| immunogen-dependent quantitative and qualitative differences in phagocytic responses of the circulating hemocytes of the lobster homarus americanus. | phagocytic responses in circulating hemocytes of the lobster homarus americanus were measured before and after treatment of lobsters with 2 different immunogens: (1) lipolysaccharide (lps) or endotoxin from a non-pathogenic pseudomonas perolens, and (2) a vancomycin/live gram-positive pathogen (aerococcus viridans [var.] homari) combination, essentially attenuated cells, shown previously to induce a high degree of resistance to this pathogen. the responses elicited by each of the immunogens were ... | 2006 | 16724563 |
| infective endocarditis and spondylodiscitis in a patient due to aerococcus urinae: first report. | | 2007 | 16766062 |
| microbial colonization of tourniquets used in orthopedic surgery. | this study analyzed tourniquets used for orthopedic surgery in our hospital to determine the frequency and type of microbial contamination. group a tourniquets were from our main operating room, group b tourniquets were from our ambulatory surgicenter, group c tourniquets were unused, prepackaged, sterile tourniquets from our main operating room, and group d tourniquets were sterilely packed tourniquets from our ambulatory surgicenter. tourniquets from groups a, b, c, and d had 100%, 40%, 0%, an ... | 2006 | 16924865 |
| the crystal structure of l-lactate oxidase from aerococcus viridans at 2.1a resolution reveals the mechanism of strict substrate recognition. | l-lactate oxidase (lox) from aerococcus viridans is a member of the alpha-hydroxyacid-oxidase flavoenzyme family. we have determined the three-dimensional structure of lox and revealed the mechanism of substrate recognition. the lox monomer structure has a typical alpha(8)/beta(8) motif commonly found in other flavin family proteins. a related enzyme, glycolate oxidase, catalyzes the oxidation of glycolate rather than lactate. comparison of the two enzyme structures highlights the importance of ... | 2006 | 17007814 |
| improving the thermal stability of lactate oxidase by directed evolution. | lactate oxidase is used in biosensors to measure the concentration of lactate in the blood and other body fluids. increasing the thermostability of lactate oxidase can significantly prolong the lifetime of these biosensors. we have previously obtained a variant of lactate oxidase from aerococcus viridans with two mutations (e160g/v198i) that is significantly more thermostable than the wild-type enzyme. here we have attempted to further improve the thermostability of e160g/v198i lactate oxidase u ... | 2007 | 17131051 |
| microbial community structure and dynamics of dark fire-cured tobacco fermentation. | the italian toscano cigar production includes a fermentation step that starts when dark fire-cured tobacco leaves are moistened and mixed with ca. 20% prefermented tobacco to form a 500-kg bulk. the dynamics of the process, lasting ca. 18 days, has never been investigated in detail, and limited information is available on microbiota involved. here we show that toscano fermentation is invariably associated with the following: (i) an increase in temperature, ph, and total microbial population; (ii ... | 2007 | 17142368 |
| the 2.1 a structure of aerococcus viridans l-lactate oxidase (lox). | the crystal structure of l-lactate oxidase (lox) from aerococcus viridans has been determined at 2.1 a resolution. lox catalyzes the flavin mononucleotide (fmn) dependent oxidation of lactate to pyruvate and hydrogen peroxide. lox belongs to the alpha-hydroxy-acid oxidase flavoenzyme family; members of which bind similar substrates and to some extent have conserved catalytic properties and structural motifs. lox crystallized as two tightly packed tetramers in the asymmetric unit, each having fou ... | 2006 | 17142893 |
| serological cross-reactivity of environmental isolates of enterobacter, escherichia, stenotrophomonas, and aerococcus with shigella spp.-specific antisera. | using protocols designed for the isolation of shigella from environmental freshwater samples from different regions of bangladesh, 11 bacterial strains giving rise to shigella-like colonies on selective agar plates and showing serological cross-reaction with shigella-specific antisera were isolated. phylogenetic analyses revealed that three of the isolates were most closely related to escherichia coli, four to enterobacter sp., two to stenotrophomonas, and two isolates belonged to the gram-posit ... | 2007 | 17171463 |
| biodiversity of bacterial ecosystems in traditional egyptian domiati cheese. | bacterial biodiversity occurring in traditional egyptian soft domiati cheese was studied by pcr-temporal temperature gel electrophoresis (ttge) and pcr-denaturing gradient gel electrophoresis (dgge). bands were identified using a reference species database (j.-c. ogier et al., appl. environ. microbiol. 70:5628-5643, 2004); de novo bands having nonidentified migration patterns were identified by dna sequencing. results reveal a novel bacterial profile and extensive bacterial biodiversity in domia ... | 2007 | 17189434 |
