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preparation and characterization of immobilized beta-lactamase for destruction of penicillin in milk.beta-lactamase i (bacillus cereus) was covalently bound to cyanogen bromide-activated, crosslinked agarose. an initial 5.00 mg of soluble beta-lactamase were used in the immobilization reaction for each preparation, and average coupling yield was 80.5%. of the enzyme immobilized on the matrix, an average 53.4% remained active. to minimize diffusional effects on immobilized enzyme activity, reaction mixtures were rotated at 250 rpm throughout the study. the shape of the ph activity curve of the i ...19873500195
[effect of components of curing mixtures on the antagonistic properties of enterococci and other bacteria isolated from cured meat].effect of sodium chloride, glucose, sodium glutamate, potassium nitrate, sodium nitrate, sodium nitraite, ascorbic acid, sodium citrate, polyphosphates (hamine) on antagonistic activity of enterococci and various bacteria isolated from cured meat against bacillus cereus, bacillus subtilis, salmonella typhi-murium, salmonella cholerae-suis, staphylococcus aureus, clostridium botulinum type a, clostridium botulinum type b, clostridium botulinum type e, clostridium bifermentans, clostridium perfrin ...19873506173
metastatic bacterial endophthalmitis: a contemporary reappraisal.metastatic bacterial endophthalmitis remains a challenge to the clinician despite the success of antibiotics in reducing its frequency and severity. controversy currently surrounds the management of this condition because of uncertainty about the value of and indications for vitreous surgery. we review 72 cases of metastatic endophthalmitis from the past decade, including five not previously published. the spectrum of causative bacteria changed significantly during this period, with displacement ...19863541265
excretion of oxytetracycline in eggs after medication of laying hens.the kinetics of oxytetracycline elimination into eggs were determined separately for albumen and yolk after oral administration through either drinking water (0.1-0.25 and 0.5 g/l for 5 days) or feed (300 and 600 ppm for 7 days) or after intramuscular injections (3 x 15 mg/kg body weight and 3 x 30 mg/kg body weight), 24 hours apart. residues were assayed by a microbiological agar diffusion method, with bacillus cereus as test-organism. the detection threshold was 0.07 micrograms/g for albumen a ...19873653454
the membrane-anchor of paramecium temperature-specific surface antigens is a glycosylinositol phospholipid.the temperature-specific g surface antigen of paramecium primaurelia strain 156 was biosynthetically labeled by [3h]myristic acid in its membrane-bound form, but not in its soluble form. it could be cleaved by a phosphatidylinositol-specific phospholipase c from trypanosoma brucei or from bacillus cereus which released its soluble form with the unmasking of a particular glycosidic immunodeterminant called the crossreacting determinant. the paramecium enzyme, capable of converting its membrane-bo ...19873663213
in-vitro activity of ru 28965, a new macrolide, compared to that of erythromycin.the activity of a novel macrolide, ru 28965, was compared to that of erythromycin against gram-positive organisms isolated mainly from cancer patients. ru 28965 was active against streptococci including streptococcus pneumoniae and streptococcal groups a, b and g. it was also active against methicillin-susceptible staphylococci, bacillus cereus and listeria monocytogenes. its activity against these organisms was generally equal to that of erythromycin. ru 28965 was moderately active against ente ...19863700283
the major surface protein of leishmania promastigotes is anchored in the membrane by a myristic acid-labeled phospholipid.promastigotes of the protozoan parasite leishmania major were biosynthetically labeled with myristic acid. solubilization and phase separation in the non-ionic detergent triton x-114 shows that the label is not incorporated into soluble hydrophilic proteins, but is incorporated into a few insoluble proteins. the bulk of the incorporated fatty acid is associated with a heterogeneous phosphorylated glycolipid and a few amphiphilic integral membrane proteins. among these, the major surface protein ...19863709520
a novel nontoxic alkyl-phospholipid with selective antitumor activity, plasmanyl-(n-acyl)-ethanolamine (pnae), isolated from degenerating chick embryonal tissues and from an anticancer biopreparation cacpl.a novel alkyl-phospholipid with selective antitumor activity was isolated from an anticancer biopreparation cacpl (crude anticancer phospholipids) and from tissues of degenerating chick embryos. the alkyl-phospholipid was isolated and purified by chromatographic methods using silicic acid column chromatography and thin layer chromatography. the chemical structure of the alkyl-phospholipid was characterized by thin-layer chromatographic analysis of the degradation products after enzymatic digesti ...19863713923
effect of phospholipase c on high-molecular-mass alkaline phosphatase in serum.electrophoresis of some serum samples on polyacrylamide gel, followed by staining for alkaline phosphatase (ec 3.1.3.1), produces a band of activity at the gel origin. this high-mr band consists of liver membrane fragments containing alkaline phosphatase and other enzymes. alkaline phosphatase is closely associated with phosphatidylinositol in liver plasma membranes, and we have found that phospholipase c (ec 3.1.4.3) from bacillus cereus, known to possess some phosphatidylinositol specificity, ...19863731443
