Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| cloning, nucleotide sequence and expression of a hemolysin gene of clostridium septicum. | a genomic library of clostridium septicum nctc547 strain was made in escherichia coli by means of lambda gt10. the dna insert of a hemolysin-positive (hly+) lambda-clone was transferred into puc19. the resulting plasmid, pcs21, confers a hly+ phenotype on e. coli. crude lysates of e. coli (pcs21) possessed a strong lytic activity on human erythrocytes and also a lethal effect on mice, characteristic of an alpha toxin. nucleotide sequence analysis revealed that the insert dna (5.2 kb) in pcs21 in ... | 1994 | 8200504 |
| invasive clostridium septicum infection in association with colorectal carcinoma. | 1994 | 7922895 | |
| clostridium septicum gas gangrene of the gluteus maximus and an ascending colon malignant tumor. a case report. | clostridial myonecrosis is a complication associated with contaminated traumatized wounds. presented is the case of an elderly female with pain in her right hip, radiographic evidence of gas in the soft tissues, and no history of trauma. evaluation of this patient revealed clostridial myonecrosis. culture results identified the organism as clostridium septicum. due to the association between clostridium septicum and occult malignancies, colonoscopic evaluation was performed. a colonic lesion was ... | 1994 | 7955680 |
| clostridium septicum syndrome. | 1994 | 7978581 | |
| spontaneous clostridium septicum myonecrosis in congenital neutropaenia. | spontaneous clostridium septicum myonecrosis is an uncommon disorder that has been described in association with malignancy, immunosuppression and neutropaenia. typical clostridial myonecrosis develops without a visible portal of entry and mortality is high. the pathogenesis is not completely understood but the clostridia may gain access to the circulation via areas of ileo-caecal ulceration secondary to enterocolitis, antibiotics or neoplasms. a 5 year old boy with congenital neutropaenia prese ... | 1994 | 8048901 |
| [atraumatic clostridium septicum infection in acute myeloid leukemia]. | 1994 | 8072458 | |
| differentiation of hl-60 cells is promoted by h-toxin of clostridium septicum. | h-toxin of clostridium septicum potentiated dimethyl sulfoxide (dmso)-induced differentiation of human promyelocytic leukemia hl-60 cells which were monitored by nuclear morphology and production of oxidative radicals. but, h-toxin did not induce differentiation of hl-60 cells in the absence of dmso. these phenomena were not observed by staphylococcal leukocidin, a cytotoxin affecting to hl-60 cells. in hl-60 cells, adp-ribosylation of 118, 93, 75 and 58 kda membrane proteins was observed, but t ... | 1994 | 8082787 |
| clinical courses of seven survivors of clostridium septicum infection and their immunologic responses to alpha toxin. | clostridium septicum bacteremia typically portends a fulminant disease associated with high mortality. we describe the clinical courses of seven survivors of c. septicum infection and their antibody responses to the alpha toxin produced by c. septicum. three patients had clinical syndromes ranging from uncomplicated bacteremia to early typhlitis, and three patients had syndromes ranging from abscess to myonecrosis and septic shock. in addition, an aids patient who developed septic shock and who ... | 1994 | 7803645 |
| gene structure of the 'large' sialidase isoenzyme from clostridium perfringens a99 and its relationship with other clostridial nanh proteins. | clostridium perfringens possesses two sialidase isoenzymes of different molecular weight. almost 90% of the gene encoding the 'large' form was found on a 3.1 kb chromosomal fragment (sau3ai) of strain a99 by hybridization with probes developed from the n-terminal protein sequence and from commonly conserved sialidase motifs ('asp-boxes'), whereas the remaining 3'-terminal part was detected on a 2.1 kb fragment (hind iii) of chromosomal dna. after combination of both fragments, the resulting e. c ... | 1994 | 7804004 |
| clostridium septicum abscess in hepatic metastases: successful medical management. | clostridium septicum bacteremia is frequently associated with hematologic and colonic malignancies and neutropenia. it frequently produces 'metastatic' gangrene with excessive mortality. standard therapy usually includes surgical debridement and antibiotics. we present a patient with metastatic breast cancer treated with high-dose chemotherapy and bone marrow transplantation. she was treated successfully with antibiotics alone despite developing cl. septicum bacteremia and gas in hepatic metasta ... | 1994 | 7517261 |
| diversity in molecular mass of the common edta-soluble antigens of clostridium chauvoei and clostridium septicum. | common edta-soluble antigens of clostridium chauvoei and c. septicum were examined by indirect-immunofluorescence (ifa) and immunoblot analysis. the monoclonal antibodies (mabs) specific for the 35 kda antigen of c. chauvoei strain atcc 10092 were used. these mabs reacted with all 11 strains, 6 of c. chauvoei and 5 of c. septicum, in ifa. in immunoblot analysis with the mabs, the bands at molecular mass of 35 kda were found in all c. chauvoei strains, while the bands at 36 kda were found in 4 of ... | 1994 | 7518890 |
| clostridium septicum septicaemia in a patient with leukaemia. | clostridium septicum infection has been shown to have a strikingly high association with either bowel or blood malignancies. the infection may be fatal if unrecognised. we report a case of c. septicum bacteremia in a man diagnosed with acute myeloid leukaemia. | 1994 | 7939827 |