| enterococcus and lactobacillus contamination of raw milk in a farm dairy environment. | enterococci and lactobacilli are ubiquitously found in the intestinal microflora of humans and animals. the aim of the present study was to determine the importance of bovine faeces as a source of these organisms in raw milk. one hundred and fifty six putative enterococci and 362 lactobacilli were isolated from bovine faeces (n=26), cows' teats, raw milk, the milking machine and the milking environment on one farm. the clonal relationships of each group were investigated using pulsed-field gel e ... | 2007 | 17189657 |
| diversity of lactic acid bacteria associated with fish and the fish farm environment, established by amplified rrna gene restriction analysis. | lactic acid bacteria have become a major source of concern for aquaculture in recent decades. in addition to true pathogenic species of worldwide significance, such as streptococcus iniae and lactococcus garvieae, several species have been reported to produce occasional fish mortalities in limited geographic areas, and many unidentifiable or ill-defined isolates are regularly isolated from fish or fish products. to clarify the nature and prevalence of different fish-associated bacteria belonging ... | 2007 | 17337536 |
| quantitative analysis of the intestinal bacterial community in one- to three-week-old commercially reared broiler chickens fed conventional or antibiotic-free vegetable-based diets. | to explore the effect of drug-free poultry production on the intestinal microflora of broiler chickens, the bacterial community of this environment was quantitatively profiled in both conventionally reared birds and birds reared without antibiotic growth promotants (agps) on a vegetable-based diet. | 2007 | 17381758 |
| culture-negative infective endocarditis caused by aerococcus urinae. | aerococcus urinae is a rarely reported pathogen that often causes mild urinary tract infection (uti), although serious complications such as endocarditis and septicemia have also been described. the organism may easily be missed or misidentified when using commercial detection systems. a. urinae is resistant to sulfonamides and, therefore, a typical treatment for uti may be inappropriate. to date, 14 cases of a. urinae infective endocarditis (ie) have been reported, most of which were elderly ma ... | 2007 | 17484472 |
| crystallographic study on the interaction of l-lactate oxidase with pyruvate at 1.9 angstrom resolution. | l-lactate oxidase (lox) from aerococcus viridans catalyzes the oxidation of l-lactate to pyruvate by the molecular oxygen and belongs to a large family of 2-hydroxy acid-dependent flavoenzymes. to investigate the interaction of lox with pyruvate in structural details and understand the chemical mechanism of flavin-dependent l-lactate dehydrogenation, the lox-pyruvate complex was crystallized and the crystal structure of the complex has been solved at a resolution of 1.90 angstrom. one pyruvate m ... | 2007 | 17517371 |
| aerococcus suis sp. nov., isolated from clinical specimens from swine. | biochemical and molecular genetic studies were performed for five isolates of unknown gram-positive, catalase-negative, cocci-shaped micro-organisms obtained from clinical samples from pigs. the micro-organisms were tentatively identified as aerococcus species on the basis of the results from cellular morphological and biochemical tests. 16s rrna gene sequencing studies confirmed the provisional identification of the isolates as members of the genus aerococcus, but the micro-organism did not cor ... | 2007 | 17551045 |
| [screening and characterization of marine bacteria with antibacterial and cytotoxic activities, and existence of pks i and nrps genes in bioactive strains]. | antibacterial and cytotoxic activities were screened for marine bacteria which have been isolated from organism, sediments and seawater in china coastal area. the results showed that 42 isolates had antimicrobial activity and 12 isolates had cytotoxicity. molecular phylogenetic analysis of marine bacteria with cytotoxicity based on 16s rrna sequences indicated that they were belong to the genera aerococcus, agrobacterium, alteromona, bacillus, exiguobacterium, paracoccus, pseudoalteromons, rhein ... | 2007 | 17552225 |
| characterization of aerococcus viridans isolates from swine clinical specimens. | we present here the biochemical and genetic characterization and antimicrobial susceptibility of 58 isolates of aerococcus viridans isolated in pure culture from different clinical specimens of normally sterile body sites of pigs. a. viridans isolates were commonly susceptible to beta-lactam antimicrobials and exhibited a great genetic heterogeneity as determined by pulsed-field gel electrophoresis typing. the results indicate that a. viridans might be included in the list of possible etiologica ... | 2007 | 17596355 |
| a taxonomic survey of lactic acid bacteria isolated from wheat (triticum durum) kernels and non-conventional flours. | in order to explore the correspondence between raw material- and mature sourdough-lactic acid bacterial (lab) communities, 59 italian wheat (triticum durum) grain samples, one bran and six non-conventional flour samples were analyzed through a culture-dependent approach. the highest cell count by an agar medium specific for lab was 2.16 log cfu/g. from about 2300 presumptive lab (gram-positive and catalase-negative) colonies collected, a total of 356 isolates were subjected to identification by ... | 2007 | 17683888 |
