regulation of trek1 expression in nasal mucosa with allergic rhinitis by specific immunotherapy.epithelial barrier dysfunction is involved in the pathogenesis of allergic disorders, such as nasal allergy. twik-related k(+) 1 (trek1) potassium channels are required in the maintenance of the epithelial barrier function. this study aims to investigate the role of antigen-specific immunotherapy (sit) in the regulation of trek1 expression in the nasal mucosa. in this study, patients with nasal allergy were treated with sit and/or clostridium butyricum. the expression of trek1 and histone demeth ...201525529528
effect of 1,3-propanediol, organic acids, and ethanol on growth and metabolism of clostridium butyricum dsp1.knowledge of tolerance of bacteria to toxic stress is important, especially for processes targeted at high final titers of product. information on environmental limits and stress responses may help during selection of strains or design and control of processes. the influence of the main product and its co-products on the process of 1,3-propanediol (pd) synthesis was determined. adaptation to toxic compounds was noticed as clostridium butyricum dsp1 was less sensitive to the addition of these fac ...201525524700
biotechnological potential of clostridium butyricum response to demand from industry for microorganisms with auspicious biotechnological potential, a worldwide interest has developed in bacteria and fungi isolation. microorganisms of interesting metabolic properties include non-pathogenic bacteria of the genus clostridium, particularly c. acetobutylicum, c. butyricum and c. pasteurianum. a well-known property of c. butyricum is their ability to produce butyric acid, as well as effectively convert glycerol to 1,3-propanediol (38.2 g/l). a conve ...201425477923
hypothetical glycerol pathways of newly isolated strains capable of 1,3-propanediol presented here demonstrates the ability of three newly isolated strains, obtained from environmental probes (manure, bottom sediment, and food waste) and identified as clostridium bifermentans, clostridium butyricum, and hafnia alvei, to synthesize 1,3-propanediol (1,3-pd), organic acids (such as lactic, acetic, fumaric, succinic, and butyric acids), and ethanol from glycerol. the production of 1,3-pd as well as the glycerol pathways in c. bifermentans and h. alvei cells have not been inve ...201425473655
flux balance analysis of different carbon source fermentation with hydrogen producing clostridium butyricum using cell net analyzer.a metabolic network model for clostridium butyricum was developed using six different carbon sources (sucrose, fructose, galactose, mannose, trehalose and ribose) to study the fermentative h2 production. the model was used for investigation of h2 production and the ability of growth on different substrates to predict the maximum abilities for fermentative h2 production that each substrate can support. nadh fluxes were calculated by the model as an important cofactor affecting on h2 production. b ...201525453441
exploring the genome of a butyric acid producer, clostridium butyricum incqs635.the draft genome sequence of clostridium butyricum incqs635 was obtained by means of ion sequencing. the genome provides further insight into the genetic repertoire involved with metabolic pathways related to the fermentation of different compounds and organic solvents synthesis (i.e., butyric acid) with biofuel applications.201425414496
infant botulism due to c. butyricum type e toxin: a novel environmental association with pet terrapins.we describe two cases of infant botulism due to clostridium butyricum producing botulinum type e neurotoxin (bont/e) and a previously unreported environmental source. the infants presented at age 11 days with poor feeding and lethargy, hypotonia, dilated pupils and absent reflexes. faecal samples were positive for c. butyricum bont/e. the infants recovered after treatment including botulism immune globulin intravenous (big-iv). c. butyricum bont/e was isolated from water from tanks housing pet ' ...201525306863
16s rrna gene sequencing, multilocus sequence analysis, and mass spectrometry identification of the proposed new species "clostridium neonatale".in 2002, an outbreak of necrotizing enterocolitis in a canadian neonatal intensive care unit was associated with a proposed novel species of clostridium, "clostridium neonatale." to date, there are no data about the isolation, identification, or clinical significance of this species. additionally, c. neonatale has not been formally classified as a new species, rendering its identification challenging. indeed, the c. neonatale 16s rrna gene sequence shows high similarity to another clostridium sp ...201425232167
crystal structure of (s)-3-hydroxybutyryl-coa dehydrogenase from clostridium butyricum and its mutations that enhance reaction kinetics.3-hydroxybutyryl-coa dehydrogenase is an enzyme that catalyzes the second step in the biosynthesis of n-butanol from acetyl-coa, in which acetoacetyl-coa is reduced to 3-hydroxybutyryl-coa. to understand the molecular mechanisms of n-butanol biosynthesis, we determined the crystal structure of 3-hydroxybutyryl-coa dehydrogenase from clostridium butyricum (cbhbd). the monomer structure of cbhbd exhibits a two-domain topology, with n- and c-terminal domains, and the dimerization of the enzyme was ...201425112316