| spontaneous occurrence of gangrene due to clostridium septicum in a patient with advanced endometrial carcinoma. | we report the first known case of spontaneous, atraumatic clostridium septicum gangrene occurring in a patient with recurrent endometrial adenocarcinoma. | 1994 | 18475364 |
| pasteur, oxygen and the anaerobes revisited. | as a biochemist, louis pasteur focused on fermentation, demonstrating that it was a vital process. in 1860, he discovered anaerobic life and the strict anaerobes, particularly those responsible for butyric fermentation. then, in spite of his lack of medical background, pasteur turned to investigating the role of bacteria in human and animal diseases. in 1877, pasteur and joubert described for the first time a pathogenic anaerobe, the 'septic vibrio' (now clostridium septicum). not only was the b ... | 1995 | 16887501 |
| clostridial disease of the gut. | clostridia are an important cause of morbidity and mortality in humans and animals. some of the most common clostridial infections are those of the gut. the primary infections in humans are clostridium perfringens food poisoning and clostridium difficile-mediated antibiotic-associated diarrhea and colitis. less common but important infections include non-food poisoning c. perfringens nosocomial diarrhea and c. perfringens type c necrotizing jejunitis (pig-bel). c. perfringens is also the dominan ... | 1995 | 7548565 |
| clostridium septicum bacteremia in a patient with large granular lymphocyte leukemia. | this is the first case report of clostridium septicum septicemia in a patient with large granular lymphocyte leukemia. c. septicum infection is highly associated with malignancy and causes a rapidly fatal enterocolitis among patients who are profoundly neutropenic. the need for early recognition and combination of early antibiotic therapy and necessary surgical intervention may help to alter the fulminating nature of c. septicum infection. | 1995 | 7552816 |
| clostridium septicum bacteremia associated with aortic graft infection. | after emergency repair of a ruptured abdominal aortic aneurysm associated with an aortocaval fistula, clostridium septicum sepsis prompted evaluation for colon cancer. adenocarcinoma of the right colon ultimately required hemicolectomy, after which the patient had development of recurrent c. septicum bacteremia. computed tomography scanning demonstrated a large fluid collection surrounding the aortic graft, and percutaneous drainage documented recurrent c. septicum. initial axillobifemoral bypas ... | 1995 | 7563411 |
| malignancy, mortality, and medicosurgical management of clostridium septicum infection. | necrotizing clostridium septicum infections (csi) have a strong association with malignancy or immunosuppression. to clarify this relationship and determine how it impacted mortality, the experience with csi at a single institution was reviewed. | 1995 | 7570310 |
| [gas gangrene as presentation of colonic carcinoma in familial adenomatous polyposis]. | one case of gas gangrene by clostridium septicum associated to colon cancer is herein presented. spontaneous gas gangrene is often associated with neoplasms among which hematologic and colon neoplasms are the most frequent. the present case has also peculiarity that the colon cancer had developed from a familiar adenomatous polyposis syndrome. | 1995 | 7584783 |
| peritonitis due to clostridium septicum in a capd patient. | 1995 | 7734578 | |
| clostridium septicum primary myonecrosis related to cecal neoplasia. | 1995 | 7775706 | |
| [infection by clostridium septicum as a presenting form of colonic neoplasia]. | 1995 | 7779902 | |
| brain abscesses caused by clostridium septicum as a complication of hemolytic-uremic syndrome. | 1995 | 7715999 | |
| purulent pericarditis due to clostridium septicum associated with carcinoma of the colon. | 1995 | 7727661 | |
| myonecrosis due to clostridium septicum in a patient with unexplained neutropenia: successful treatment with granulocyte colony-stimulating factor. | we report a case of sepsis due to clostridium septicum successfully treated with granulocyte colony-stimulating factor (gcsf). this case prompted our review of clostridial sepsis and considerations regarding the use of gcsf in cases of drug-induced neutropenia. | 1995 | 7541162 |
| the primary structure of clostridium septicum alpha-toxin exhibits similarity with that of aeromonas hydrophila aerolysin. | the gene for clostridium septicum alpha-toxin was cloned and expressed in escherichia coli from c. septicum bx96. the toxin was determined to be 443 amino acids in length, with a 31-residue signal peptide that was removed from the toxin during secretion. no extended hydrophobic regions were observed in the mature toxin sequence. expression of alpha-toxin in e. coli bl21 resulted in the production of atpro, which was identical to native toxin from c. septicum with respect to activity and activati ... | 1995 | 7806374 |
| double clostridial mycotic aneurysms of the aorta. | mycotic aortic aneurysm continues to present challenging and difficult management issues with a significant morbidity and mortality. the offending organism in the etiology of this aneurysm can be variable and unusual. the first report of two mycotic aortic aneurysms caused by clostridium septicum in the same patient is described here. presentation and management as well as conditions commonly associated with clostridium septicum infection and a review of all clostridial mycotic aortic aneurysms ... | 1995 | 8745194 |
