PMID(sorted ascending)
the enterococcus faecalis virulence factor elra interacts with the human four-and-a-half lim domains protein 2.the commensal bacterium enterococcus faecalis is a common cause of nosocomial infections worldwide. the increasing prevalence of multi-antibiotic resistant e. faecalis strains reinforces this public health concern. despite numerous studies highlighting several pathology-related genetic traits, the molecular mechanisms of e. faecalis virulence remain poorly understood. in this work, we studied 23 bacterial proteins that could be considered as virulence factors or involved in the enterococcus inte ...201728676674
incidence, clinical characteristics, and outcomes of nosocomial enterococcus spp. bloodstream infections in a tertiary-care hospital in beijing, china: a four-year retrospective study.enterococcus spp. are the common cause of nosocomial bloodstream infections (bsis) with high morbidity and mortality. the purpose of this study was to characterize the incidence, clinical and microbiological features, and mortality of nosocomial enterococcal bsis at a large chinese tertiary-care hospital in beijing, china.201728680588
efficacy and safety of probiotic-supplemented triple therapy for eradication of helicobacter pylori in children: a systematic review and network meta-analysis.the aim of this study was to identify the best probiotic supplementation in triple therapy for pediatric population with helicobacter pylori infection.201728681177
thymus vulgaris l. extract has antimicrobial and anti-inflammatory effects in the absence of cytotoxicity and genotoxicity.this study evaluated the biological effects of the t. vulgaris l. extract., such as antimicrobial activity on planktonic cultures and mono- and polymicrobial biofilms, cytotoxicity, anti-inflammatory activity and genotoxicity.201728683409
role of purified β-1, 3 glucan binding protein (β-gbp) from paratelphusa hydrodromus and their anti-inflammatory, antioxidant and antibiofilm properties.β- 1, 3-glucan binding protein (β-gbp), a pattern recognition protein (prp), plays a critical role in triggering the innate immune response by detecting β-glucan found on the surface of microbes. in the present study, β-gbp was purified from the haemolymph of rice field crab paratelphusa hydrodromus by affinity column chromatography. the monomeric protein ph-β-gbp appeared as a single band with a molecular weight of approximately 95 kda in sds-page analysis and its purity was determined to be 89 ...201728684323
draft genome sequence of enterococcus faecalis atcc baa-2128.while a part of the native gut microflora, the gram-positive bacterium enterococcus faecalis can lead to serious infections elsewhere in the body. the draft genome of e. faecalis strain atcc baa-2128, isolated from piglet feces, was examined. this draft genome consists of 42 contigs, 12 of which exhibit homology to annotated plasmids.201728684576
restructuring of enterococcus faecalis biofilm architecture in response to antibiotic-induced stress.bacterial biofilms are intrinsically resistant to antimicrobial treatment, which contributes to microbial persistence in clinical infections. enterococcus faecalis is an opportunistic pathogen that readily forms biofilms and is the most prevalent enterococcal species identified in healthcare-associated infections. since intrinsic resistance to multiple antibiotics is common for enterococci, and antibiotic resistance is elevated in biofilm populations, it is imperative to understand the mechanism ...201728685097
investigation and analysis of the characteristics and drug sensitivity of bacteria in skin ulcer ulcer is a common type of disease affecting patients' health and quality of life, and bacterial infection increases the difficulty of its management.201728689800
use of bioinformatics and pcr in the search for abc transporter homology among various bacteria.bioinformatics databases and search tools are utilised to produce polymerase chain reaction (pcr) primers for the amplification of an abc transporter gene from the clinically important anaerobe finegoldia magna. on sequencing, a 450 base pair amplicon showed homology with the amino acid transporter of enterococcus faecalis. little sequence data is available for f. magna and the newly isolated dna could be a useful tool in the identification of this organism in clinical specimens.200428696898
honeybee (apis mellifera)-associated bacterial community affected by american foulbrood: detection of paenibacillus larvae via microbiome analysis.honeybee (apis mellifera l.) workers act as passive vectors of paenibacillus larvae spores, which cause the quarantine disease american foulbrood (afb). we assessed the relative proportions of p. larvae within the honeybee microbiome using metabarcoding analysis of the 16 s rrna gene. the microbiome was analyzed in workers outside of the afb zone (control - afb0), in workers from asymptomatic colonies in an afb apiary (afb1), and in workers from colonies exhibiting clinical afb symptoms (afb2). ...201728698604
antimicrobial susceptibility among gram-positive and gram-negative organisms collected from the latin american region between 2004 and 2015 as part of the tigecycline evaluation and surveillance trial.the in vitro activity of tigecycline and comparator agents was evaluated against gram-positive and gram-negative isolates collected in latin american centers between 2004 and 2015 as part of the tigecycline evaluation and surveillance trial (t.e.s.t.) global surveillance study.201728701170
characterization and application of antilisterial enterocins on model fresh cheese.enterococcus faecalis strains isolated from an artisanal cheese were selected based on enterocin production against listeria monocytogenes. the strains formed biofilms and presented high hydrophobic character and good autoaggregation and coaggregation capacity with l. monocytogenes. strains l3a21m3 and l3b1k3 presented high survival under gastrointestinal conditions, were able to adhere to human intestinal cells (caco-2 and ht-29), and blocked the adhesion and invasion of l. monocytogenes. the a ...201728703625
postoperative enterococcus faecalis endophthalmitis: virulence factors leading to poor visual outcome.purpose postoperative endophthalmitis caused by enterococcus faecalis often results in poor visual outcome due to retinal destruction. we evaluated the virulence factors of enterococcus faecalis clinical isolates and analyzed their relationship with retinal destruction and poor visual outcomes in postoperative bacterial endophthalmitis (pbe) following cataract surgery.201728707018