multiplicity of steady states in a bioreactor during the production of 1,3-propanediol by clostridium recent decades, the production of compounds from microorganisms has increased significantly. glycerol as a source of substrate appears to have great potential, due to its large supply because of the increase in biodiesel production. this paper will discuss the multiplicity of steady states for the production of 1,3-propanediol from glycerol by clostridium butyricum, employing a model that takes into account inhibition by fermentation products. the theoretical study of bifurcation enabled us t ...201525090981
genome sequence of the neurotoxigenic clostridium butyricum strain 5521.clostridium strains from six phylogenetic groups, c. botulinum groups i to iv, c. baratii, and c. butyricum, display the capacity to produce botulinum neurotoxin. here, we present the genome sequence of a c. butyricum isolate, the neurotoxigenic strain 5521, which encodes the type e botulinum neurotoxin.201424970833
[warfarin effects diminishment by the clostridium butyricum preparation after cardiac surgery; report of a case].a 55-year-old gentleman diagnosed with severe aortic insufficiency and annuloaortic ectasia underwent modified bentall procedure using mechanical valve. anticoagulation therapy with warfarin was introduced after surgery, but sufficient anticoagulation effect was not achieved with a large amount of dosage. after discontinuing the use of the clostridium butyricum preparation on postoperative day 29, sufficient anticoagulationt effect was obtained within several days. clostridium butyricum might ch ...201424917411
clostridium butyricum reduce lipogenesis through bacterial wall components and butyrate.intervention strategies for obesity are global issues that require immediate attention. the objective of this study was to assess the possibility that clostridium butyricum and its potential components could reduce lipogenesis. co-culture experiments of caco-2 cells and 1 × 10(6), 1 × 10(7), and 1 × 10(8) cfu/ml of c. butyricum were set up to monitor the cytotoxicity of c. butyricum and the changes of angiopoietin-like protein 4 (angptl4) mrna expression. it was found that cell viability was not ...201424878750
impact of organic loading rate on biohydrogen production in an up-flow anaerobic packed bed reactor (uanpbr).this study assesses the impact of organic loading rate on biohydrogen production from glucose in an up-flow anaerobic packed bed reactor (uanpbr). two mesophilic uapbrs (uanpbr1 and 2) were tested at organic loading rates (olrs) ranging from 6.5 to 51.4 g cod l(-1)d(-1). to overcome biomass washout, design modifications were made in the uanpbr2 to include a settling zone to capture the detached biomass. the design modifications in uanpbr2 increased the average hydrogen yield from 0.98 to 2.0 mol ...201424865326
antibacterial activity against clostridium genus and antiradical activity of the essential oils from different the present study, the antimicrobial and antiradical activities of 15 essential oils were investigated. the antimicrobial activities were determined by using agar disc diffusion and broth microdilution methods against clostridium genus and antioxidant properties of essential oils by testing their scavenging effect on dpph radicals activities. we determined the antibacterial activity of clostridium butyricum, clostridium hystoliticum, clostridium intestinale, clostridium perfringens and clostr ...201424813985
effects of a bacteria-based probiotic on ruminal ph, volatile fatty acids and bacterial flora of holstein calves.twelve ruminally cannulated holstein calves (age, 12 ± 3 weeks) were used to identify the effect of a probiotic comprised of lactobacillus plantarum, enterococcus faecium and clostridium butyricum on ruminal components. the calves were adapted to a diet containing a 50% high-concentrate (standard diet) for 1 week, and then, the probiotic was given once daily for 5 days (day 1-5) at 1.5 or 3.0 g/100 kg body weight to groups of four calves each. four additional calves fed the standard diet without ...201424614603
effects of clostridium butyricum on growth performance, immune function, and cecal microflora in broiler chickens challenged with escherichia coli k88.this study was conducted to investigate the effects of clostridium butyricumon growth performance, immune function, and cecal microflora in broiler chickens challenged with escherichia coli k88. three hundred sixty 1-d-old broiler chickens were randomly divided into 4 treatments: negative control (nc) birds were fed a basal diet and not challenged with e. coli k88; positive control (pc) birds were fed a basal diet and challenged with e. coli k88; c. butyricum treatment (cb) birds were fed a diet ...201424570422
direct bioconversion of raw corn stalk to hydrogen by a new strain clostridium sp. fs3.a new strain fs3 which could achieve an efficient bioconversion of raw corn stalk to hydrogen had been isolated from anaerobic acclimated sludge, and identified as clostridium butyricum on the basis of a series of physiological and biochemical experiments and 16s rdna gene sequence. the strain could utilize various carbon sources to produce hydrogen. on the basis of single-factor experiments, the response surface methodology (rsm) was performed to optimize the media for hydrogen production. the ...201424534789