| gas composition in clostridium septicum gas gangrene. | clostridial gas gangrene (myonecrosis) is a rare but catastrophic condition that usually occurs in patients with underlying diseases. this paper reports a fatal case of spontaneous clostridial gas gangrene in a 60-year-old female diabetic patient. the composition of gas samples from the patient's damaged muscle was analyzed. the results showed 5.9% hydrogen, 3.4% carbon dioxide, 74.5% nitrogen and 16.1% oxygen. this gas composition supports the belief that such gas production occurs via glucose ... | 1995 | 8541740 |
| [gas gangrene as a manifestation of endogenous clostridium septicum infection]. | endogenous, nontraumatic clostridial myonecrosis has a frequent association with colon carcinoma, leukemia, diabetes mellitus, and drug-induced immunosuppression. we present two cases of clostridium septicum myonecrosis. an 18-year-old girl developed severe abdominal pain on day 7 after hospitalization for cytostatic treatment of acute lymphoblastic leukemia. blood cultures yielded clostridium septicum and histopathological exam of muscle tissue showed extended myonecrosis. eventually the patien ... | 1995 | 8582739 |
| primary clostridium septicum aortitis: a rare cause of necrotizing suprarenal aortic infection. a case report and review of the literature. | a 74-year old woman sought medical attention for general symptoms of nausea, vomiting, and back pain. a computed tomographic scan showed gas in the wall of the descending thoracic and suprarenal aortas. emergency thoracoabdominal exploration revealed a necrotizing infection of the thoracic aorta extending to the origin of the celiac axis. after surgery clostridium septicum was identified in tissue culture. surgical management consisted of in-situ graft replacement of the thoracoabdominal aorta. ... | 1996 | 8627911 |
| cloning and expression of sialidase l, a neuacalpha2-->3gal-specific sialidase from the leech, macrobdella decora. | sialidase l is a neuacalpha2-->3gal linkage-specific sialidase that releases 2,7-anhydro-neuac instead of neuac from sialoglycoconjugates (chou, m.-y., li, s.-c., kiso, m., hasegawa, a., and li, y.-t.(1994) j. biol. chem. 269, 18821-18826). a 2. 5-kilobase cdna of sialidase l was cloned by a combination of methods based on polymerase chain reactions. the composite cdna sequence reveals an open reading frame coding for 762 amino acids, including a putative 28-residue signal peptide at the n termi ... | 1996 | 8702601 |
| periodic recurrence of gangrenous dermatitis associated with clostridium septicum in a broiler chicken operation. | 1996 | 8744754 | |
| cloning and characterization of nanb, a second streptococcus pneumoniae neuraminidase gene, and purification of the nanb enzyme from recombinant escherichia coli. | streptococcus pneumoniae is believed to produce more than one form of neuraminidase, but there has been uncertainty as to whether this is due to posttranslational modification of a single gene product or the existence of more than one neuraminidase-encoding gene. only one stable pneumococcal neuraminidase gene (designated nana) has been described. in the present study, we isolated and characterized a second neuraminidase gene (designated nanb), which is located close to nana on the pneumococcal ... | 1996 | 8759848 |
| phase i trial of high-dose infusional hydroxyurea, high-dose infusional 5-fluorouracil and recombinant interferon-alpha-2a in patients with advanced malignancies. | the ribonucleotide reductase inhibitor, hydroxyurea (hu), augments the cytotoxic effects of 5-fluorouracil (5fu) in vitro; both drugs are synergistic with interferon-alpha (ifn) in vitro. the aim of this phase i study was to determine the maximal duration of hu, 4.3 g/m2, administered as a parenteral infusion in combination with 5fu, 2.6 g/m2 administered over 24 hrs each week, + ifn, 9 mu, subcutaneously three times per week. there were 26 patients enrolled and evaluable. this included 14 patie ... | 1996 | 8824349 |
| phylogenetic positions of clostridium chauvoei and clostridium septicum based on 16s rrna gene sequences. | the sequences of the 16s rrna genes (rrs genes) of clostridium chauvoei, the causative agent of blackleg in cattle, and the phenotypically related organism clostridium septicum were determined. after amplification of 1,507-bp pcr fragments from the corresponding rrs genes, the sequences were determined in a single round of sequencing by using conserved region primers. a sequence similarity analysis of the sequences revealed the close phylogenetic relationship of c. chauvoei and c. septicum in cl ... | 1996 | 8863454 |
| mycotic aneurysm of the thoracic aorta caused by clostridium septicum. | we describe a case-of a 78-year-old man who presented with a mycotic aneurysm of the thoracic aorta caused by clostridium septicum and underwent successful resection. there are only 3 cases of mycotic aneurysms caused by clostridium septicum reported in the literature. clostridium septicum infections have been shown to have a high association with gastrointestinal and hematologic malignancies. all patients with clostridium septicum infections, therefore, require a search for gastrointestinal les ... | 1996 | 8957398 |
| bovine clostridial infections in zambia (1985-1994). | retrospective surveillance study of clostridial infections of cattle in zambia, for the period 1985 to 1994, showed that out of the 318 cases observed, 62.8% and 24.2% were from western and southern provinces, respectively. of the 6 clostridia species identified, clostridium septicum (38.1%) followed by c. chauvoei (36.2%) and c. perfringens (13.2%) were dominant. although the highest incidence for clostridial infections was in 1989 (75 cases) and 1990 (77 cases), the number of c. perfringens ca ... | 1996 | 8997878 |
| [bilateral lower leg pain as a sign of clostridium septicum infection]. | 1996 | 10592641 | |