evaluation of free or anchored antimicrobial peptides as candidates for the prevention of orthopaedic device-related infections.the prevention of implant-associated infection, one the most feared complications in orthopaedic surgery, remains a major clinical challenge and urges development of effective methods to prevent bacterial colonization of implanted devices. alpha-helical antimicrobial peptides (amps) may be promising candidates in this respect due to their potent and broad-spectrum antimicrobial activity, their low tendency to elicit resistance and possible retention of efficacy in the immobilized state. the aim ...201728707817
virulence and antibiotic resistance of enterococci isolated from healthy breastfed infants.pathogenic ability has been extensively studied in clinical enterococci, but to a lesser extent in community-derived ones. most studies to date in enterococci from healthy infants have been focused on enterococcus faecalis, despite the growing concern about nosocomial infections caused by e. faecium. in this work, we studied the antibiotic resistance and virulence determinants of 26 e. faecalis and 15 e. faecium intestinal isolates from spanish healthy breastfed infants. overall, commensal enter ...201728708453
short-term alcohol abstinence improves antibacterial defenses of chronic alcohol-consuming mice against gut bacteria-associated sepsis caused by enterococcus faecalis oral infection.the effects of short-term alcohol abstinence on host antibacterial resistance against enterococcus faecalis oral infection was investigated in chronic alcohol-consuming mice [mice with 0.1 g/day of 20% ethanol consumption for 12 or 16 weeks (cac-mice)]. these mice were highly susceptible to the infection; however, after 7 days of alcohol abstinence (aacac-mice), their antibacterial resistances were completely restored to the normal mouse level. normal mice inoculated with cac-mouse hepatic macro ...201728708971
genetic characterisation confirms sporadic occurrence of vancomycin-resistant enterococcus faecalis in copenhagen, denmark. 201728709988
antimicrobial and immunomodulatory activity of host defense peptides, clavanins and ll-37, in vitro: an endodontic perspective.endodontic treatment is mainly based on root canal disinfection and its failure may be motivated by microbial resistance. endodontic therapy can be benefitted by host defense peptides (hdps), which are multifunctional molecules that act against persistent infection and inflammation. this study aimed to evaluate the antimicrobial, cytotoxic and immunomodulatory activity of several hdps, namely clavanin a, clavanin a modified (mo) and ll-37, compared to intracanal medication ca(oh)2. hdps and ca(o ...201728712894
detection of eight foodborne bacterial pathogens by oligonucleotide array hybridization.simultaneous and rapid detection of multiple foodborne bacterial pathogens is important for the prevention of foodborne illnesses.201728713514
screening of oxalate degrading lactic acid bacteria of food origin.a screening for oxalate degrading abilities was initially carried on within lactic acid bacteria cultures of different food origin. seventy-nine strains were drop-inoculated onto mrs agar plates containing calcium oxalate. by comparing colonies diameters, 31 strains were used to inoculate, in parallel, mrs and mrs modified by sodium oxalate addition. differences in the strains' growth were assessed by colony forming unit counts. for two strains, the growth in oxalate enriched medium was signific ...201728713789
virulence genes in biofilm producer enterococcus faecalis isolates from root canal infections.enterococcus faecalis is occurring in opportunistic infections involving the oral cavity. this study aimed to evaluate the presence of e. faecalis virulence genes in dental root canal isolates recovered from advanced chronic periodontitis patients. one hundred e. faecalis isolated from dental root canal during july 2015 to oct 2016. after analysis of biofilm formation by the semi-quantitative determination in 96-well flat bottom polystyrene plates, the presence of asa, esp, efaa, ace, ebpr, gel ...201728719346
biofilm formation in enterococci: genotype-phenotype correlations and inhibition by vancomycin.enterococci are nosocomial pathogens that can form biofilms, which contribute to their virulence and antibiotic resistance. although many genes involved in biofilm formation have been defined, their distribution among enterococci has not been comprehensively studied on a genome scale, and their diagnostic ability to predict biofilm phenotypes is not fully established. here, we assessed the biofilm-forming ability of 90 enterococcal clinical isolates. major patterns of virulence gene distribution ...201728720810
isolation and characterization of lactic acid bacteria from traditional pickles of himachal pradesh, india.a total of 15 isolates of lactic acid bacteria (lab) were isolated from traditional pickles of rural and urban areas of himachal pradesh. these isolates were identified as enterococcus faecalis (7), lactobacillus plantarum (3), pediococcus pentosaceus (2), leuconostoc mesenteroides (1), lactococcus lactis (1) and enterococcus sp. (1) on the basis of morphological, biochemical and 16s rdna sequence analysis. all the labs produced exopolysaccharides, have antibiotic susceptibility, and possess act ...201728720951
antibacterial effect of azadirachta indica (neem) or curcuma longa (turmeric) against enterococcus faecalis compared with that of 5% sodium hypochlorite or 2% chlorhexidine in vitro.the purpose of this study was to compare the antibacterial properties of azadirachta indica (neem) or curcuma longa (turmeric) against enterococcus faecalis with those of 5% sodium hypochlorite or 2% chlorhexidine as root canal irrigants in vitro. the activity of neem, chlorhexidine, sodium hypochlorite, or turmeric against e. faecalis was measured on agar plates using the agar diffusion method. the tube dilution method was used to determine the minimum inhibitory concentration (mic) and minimum ...201728724858
chemical constituents of helichrysum italicum (roth) g. don essential oil and their antimicrobial activity against gram-positive and gram-negative bacteria, filamentous fungi and candida albicans.the aerial parts of helichrysum italicum (roth) g. don were subjected to hydrodistillation to obtain essential oils which had been analyzed by gas chromatography and gas chromatography coupled with mass spectrometry and tested for antimicrobial activity against 12 bacteria, two yeasts and four fungi by agar diffusion method. the essential oil yielded 0.44% (v/w) and 67 compounds accounting for 99.24% of the oil were identified with a high content of oxygenated sesquiterpenes (61.42%). the most o ...201728725151
integrating highly quantitative proteomics and genome-scale metabolic modeling to study ph adaptation in the human pathogen enterococcus faecalis.genome-scale metabolic models represent the entirety of metabolic reactions of an organism based on the annotation of the respective genome. these models commonly allow all reactions to proceed concurrently, disregarding the fact that at no point all proteins will be present in a cell. the metabolic reaction space can be constrained to a more physiological state using experimentally obtained information on enzyme abundances. however, high-quality, genome-wide protein measurements have been chall ...201628725473