characterization of romboutsia ilealis gen. nov., sp. nov., isolated from the gastro-intestinal tract of a rat, and proposal for the reclassification of five closely related members of the genus clostridium into the genera romboutsia gen. nov., intestinibacter gen. nov., terrisporobacter gen. nov. and asaccharospora gen. nov.a gram-positive staining, rod-shaped, non-motile, spore-forming obligately anaerobic bacterium, designated cribt, was isolated from the gastro-intestinal tract of a rat and characterized. the major cellular fatty acids of strain cribt were saturated and unsaturated straight-chain c12-c19 fatty acids, with c16:0 being the predominant fatty acid. the polar lipid profile comprised six glycolipids, four phospholipids and one lipid that did not stain with any of the specific spray reagents used. the ...201424480908
prolonged antibiotic use induces intestinal injury in mice that is repaired after removing antibiotic pressure: implications for empiric antibiotic therapy.metabolic profiling of urine and fecal extracts, histological investigation of intestinal ilea, and fecal metagenomics analyses were used to investigate effects of prolonged antibiotic use in mice. the study provides insight into the effects of extended empiric antibiotic therapy in humans. mice were administered a broad-spectrum antibiotic for four consecutive days followed by oral gavage with clostridium butyricum, an opportunistic gram-positive pathogenic bacteria commonly isolated in fecal a ...201426273236
immune-stimulatory effects of a bacteria-based probiotic on peripheral leukocyte subpopulations and cytokine mrna expression levels in scouring holstein calves.subpopulations of peripheral leukocytes and cytokine mrna expression levels were evaluated in scouring and healthy holstein calves (age 10 ± 5 days; n=42) treated with a probiotic consisting of lactobacillus plantarum, enterococcus faecium and clostridium butyricum. the calves were assigned to the scouring or healthy group and then subdivided into pathogen-positive treated (n=8), pathogen-positive control (n=8), pathogen-negative treated (n=6), pathogen-negative control (n=6), healthy treated (n ...201424451928
a gene-targeted approach to investigate the intestinal butyrate-producing bacterial community.butyrate, which is produced by the human microbiome, is essential for a well-functioning colon. bacteria that produce butyrate are phylogenetically diverse, which hinders their accurate detection based on conventional phylogenetic markers. as a result, reliable information on this important bacterial group is often lacking in microbiome research.201324451334
acid tolerance response (atr) of microbial communities during the enhanced biohydrogen process via cascade acid stress.enhanced biohydrogen production via cascade acid stress on microbial communities, structure patterns of the microbial communities revealed by plfas, and the succession of biohydrogen related species against cascade acid stress were all investigated. it was found that hydrogen production could be improved from 48.7 to 79.4ml/gvs after cascade acid stress. in addition, the gram negative (g(-)) bacteria were found to be more tolerant to organic acids than those of the gram positive (g(+)) bacteria, ...201424434699
in vitro protective efficacy of clostridium butyricum against fish pathogen infections.most pathogens in intestine are opportunist, called "opportunistic pathogens" that usually do not cause disease in a healthy host. only when the host's resistance is lowered or the intestinal microecological balance is destroyed, the opportunistic pathogens are capable of causing disease. here, two opportunistic pathogens, salmonella enteritidis and vibrio parahaemolyticus were chosen to test the possible antagonistic effect of the probiotic agent clostridium butyricum on these pathogens infecti ...201324426150
growth limiting ph, water activity, and temperature for neurotoxigenic strains of clostridium butyricum.some rare strains of clostridium butyricum carry the gene encoding the botulinal type e neurotoxin and must be considered as possible hazards in certain types of food. the limiting growth conditions for c. butyricum were determined in peptone yeast glucose starch (pygs) broth incubated anaerobically at 30°c for up to 42 days. the minimum ph values permitting growth depended on the acidulant and strain. organic acids were more effective at inhibiting growth than hcl as expected. the lowest ph val ...201324195005
clostridium butyricum miyairi 588 improves high-fat diet-induced non-alcoholic fatty liver disease in rats.non-alcoholic fatty liver disease (nafld) has become a common liver disease, as its prevalence has increased markedly in recent decades. the aim of the present study was to examine the improving effect of clostridium butyricum miyairi 588 (cbm588), a probiotic in clinical use for antibiotic-associated diarrhea, against high-fat diet (hfd)-induced fatty liver in rats.201324166662
effects of a bacteria-based probiotic on subpopulations of peripheral leukocytes and their cytokine mrna expression in calves.eight holstein calves (10 ± 3 weeks) were used to examine the interaction between a bacteria-based probiotic agent (probiotic) and the function of peripheral blood mononuclear cells (pbmcs). the probiotic, consisting of lactobacillus plantarum, enterococcus faecium and clostridium butyricum, was administered orally at 3.0 g/100 kg body weight to calves once daily for 5 consecutive days. calves given the vehicle alone with no probiotic served as the control. in the treatment group, increases in n ...201424131856
