| [neutral and basic compounds present in gases produced by clostridium sporogenes, plectridium putrificum and plectridium glycolicum cultured under vacuum in sodium thioglycolate glucose broth]. | the study of gases produced by clostridium sporogenes, plectridium putrificum et p. glycolicum cultured under vacuum in sodium thioglycolate glucose broth makes possible the detection of compounds not described before for these bacteria, particularly methyl-2 butanal and methyl-3 butanal for p. glycolicum and thiacyclopropane in these 3 bacteria. thiacyclopropane is the result of the cyclization of mercapto-2 ethanol produced by the reduction of thioglycolic acid. | 1982 | 6817866 |
| egg-yolk trypticase soy agar for the enumeration of heat-damaged spores of clostridium sporogenes. | in heat-resistance studies with spores of clostridium sporogenes bc-2, an improved recovery medium was needed for severely heat-damaged spores as the used previously--wynne medium in miller-prickett tubes--did not allow accurate counts of spores because of gas formation and disruption of agar. initial test with pour plates of viande-leyure medium containing egg-yolk gave much increased counts for spores previously heated for 50 min at 112 degrees c; this increase was attributed to the presence o ... | 1983 | 6228543 |
| involvement of zinc in regulatory properties of arginine deiminase from clostridium sporogenes. | | 1980 | 6778815 |
| production of hydrocinnamic acid by clostridia. | hydrocinnamic acid was found in acid extracts of spent growth medium from cultures of clostridium sporogenes. the acid was identified by mass spectrometry and its identity was confirmed by gas chromatography. the acid was produced in relatively large amounts (2 to 3 mumoles/ml of medium) by c. sporogenes, toxigenic types a, b, d, and f of c. botulinum, and some strains of c. bifermentans. other strains of c. bifermentans and strains of c. sordellii and c. caproicum produced only small amounts (0 ... | 1970 | 5437307 |
| diet as a coadjuvant for development of antibiotic-associated diarrhea in hamsters (mesocricetus auratus). | during a study of the effects of nutrition on experimental tumors in hamsters, fatal diarrhea developed. to determine the role of diet in this condition and the relationship between diet and antibiotics, two diets were used, ground commercial diet and a purified diet. two antibiotics were used, neomycin sulfate and vancomycin. diarrhea was evident soon after the animals were given the combination of purified diet and neomycin sulfate. vancomycin initially acted as a suppressor of diarrhea, but h ... | 1981 | 6790835 |
| effect of glucose concentration in the growth medium upon neutral and acidic fermentation end-products of clostridium bifermentans, clostridium sporogenes and peptostreptococcus anaerobius. | clostridium bifermentans, c. sporogenes and peptostreptococcus anaerobius were grown in fastidious anaerobe broth with glucose concentrations from 0-1.5% (w/v). gas chromatographic (glc) analysis of fermentation end-products revealed that a change in glucose concentration markedly affected fermentation patterns of each microorganism. increased glucose concentration resulted in stimulation of ethanol production by all strains, and of butanol and butyric acid by c. sporogenes. with c. bifermentans ... | 1983 | 6822993 |
| tryptophan transaminase from clostridium sporogenes. | | 1968 | 5697992 |
| immunodiffusion method for detection of type a clostridium botulinum. | a simple gel immunodiffusion agar procedure was developed for detecting toxigenic strains of clostridium botulinum type a. the method consisted of overlaying colonies grown on thin-layer tryptone-peptone-glucose-yeast extract agar with gel diffusion agar containing desired levels of c. botulinum type a antitoxin. concentric precipitin zones formed around colonies of c. botulinum type a. strains of c. botulinum type a were detected by this procedure. however, c. botulinum type b reacted to a less ... | 1981 | 6797350 |
| the involvement of nitric oxide in the inhibition of the phosphoroclastic system in clostridium sporogenes by sodium nitrite. | the phosphoroclastic system was demonstrated in cell-free extracts of clostridium sporogenes by the production of carbon dioxide, acetyl phosphate, atp and reduced nad in the presence of pyruvate. the kinetics of acetyl phosphate production and nad reduction were investigated. the addition of sodium nitrite to a suspension of c. sporogenes in glucose medium resulted in a rapid decrease in intracellular atp concentration which was accompanied by an accumulation of pyruvate in the medium. this acc ... | 1981 | 6798167 |
| purification and partial characterization of proline dehydrogenase from clostridium sporogenes. | a proline dehydrogenase which catalyzes the nicotinamide adenine dinucleotide (nad) dependent oxidation of proline and the nadh-dependent reduction of delta 1-pyrroline 5-carboxylic acid (pca) was purified from extracts of clostridium sporogenes. following purification, only one protein band was found on analytical polyacrylamide disc gels and on sodium dodecyl sulfate (sds) - polyacrylamide disc gels. sucrose density gradient centrifugation and sds-gel electrophoresis indicated that the enzyme ... | 1981 | 6895480 |
| microbiological aspects of ethylene oxide sterilization. ii. microbial resistance to ethylene oxide. | the death rate kinetics of several sporeforming and nonsporeforming microorganisms, including radiation-resistant cocci, were determined by exposing them to a mixture of ethylene oxide and dichlorodifluoromethane (500 mg of ethylene oxide per liter, 30 to 50% relative humidity, and 54.4 c). spore survivor curves obtained from tests of inoculated and exposed hygroscopic and nonhygroscopic carriers showed that the spores of bacillus subtilis var. niger are more resistant to ethylene oxide than are ... | 1970 | 5415211 |
| characterization of clostridium difficile and its differentiation from clostridium sporogenes by automatic head-space gas chromatography. | although 47 strains of clostridium difficile and clostridium sporogenes were studied by gas chromatography. acidic and neutral volatile products, formed after 96 h of incubation in glucose-containing peptone yeast-extract medium, were chromatographed. all strains produced appreciable amounts of fatty acids, which were tentatively identified by gas chromatographic retention data. chromatograms obtained when analysing diethyl ether extracts of culture media of all 47 strains were virtually identic ... | 1980 | 6937946 |
