a reproducible method for reading folate assays with observations on perinatal changes in tissue folate concentration. 1975238032
the inhibition of bacterial growth by ochratoxin a.a series of bacterial species was examined for their sensitivity to ochratoxin a. only grampositive bacteria could be inhibited, generally at a ph lower than 7.0. bacillus subtilis did not show any reduction of growth rates in presence of ochratoxin a, but had a prolonged lag phase. with staphylococcus pyogenes var. aureus and streptococcus faecalis, a prolonged lag phase and a reduction of the growth rate was observed. most sensitive was streptococcus faecalis in the exponential-growth phase. t ...1975238733
purification and properties of an nadp-specific 6-phosphogluconate dehydrogenase from streptococcus faecalis.a procedure is described for the purification of 6-phosphogluconate dehydrogenase (6-phospho-d-gluconate:nadp oxidoreductase (decarboxylating) ec from cell extracts of streptococcus gaecalis. a 180-fold purification was achieved with an over-all yield of about 12% and an average specific activity of 14. the enzyme was homogeneous as determined by polyacrylamide gel electrophoresis, immunoelectrophoresis, and sedimentation equilibrium, studies. its weight average molecular weight, as me ...1975238996
comparative inhibitory activity of bl-s640 and two other vitro antibacterial activity of bl-s640 was compared to that of cephalothin and cephalexin against gram-negative and gram-positive bacteria isolated from clinical specimens. bl-s640 demonstrated the best activity on nearly all microbial species studied, except for haemophilus influenzae and diplococcus pneumoniae against which cephalothin was slightly more active.1975239917
studies of introital colonization in women with recurrent urinary infections. i. the role of vaginal ph.the ph of the vaginal introitus was compared to quantitative cultures of the introital mucosa (escherichia coli, proteus mirabilis and streptococcus faecalis) in 800 samples. introital carriage of more than 100 bacteria per ml. was significantly greater at ph of more than 4.4 (p less than 0.005) when compared to mucosal ph of less than or equal to 4.4.1975240038
synthesis and antibacterial properties of methylsulfinyl and methylsulfonyl analongs of some nitrofurans.the sulfoxides 5-methylsulfinyl-2-furaldehyde semicarbazone (2) and 1-[(5-methylsulfinyl-2-fufurylidene)amino]hydantoin (3) as well as the sulfones 1-[(5-methylsulfonyl-2-furfurylidene)animo]hydantoin (1) and 1-(5-methylsulfonly-2-furyl)-2-(6-amino-3-p-ridazyl)ethylene hydrochloride (4) have been prepared and tested for antibacterial activity against a number of gram-negative and gram-positive organisms. the compounds are much less active than the corresponding 5-nitrofuran derivatives, possibly ...1975240940
antibacterial activity of cationic proteins from human granulocytes.human granulocytes contain several cationic proteins with a molecular weight of approximately 25,000, almost identical amino acid composition, and complete immunologic identity. these proteins possess a chymotrypsin-like protease activity at a neutral ph. the antibacterial activity of the cationic proteins has been studied. bactericidal activities are found against both gram-positive (streptococcus faecalis and staphylococcus aureus) and gram-negative (escherichia coli and pseudomonas aeruginosa ...1975241758
antimicrobial activity of human peritoneal fluid.cell free peritoneal fluids from 23 normal women had antimicrobial activity which was directed against gram-positive and gram-negative bacteria and also against yeasts. the activity was bacteriostatic or bactericidal and seemed to be due to the presence of a variety of factors which varied in their heat stability and dependence on complement. lysozyme was present in the fluids but was not responsible for all the antimicrobial activity.1975242084
resistance to six aminoglycosidic aminocyclitol antibiotics among enterococci: prevalence, evolution, and relationship to synergism with penicillin.two hundred and three recent clinical isolates of enterococci were tested for susceptibility to streptomycin, kanamycin, amikacin, gentamicin, sisomicin, and tobramycin. depending upon the source of the isolate, 36 to 54% of the enterococci demonstrated high-level resistance (minimal inhibitory concentration, >2,000 mug/ml) to streptomycin, 16 to 49% to kanamycin, and 0 to 14% to amikacin. none of the strains was highly resistant to gentamicin, sisomicin, or tobramycin. a comparison with isolate ...1977242911
comparative activities of ampicillin, epicillin and amoxycillin in vitro and in vivo.the antibacterial activities of three aminopenicillins ampicillin, epicillin and amoxycillin were compared in vitro and in vivo. the minimum inhibitory concentrations (mic) of the three penicillins were very similar and the compounds were active against non-beta-lactamase-producing strains of escherichia coli, salmonella and shigella species, proteus mirabilis, haemophilus influenzae and neisseria gonorrhoeae. streptococci including streptococcus faecalis, and non-beta-lactamase-producing staphy ...1979256524
"conjugal" transfer of plasmid dna among oral streptococci.the beta plasmid from streptococcus faecalis strain ds5, which codes for resistance to erythromycin and lincomycin, was introduced into a lancefield group f streptococcus, strain dr1501, by transformation. this strain, dr1501 (beta), was found to be an excellent donor of the beta plasmid and readily transferred the resistance markers to various lactic acid bacteria, including certain strains of s. mutans, s. sanguis, and s. salivarius. evidence is presented indicating that the transfer of the be ...1978277950