interplay between lipids and viral glycoproteins during hemolysis and fusion by influenza virus.since mixtures of lipids alone are known to elicit membrane fusion without participation of fusion proteins, the role of viral lipids in the so-called virus-induced hemolysis and cell fusion has been investigated, using as a model the fowl plague virus (influenza a/fpv/rostock/h7n1). the experiments were planned in a way that allowed quantitative modification of viral lipids without changing envelope glycoproteins. under the conditions employed, cholesterol oxidase of nocardia erythropolis and p ...19863759942
ultrastructural observations on peritoneal exudate cells from the striped bass.an ultrastructural study was conducted of the principal cells recovered from the peritoneal exudate of striped bass (morone saxatilis) injected with killed bacillus cereus. the most frequently observed cells were identified as macrophages, eosinophils, and neutrophils on the basis of comparing their structure with that reported for other fish species and higher vertebrates. all three of the types of cells were phagocytic; however, the numbers of bacteria engulfed were limited. like other studies ...19863765336
ovine pulmonary transit of tetracycline and minocycline.following cannulation of the right external jugular vein and the efferent duct of the right caudal mediastinal lymph node (the caudal end of this node was ligated to cut off the inflow of systemic lymph, i.e., 90%-95% of the efferent lymph was of pulmonary origin), sheep were given either tetracycline or minocycline as single doses of 5 mg/kg body weight infused intravenously over 30 min. venous blood plasma and pulmonary lymph collected contemporaneously before infusion and from 5 min to 24 hr ...19873802746
release of the 120 kda component of the mouse neural cell adhesion molecule n-cam from cell surfaces by phosphatidylinositol-specific phospholipase c.to study the membrane anchoring of the 120 kda component of the neural cell adhesion molecule n-cam, the smallest form lacking a transmembrane domain, cultured mouse neural cells were treated with phosphatidylinositol-specific phospholipase c from staphylococcus aureus. when live cultures of astrocytes and neurons are treated with phosphatidylinositol-specific phospholipase c, n-cam120 is released into the supernatant. under these conditions n-cam140 and n-cam180 are not released. phospholipase ...19863822237
isolation and partial characterization of biologically active fc receptor of chicken red cells.it has previously been demonstrated that chicken red cells have a receptor with the capacity to bind aggregated igg, igm 7 s or antigen-complex igg. this receptor was isolated from nonidet p-40 soluble extracts of chicken red cells by immunoadsorption with either immobilized aggregated igg or monomeric igm (igm 7 s) and further gel filtration through a sephacryl s-300 column. the fc binding material was characterized as a glycoprotein with a molecular weight of 30,000 which retained its fc recep ...19873828381
murine monoclonal anti-dna autoantibodies bind to endogenous bacteria.several bacterial species (including streptococcus faecalis, bacillus cereus, staphylococcus aureus, and escherichia coli) were tested for their ability to react with monoclonal anti-dna antibodies that were derived from mrl-lpr/lpr mice. s. faecalis reacted with 8/15 of such antibodies. the binding was unaffected by dnase, but it was competitively inhibited by dna. f(ab')2 fragments of the monoclonal antibodies reacted with the bacteria, but fc fragments did not. phospholipids extracted from th ...19853874226
processing of bacillus cereus 569/h beta-lactamase i in escherichia coli and bacillus subtilis.the gene for bacillus cereus 569/h beta-lactamase i, penpc, has recently been cloned and sequenced (mézes, p. s. f., yang, y. q., hussain, m., and lampen, j. o. (1983) febs lett. 161, 195-200). a typical prokaryotic signal peptide but with no lipoprotein modification site, as present in the bacillus licheniformis 749/c beta-lactamase, was indicated by the dna sequence for this secretory protein. we have here purified the beta-lactamase i products found in escherichia coli and bacillus subtilis c ...19853918030
polymer-level synthesis of oxopyrimidine deoxynucleotides by bacillus subtilis phage sp10: characterization of modification-defective mutants.bacillus subtilis phage sp10 dna has two oxopyrimidines, thymidine 5'-monophosphate (dtmp) and its hypermodified analog (ydtmp). published data suggest that both are synthesized by postreplicational modification of 5-hydroxymethyldeoxyuridylate (homedump) in nascent dna by the following pathway: homedump----ppomedump----dtmp (85%) or ydtmp (15%); ppomedump is 5-(hydroxymethyl-o-pyrophosphoryl)deoxyuridylate, the pyrophosphoric acid ester of the c5ch2oh function of homedump. this paper describes ...19853918174
antimicrobial activity of bupivacaine and morphine.antimicrobial activity of bupivacaine and morphine against 10 microbial strains was studied with an agar dilution method. the strains tested were escherichia coli (atcc 25922), pseudomonas aeruginosa (atcc 27853), staphylococcus aureus (atcc 25923), and one of each of the clinical isolates of staphylococcus epidermidis (a multiresistant strain), staphylococcus epidermidis (a sensitive strain), streptococcus pneumoniae, streptococcus pyogenes (a), streptococcus faecalis, bacillus cereus, and cand ...19853918482