| appraisal of anotox, a new anaerobic atmospheric detoxifying agent for use in anaerobic cabinets. | using clostridium sporogenes cultures, inhibitory effects of its gaseous metabolic end products on the activity of palladium deoxo catalyst and on surface growth of some clinically significant anaerobes were demonstrated. a new product "anotox" was shown to absorb these metabolites, which enabled uninhibited growth of anaerobes, and prolonged the life of the catalyst. | 1982 | 7068916 |
| [differentiation of clostridium sporogenes and clostridium bifermentans-clostridium sordelli using agar gel pricipitation]. | | 1967 | 6079138 |
| [in vitro study of the lipase activity of bacterial strains isolated from the rabbit cecum on c14-labeled triolein]. | the lipase activity of bacterial species isolated from the caecum of conventional rabbits was studied by means of two groups of tests: (1) bacteriologic isolation and an identification of nine aerobic and anaerobic clones and (2) incubation of each of these clones in the appropriate medium in the presence of labelled triolein (oleyl 1-14c), a biochemical analysis of the radioactivity of the fractions obtained. the main results can be expressed in the following way: maximum hydrolysis of glycerid ... | 1983 | 6405674 |
| glucono-delta-lactone and citric acid as acidulants for lowering the heat resistance of clostridium sporogenes pa 3679 in htst working conditions. | the heat resistance of clostridium sporogenes pa 3679 spores has been studied to establish the influence of acidification with glucono-delta-lactone (gdl) and citric acid on the thermal resistance parameters (dt and z) of this microorganism and to compare their effect with phosphate buffer and natural asparagus as reference substrates. a reduction in dt values was observed in asparagus purée as the acidification level increased with both acidulants although this effect was more evident at the lo ... | 1995 | 7547150 |
| some properties of bacteriophages active on the obligate anaerobe clostridium sporogenes. | | 1968 | 5669990 |
| utilization of cheddar cheese containing nisin as an antimicrobial agent in other foods. | cheddar cheese made with nisin-producing lactococci contained between 400 and 1200 iu of nisin per gram of cheese. cultures used were lactococcus lactis ssp. cremoris js102, a nisin-producing transconjugant developed in the laboratories of dr. l.l. mckay and lactococcus lactis ssp. lactis ncdo 1404 obtained from the national collection of food bacteria, reading, england. pasteurized process cheese spreads with 53% and 60% moisture and 0, 301 and 387 iu nisin/g were manufactured and inoculated wi ... | 1994 | 7703016 |
| the effect of formulation of parenteral solutions on microbial growth--measurement of d- and z-values. | in order to assess the sterilization conditions for parenteral solutions, the microbial inactivation kinetics of steam resistant spores of clostridium sporogenes were evaluated. the d-value or death rate kinetics as well as z-values were obtained for several formulation categories of solutions employing a miniature steam retort. amino acid and heparin solutions provided appreciable microbial resistance as demonstrated by resulting high d- and low z-values. the addition of electrolytes to carbohy ... | 1995 | 7757457 |
| [rumen bacteria degrading toxic mimosine and dihydroxypyridine compounds in china]. | four anaerobic strains were isolated from the rumen of the cattle which no specific toxic symptoms were seen when leucaena was fed in weizhou island beihai city, guangxi province, china. all of these strains (br-1, br-2, br-5 and br-7) possess degradative activities to toxic mimosine, 3-hydroxy-4 (1h) -pyridone (3, 4-dhp) and 2, 3-dihydroxypyridine (2, 3dhp) from leucaena, that were confirmed by analysis of hplc. pure and mixed cultures of these four strains in vitro degraded 44-59% of mimosine, ... | 1994 | 7871781 |
| antimicrobial activity of lactobacilli: preliminary characterization and optimization of production of acidocin b, a novel bacteriocin produced by lactobacillus acidophilus m46. | approximately 1000 lactobacillus strains were isolated and screened for the production of antimicrobial activity, using a target panel of spoilage organisms and pathogens. only eight positive strains were found; two of these were studied in more detail. lactobacillus salivarius m7 produces the new broad spectrum bacteriocin salivaricin b which inhibits the growth of listeria monocytogenes, bacillus cereus, brochothrix thermosphacta, enterococcus faecalis and many lactobacilli. a new atypical bac ... | 1994 | 7961186 |
| antimicrobial activity of essential oil from schinus molle linn. | the essential oil from the fresh leaves of schinus molle isolated by hydrodistillation was tested for antibacterial activity using the hole plate diffusion method and for antifungal activity using the mycelium or single cell growth inhibition method. results obtained showed that the volatile oil exhibited significant activity against the following bacterial species: klebsiella pneumoniae, alcaligenes faecalis, pseudomonas aeruginosa, leuconostoc cremoris, enterobacter aerogenes, proteus vulgaris ... | 1993 | 8055554 |
| sporicidal activity of chemical sterilants used in hospitals. | the current study was designed to assess the sporicidal activity of chemical sterilants using the association of official analytical chemists (aoac) sporicidal test. chemical sterilants are used most commonly in the healthcare setting to disinfect medical instruments such as endoscopes. this study was undertaken to evaluate the efficacy of several newer formulations of glutaraldehydes and hydrogen peroxide as chemical sterilants. | 1993 | 8132997 |
| characteristics of toxicity and haemorrhagic toxin produced by clostridium sporogenes in various animals and cultured cells. | the toxic effects of the haemorrhagic toxin of clostridium sporogenes were studied in mice, rats, guinea-pigs and rabbits, and in various cultured cells. in rabbits, but not in the other animals, intradermal injection with crude toxin and its injection into a ligated intestinal loop caused haemorrhage in both the skin and intestinal wall. intraperitoneal (i.p.) injection of crude toxin similarly caused death only of rabbits, with marked haemorrhage in the serous surface of kidney, intestines, li ... | 1997 | 9128190 |
| effect of microwave radiation on inactivation of clostridium sporogenes (pa 3679) spores. | three techniques for studying effects of microwave radiation on microorganisms were introduced. spores of clostridium sporogenes (pa 3679) were chosen as a test organism because the kinetic parameters for thermal inactivation are well known and because of the importance of the genus clostridium to the food industry. for the first technique, a specially designed kinetics vessel was used to compare inactivation rates of microwave-heated and conventionally heated spores at steady-state temperatures ... | 1994 | 8135512 |