in-vitro activity of cefoxitin against recent clinical bacterial isolates.the susceptibility of 635 clinical isolates of bacteria to cefoxitin was determined. isolates from the urinary tract showed 97% sensitivity, isolates from infected wounds showed 93% sensitivity and 82% of blood culture isolates were sensitive. streptococcus faecalis isolates showed only 50% sensitivity and pseudomonas organisms were predominantly resistant. bacteria isolated from unprepared heart valves submitted for use as homografts showed only 71% sensitivity due to the large proportion of st ...1979286213
effect of intestinal bacteria on incidence of liver tumors in gnotobiotic c3h/he male mice.the effect of intestinal microflora on liver tumorigenesis was studied in gnotobiotic c3h/he male mice monoassociated, diassociated, or polyassociated with the following strains of intestinal bacteria: escherichia coli, streptococcus faecalis, bifidobacterium adolescentis, bifidobacterium infantis, clostridium indolis, c. paraputrificum, c. perfringens, c. innocuum, c. nexile, c. ramosum, c. clostridiiforme, bacteroides multiacidus, bacteroides fragilis, veillonella alcalescens, v. parvula, and ...1979292809
lower urinary tract pathogens in the dog and their sensitivity to chemotherapeutic agents.ninety-six urine samples were collected by a sterile technique from 75 dogs affected with urinary tract disease (cystitis, urolithiasis, prostatitis, etc) involving bacteruria. the infecting organisms were isolated and tested against sensitivity discs (penicillin g, streptomycin, chloramphenicol, tetracycline, sulphamethoxazole/trimethaprin and sulphatriad). the commonest isolate was escherichia coli, which was generally sensitive to several agents, though in eight cases it was resistant to all ...1977331657
qualitative study of paradoxical zone phenomenon of penicillins against 17 bacterial species of clinical importance.using a triple agar layer technique and enzymatic inactivation of penicillin, the occurrence of a paradoxical zone phenomenon (illustrated by a typical 'target' image around the reservoir of antibiotic) was determined for several bacterial species specially chosen with regards to their taxonomic position, clinical importance and penicillin susceptibility. among gram-positive bacteria, a paradoxical zone was obtained for approximately 43% of the strains studied here (all 10 strains of staphylococ ...1978340171
antibacterial activity of cefamandole in vitro.cefamandole, a new cephalosporin derivative, was found to have a broad spectrum of antimicrobial activity against a cross-section of both gram-positive and gram-negative bacteria isolated clinically. gram-positive cocci, except for streptococcus faecalis, were extremely susceptible to cefamandole; penicillin g-resistant staphylococcus aureus also was highly susceptible. minimal bactericidal concentrations for gram-positive cocci approximated the minimal inhibitory concentrations. strains of haem ...1978349095
folate antagonists. 10. synthesis and antimalarial effects of 6-[[(aryl and aralkyl)amino]methyl]-2,4-pteridinediamines and -pteridinediamine 8-oxides.various 6-[[(aryl and aralkyl)amino]methyl]-2,4-pteridinediamines and their 8-oxides have been synthesized for antimalarial evaluation. condensation of 3-amino-6-(bromomethyl)-2-pyrazinecarbonitrile 4-oxide (v) with the appropriately substituted amine afforded a series of 3-amino-6-[[(aryl and aralkyl)amino]methyl]-2-pyrazinecarbonitrile 4-oxides vi. deoxygenation gave the corresponding pyrazines vii. cyclization of vi and vii with guanidine then produced the desired 6-(aminomethyl)-2,4-pteridin ...1978349157
a comparison of the in vitro activity of a new cephalosporin, cefuroxime, and cephalothin against 810 clinical isolates.810 strains of eight species: staphylococcus aureus, staphylococcus epidermidis, streptococcus faecalis, escherichia coli, klebsiella sp, enterobacter sp, proteus mirabilis and proteus vulgaris, were tested by the disc diffusion method against cephalothin and cefuroxime. the activity of the two drugs was comparable to s aureus and s epidermidis, and a little in favour of cefuroxime regarding s faecalis. cefuroxime turned out to be superior to cephalothin against the gram negative rods investigat ...1979398460
[the sulfamethoxazole-trimethoprim combination 1st survey into its in vitro activity in brazzaville, congo results of 780 bacterial strains isolated at the national laboratory and the general hospital in a single year].the combination of smz-tmp has recently been introduced in congo. this year (1978), it is very used by many physicians. to help the congolese physicians working without antibiogram frequently, we have tested 780 strains from august 1977 to august 78. the susceptibility of these strains was: for e. coli 90%, klebsiella 69,20%, staphylococcus 93% and for all strains studied gram + and gram - the percentage was 75,51. as many authors have showed, the strains of pseudomonas, streptococcus faecalis a ...1979400121