| molecular phylogenetic diversity of bacteria and its spatial distribution in composts. | to investigate microbial diversity of swine manure composts in the initial stage and the spatial distribution due to gradient effect. | 2007 | 17897238 |
| contamination of milk by enterococci and coliforms from bovine faeces. | to determine the contribution of enterococci and coliforms from bovine faeces and teats to contamination of raw milk. methods: putative enterococci (n = 301) and coliforms (n = 365) were isolated from bovine faeces (n = 20), cows' teats (n = 20), the raw milk (n = 1) and the milking environment (n = 4) on one farm. the clonal relationships of each bacterial group were investigated using pulsed-field gel electrophoresis of genomic macrorestriction fragments. representatives of the different clust ... | 2007 | 17953550 |
| case study: aerococcus urinae as pathogen in peritoneal dialysis peritonitis--a first report. | | 2007 | 17984441 |
| the structures of pyruvate oxidase from aerococcus viridans with cofactors and with a reaction intermediate reveal the flexibility of the active-site tunnel for catalysis. | the crystal structures of pyruvate oxidase from aerococcus viridans (avpox) complexed with flavin adenine dinucleotide (fad), with fad and thiamine diphosphate (thdp) and with fad and the 2-acetyl-thdp intermediate (acthdp) have been determined at 1.6, 1.8 and 1.9 a resolution, respectively. each subunit of the homotetrameric avpox enzyme consists of three domains, as observed in other thdp-dependent enzymes. fad is bound within one subunit in the elongated conformation and with the flavin moiet ... | 2007 | 18007037 |
| spondylodiscitis due to aerococcus viridans. | aerococcus viridans is a microaerophilic, gram-positive, catalase-negative coccus, found singly or in tetrads. to date, no case of spondylodiscitis due to this organism has been reported. we report what we believe to be the first case of spondylodiscitis caused by a. viridans, in a patient with decompensated liver failure, and discuss the possible pathogenesis of this rather uncommon pathogen in this case. | 2008 | 18349378 |
| x-ray structures of aerococcus viridans lactate oxidase and its complex with d-lactate at ph 4.5 show an alpha-hydroxyacid oxidation mechanism. | l-lactate oxidase (lox) belongs to a family of flavin mononucleotide (fmn)-dependent alpha-hydroxy acid-oxidizing enzymes. previously, the crystal structure of lox (ph 8.0) from aerococcus viridans was solved, revealing that the active site residues are located around the fmn. here, we solved the crystal structures of the same enzyme at ph 4.5 and its complex with d-lactate at ph 4.5, in an attempt to analyze the intermediate steps. in the complex structure, the d-lactate resides in the substrat ... | 2008 | 18367206 |
| three cases of serious infection caused by aerococcus urinae: a patient with spontaneous bacterial peritonitis and two patients with bacteremia. | | 2008 | 18463786 |
| isolation and characterisation of two antimicrobial peptides from haemocytes of the american lobster homarus americanus. | two antimicrobial peptides from haemocytes of the american lobster, homarus americanus h. milne edwards 1837, were isolated and partially characterised - the first such description for this species. cap-2, an approximately 12kda peptide, contained amino acid sequences corresponding to the predicted sequence for hoa-crustin. crustins are whey acidic protein (wap) domain - containing peptides isolated from crustacean haemocytes. cap-2 did not have any activity towards the gram positive coccus aero ... | 2008 | 18499476 |
| a quantitative approach to the effectiveness of ozone against microbiota organisms colonizing toothbrushes. | toothbrushes are rapidly contaminated with different microorganisms, which colonize the oral cavity and interdental spaces. this can represent a possible cause of infection or reinfection. in this study, the ozone experimental effect upon toothbrushes microflora was estimated microbiologically before and after saturation with ozone gas. | 2008 | 18502558 |
| acute pyelonephritis caused by aerococcus urinae in a 12-year-old boy. | aerococcus urinae are gram-positive cocci that cause urinary tract infections in adults with underlying genitourinary (gu) tract disease. we report a case of pyelonephritis caused by a. urinae in a 12-year-old boy with a history of pyeloplasty and gu reflux. | 2008 | 18574434 |
| fatal infective endocarditis due to aerococcus urinae--case report and review of literature. | aerococcus-related infective endocarditis is a rare form of endocarditis with a high mortality. most cases have associated genitourinary system pathology. aerococcus urinae is a gram-positive coccus that is arranged in clusters resembling staphylococcus, but its growth characteristics and colonial morphology are closer to that of an alpha-hemolytic streptococcus. this organism may initially be dismissed as a contaminant in clinical cultures from nonsterile sites. we report a representative case ... | 2008 | 18692408 |
| aerococcus urinae in bovine reproductive disease. | | 2008 | 18931362 |