batch fermentative hydrogen production by enriched mixed culture: combination strategy and their microbial composition.the effect of individual and combined mixed culture on dark fermentative hydrogen production performance was investigated. mixed cultures from cow dung (c1), sewage sludge (c2), and pig slurry (c3) were enriched under strict anaerobic conditions at 37°c with glucose as the sole carbon source. biochemical hydrogen production test in peptone-yeast-glucose (pyg) and basal medium was performed for individual mixed cultures (c1, c2 and c3) and their combinations (c1-c2, c2-c3, c1-c3 and c1-c2-c3) at ...201424095211
genome sequence of clostridium butyricum strain dsm 10702, a promising producer of biofuels and biochemicals.clostridium butyricum strains have been considered promising producers of biofuels and biochemicals, such as hydrogen, butanol, butyric acid, and 1,3-propanediol. here, we present a 4.59-mb assembly of the genome sequence of dsm 10702 (vpi 3266), a type strain of c. butyricum.201323908293
biohydrogen production from microalgal biomass: energy requirement, co2 emissions and scale-up scenarios.this paper presents a life cycle inventory of biohydrogen production by clostridium butyricum through the fermentation of the whole scenedesmus obliquus biomass. the main purpose of this work was to determine the energy consumption and co2 emissions during the production of hydrogen. this was accomplished through the fermentation of the microalgal biomass cultivated in an outdoor raceway pond and the preparation of the inoculum and culture media. the scale-up scenarios are discussed aiming for a ...201323867534
antimicrobial susceptibility of enterococcus strains used in clinical practice as probiotics.the aim of this study was to evaluate the antimicrobial susceptibilities of probiotic strains that are suggested to be effective for preventing antibiotics-associated diarrhea (aad). the minimum inhibitory concentrations (mics) of 17 antibiotics against probiotic strains were tested by the agar plate dilution method or broth microdilution method. in all, eight probiotic strains containing enterococcus faecalis, bifidobacterium spp., clostridium butyricum, and lactobacillus acidophilus were teste ...201323775143
a single strain of clostridium butyricum induces intestinal il-10-producing macrophages to suppress acute experimental colitis in mice.imbalance in gut bacterial composition provokes host proinflammatory responses causing diseases such as colitis. colonization with a mixture of clostridium species from clusters iv and xiva was shown to suppress colitis through the induction of il-10-producing regulatory t (treg) cells. we demonstrate that a distinct clostridium strain from cluster i, clostridium butyricum (cb), prevents acute experimental colitis in mice through induction of il-10, an anti-inflammatory cytokine. however, while ...201323768495
enhancement effect of silver nanoparticles on fermentative biohydrogen production using mixed bacteria.silver nanoparticles were added into anaerobic batch reactors to enhance acidogenesis and fermentative hydrogen production simultaneously. the effects of silver nanoparticles concentration (0-200 nmol l(-1)) and inorganic nitrogen concentration (0-4.125 g l(-1)) on cell growth and hydrogen production were investigated using glucose-fed mixed bacteria dominated by clostridium butyricum. the tests with silver nanoparticles exhibited much higher h2 yields than the blank, and the maximum hydrogen yi ...201323743428
effects of clostridium butyricum and enterococcus faecium on growth performance, lipid metabolism, and cecal microbiota of broiler investigate the effects of clostridium butyricum and enterococcus faecium on the growth performance, lipid metabolism, and cecal microbiota of broilers, 264 one-day-old male ross 308 broiler chicks were randomly allocated into four treatments with six replicates in a 2 × 2 factorial arrangement and fed four diets with two levels of c. butyricum (0 or 1 × 10⁹ cfu/kg) and two levels of e. faecium (0 or 2 × 10⁹ cfu/kg) for 42 days. there was no significant interaction between c. butyricum and e. ...201323666481
short communication: jenny milk as an inhibitor of late blowing in cheese: a preliminary report.late blowing on semihard and hard cheese may have an important economic effect on dairy production. many studies have attempted to prevent this defect by physical treatment, the use of additives, and the use of bacteriocins. in this paper, we look at the effect of jenny milk as an inhibitor of blowing caused by clostridia and coliforms in ewe cheese making. bulk ewe and jenny milk samples were collected in the morning by mechanical milking and were refrigerated at 4°c. on the collected samples, ...201323587377
improving effect of metal and oxide nanoparticles encapsulated in porous silica on fermentative biohydrogen production by clostridium butyricum.this paper investigated the enhancement effect of nanometre-sized metallic (pd, ag and cu) or metallic oxide (fexoy) nanoparticles on fermentative hydrogen production from glucose by a clostridium butyricum strain. these nanoparticles (np) of about 2-3 nm were encapsulated in porous silica (sio2) and were added at very low concentration (10(-6) mol l(-1)) in batch hydrogen production test. the cultures containing iron oxide np produced 38% more hydrogen with a higher maximum h2 production rate ( ...201323428815