in vitro antimicrobial activity of some cyclic hydroxamic acids and related lactams.against enterobacter aerogenes 13048, serratia marcescens 13880, klebsiella pneumoniae 10031, pseudomonas aeruginosa 10145, escherichia coli 9723, lactobacillus casei 7469, lactobacillus plantarum 8014, leuconostoc dextranicum 8086, and streptococcus faecalis 8043, the mean minimal inhibitory concentrations of three cyclic hydroxamic acids, 3-amino-3,4-dihydro-1-hydroxycarbostyril, the 6-chloro analog, and the 7-chloro analog, were 0.6, 0.6, and 0.2 micrograms/ml, and those of the corresponding ...1978400828
identification and characterization of a small sequence located at two sites on the amplifiable tetracycline resistance plasmid pamalpha1 in streptococcus faecalis.streptococcus faecalis dt-11 harbors the 6.0-megadalton plasmid pamalpha1, which determines resistance to tetracycline (tc). when this strain is grown in the presence of tc for a number of generations, a reversible gene amplification occurs, generating tandem repeats of a 2.65-megadalton segment of the plasmid. on the basis of heteroduplex studies between various forms of pamalpha1 and fragments generated by the escherichia coli restriction endonuclease ecori, we have obtained direct evidence fo ...1977401496
collaborative study of turbidimetric microbiological assay of menensin in chicken rations. 1977401805
anti-glycosyl antibodies: resolution of anti-gal isoantibodies into individual components by electrofocusing. 1977402139
selective electrode for dibenzyl dimethyl ammonium cation as indicator of the membrane potential in biological systems.the electrode sensitive to dibenzyl dimethyl ammonium (dda+), which is considered to be an indicator of the membrane potential, was constructed by using tetraphenyl borone (tpb-) embedded in dichloroethane. rapid and nernstian responses were exhibited against dda+ solutions ranging between 10(-2) and 3 - 10(-6) m in concentration. high selectivity for dda+ was observed in the presence of various inorganis salts, adp, atp, oxidizable substrates and sugars. the electrode developed here was used to ...1977402152
thermostable inhibitor(s) of pyruvate dehydrogenase complex from streptococcus faecalis. 1977402292
the amino acid sequence of dihydrofolate reductase from l1210 cells. 1977402293
sorption of streptococcus faecium to glass.a method has been developed by which to study the sorption of streptococcus faecium to soda-lime cover glasses. conditions were chosen to minimize the influence on sorption of bacterial polymer production, passive sorption being studied rather than attachment mediated by metabolic activities. sorption of s. faecium increased with increasing temperature (to 50 degrees c), time, and cell concentration, but equilibrium apparently was not reached even after incubation for 8 hours or at a cell concen ...1977402789
sorption of streptococci to glass: effects of macromolecular solutes.macromolecular solutes (albumin, concanavalin a, whole saliva, serum) caused impaired sorption of streptococcus faecium and streptococcus sanguis to glass. the inhibitory effect resided chiefly with interactions of the solutes with the glass surface. in the case of sorption of s. sanguis to glass in the presence of parotid fluid, the inhibitory effect was counteracted by a specific attachment of s. sanguis cells mediated by some component(s) of the parotid fluid. agglutination of the test organi ...1977402790
[oral treatment of enterococcal endocarditis in prolapsed mitral valve]. 1977403109
radiometric detection of bacteremia: requirement for terminal subcultures.a radiometric procedure for rapid detection of bacteria in clinical blood specimens was utilized over a period of 1 year in this laboratory. although in initial studies it was felt that all positive bacteremias would be detected by radiometric examination of cultures for 14co2 evolution over a 7-day period, we found in the present study that a significant number of bacteria were not detected, except by blind subculturing on day 7 before discarding the culture sample. microorganisms were detected ...1977403198
[the spontaneous external biliary fistula]. 1977403476
[opsonizing activity of the serum of gnotobiotic guinea pigs contaminated with individual representatives of intestinal microflora].the influence of contamination of germfree guinea pigs with individual representatives of the intestinal microflora (bac. mesentericus, bac. subtilis, s. albus, and s. faecalis) on the formation of the serum opsonic activity was studied. an increase of the opsonic activity to all the microorganisms on the 11th day after a corresponding monocontamination and a stimulating influence of the serum on the intracellular digestion of bac. mesentericus and bac. subtilis microbes was noted. as to the pat ...1977403978
[reservoir for bacteria of urinary tract infections in urologic patients (author's transl)].many infections of the urinary tract, especially after surgical treatment are nosocomial infections. the etiological bacteria could only be found in the urine of the infected patients and in the faecal flora of nearly 40% of all stationary patients. the so called "problem bacteria": klebsiella, proteus rettgeri, pseudomonas showed increasing drug resistance during the observed period between 1972-1975. the bacteria isolated from the faecal flora also showed multiple drug resistance. thus we conc ...1977404234
comparative studies on the sampling and investigation of microbial contamination of surfaces by the contact plate and swab methods. 1977404279