soymilk fermentation by enterococcus faecalis vb43 leads to reduction in the immunoreactivity of allergenic proteins β-conglycinin (7s) and glycinin (11s).food allergies represent a serious problem affecting human health and soy proteins rank among the most allergenic proteins from food origin. the proteolytic enzymes produced by lactic acid bacteria (lab) can hydrolyse the major allergens present in soybean, reducing their immunoreactivity. many studies have reported the ability of lab to ferment soy-based products; while the majority of them focus on the improvement of the sensory characteristics and functionality of soy proteins, a lack of info ...201728726509
in vitro activity of telavancin against clinically important gram-positive pathogens from 69 u.s. medical centers (2015): potency analysis by u.s. census divisions.a total of 8,072 gram-positive isolates collected from 69 medical centers among all 9 u.s. census divisions during the 2015 sentry antimicrobial surveillance program were included. telavancin had minimal inhibitory concentration (mic)50 and mic90 values of 0.03/0.06 μg/ml, respectively, against methicillin-susceptible (mssa) and methicillin-resistant staphylococcus aureus (mrsa). similar activity was also observed among coagulase-negative staphylococci (mic50/90, 0.03/0.06 μg/ml; 100.0% inhibite ...201728727951
hypochlorite solution for root canal irrigation that lacks a chlorinated odor.this is an in vitro study to develop a formulation of a hypochlorite solution for root canal irrigation that lacks a chlorinated odor. the antibacterial effect, tissue dissolution efficacy, and the cytotoxicity of the solution were assessed in cell culture and were compared with those of commercial sodium hypochlorite (naocl) solutions.201728729797
in vitro stepwise selection of reduced susceptibility to lipoglycopeptides in enterococci.the propensity of oritavancin to select for stably elevated oritavancin minimum inhibitory concentrations (mics) was studied by serial passaging of strains in broth containing oritavancin for 20days. seven clinical strains of enterococcus faecalis and e. faecium were studied; they included vancomycin-susceptible and both vana and vanb vancomycin-resistant isolates. stepwise oritavancin selection yielded stably elevated oritavancin mics in six of the seven strains, with mic increases ranging from ...201728733126
seven new lignan glycosides from the branches of alangium kurzii craib var. new lignan glycosides (1-3, 8-10, and 14) and 17 known compounds were isolated from the branches of alangium kurzii craib var. laxifolium. their structures were established by spectroscopic analysis and circular dichroism (cd) and x-ray analysis. the isolated compounds were evaluated for their antibacterial activities against staphylococcus aureus ci1011, streptococcus suis ci1608, salmonella gallinarum ci0912, enterococcus faecalis ci1304, aeromonas hydrophila ci1008, escherichia coli ci1 ...201728733150
[bifidobacterium scardovii isolated from 2 consecutive urine samples].bifidobacterium scardovii species consists of facultative anaerobic gram-positive rods whose growth is stimulated by co2 and anaerobiosis. exceptionally it has been associated with infections in humans. an elderly male patient with a urinary tract infection due to b. scardovii and enterococcus faecalis is presented here; both microorganisms were isolated from two consecutive urine samples. the bacillus did not grow on standard media, but on chocolate agar incubated in co2 and on supplemented bru ...201728734711
telavancin activity in vitro tested against a worldwide collection of gram-positive clinical isolates (2014).telavancin activity was measured against gram-positive pathogens collected worldwide during 2014 using the revised broth microdilution testing method. the results were compared with previous reports.201728735051
activity of dalbavancin tested against gram-positive clinical isolates causing skin and skin structure infections in paediatric patients from us hospitals (2014 - 2015).this study provides an in vitro analysis of dalbavancin activity against isolates causing skin and skin structure infections (sssis) in children.201728735053
safety evaluation of enterocin producer enterococcus sp. strains isolated from traditional turkish cheeses.the purpose of this study was to determine the antimicrobial activity and occurrence of bacteriocin structural genes in enterococcus spp. isolated from different cheeses and also investigate some of their virulence factors. enterococcus strains were isolated from 33 different cheeses. enterococcus faecium (6 strains) and enterococcus faecalis (5 strains) enterocin-producing strains were identified by 16s rdna analyses. structural genes enta, entb, entp and entx were detected in some isolates. mu ...201728735309
the usefulness of biosynthetic vascular graft omniflow ii and autologous veins for the treatment of massive infection of dacron vascular graft with enterococcus faecalis hlar.infections of vascular grafts are the most severe complications in vascular surgery. we present the case of a 73-year-old male with infection of a dacron prosthesis with a strain of enterococcus faecalis. the patient was treated with replacement of a full prosthesis with a combined graft constructed from biosynthetic vascular graft omniflow and autologous veins. this graft is recommended for implantation in patients with a higher risk of infection. our case is one of the first reported usage of ...201728735332
genetic diversity and antimicrobial resistance profile of enterococcus faecalis isolated from broilers with vertebral osteomyelitis in southeast brazil.vertebral osteomyelitis (vo) is a worldwide emerging disease that affects broilers. recently, the isolation of enterococcus faecalis in cases of the disease has been described. this study aimed at determining the genetic diversity and antimicrobial resistance profile of 12 enterococcus faecalis strains isolated from broilers with vo. strains were isolated from nine flocks from six farms in a high-density poultry production area in southeast brazil and were evaluated using multilocus sequence typ ...201728737063
characterization of enterococcus faecalis isolates by chicken embryo lethality assay and eric-pcr.enterococcus faecalis is the major causative agent of amyloid arthropathy in chickens. given the difficulty of estimating the risk from field strains, the embryo lethality assay (ela) is proposed in this study as a model to predict the virulence of 68 avian e. faecalis strains. additionally, enterobacterial repetitive intergenic consensus polymerase chain reaction (eric-pcr) was used to characterize the genetic diversity of the e. faecalis strains. the ela was performed 10 times with subsets of ...201728737107
