complete genome sequences of three phi29-like bacillus cereus group podoviridae.three double-stranded dna phi29-like bacillus cereus group bacteriophages, beachbum, harambe, and serpounce, were identified and characterized. beachbum and harambe are closely related but are remarkably different from previously identified phi29-like phages. serpounce is substantially closer to other phi29-like phages, enabling the identification of its prohead rna (prna) gene.201728729280
an excited state underlies gene regulation of a transcriptional riboswitch.riboswitches control gene expression through ligand-dependent structural rearrangements of the sensing aptamer domain. however, we found that the bacillus cereus fluoride riboswitch aptamer adopts identical tertiary structures in solution with and without ligand. using chemical-exchange saturation transfer (cest) nmr spectroscopy, we revealed that the structured ligand-free aptamer transiently accesses a low-populated (∼1%) and short-lived (∼3 ms) excited conformational state that unravels a con ...201728719589
sinonasal t2r-mediated nitric oxide production in response to bacillus cereus.upper airway epithelial cells produce bactericidal nitric oxide (no) in response to both gram-positive and gram-negative bacteria. our previous work demonstrated that t2r38, a bitter taste receptor (t2r) expressed in airway epithelium, produces no in response to quorum-sensing molecules secreted by pseudomonas aeruginosa. we also demonstrated that staphylococci products elicit an no response when using a t2r-independent pathway. when screening additional human pathogens for epithelial t2r activa ...201728716170
characterization of enterotoxigenic bacillus cereus sensu lato and staphylococcus aureus isolates and associated enterotoxin production dynamics in milk or meat-based broth.bacillus cereus sensu lato species, as well as staphylococcus aureus, are important pathogenic bacteria which can cause foodborne illness through the production of enterotoxins. this study characterised enterotoxin genes of these species and examined growth and enterotoxin production dynamics of isolates when grown in milk or meat-based broth. all b. cereus s. l. isolates harboured nhea, hbla and entfm toxin genes, with lower prevalence of bcet and hlyii. when grown at 16 °c, toxin production by ...201728714887
detection of eight foodborne bacterial pathogens by oligonucleotide array hybridization.simultaneous and rapid detection of multiple foodborne bacterial pathogens is important for the prevention of foodborne illnesses.201728713514
effect of dairy product environment on the growth of bacillus is one of the most important parameters to manage bacterial replication in foodstuffs. in this study, the ability of 2 bacillus cereus strains, 1 clinical human isolate (gpe2) and 1 isolate from a dairy product (d43), were investigated for in vitro growth at different ph values (from 3.5 to 7.5) at 2 temperatures (15 and 37°c), showing their ability to grow from 5.5 to 7.5 and from 5.0 to 7.5, respectively. the ability of spores of these 2 microorganisms to germinate in different typologies o ...201728711259
the potential multiple mechanisms and microbial communities in simultaneous nitrification and denitrification process treating high carbon and nitrogen concentration saline wastewater.a simultaneous nitrification and denitrification (snd) process in sequencing batch biofilm reactor (sbbr) was established to treat high carbon and nitrogen saline wastewater in this study. acetate, glucose and an organic mixture were applied as organic sources in three sbbrs, achieving average total nitrogen removal efficiency of 97.15%, 63.94% and 94.99% during 120days' operation, respectively. the underlying nitrogen removal mechanisms were investigated by 16s rrna sequencing and batch tests. ...201728710998
evaluation of nutrients removal (no3-n, nh3-n and po4-p) with chlorella vulgaris, pseudomonas putida, bacillus cereus and a consortium of these microorganisms in the treatment of wastewater this research removal of nh3-n, no3-n and po4-p nutrients from municipal wastewater was studied, using chlorella vulgaris, pseudomonas putida, bacillus cereus and an artificial consortium of them. the objective is to analyze the performance of these microorganisms and their consortium, which has not been previously studied for nutrient removal in municipal wastewater. a model wastewater was prepared simulating the physicochemical characteristics found at the wastewater plant in chapala, mexic ...201728708609
dynamics of culturable mesophilic bacterial communities of three fresh herbs and their production environment.investigate dynamics of culturable mesophilic bacteria and selected food-contaminating bacteria from three herbs and their production environment.201728708321
molecular ecological network analysis reveals the effects of probiotics and florfenicol on intestinal microbiota homeostasis: an example of sea cucumber.animal gut harbors diverse microbes that play crucial roles in the nutrition uptake, metabolism, and the regulation of host immune responses. the intestinal microbiota homeostasis is critical for health but poorly understood. probiotics paracoccus marcusii db11 and bacillus cereus g19, and antibiotics florfenicol did not significantly impact species richness and the diversity of intestinal microbiota of sea cucumber, in comparison with those in the control group by high-throughput sequencing. mo ...201728684750
analysis of culturable and non-culturable bacteria and their potential to form biofilms in a primary treated dairy wastewater system.biofilm formation in a dairy waste irrigation system can reduce treatment capacity and increase maintenance and cleaning. an extreme biofilm observed in a primary treated dairy wastewater system blocked the irrigation line requiring manual cleaning. both next-generation genomic sequencing and the culturable fraction showed the presence of predominantly gram-negative bacteria. isolates identified from current samples and stored samples from the extreme biofilm included pseudomonas, citrobacter, k ...201728681658