evaluation of the rapid hippurate hydrolysis test with enterococcal group d streptococci.the rapid hippurate hydrolysis test was evaluated with the conventional test, using 17 group a streptococci, 9 non-enterococcal group d streptococci, 108 enterococcal group d streptococci, and 2 strains of listeria monocytogenes. there was complete correlation between the rapid and conventional tests with all organisms except enterococcal group d. the rapid hippurate hydrolysis method was more sensitive with the enterococci; 95.4% were positive with the rapid method, and 9.3% were positive with ...1977404312
aminoglycoside-resistant enterococci.thirty-four recent clinical isolates of streptococcus faecalis were tested for sensitivity to amoxycillin, benzylpenicillin, streptomycin, kanamycin, gentamicin, tobramycin, and amikacin. amoxycillin was two- to four-fold more active than benzylpenicillin and all strains were inhibited by low concentrations of the penicillins. the aminoglycosides were less active against the enterococci than were the penicillins and a significant number of strains were insensitive or relatively insensitive to on ...1977404337
evidence that the multi-drug-resistance of streptococcus faecalis bio-4r is not carried by a plasmid.the multi-drug-resistant strain streptococcus faecalis bio-4r was studied to see if it carried a plasmid responsible for antibiotic resistance. from results indicating that the antibiotic resistance of s. faecalis bio-4r was not transferred to recipient bacteria, that the organism did not produce enzymes which inactivated antibiotics, and that the presence of covalently closed circular dna was not demonstrated by dye-cesium chloride buoyant density gradient centrifugation, it was concluded that ...1977404504
[anatomic and bacteriological study of surgically extracted valves in patients with bacterial endocarditis].we studied 64 cases of bacterial endocarditis which required heart surgery for hemodynamic defects and/or failure of the antibiotic therapy. we performed a bacteriologic and histologic investigation of the removed valves. the organisms were evidenced through culture and specific stainings. according to the different organisms we evaluated the best duration of antibiotic therapy to achieved sterilization, and suggest a helpful medical and surgical treatment for the bacterial endocarditis.1977404630
[dynamics of the establishment of intestinal microflora in healthy full-term newborns].a study was made of the dynamics of establishment of the intestinal microflora in 79 healthy mature neonates born of healthy mothers. a total of 223 bacteriological examinations were conducted. a stable prevalence of bifidobacteria over the aerobic portion of the intestinal biocenosis occurred in all the children from the 8th day after birth. along with this the following bacteria were continuously present in the intestine: lactic bacilli and streptococci e. coli, enterococci and staphylococci; ...1977404808
antibacterial effects of metrizoate and metrizamide on bacterial growth in vitro.urinary pathogens were exposed in vitro to isopaque and amipaque in concentrations of 100 mg i/ml and 260 mg i/ml. both contrast media in the higher concentration had a slight or negligible bacteriostatic effect on some of the test bacteria. no bactericidal effect was detected. consequently, radiography of the urinary tract with these two media in the concentrations mentioned does not interfere with the culturing of bacteria from urine samples.1977404846
antimicrobial systems of the surgical wound. ii. detection of antimicrobial protein in cell-free wound fluid.human wound fluid contains heat-stable proteins with moderate antibacterial activity against staphylococcus aureus and escherichia coli, and different heat-labile proteins with antibacterial activity against e coli. blood serum also contains heat-labile antibacterial substances, but little heat-stable activity against staphylococcus aureus. both blood serum and wound fluid have bacteriostatic activity against s epidermidis, and early growth of streptococcus fecalis occurs in serum and wound flui ...1977404931
neonatal anaerobic (clostridial) cellulitis and omphalitis. 1977404971
interactions between beta-lactam antibiotics and isolated membranes of streptococcus faecalis atcc 9790.the dd-carboxypeptidase-exchange membrane-bound enzyme in streptococcus faecalis atcc 9790 reacts with beta-lactam antibiotics to form complexes with rather long half-lives. depending upon the antibiotic, the second-order rate constants for complex formation range from 0.75-560 m-1 s-1 (at 37 degrees c and in water) and the first-order rate constants for complex breakdown range from 1.3 to 26 x 10(-5) s-1 (at 37 degrees c and in 5 mm phosphate buffer ph 7.5). there are about 30 pmol of dd-carbox ...1977405216
bacteriological studies in a patient with antibiotic-associated pseudomembranous colitis.detailed aerobic and anaerobic bacteriological studies on the operative specimen from a patient with antibiotic-associated pseudomembranous colitis revealed approximately 10(11) facultatively anaerobic bacteria, but less than 10(5) obligate anaerobes per g of involved colonic tissue. fourteen isolates of escherichia coli, three isolates of pseudomonas aeruginosa, and two isolates of streptococcus fecalis were identified. the majority of the organisms were resistant to most of the antibiotics tha ...1977405269