| inactivation of clostridium difficile spores by disinfectants. | the current study was designed to evaluate the activity of glutaraldehyde-based disinfectants against clostridium difficile using the association of official analytical chemists' (aoac) sporicidal test. this study was undertaken because gastrointestinal endoscopes that may be contaminated with c difficile spores are most commonly disinfected between patients using glutaraldehyde-based disinfectants. | 1993 | 8432966 |
| growth of listeria monocytogenes and clostridium sporogenes in cottage cheese in modified atmosphere packaging. | low fat cottage cheese (ph 5.14) was inoculated with three strains of listeria monocytogenes, serotypes 1a and 4b, an isolate from a dairy processing plant, and clostridium sporogenes atcc 3584. the cheese was packaged with or without added dissolved co2 in polystyrene tubs overwrapped with or without high barrier heat shrink film and stored at 4, 7, and 21 degrees c for up to 63 d. the concentration of co2 in the container headspace was 35% (vol/vol). the co2 concentration in that headspace dec ... | 1993 | 8486848 |
| the antimicrobial properties of chile peppers (capsicum species) and their uses in mayan medicine. | a survey of the mayan pharmacopoeia revealed that tissues of capsicum species (solanaceae) are included in a number of herbal remedies for a variety of ailments of probable microbial origin. using a filter disk assay, plain and heated aqueous extracts from fresh capsicum annuum, capsicum baccatum, capsicum chinese, capsicum frutescens, and capsicum pubescens varieties were tested for their antimicrobial effects with fifteen bacterial species and one yeast species. two pungent compounds found in ... | 1996 | 8735449 |
| feasibility of automated head-space gas chromatography in identification of anaerobic bacteria. | neutral and acidic metabolites of some anaerobic bacteria cultured in a liquid growth medium were analysed by gas chromatography. studies on the head-space vapours above heated broth cultures, using a gas chromatograph equipped with a fused silica capillary column and a unit for automatic head-space injection, enabled volatile alcohols and fatty acids to be readily detected. by contrast, when studying ether extracts of the same cultures, using a packed column, alcohols were only rarely registere ... | 1982 | 7080829 |
| [synthetic medium for culturing clostridium sporogenes]. | clostridium sporogenes was shown to be capable of good stable-growth and biomass accumulation up to 5 mg/ml (dry weight) in a chemically defined medium. along with glucose, the culture required the following amino acids for its growth: arginine, phenylalanine, glycine tyrosine, leucine, isoleucine, valine, tryptophan, and methionine. the culture could grow in the absence of inorganic nitrogen but required such elements as phosphorus, iron and magnesium. addition of cysteine to the medium acceler ... | 1982 | 7087816 |
| interconversion of valine and leucine by clostridium sporogenes. | clostridium sporogenes has been found to require l-leucine and l-valine for growth in a minimal medium, although valine can be replaced by isobutyrate and leucine by isovalerate. cells grown in minimal media incorporated significant 14c from [14c]valine into leucine and from [14c]leucine into valine. growth with [4,5-3h]leucine also resulted in the incorporation of 3h into valine. these results indicate that these bacteria can interconvert leucine and valine. | 1982 | 7130135 |
| the stereochemical course of the water elimination from (2r)-phenyllactate in the amino acid fermentation of clostridium sporogenes. | (2r,3r)-[3-3h]- and (2r,3s)-[3-3h]- phenyllactate were synthesized from phenylpyruvate with the help of phenylpyruvate tautomerase and an nadh-dependent 2-oxo-acid reductase from clostridium sporogenes. with whole cells of c. sporogenes both (2r)-phenyllactates are mainly transformed to phenylpropionate and, to a minor extent to phenylalanine. according to recently published results, the former transformation leads from phenyllactate, or an activated derivative of it, to (e)-cinnamate which is r ... | 1982 | 7141406 |
| nisin: a possible alternative or adjunct to nitrite in the preservation of meats. | nisin at 75 ppm (75 microgram/g) was superior to 150 ppm of nitrite in inhibiting outgrowth of clostridium sporogenes pa3679 spores in meat slurries, which had been heated to simulate the process used for cooked ham. the inhibitory activity of nisin decreased as the spore load or ph of the slurries increased. unlike nitrite, inhibition by nisin was unaffected by high levels of iron either as a constituent of meats or when added as an iron salt. in slurries treated with 75 ppm of nisin, refrigera ... | 1981 | 7195188 |
| on the formation of 3-phenylpropionate and the different stereo-chemical course of the reduction of cinnamate by clostridium sporogenes and peptostreptococcus anaerobius. | | 1981 | 7202724 |
| bacterial profile of ground beef made from carcass tissue experimentally contaminated with pathogenic and spoilage bacteria before being washed with hot water, alkaline solution, or organic acid and then stored at 4 or 12 degrees c. | the long-term effectiveness of several beef-carcass surface-tissue (bct) wash interventions on the microbiology of ground beef produced from this tissue was determined. bct was inoculated with bovine feces containing one of two different levels (ca. 4 or 6 log cfu/ml) of escherichia coli o157:h7, listeria innocua, salmonella typhimurium, and clostridium sporogenes. the bct was then subjected to one of several treatment washes: 2% (vol/vol) dl-lactic acid (la), 2% (vol/vol) acetic acid (aa), 12% ... | 1998 | 9766060 |
| influence of growth conditions on glycine reductase of clostridium sporogenes. | cells of clostridium sporogenes were deficient in glycine reductase activity when grown in a rich medium containing 40 mm each of exogenously added pyruvate and proline or hydroxyproline. these cells lacked the selenoprotein and at least one more protein of the glycine reductase system. proline or hydroxyproline in the medium also influenced the uptake of glycine by the cells. | 1980 | 7354004 |