microbiological aspects of goat's milk. a public health laboratory service survey.in a 12-month survey (june 1982-may 1983) 41 laboratories examined 2493 samples of goat's milk for colony counts and the presence of pathogens. the statutory tests for cow's milk were also applied. surface counts of less than 10(5) organisms per ml of raw milk were given by 79% of samples at 37 degrees c and by 76% at 22 degrees c. there were less than 100 coliforms per ml in 71% of samples, less than 10 escherichia coli per ml in 91%. staphylococcus aureus was not detected in countable numbers ...19853919086
life-threatening complications associated with bacillus cereus pneumonia.bacillus species are identified as pathogens in lung and pleural space infections with increasing frequency. we report a patient who developed life-threatening complications of bacillus cereus pneumonia, including massive hemoptysis, acute respiratory failure, tension pneumothorax, empyema, and bronchopleural fistula. we also review the pertinent literature concerning the associated underlying disorders, complications, and therapy of bacillus species pulmonary infections.19853919626
properties and origin of filamentous appendages on spores of bacillus cereus.some physical, chemical, and immunological properties of filamentous appendages and the exosporium on the spores of bacillus cereus were examined for the purpose of elucidating the origin of filamentous appendages. the main components of both filamentous appendages and the exosporium were protein and their amino acid compositions were similar in point of a high content of glycine, alanine, threonine, valine, and acidic amino acids and a low content of basic and sulphur-containing amino acids. tr ...19853921809
[properties of the phospholipases c from bacillus cereus].three different phospholipases c--the so-called phospholipase c which hydrolyses phosphatidylcholine (pch-plc), sphingomyelinase (sm-plc) and phosphatidylinositol hydrolysing phospholipase c (pi-plc)--were separated from the culture filtrate of bacillus cereus using column chromatography on deae-sephadex a-50. the pi values were estimated to be 5.0 +/- 0.3 for pi-plc and 5.3 +/- 0.2 for sm-plc. the effect of some bivalent cations was studied. metal ions had no effect on the activity of pi-plc, w ...19853921953
[occurrence of enteritis-causing agents in private households--a pilot study].the increasing occurrence of infectious enteritis caused us to perform a prospective, epidemiological pilotstudy in households, in order to receive informations about the practicability of such a project and the bacteriology of food prepared at home as well. 10 families were asked to gather samples of all foodstuffs consumed during the day in separate containers and to keep them frozen at - 20 degrees c until collected by us once a week. thus we received 4.683 samples within a 6-months-period. i ...19853922161
bacillus cereus, diarrhea, and home-dried apples. 19853922272
demonstration of a capsule plasmid in bacillus anthracis.virulent and certain avirulent strains of bacillus anthracis harbor a plasmid, designated pxo2, which is involved in the synthesis of capsules. two classes of rough, noncapsulated (cap-) variants were isolated from the capsule-producing (cap+) pasteur vaccine strains atcc 6602 and atcc 4229. one class was cured of pxo2, and the other class still carried it. reversion to cap+ was demonstrable only in rough variants which had retained pxo2. proof that pxo2 is involved in capsule synthesis came fro ...19853926644
on the mechanism of cereobiogen readjustment to dysbiosis. 19853927307
bacillus cereus panophthalmitis appearing as acute glaucoma in a drug addict. 19853927739
dissemination of bacillus cereus in a maternity unit.during a period of 4 months, 54 of 1059 (5%) specimens received for bacterial culture from neonates in a maternity unit yielded bacillus cereus. of the strains isolated, 48 originated from 259 umbilical cord swabs examined. furthermore, 88% of the isolates belonged to a single, previously undescribed serotype. strains of the same serotype were also found to be widely distributed in the environment throughout the maternity unit. the primary source of the organism, however, could not be establishe ...19853928765
charged detergents enhance the activity of phospholipase c (bacillus cereus) towards micellar short-chain phosphatidylcholine.phospholipase c (phosphatidylcholine cholinephosphohydrolase, ec 3.1.4.3) (bacillus cereus) activity toward diheptanoylphosphatidylcholine is increased 50-100% by low concentrations of both positively and negatively charged detergents. zwitterionic and nonionic detergents have no such activating effect. this charged detergent activation requires an interface, since comparable detergent concentrations have no effect on the hydrolysis rate of monomeric dihexanoylphosphatidylcholine. from nmr and d ...19853929839
the amino acid sequence of the zinc-requiring beta-lactamase ii from the bacterium bacillus cereus 569.the amino acid sequence of the zinc-requiring beta-lactamase ii from bacillus cereus strain 569 has been determined. it consists of a single polypeptide chain of 227 residues. it is the only example so far fully characterized of a class b beta-lactamase, and is structurally and mechanistically distinct from both the widely distributed class a beta-lactamases (such as the escherichia coli rtem enzyme) and from the chromosomally encoded class c enzymes from gram-negative bacteria.19853930290