implications of neuroinvasive bacterial peptides on rodents behaviour and neurotransmission.neuroinvasive microbes are capable of applying their influences on the autonomic nervous system (ans) of the host followed by the involvement of central nervous system (cns) by releasing extracellular metabolites that may cause alterations in the biochemical and neurophysiological environment. consequently synaptic, neuroendocrine, peripheral immune, neuro-immune, and behavioural responses of the host facilitate the progression of infection. the present study was designed to extrapolate the effe ...201728671588
resolution of enantiopure (s)-1-(1-napthyl) ethanol from racemic mixture by a novel bacillus cereus isolate.chiral intermediates have wide application and high demand in pharmaceutical, agricultural, and other biotechnological industries for the preparation of bulk drug substances or fine chemicals. (s)-1-(1-napthyl) ethanol is an important synthetic intermediate of mevinic acid analog and a potential inhibitor of 3-hydroxy methyl glutaryl coenzyme a reductase enzymes which is rate limiting for cholesterol synthesis. the present study focuses on the resolution of (rs)-1-(1-napthyl) ethanol using whole ...201728671310
salicylaldehyde and dihydroisobenzofuran derivatives from the marine fungus zopfiella marina.two salicylaldehyde derivatives (1 and 2), a hydroxymethylphenol (3), five dihydroisobenzofuran (4-8) derivatives, and a 5-chloro-3-deoxyisoochracinic acid (9), together with a known 3-deoxyisoochracinic acid (10) were isolated from the marine fungus zopfiella marina bcc 18240 (or nbrc 30420). the structures of these compounds were elucidated by extensive spectroscopic analysis. compound 1 showed weak antituberculous activity against mycobacterium tuberculosis h37ra, and antibacterial activity a ...201728669223
structure and functional characterisation of a distinctive β-lactamase from an environmental strain emb20 of bacillus cereus.the rampant use and misuse of antibiotics in human medicine, agriculture and veterinary have become the key contributors to global antimicrobial resistance. one of the significant resistance mechanisms that inactivates antibiotics and impedes treatment of bacterial infections is the expression of β-lactamases. rising evidence of newer variants of β-lactamases in the environment is therefore a serious threat to the presently available antibiotic armoury. the present work describes the purificatio ...201728664525
draft genome sequence of pseudomonas koreensis ci12, a bacillus cereus "hitchhiker" from the soybean rhizosphere.pseudomonas koreensis ci12 was coisolated with bacillus cereus from a root of a soybean plant grown in a field in arlington, wi. here, we report the draft genome sequence of p. koreensis ci12 obtained by illumina sequencing.201728663293
the antibacterial activity and modes of li-f type antimicrobial peptides against bacillus cereus in are a family of highly potent cyclic lipodepsipeptide antibiotics with a broad antimicrobial spectrum (gram-positive bacteria and fungi). in this study, li-f-type antimicrobial peptides (amp-jsa9) composing of li-f03a, li-f03b, li-f04a, li-f04b and li-f05b were isolated from paenibacillus polymyxa jsa-9. to better understand the antimicrobial mechanism of amp-jsa9, the potency and action(s) of amp-jsa9 against bacillus cereus were examined.201728650559
isolation, selection, and biological characterization research of highly effective electricigens from mfcs for phenol degradation.the microbial fuel cells (mfcs) are recognized to be highly effective for the biodegradation of phenol. for isolating the phenol-degrading bacteria, the sample containing 500 mg/l phenol was collected from the mfcs. the strain (wl027) was identified basing on the 16s rrna gene analysis and phylogenetic analysis as bacillus cereus. the effects of ph, temperature, concentrations of phenol, heavy metal ions, and salt on the growth of strain as well as the degradation of phenol have been carefully s ...201728646380
enhanced antibacterial activity of ent-labdane derivatives of salvic acid (7α-hydroxy-8(17)-ent-labden-15-oic acid): effect of lipophilicity and the hydrogen bonding role in bacterial membrane the present study, the antibacterial activity of several ent-labdane derivatives of salvic acid (7α-hydroxy-8(17)-ent-labden-15-oic acid) was evaluated in vitro against the gram-negative bacterium escherichia coli and the gram-positive bacteria staphylococcus aureus and bacillus cereus. for all of the compounds, the antibacterial activity was expressed as the minimum inhibitory concentration (mic) in liquid media and minimum inhibitory amount (mia) in solid media. structure activity relations ...201728644410
inactivation of bacillus cereus spores on red chili peppers using a combined treatment of aqueous chlorine dioxide and hot-air drying.the effect of a combined treatment using aqueous chlorine dioxide (clo2 ) and hot-air drying to inactivate bacillus cereus spores on red chili peppers was evaluated. ten washed and dried pepper samples, each comprising half of a single pepper (capsicum annuum l.), were inoculated with b. cereus spore suspension. the inoculated samples were washed with sodium hypochlorite (naocl; 50, 100, or 200 μg/ml) or clo2 (50, 100, or 200 μg/ml) solution for 1 min and then air-dried (25 ± 1 °c, 47 ± 1% relat ...201728631818
high level bacterial contamination of secondary school students' mobile phones.while contamination of mobile phones in the hospital has been found to be common in several studies, little information about bacterial abundance on phones used in the community is available. our aim was to quantitatively determine the bacterial contamination of secondary school students' mobile phones.201728626737
rapid, high-throughput identification of anthrax-causing and emetic bacillus cereus group genome assemblies using btyper, a computational tool for virulence-based classification of bacillus cereus group isolates using nucleotide sequencing data.the bacillus cereus group comprises nine species, several of which are pathogenic. differentiating between isolates that may cause disease and those that do not is a matter of public health and economic importance, but can be particularly challenging due to the high genomic similarity of the group. to this end, we have developed btyper, a computational tool that employs a combination of (i) virulence gene-based typing, (ii) multi-locus sequence typing (mlst), (iii) panc clade typing, and (iv) rp ...201728625989