| an automatic flow-injection analysis system for determining phosphate ion in river water using pyruvate oxidase g (from aerococcus viridans). | an automated flow-injection system combining a pyruvate oxidase reaction and chemiluminescence for the detection of phosphate ion in river water has been developed. in this research, we used pyruvate oxidase g (pyroxg), from aerococcus viridans, immobilizing it on n-hydroxysuccinicacidimido beads without a cross-linker. in this sensor system, which was constructed as a trial system of desktop type, the temperature was precisely controlled. after the sensor system was optimized, a calibration cur ... | 1999 | 18967770 |
| aerococcus urinae in ovine reproductive disease. | | 2008 | 19011254 |
| bacterial taxa associated with the hematophagous mite dermanyssus gallinae detected by 16s rrna pcr amplification and ttge fingerprinting. | dermanyssus gallinae (arthropoda, mesostigmata) is suspected to be involved in the transmission of a wide variety of pathogens, but nothing is known about its associated non-pathogenic bacterial community. to address this question, we examined the composition of bacterial communities in d. gallinae collected from standard poultry farms in brittany, france. genetic fingerprints of bacterial communities were generated by temporal temperature gradient gel electrophoresis (ttge) separation of indivi ... | 2009 | 19027065 |
| prevalence of sulfonamide resistance genes in bacterial isolates from manured agricultural soils and pig slurry in the united kingdom. | the prevalences of three sulfonamide resistance genes, sul1, sul2, and sul3 and sulfachloropyridazine (scp) resistance were determined in bacteria isolated from manured agricultural clay soils and slurry samples in the united kingdom over a 2-year period. slurry from tylosin-fed pigs amended with scp and oxytetracycline was used for manuring. isolates positive for sul genes were further screened for the presence of class 1 and 2 integrons. phenotypic resistance to scp was significantly higher in ... | 2009 | 19064898 |
| six cases of aerococcus sanguinicola infection: clinical relevance and bacterial identification. | aerococcus sanguinicola is a gram-positive coccus first described in 2001. infections in humans are rare but the use of 16s rrna gene sequencing and improved phenotypic methods has facilitated the identification of a. sanguinicola. we report here 6 cases of a. sanguinicola bacteraemia, 2 of which were associated with infective endocarditis. most patients were elderly (median age 70 y) and had underlying neurological disorders including dementia, cerebral degeneration, and myelomeningocele. the p ... | 2008 | 19086249 |
| [aerococcus urinae endocarditis: first case report in belgium and review of the literature]. | aerococcus urinae is a rarely reported pathogen, possibly due to difficulties in the identification of the organism. only sixteen cases of a. urinae endocarditis have yet been described. generally, patients were males with predisposing conditions and the outcomes were bad. we describe the first belgian case of endocarditis due to a. urinae. which occurred to a 79-year-old female patient. the diagnostic of a. urinae endocarditis was based on the cardiac ultrasonography and the isolation of the ba ... | 2008 | 19202713 |
| [protease activity of microflora in the oral cavity of patients with periodontitis]. | microbial spectrum and non-specific as well as specific iga1 protease activity of isolated microorganisms were investigated in gingival liquid of patients with periodontitis. microorganisms from the gingival liqud of these patients belonged to conditional-pathogenic obligate and facultatively anaerobic bacteria. 24 strains of microorganisms have been identified. nonspecific proteolytic activity was found in the following microorganisms: actinomyces israelii, actinomyces naeslundii, aerococcus vi ... | 2008 | 19205430 |
| association of streptococcus pluranimalium with valvular endocarditis and septicaemia in adult broiler parents. | the genus streptococcus consists of more than 60 species, but only streptococcus equi subspecies zooepidemicus, streptococcus gallolyticus ssp. gallolyticus, streptococcus gallinaceus, streptococcus dysgalactiae, streptococcus mutans and streptococcus suis have been isolated from poultry. during investigations of the aetiology of increased mortality in broiler parent stock at the end of production, pure cultures of streptococcal-like organisms that could not be classified among these six species ... | 2009 | 19322715 |
| extracorporeal support in a patient with cardiogenic shock due to aerococcus urinae endocarditis. | aerococcus urinae is a newly identified gram-positive coccus that causes serious infections. to date, only 15 cases of a. urinae infective endocarditis have been reported, but with a very high mortality. the case is reported of a patient with a. urinae double valve endocarditis. following aortic and mitral valve replacement, the patient suffered from refractory cardiogenic shock; extracorporeal membrane oxygenation was used successfully as a rescue mechanical support. | 2009 | 19852146 |