cloning and sequencing of the metallothioprotein beta-lactamase ii gene of bacillus cereus 569/h in escherichia coli.the structural gene for beta-lactamase ii (ec 3.5.2.6), a metallothioenzyme, from bacillus cereus 569/h (constitutive for high production of the enzyme) was cloned in escherichia coli, and the nucleotide sequence was determined. this is the first class b beta-lactamase whose primary structure has been reported. the amino acid sequence of the exoenzyme form, deduced from the dna, indicates that beta-lactamase ii, like other secreted proteins, is synthesized as a precursor with a 30-amino acid n-t ...19853930467
trypsinlike enzymes from dormant and germinated spores of bacillus cereus t and their possible involvement in germination.trypsin-like enzymes were studied in dormant, activated, and germinated spores of bacillus cereus t. dormant spores contained two heat-labile enzyme activities. one was extractable with 2 m kcl and hydrolyzed azo-albumin. the second, a trypsinlike activity, was not extractable with 2 m kcl and hydrolyzed benzoyl-l-arginine-p-nitroanilide. because of their heat instability, these two enzyme activities are probably not involved in the germination of heat-activated spores. upon germination of heat- ...19853930468
use of microbial beta-lactamase to destroy penicillin added to milk.a simple method is described for destruction of penicillin residues in bulk milk to an undetectable amount (less than .003 u/ml) with commercially available crude beta-lactamase enzyme. milk containing .1 or .5 u/ml penicillin g was treated with .01 to 1.0 mu/ml of beta-lactamase (bacillus cereus) for up to 96 h. the bacillus stearothermophilus var. calidolactis assay was used to quantify penicillin in milk between .003 to 1.0 u/ml. the .5 u/ml of penicillin g was reduced to an undetectable amou ...19853930584
[role of ribosidase in germination of bacillus cereus t spores induced by l-alanine and inosine]. 19853930808
the relation between the diarrheal and other biological activities of bacillus cereus involved in food poisoning outbreaks. 19853930821
aminoglycosides and penicillins can be inactivated by one enzyme, penicillinase. 19853930974
[changes in the structural function of the membrane of m. lysodeikticus as affected by preparations of the bacterial autoregulatory factors d1].introduction of factors d1 isolated from the culture fluids of b. cereus and ps. carboxydoflava into cytoplasmic membranes of m. lysodeikticus resulted in a higher microviscosity of the lipid phase. factor d1 changes the ion permeability of an artificial bilayer membrane as well as reduces the content of free water in the bacterial paste. it is assumed that registered structural changes may result from the interaction of the factor molecules with membrane lipids and be the main reason of changes ...19853931069
inhibition of germinant binding by bacterial spores in acidic environments.commitment to germinate occurred in both clostridium botulinum and bacillus cereus spores during 0.5 min of exposure to 100 mm l-alanine or l-cysteine, measured by the inability of germination inhibitors (d form of amino acid) to inhibit germination. spore germination at ph 4.5 was inhibited because the germinant did not bind to the trigger sites. c. botulinum spores exposed to 100 mm l-alanine or l-cysteine at ph 4.5 remained sensitive to d-amino acid inhibition at ph 7, indicating that no germ ...19853931549
the production and molecular properties of the zinc beta-lactamase of pseudomonas maltophilia iid 1275.the production and purification of a tetrameric zinc beta-lactamase from pseudomonas maltophilia iid 1275 were greatly improved. three charge variants were isolated by chromatofocusing. the subunits each contain two atomic proportions of zinc and (in two of the variants) one residue of cysteine. the thiol group is not required for activity, nor does it appear to bind to the metal. replacement of zinc by cobalt, cadmium or nickel takes place at a measurable rate, and gives enzymes that are less a ...19853931629
single-turnover and steady-state kinetics of hydrolysis of cephalosporins by beta-lactamase i from bacillus cereus.the kinetics of the hydrolysis of two cephalosporins by beta-lactamase i from bacillus cereus 569/h/9 has been studied by single-turnover and steady-state methods. single-turnover kinetics could be measured over the time scale of minutes when cephalosporin c was the substrate. the other substrate, 7-(2',4'-dinitrophenylamino)deacetoxycephalosporanic acid, was hydrolysed even more slowly, and has potential for use in crystallographic studies of beta-lactamases. comparison of single-turnover and s ...19853933490
sensitivity of phospholipase c (bacillus cereus) activity to phosphatidylcholine structural modifications.the structural features of a phosphatidylcholine molecule important for binding to phospholipase c (bacillus cereus) have been examined using kinetic analyses of a series of short-chain phosphatidylcholines and analogues. lipids examined had varying chain lengths, methyl branched chains, phenyl alkanoate chains, and a single fatty acyl chain (lysophosphatidylcholines). a comparison of vmax and km for monomolecularly dispersed dibutyroyl-, dihexanoyl- and diheptanoylphosphatidylcholine indicates ...19853933566