isolation, morphological identification and in vitro antibacterial activity of endophytic bacteria isolated from azadirachta indica (neem) leaves.the objective of this study was to isolate endophytic bacteria from azadirachta indica (neem) leaves, their identification and investigate their antibacterial activity against three gram-positive bacteria, staphylococcus aureus, streptococcus pyogenes and bacillus cereus and gram-negative bacteria escherichia coli, salmonella typhimurium and klebsiella pneumoniae.201728620254
nmr structure of the bacillus cereus hemolysin ii c-terminal domain reveals a novel addition to multiple virulence factors, bacillus cereus a pathogen that causes food poisoning and life-threatening wound infections, secretes the pore-forming toxin hemolysin ii (hlyii). the hlyii toxin has a unique 94 amino acid c-terminal domain (hlyiic). hlyiic exhibits splitting of nmr resonances due to cis/trans isomerization of a single proline near the c-terminus. to overcome heterogeneity, we solved the structure of p405m-hlyiic, a mutant that exclusively stabilizes the trans state. t ...201728607368
isolation and preliminary characterization of a bacteriocin-producer bacillus strain inhibiting methicillin resistant staphylococcus a multivalent approach to discover new antimicrobial substance, a total of 160 bacilli were isolated from termitarium soil, characterized on the basis of their morphological and physiological characters and screened for their antimicrobial activity by agar well diffusion method against certain drug resistant pathogenic bacteria such as staphylococcus aureus, methicillin resistant staphylococcus aureus and common food contaminating bacteria listeria monocytogenes. after preliminary screening, ...201728605978
characterization of hypericum perforatum polysaccharides with antioxidant and antimicrobial activities: optimization based statistical this study, the extracting parameters of crude polysaccharides (cpss) from the hypericum perforatum (hp) including extraction time (eti, 60-180min), extraction temperature (ete, 60-90°c), and the water/solid ratio (w/s, 10-30 was optimized by using three-variable-three-level box-behnken design-response surface methodology (bbd-rsm) based on the single-factor experiments. the optimal extraction conditions were as follow: eti 117.5min, ete 74.28°c, and w/s 20.3:1. under these conditions, the ex ...201728602988
an enhancement of antimicrobial efficacy of biogenic and ceftriaxone-conjugated silver nanoparticles: green approach.of the various methods explored for the synthesis of nanoparticles, biogenesis of silver nanoparticles (agnps) received great attention due to their versatile properties. in this report, daucus carota extract was used for the synthesis of agnps and ceftriaxone was conjugated with agnps to enhance their antimicrobial efficacy. the conjugated and unconjugated agnps were characterized by adopting uv-vis spectroscopy, ftir, afm, dls, and tem, which revealed the spr peak at 420 nm and spherical shape ...201728600792
do multi-year applications of bacillus thuringiensis subsp. israelensis for control of mosquito larvae affect the abundance of b. cereus group populations in riparian wetland soils?bacillus thuringiensis subsp. israelensis (bti) is a soil-borne bacterium affiliated to the bacillus cereus group (bcg) and has been used in biocontrol products against nematoceran larvae for several decades. however, knowledge is limited on whether long-term bti application can affect the structure of indigenous communities of bcg and the overall abundance of bti. using species- and group-specific quantitative pcr assays, we measured the bcg- and bti-abundances in riparian wetlands in the river ...201728600590
a purely green synthesis of silver nanoparticles using carica papaya, manihot esculenta, and morinda citrifolia: synthesis and antibacterial procedure for synthesizing silver nanoparticles (agnps) is currently considered due to its economy and toxic-free effects. several existing works on synthesizing agnps using leaves extract still involve the use of physical or mechanical treatment such as heating or stirring, which consume a lot of energy. to extend and explore the green extraction philosophy, we report here the synthesis and antibacterial evaluations of a purely green procedure to synthesize agnps using carica papaya, mani ...201728597212
risk factors of catheter-related bloodstream infection caused by bacillus cereus: case-control study in 8 teaching hospitals in this multicenter, matched case-control study, patients diagnosed with catheter-related bloodstream infection (crbsi) caused by bacillus cereus (n = 108) were matched to controls (n = 269). in the multivariable analysis, administration of an amino acid preparation and an indwelling peripheral catheter were significant variables for b cereus-related crbsi.201728596019
a novel technique for sterilization using a power self-regulated single-mode microwave this paper, a novel technique to achieve precise temperatures in food sterilization has been proposed. an accurate temperature profile is needed in order to reach a commitment between the total removal of pathogens inside the product and the preservation of nutritional and organoleptic characteristics. the minimal variation of the target temperature in the sample by means of a monitoring and control software platform, allowing temperature stabilization over 100 °c, is the main goal of this wo ...201728590423
evaluation of cytotoxicity of hematite nanoparticles in bacteria and human cell lines.metal nanoparticles have a great impact and even change the composition of soil microbial communities. this poses the risk of their accumulation in the ecosystem, which may call on health hazard. statistical techniques such as plackett-burman design, response surface methodology were used for optimizing medium constituents for bacillus cereus svk1 and other critical variables responsible for the production of biomolecules and biosynthesis of hematite nanoparticles. the effect of hematite nanopar ...201728586726
