nitric oxide charged catheters as a potential strategy for prevention of hospital acquired infections.catheter-associated hospital-acquired infections (hai's) are caused by biofilm-forming bacteria. using a novel approach, we generated anti-infective barrier on catheters by charging them with nitric oxide (no), a naturally-produced gas molecule. no is slowly released from the catheter upon contact with physiological fluids, and prevents bacterial colonization and biofilm formation onto catheter surfaces.201728410367
enterococcus faecalis bacteriocin entv inhibits hyphal morphogenesis, biofilm formation, and virulence of candida albicans.enterococcus faecalis, a gram-positive bacterium, and candida albicans, a fungus, occupy overlapping niches as ubiquitous constituents of the gastrointestinal and oral microbiome. both species also are among the most important and problematic, opportunistic nosocomial pathogens. surprisingly, these two species antagonize each other's virulence in both nematode infection and in vitro biofilm models. we report here the identification of the e. faecalis bacteriocin, entv, produced from the entv (ef ...201728396417
microbiology of root canal infections.inflammatory reaction in the periapical tissues is induced by microbial infection in the root canal system. the aim of root canal treatment is to preserve healthy periapical tissues or to provide healing of them in restorable teeth, which have sufficient periodontal support. the amount of microbial cells in the root canal system and their virulence as well as host responses influence on the degree of periapical inflammation and symptoms. microbial biofilm formation is typically seen on root cana ...201628826437
initial bacterial adhesion on different yttria-stabilized tetragonal zirconia implant surfaces in vitro.bacterial adhesion to implant biomaterials constitutes a virulence factor leading to biofilm formation, infection and treatment failure. the aim of this study was to examine the initial bacterial adhesion on different implant materials in vitro. four implant biomaterials were incubated with enterococcus faecalis, staphylococcus aureus and candida albicans for 2 h: 3 mol % yttria-stabilized tetragonal zirconia polycrystal surface (b1a), b1a with zirconium oxide (zro₂) coating (b2a), b1a with zirc ...201328788415
in vitro activity of gentamicin-loaded bioabsorbable beads against different microorganisms.osteomyelitis is responsible for high treatment costs, long hospital stays, and results in substantial morbidity. treatment with surgical debridement and antibiotic-impregnated polymethylmetacrylate (pmma) beads is the standard of care, providing high local but low serum antibiotic concentrations, thereby avoiding systemic toxicity. however, for several reasons, the beads require surgical removal. alternative antibiotic delivery systems should improve the treatment of bone infection, actively en ...201328811436
honeybee (apis mellifera)-associated bacterial community affected by american foulbrood: detection of paenibacillus larvae via microbiome analysis.honeybee (apis mellifera l.) workers act as passive vectors of paenibacillus larvae spores, which cause the quarantine disease american foulbrood (afb). we assessed the relative proportions of p. larvae within the honeybee microbiome using metabarcoding analysis of the 16 s rrna gene. the microbiome was analyzed in workers outside of the afb zone (control - afb0), in workers from asymptomatic colonies in an afb apiary (afb1), and in workers from colonies exhibiting clinical afb symptoms (afb2). ...201728698604
vancomycin-resistant enterococci with vana gene in treated municipal wastewater and their association with human hospital strains.vancomycin-resistant enterococci (vre) are pathogens of increasing medical importance. in brno, czech republic, we collected 37 samples from the effluent of a wastewater treatment plant (wwtp), 21 surface swabs from hospital settings, and 59 fecal samples from hospitalized patients and staff. moreover, we collected 284 gull cloacal swabs from the colony situated 35km downstream the wwtp. samples were cultured selectively. enterococci were identified using maldi-tof ms, phenotypically tested for ...201728763660
the potential of alcohol release doorplates to reduce surface contamination during hand contact.optimal hand hygiene may be compromised by contact with contaminated environmental surfaces.201728760635
inhibition of clostridium difficile in mice using a mixture of potential probiotic strains enterococcus faecalis nm815, e. faecalis nm915, and e. faecium nm1015: novel candidates to control c. difficile infection (cdi).this study is aimed at the isolation, identification, and characterization of potential probiotic strains capable of inhibiting clostridium difficile in vitro and in vivo. twenty isolates were isolated from infant fecal samples and screened against c. difficile using their cell-free supernatant. only three isolates showed maximum inhibition from 56.05 to 60.60%, thus they were characterized for probiotic properties and safety. the results obtained approved their tolerance to the gastrointestinal ...201728497217