bovine abortion caused by bacillus cereus.bacillus cereus was identified as an infrequent abortigenic agent in cattle. necrotizing placentitis with no or sporadic lesions in fetal tissues was seen. bacillus cereus was isolated in pure culture from fetal tissue and/or placenta. the recent identification of a bovine abortion caused by b cereus, prompted a retrospective survey of the pathology files. eight of 947 bovine abortions were attributed to b cereus. bacillus cereus is often mistaken as a contaminant in bovine abortion because of t ...19853934117
food poisoning and other clinical diseases caused by bacillus cereus. 19853934309
[comparative biochemistry of microbial base-specific ribonucleases]. 19853934711
cryoenzymology of bacillus cereus beta-lactamase ii.the effects of cryosolvents and subzero temperatures on the metalloenzyme beta-lactamase ii from bacillus cereus have been investigated. preliminary experiments led to the selection of suitable systems for the study of beta-lactamase ii catalysis at low temperatures, namely, cobalt(ii) beta-lactamase ii hydrolysis of benzylpenicillin in 60% (v/v) ethylene glycol and zinc beta-lactamase ii hydrolysis of the chromophoric cephalosporin nitrocefin in 60% (v/v) methanol. progress curves for the hydro ...19853935166
studies on the procoagulant activity of human amniotic fluid. ii. the role of factor vii.partially purified amniotic fluid factor (aff) is found to directly activate human factor x, with a km for factor x of 0.37 +/- 0.06 m. added phospholipid has only a slight effect on the activation at low concentrations, and inhibits the reaction at higher concentrations. both dipf and pmsf inhibit aff factor x activation. although added phospholipid is not required for aff-activity, phospholipase c rapidly destroys it, indicating the presence of intrinsic phospholipid. phospholipase c treated a ...19853936221
[electrophoretic method of demonstrating antibiotic residues in meat and eggs].studies were carried out on smither and vanghan's electrophoretic method with the use of high voltage. the method was found to be readily reproducible and to have good sensitivity. it could be successfully used in the demonstration and identification of residual amounts of antibiotics in meat and eggs. it was also established that with the use of agarose of ph 6.0 amino glycosides, beta-lactams, flavomycin, spectinomycin, and monensin showed higher migration capacity. the same value of ph was co ...19853936255
bacillus cereus pleuropulmonary infection in a normal host. 19853936280
multiple modes of inhibition of spore germination and outgrowth by reduced ph and sorbate.germination and outgrowth of three strains of clostridium botulinum in pyeg medium were measured by phase contrast microscopy. reduction in ph from 7 to 5.5 completely inhibited germination of strain 12885a, reduced the extent of germination of strain 62a and had no effect on the extent of germination of strain 53b. at ph 5.5, 225 mg/l of undissociated sorbic acid had no effect on the germination of strain 53b, while at ph 6.5, 225 mg/l of undissociated sorbic acid completely inhibited germinati ...19853936834
[evaluation of the incidence and toxicity of samples of bacillus cereus in various classes of foods sold and consumed in the state of rio de janeiro].one hundred and fourteen strains of bacillus cereus were isolated during presumptive plate-counts from 18 groups of industrialized, non-industrialized, crude or cooked food, belonging to 10 separate classes. specific presumptive counts ranged from 10(2) to 6 x 10(3)/g or ml. among these isolates, 13 strains were derived from 3 outbreaks of food poisoning (involving a minimum of 57 people), as determined by the assayed bacteriological quality of the ingested foods. as an adopted procedure to corr ...19853937011
isolation and enumeration of b. cereus and some other micro-organisms from balady bread. 19853937343
intestinal carriage of bacillus cereus: faecal isolation studies in three population groups.the results of examinations of stools for bacillus cereus among three unrelated groups of individuals are presented. the groups consisted of (1) healthy school-children aged 6-11 years in a rural region of south africa examined during each of the four seasons of the year; (2) 15 healthy volunteers comprising staff of a london microbiology laboratory and their families examined on each of 3 consecutive weeks; (3) 75 unrelated young children, 2 months to 5 years of age, in a second rural region of ...19853937856
investigations on the sporicidal and fungicidal activity of disinfectants.the sporicidal and fungicidal activity of disinfectants was studied in a suspension test. glutaraldehyde 4%, sodium-dichloroisocyanurate-dihydrate (2400 ppm active chlorine) and peracetic acid 0.25% demonstrated after 30 min of exposure at 20 degrees c in the presence of 4% horse serum a clear activity against spores of bacillus cereus. under the same conditions formaldehyde 4% and glutaraldehyde 2% were also found to be sporicidal, but only after a longer time of exposure. spores of bacillus an ...19853938146
nucleotide sequence of the beta-lactamase gene of alkalophilic bacillus sp. strain 170.a gene for the beta-lactamase from alkalophilic bacillus sp. strain 170 was cloned in a functional state on a 1.0 kb dna fragment and its nucleotide sequence was determined. the coding sequence showed an open reading frame of 257 amino acids, which represents the beta-lactamase precursor protein. it is considered that the signal peptide consisted of 30 amino acids including 12 hydrophobic amino acids.19853938474