| [bacterial diversity recovered from qiyi glacier and runoff, qilian mts]. | abundance and species diversity of bacteria were investigated respectively by epifluorescence microscope, the culture method and the analysis of 16s rdna genes, with snow and runoff samples from qiyi glacier in the qilian mts. according to the results, the total microbial cells and bacterial cfu range from 10(3) to 10(5) cells x ml(-1) and 0-600 cfu x ml(-1), respectively. the 16s rdna gene of 22 bacterial isolates recovered from snow and runoff samples belong to following groups: bacteroidetes, ... | 2009 | 19927833 |
| [microbal reduction of chlorine oxyanions using plant raw material]. | a possibility of chlorates reduction at the joint growing of chlorate-reducing and cellulose-degrading bacteria on the medium with paper as a carbon source was proved. the growth rate of associative culture on the medium with paper was by 18-25% lower than that on mpb. enrichment culture of cellulose-degrading bacteria can reduce up to 10% of added chlorates. ethanol is the cellulose hydrolysis product which is primarily consumed under chlorate reduction; then follows the consumption of acetic a ... | 2009 | 19938597 |
| expression of antimicrobial peptides from hyas araneus haemocytes following bacterial challenge in vitro. | circulating haemocytes play major roles in the host defense reactions of decapods, including the synthesis and release of antimicrobial peptides (amps). unlike the amps from insects, those in decapods are constitutively expressed. this study aims to establish primary cell cultures of the three main haemocyte types in hyas araneus haemocytes, and to measure the in vitro expression of amp genes in the cells following microbial challenge. the haemocyte populations were separated on percoll gradient ... | 2010 | 20083137 |
| molecular analysis of bacterial population structure and dynamics during cold storage of untreated and treated milk. | spoilage bacteria in milk are controlled by treatments such as thermization, microfiltration and addition of carbon dioxide. however, little information is known about the changes in microbial communities during subsequent cold storage of treated milk. culture-dependent methods and a direct molecular approach combining 16s rrna gene clone libraries and quantitative pcr (q-pcr) were applied to obtain a better overview of the structure and the dynamics of milk microbiota. raw milk samples were tre ... | 2010 | 20137820 |
| [chlorate reduction by immobilised bacteria in continuous conditions]. | chlorate reduction by the strain aerococcus dechloraticans tgs-463 immobilized on the corncob under flow conditions has been studied. it has been established that under growth in the medium with chlorate the increase of the dilution rate (d) results in the lower efficacy of the process, i.e., a higher concentration of residual chlorates in the medium and a lower rate of chlorate reduction. the optimal d for chlorate reduction ranges from 1.12 to 1.5 hour(-1). | 2010 | 20364714 |
| a case of endocarditis due to aerococcus urinae. | | 2010 | 20369517 |
| [post-operative bacteremia caused by multidrug-resistant aerococcus viridans in a patient with gall bladder cancer]. | aerococcus viridans is a gram-positive, catalase and oxidase negative, microaerophylic and non-motile coccus which is rarely associated with human infections such as endocarditis, meningitis, artritis and bacteremia. we report a case of bacteremia due to a. viridans in a 61-years-old man with malignant gall bladder neoplasm. the patient underwent a surgical operation and on the 5th day of operation he had severe abdominal pain, vomiting, high fever and discharge from operation site. he was trans ... | 2010 | 20455408 |
| [effect of sewage components of match production on chlorate-reducing activity of aerococcus dechloraticans tgs-463]. | a mathematical model has been offered which describes adequately the process of chlorates reduction by aerococcus dechloraticans tgs-463 culture, the component of sewage from production of ignition mixtures being available in the medium. it is shown that chromates, trivalent iron and zinc take the most negative effect on the rate of chlorates utilization (by the degree of decrease). phosphates have no effect on chlorate-reduction. | 2009 | 20458930 |
| aerococcus urinae and aerococcus sanguinicola, two frequently misidentified uropathogens. | species belonging to the aerococcus genus are isolated from the urine and blood of elderly patients suffering from urinary tract infections (utis). however, the clinical significance, phenotypic features and antimicrobial susceptibilities of these underestimated and/or misidentified species remain unclear. from march 2006 to november 2008, among 350 non-enterococcal streptococcaceae species isolated from urinary specimens, 30 (8.6%) aerococcus spp. strains were recovered. all strains were charac ... | 2010 | 20482457 |
| investigation of mineral water springs of miercurea ciuc (csíkszereda) region (romania) with cultivation-dependent microbiological methods. | water samples of ten mineral water springs at miercurea ciuc (csíkszereda) region (romania) were examined during 2005-2006 using cultivation-dependent microbiological methods. the results of standard hygienic bacteriological tests showed that the hargita spring had perfect and five other springs had microbiologically acceptable water quality (zsögöd-, nagy-borvíz-, taploca-, szentegyháza- and lobogó springs). the water of borsáros spring was exceptionable (high germ count, presence of enterococc ... | 2010 | 20587384 |