| association of vegetative antigens with the spores of clostridium sporogenes. | clean spores of clostridium sporogenes stored for two years continued to elicit the production of specific antibodies in immunized rabbits. disintegrated spores stimulated not only antibodies specific for the spore component, but also those directed against 'h' and 'o' antigens of the sporangium cells. | 1980 | 6781821 |
| effect of moderately acidic ph on heat resistance of clostridium sporogenes spores in phosphate buffer and in buffered pea puree. | the effect of ph in the range 5.0 to 7.0 on the thermal destruction of spores of clostridium sporogenes putrefactive anaerobe 3679 was examined by three methods: a capillary tube method in which spores were suspended in phosphate buffers, a thermoresistometer method in which spores were suspended in buffered pea puree adjusted to the same set of ph values, and a thermal death time can method in which spores were again suspended in buffered pea puree. the results indicated that increasing acidity ... | 1980 | 7396485 |
| salt extends the upper temperature limit for growth of food-poisoning bacteria. | inclusion of nacl into the growth medium raised the upper temperature limit of growth of the following organisms: staphylococcus aureus (two strains), salmonella senftenberg, s. typhimurium, escherichia coli, streptococcus faecalis, bacillus cereus, clostridium sporogenes, c. perfringens (two strains). the magnitude of the response varied with the culture, the largest being 3.5 degrees with b. cereus cells. the spores of b. cereus were not protected by salt but clostridial spores behaved as the ... | 1981 | 6796247 |
| purification and some properties of clostridium sporogenes hemorrhagic toxin. | clostridium sporogenes was isolated from rabbits with antibiotic-associated hemorrhagic diarrhea, then its hemorrhagic toxin was purified from the supernatant by means of hydrophobic interaction chromatography, hydroxyapatite chromatography, and gel filtration. the purified toxin's hemorrhagic activity was completely inhibited by edta, but not by pmsf or ovomucoid; and fully restored by the addition of such divalent cations as zn2+, ca2+ and mg2+. the purified toxin did not hydrolyze azocasein, ... | 1996 | 8878529 |
| mathematical model for the combined effect of temperature and ph on the thermal resistance of bacillus stearothermophilus and clostridium sporogenes spores. | two mathematical models have been studied to establish the relationship between the ph, treatment temperature and thermal destruction constant (k) of bacillus stearothermophilus and clostridium sporogenes spores. the study was carried out by heating the spores in mushroom extract acidified with two different acidulants (citric acid and glucono-delta-lactone). among the models studied, the one that best described the inactivation was a second order polynomial equation, the precision of which depe ... | 1996 | 8880342 |
| production of indole-3-propanoic acid and 3-(p-hydroxyphenyl)propanoic acid by clostridium sporogenes: a convenient thin-layer chromatography detection system. | indole-3-propanoic acid (ipa), 3-(p-hydroxyphenyl)propanoic acid (hppa), and 3-phenylpropanoic acid (ppa) were present in the spent bacterial media of clostridium sporogenes (20/20 strains) and c. cylindrosporum (1/1 strains), but absent in 32 other clostridial species (74 strains) tested. both ipa and hppa (but not ppa) could be readily detected by thin-layer chromatography and p-hydroxybenzaldehyde spray reagent. ipa forms a scarlet complex with p-hydroxybenzaldehyde which shifts to purple and ... | 1980 | 7378938 |
| incidence of diarrhea with antibiotics and the increase of clostridia in rabbits. | rabbits were treated with a single intravenous injection of various antibiotics. more than 40 per cent of the animals showed diarrhea after being treated with sulbactam/cefoperazone, cefmetazole, clindamycin, piperacillin or aspoxicillin. clostridium difficile was isolated from sulbactam/cefoperazone-treated diarrheic rabbits, with their cecal contents showing positive reaction in a latex agglutination test for c. difficile enterotoxin. however, 27 cefmetazole-induced diarrheic cases were not as ... | 1996 | 8996700 |
| monoclonal antibodies for use in detection of bacillus and clostridium spores. | five monoclonal antibodies against bacterial spores of bacillus cereus t and clostridium sporogenes pa3679 were developed. two antibodies (b48 and b183) were selected for their reactivity with b. cereus t spores, two (c33 and c225) were selected for their reactivity with c. sporogenes spores, and one (d89) was selected for its reactivity with both b. cereus and c sporogenes spores. the isotypes of the antibodies were determined to be immunoglobulin g2a (igg2a) (b48), igg1 (b183), and igm (c33, c ... | 1997 | 9023926 |
| effect of hemorrhagic toxin produced by clostridium sporogenes on rabbit ligated intestinal loop. | in order to characterize the toxicity of the hemorrhagic toxin of clostridium sporogenes isolated from the rabbit with antibiotic-associated hemorrhagic diarrhoea, we studied the toxin productivity of c. sporogenes cultured in various media and the toxic effect of the partially purified preparation of the culture supernatant in rabbit intestinal loops. the hemorrhagic activity, which was determined by rabbit skin test for assessment of the toxin production, of culture supernatant of c. sporogene ... | 1997 | 9032760 |
| the role of mammalian intestinal bacteria in the reductive metabolism of zonisamide. | zonisamide (1,2-benzisoxazole-3-methanesulphonamide), a new anticonvulsant, is mainly metabolized to 2-sulphamoylacetylphenol by reduction of the benzisoxazole ring. recent studies have shown that mammalian liver enzymes are responsible for the reduction of zonisamide. because intestinal bacteria can also mediate the reduction of xenobiotics, this study was designed to evaluate the role of intestinal bacteria in in-vivo reductive metabolism of zonisamide. treatment of rats with antibiotics signi ... | 1997 | 9231340 |
| exosporial membrane plasticity of clostridium sporogenes and clostridium difficile. | this investigation examines the morphological alterations of the exosporial membranes of clostridium sporogenes atcc 3584 and clostridium difficile atcc 43594 and 9689 endospores in relation to their possible function during germination in the attachment/colonization process of these pathogenic bacteria. there is no reported function for the exosporial membrane, nor exosporial appendages, of clostridial endospores. advances in high resolution, scanning electron microscopy (sem) permit the examin ... | 1997 | 9281845 |