mechanism of ca2+ and dipicolinic acid requirement for l-alanine induced germination of bacillus cereus bis-59 spores.spores prepared from different sporulating media containing varying amounts of ca and dipicolinic acid (dpa), exhibited differential responses to germination in l-alanine (0.25 m). ca-spores with moderately high ca and dpa contents could be triggered to germination by l-alanine, whereas p-spores with low contents of ca and dpa could not be germinated by l-alanine unless ca2+ or dpa was exogenously added. the initiation of l-alanine induced germination by p-spores in the presence of 45cacl2 was a ...19853938515
[food poisoning caused by bacillus cereus].an outbreak of food poisoning was observed in sivas on march 27, 1985 involving 25 patients who work at the same place. clinical picture was characterized with nausea, abdominal pain and watery diarrhea. b. cereus was isolated from the stool of 4 patients. no other enteropathogens were found.19853938520
[protoplast fusion in bacillus cereus].polyethyleneglycol induced fusion of bacillus cereus protoplasts and its genetic consequences have been investigated. the technique used allows the transfer of a small plasmid pbc16 between bacillus cereus cells. fusion has resulted in isolation of hybrid cells having acquired tcr phenotype (harbouring pbc16) with high frequencies (10(-2)-10(-3)). however, the genetic instability of hybrid cells and segregation effects have influenced dramatically the final results of plasmid transfer. neverthel ...19853939571
[effect of the relative humidity of the air and of the attendant microflora on aflatoxin formation in food concentrates]. 19853939832
methyl branching in short-chain lecithins: are both chains important for effective phospholipase a2 activity?several seven-carbon fatty acyl lecithins with varied acyl chain branching have been synthesized and characterized as potential phospholipase a2 substrates. micellar bis(4,4-dimethylpentanoyl) phosphatidylcholine, bis(5-methylhexanoyl)phosphatidylcholine, bis(3-methylhexanoyl)phosphatidylcholine, and bis(2-methylhexanoyl)phosphatidylcholine are poor substrates for phospholipase a2 (naja naja naja). these branched lecithins also inhibit the hydrolysis of diheptanoylphosphatidylcholine by the enzy ...19853986178
mating system for transfer of plasmids among bacillus anthracis, bacillus cereus, and bacillus thuringiensis.to facilitate the analysis of genetic determinants carried by large resident plasmids of bacillus anthracis, a mating system was developed which promotes plasmid transfer among strains of b. anthracis, b. cereus, and b. thuringiensis. transfer of the selectable tetracycline resistance plasmid pbc16 and other plasmids from b. thuringiensis to b. anthracis and b. cereus recipients occurred during mixed incubation in broth. two plasmids, pxo11 and pxo12, found in b. thuringiensis were responsible f ...19853988702
localization of phosphatidylcholine in outer envelope membrane of spinach chloroplasts.we have examined the effects of phospholipase c from bacillus cereus on the extent of phospholipid hydrolysis in envelope membrane vesicles and in intact chloroplasts. when isolated envelope vesicles were incubated in presence of phospholipase c, phosphatidylcholine and phosphatidylglycerol, but not phosphatidylinositol, were totally converted into diacylglycerol if they were available to the enzyme (i.e., when the vesicles were sonicated in presence of phospholipase c). these experiments demons ...19853988805
beta-casomorphin immunoreactive materials in cows' milk incubated with various bacterial species.to obtain information about the possible release of beta-casomorphins from beta-casein under in vitro conditions, cows' milk was incubated with 13 strains of gram-negative or gram-positive bacterial species isolated from bovine milk. after incubation periods of 1-24 d, milk samples were assayed for beta-casomorphin-4, -5, -6 or -7 immunoreactive materials. in general, no beta-casomorphin immunoreactive material was found in samples incubated with non-caseolytic strains, e.g. pseudomonas putida o ...19853989066
electrophoretic characterization of hepatic alkaline phosphatase released by phosphatidylinositol-specific phospholipase c. a comparison with liver membrane and serum-soluble forms.alkaline phosphatase was solubilized from plasma membrane of rat liver with butanol-ol, bile acids or sodium deoxycholate, and electrophoretically compared with a soluble form in serum which was derived from the liver. the three enzyme preparations from the plasma membrane migrated at the same position on polyacrylamide-gel electrophoresis in the presence of either triton x-100 or sodium dodecyl sulphate. the mobility of them, however, was distinctly different from that of the serum-soluble form ...19853994680
studies on phospholipase a inhibitor in blood plasma. ii. interaction of phospholipase a inhibitor with phospholipase a and its specificity.non-competitive inhibition of snake venom phospholipase a2 which has been exhibited by bovine plasma phospholipase a inhibitor, a kind of lipoprotein, was not observed unless the inhibitor was preincubated with the enzyme. the inhibition seemed to be due to the formation of the enzyme-inhibitor complex, which was identified by immunoelectrophoresis. the enzyme-inhibitor interaction was observed maximally on incubation at physiological ph, but not below ph 5. the inhibitor was inactivated by tryp ...19854044547