| rapid paper disk test for identification of helicobacter pylori in mixed cultures of gerbil gastric homogenates. | a method denominated rapid paper disk test (rpdt) was developed to identify h. pylori colonies in complex cultures obtained from gerbil gastric homogenates. identification is based on a characteristic reaction pattern (rp) for h. pylori colonies given by the combination of the urease-oxidase activities on a paper disk. compared to the rps obtained from gerbil's intestinal tract isolated bacteria, h. pylori rp is completely distinguishable, even from those of bacteria that share one or both activ ... | 2010 | 20624429 |
| aerococcus urinae: severe and fatal bloodstream infections and endocarditis. | aerococcus urinae is a pathogen that rarely causes severe or fatal infections. we describe four cases of severe a. urinae bloodstream infections. all patients had underlying urologic conditions. urine cultures, however, were negative. | 2010 | 20660216 |
| microorganisms associated with maari, a baobab seed fermented product. | a microbiological study was carried out on baobab fermented seeds (maari) obtained from 4 different production sites in burkina faso (mansila, toulfé, ouagadougou and gorgadji). a total of 390 representative isolates comprising 251 aerobic mesophilic bacteria (amb) and 139 lactic acid bacteria (lab) were isolated and identified to species level using a combination of pheno- and genotypic methods including conventional morphological analysis, carbohydrate fermentation profiling, rep-pcr ((gtg)(5) ... | 2010 | 20688411 |
| platelet activation and biofilm formation by aerococcus urinae, an endocarditis-causing pathogen. | the gram-positive bacterium aerococcus urinae can cause infectious endocarditis (ie) in older persons. biofilm formation and platelet aggregation are believed to contribute to bacterial virulence in ie. five a. urinae isolates from human blood were shown to form biofilms in vitro, and biofilm formation was enhanced by the presence of human plasma. four of the a. urinae isolates caused platelet aggregation in platelet-rich plasma from healthy donors. the au3 isolate, which induced platelet aggreg ... | 2010 | 20696834 |
| detection of airborne bacteria in a german turkey house by cultivation-based and molecular methods. | today's large-scale poultry production with densely stocked and enclosed production buildings is often accompanied by very high concentrations of airborne microorganisms leading to a clear health hazard for employees working in such environments. depending on the expected exposure to microorganisms, work has to be performed under occupational safety conditions. in this study, turkey houses bioaerosols were investigated by cultivation-based and molecular methods in parallel to determine the conce ... | 2010 | 20720091 |
| molecular analysis of the diversity of vaginal microbiota associated with bacterial vaginosis. | bacterial vaginosis (bv) is an ecological disorder of the vaginal microbiota that affects millions of women annually, and is associated with numerous adverse health outcomes including pre-term birth and the acquisition of sexually transmitted infections. however, little is known about the overall structure and composition of vaginal microbial communities; most of the earlier studies focused on predominant vaginal bacteria in the process of bv. in the present study, the diversity and richness of ... | 2010 | 20819230 |
| molecular cloning, genomic organization and antibacterial activity of a second isoform of antilipopolysaccharide factor (alf) from the mud crab, scylla paramamosain. | antimicrobial peptides (amps) serve a major role in host defense systems against microbial invasion. in this study, a novel isoform (alfsp2) of antilipopolysaccharide factors (alfs) was cloned from the mud crab, scylla paramamosain. the open reading frame of the alfsp2 cdna is 348 bp and encodes for a predicted 115 amino acid residues (12.92 kda), and a mature protein of 94 amino acids and a molecular mass of 10.79 kda. the amino acid sequence of alfsp2 has an overall similarity of 74%, 66% and ... | 2010 | 20883796 |
| streptococcus spp. and related bacteria: their identification and their pathogenic potential for chronic mastitis - a molecular approach. | streptococcus spp. and related bacteria form a large group of organisms which are associated with bovine intramammary infections (imi). some of them are the well-known mastitis pathogens streptococcus uberis and streptococcus agalactiae. in addition, there are a considerable number of these gram-positive, catalase-negative cocci (pnc) with unclear mastitic pathogenicity such as aerococcus viridans which make the conventional diagnostics of pnc difficult. one diagnostic, api 20 strep (api, biomér ... | 2010 | 20971488 |
| first molecular characterization of fluoroquinolone resistance in aerococcus spp. | | 2010 | 21078934 |