| effects of high hydrostatic pressure on clostridium sporogenes spores. | spores of clostridium sporogenes were found to be resistant to ultra high pressure, with treatments of 600 mpa for 30 min at 20 degrees c causing no significant inactivation. combination treatments including heat and pressure applied simultaneously (e.g. 400 mpa at 60 degrees c for 30 min) or sequentially (e.g. 80 degrees c for 10 min followed by 400 mpa for 30 min) proved more effective at inactivating spores. pressure cycling (e.g. 60 mpa followed by 400 mpa at 60 degrees c) also reduced spore ... | 1998 | 9569715 |
| thiaminase activity amongst strains of clostridium sporogenes. | | 1980 | 7462122 |
| genetic analysis of acidocin b, a novel bacteriocin produced by lactobacillus acidophilus. | the genes encoding the production of acidocin b, a bacteriocin produced by lactobacillus acidophilus strain m46 which is active against listeria monocytogenes, clostridium sporogenes, brochothrix thermosphacta, lactobacillus fermentum and lactobacillus delbrueckii subsp. bulgaricus, but inactive against most other lactobacillus species, were previously localized on a 4 kb xbai-hindiii fragment of plasmid pcv461. in the present work, dna sequence analysis revealed the presence of three consecutiv ... | 1995 | 7551031 |
| case report: empyema with hydropneumothorax and bacteremia caused by clostridium sporogenes. | clostridia species are rare causes of pleuropulmonary infections. this report describes an immunocompromised patient who had a renal transplant, had multiple risk factors for anaerobic pleuropulmonary infection, and developed an acute empyema with hydropneumothorax that was associated with clostridium sporogenes bacteremia. therapy included antibiotics and surgical drainage of the empyema. species identification of clostridia can usually be limited to whether the species is perfringens or nonper ... | 1996 | 8900389 |
| the effect of closure processing on the microbial inactivation of biological indicators at the closure-container interface. | two biological indicators are routinely used by the hospital products division to demonstrate the sterilization of the closure-container interface. the use of a moist heat (clostridium sporogenes) and a dry heat (bacillus subtilis) biological indicator allows a better understanding of the parameters that impact sterilization of the closure-container system. the ability to sterilize a given closure-container interface is defined in large part by closure moisture and product time above 100 degrees ... | 1998 | 9610171 |
| discussion of the z-value to use in calculating the f0-value for high-temperature sterilization processes. | the appropriate z-value to use in integrating heat process time-temperature data in the temperature range of 120.0-140.0 degrees c (248.0-284.0 degrees f) is discussed. we conclude that for control of clostridium botulinum there is little risk in extrapolating a public health f0-value for c. botulinum to temperatures in the 132.0-138.0 degrees c (270.0-280.0 degrees f) range using a z-value of 10.0 degrees c (18.0 degrees f). it would seem prudent, at this time, when extrapolating data to condit ... | 1996 | 8846059 |
| characterization of natural isolate lactobacillus acidophilus bgra43 useful for acidophilus milk production. | lactobacillus acidophilus bgra43 was selected from a set of human origin isolates of lact. acidophilus strains for the highest growth rates and antagonistic effect against both gram-positive and gram-negative bacteria. the strain bgra43 also exhibited an inhibitory effect on the growth of clostridium sporogenes. inhibition of this strain seems to be due to lactic acid production rather than hydrogen peroxide or bacteriocin. growth of lact. acidophilus bgra43 in non-fat skim milk for 6 h at 37 de ... | 1998 | 9633657 |
| long-term effect of alkaline, organic acid, or hot water washes on the microbial profile of refrigerated beef contaminated with bacterial pathogens after washing. | the effect of 2% (vol/vol) lactic acid, 2% (vol/vol) acetic acid, 12% (wt/vol) trisodium phosphate, water at 72 degrees c and water at 32 degrees c washes on bacterial populations introduced onto beef carcass surfaces after treatment was determined for up to 21 days at 4 degrees c storage in vacuum packaging. beef carcass short plates were collected from cattle immediately after slaughter and subjected to the above treatments or left untreated (c). short plates were then inoculated with low leve ... | 1998 | 9708300 |
| isolation, purification and partial characterization of plantaricin 423, a bacteriocin produced by lactobacillus plantarum. | lactobacillus plantarum 423, isolated from sorghum beer, produces a bacteriocin (plantaricin 423) which is inhibitory to several food spoilage bacteria and food-borne pathogens, including bacillus cereus, clostridium sporogenes, enterococcus faecalis, listeria spp. and staphylococcus spp. plantaricin 423 is resistant to treatment at 80 degrees c, but loses 50% of its activity after 60 min at 100 degrees c and 75% of its activity after autoclaving (121 degrees c, 15 min). plantaricin 423 remains ... | 1998 | 9717299 |
| the effect of essential oils of basil on the growth of aeromonas hydrophila and pseudomonas fluorescens. | basil essential oils, including basil sweet linalool (bsl) and basil methyl chavicol (bmc), were screened for antimicrobial activity against a range of gram-positive and gram-negative bacteria, yeasts and moulds using an agar well diffusion method. both essential oils showed antimicrobial activity against most of the micro-organisms examined except clostridium sporogenes, flavimonas oryzihabitans, and three species of pseudomonas. the minimum inhibitory concentration (mic) of bmc against aeromon ... | 1998 | 9633630 |
| fibronectin and laminin binding of eighteen clostridium species. | the ability of clostridium difficile, clostridium perfringens, clostridium sporogenes and fifteen other clostridium species to bind to human serum fibronectin or laminin was tested by using protein-coated latex particles. three groups of clostridium species were formed, namely the pseudomembranous colitis-causing species clostridium difficile, the gas gangrene-causing clostridium species and other clostridium species, which are infrequently found in human infections. significantly more strains o ... | 1995 | 9810669 |