feedback inhibition of the synthesis of an antibiotic: aurodox (x-5108).the effect of aurodox on its own biosynthesis by streptomyces goldiniensis was studied. it was found that addition of exogenous aurodox inhibits further accumulation of aurodox by the antibiotic-producing culture. both long term fermentation studies with aurodox-14c and precursor incorporation studies over short time periods indicated that aurodox synthesis was regulated by feedback inhibition. the concentration of aurodox required to completely block further synthesis of the antibiotic was abou ...1977405357
genetic loci of hemolysin production in streptococcus faecalis subsp. zymogenes.two plasmids corresponding to molecular weights of 38.5 x 10(6) and 3.6 x 10(6) have been identified in streptococcus faecalis subsp. zymogenes strain x-14. the larger plasmid is required for hemolysin-bacteriolysin production. strain l2, a nonlytic nitrosoquanidine mutant of strain x-14, still harbors the hemolytic plasmid and produces the lysin component, but not the activator component, of the lytic system. conjugal transfer of this plasmid from strain l2 to plasmid-free strains and strains c ...1977405371
serum folates in an aseptic microbiological assay of folate compounds and their breakdown compounds, using lactobacillus casei, streptococcus faecalis, and pediococcus cerevisiae, 4a-hydroxy-5methyl-4,5,6,7-tetrahydrofolate and 5-methyl-5,8-dihydrofolate were inactive under all conditions to all three organisms and 5-methyl-5,6-dihydrofolate was inactive unless ascorbate was present in the incubation medium, and then only to l. casei. 5-methyltetrahydrofolate was active only for l. casei, and activity in puri ...1977405403
an investigation of the bacteriological quality of retail vanilla hundred and thiry-three vanilla slices, purchased from shops in the west yorkshire metropolitan county, were examined to determine the numbers and types of bacteria present at the time of purchase. the surface colony count at 37 degrees c was greater than 10(3)/g in 67/133 (50%) of the samples examined, bacillus cereus being found at that concentration in 21.8%, coliform bacilli including e. coli in 5.3%, staphylococcus aureus in 3-0% and streptococcus faecalis in 0-8%. thirty-four strains o ...1977405420
[chemical, pharmacological and clinical study on conventional and current chewing gums. iv. microbiological control of chewing gums]. 1977405917
energetics of streptococcal growth inhibition by hydrostatic pressure.growth of streptococcus faecalis in complex media with various fuel sources appeared to be limited by the rate of supply of adenosine-5' -triphosphate (atp) at 1 atm and also under 408 atm of hydrostatic pressure. growth under pressure was energetically inefficient, as indicated by an average cell yield for exponentially growing cultures of only 10.7 g (dry weight) per mol of atp produced compared with a 1-atm value of 15.6. use of atp for pressure-volume work or for turnover of protein, peptido ...1977405925
ribose biosynthesis in streptococcus mutans. 1977405960
bacteraemia in asymptomatic human subjects. 1977405961
chemotherapy of experimental streptococcal endocarditis. vi. prevention of enterococcal endocarditis. 1977406336
a pure enterococcal abscess after cesarean section.the pathogenic role of the enterococcus in pelvic infections is controversial. this case report of a pure enterococcal abscess developing after cesarean section supports the view that this organism can be a primary pathogen in obstetric and gynecologic infections. because of its unusual sensitivity pattern, the enterococcus requires specific antibiotic therapy.1977406387
[identification of pathogenic streptococci. grouping by co-agglutination]. 1977406597
rapid microbiological assay for chlorhydroxyquinoline that uses a cryogenically stored inoculum.a rapid microbiological assay for chlorhydroxyquinoline is described. it is a turbidimetric procedure that uses streptococcus faecalis as the test organism. results are available within 5 h. data are presented to show the advantages of using a cryogenically stored inoculum over an inoculum prepared on a daily basis.1977406830
evaluation of a new presumptive medium for group d streptococci.a new medium designated as d streptococcus-enterococcus broth was formulated and evaluated for the enrichment and isolation of strains of serological group d streptococci. this medium was made by modifying todd-hewitt broth. most-probable-number multiple-tube and membrane filter techniques were employed to estimate the numbers of enterococci in known cultures, wastewater, and other samples. preliminary most-probable-number counts with this medium were as much as 3 logs higher than those counts o ...1977406842
detection and microbiologic assays of antimetabolites.the biochemical considerations applicable in the detection of new antimetabolites are very similar to those used in developing microbiologic assays for these drugs. an in vitro system for detection of antimetabolites was developed based on different sensitivities to such drugs of bacteria cultivated in complex versus completely synthetic media. a number of new antimetabolites of amino acids, vitamins, and purines or pyrimidines were isolated and microbiologic assays for these drugs were develope ...1977406992
efficacy of ammoniacal silver nitrate in root canal order to find a simple and effective method for endodontic treatment of recalcitrant cases, the efficacy of ammoniacal silver nitrate was re-evaluated. the modified serial tube dilution method was conducted to determine the effective antimicrobial concentration of ammoniacal silver nitrate on selected test microorganisms and clinical isolates. the effective antimicrobial concentration for the most resistant microorganism, streptococcus faecalis, was 5.0 x 10(-10) g/ml and the effectiveness pe ...1977407015