| [gas gangrene of ischemic myocardial tissue caused by clostridium septicum]. | a 86-year-old woman hospitalized for analysis of persistent abdominal discomfort died, apparently of myocardial infarction, shortly after admission. autopsy revealed extensive myocarditis caused by infection with clostridium septicum. as a portal of entry a carcinoma of the colon was found. the association of colon carcinoma and bacteraemia with c. septicum is well established while colon carcinoma can lead to cardiac hypoxia through blood loss and anaemia. however, myocarditis caused by this or ... | 1996 | 9053745 |
| clostridium septicum sepsis and neutropenic enterocolitis in a patient treated with intensive chemotherapy for acute myeloid leukemia. | we report a case of neutropenic enterocolitis complicated by clostridium septicum sepsis following intensive chemotherapy to induce remission of acute myeloid leukemia. with the trend towards more intensive chemotherapy, particularly using regimens that induce gastrointestinal toxicity, it is important to recognize the circumstances under which sepsis due to c. septicum is likely to occur, so that appropriate treatment can be instituted promptly. | 1997 | 9111429 |
| mycotic thoracic aneurysm due to clostridium septicum and occult adenocarcinoma of the cecum. | 1997 | 9142770 | |
| central nervous system infection due to clostridium septicum: a case report and review of the literature. | a patient with end stage breast cancer was admitted to hospital due to fever, chills, multiply eroded discharging wounds, and sudden onset of left hemiparesis. clostridium septicum bacteremia and brain abscess were diagnosed. the patient was treated successfully with intravenous penicillin and clindamycin and stereotactic aspiration of the abscess. eleven cases of c. septicum central nervous system infection are reviewed. they showed an extremely fulminant course and high fatality. nevertheless, ... | 1997 | 9181386 |
| nontraumatic gas gangrene. | a 77-year-old man with a history significant only for coronary artery disease presented to the ed with left-arm pain, shortness of breath, nausea, and diaphoresis. six hours after the patient's admission to the hospital for presumed unstable angina, fever and left arm swelling, associated with crepitus and violaceous bullae, developed. the patient was taken to the operating room, where he was found to have extensive myonecrosis requiring forequarter amputation of the left arm. nontraumatic clost ... | 1997 | 9209235 |
| spontaneous gas gangrene: an unusual complication of colonic carcinoma. | clostridium septicum myonecrosis is an acutely painful and rapidly fatal infection occurring in the absence of trauma. urgent surgery is essential both to maximize survival and to provide effective pain control. the majority of patients who develop this infection have an underlying malignancy and clinicians involved in the care of cancer patients should be aware of this condition. we present a case report and summarize the literature to date. | 1997 | 9269553 |
| clostridium septicum infection in children with cyclic neutropenia. | atraumatic clostridium septicum infection is rare in infancy and childhood and is associated with a high mortality rate. although in adults it has been reported to occur mainly in patients with gastrointestinal malignancy, pediatric cases were always associated with neutropenia. about 70% of the cases were described in children with neutropenia caused by chemotherapy and 30% were found in children with cyclic neutropenia. no case was described in children with other forms of congenital severe ne ... | 1997 | 9290625 |
| isolated meningeal vasculopathy associated with clostridium septicum infection. | a 28-year-old patient with acute lymphoblastic leukemia and neutropenia developed necrotizing enterocolitis and clostridium septicum bacteremia, followed by rhabdomyolysis, skin rash, and acute neurologic changes. numerous cortical leptomeningeal enhancements were present on head mri. meningeal and brain biopsy showed segmental, full-thickness lysis of smooth muscle cells of medium-sized meningeal vessels with overall preservation of the structure of the vessel wall. | 1997 | 9008531 |
| generation of a membrane-bound, oligomerized pre-pore complex is necessary for pore formation by clostridium septicum alpha toxin. | low-temperature inhibition of the cytolytic activity of alpha toxin has facilitated the identification of an important step in the cytolytic mechanism of this toxin. when alpha toxin-dependent haemolysis was measured on erythrocytes at various temperatures it was clear that at temperatures < or = 15 degrees c the haemolysis rate was significantly inhibited with little or no haemolysis occurring at 4 degrees c. alpha toxin appeared to bind to and oligomerize on erythrocyte membranes with similar ... | 1997 | 9044288 |
| a coincidence of disastrous accidents: crohn's disease, agranulocytosis, and clostridium septicum infection. | 1997 | 9314329 | |
| clostridium septicum alpha-toxin is proteolytically activated by furin. | clostridium septicum alpha-toxin is secreted as an inactive 46,450-da protoxin. the protoxin is activated by proteolytic cleavage near the c terminus, which eventually causes the release of a 45-amino-acid fragment. proteoytic activation and loss of the propeptide allow alpha-toxin to oligomerize and form pores on the plasma membrane, which results in colloidal-osmotic lysis. activation may be accomplished in vitro by cleavage with trypsin at arg367 (j. ballard, y. sokolov, w. l. yuan, b. l. kag ... | 1997 | 9317018 |