| volatile and biogenic amines, microbiological counts, and bacterial amino acid decarboxylase activity throughout the salt-ripening process of anchovies (engraulis encrasicholus). | chemical and microbiological parameters were studied during the industrial production of salt-ripened anchovies (engraulis encrasicholus). gradual acidification and increases in the proteolysis index and in total volatile basic nitrogen were observed. at the end of the maturing process, the values reached ph 5.55 +/- 0.03, 21.33 +/- 5.82%, and 44.06 +/- 12.47 mg/ 100 g, respectively. in the three studied anchovy batches, the biogenic amines tyramine, histamine, putrescine, cadaverine, and agmati ... | 2005 | 21132979 |
| isolation and characterisation of bacterial colonies from seeds and in vitro cultures of fraxinus spp. from italian sites. | culturable bacteria were isolated from seeds, embryos and contaminated in vitro cultures of ash (fraxinus excelsior l., f. ornus l. and f. angustifolia l.) and were identified using morphological and molecular analyses. fourteen morphologically distinct isolates were recovered from seeds of fraxinus spp. 16s rdna sequencing categorised these isolates into ten separate genera. three strains isolated from contaminated in vitro cultures, pantoea agglomerans, staphylococcus succinus and aerococcus v ... | 2011 | 21143738 |
| a case report of successful management of aerococcus urinae peritonitis in a patient on peritoneal dialysis. | | 2010 | 21148062 |
| diseases of american lobsters (homarus americanus): a review. | the american lobster fishery is a significant economic driver in coastal communities of north america. increasingly, the impacts of infectious disease are recognized as important components and factors in the population ecology and subsequent management of the lobster fishery. both environmental and anthropogenic factors impact marine diseases. the review herein highlights aspects of several important bacterial, fungal and protistan diseases, including gaffkemia, shell disease, vibriosis, diseas ... | 2011 | 21215356 |
| two novel antimicrobial peptides, arasin-likesp and grpsp, from the mud crab scylla paramamosain, exhibit the activity against some crustacean pathogenic bacteria. | antimicrobial peptides (amps) are some of the important host molecules required to resist pathogen infection. two novel amps (arasin-likesp and grpsp) were identified from the hemocytes of the mud crab, scylla paramamosain, by analysis of a hemocyte expressed sequence tag library. the deduced open reading frames of the arasin-likesp and grpsp cdnas are 198 and 168 bp, and encode for predicted peptides of 65 and 55 amino acid residues, respectively. the calculated molecular mass of the mature pep ... | 2011 | 21220028 |
| aerococcus viridans urinary tract infection in a pediatric patient with secondary pseudohypoaldosteronism. | aerococcus viridans is a catalase-negative gram-positive bacterium rarely found as human pathogen. some cases of urinary tract infection (uti) have been described in immunocompromised adults. in this article we describe a uti case caused by this agent in a child with severe obstructive uropathy, clinically presented with secondary pseudohypoaldosteronism (spha). although a. viridans is rarely associated with child infection, it can be responsible for life threatening conditions/ situations. to o ... | 2010 | 21229195 |
| an unusual microorganism, aerococcus viridans, causing endocarditis and aortic valvular obstruction due to a huge vegetation. | aerococcus viridans is not a common pathogen, and endocarditis due to a. viridans is very rare. a 44-year-old woman with persistent atrial fibrillation and rheumatic valvular heart disease was admitted with fever, sweating, weakness, and progressive shortness of breath. transthoracic echocardiography (tte) demonstrated a 8x9-mm vegetation attached to the right coronary cusp of the aortic valve, causing aortic obstruction. blood cultures yielded a. viridans susceptible to penicillin. despite opti ... | 2011 | 21646834 |
| [aerococcus urinae infective endocarditis.] | | 2011 | 21794855 |
| clinical significance and antimicrobial susceptibilities of aerococcus sanguinicola and aerococcus urinae. | a retrospective chart review was performed on 92 patients from whom 118 isolates of aerococcus sanguinicola (n = 52) or aerococcus urinae (n = 66) were obtained from urine cultures between october 2007 and june 2008 to assess clinical presentation and antimicrobial susceptibilities. the mean patient age was 82 (range 24-101) years. the majority was female (76% and 87% for a. sanguinicola and a. urinae, respectively) and institutionalized (61% and 60%, respectively). the majority of male patients ... | 2011 | 21767701 |
| phenotypic characterisation and 16s rrna sequence analysis of veterinary isolates of streptococcus pluranimalium. | forty-two isolates of streptococcus pluranimalium were identified from cattle (n=38), sheep (n=2), an alpaca (n=1) and a pheasant (n=1) in the united kingdom. the isolates were confirmed as s.pluranimalium by 16s rrna sequence analysis but could not be differentiated reliably from streptococcus acidominimus by phenotypic characterisation using commercial kits routinely used in veterinary laboratories. the alanyl-phenylalanyl-proline arylamidase reaction could be used to differentiate s. pluranim ... | 2011 | 21703886 |