[determination of the killing rate of bacillus spores by peracetic acid].the sporocidal properties of peracetic acid (paa) at defined concentrations were characterized by determination of decrease in paa after addition of d-glucose, human albumin and suspensions of spores; concentration of paa, which inactivates 10(6)-10(7) spores of bacillus cereus, b. subtilis, b. megaterium and b. licheniformis within 10-30 min; inactivation constant k, decimal reduction time d and the sporocidal index (mg paa x min x ml-1) at that concentration. in contrast to albumin, the spore ...19854050179
antibiotic identification by high voltage electrophoresis bioautography.the high voltage electrophoresis bioautography method is applicable to meat, milk, and animal feeds. meat is freeze-dried, powdered, and extracted with acetonitrile-water (9 + 1), and the extract is concentrated by evaporation at room temperature. milk is examined directly or following acetonitrile-water extraction. feed is extracted with acetonitrile-water. samples or extracts are applied to preliminary assay plates of antibiotic medium no. 1 at ph 6 and 8, seeded with micrococcus luteus (atcc ...19854055617
[antibacterial properties of some spice plants before and after heat treatment].this study was carried out to understand the antibacterial properties of some spice plants before and after heat treatment in boiling water. the samples included the core and the outer layers of onion, the white and the green parts of green onion, garlic bulb, ginger, ginger root, sweet pepper, chili pepper, brown pepper, and mustard. the test microorganisms included escherichia coli, salmonella typhimurium, vibrio parahaemolyticus, pseudomonas aeruginosa, proteus vulgaris, staphylococcus aureus ...19854064797
a preliminary survey of aerobic bacteria in breast milk of mothers from the low-income group in nigeria.the aerobic bacteria colonizing breast milk of the low-income group in nigeria were quantified to assess its suitability for use in milk banks. in parallel, the nutritional and health status of donating mothers and their infants were assessed by physicians. the aerobic bacteria contained in the specimens included streptococcus salivarius, bacillus cereus, klebsiella pneumoniae, enterobacter aerogenes, streptococcus pneumoniae, streptococcus pyogenes, pseudomonas aeruginosa and staphylococcus epi ...19854071647
rapid detection of small numbers of airborne bacteria by a membrane filter fluorescent-antibody technique.bacteria from stirred settling aerosols were recovered and identified by a rapid fluorescent-antibody technique previously described by danielsson. calibrated air samples from serratia marcescens aerosols were drawn through a millipore aerosol filter holder fitted out with a nonfluorescent membrane filter (type hab 047). with the aid of a high-power incident-light ultraviolet microscope, the individual bacterial cells, trapped on the filter, could be inspected after staining with appropriate con ...19704100090
regulation of extracellular protease production in bacillus cereus t: characterization of mutants producing altered amounts of protease.twenty-nine mutants of bacillus cereus t were selected on casein agar for their inability to produce large amounts of extracellular protease. they all formed spores, and 27 were also auxotrophs for purines or pyrimidines. upon reversion to prototrophy, a large fraction regained the capacity to produce protease. conversely, reversion to normal protease production resulted in loss of the purine or pyrimidine requirement in a large fraction of the revertants. one spontaneous low-protease-producing ...19714104235
paracrystalline sheets reaggregated from solubilized exosporium of bacillus cereus.fragments of exosporium, isolated from dormant spores of bacillus cereus, were disintegrated by treatment with sodium dodecyl sulfate (sds) or with phenol and acetic acid. after centrifugation of each preparation, proteins in the supernatant fractions were resolved by disc gel electrophoresis into either two or eight bands, respectively. the sds-solubilized fraction contained spheroidal particles 11 to 44 nm in diameter. when centrifuged until clear, this fraction after dialysis still gave rise ...19714105032
cell surface associated gamma globulins in lymphocytes. ii. the pluripotentiality of mouse spleen lymphocytes. 19714107653
thickened cell walls of bacillus cereus grown in the presence of chloramphenicol: their fate during cell growth. 19714110439
penicillin and cell wall synthesis: a study of bacillus cereus by electron microscopy.the changes in wall structure of a penicillinase micro-constitutive strain of bacillus cereus (569/h/24), on exposure to penicillin, and after its removal by addition of penicillinase, have suggested the following model for the growth of the walls of these cylindrical cells. longitudinal extension is by addition of material to a large and continuously increasing number of growing points uniformly distributed over the cylindrical surface. addition is only in the longitudinal direction so that the ...19724110923
enzymatic lysis in vitro of hyalin deposits in human kidney. 19724111563
immunosuppressive properties of a ribonuclease-containing fraction from bacterial cultures. 19724115966
reverse immune cytoadherence. a technique for the detection of surface-associated -globulins on lymphoid cells. 19714116133