synthesis and antimicrobial activity of chiral quaternary n-spiro ammonium bromides with 3',4'-dihydro-1'h-spiro[isoindoline-2,2'-isoquinoline] skeleton.a new class of highly functionalized tetrahydroisoquinolines with a quaternary carbon stereocenter was synthesized starting from an easily accessible l-tartaric acid. nine strains of bacteria (staphylococcus aureus, streptococcus pyogenes, streptococcus mutans, streptococcus salivarius, bacillus subtilis, enterococcus faecalis, moraxella catarrhalis, escherichia coli, campylobacter jejuni) were used for the determination of minimal inhibitory concentration (mic) and minimal bactericidal concentr ...201728740363
bacterial size matters: multiple mechanisms controlling septum cleavage and diplococcus formation are critical for the virulence of the opportunistic pathogen enterococcus faecalis.enterococcus faecalis is an opportunistic pathogen frequently isolated in clinical settings. this organism is intrinsically resistant to several clinically relevant antibiotics and can transfer resistance to other pathogens. although e. faecalis has emerged as a major nosocomial pathogen, the mechanisms underlying the virulence of this organism remain elusive. we studied the regulation of daughter cell separation during growth and explored the impact of this process on pathogenesis. we demonstra ...201728742152
determination of disk diffusion and mic quality control ranges for nafithromycin (wck 4873), a new lactone-ketolide.disk diffusion and mic quality control (qc) ranges were determined for nafithromycin, a new lactone-ketolide, following completion of a nine-laboratory, clinical and laboratory standards institute (clsi) m23-defined tier 2 study. five qc strains consistent with the spectrum of activity of nafithromycin were tested: staphylococcus aureus atcc 25923 (disk only), s. aureus atcc 29213 (broth only), enterococcus faecalis atcc 29212 (broth only), streptococcus pneumoniae atcc 49619 (disk and broth), a ...201728747372
effect of different mixing methods on the bacterial microleakage of white portland cement and white mineral trioxide aggregate.background. of this study was to investigate the effect of different mixing methods (ultrasonic, amalgamator, and conventional) on the bacterial microleakage of white portland cement (wpc) and white mta (tooth-colored formula, dentsply, tulsa dental, tulsa, ok). methods. a hundred human single-rooted permanent teeth were decoronated to obtain 14 mm of root length in all the samples. the root canals were cleaned, shaped and obturated. three millimeters of each root apex were cut off and randomly ...201728748048
assessment of facultative anaerobes from the root canals of deciduous molars: an in vivo study.background. the current research aimed to assess the prevalence of facultative anaerobes isolated from the root canals of deciduous molars. methods. the present research enrolled 60 children in the 6‒9-year age group based on clinical and radiographic findings. under aseptic conditions, access cavities were prepared followed by collection of samples from infected root canals with the help of sterile paper points. the samples thus obtained were subjected for microbial assay. results. it was found ...201728748050
buwchitin: a ruminal peptide with antimicrobial potential against enterococcus faecalis.antimicrobial peptides (amps) are gaining popularity as alternatives for treatment of bacterial infections and recent advances in omics technologies provide new platforms for amp discovery. we sought to determine the antibacterial activity of a novel antimicrobial peptide, buwchitin, against enterococcus faecalis. buwchitin was identified from a rumen bacterial metagenome library, cloned, expressed and purified. the antimicrobial activity of the recombinant peptide was assessed using a broth mic ...201728748180
photodynamic therapy with high-power led mediated by erythrosine eliminates enterococcus faecalis in planktonic forms.the failure of endodontic treatment is linked to the presence of microorganisms, particularly enterococcus faecalis, in the root canals. this study evaluated the effectiveness of photodynamic therapy (pdt) using erythrosine irradiated by a high-power curing light on a planktonic suspension culture of e. faecalis.201728750753
draft genome sequence of enterococcus faecalis dd14, a bacteriocinogenic lactic acid bacterium with anti-clostridium activity.we report the draft genome sequence of enterococcus faecalis dd14, a strain isolated from meconium of a healthy newborn at roubaix hospital (france). the strain displayed antagonism against a set of gram-positive bacteria through concomitant production of lactic acid and bacteriocin. the genome has a size of 2,893,365 bp and a 37.3% g+c ratio and is predicted to contain at least 2,755 coding sequences and 62 rnas.201728751398
do quaternary ammonium monomers induce drug resistance in cariogenic, endodontic and periodontal bacterial species?antibacterial monomers were developed to combat oral biofilm acids and caries; however, little is known on whether quaternary ammonium monomers (qams) would induce drug resistance in oral bacteria. the objective of this study was to investigate the effects of new antimicrobial monomers dimethylaminohexadecyl methacrylate (dmahdm) and dimethylaminododecyl methacrylate (dmaddm) on the induction of drug resistance in eight species of cariogenic, endodontic and periodontal bacteria for the first tim ...201728755761
cyclohexene-fused 1,3-oxazines with selective antibacterial and antiparasitic action and low cytotoxic effects.oxazine derivatives, a class of heterocyclic compounds, exhibit a variety of biological properties, such as anticonvulsant and antitumor activities. in this study, we evaluated the effect of two cyclohexene-fused 1,3-oxazines (cis‑1-benzyl-n-phenyl-1,4,4a,5,8,8a-hexahydro-3,1-benzoxazin-2-imine (1) and trans‑n-phenyl-1,4,4a,5,8,8a-hexahydro-3,1-benzoxazin-2-imine (2)) in cultures of bacillus cereus, enterococcus faecalis, escherichia coli, klebsiella pneumoniae, salmonella enterica, serratia mar ...201728755871
efficacy of photon-induced photoacoustic streaming in the reduction of enterococcus faecalis within the root canal: different settings and different sodium hypochlorite concentrations.the purpose of this study was to determine the effectiveness of laser-activated irrigation by photon-induced photoacoustic streaming (pips) in the reduction of enterococcus faecalis in root canal disinfection, varying laser energy output, and sodium hypochlorite (naocl) concentration. for effective removal of the smear layer, the sequence and resting time of the final irrigation steps were modified compared with the standard pips protocol.201728756961