phosphate crosslinked pectin based dual responsive hydrogel networks and nanocomposites: development, swelling dynamics and drug release characteristics.potential dual responsive hydrogel networks (ppad) are fabricated from pectin, poly((2-dimethylamino)ethyl methacrylate)) and phosphate crosslinker bis[2-methacryloyloxy] ethyl phosphate (bmep) by a simple free radical polymerization. these hydrogel networks are successfully utilized for encapsulation of an anti-cancer drug, 5-fluorouracil (5-fu) and also employed as versatile platforms for production of silver nanoparticles. fabricated hydrogel networks and silver nanocomposites were characteri ...201728576553
bacterial contaminants from frozen puff pastry production process and their growth inhibition by antimicrobial substances from lactic acid bacteria.seventy-five bacterial contaminants which still persisted to cleaning system from three puff pastry production lines (dough forming, layer and filling forming, and shock freezing) were identified using 16s rdna as seven genera of bacillus, corynebacterium, dermacoccus, enterobacter, klebsiella, pseudomonas, and staphylococcus with detection frequencies of 24.00, 2.66, 1.33, 37.33, 1.33, 2.66, and 30.66, respectively. seventeen species were discovered while only 11 species bacillus cereus, b. sub ...201728572930
molecular and functional characterization of myxobacteria isolated from soil in india.this study reports the isolation of myxobacteria from soil collected from plains in north india. based on the morphology and 16s rdna sequence, the isolated myxobacteria were identified as corallococcus sp., pyxidicoccus sp., myxococcus sp., cystobacter sp. and archangium sp. the myxobacteria were functionally characterized to assess their ability to produce antibacterial and anticancer metabolites. the isolates were found to be functionally versatile as they produced extracellular bioactive mol ...201728567623
a study to assess the numbers and prevalence of bacillus cereus and its toxins in pasteurized fluid milk.bacillus cereus is a pathogenic adulterant of raw milk and can persist as spores and grow in pasteurized milk. the objective of this study was to determine the prevalence of b. cereus and its enterotoxins in pasteurized milk at its best-before date when stored at 4, 7, and 10°c. more than 5.5% of moderately temperature-abused products (stored at 7°c) were found to contain >10(5) cfu/ml b. cereus , and about 4% of them contained enterotoxins at a level that may result in foodborne illness; in add ...201728561637
molecular characterization and risk assessment of bacillus cereus sensu lato isolated from ultrahigh-temperature and pasteurized milk marketed in rio de janeiro, brazil.the presence of bacillus cereus in milk is a major concern in the dairy industry. in this study 27 bacillus cereus sensu lato isolates from pasteurized and ultrahigh-temperature (uht) milk (24 whole uht and 4 pasteurized samples) collected at supermarket chains in rio de janeiro, brazil, were evaluated to assess the potential risk for food poisoning. toxigenic and virulence profiles were defined by gene-specific pcr. affiliation to phylogenetic groups was assigned by panc sequencing. microbiolog ...201728556679
heteroleptic metal(ii) complexes of hydrotris(methimazolyl)borate and diimines: synthesis, theoretical calculations, antimicrobial, antioxidant, in vitro cytotoxicity and molecular docking studies.a series of heteroleptic metal(ii) complexes of formulation [m(tm)(diimine)](clo4) (1-8), [tm = hydrotris(methimazolyl)borate, diimine = 2,2'-bipyridyl or 1,10-phenanthroline and m = mn(ii), ni(ii), cu(ii) or zn(ii)] have been synthesized and characterized by spectroscopic methods. the geometric parameters of the complexes were determined using uv-vis spectroscopy and dft calculations. the analyses of homo and lumo have been used to explain the charge transfer within the molecule. antimicrobial ...201728546116
identification of microorganisms isolated from counterfeit and unapproved decorative contact lenses.all contact lenses (corrective/noncorrective) are considered class ii or class iii medical devices under the federal food, drug, and cosmetic act, which also states that contact lenses can only be obtained with a prescription. the forensic chemistry center of the us food & drug administration has examined over 300 decorative, noncorrective contact lenses obtained without a prescription. our observations indicate that 60% of the counterfeit lenses and 27% of the unapproved lenses examined were po ...201728542816
antibacterial cysteine protease from cissus quadrangularis antibacterial cp was extracted from the stem of cissus quadrangularis and purified with a 5.39 fold increase in specific activity and 8.67% recovery. the molecular weight of the purified enzyme was estimated to be 39kda by sds-page. the purified enzyme appeared as a single band on native-page. the optimum ph and temperature for protease activity were around 6.0 and 50°c respectively. the cp showed ph stability from 3 to 10 and retained more than 90% of its relative protease activity. the addi ...201728539266
bacillus cereus-type polyhydroxyalkanoate biosynthetic gene cluster contains r-specific enoyl-coa hydratase gene.bacillus cereus and bacillus megaterium both accumulate polyhydroxyalkanoate (pha) but their pha biosynthetic gene (pha) clusters that code for proteins involved in pha biosynthesis are different. namely, a gene encoding maoc-like protein exists in the b. cereus-type pha cluster but not in the b. megaterium-type pha cluster. maoc-like protein has an r-specific enoyl-coa hydratase (r-hydratase) activity and is referred to as phaj when involved in pha metabolism. in this study, the pha cluster of ...201728532241
interaction of nanostructured tio2 biointerfaces with stem cells and biofilm-forming bacteria.nanostructured tio2 nanotubes (nts) of diameters from 15 to 100nm were fabricated by an electrochemical anodization process. biofilm-positive strains of bacillus cereus and pseudomonas aeruginosa behaved similarly on all tio2 nts as well as on native titanium (ti) foil. the adhesion and growth of mesenchymal stem cells (msc), embryonic stem cells (esc), and pure cardiomyocytes derived from esc exhibited significant differences. msc as well as esc were, in contrast to cardiomyocytes, able to adhe ...201728532058