| detection of clostridium septicum hemolysin gene by polymerase chain reaction. | a polymerase chain reaction (pcr) was developed for the detection of the hemolysin (alpha toxin) gene of clostridium septicum. the pcr primers were designed from the sequence of the hemolysin gene and synthesized. a dna fragment of 270 bp was amplified from 10 strains of c. septicum, but was not from strains of c. chauvoei, c. perfringens, c. novyi, or c. haemolyticum. when the pcr product was digested with sau3ai, two dna fragments of the expected 148 bp and 122 bp were recognized. the lowest d ... | 1997 | 9342717 |
| another cause of necrotizing fasciitis? | necrotizing fasciitis is a rare condition, but has a devastating clinical course if it is not diagnosed early and treated aggressively. there are usually a number of organisms implicated in its pathogenesis, but in this case report a patient is presented in whom the only organism isolated from both tissue and blood cultures was clostridium septicum. this organism almost always causes a myonecrosis, but in this case only the superficial and deep fascial layers were affected, sparing the underlyin ... | 1997 | 9354354 |
| identification of clostridium chauvoei in cultures and clinical material from blackleg using pcr. | an identification system for clostridium chauvoei, using pcr amplification of the 16s rrna gene (rrs) with specific oligonucleotide primers and subsequent restriction digestion of the amplification product is described. the specific oligonucleotide primers were designed based on the rrs gene sequences of c. chauvoei by comparing it to the dna sequences of the rrs genes of its most closely related species clostridium septicum and clostridium carnis. a subsequent restriction digestion of the 960 b ... | 1997 | 9355263 |
| aortic dissection due to clostridium septicum infection. | 1997 | 9166278 | |
| [gas gangrene due to clostridium septicum in a patient with an occult colonic neoplasm. a case report and review of the literature]. | a case of gaseous gangrene by clostridium septicum associated with colorectal cancer is presented. the patient evolved rapidly towards septic shock and death. autopsy showed occult neoplasm and pelvic and retroperitoneal myonecrosis. an exceptional finding was that of myocarditis in which thick gram-positive bacilli were identified. a review of the literature was carried out regarding the pathogenesis and clinical manifestations of this disease. the association of colonic neoplasm and clostridiu ... | 1997 | 9072204 |
| the propeptide of clostridium septicum alpha toxin functions as an intramolecular chaperone and is a potent inhibitor of alpha toxin-dependent cytolysis. | clostridium septicum alpha toxin is activated by a proteolytic cleavage at arg-398 in its carboxy terminus, which yields a 41.3-kda cytolytically active toxin and a 5.1-kda propeptide. studies were performed to determine when the propeptide dissociated from the toxin after proteolytic activation of the protoxin (at(pro)) and to demonstrate the chaperone activity of the propeptide. the propeptide was found to remain associated with the toxin after activation with trypsin (at(act)) when analysed b ... | 1997 | 9302006 |
| effect of temperature and glucose concentration on dnase production by clostridium septicum. | 1997 | 9310663 | |
| antibody responses to clostridial infection in humans. | serum antibody responses to the major toxins produced by clostridium difficile, clostridium perfringens, clostridium septicum, clostridium tetani, and clostridium botulinum have been documented following infection. effective toxoid vaccines for tetanus and enteritis necroticans due to c. perfringens type c demonstrate the potential of antitoxin responses. although individual serum and mucosal antibody responses to c. difficile enterotoxin (toxin a) vary, one-third of patients with c. difficile d ... | 1997 | 9310668 |
| alpha-toxin production by clostridium septicum at different culture conditions. | clostridium septicum is responsible for a number of diseases, the most serious of which are a frequently fatal non-traumatic gas gangrene in man and braxy malignant oedema and blackquarter in farm animals. immunity to these diseases is mediated mainly by antitoxin raised against c. septicum alpha toxin, which has hemolytic, lethal and necrotizing activities. clostridium septicum produces lethal antigen only in low titre and in a variable way. in addition, the immunogenicity of native toxic filtr ... | 1997 | 16887590 |
| [development of a cytotoxin inhibition test for the detection of serum antibodies against the alpha-toxin of clostridium septicum] | clostridium (c.) septicum, a spore-forming bacterium of the soil, is the classical causative agent of the malignant oedema, a fatal infection. as immunogenic compound, vaccines contain the toxoidized form of the soluble alpha-toxin, a lethal exoprotein. a cytotoxin inhibition test based upon cell culture for the detection of toxin neutralizing antibodies was developed as an alternative to the neutralisation test in mice, which has to be done as measure of quality control according to dab 10. ser ... | 1998 | 11178543 |
| clostridium septicum infection and hemolytic uremic syndrome. | five cases of clostridium septicum infection secondary to escherichia coli o157-induced hemolytic uremic syndrome have been reported. we report on three cases (one of which is included in the above five) of dual cl. septicum and e. coil infection; all three patients were exposed to farm animals. a common zoonotic source for cl. septicum and e. coli o157 infections should be considered. patients with hemolytic uremic syndrome should be treated aggressively and monitored closely for cl. septicum s ... | 1998 | 9621207 |