| culture-negative infective endocarditis of the aortic valve due to aerococcus urinae: a rare aetiology. | bacteria of the species aerococcus urinae are gram-positive, catalase-negative cocci that are arranged in pairs, tetrads, or clusters resembling enterococci or staphylococci. they are rare causative agents of infective endocarditis. repetitive urinary tract infections based upon underlying genitourinary tract abnormalities could involve these bacteria. due to their similarity to other gram-positive cocci misinterpretation may occur along the line of microbiologic differentiation, which could pot ... | 2011 | 21889909 |
| Successful treatment of Aerococcus viridans endocarditis in a patient allergic to penicillin. | Aerococcus viridans is a rare human pathogen that occasionally causes endocarditis. Most of the reported cases of endocarditis have been treated with penicillin. Here we describe a patient who was allergic to penicillin and was successfully treated with cefotaxime. | 2011 | 22154998 |
| aerococcus viridans expression of cpn60 is associated with virulence during infection of the american lobster, homarus americanus milne edwards. | the gram-positive bacterium aerococcus viridans var. homari is a well-documented causative agent of the lethal systemic disease gaffkemia in both the american lobster, homarus americanus, and the european lobster, homarus gammarus. previous phenotypic characterization has been unsuccessful at differentiating avirulent from virulent strains without performing lethal animal infection trials. recent genetic characterization of a. viridans strains through 16s rrna sequencing and random amplification ... | 2011 | 21988355 |
| evaluation of microorganisms cultured from injured and repressed tissue regeneration sites in endangered giant aquatic ozark hellbender salamanders. | investigation into the causes underlying the rapid, global amphibian decline provides critical insight into the effects of changing ecosystems. hypothesized and confirmed links between amphibian declines, disease, and environmental changes are increasingly represented in published literature. however, there are few long-term amphibian studies that include data on population size, abnormality/injury rates, disease, and habitat variables to adequately assess changes through time. we cultured and i ... | 2011 | 22205979 |
| cow teat skin, a potential source of diverse microbial populations for cheese production. | the diversity of the microbial community on cow teat skin was evaluated using a culture-dependent method based on the use of different dairy-specific media, followed by the identification of isolates by 16s rrna gene sequencing. this was combined with a direct molecular approach by cloning and 16s rrna gene sequencing. this study highlighted the large diversity of the bacterial community that may be found on teat skin, where 79.8% of clones corresponded to various unidentified species as well as ... | 2012 | 22081572 |
| aerococcus urinae and trimethoprim-sulfamethoxazole. | aerococcus urinae has been described as resistant to trimethoprim-sulfamethoxazole (sxt), but the test medium may affect this observation. twenty-seven clinical isolates of a. urinae tested susceptible to sxt in cation-adjusted mueller-hinton broth (camhb) plus lysed horse blood and resistant in camhb plus lysed sheep blood. | 2011 | 21918023 |
| Which aerococcus? comment on: an unusual microorganism, aerococcus viridans, causing endocarditis and aortic valvular obstruction due to a huge vegetation (turk kardiyol dern ars 2011;39:317-9). | | 2011 | 21983782 |
| Clinical and microbiological features of bacteraemia with Aerococcus urinae. | Clin Microbiol Infect ABSTRACT: Aerococcus urinae is a Gram-positive bacterium that can cause invasive infection, including infectious endocarditis (IE), mainly in older men. A. urinae is often misclassified in routine diagnostic laboratories. Through searches in the laboratory databases we identify 16 isolates of A. urinae causing bacteraemia during a 6-year period in southern Sweden, indicating that bacteraemia with A. urinae occurs in at least three cases per million inhabitants per year. The ... | 2011 | 21895858 |
| culture-independent exploration of the teat apex microbiota of dairy cows reveals a wide bacterial species diversity. | due to their close proximity to the mammary gland tissue, the bacterial communities lining the teat apex of the udders from lactating cows influence udder health. denaturing gradient gel electrophoresis of the amplified v3 variable region of the 16s rrna gene was used as a culture-independent method to reveal the bacterial composition of 48 samples originating from the teat apices of twelve friesian-holstein dairy cows suffering from clinical mastitis in one quarter. the microbiota belonged to f ... | 2011 | 22266158 |
| [a case of infective aerococcus urinae endocarditis successfully treated by aortic valve replacement]. | aerococcus urinae is a endocarditis rare causative organism with low virulene. we report an a. urinae endocarditis case treated by aortic valve replacement. an 80-year-old woman hospitalized for urinary tract infection and hydronephrosis due to three-week renal calculi. blood culture on admission isolated streptococcus acidominimus. during the course, she was transferred to our care for surgical intervention after developing congestive heart failure due to severe aortic regurgitation. echocardio ... | 2011 | 22250461 |