syntrophic association of termite gut bacterial symbionts with bifunctional characteristics of cellulose degrading and polyhydroxyalkanoate producing bacteria.the guild between higher termites and their partnership with the diverse community of bacteria and archaea in their gut is a marvel evolutionary achievement. sustained attempts were made worldwide with a quest for identifying viable important biological macromolecule polyhydroxyalkanoate (pha) accumulating bacteria. termite gut serve as a novel source for bacteria with dual properties like pha production as well as cellulose degradation. among 40 isolates cultivated, 32.5% turned positive for pc ...201728528947
revealing the ability of a novel polysaccharide bioflocculant in bioremediation of heavy metals sensed in a vibrio bioluminescence reporter assay.a bioflocculant-producing bacterial strain, designated msi021, was isolated from the marine sponge dendrilla nigra and demonstrated 94% flocculation activity in a kaolin clay suspension. msi021 was identified as bacillus cereus based on phylogenetic affiliation and biochemical characteristics. the purified extra-cellular bioflocculant was chemically elucidated as a polysaccharide molecule. the polysaccharide bioflocculant was stable under both acidic and alkaline conditions (ph 2.0-10.0) and tem ...201728527323
diversity and probiotic potential of lactic acid bacteria isolated from horreh, a traditional iranian fermented food.the aim of this study was to evaluate the probiotic potential of lactic acid bacteria (lab) strains isolated from horreh. some probiotic properties, e.g., resistance to acid, bile tolerance, antibacterial activity, and antibiotic susceptibility, were investigated. a total of 140 gram-positive and catalase-negative isolates from horreh were subjected to identification and grouping by cultural methods and the 16s rrna sequencing. the new isolates were identified to be lactobacillus (fermentum, pla ...201728527125
release study and inhibitory activity of thyme essential oil-loaded chitosan nanoparticles and nanocapsules against foodborne bacteria.the antibacterial property of thyme essential oil due to different volatile compounds, has been well documented in the literature. to overcome the high volatility of essential oil components, encapsulation has emerged as a new alternative. in this work, chitosan and thyme essential oil-loaded chitosan nanoparticles (teo-csnps) and nanocapsules (teo-csncs) were prepared by nanoprecipitation and nanoencapsulation, respectively. the morphology, encapsulation efficiency, release kinetics, and inhibi ...201728526346
non-contact electromagnetic induction heating for eradicating bacteria and yeasts on biomaterials and possible relevance to orthopaedic implant infections: in vitro findings.infection of implants is a major problem in elective and trauma surgery. heating is an effective way to reduce the bacterial load in food preparation, and studies on hyperthermia treatment for cancer have shown that it is possible to heat metal objects with pulsed electromagnetic fields selectively (pemf), also known as induction heating. we therefore set out to answer the following research question: is non-contact induction heating of metallic implants effective in reducing bacterial load in v ...201728522446
spotlight on bacillus cereus and its food poisoning toxins. 201728521335
decontamination of multiple heavy metals-containing effluents through microbial decontaminate heavy metal-containing waste water, a microbial biotechnology was developed by using the synergy between sulfate reducing bacteria (srb), bacillus cereus (b. cereus) and camellia oleifera cake (coc). in this process the coc degradation assisted by b.cereus, created an anoxic environment and provided energy and nutrition for srb. both of b. cereus and srb played significant roles through biosorption, bioaccumulation and biosurfactant production. meanwhile, a flotation technology ...201728521206
cyclic diarylheptanoids from corylus avellana green leafy covers: determination of their absolute configurations and evaluation of their antioxidant and antimicrobial activities.the methanol extract of the leafy covers of corylus avellana, source of the italian pgi (protected geographical indication) product "nocciola di giffoni", afforded two new cyclic diarylheptanoids, giffonins t and u (2 and 3), along with two known cyclic diarylheptanoids, a quinic acid, flavonoid-, and citric acid derivatives. the structures of giffonins t and u were determined as highly hydroxylated cyclic diarylheptanoids by 1d and 2d nmr experiments. their relative configurations were assigned ...201728520428
comparative genomic analysis reveals genetic features related to the virulence of bacillus cereus forc_013.bacillus cereus is well known as a gastrointestinal pathogen that causes food-borne illness. in the present study, we sequenced the complete genome of b. cereus forc_013 isolated from fried eel in south korea. to extend our understanding of the genomic characteristics of forc_013, we conducted a comparative analysis with the published genomes of other b. cereus strains.201728515790
former food products safety: microbiological quality and computer vision evaluation of packaging remnants contamination.the use of alternative feed ingredients in farm animal's diets can be an interesting choice from several standpoints, including safety. in this respect, this study investigated the safety features of selected former food products (ffps) intended for animal nutrition produced in the framework of the izs plv 06/14 rc project by an ffp processing plant. six ffp samples, both mash and pelleted, were analysed for the enumeration of total viable count (tvc) (iso 4833), enterobacteriaceae (iso 21528-1) ...201728513297
antibacterial and antioxidative activity of o-amine functionalized chitosan.cinnamaldehyde was immobilized to o-amine functionalized chitosan via a coupling reaction. fourier transform infrared spectroscopy confirmed n-cinnamyl substitution. wetting analyses demonstrate more hydrophobicity in the n-cinnamyl substituted o-amine functionalized chitosan compared to chitosan or unsubstituted o-amine functionalized chitosan. thermal gravimetric analysis and differential scanning calorimetry demonstrates that the prepared n-cinnamyl substituted o-amine functionalized chitosan ...201728504167