| clostridium septicum myonecrosis presenting as a parapharyngeal abscess in a patient with aplastic anemia. | the first reported case of clostridium septicum myonecrosis in an adult with aplastic anemia is described. the patient presented with sepsis, a parapharyngeal abscess that necessitated emergent intubation, and severe intravascular hemolysis attributed to clostridial alpha-toxin production. despite prompt recognition and treatment, the patient died of his infection. c. septicum myonecrosis should be considered in any immunocompromised patient with sepsis, especially when accompanied by evidence o ... | 1998 | 9795792 |
| clostridium septicum osteomyelitis: case report and review. | 1998 | 9802642 | |
| clostridium septicum endocarditis complicated by aortic-ring abscess and aortitis. | 1998 | 9502477 | |
| aerolysin--a paradigm for membrane insertion of beta-sheet protein toxins? | the determination of the crystal structure of the bacterial protein proaerolysin provided the first view of a pore-forming toxin constructed mainly from beta-sheet. the structure that was obtained and subsequent crystallographic and biochemical studies have together allowed us to explain how the toxin is transformed from a water-soluble dimer to a heptameric transmembrane pore. recent discoveries of structural similarities between aerolysin and other toxins suggest that the structure/function st ... | 1998 | 9615432 |
| enzymatic and molecular properties of the clostridium tertium sialidase. | clostridium tertium metabolizes sialoglycoconjugates via a secreted sialidase [ec 3.2.1.18] and an intracellular acylneuraminate pyruvate lyase [ec 4.1.3.3]. the sialidase was enriched 1,900-fold from the culture medium with a specific activity of 0.7 u per mg protein. it exhibits a temperature optimum of 50 degreesc and tolerates mercury ions at relatively high concentrations (50% inhibition at 5.2 mm hg2+). the sialidase gene was detected on two restriction fragments (hincii, hindiii) of chrom ... | 1998 | 9832614 |
| cellulitis due to escherichia coli in three immunocompromised subjects. | in adults, cellulitis is usually caused by group a streptococci and staphylococcus aureus. however, in patients with underlying disease, it may be caused by other organisms, such as acinetobacter, clostridium septicum, enterobacter, haemophilus influenzae, proteus mirabilis or escherichia coli. we report three cases of cellulitis of the lower legs where e. coli was the causative bacterial organism. it is important to suspect e. coli as a causative organism if blistering cellulitis occurs, especi ... | 1998 | 9892960 |
| [enterocolitis in a neutropenic patient and bacteremia caused by clostridium septicum]. | 1998 | 9835162 | |
| exogenously acquired clostridium septicum gas gangrene--a case report. | we report on a 72-year-old woman suffering from gas gangrene of the right leg, which developed 3 days after getting injured while gardening. due to rapid microbiological diagnosis-direct microscopical examination of muscle biopsies revealed numerous grampositive rods-, immediately performed amputation of the leg and antibiotic treatment with penicillin and metronidazole the patient survived. from the muscle biopsies clostridium septicum, which is found ubiquitously, was isolated in pure culture. | 1998 | 9887679 |
| [cell cultures as a system for distinguishing between strains ofclostridium chauvoei and clostridium septicum isolated in northeastern mexico]. | clostridium chauvoei and c. septicum have similar characteristics as far as results from biochemical methods and gas chromatography (gc) are concerned. a total of 267 samples collected from sick or dead animals in the fields from northeast mexico, were bacteriologically analysed and differentiated by the gc technique. from these strains, 16 belong to the group of c. chauvoei/c. septicum. studies on the effect of toxin on cell cultures of the lines ebl, 3t3, bhk21-bsr/pk5/88, cho-k1 and mdck were ... | 1999 | 10932771 |
| clostridium septicum myonecrosis and ovarian cancer: a case report and review of literature. | a case report of lethal distant myonecrosis with gas gangrene is presented. cultures from blood and suppuration of our patient revealed clostridium septicum. at obduction the patient appeared to have a necrotic metastasis of a known ovarian carcinoma in the cecum wall. predisposing conditions for this type of infection are hematologic malignancies, colon carcinoma, neutropenia, diabetes mellitus, and disruption of the bowel mucosa. clostridium septicum is highly associated with the presence of a ... | 1999 | 9889043 |
| expression and properties of an aerolysin--clostridium septicum alpha toxin hybrid protein. | aerolysin is a bilobal channel-forming toxin secreted by aeromonas hydrophila. the alpha toxin produced by clostridium septicum is homologous to the large lobe of aerolysin. however, it does not contain a region corresponding to the small lobe of the aeromonas toxin, leading us to ask what the function of the small lobe is. we fused the small lobe of aerolysin to alpha toxin, producing a hybrid protein that should structurally resemble aerolysin. unlike aerolysin, the hybrid was not secreted whe ... | 1999 | 10048023 |
| clostridium septicum osteomyelitis with intramedullary gas on ct and mr imaging. | 1999 | 10227549 | |
| detection of neutralizing antibodies against alpha-toxin of different clostridium septicum strains in cell culture. | clostridium septicum, a ubiquitious organism, is the pathogen which causes the classical malignant edema after injuries. because of its strong cytotoxic alpha-toxin, infections are often lethal. to prevent losses in animals, vaccination with alpha-toxoid vaccines is carried out. quality control of the vaccines is done by a neutralization test in mice. a cytotoxin test and as an alternative method to detect neutralizing antibodies, a cytotoxin inhibition test was standardized. in the studies, alp ... | 1999 | 10397322 |