clostridium butyricum miyairi 588 shows antitumor effects by enhancing the release of trail from neutrophils through mmp-8.bacillus calmette-guérin (bcg) intravesical therapy against superficial bladder cancer is one of the most successful immunotherapies in cancer, though the precise mechanism has not been clarified. recent studies have demonstrated urinary tumor necrosis factor-related apoptosis-inducing ligand (trail) levels to be higher in bcg-responsive patients than non-responders and shown that polymorphonuclear neutrophils (pmns) migrating to the bladder after bcg instillation release large amounts of trail. ...201323354042
photoheterotrophic growth of chlorella vulgaris esp6 on organic acids from dark hydrogen fermentation this work, chlorella vulgaris esp6 was used to assimilate the soluble metabolites in the hydrogen fermentation broth of clostridium butyricum cgs5 to obtain valuable microalgae biomass. the results show that c. vulgaris esp6 could grow on the 4-fold diluted dark fermentation broth. acetate was efficiently utilized during the growth of c. vulgaris esp6, whereas the microalgae growth was inhibited by lactate, butyrate, and hco3(-) when their concentrations were higher than 0.5, 0.1, and 2.72 g/ ...201323305898
[multicenter, randomized, controlled clinical trial on preventing antibiotic-associated diarrhea in children with pneumonia using the live clostridium butyricum and bifidobacterium combined powder].many previous meta-analysis have shown that the probiotics can lower the incidence of antibiotic-associated diarrhea (aad) in children. however, the function and efficacy of probiotics drugs showed obvious strains specificity and dose dependence. currently, most of the reported meta-analysis regarding probiotics aad prevention have adopted saccharomyces, lactobacilleae, streptococcus and bifidobacterium. this study aimed to evaluate the efficacy and safety of the live clostridium butyricum and b ...201223302558
the potential of bacteria isolated from ruminal contents of seaweed-eating north ronaldsay sheep to hydrolyse seaweed components and produce methane by anaerobic digestion in vitro.the production of methane biofuel from seaweeds is limited by the hydrolysis of polysaccharides. the rumen microbiota of seaweed-eating north ronaldsay sheep was studied for polysaccharidic bacterial isolates degrading brown-seaweed polysaccharides. only nine isolates out of 65 utilized >90% of the polysaccharide they were isolated on. the nine isolates (eight prevotella spp. and one clostridium butyricum) utilized whole laminaria hyperborea extract and a range of seaweed polysaccharides, includ ...201323170956
effects of probiotic, clostridium butyricum, on growth performance, immune function, and cecal microflora in broiler chickens.four hundred and fifty 1-d-old male lingnan yellow broiler chickens were used to investigate the effects of clostridium butyricum on growth performance, immune function, and cecal microflora. the birds were randomly assigned to 5 treatments and offered the same antibiotic-free basal diets for 42 d. the treatments were as follows: no addition (control), 1 × 10(7) cfu c. butyricum/kg of diet (cb1), 2 × 10(7) cfu c. butyricum/kg of diet (cb2), 3 × 10(7) cfu c. butyricum/kg of diet (cb3), and 10 mg ...201222912445
1,3-propanediol production by escherichia coli expressing genes of dha operon from clostridium butyricum 2cr371.5.1,3-propanediol is used as a monomer in the production of some polymers e.g. polytrimethylene terephthalate used in the production of carpets and textile fibers and in the thermoplastics engineering. however, the traditional chemical synthesis is expensive, generates some toxic intermediates and requires a reduction step under high hydrogen pressure. biological production of 1,3-propanediol could be an attractive alternative to the traditional chemical methods. moreover, crude glycerol which is ...201222855718
screening of environmental samples for bacteria producing 1,3-propanediol from glycerol.twenty nine environmental samples were screened for the presence of anaerobic microorganisms fermenting glycerol with 1,3-propanediol as a final product. seven samples were then selected for the next step of our research and eight bacteria strains were cultured anaerobically. seven of them produced 1,3-propanediol with a yield of 0.47-0.58. six of the the isolated microorganisms were then classified as clostridium butyricum (four strains), c. lituseburense (one strain), and c. sartagoforme (one ...201222855717
[susceptibility of spore-forming butyric acid bacteria to antimicrobial agents].antimicrobial agents occasionally cause certain adverse effects, such as diarrhea and loose stool, by altering the composition of the intestinal flora. antibiotic-resistant lactic acid bacteria are used to prevent these adverse effects. although these bacteria are not resistant to several recently introduced antimicrobial agents, bacterial preparations are still sometimes prescribed concomitantly with these antimicrobial agents. in this study, we investigated whether the administration of the sp ...201222790032
ability of clostridium butyricum to inhibit escherichia coli-induced apoptosis in chicken embryo intestinal cells.the beneficial effects of clostridium butyricum in the treatment of intestinal inflammatory disorders are well known. however, it is not fully understood how such bacteria inhibit pathogen-induced intestinal diseases. for this purpose, we investigated the effects of c. butyricum and its spent culture supernatants (scs) on escherichia coli (ehec) growth and adherence to chicken embryo intestinal cells (ceics). we also evaluated the potential of c. butyricum to inhibit ehec-induced apoptosis in ce ...201222771207