effect of glucose and olive oil as potential carbon sources on production of phas copolymer and tercopolymer by bacillus cereus this study, the influence of different physicochemical parameters on the yield of polyhydroxyalkanoates (phas) produced by bacillus cereus fa11 is investigated. the physicochemical factors include ph, temperature, time, inoculum size and its age, agitation speed and composition of the glucose rich peptone deficient (grpd) medium. during two-stage fermentation, b. cereus fa11 produced a significantly high (p < 0.05) yield (80.59% w/w) of phas copolymer using grpd medium containing glucose (15  ...201728501987
lateral flow assay-based bacterial detection using engineered cell wall binding domains of a phage endolysin.the development of a cost-effective and efficient bacterial detection assay is essential for diagnostic fields, particularly in resource-poor settings. although antibodies have been widely used for bacterial capture, the production of soluble antibodies is still expensive and time-consuming. here, we developed a nitrocellulose-based lateral flow assay using cell wall binding domains (cbds) from phage as a recognition element and colloidal gold nanoparticles as a colorimetric signal for the detec ...201728494369
soil acidification aggravates the occurrence of bacterial wilt in south china.soil acidification is a major problem in modern agricultural systems and is an important factor affecting the soil microbial community and soil health. however, little is known about the effect of soil acidification on soil-borne plant diseases. we performed a 4-year investigation in south china to evaluate the correlation between soil acidification and the occurrence of bacterial wilt. the results showed that the average soil ph in fields infected by bacterial wilt disease was much lower than t ...201728487678
antimicrobial activity of aqueous, ethanolic and methanolic leaf extracts from acacia spp. and eucalyptus europe, acacia and eucalyptus, originate large amounts of biomass, due to their need by industries and other biological control, that can be used to extract antimicrobial substances.201628480369
aptamer-based trapping: enrichment of bacillus cereus spores for real-time pcr detection.aptamer-based trapping techniques are in general suitable to replace common antibody-based enrichment approaches. a time-consuming isolation or clean-up is often necessary during sample preparation, e.g. for the detection of spores. for the development of bioanalytical routine approaches, aptamers with a high affinity to b. cereus spores were applied for the establishment and validation of an aptamer-based trapping technique in milk with fat contents between 0.3 and 3.5%. thereby, enrichment fac ...201728478557
the phosphotransferase system gene ptsi in bacillus cereus regulates expression of soda2 and contributes to colonization of wheat roots.plant growth-promoting rhizobacteria effectively enhance plant growth and root colonization by the bacteria is a prerequisite during the process. bacillus cereus 905, a rhizosphere bacterium originally isolated from wheat roots, colonizes the wheat rhizosphere with a large population size. we previously showed that a manganese-containing superoxide dismutase (mnsod2), encoded by the soda2 gene, plays an important role in colonization of the wheat rhizosphere by b. cereus 905. in this study, we i ...201728478075
green synthesis, characterisation and bioactivity of plant-mediated silver nanoparticles using decalepis hamiltonii root extract.consistent search of plants for green synthesis of silver nanoparticles (snps) is an important arena in nanomedicine. this study focuses on synthesis of snps using bioreduction of silver nitrate (agno3) by aqueous root extract of decalepis hamiltonii. the biosynthesis of snps was monitored by uv-vis analysis at absorbance maxima 432 nm. the fluorescence emission spectra of snps illustrated the broad emission peak 450-483 nm at different excitation wavelengths. the surface characteristics were st ...201728476981
seasonal outbreak of bacillus bacteremia associated with contaminated linen in hong kong.a high seasonal incidence of bacillus bacteremia was associated with the use of contaminated hospital linens.201728475782
predicting genome terminus sequences of bacillus cereus-group bacteriophage using next generation sequencing data.most tailed bacteriophages (phages) feature linear dsdna genomes. characterizing novel phages requires an understanding of complete genome sequences, including the definition of genome physical ends.201728472946
the putative drug efflux systems of the bacillus cereus group.the bacillus cereus group of bacteria includes seven closely related species, three of which, b. anthracis, b. cereus and b. thuringiensis, are pathogens of humans, animals and/or insects. preliminary investigations into the transport capabilities of different bacterial lineages suggested that genes encoding putative efflux systems were unusually abundant in the b. cereus group compared to other bacteria. to explore the drug efflux potential of the b. cereus group all putative efflux systems wer ...201728472044
evaluation of two standard and two chromogenic selective media for optimal growth and enumeration of isolates of 16 unique bacillus species.the genus bacillus is a group of gram-positive endospore-forming bacteria that can cause food poisoning and diarrheal illness in humans. a wide range of food products have been linked to foodborne outbreaks associated with these opportunistic pathogens. the u.s. food and drug administration recommends (in their bacteriological analytical manual) the use of bacara or mannitol egg yolk polymyxin (myp) agar plates and the most-probable-number (mpn) method for enumeration and confirmation of bacillu ...201728467187
self assembled snowball-like hybrid nanostructures comprising viburnum opulus l. extract and metal ions for antimicrobial and catalytic applications.herein, we report the production of novel organic-inorganic nanobio-catalytic and antimicrobial agents called "nanosnowball" (nsbs) with a rational design and elucidate the increase in the catalytic and antimicrobial activities. the nsbs resemble to guelder rose were formed of the extract of viburnum opulus (vo) as the organic component and copper (ii) ions (cu(2+)) as the inorganic component. the effects of the vo extract and cu(2+) concentrations on the morphology of nsbs were systematically e ...201728465062