| purification and characterization of thermophilin t, a novel bacteriocin produced by streptococcus thermophilus aca-dc 0040. | aca-dc 0040 produced an antimicrobial agent, which was named thermophilin t, active against several lactic acid bacteria strains of different species and food spoilage bacteria, such as clostridium sporogenes c22/10 and cl. tyrobutyricum ncdo-1754. the crude antimicrobial compound is sensitive to proteolytic enzymes and alpha-amylase, heat-stable (100 degrees c for 30 min), resistant to ph exposure at ph 1-12 and demonstrates a bactericidal mode of action against the sensitive strain lactococcus ... | 1998 | 9633654 |
| antibacterial activity of lactobacillus plantarum ug1 isolated from dry sausage: characterization, production and bactericidal action of plantaricin ug1. | lactobacillus plantarum ug1 isolated from dry sausage produced an antimicrobial substance that inhibited other strains of the genera lactobacillus and lactococcus, and some foodborne pathogens including listeria monocytogenes, bacillus cereus, clostridium perfringens and clostridium sporogenes. this antibacterial substance was inactivated by proteolytic enzymes and showed a bactericidal mode of action. consequently, it was characterized as a bacteriocin, and was designated plantaricin ug1. this ... | 1996 | 8854175 |
| reductive debromination of (alpha-bromoiso-valeryl)urea by intestinal bacteria. | the reductive debromination of the hypnotic (alpha-bromoiso-valeryl)urea to (3-methylbutyryl)urea by intestinal bacteria has been studied. the caecal contents of rats, mice, hamsters, guinea-pigs and rabbits had significant debrominating activity toward (alpha-bromoiso-valeryl)urea. the cell-free extract of intestinal bacteria from the caecal contents of rats had debrominating activity in the presence of both flavin mononucleotide (fmn) and nadh (or nadph) under anaerobic conditions. seven pure ... | 1999 | 10197422 |
| risk analysis of the thermal sterilization process. analysis of factors affecting the thermal resistance of microorganisms. | a risk analysis was applied to experimental heat resistance data. this analysis is an approach for processing experimental thermobacteriological data in order to study the variability of d and z values of target microorganisms depending on the deviations range of environmental factors, to determine the critical factors and to specify their critical tolerance. this analysis is based on sets of sensitivity functions applied to a specific case of experimental data related to the thermoresistance of ... | 1999 | 10357273 |
| identification of clostridium botulinum with api 20 a, rapid id 32 a and rapid ana ii. | three commercially available test systems for the identification of anaerobic bacteria were evaluated for the identification of 18 proteolytic group i and 69 non-proteolytic group ii clostridium botulinum, four clostridium sporogenes and 18 non-toxigenic group ii c. botulinum-like strains. all proteolytic c. botulinum strains were misidentified by the rapid id 32 a and rapid ana ii, while 14 strains and all c. sporogenes strains were identified as c. botulinum or c. sporogenes by the api 20 a. r ... | 1999 | 10397310 |
| conversion of dieldrin to aldrin by intestinal bacteria in rats. | the present study provides the evidence that dieldrin is reductively metabolized to aldrin by intestinal bacteria in rats. when dieldrin was incubated with the cecal contents of rats, aldrin, a reduced metabolite of the epoxide, was isolated from the incubation mixture. the metabolite was identified unequivocally by uv and mass spectral comparison with an authentic sample, and on the basis of its tlc and hplc behavior. the cecal contents of rats exhibited epoxide reductase activity toward dieldr ... | 1999 | 10480332 |
| evaluation of citric acid and gdl in the recovery at different ph levels of clostridium sporogenes pa 3679 spores subjected to htst treatment conditions. | spores of clostridium sporogenes pa 3679 were treated at different temperatures (121, 126, 130 and 135 degrees c) in white asparagus purée (ph 5.8) and acidified with glucono-delta-lactone (gdl) and citric acid to ph levels of 5.5, 5.0 and 4.5. afterwards, the spores were recovered in mpa3679 medium in various conditions: unacidified (ph 7.5), acidified with gdl (500 ppm) and acidified with citric acid (500 and 250 ppm) to ph levels of 6.5, 6.0 and 5.0. the results indicated that the ph levels, ... | 1996 | 8796426 |
| characterization of pentocin tv35b, a bacteriocin-like peptide isolated from lactobacillus pentosus with a fungistatic effect on candida albicans. | lactobacillus pentosus tv35b, isolated from the posterior fornix secretions of the vagina of a prenatal patient, produced a bacteriocin-like peptide (pentocin tv35b), which is inhibitory to clostridium sporogenes, cl. tyrobutyricum, lact. curvatus, lact. fermentum, lact. sake, listeria innocua, propionibacterium acidipropionici, propionibacterium sp. and candida albicans. the mechanism of activity of pentocin tv35b is bactericidal, as shown by a decrease in the viable cell numbers of lact. sake ... | 1999 | 10594714 |
| characterization of the bactericidal effects of sodium nitroprusside and other pentacyanonitrosyl complexes on the food spoilage bacterium clostridium sporogenes. | the potent bactericidal activity of sodium nitroprusside (snp; na2[fe(cn)5(no)]) towards clostridium sporogenes has been investigated. snp inhibited cell growth in the concentration range of 10 to 40 microm. concentrations above 80 microm caused irreversible loss of cell viability and cell lysis. inhibition of cell growth was similar in complex and in defined media. snp was found to be unreactive towards individual components of the defined medium, with the exception of cysteine. the chemical ch ... | 1998 | 9726859 |
| phosphine generation by mixed- and monoseptic-cultures of anaerobic bacteria. | a microbial basis for bioreductive generation of phosphine is proposed, which could account at least in part for the presence of this toxic gas in natural anaerobic environments and in sewage and landfill gases. phosphine generation under anaerobic growth conditions was dependent upon both the culture inoculum source (animal faeces) and enrichment culture conditions. phosphine was detected in headspace gases from mixed cultures under conditions promoting fermentative growth of mixed acid and but ... | 2000 | 10811253 |
| long-term bacterial profile of refrigerated ground beef made from carcass tissue, experimentally contaminated with pathogens and spoilage bacteria after hot water, alkaline, or organic acid washes. | the effects of 2% (vol/vol) lactic acid (la), 2% (vol/vol) acetic acid (aa), 12% (wt/vol) trisodium phosphate (tsp), 72 degrees c water (hw), and 32 degrees c water (w) washes on bacterial populations which were introduced onto beef carcass surfaces after wash treatments were determined up to 21 days of storage at 4 degrees c of packaged ground beef prepared from the treated and inoculated carcasses. beef carcass necks were collected from cattle immediately after harvest and subjected to the abo ... | 1998 | 9874338 |