in vitro synergistic activities of cefazolin and nisin a against mastitis pathogens.first-generation cephalosporins such as cefazolin (cez) have been widely used for mastitis treatment in dairy cattle. however, the use of antibiotics results in the presence of antibiotic residues in milk, which is used for human consumption. nisin a, a bacteriocin produced by lactococcus lactis, has been used as a broad-spectrum food preservative for over 50 years. therefore, a combination of cez and nisin a might provide an extended activity spectrum against mastitis pathogens and reduce the a ...201728757508
first case of multidrug-resistant enterococcus faecalis isolated from herding horse in xinjiang, western china. 201728760414
the potential of alcohol release doorplates to reduce surface contamination during hand contact.optimal hand hygiene may be compromised by contact with contaminated environmental surfaces.201728760635
additive effect of photoactivated disinfection on the antibacterial activity of qmix 2in1 against 6-week enterococcus faecalis biofilms: an in vitro study.evaluation of the additive effect of photodynamic therapy (pdt) on the antibacterial activity of 2.5% sodium hypochlorite (naocl) and qmix against 6-week enterococcus faecalis biofilms contaminated root canals.201728761252
a prospective randomised trial of isolated pathogens of surgical site infections (ssi).every surgical wound is colonized by bacteria, but only a small percentage displays symptoms of infection. the distribution of pathogens isolated in surgical site infections has not significantly changed over the last decades. staph. aureus, coag(-) staphylococci, enterococcus spp and e. coli are the main strains appearing. in addition, a continuously rising proportion of surgical site infections caused by resistant bacterial species (mrsa, c. albicans) has been reported.201728761643
antimicrobial activities of widely consumed herbal teas, alone or in combination with antibiotics: an in vitro study.because of increasing antibiotic resistance, herbal teas are the most popular natural alternatives for the treatment of infectious diseases, and are currently gaining more importance. we examined the antimicrobial activities of 31 herbal teas both alone and in combination with antibiotics or antifungals against some standard and clinical isolates of pseudomonas aeruginosa, acinetobacter baumannii, escherichia coli, klebsiella pneumoniae, enterococcus faecalis, methicillin susceptible/resistant s ...201728761777
vancomycin-resistant enterococci with vana gene in treated municipal wastewater and their association with human hospital strains.vancomycin-resistant enterococci (vre) are pathogens of increasing medical importance. in brno, czech republic, we collected 37 samples from the effluent of a wastewater treatment plant (wwtp), 21 surface swabs from hospital settings, and 59 fecal samples from hospitalized patients and staff. moreover, we collected 284 gull cloacal swabs from the colony situated 35km downstream the wwtp. samples were cultured selectively. enterococci were identified using maldi-tof ms, phenotypically tested for ...201728763660
revisiting nitrofurantoin for vancomycin resistant enterococci.enterococcal infection has emerged as a major therapeutic challenge. emergence of high level aminoglycoside resistance (hlar) and vancomycin-resistant enterococcus (vre) has further limited the drug therapy in enterococcal infections. however, nitrofuratoin being an old drug reported to have less resistance in comparison to the other classes of antimicrobial agents.201728764160
fabrication and appraisal of poly (lactic-co-glycolic acid) - moxifloxacin nanoparticles using vitamin e-tpgs: a potential intracanal drug delivery agent.nanotechnology is gaining momentum in the search for ideal biomaterials by dental researchers. the expansible nature of endodontology deems exploration, learning and scrutinizing newer avenues which have the potential to be applied and investigated. the popularity of polymers as drug delivery vehicles has opened avenues for their application in the root canal system. this study utilized application of biodegradable polymers as potential drug delivery vehicles against enterococcus faecalis, one o ...201728764283
microbial load in septic and aseptic procedure rooms.highly effective measures to prevent surgical wound infections have been established over the last two decades. we studied whether the strict separation of septic and aseptic procedure rooms is still necessary.201728764834
septic episodes in a premature infant after in utero exposure to rituximab.rituximab is an increasingly used immunotherapeutic agent for women of reproductive age for treatment of autoimmune diseases, leukemias, and lymphomas. rituximab is a chimeric monoclonal antibody that targets b-cell surface antigen cd20 and can cross the placenta. current evidence of the impact of this medication on the developing fetus is limited, but there is little to suggest that fetal exposure to this medication places an infant at increased risk of immunosuppression and subsequent infectio ...201728768852
eco-friendly fabrication of ag nanostructures using the seed extract of pedalium murex, an ancient indian medicinal plant: histopathological effects on the zika virus vector aedes aegypti and inhibition of biofilm-forming pathogenic bacteria.the control of zika virus mosquito vectors and well as the development of drugs in the fight against biofilm-forming microbial pathogens, are timely and important challenges in current bionanoscience. here we focused on the eco-friendly fabrication of ag nanostructures using the seed extract of pedalium murex, an ancient indian medicinal plant. initial confirmation of ag nanoparticles (agnps) production was showed by a color change from transparent to dark brown. the uv-visible spectrum (476nm), ...201728772238
synthesis and characterization of new chlorhexidine-containing nanoparticles for root canal disinfection.root canal system disinfection is limited due to anatomical complexities. better delivery systems of antimicrobial agents are needed to ensure efficient bacteria eradication. the purpose of this study was to design chlorhexidine-containing nanoparticles that could steadily release the drug. the drug chlorhexidine was encapsulated in poly(ethylene glycol)-block-poly(l-lactide) (peg-b-pla) to synthesize bilayer nanoparticles. the encapsulation efficiency was determined through thermogravimetric an ...201628773576