| nontraumatic clostridial myonecrosis. | we describe three cases of nontraumatic clostridial myonecrosis seen at the victorian institute of forensic medicine. nontraumatic clostridial myonecrosis is an uncommon and often fatal condition that requires immediate institution of appropriate medical and surgical therapy. it is most commonly caused by clostridium perfringens and clostridium septicum and is associated with gastrointestinal and hematologic malignancies, diabetes mellitus, and peripheral vascular disease. the clinical features ... | 1999 | 10414657 |
| clostridium septicum alpha toxin uses glycosylphosphatidylinositol-anchored protein receptors. | the alpha toxin produced by clostridium septicum is a channel-forming protein that is an important contributor to the virulence of the organism. chinese hamster ovary (cho) cells are sensitive to low concentrations of the toxin, indicating that they contain toxin receptors. using retroviral mutagenesis, a mutant cho line (bag15) was generated that is resistant to alpha toxin. facs analysis showed that the mutant cells have lost the ability to bind the toxin, indicating that they lack an alpha to ... | 1999 | 10480947 |
| case report: clostridium septicum infection presenting as liver abscess in a case of choriocarcinoma with liver metastasis. | clostridium septicum is an anaerobic, gram-positive bacillus. infection with this organism has a known association with malignant diseases, especially colon and haematological cancers. clostridium septicum is rarely found to be a pathogen of liver abscess. herein, we report the case of a female choriocarcinoma patient with liver metastasis in which c. septicum infection presented as a gas-forming liver abscess. this case and previous reports indicate that once c. septicum is identified as a path ... | 1999 | 10634163 |
| clostridium septicum septicaemia with myonecrosis. | a case of fatal spontaneous gas gangrene due to clostridium septicum septicaemia associated with an occult rectal malignancy is presented. this condition has a rapid progression and a high mortality even with prompt treatment. it is important that the radiologist considers this diagnosis in an appropriate clinical setting to allow rapid instigation of appropriate therapy. | 1999 | 10901913 |
| clostridium septicum bacteremia in the pediatric population. | 2000 | 11079000 | |
| [gas gangrene in clostridium septicum sepsis]. | 2000 | 11092176 | |
| clostridium septicum: a malign pathogen. | 2000 | 11120626 | |
| presence of phospholipase-d (dly) gene coding for damselysin production is not a pre-requisite for pathogenicity in photobacterium damselae subsp. damselae. | the presence of the phospholipase-d (dly) gene as pre-requisite for virulence of photobacterium damselae subsp. damselae for poikilotherm and homoiotherm animals was investigated in a total of 17 strains isolated from fish, shellfish, mammals and seawater. with this aim, we developed two pcr protocols. a simple pcr using primers flanking the almost complete dly gene, and a multiplex-pcr using two sets of primers directed to internal fragments of the dly and 16s rrna genes. only six of the 17 ph. ... | 2000 | 10644497 |
| the role of clostridium septicum in paraneoplastic sepsis. | clostridium septicum infections are rare but often associated with serious if not fatal outcomes. clostridium septicum infection does not appear to be associated with a single specific defect in cellular or humoral immunity. it has been associated with multiple medical problems, including but not limited to leukemia, malignancy of the bowel, other solid tumors, cyclic neutropenia with enterocolitis, diabetes mellitus, and severe arteriosclerosis. most cases of c septicum are associated with mali ... | 2000 | 10705386 |
| fulminant clostridium septicum infection of hepatic metastases presenting as pneumoperitoneum. | 2000 | 10749230 | |
| sepsis due to clostridium septicum: case report. | 2000 | 10786915 | |
| cns infection with clostridium septicum. | we present an unusual case of clostridium septicum brain infection in a 72-yr-old woman who had no underlying malignant disease. the infection spread from a localized sit to the cns causing gas formation. the patient died rapidly. | 2000 | 10879607 |
| clostridium septicum infection of the aorta. | 2001 | 11529239 | |
| clinical features of clostridial bacteremia: a review from a rural area. | blood samples, which were obtained from patients who lived in a rural area with approximately 500 acute-care hospital beds, were cultured from 1990 through 1997. we retrospectively reviewed the blood cultures that yielded clostridium species (74 [0.12%] of 63,296 cultures). these were obtained from 46 different hospitalized patients (incidents per hospital, 0.03%). the source of the clostridium species was a gastrointestinal site in 24 patients (52.2%). the most frequently identified clostridium ... | 2001 | 11438901 |
| clostridium bacteremia: emphasis on the poor prognosis in cirrhotic patients. | bacteremic episodes caused by anaerobes are unusual and the clinical importance of clostridium bacteremia remains unclear. this retrospective case study examined the risk factors among a group of patients who developed clostridium bacteremia. medical records from 73 episodes of clostridial bacteremia in 73 patients treated in a medical center during an 11-year period were reviewed. of all episodes, 96% were community-acquired. twelve percent of patients had polymicrobial bacteremia, with escheri ... | 2001 | 11456356 |