| the involvement of coenzyme a esters in the dehydration of (r)-phenyllactate to (e)-cinnamate by clostridium sporogenes. | phenyllactate dehydratase from clostridium sporogenes grown anaerobically on l-phenylalanine catalyses the reversible syn-dehydration of (r)-phenyllactate to (e)-cinnamate. purification yielded a heterotrimeric enzyme complex (130 +/- 15 kda) composed of flda (46 kda), fldb (43 kda) and fldc (40 kda). by re-chromatography on q-sepharose, the major part of flda could be separated and identified as oxygen insensitive cinnamoyl-coa:phenyllactate coa-transferase, whereas the transferase depleted tri ... | 2000 | 10849007 |
| amplification of fluorescently labelled dna within gram-positive and acid-fast bacteria. | representative organisms from a variety of gram-positive genera were subjected to varying regimes in order to optimise the intracellular amplification of dna. the bacteria were subjected to treatments with paraformaldehyde, muramidases and mild acid hydrolysis to discover which regime made each organism permeable to the amplification reagents yet allowed retention of the fluorescein-labelled amplified products within the cell. scanning electron micrographs were used to corroborate the effectiven ... | 1999 | 10520585 |
| [on some electronic aspects of the action of certain metallic cations on the growth of aerobic bacteria (escherichia coli) and anaerobic bacteria (clostridium sporogenes)]. | | 1961 | 13891299 |
| method to sensitize bacterial spores to subsequent killing by dry heat or ultraviolet irradiation. | hydrogen peroxide and ultraviolet irradiation are known to interact synergistically for killing of bacterial spores. synergy could be demonstrated with spores of bacillus megaterium atcc19213 adsorbed to filter paper strips or glass coverslips treated first with the peroxide and then dried for as long as 48 h prior to uv irradiation. this delayed action was considered to be due to absorption of the peroxide by the spores in an active but not readily vaporized form, which could become sporicidal ... | 2000 | 11044571 |
| rapid antibiotic susceptibility testing via electrochemical measurement of ferricyanide reduction by escherichia coli and clostridium sporogenes. | electrochemical measurement of respiratory chain activity allows rapid and reliable screening for antibiotic susceptibility in microorganisms. chronoamperometry and chronocoulometry of suspensions of aerobically cultivated e. coli combined with the non-native oxidant potassium hexacyanoferrate(iii) (ferricyanide) yield signals for reoxidation of the reduction product ferrocyanide that are much smaller if the e. coli has been incubated briefly with an effective antibiotic compound. chronocoulomet ... | 2000 | 11055715 |
| bacterial spore inhibition and inactivation in foods by pressure, chemical preservatives, and mild heat. | sucrose laurates, sucrose palmitate, sucrose stearates, and monolaurin (lauricidin) were evaluated for inhibitory effects against spores of bacillus sp., clostridium sporogenes pa3679, and alicyclobacillus sp. in a model agar system. the combined treatment of sucrose laurate, high hydrostatic pressure, and mild heat was evaluated on spores of bacillus and alicyclobacillus in foods. the minimum inhibitory concentrations of the sucrose esters were higher than that of lauricidin for all spores test ... | 2000 | 11079691 |
| [18f-fdg injections produced by a solid phase 18f-fluorination (fdg microlab): effects of 18f-fdg and the components on endotoxin and sterility tests]. | effects of 18f-fdg and components of the injections on endotoxin tests (limulus tests) and sterility tests (blood culture system) were determined with 18f-fdg injections produced by a solid phase 18f-fluorination (fdg microlab, ge). 18f-fdg injections with endotoxins shortened the time for gelling (turbidimetry), compared with that of the control (saline). blood culture systems inoculated with 18f-fdg injections and microorganisms showed positive results within 72 h of incubation for every speci ... | 2001 | 11345769 |
| chlorine dioxide inactivation of cryptosporidium parvum oocysts and bacterial spore indicators. | cryptosporidium parvum, which is resistant to chlorine concentrations typically used in water treatment, is recognized as a significant waterborne pathogen. recent studies have demonstrated that chlorine dioxide is a more efficient disinfectant than free chlorine against cryptosporidium oocysts. it is not known, however, if oocysts from different suppliers are equally sensitive to chlorine dioxide. this study used both a most-probable-number-cell culture infectivity assay and in vitro excystatio ... | 2001 | 11425712 |
| reduction of stilbene oxide and styrene oxide to the corresponding alkenes by intestinal bacteria. | 1. this study provides the first evidence that stilbene oxide and styrene oxide are reductively metabolized to the corresponding alkenes by intestinal bacteria in animals. 2. when trans- or cis-stilbene oxide were incubated with the caecal contents of rat under anaerobic conditions, both trans- and cis-stilbene were isolated from the incubation mixture. styrene oxide was also reduced to styrene by rat caecal contents. 3. caecal contents of mouse, hamster and guinea pig also exhibited alkene oxid ... | 2000 | 10821165 |
| evaluation of a novel, rapid-acting, sterilizing solution at room temperature. | test data are presented for a novel chemical germicide formulation capable of sterilizing reusable medical devices in 30 minutes at 20 degrees c in an open tray. the tests conducted with this rapid-acting sterilizing solution (rss) included sporicidal, mycobactericidal, and virucidal studies performed in accordance with association of official analytical chemist international or environmental protection agency published guidelines, by using rss stressed for as long as 7 days. sporicidal assays w ... | 2000 | 11029137 |
| rejection of clostridium putrificum and conservation of clostridium botulinum and clostridium sporogenes-opinion 69. judicial commission of the international committee on systematic bacteriology. | the judicial commission rejected the name clostridium putrificum while conserving clostridium botulinum for toxigenic strains and conserving clostridium sporogenes for non-toxigenic strains. | 1999 | 10028279 |