paneth-cell-disruption-induced necrotizing enterocolitis in mice requires live bacteria and occurs independently of tlr4 signaling.necrotizing enterocolitis (nec) remains a leading cause of morbidity and mortality in premature infants. both human surgical specimens and animal models suggest a potential involvement of paneth cells in nec pathogenesis. paneth cells play critical roles in epithelial homeostasis, innate immunity and host-microbial interactions. yet, the complex interplay between paneth cell disruption, epithelial barrier dysfunction and microbial-driven inflammation remains unclear in the immature intestine. in ...201728450472
simulating intestinal growth conditions enhances toxin production of enteropathogenic bacillus cereus.bacillus cereus is a ubiquitous bacterial pathogen increasingly reported to be the causative agent of foodborne infections and intoxications. since the enterotoxins linked to the diarrheal form of food poising are foremost produced in the human intestine, the toxic potential of enteropathogenic b. cereus strains is difficult to predict from studies carried out under routine cultivation procedures. in this study, toxigenic properties of a panel of strains (n = 19) of diverse origin were compared ...201728446903
bacteriophage exclusion (brex): a novel anti-phage mechanism in the arsenal of bacterial defense system.the arm race between bacteria and phages leads to the development of many phage resistance mechanisms. bacteria are perpetually changing their strategy to avert phage infection and killing. recently, a study published that shed light on a novel bacterial defense system called bacteriophage exclusion (brex). brex system is a six-gene cassette in bacillus cereus which provides complete phage resistance to a broad range of phages, including lytic and temperate ones. it is a novel defense strategy w ...201728444888
surveillance of bacillus cereus isolates in korea from 2012 to investigate the prevalence and toxin production characteristics of non-emetic and emetic bacillus cereus strains isolated via the laboratory surveillance system in korea.201728443227
indole derivatives produced by the metagenome genes of the escherichia coli-harboring marine sponge discodermia calyx.three indole derivatives, a novel benzoxazine-indole hybrid (1) and two known indole trimers (2, 3), were isolated from the metagenomic library of the marine sponge discodermia calyx based on functional screening. their structures were elucidated by extensive spectroscopic analysis and comparison of their nmr data to that of known compounds. the antibacterial assay indicated that only compound 2 displayed significant antibacterial activity against bacillus cereus, with approximately 20 mm diamet ...201728441364
genome-wide investigation of biofilm formation in bacillus cereus.bacillus cereus is a soil-dwelling gram-positive bacterium capable of forming structured multicellular communities, or biofilms. however, the regulatory pathways controlling biofilm formation are less well understood in b. cereus in this work, we developed a method to study b. cereus biofilms formed at the air-liquid interface. we applied two genome-wide approaches, random transposon insertion mutagenesis to identify genes that are potentially important for biofilm formation, and transcriptome a ...201728432092
the spo0a-sini-sinr regulatory circuit plays an essential role in biofilm formation, nematicidal activities, and plant protection in bacillus cereus ar156.the rhizosphere bacterium bacillus cereus ar156 is capable of forming biofilms, killing nematodes, and protecting plants. however, the underlying molecular mechanisms of these processes are not well understood. in this study, we found that the isogenic mutants δbcspo0a and δbcsini have significantly reduced colonization and nematicidal activity in vitro and biological control efficacy on the tomato plant under greenhouse conditions. we further investigated the role of the spo0a-sini-sinr regulat ...201728430084
epidemiological study of common diseases and their risk factors in camels in south punjab, pakistan.bacteriological study of mastitis along with common blood protozoan diseases were studied in dromedary camels in cholistan, dera ismail khan and rahim yar khan districts in south punjab, pakistan. for this purpose 300 camels were sampled randomly at different common grazing and watering point. for study of blood parasites clinically suspected and apparently healthy camels, 150 each, were sampled. an overall prevalence of 15%and 5% was recorded for trypanosomiasis and anaplasmosis respectively. t ...201728428133
infection-related complications during treatment for childhood acute lymphoblastic leukemia.comprehensive studies on neutropenia and infection-related complications in patients with acute lymphoblastic leukemia (all) are lacking.201728426102
effective thermal inactivation of the spores of bacillus cereus biofilms using microwave.microwave sterilization was performed to inactivate the spores of biofilms of bacillus cereus involved in foodborne illness. the sterilization conditions, such as the amount of water and the operating temperature and treatment time, were optimized using statistical analysis based on 15 runs of experimental results designed by the box-behnken method. statistical analysis showed that the optimal conditions for the inactivation of b. cereus biofilms were 14 ml of water, 108°c of temperature, and 15 ...201728423891
effects of culture conditions on the size, morphology and wet density of spores of bacillus cereus 569 and bacillus megaterium qm b1551.the influence of variable culture conditions on the size and wet density of spores of bacillus cereus and bacillus megaterium were examined in this work. culture temperature and initial ph was shown to have a significant impact on the size of both species, with increasingly alkaline culture media and elevated culture temperatures resulting in spores that were, on average, up to 25% reduced in volume. increasing concentrations of inorganic salts in sporulation media exerted differing effects on e ...201728419596