| tetracycline-resistance genes of clostridium perfringens, clostridium septicum and clostridium sordellii isolated from cattle affected with malignant edema. | the minimal inhibitory concentrations (mics) of 10 antimicrobial agents against a total of 33 isolates of clostridium perfringens, clostridium septicum and clostridium sordellii from cattle affected with malignant edema in japan was determined. the low mic activities of benzylpenicillin confirm the place of benzylpenicillin as the antibiotics of choice for treatment of malignant edema. five (22%) of 23 c. septicum strains, five (71%) of seven c. perfringens strains and all strains of c. sordelli ... | 2001 | 11524166 |
| differences in the pathogenicity of various bacterial isolates used in an induction model for gangrenous dermatitis in broiler chickens. | a gangrenous dermatitis model was developed in broiler chickens, in which birds previously vaccinated at 14 days of age with a bursal disease virus vaccine were challenged at 4 wk of age with various bacterial combinations with the combination of subcutaneous and intramuscular injection. gangrenous dermatitis lesions were not produced in birds injected with one of the staphylococcus aureus isolates, either alone or in combination with various clostridium septicum isolates. other s. aureus isolat ... | 2001 | 11569740 |
| sudden death of a bearded vulture (gypaetus barbatus) possibly caused by newcastle disease virus. | an adult female bearded vulture (gypaetus barbatus) in the tel aviv university research zoo was found dead without previous clinical signs. the predominant pathologic changes were considerable bloody content in the intestines and enlargement of the liver, which had a rubbery consistency with color changes. microscopic lesions consisted of multifocal histiocytic infiltration in the liver. newcastle disease virus (ndv) was isolated from a cloacal swab and from the lungs and liver. intracerebral pa ... | 2001 | 11569753 |
| clostridium perfringens beta toxin and clostridium septicum alpha toxin: their mechanisms and possible role in pathogenesis. | the clostridium septicum alpha toxin and the clostridium perfringens beta toxin are examples of pore-forming toxins that exhibit several different features. the cell types that are targeted by these toxins reflect the effect these toxins have on the host during infection with either organism. alpha toxin elicits a rapid shock-like syndrome, whereas beta toxin appears to induce a variety of neurological effects. the effects of the purified toxins appear to mimic some of the features of the animal ... | 2001 | 11423190 |
| toxin synthesis and mucin breakdown are related to swarming phenomenon in clostridium septicum. | clostridium septicum is responsible for several diseases in humans and animals. the bacterium is capable of a simple kind of multicellular behavior known as swarming. in this investigation, environmental and physiologic factors affecting growth and swarm cell formation in c. septicum were studied over a range of dilution rates (d = 0.02 to 0.65 h(-1)) in glucose-limited, glucose-excess, and mucin-limited chemostats. cellular differentiation was observed at low specific growth rates, irrespective ... | 2001 | 11160009 |
| rapid detection and identification of clostridium chauvoei by pcr based on flagellin gene sequence. | we developed a one-step polymerase chain reaction (pcr) system that specifically detects clostridium chauvoei. oligonucleotide primers were designed to amplify a 516-bp fragment of the structural flagellin gene. the specificity of the pcr was investigated by analyzing 59 strains of clostridia, and seven strain of other genera. a 516-bp fragment could be amplified from all the c. chauvoei strains tested, and no amplification was observed by using dnas from the other strains tested, including clos ... | 2001 | 11182502 |
| spontaneous bifocal clostridium septicum gas gangrene. | clostridium septicum gas gangrene (myonecrosis) is an acutely painful and rapidly fatal infection occurring in the absence of trauma. urgent surgery is essential both to control pain and to ensure survival. most patients who develop this infection have an underlying malignancy and clinicians should be aware of this association. we present a case of bifocal myonecrosis which to our knowledge has not been reported previously. | 2001 | 11245518 |
| [paraneoplastic clostridium septicum bacteremia]. | 2001 | 11333609 | |
| spontaneous bifocal clostridium septicum gas gangrene. | 2001 | 11380147 | |
| risk factors for anaerobic bloodstream infections in bone marrow transplant recipients. | the incidence of anaerobic bloodstream infections (bsi) in patients who underwent bone marrow transplantation (bmt) recently increased at our institution. a retrospective case-control study of patients undergoing bmt from january 1995 through december 1998 was performed to determine the microbiological characteristics, epidemiology, and outcome of anaerobic bsi and to identify independent risk factors for infection. anaerobic bsi occurred in 23 patients, for a rate of 4 bsis per 100 bmt procedur ... | 2001 | 11438899 |
| clostridial mycotic aneurysm of the thoracoabdominal aorta--a case report. | clostridial infection of the aorta is a rare and life-threatening condition. the management of a mycotic aneurysm involving the thoracoabdominal aorta due to clostridium septicum infection is presented. successful surgical management of the aortic infection involved arterial resection, wide debridement of the surrounding tissues, and in situ graft replacement. sixteen additional cases of clostridial infection of the aortoiliac segment reported in the literature are also summarized. in ten of the ... | 2001 | 11586456 |
| [clostridium septicum infection and cancer]. | unusual infections may occasionally be the first sign of cancer. | 2001 | 11603046 |