a genetic and metabolic approach to redirection of biochemical pathways of clostridium butyricum for enhancing hydrogen production.clostridium butyricum, a well known h(2) producing bacterium, produces lactate, butyrate, acetate, ethanol, and co(2) as its main by-products from glucose. the conversion of pyruvate to lactate, butyrate and ethanol involves oxidation of nadh. it was hypothesized that the nadh could be increased if the formation of these by-products could be eliminated, resulting in enhancing h(2) yield. herein, this study aimed to establish a genetic and metabolic approach for enhancing h(2) yield via redirecti ...201322753004
gaseous co2 signal initiates growth of butyric-acid-producing clostridium butyricum in both pure culture and mixed cultures with lactobacillus brevis.microbial strains produce numerous volatile substances in the anaerobic conditions of the human intestines. the availability of co(2) is known to be a prerequisite for bacterial growth in general. in experiments with anaerobic lactobacillus brevis and clostridium butyricum bacteria in the portable microbial enrichment unit (pmeu) it was shown that these strains interact; this interaction being mediated by co(2) emission. co(2) promoted clostridial growth in pure cultures and mixed cultures with ...201222697044
an important role of interleukin-10 in counteracting excessive immune response in ht-29 cells exposed to clostridium butyricum.clostridium butyricum has become increasingly important in preventing and treating intestinal inflammation. in the intestine it may increase the resistance of the gut to pathogen invasion via inducing the secretion of anti-inflammatory cytokines. interleukin 10 (il-10) plays a central role in preventing certain inflammatory diseases by down-regulating inflammatory cascades. in a previous study, we observed that the level of il-10 mrna was modulated by c. butyricum. the aim of this study was to i ...201222681958
sodium (na+) concentration effects on metabolic pathway and estimation of atp use in dark fermentation hydrogen production through stoichiometric analysis.batch experiments were conducted to investigate the effects of na(+) concentration on hydrogen production with dark fermentation. the na(+) concentration was varied from 0 to 8 g/l in the mixed culture using an anaerobic sludge treated by acid. the maximum hydrogen production was achieved with 1 g-na(+)/l, whereas the hydrogen production was decreased over 2 g-na(+)/l due to the inhibitory of na(+). the mechanisms of na(+) inhibition to the hydrogen production are studied using pure culture of c ...201222634156
[prolonged cultivation of an anaerobic bacterial community producing hydrogen].this paper studies various methods of long-term maintenance of the process of hydrogen evolution during the growth of an aerobic bacterial community on a starch-containing environment. when cultured in separable trip fermentation mode for 72 days, from 0.10 to 0.23 h2/l of medium/day was formed. the regime of regular reseeding lasted more than 100 days, forming an average of 0.81 1 h2/l of medium/day. the advantages and disadvantages of different methods of microbial hydrogen production during a ...201322586916
adaptation dynamics of clostridium butyricum in high 1,3-propanediol content media.aim of the present study was to evaluate the effect of exogenous additions of 1,3-propanediol (1,3-pdo) on microbial growth and metabolites production of clostridium butyricum vpi 1718 strain, during crude glycerol fermentation. preliminary batch cultures in anaerobic duran bottles revealed that early addition of 1,3-pdo caused growth cessation in rather low quantities (15 g/l), while 1,3-pdo additions during the middle exponential growth phase up to 70 g/l resulted in an almost linear decrease ...201222456628
effects of ph and carbon sources on biohydrogen production by co-culture of clostridium butyricum and rhodobacter improve the hydrogen yield from biological fermentation of organic wastewater, a co-culture system of dark- and photo-fermentation bacteria was investigated. in a pureculture system of the dark-fermentation bacterium clostridium butyricum, a ph of 6.25 was found to be optimal, resulting in a hydrogen production rate of 18.7 ml-h₂/l/h. on the other hand, the photosynthetic bacterium rhodobacter sphaeroides could produce the most hydrogen at 1.81 mol-h₂/mol-glucose at ph 7.0. the maximum specif ...201222450797
effects of dietary lipids and clostridium butyricum on serum lipids and lipid-related gene expression in broiler chickens.the effect of dietary lipids and clostridium butyricum supplementation on circulating lipids and the transcription of selected genes involved in lipid metabolism were investigated in broiler chickens. one hundred and sixty-day-old broilers (arbor acres) were divided into four treatment groups in a 2 × 2 factorial arrangement and fed four diets with two lipid sources (soybean oil or fish oil) and two levels of c. butyricum (0 or 5 g/kg) for a period of 6 weeks. serum concentrations of total chole ...201122440467