ultrastructural alterations induced by moist heat in bacillus cereus.the ultrastructural alterations induced in vegetative, exponentially growing bacillus cereus cells suspended in broth, by heating at 65 c for 2 and 15 min and 100 c for 5 min, were studied by electron microscopy of thin sections. the following alterations were observed: (i) change in the triple-layered profile of the membranes from the normal asymmetric to a symmetric geometry, appearance of fractures in the membranes, occurrence of prominent myelin-like systems of concentric membranes; (ii) dis ...19724116891
influence of cell-wall thickness on cell division: electron microscopic study with bacillus cereus. 19734121312
ultrastructural study of poly- -hydroxybutyrate granules from bacillus cereus.the freeze-etching technique was used to examine the effects of fracturing and etching on the appearance of poly-beta-hydroxybutyrate granules from bacillus cereus. these granules were examined in extracts isolated by hypochlorite or by sonic treatment, and also in fixed and unfixed intact cells; in the latter case they were compared with granules in thin sections of intact cells. after freeze-fracturing, the diameter of the granules in intact cells was between 240 and 720 nm. the granules consi ...19734123236
electron transport system associated with membranes of bacillus cereus during vegetative growth and sporulation.membranes isolated from bacillus cereus atcc 4342 during vegetative growth and during sporulation contained cytochromes b, c and a + a(3) as well as flavoprotein as determined from reduced-minus-oxidized difference spectra. although there appeared to be no qualitative change in the cytochromes, there was a significant increase in the amount of cytochromes associated with membranes isolated from sporulating cells. succinate and nicotinamide adenine dinucleotide (reduced form) (nadh) reduced the s ...19734125246
production of bacteriophage by temperature-sensitive sporulation mutants of bacillus cereus t.five temperature-sensitive sporulation mutants of bacillus cereus t have been isolated. these mutants are blocked at stage 0 of sporulation at the restrictive temperature (37 c) but are able to sporulate at nearly normal frequencies at the permissive temperature (26 c). a bacteriophage that forms a stable lysogen in the parent strain is induced at increased frequencies in the mutants. this induction is accompanied, in some of the mutants, by a reduction in immunity to the phage. revertants, sele ...19734126824
a dissection of the inhibitory effects of 5-fluorouracil in bacillus cereus. 19734127017
letter: bacillus cereus septicaemia. 19744132505
structure of two phages of bacillus thuringiensis and b. cereus. 19744132606
food-poisoning episodes associated with bacillus cereus in fried rice. 19744133716
identification of small amounts of antibiotics by electrophoresis and bio-autography. 19734134372
cold lability of bacillus cereus bacteriophage cp-51.phage cp-51 was rapidly inactivated when stored at the usual refrigerator temperatures (2 to 4 c) and even more rapidly when exposed to 0 c. the loss in viability resulting from exposure to cold appeared to correlate with the increase in number of phage particles having contracted tails. high concentrations (0.01 m) of mg(2+), ca(2+), or mn(2+) stabilized the phage considerably, but even in the presence of these divalent cations, it was much less stable at 0 c than at 15 c.19744138063
[actinomycin d-o]. 19744142853
[autolysis of bacterial cell surface and its physiology (biosynthesis)]. 19724143920
polysaccharides of the genus bacillus cross-reactive with the capsular polysaccharides of diplococcus pneumoniae type 3, haemophilus influenzae type b, and neisseria meningitidis group a.we studied 174 strains of the genus bacillus for cross-reacting antigens to the capsular polysaccharides of groups a and c meningococcus, types i and iii pneumococcus, and haemophilus influenzae type b. cross-reactions were detected by immunodiffusion in agarose gel by using type-specific antisera and confirmed by absorption and inhibition experiments. of 20 bacillus pumilis strains, six had an antigen cross-reacting with group a meningococcal polysaccharide. other cross-reactions included one s ...19734150383
structural studies relating to the distribution of molecular components in erythrocyte membranes. 19744152595
biosynthesis of branched long-chain fatty acids by species of bacillus: relative activity of three alpha-keto acid substrates and factors affecting chain length. 19744155346
[on the use of actinomycin and proflavine to study ribonucleic acid renewal]. 19644157724
bacillus cereus bacteraemia. a complication of intermittent haemodialysis. 19674163313
location of spore and vegatative antigens of bacillus cereus by means of ferritin-labelled antibodies. 19664163998
lysis of escherichia coli by ethylenediaminetetraacetate and phospholipases as measured by beta-galactosidase activity.a permeaseless mutant of escherichia coli, which produces beta-galactosidase constitutively, was treated briefly with ethylenediaminetetraacetate and then with the phospholipases of bacillus cereus. cell lysis occurred, as indicated by an increase in beta-galactosidase activity and a decrease in absorbancy of the cell suspension. the susceptibility of the cells to attack by ethylenediaminetetraacetate and the phospholipases was markedly affected by the age of the cells when harvested. the result ...19674168313
evolution of developmental stages in cultures of bacillus cereus. 19684173035
the binding of actinomycin d and f to bacterial dna. 19684177549
immunological response in cows to mycoplasma bovigenitalium, strain 1836. 19684179665
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