effect of a quinazolinone derivative on the metabolism of streptococcus faecalis--r (atcc-8043).2-methyl-3(3'-methyl-2'-pyridyl)-4 (3h) quinazolinone (src-820) and methaqualone inhibited the growth of streptococcus faecalis-r (atcc-8043). phosphoenolpyruvate formation from 3-phosphoglycerate by an enzyme extract of s. faecalis was inhibited by src-820. with edetate or fluoride, an additive inhibitory effect by src-820 was observed.1977407153
[preliminary tests of the influence of wastewaters from sugar-factories upon the numbers of intestinal bacteria in water (author's transl)].this work presents the results of experiments dealing with the influence of wastewaters from 2 sugar factories in the czechoslovakia upon some representatives of intestinal bacteria: e. coli, c. feundii, e. aerogenes, s. anatum, s. schottmuelleri, s. typhi-murium, sh. sonnei and str. faecalis. the experiments were carried out at 30, 20 and 10 degrees c. the experiments have shown that the presterilized waste-water from sugar factory (in bohemia and in slovakia) caused rapid multiplication of the ...1977407746
methodological aspects of an agar plate technique for determination of biologically active 5-fluorouracil in blood, urine and bile.a microbiological agar plate technique for estimation of 5-fluorouracil concentrations in blood, urine and bile from man, dog and pig was evaluated. different bacterial test strains, media modifications and techniques for inoculation were studied. the strain streptococcus faecalis atcc 8043, recommended previously by clarkson et al., was found to be the most suitable. the influence of prediffusion, dilution, antibiotics and chemotherapeutic agents and their antagonists, as well as the effect of ...1977407775
adults with chronic granulomatous disease of "childhood". 1977407791
folic acid compounds in romaine lettuce.the composition of folate coenzymes in romaine lettuce was studied. lettuce extract was purified on qae-sephadex a-25 and folate compounds were separated into a monoglutamate fraction and a polyglutamate fraction by chromatography on sephadex g-15. both the mono- and poly-glutamate fractions were resolved on deae-cellulose. positive identification of deae peaks was made by further cochromatography with high specific activity radioactive marker folate compounds and with differential microbiologic ...1977407992
immune response and dental caries incidence in streptococcus faecalis-monoassociated harvard caries-resistant and caries-susceptible rats.harvard caries-resistant rats (cr) monoassociated with cariogenic streptococcus faecalis (sf) for 150 days developed early high levels of specific sf serum agglutinins, moderate levels of salivary antibody, and little or no caries activity. harvard caries-susceptible (cs) sf-monoassociated rats developed serum antibody more slowly and never reached the titers of the cr group. little or no salivary agglutinin was found, and extensive caries developed. second-generation monoassociated cr rats (cr- ...1977408270
serum dilution test for bactericidal activity. ii. standardization and correlation with antimicrobial assays and susceptibility tests. 1977408424
quantitative measurement of the bactericidal capability of neutrophils from patients and carriers of chronic granulomatous disease.previous studies examined the ability of polymorphonuclear leukocytes (pmn) from patients and carriers of chronic granulomatous disease (cgd) to kill small numbers of bacteria in vitro. the purpose of the present study was to accurately measure the number of microorganisms killed by cgd pmn when the initial number of bacteria per neutrophil was increased. bactericidal capacities of pmn from seven patients and five proven carriers of cgd were examined with five test ratios ranging from 1.25 to 10 ...1977408453
inhibition of different serotypes of listeria monocytogenes by enterocins in solid and liquid media.twenty-one enterocinogenic strains of enterococci were examined for their ability to inhibit 51 strains of listeria monocytogenes belonging to eight different serotypes; 50 strains of l. monocytogenes were uniformly inhibited on solid media and in broth cultures by strains of streptococcus faecium. however, only four strains of l. monocytogenes were inhibited by strains of streptococcus faecalis, s. faecalis v. zymogenes or s. faecalis v. liquefaciens. enterocin e1a prepared from the supernant f ...1977408493
studies of introital colonization in women with recurrent urinary infections. viii. the role of bacterial interference.the non-pathogenic aerobic bacteria in 97 quantitative introital cultures from 11 women with documented recurrent bacteriuria were compared statistically to those in 100 quantitative introital cultures from 10 control women with no history of bacteriuria. the colonization incidence of the non-pathogenic organisms was not statistically different for the 2 groups, except for gamma hemolytic streptococcus (p equals 0.002). no strongly significant correlations were demonstrated between the colonizat ...1977408518
association of streptococcus bovis with carcinoma of the colon.two patients with colonic adenocarcinoma and streptococcus bovis endocarditis suggested a possible association between the two. non-enterococcal group d streptococci were isolated from fecal cultures of 11 of 105 controls, 35 of 63 patients with carcinoma of the colon, seven of 25 with inflammatory bowel disease, four of 21 with non-colonic neoplasms and five of 37 with other gastrointestinal disorders. all such streptococci examined for lactose fermentation were s. bovis. the prevalence of s. b ...1977408687