oil palm empty fruit bunch as alternative substrate for acetone-butanol-ethanol production by clostridium butyricum eb6.acetone-butanol-ethanol (abe) production from renewable resources has been widely reported. in this study, clostridium butyricum eb6 was employed for abe fermentation using fermentable sugar derived from treated oil palm empty fruit bunch (opefb). a higher amount of abe (2.61 g/l) was produced in a fermentation using treated opefb as the substrate when compared to a glucose based medium that produced 0.24 g/l at ph 5.5. abe production was increased to 3.47 g/l with a yield of 0.24 g/g at ph 6.0. ...201222391689
effect of probiotics on serum bile acids in patients with ulcerative colitis.evaluation of bile acids (ba) is useful for assessing the changes of intestinal flora in patients with ulcerative colitis (uc). during enterohepatic circulation, the intestinal micro flora cause 7 alpha-dehydroxylation of cholic acid (ca) and chenodeoxycholic acid (cdca), yielding deoxycholic acid(dca) and lithocholic acid, respectively. the aim of the present study was to investigate the effects of probiotics in patients with uc by examining changes of the serum ba profile.201222369744
[engineering of escherichia coli for convenient expression of [fefe]-hydrogenase].a new method used to heterologously express [fefe]-hydrogenase in escherichia coli was investigated in our present study.201122260044
effect of growth supplements and whey pretreatment on butyric acid production by clostridium butyricum.improved production of butyrate (up to 19 g/l) from whey by clostridium butyricum was achieved by adding either yeast extract (5 g/l) or biotin (50 μg/l). hydrolysed lactose and proteolysed whey were less effective even with added biotin.199524414673
iron reduction and gley formation by nitrogen-fixing clostridia.studies on iron reduction and the mechanism of gley formation by nitrogen-fixing clostridia are reported. up to 10(6) cells/g soil of anaerobic, nitrogen-fixing clostridia, capable of reducing iron (iii)-oxide, were counted in samples taken from various top soils. in a gleyed subsoil as many as 10(5) bacteria per g soil, capable of reducing and fixing nitrogen, were enumerated using the most probable number technique. in general, the ratio of the auxotrophic iron reducing clostridia (glucose+yea ...197128310760
mixed-culture h2 fermentation performance and the relation between microbial community composition and hydraulic retention times for a fixed bed reactor fed with galactose/glucose mixtures.this study examined the mesophilic continuous biohydrogen fermentation from galactose and glucose mixture with an initial substrate concentration of 15 g/l (galactose 12 g/l and glucose 3 g/l) as a resembling carbon source of pretreated red algal hydrolyzate. a fixed bed reactor was fed with the sugar mixture at various hydraulic retention times (hrts) ranging 12 to 1.5 h. the maximum hydrogen production rate of 52.6 l/l-d was found at 2 h hrt, while the maximum hydrogen yield of 2.3±0.1 mol/mol ...201728528789
administration of lactobacillus salivarius li01 or pediococcus pentosaceus li05 prevents ccl4-induced liver cirrhosis by protecting the intestinal barrier in rats.alterations in the gut microbiome have been reported in liver cirrhosis, and probiotic interventions are considered a potential treatment strategy. this study aimed to evaluate the effects and mechanisms of lactobacillus salivarius li01, pediococcus pentosaceus li05, lactobacillus rhamnosus gg, clostridium butyricum miyairi and bacillus licheniformis zhengchangsheng on ccl4-induced cirrhotic rats. only administration of li01 or li05 prevented liver fibrosis and down-regulated the hepatic express ...201728761060
efficacy and safety of probiotic-supplemented triple therapy for eradication of helicobacter pylori in children: a systematic review and network meta-analysis.the aim of this study was to identify the best probiotic supplementation in triple therapy for pediatric population with helicobacter pylori infection.201728681177
finished whole-genome sequences of clostridium butyricum toxin subtype e4 and clostridium baratii toxin subtype f7 strains.clostridium butyricum and clostridium baratii species have been known to produce botulinum toxin types e and f, respectively, which can cause botulism, a rare but serious neuroparalytic disease. here, we present finished genome sequences for two of these clinically relevant strains.201728729254
protection mechanism of clostridium butyricum against salmonella enteritidis infection in broilers.this study was designed to evaluate the protection mechanism of oral administration of clostridium butyricum against salmonella enteritidis (se) colonization in broilers. in the current study, 180 one-day-old healthy arbor acres (aa) broilers were meanly grouped into three, with three replicates of 20 birds each. an negative control group was fed basal diet without se challenge and a positive control (pc) group was fed the basal diet and challenged with se [10(6) colony forming unit (cfu)/0.2 ml ...201728848530
exploring the microbial origins of p-cresol and its co-occurrence pattern in the chinese liquor-making process.the compound p-cresol is the major off-odor and toxic component of strong aroma-type chinese liquor. to trace its origin, the p-cresol contents in the liquor-making process were detected by gas chromatography-mass spectrometry. the prokaryotic communities involved in the process were revealed by 16s rrna gene miseq sequencing. the results showed that the microbial diversity and concentration of p-cresol in pit mud significantly increased with increased pit depth. canonical correspondence analysi ...201728843121