conjugal transfer of aac(6')ie-aph(2″)ia gene from native species and mechanism of regulation and cross resistance in enterococcus faecalis mcc3063 by real time-pcr.high level aminoglycoside resistance (hlar) in the lactic acid bacteria (lab) derived from food animals is detrimental. the aim of this study was to investigate the localization and conjugal transfer of aminoglycoside resistance genes, aac(6')ie-aph(2″)ia and aph(3')iiia in different enterococcus species. the cross resistance patterns in enterococcus faecalis mcc3063 to clinically important aminoglycosides by real time pcr were also studied. southern hybridization experiments revealed the presen ...201728774859
antimicrobial efficacy of triple antibiotic-eluting polymer nanofibers against multispecies biofilm.the elimination of microbial flora in cases of immature permanent teeth with necrotic pulp is both key and a challenging goal for the long-term success of regenerative therapy. recent research has focused on the development of cell-friendly intracanal drug delivery systems. this in vitro study aimed to investigate the antimicrobial action of 3-dimensional (3d) tubular-shaped triple antibiotic-eluting nanofibrous constructs against a multispecies biofilm on human dentin. polydioxanone polymer sol ...201728778504
antibacterial and antifungal activity of endodontic intracanal medications.the sterilization of the entire root canal system represents the main goal of every endodontist, given the fact that the control of the microbial flora is the key point of every root canal treatment. the diversity of microorganisms found inside the root canal and also the resistance of some bacterial species to intracanal medications led to a continuous development of new endodontic products. the present study focuses on the comparison of the antibacterial and antifungal properties of different ...201728781531
coupling metagenomics with cultivation to select host-specific probiotic microorganisms for subtropical demonstrate a non-empirical workflow to select host-specific probiotics for aquaculture industry.201728783248
the individual contribution of starter and non-starter lactic acid bacteria to the volatile organic compound composition of caciocavallo palermitano cheese.the contribution of two starter (lactobacillus delbrueckii and streptococcus thermophilus) and nine non-starter (enterococcus casselliflavus, enterococcus faecalis, enterococcus durans, enterococcus gallinarum, lactobacillus casei, lactobacillus paracasei, lactobacillus rhamnosus, pediococcus acidilactici and pediococcus pentosaceus) species of lactic acid bacteria (lab) to the volatile organic compounds (vocs) of caciocavallo palermitano cheese was investigated. the strains used in this study w ...201728783535
characterization of the tertiary structure of the peptidoglycan of enterococcus faecalis.solid-state nmr spectra of whole cells and isolated cell walls of enterococcus faecalis grown in media containing combinations of (13)c and (15)n specific labels in d- and l-alanine and l-lysine (in the presence of an alanine racemase inhibitor alaphosphin) have been used to determine the composition and architecture of the cell-wall peptidoglycan. the compositional variables include the concentrations of (i) peptidoglycan stems without bridges, (ii) d-alanylated wall teichoic acid, (iii) cross- ...201728784459
initial bacterial adhesion on different yttria-stabilized tetragonal zirconia implant surfaces in vitro.bacterial adhesion to implant biomaterials constitutes a virulence factor leading to biofilm formation, infection and treatment failure. the aim of this study was to examine the initial bacterial adhesion on different implant materials in vitro. four implant biomaterials were incubated with enterococcus faecalis, staphylococcus aureus and candida albicans for 2 h: 3 mol % yttria-stabilized tetragonal zirconia polycrystal surface (b1a), b1a with zirconium oxide (zro₂) coating (b2a), b1a with zirc ...201328788415
anti-enterococcal activities of pentacyclic triterpenes.asiatic (aa) and ursolic (ua) acids are widely studied phytochemicals, but their antimicrobial properties are still poorly understood. therefore our research has focused on their activity against uropathogenic enterococcus faecalis strains.201728791824
media from macrophages co-incubated with enterococcus faecalis induces epithelial cell monolayer reassembly and altered cell morphology.signal exchange between intestinal epithelial cells, microbes and local immune cells is an important mechanism of intestinal homeostasis. given that intestinal macrophages are in close proximity to both the intestinal epithelium and the microbiota, their pathologic interactions may result in epithelial damage. the present study demonstrates that co-incubation of murine macrophages with e. faecalis strains producing gelatinase (gele) and serine protease (spre) leads to resultant condition media ( ...201728793333
disulfide bonds: a key modification in bacterial extracytoplasmic proteins.disulfide bonds are a common posttranslational modification that contributes to the folding and stability of extracytoplasmic proteins. almost all organisms, from eukaryotes to prokaryotes, have evolved enzymes to make and break these bonds. accurate and efficient disulfide bond formation can be vital for protein function; therefore, the enzymes that catalyze disulfide bond formation are involved in multiple biological processes. recent advances clearly show that oral bacteria also have the abil ...201728797211
methyl propiolate and 3-butynone: starting points for synthesis of amphiphilic 1,2,3-triazole peptidomimetics for antimicrobial evaluation.a library of 29 small 1,4-substituted 1,2,3-triazoles was prepared for studies of antimicrobial activity. the pharmacophore model investigated with these substrates was based on small peptidomimetics of antimicrobial peptides and antimicrobials isolated from marine organisms from sub-arctic regions. using methyl 1,2,3-triazole-carboxylates and 1,2,3-triazole methyl ketones prepared through "click" chemistry we were able to synthesize the different cationic amphiphiles through three steps or less ...201728797773
genomic insights into the pathogenicity and environmental adaptability of enterococcus hirae r17 isolated from pork offered for retail sale.genetic information about enterococcus hirae is limited, a feature that has compromised our understanding of these clinically challenging bacteria. in this study, comparative analysis was performed of e. hirae r17, a daptomycin-resistant strain isolated from pork purchased from a retail market in beijing, china, and three other enterococcal genomes (enterococcus faecium do, enterococcus faecalis v583, and e. hirae atcc(™) 9790). some 1,412 genes were identified that represented the core genome t ...201728799224
proinflammatory mg-63 cells response infection with enterococcus faecalis cps2 evaluated by the expression of tlr-2, il-1β, and inos mrna.we have previously demonstrated that unencapsulated enterococcus faecalis cps2 inhibits biofilm formation of candida albicans, a fungus commonly found with e. faecalis in periapical lesion. in this study, we compared encapsulated and unencapsulated e. faecalis cps2 strains relationship with osteoblastic (mg-63) cells, whereas e. faecalis atcc 29212 were used as a reference strain.201728800779