[comparison between the activity of various cephalosporins against staphylococci and streptococci group d in vitro]. 1977408803
[proton permeability of streptococcus faecalis membranes and rat liver submitochondrial particle membranes following irradiation]. 1977408869
transferable plasmids mediating multiple-antibiotic resistance in streptococcus faecalis subsp. strain hk187 (streptococcus faecalis subsp. liquefaciens) four plasmids were isolated. the molecular weights, as analyzed in neutral sucrose gradients and by electron microscopy, were found to be about 36 x 10(6), 31 x 10(6), 26 x 10(6), and 4 x 10(6). plasmid 3, also designated pfk14, with a molecular weight of 26 x 10(6), was found to be responsible for resistance to chloramphenicol and erythromycin and also for high-level resistance to streptomycin and lincomycin. in mixed cultures these f ...1977409342
temperature effects on minimum inhibitory and bactericidal concentrations of cell wall antibiotics in streptococcus faecalis.the minimal inhibitory and bactericidal concentrations were determined at 30 and 37 degrees c for several antibiotics affecting cell wall biosynthesis. the test organism, streptococcus faecalis atcc 9790, possesses a single autolytic enzyme and does not produce penicillinase. penicillin, methicillin, and, possibly, bacitracin appeared more effective at 37 than at 30 degrees c. vancomycin, ristocetin, and phosphonomycin appeared equally effective at both temperatures, and cycloserine appeared con ...1977409344
search for a causal agent of psoriasis by seroepidemiologic methods. 1977409353
heterogeneity of nonimmune immunoglobulin fc reactivity among gram-positive cocci: description of three major types of receptors for human immunoglobulin g.two hundred and thirty strains of various gram-positive cocci were tested for quantitative, nonimmune binding of radiolabeled human polyclonal immunoglobulin g (igg). the majority of coagulase-positive staphylococci and streptococci belonging to serogroups c and g showed a high uptake of igg. the binding of immunoglobulin to group a streptococci was considerably less, with a number of strains completely negative. none of the pneumococcal or the group b or d streptococcal strains displayed any bi ...1977409673
processing of streptococcal cell walls by rat macrophages and human monocytes in vitro.phagocytosis and degradation of cell walls by peritoneal macrophages obtained from fischer 344 or buffalo rats was measured in tissue culture. group a cell wall antigen, detected by immunofluorescence, persisted in cultured rat macrophages for at least 40 days, whereas group d cell wall material was eliminated by 6 to 8 days. this same pattern of persistence of group a cell walls and elimination of group d cell walls was observed in cultures of human monocytes followed for 24 days in culture. gr ...1977409678
cytotoxicity of rat macrophages activated by persistent or biodegradable bacterial cell walls.peritoneal macrophages from fischer 344 rats were cytotoxic for mouse fibroblasts after in vitro phagocytosis of non-biodegradable group a streptococcal cell walls. in contrast, macrophages from buffalo rats were not activated to cytotoxicity by bacterial cell walls. activation to cytotoxicity was only achieved by the macrophages after an interval of at least 3 days in culture after ingestion of group a cell walls. if macrophages were cultured only 24 h after ingestion of cell walls, a cytostati ...1977409679
the interactions between rifampicin and trimethoprim: an in vitro study. 1977409709
posterior mitral valve vegetation. 1977409887
antimicrobial lipids: natural and synthetic fatty acids and monoglycerides.over 40 natural or synthetic lipophilic compounds were screened for antimicrobial activity. gram (+) bacteria and yeasts but not gram (-) bacteria were affected by these agents. epimino and selena fatty acids are more active than their corresponding straight chain unsubstituted fatty acids. the position of selenium influenced the antimicrobial activity of the fatty acids. the presence and position of a double or triple bond, usually an important factor in long chain fatty acids (greater than c14 ...1977409896
[a new type of ointment base: investigations on its antimicrobial properties (author's transl)].a new ointment base (pen 32) consisting of polyalcohols prevents the growth of bacteria and fungi in concentrations above 25% (mg/mg). in a concentrated form the ointment base exhibited excellent bactericidal and fungicidal effects in vitro. this property enables the ointment base to be employed without preservatives.1977410167
[selective permeability of bacterial membranes for monovalent thallium ions].transport of monovalent thallium ions in bacterial cells was studied. an energy-dependent transport of t1+ against electrochemical gradient into the cells of s. faecalis and micrococcus lysodeikticus, according to the michaelis-menten kinetics was observed. t1+, being a k+ analog, is involved into active k+ transport. unlike k+, t1+ readily penetrates bacterial membranes, reaching the level of stationary distribution between the cells and the medium. this permits to use t1+ as a penetrating cati ...1977410460