effects of polysaccharide from physalis alkekengi var. francheti on liver injury and intestinal microflora in type-2 diabetic mice.diabetic liver injury is a serious diabetic complication. the alterations of intestinal microbiota play an important role in induction and promotion of liver injury progression. physalis alkekengi l. var. francheti (mast.) makino (solanaceae) has been used as a water decoction for treating diabetes.201728832229
anti-diabetic effects of clostridium butyricum cgmcc0313.1 through promoting the growth of gut butyrate-producing bacteria in type 2 diabetic mice.patients with type 2 diabetes (t2d) have decreased butyrate-producing bacteria. we hypothesized that supplementation with butyrate-producing bacteria may exert beneficial effects on t2d. the current study investigated the effects of well-characterized butyrate-producing bacteria clostridium butyricum cgmcc0313.1 (cb0313.1) on hyperglycemia and associated metabolic dysfunction in two diabetic mouse models. cb0313.1 was administered daily by oral gavage to leptin (db/db) mice for 5 weeks starting ...201728765642
description of absiella argi gen. nov., sp. nov., and transfer of eubacterium dolichum and eubacterium tortuosum to the genus absiella as absiella dolichum comb. nov. and absiella tortuosum comb. nov.gram-positive, straight or slightly curved rod-shaped bacteria, designated as strains n6h1-5(t) and n6h1-3, were isolated from fecal samples of old dog. the analysis of 16s rrna gene sequences indicated that the isolates belonged to the clostridium cluster xvi and were closely related to eubacterium dolichum kctc 5832(t), eubacterium tortuosum dsm 3987(t), clostridium innocuum kctc 5183(t), allobaculum stecoricanis dsm 13633(t), eubacterium limosum kctc 3266(t), and clostridium butyricum kctc 18 ...201728754474
comparative phenotypic analysis of "clostridium neonatale" and clostridium butyricum isolates from neonates."clostridium neonatale" was recently described as a new species within the cluster i of the clostridium genus sensu stricto. in this study, we characterized "c. neonatale" isolates (n = 42) and compared their phenotypic properties with those of clostridium butyricum (n = 26), a close related species. strains isolated from fecal samples of healthy neonates were tested for different phenotypic characteristics. compared to c. butyricum, "c. neonatale" showed a significant higher surface hydrophobic ...201728739338
clostridium butyricum maximizes growth while minimizing enzyme usage and atp production: metabolic flux distribution of a strain cultured in glycerol.the increase in glycerol obtained as a byproduct of biodiesel has encouraged the production of new industrial products, such as 1,3-propanediol (pdo), using biotechnological transformation via bacteria like clostridium butyricum. however, despite the increasing role of clostridium butyricum as a bio-production platform, its metabolism remains poorly modeled.201728571567
[effects of clostridium butyricum on serum uric acid and inflammatory mediators in rats with hyperuricemia].to investigate the effects of intragastric administration of clostridium butyricum in regulating serum uric acid, lipopolysaccharides (lps), interleukin-6 (il-6) and tumor necrosis factor-α (tnf-α) in rats with hyperuricemia rats.201728539294
selection and characterization of an anaerobic microbial consortium with high adaptation to crude glycerol for 1,3-propanediol production.crude glycerol is an ideal feedstock for bioproduction of 1,3-propanediol (1,3-pdo) while pure culture always shows low substrate tolerance and limited productivity. in this study, an anaerobic microbial consortium for conversion of crude glycerol was selected and its 1,3-pdo production capacity was evaluated. the consortium was obtained from anaerobic activated sludge by 19 serial transfers and mainly consisted of 94.64% clostridiaceae and 4.47% peptostreptococcaceae. the consortium adapted wel ...201728512675
clostridium butyricum partially regulates the development of colitis-associated cancer through mir-200c.colitis-associated cancer (cac), one form of colorectal cancer (crc),is an increasing concern worldwide. both diagnosis and current therapy are challenging and bottlenecked. the aim of this study is to investigate novel mechanisms by which the therapeutic c. butyricum regulates colitis-induced oncogenesis. mouse models of cac were established with 2,4,6-trinitrobenzenesulfonic acid (tnbs)and azoxymethane (aom), following by biochemical, clinical and histological analysis. the integrity of epithe ...201728478805
effect of the dietary probiotic clostridium butyricum on growth, intestine antioxidant capacity and resistance to high temperature stress in kuruma shrimp marsupenaeus japonicus.a 56-day feeding trial followed by an acute high temperature stress test were performed to evaluate the effect of dietary probiotic clostridium butyricum (cb) on growth performance and intestine antioxidant capacity of kuruma shrimp marsupenaeus japonicus. shrimp were randomly allocated in 9 tanks (30 shrimp per tank) and triplicate tanks were fed with diets containing different levels of c. butyricum (1×10(9) cfu/g): 0mgg(-1) feed (control), 100mgg(-1) feed (cb-100), 200mgg(-1) feed (cb-200) as ...201728477915
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