| efficacy of oxonia active against selected spore formers. | alternatives to hydrogen peroxide are being sought for use in aseptic packaging systems because this sterilant is efficacious at temperatures higher than some of the newer packaging materials can tolerate. earlier in this century, peracetic acid was known to be bactericidal, sporicidal, and virucidal but was not widely used because of handling, toxicity, and stability problems. sanitizer suppliers have capitalized on the efficacy of hydrogen peroxide, acetic acid, and peracetic acid stabilized w ... | 1999 | 10090246 |
| effective use of nisin to control bacillus and clostridium spoilage of a pasteurized mashed potato product. | heat-resistant spore-forming bacteria such as bacillus and clostridium can survive and grow in cooked potato products. this situation represents both a public health problem and an economic problem. the natural food preservative nisin is used in heat-treated foods to prevent the growth of such bacteria. a cocktail of clostridium sporogenes and clostridium tyrobutyricum spores was inoculated into cooked mashed potatoes, which were vacuum packed, pasteurized, and incubated at 8 and 25 degrees c. t ... | 2002 | 12380742 |
| combined application of simulated reuse and quantitative carrier tests to assess high-level disinfection: experiments with an accelerated hydrogen peroxide-based formulation. | heat-sensitive medical devices require chemical disinfection between patients, and certain formulations for this purpose can be reused for several days. because dilution, evaporation, and breakdown or neutralization of active ingredients can occur during reuse, it is vital to ensure that the solution retains its broad-spectrum germicidal activity even at the end of the recommended reuse period. | 2002 | 12461509 |
| diverse effects of ascorbic acid and palmitoyl ascorbate on helicobacter pylori survival and growth. | among many antioxidants used in the food, pharmaceutical and cosmetic industries, ascorbic acid (aa) is one of the most important. aa has been suggested to decrease the risk of gastric disease (gastritis, duodenal ulcer, and carcinoma) by direct action on helicobacter pylori. however, there are limited studies on the possible role of aa and its derivatives such as palmitoyl ascorbate (pa) on the growth and survival of h. pylori. in the present study it was demonstrated in vitro that aa in the co ... | 2003 | 12892889 |
| the effect of ethylenediaminetetraacetic acid on heat resistance and recovery of clostridium sporogenes pa 3679 spores treated in htst conditions. | the effect of ethylenediaminetetraacetic acid (edta) on the heat resistance of clostridium sporogenes pa 3679 spores was studied. edta was added to heating substrates and recovery media in order to establish which stage of the heat treatment registered the greatest edta activity. the heating substrates assayed were phosphate buffer (ph 7.0) and white asparagus purée, at natural ph (5.8) and acidified with citric acid and glucono-delta-lactone (gdl) to ph 5.5, 5.0 and 4.5. recovery of survivors w ... | 1997 | 9039574 |
| [studies on degradation of keto acids by clostridium sporogenes extracts]. | | 1954 | 13141467 |
| effects of carbohydrates, proteins, and bacterial cells in the heating media on the heat resistance of clostridium sporogenes. | | 1954 | 13201532 |
| use of the dropping mercury electrode for measuring oxygen changes in treponema and clostridium sporogenes cultures. | | 1956 | 13292937 |
| biotin and arginine replacements in the nutrition of clostridium sporogenes. | | 1950 | 15436459 |
| energy supply and enzyme activity in strict anaerobes, studies on peptidase activity in clostridium sporogenes. | | 1957 | 13412734 |
| studies of the fine structure of microorganisms. ii. electron microscopic studies on sporulation of clostridium sporogenes. | | 1958 | 13549367 |
| [experimental inoculation of the chick embryo with clostridium sporogenes]. | | 1962 | 13884991 |
| [research on the odors emitted by anaerobic bacteria. clostridium sporogenes, marine and soil strains. comparison of their lipidolytic potency]. | | 1962 | 13968287 |
| characterization of culturable anaerobic bacteria from the forestomach of an eastern grey kangaroo, macropus giganteus. | to determine the culturable biodiversity of anaerobic bacteria isolated from the forestomach contents of an eastern grey kangaroo, macropus giganteus, using phenotypic characterization and 16s rdna sequence analysis. | 2005 | 16162139 |
| [rare case of pyo-gaseous abscess of the liver caused by clostridium sporogenes-bacterium pyocyaneum]. | | 1963 | 13972701 |
| sarcosine reductase of tissierella creatinophila: purification and characterization of its components. | sarcosine reductase is the only reductase system present in tissierella creatinophila when grown on creatinine plus formate. the acetyl-phosphate-forming component protein c was purified to homogeneity. sds-page of the purified protein revealed two protein bands with apparent mol. masses of 62 and 50 kda. the n-terminal amino acid sequence of the two subunits was determined. antibodies raised against each of the subunits of protein c from eubacterium acidaminophilum cross-reacted with the corres ... | 1998 | 9799288 |
| the spore antigens of clostridium sporogenes, cl. bifermentans and cl. sordellii. | | 1963 | 13998504 |
| effect of ph on the heat resistance of spores comparison of two models. | all published models describing the effect of ph on the heat resistance of spores can be regarded either as a linear first degree equation or a linear second degree equation. this work aimed to compare both models from three sets of published data for, clostridium sporogenes and bacillus stearothermophilus, respectively. the relative quality of fit of each model with respect to the other depends on the species, the strain and the heating temperature. parameter estimation was more reliable for th ... | 2001 | 11205953 |
| radiobiologic experiments in discoverer satellite xviii. physiologic changes observed in clostridium sporogenes. | | 1962 | 14025478 |
| combined effects of ultrahigh vacuum and temperature on the viability of some spores and soil organisms. | considerably fewer spores of bacillus stearothermophilus, b. megaterium, and clostridium sporogenes were recovered than were spores of b. subtilis var. niger and aspergillus niger after 4 to 5 days at 53 and 60 c in ultrahigh vacuum. there were no significant differences in the recoveries of these five organisms at 25 c and atmospheric pressure, and after exposure to 25 and -190 c in vacuum. at 60 c, a far greater decrease in viability was demonstrated for b. stearothermophilus, b. megaterium, a ... | 1963 | 14025505 |