efficacy of radiant catalytic ionization to reduce bacterial populations in air and on different surfaces.air contamination by biological agents is often observed in medical or veterinary facilities and industrial plants. bioaerosols may sediment and pose the surface contamination. microorganisms present on them may become a source of infections among humans and food contamination. this study determined the use of oxidative gases, including ozone and peroxide, generated by the radiant catalytic ionization (rci) cell for the inactivation of acinetobacter baumannii, escherichia coli, enterococcus faec ...201728803189
growth- and stress-induced pasta kinase phosphorylation in enterococcus faecalis.transmembrane ser/thr kinases containing extracellular pasta domains are ubiquitous among actinobacteria and firmicutes. such pasta kinases regulate critical processes including antibiotic resistance, cell division, toxin production, virulence, and are essential for viability in certain organisms. based on in vitro studies with purified extra- and intracellular fragments of pasta kinases, a model for signaling has been proposed, in which the extracellular pasta domains bind as-yet-undefined liga ...201728808126
[distribution and drug resistance of pathogens at hematology department of jiangsu province from 2014 to 2015: results from a multicenter, retrospective study].objective: to describe the distribution and drug resistance of pathogens at hematology department of jiangsu province from 2014 to 2015 to provide reference for empirical anti-infection treatment. methods: pathogens were from hematology department of 26 tertiary hospitals in jiangsu province from 2014 to 2015. antimicrobial susceptibility testing was carried out according to a unified protocol using kirby-bauer method or agar dilution method. collection of drug susceptibility results and corresp ...201728810329
in vitro antiplasmodial activity and prophylactic potentials of extract and fractions of trema orientalis (linn.) stem bark.trema orientalis (t. orientalis linn) has been used in the management of malaria in the western part of nigeria and despite its application in ethnomedicine, there is dearth of scientific evidence to justify the acclaimed prophylactic antimalarial usage of the plant. the aim of this study is to assess the in vitro antiplasmodial cell-free assay and chemopreventive efficacy of the methanol extract of the stem bark of t. orientalis and its fractions as a prophylactic regimen for malaria prevention ...201728810849
in vitro activity of gentamicin-loaded bioabsorbable beads against different microorganisms.osteomyelitis is responsible for high treatment costs, long hospital stays, and results in substantial morbidity. treatment with surgical debridement and antibiotic-impregnated polymethylmetacrylate (pmma) beads is the standard of care, providing high local but low serum antibiotic concentrations, thereby avoiding systemic toxicity. however, for several reasons, the beads require surgical removal. alternative antibiotic delivery systems should improve the treatment of bone infection, actively en ...201328811436
the antibacterial and antifungal activity of six essential oils and their cyto/genotoxicity to human hel 12469 cells.six essential oils (from oregano, thyme, clove, lavender, clary sage, and arborvitae) exhibited different antibacterial and antifungal properties. antimicrobial activity was shown against pathogenic (escherichia coli, salmonella typhimurium, yersinia enterocolitica, staphylococcus aureus, listeria monocytogenes, and enterococcus faecalis) and environmental bacteria (bacillus cereus, arthrobacter protophormiae, pseudomonas fragi) and fungi (chaetomium globosum, penicillium chrysogenum, cladospori ...201728811611
antibacterial properties of nonwoven wound dressings coated with manuka honey or methylglyoxal.manuka honey (mh) is used as an antibacterial agent in bioactive wound dressings via direct impregnation onto a suitable substrate. mh provides unique antibacterial activity when compared with conventional honeys, owing partly to one of its constituents, methylglyoxal (mgo). aiming to investigate an antibiotic-free antimicrobial strategy, we studied the antibacterial activity of both mh and mgo (at equivalent mgo concentrations) when applied as a physical coating to a nonwoven fabric wound dress ...201728813014
[elimination of entercoccus faecalis with different disinfection methods in root canals in vitro].to compare the elimination effect against e.faecalis in root canals with different methods.201728815239
use of whey as a culture medium for bacillus clausii for the production of protein hydrolysates with antimicrobial and antioxidant activity.the use of whey as a natural culture medium to produce hydrolysates with antimicrobial from bacillus clausii is the objective of this research. the fermentation process was carried out at 25 ℃ for five days, where proteolytic activity, soluble peptides quantification, and antimicrobial activity using the disc diffusion method were determined every day. the fermented whey reached values ​​of proteolytic activity between 90 and 260 u ml(-1) and a production of soluble peptides between 100 and 2070 ...201728816527
in vitro selection and identification of potential probiotics isolated from the gastrointestinal tract of nile tilapia, oreochromis gut bacteria can be used as probiotics for aquaculture. the aim of this study is to screen and identify beneficial probiotic bacteria from the gut of nile tilapia, oreochromis niloticus. nine out of one hundred thirty-five isolates were non-pathogenic through intraperitoneal injection and had antibacterial activities with at least a strain from the five isolated fish pathogens, aeromonas sobria, aeromonas hydrophila, pseudomonas aeruginosa, pseudomonas putida, and staphylococcus aureus. fur ...201728819794
small alarmone synthetases as novel bacterial rna-binding proteins.the alarmone nucleotides guanosine pentaphosphate (pppgpp) and tetraphosphate (ppgpp), collectively referred to as (p)ppgpp, are key regulators of bacterial growth, stress adaptation, antibiotic tolerance and pathogenicity. we have recently shown that the small alarmone synthetase (sas) relq from the gram-positive pathogen enterococcus faecalis has an rna-binding activity (beljantseva et al. 2017). relq's activities as an enzyme and as a rna-binding protein are mutually incompatible: binding of ...201728820325
