients, and serum-folate levels were measured before and one day after the course of treatment. the results were compared with those from 8 patients not receiving antibacterial therapy, tested on admission and one week later. two assay techniques were used, one employing lactobacillus casei and the other 125i-labelled folate isotope dilution. the micr ...197660570
grouping antigens of four lactobacillus species and their characteristics.antigenic analyses of lactobacillus bulgaricus, lactobacillus lactis, lactobacillus brevis and lactobacillus buchneri were carried out by double immunodiffusion in agar. antigens were extracted from whole cells and cell wall preparations with cold trichloroacetic acid. most strains of the four species possessed antigen 9 in their cell walls. another antigen, antigen 10, was found in the cell walls of all the strains of l. brevis and l. buchneri, and in some strains of l. lactis, but not in l. bu ...197662862
aldolases of the lactic acid bacteria. demonstration of immunological relationships among eight genera of gram positive bacteria using an anti-pediococcal aldolase serum.reciprocal qualitative and quantitative immunological experiments employing an anti-pediococcus cerevisiae aldolase serum confirmed many of the interspecific relationships demonstrated previously among lactic acid bacteria with antisera prepared against the streptococcus faecalis fructose diphosphate aldolase. the extent of immunological relatedness observed between the lactobacillus and pediococcus aldolases was markedly gses indicating that the pediococci share closer phylogenetic ties with th ...197665160
casein degradation and amino acid liberation in milk by two highly proteolytic strains of lactic acid bacteria.amongst 100 isolates of lactic acid bacteria, lactobacillus bulgaricus and streptococcus feacalis proved to be highly proteolytic as demonstrated by starch gel electrophoresis and liberation of various amino acids. both these organisms hydrolysed casein differently to produce different concentrations of amino acids.197770973
[simple method of growing sporeless anaerobic microorganisms on flat media]. 197772883
specific antigens of lactobacillus acidophilus.antigens specific for lactobacillus acidophilus were investigated by double immunodiffusion in agar-gel. antigenic materials were extracted from whole bacteria and some walls with cold trichloroacetic acid. antisera were prepared by intravenous injection into rabbits of suspensions of whole organisms in solutions of bovine serum albumin, which had been heated and then washed. four specific antigens were found as precipitinogens and denoted as antigens 11, 12, 13 and 14. of 43 strains of l. acido ...197774399
fluorescent pigments in the newly isolated methylotrophs: pseudomonas j16 and methylomonas pl1.the pigments showing fluorescence maxima at 390, 366, 450-460 and 520 nm at excitation wavelength 254, 366 and 450 nm respectively, were detected in the cells and culture media of the obligate methylotroph methylomonas pl1 and facultative methylotroph pseudomonas j26. the maximum at 520 nm is associated with the occurrence of a flavin pigment enabling growth of lactobacillus casei e atcc-7469 on the vitamin b2 deficient medium. the remaining fluorescence maxima are related to the prosthetic grou ...197881599
the puzzle of "urethral syndrome": a possible answer?82 strains of slow-growing, co2-dependent, gram-positive organisms have been isolated from the urine of 9 male and 73 female patients. 100% of the men and 93% of the women had urinary symptoms at the time of isolation, and 66% of the specimens showed pyuria. these organisms might account for the urinary symptoms of some patients previously diagnosed as having "urethral syndrome". preliminary data show that, if appropriate antibacterial treatment is given, most of these patients become symptom-fr ...197986778
lactobacillus overgrowth for treatment of moniliary vulvovaginitis. 197989552
lactobacillus for vulvovaginitis. 197990880
aflatoxin b1 effect on beta-d-galactosidase in some starter cultures.six starter cultures were examined for their ability to induce beta-d-galactosidase activity. the maximum (713 x 10(-4) units/ml) and minimum (140 x 10(-4) units/ml) enzyme activity was shown by lactobacillus bulgaricus and streptomyces thermophilus, respectively. the maximum inhibition by aflatoxin b1 of induction and activity of enzyme in vitro was observed at 60 micrograms/ml in all cultures. however, low levels of the toxin stimulated induction of the enzyme in streptococcus lactis and s. th ...197992172
ultrastructure and partial characterization of a regular array in the cell wall of lactobacillus brevis.the cell wall of lactobacillus brevis was revealed by electron microscopy to have an outer layer composed of a regular array. the morphological unit of the regular array appeared to consist of four spherical subunits, each about 2 nm in diameter, which were arranged in a tetragonal pattern about 4.5 by 7.0 nm in dimension. the regular array was composed of the tetragonal units in rows in two directions at an angle of about 75 degrees to each other. the average spacing between the rows was about ...197992747
identification of bacteroides fragilis by indirect immunofluorescence.rabbit antiserum against live or heat-killed bacteroides fragilis nctc 9343 bacteria was titrated against hot phenol water-extracted polysaccharide antigens from five different species of the 'b. fragilis group' of bacteria using an enzyme immunoassay and shown to be specific for the b. fragilis nctc 9343 polysaccharide. when the antiserum was used in indirect immunofluorescence, 97.1% of 244 b. fragilis strains were correctly identified. only 8 of the other 312 bacteroides strains were stained ...197994651
vegetarian vs. normal as casual agents in tooth decay. 197796194
essential arginyl residues in thymidylate synthetase. 197896829
folate status during long-term therapy with trimethoprim and sulphadiazine.it was studied whether treatment with sulphadiazine and trimethoprim for 8 weeks affected the folate status. the therapy did not inhibit the absorption as assessed by the 3h--folic acid absorption (trifa) test. plasma folic acid clearance showed that folate deficiency had not developed. bone marrow studies revealed that the antifolic action of the drugs did not manifest itself at the cellular level. the lactobacillus casei method should not be employed in the determinations of the serum folate c ...197897059
lactase activity of microorganisms.sixty-two strains of yeasts, molds and bacteria were screened for lactase (beta-d-galactosidase) activity. strains exhibiting the enzyme activity were evaluated for cell yield as well as enzyme units available per litre of the medium, per g cell dry weight and per mg protein of their cell-free extracts. the molds exhibited lowest enzyme activity but highest cell yields, bacteria produced lowest cell yield and maximum enzyme activity. cultures exhibiting very high activity among yeasts were sacch ...197897187
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