[behavior of some microorganisms cultivated in the presence of irradiated maize starch].the bactericidal effect of water soluble radiolysis products of maize starch on escherichia coli cultures declines when the initial population grows. this toxicity varies with the culture environment and the bacterial species: bacillus subtilis, lactobacillus plantarum, streptococcus faecalis, enterobacter sp. moreover, for the eucaryote cell saccharomyces cerevisiae, wild strain or muting "small colonies", only the generating time is altered by the active parts of radiolysis products. the resul ...1977410543
non-clostridial gas gangrene in the diabetic lower limb.three cases of non-clostridial gas gangrene in diabetic lower limbs are reported. this rare condition occurs only in diabetes with peripheral neuropathy and is invariably fatal unless treated surgically. the clinical picture is deceptive with a slow, but insidiously progressive course, and the late appearance of skin necrosis. antibacterial therapy is ineffective in preventing death. a serious delay may be fostered by inconclusive bacteriological investigations. urgent radical amputation is requ ...1977410694
isolation of streptococcus equinus from non-respiratory sources in children.streptococcus equinus, a non-enterococcal streptococcus, was isolated with increased frequency in a pediatric population.1977410829
virulence of enterococci in experimental pyelonephritis. 1977411205
reconventionalization following antibiotic decontamination in man and animals. 1977411365
[studies of transmission of erythromycin and chlortetracycline resistance in group d streptococci]. 1977412018
[plasmid-conferred chlortetracyline and erythromycin resistance in group d streptococci in vitro]. 1977412019
[development of a new testmethod for surface disinfection procedures. iii. the impression method: influence of the test surface material and the types of microorganisms on the recovery rate (author's transl)].the preceding test results appear to favour the impression method used for the testing of surface disinfection procedures. further investigations were concerned with the selection of a suitable germ carrier. the high recovery rate of the bacteria and the invariably uniform quality of the germ carrier are the decisive criteria. since a dependence on the type of germ was also to be expected, we checked staph. aureus, str. faecalis, e. coli, kl. pneumoniae and p. aeruginosa for their behaviour on ...1977412353
[development of a new testmethod for surface disinfection procedures. iv. natural bacterial dying rates and their modification by environmental influences (author's transl)]. 1977412354
[development of a new testmethod for surface disinfection procedures. v. the reduction of germs by disinfection (author's transl)]. 1977412355
a humoral cytotoxic substance produced by a human killer cell line.the production of a cytotoxic factor synthesized by human haemic killer cells growing in vitro is described. the factor can be found extra- and intra-cellularly. it is released from the cells by an apocrine form of secretion, illustrated by light and electron micrographs. the culture fluid from 14c-labelled killer cells reveals numerous radioactive bands following sds-gel electrophoresis. the killing factor is precipitated by 30 to 60% saturation of ammonium sulphate. cultures of human rhabdomyo ...1977412508
[bacteria and resistance patterns in bacteriuria of hospitalized patients (author's transl)].during different periods in 1976 and 1977 the bacteria of hospitalized patients were selected at random in significant bacteriuria. the species determination was carried out exactly and the susceptibility to beta-lactam-and aminoglycoside antibiotics was investigated using the quantitative tube dilution test. the bacterial spectrum of 941 isolated strains is shown in table i. e. coli was found in 50% only. the spectrum of bacteria is different in the various hospitals, caused by regional phenome ...1977412776
a comparative investigation of the bactericidal and fungicidal effects of three phenolic disinfectants. 1977412825
the antibacterial activity of a new chloroxylenol preparation containing ethylenediamine tetraacetic acid. 1977412828
structural arrangement of polymers within the wall of streptococcus faecalis.the structure of the cell wall of streptococcus faecalis was studied in thin sections and freeze fractures of whole cells and partially purified wall fractions. also, the structures of wall preparations treated with hot trichloroacetic acid to remove non-peptidoglycan wall polymers were compared with wall preparations that possess a full complement of accessory polymers. the appearance of the wall varied with the degree of hydration of preparations and physical removal of the cell membrane from ...1978412837
[isolation and cold resistance of enterococci in ice cream].studies were a total of 85 samples of ice-cream assortments. it was found that the presence of enterococci was reduced--up to 21 per cent of the samples only had from 101 to 2000 microbial cells per cu. cm of the ice-cream mass. enterococci of the fecal group predominated--81 per cent. the regime of thermal treatment applied to the ice-cream melange produced bactericidal effect on the enterococcus microflora. after pasteurization, however, the product became contaminated with a secondary enteroc ...1977413250
[development of resistance. problems of chemotherapy in urology]. 1977413268
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