microbiology of root canal infections.inflammatory reaction in the periapical tissues is induced by microbial infection in the root canal system. the aim of root canal treatment is to preserve healthy periapical tissues or to provide healing of them in restorable teeth, which have sufficient periodontal support. the amount of microbial cells in the root canal system and their virulence as well as host responses influence on the degree of periapical inflammation and symptoms. microbial biofilm formation is typically seen on root cana ...201628826437
physicochemical characterization and antibacterial activity of rajata bhasma and silver nanoparticle.bhasma, an ayurvedic metallo-mineral preparation, is claimed to be biologically produced nanoparticles. rajata (silver) is a noble metal known for its antimicrobial activity. rajata bhasma (rb) is expected to be composed of nanoparticles. with all these facts in place, this study was conducted to evaluate rb for the presence of silver nanoparticle (snp) and its antimicrobial effect.201728827959
investigation of effect of 1,8-cineole on antimicrobial activity of chlorhexidine gluconate.chlorhexidine belongs to a group of medicines called antiseptic antibacterial agents. chlorhexidine is commonly used for the care and clean off the skin, hands, and wounds. in recent years, medicinal and aromatic plants have been used for prevention of disease, maintaining health, and improving disease in traditional and modern medicine as a medicament. according to recent research, cineole is the isolated active agent of eucalyptus oil and possesses antimicrobial activity. it was demonstrated t ...201728827963
how proteases from enterococcus faecalis contribute to its resistance to short α-helical antimicrobial peptides.hyl-20 (gilsslwkklkkiiak-nh2) is an analogue of a natural antimicrobial peptide (amp) previously isolated from the venom of wild bee. we examined its antimicrobial activity against three strains of enterococcus faecalis while focusing on its susceptibility to proteolytic degradation by two known proteases-gelatinase (gele) and serine protease (spre)-which are secreted by these bacterial strains. we found that hyl-20 was primarily deamidated at its c-terminal which made the peptide susceptible to ...201728830077
in vitro evaluation of cytotoxic, anti-proliferative, anti-oxidant, apoptotic, and anti-microbial activities of cladonia pocillum.the aim of this study was to investigate the anti-proliferative, apoptotic, cytotoxic, and anti-oxidant effects of extracts from the lichen cladonia pocillumon human breast cancer cells (mcf-7), and to characterize the anti-microbial features.  mcf-7 cells were treated with methanolic c. pocillum extract for 24h. the cytotoxicity of the extract was tested with mtt. moreover, its anti-proliferative effects were examined with immunocytochemical method. apoptosis and biochemical parameters were det ...201728838343
antimicrobial and antioxidant activities of the extract and fractions of tetradenia riparia (hochst.) codd (lamiaceae) leaves from brazil.tetradenia riparia (lamiaceae) is native to central africa popularly known as myrrh, used in folk medicine to treat various diseases like malaria, gastroenteritis, and tropical skin disease. this research was to evaluate the antioxidant and antibacterial activities of the crude extract (ce) and fractions (fr) of the t. riparia by classical chromatography. the ce of t. riparia leaves was submitted to column chromatographic fractionation to obtain four fractions of the interest, which were identif ...201728840299
molecular determinants of the matrix thickening of a dual-species pseudomonas aeruginosa and enterococcus faecalis biofilm.biofilms are microbial communities that inhabit various surfaces and are surrounded by extracellular matrices (ecms). clinical microbiologists have shown that the majority of chronic infections are caused by biofilms, after introduction of the first biofilm infection model by j.w. costerton and his colleagues (1). however, treatments for chronic biofilm infections are still limited to surgical removal of the infected sites. pseudomonas aeruginosa and enterococcus faecalis are two frequently iden ...201728842537
infectious endocarditis from enterococcus faecalis associated with tubular adenoma of the sigmoid colon.enterococcus faecalis (e. faecalis), a constituent of the gut microbiota, can be associated with both colonic lesions and endocarditis. since this microorganism is one of the endocarditis etiological agents, there is a need for greater study in regard to the association with endocarditis and colonic lesions.201728848681
discovery of phylloseptins that defense against gram-positive bacteria and inhibit the proliferation of the non-small cell lung cancer cell line, from the skin secretions of phyllomedusa frogs.the growing occurrence of bacterial resistance to conventional antibiotics has called for the development of new classes of antimicrobial agents. antimicrobial peptides (amps) with broad antimicrobial spectrum derived from frog skin secretions have been demonstrated to be promising candidates for new antibiotic development. a proven rich source of these compounds are the skin secretions of the frogs in the phyllomedusa genus. in this study, two novel phylloseptin peptides-phylloseptin-pta and ph ...201728850103
in vitro antimicrobial activity screening of xylaria hypoxylon.fungi have a potential of using both as nutritive and medicinal food stuff. because of containing several therapeutic agents, they are reported to be used for hundreds of years to treat several diseases caused by bacteria, fungi, viruses and parasites. the aim of this study is to determine the in vitro antimicrobial activity of xylaria hypoxylon, which were collected from yomra, trabzon, turkey.201628852719
enhancing antibacterial effect of sodium hypochlorite by low electric current-assisted sonic agitation.this research focused on the effects of low electric current (μe)-assisted sonic agitation of sodium hypochlorite on enterococcus faecalis infected human root dentin.201728854274
effect of conventional irrigation and photoactivated disinfection on enterococcus faecalis in root canals: an in vitro study.a study was done to evaluate the antimicrobial efficacy of sodium hypochlorite (naocl) and photoactivated disinfection (pad) on enterococcus faecalis.201728855761
diversity of antagonistic bacteria isolated from medicinal plant peganum harmala l.the antimicrobial activity of plant extract of peganum harmala, a medicinal plant has been studied already. however, knowledge about bacterial diversity associated with different parts of host plant antagonistic to different human pathogenic bacteria is limited. in this study, bacteria were isolated from root, leaf and fruit of plant. among 188 bacterial isolates isolated from different parts of the plant only 24 were found to be active against different pathogenic bacteria i.e. escherichia coli ...201728855824
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