virulence analysis of bacillus cereus isolated after death of preterm neonates, nice, france, 2013.after the deaths of 2 preterm neonates with bacillus cereus systemic infection in the same intensive care unit, we investigated the pathogenic potential of this bacterium. genetic and virulence analysis indicated the neonates were infected with 2 different strains with a virulence potential similar to environmental strains, indicating likely patient immune response failure.201728418291
crystal structure of the flagellar chaperone flis from bacillus cereus and an invariant proline critical for flis dimerization and flagellin recognition.flis is a cytoplasmic flagellar chaperone for the flagellin, which polymerizes into filaments outside of the flagellated bacteria. cytoplasmic interaction between flis and flagellin is critical to retain the flagellin protein in a monomeric form, which is transported from the cytoplasm through the flagellar export apparatus to the extracellular space for filament assembly. defects in the flis protein directly diminish bacterial motility, pathogenicity, and viability. although the overall structu ...201728414127
production and characterization of curcumin microcrystals and evaluation of the antimicrobial and sensory aspects in minimally processed carrots.nontoxic conserving agents are in demand by the food industry due to consumers concern about synthetic conservatives, especially in minimally processed food. the antimicrobial activity of curcumin, a natural phenolic compound, has been extensively investigated but hydrophobicity is an issue when applying curcumin to foodstuff. the objective of this work was to evaluate curcumin microcrystals as an antimicrobial agent in minimally processed carrots. the antimicrobial activity of curcumin microcry ...201728406506
ribosomal background of the bacillus cereus group this study we reconstructed the architecture of bacillus cereus sensu lato population based on ribosomal proteins, and identified a link between the ribosomal proteins' variants and thermal groups (thermotypes) of the bacilli. the in silico phyloproteomic analysis of 55 ribosomal proteins (34 large and 21 small subunit r-proteins) of 421 strains, representing 14 well-established or plausible b. cereus sensu lato species, revealed several ribosomal clusters (r-clusters), which in general were ...201728406161
standardization of biopolymer production from seaweed associative bacteria.polyhydroxyalkanoate (pha) is the biodegradable plastic, which is shown the similar properties to the synthetic plastic. production of pha is expensive when compared with petrochemical based plastics costs and also hazardous to ecosystem. in order to overcome these drawbacks, some cheaper commercially available carbon sources like rice bran, coconut cake, palm jaggary, etc. can be used. the present study aimed to identify the potential pha producing bacterial strains from different seaweeds. bas ...201728404223
cereulide production by bacillus weihenstephanensis strains during growth at different ph values and temperatures.besides bacillus cereus, some strains of the psychrotolerant, potentially foodborne pathogen bacillus weihenstephanensis can produce the emetic toxine (cereulide). this toxin is a heat- and acid-stable cyclic dodecadepsipeptide that causes food intoxication with vomiting. however, some severe clinical cases with lethal outcomes have been described. if cereulide can be produced during refrigerated storage, it will not be inactivated by reheating food, representing an important risk of food intoxi ...201728399995
analysis of kudoa septempunctata as a cause of foodborne illness and its associated differential diagnosis.recently kudoa septempuctata in olive flounders is suggested as a cause of food poisoning, however whether kudoa septempuctata can affect human gastrointestinal systems is controversial and its pathogenecity remains unclear. in view of the field epidemiology, food poisonings caused by kudoa septempuctata should be distinguished from those caused by staphylococcus aureus and bacillus cereus.201728395399
antibacterial and antibiotic potentiating activities of tropical marine sponge extracts.increasing prevalence of antibiotic resistance has led research to focus on discovering new antimicrobial agents derived from the marine biome. although ample studies have investigated sponges for their bioactive metabolites with promising prospects in drug discovery, the potentiating effects of sponge extracts on antibiotics still remains to be expounded. the present study aimed to investigate the antibacterial capacity of seven tropical sponges collected from mauritian waters and their modulat ...201728392375
genetic diversity and population structure of the bacillus cereus group bacteria from diverse marine environments.the phylogenetic diversity of marine bacteria belonged to the bacillus cereus group has not been well investigated. here, we present the genetic diversity and population structure of 71 bacteria from diverse marine environments, using a multilocus sequence typing (mlst) approach and the analyses of digital dna-dna hybridization (dddh) and average nucleotide identity (ani) based on some representative genomic sequences. the mlst analysis demonstrated that these isolates were highly diverse and a ...201728386130
relapsing bacillus cereus peritonitis in a patient treated with continuous ambulatory peritoneal dialysis.peritonitis is a severe complication of peritoneal dialysis (pd) due to associated morbidity and mortality. although bacillus cereus is mostly considered as a contaminant, its role as a causative agent in a few cases of pd peritonitis has been documented. peritonitis due to b. cereus has been associated with high rates of catheter removal and resistance to beta-lactam antibiotics.201428663812
evaluation of in vitro cytotoxic activity of mono-pegylated stap3 (solanum tuberosum aspartic protease 3) forms.stap3 is a plant aspartic protease with cytotoxic activity toward a broad spectrum of pathogens, including potato and human pathogen microorganisms, and cancer cells, but not against human t cells, human red blood cells or plant cells. for this reason, stap3 could be a promising and potential drug candidate for future therapies. in this work, the improvement of the performance of stap3 was achieved by means of a modification with peg. the separation of a mono-pegylated stap3 fraction was easily ...201428626641
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