eco-friendly fabrication of ag nanostructures using the seed extract of pedalium murex, an ancient indian medicinal plant: histopathological effects on the zika virus vector aedes aegypti and inhibition of biofilm-forming pathogenic bacteria.the control of zika virus mosquito vectors and well as the development of drugs in the fight against biofilm-forming microbial pathogens, are timely and important challenges in current bionanoscience. here we focused on the eco-friendly fabrication of ag nanostructures using the seed extract of pedalium murex, an ancient indian medicinal plant. initial confirmation of ag nanoparticles (agnps) production was showed by a color change from transparent to dark brown. the uv-visible spectrum (476nm), ...201728772238
microbial analysis of root canal and periradicular lesion associated to teeth with endodontic failure.the quantification of ten microorganisms at the root ends and in the surrounding periradicular lesions was performed. thirty 3 mm samples root ends and 30 samples of the surrounding chronic periapical infection were collected during apical microsurgery. samples were triturated, and the bacterial dna was obtained. the bacterial quantification was performed by using the sybr green system. at least one microorganism was detected in all patients. in both the root end and periapical samples, fusobact ...201728666877
antibiotic activity of emerimicin iv isolated from emericellopsis minima from talcahuano bay, chile.due to the increasing emergence of resistance of bacterial pathogens to current antibiotics, we have examined the marine fungi present in sea sediments obtained 200 m offshore to discover new antibacterial compounds active against multidrug-resistant bacteria. one strain, identified as emericellopsis minima, was isolated from sediments of talcahuano bay (chile). from the liquid culture of e. minima, we isolated emerimicin iv, a unique fungal peptaibol that exhibited antibacterial activity. the s ...201728669240
conjugal transfer of aac(6')ie-aph(2″)ia gene from native species and mechanism of regulation and cross resistance in enterococcus faecalis mcc3063 by real time-pcr.high level aminoglycoside resistance (hlar) in the lactic acid bacteria (lab) derived from food animals is detrimental. the aim of this study was to investigate the localization and conjugal transfer of aminoglycoside resistance genes, aac(6')ie-aph(2″)ia and aph(3')iiia in different enterococcus species. the cross resistance patterns in enterococcus faecalis mcc3063 to clinically important aminoglycosides by real time pcr were also studied. southern hybridization experiments revealed the presen ...201728774859
bloodstream infections caused by enterococcus spp: a 10-year retrospective analysis at a tertiary hospital in order to discover the risk factors for 30-day mortality in bloodstream infections (bsi) caused by enterococcus spp. strains, we explored the clinical and therapeutic profile of patients with enterococcus spp. bsi and the characteristics of this condition. a total of 64 patients with bsi caused by enterococcus spp. who were treated in our hospital between 2006 and 2015 were included in the study. the clinical features of patients, microbiology, and 30-day mortality were collected from the elec ...201728397040
the individual contribution of starter and non-starter lactic acid bacteria to the volatile organic compound composition of caciocavallo palermitano cheese.the contribution of two starter (lactobacillus delbrueckii and streptococcus thermophilus) and nine non-starter (enterococcus casselliflavus, enterococcus faecalis, enterococcus durans, enterococcus gallinarum, lactobacillus casei, lactobacillus paracasei, lactobacillus rhamnosus, pediococcus acidilactici and pediococcus pentosaceus) species of lactic acid bacteria (lab) to the volatile organic compounds (vocs) of caciocavallo palermitano cheese was investigated. the strains used in this study w ...201728783535
diversity of antagonistic bacteria isolated from medicinal plant peganum harmala l.the antimicrobial activity of plant extract of peganum harmala, a medicinal plant has been studied already. however, knowledge about bacterial diversity associated with different parts of host plant antagonistic to different human pathogenic bacteria is limited. in this study, bacteria were isolated from root, leaf and fruit of plant. among 188 bacterial isolates isolated from different parts of the plant only 24 were found to be active against different pathogenic bacteria i.e. escherichia coli ...201728855824
effect of conventional irrigation and photoactivated disinfection on enterococcus faecalis in root canals: an in vitro study.a study was done to evaluate the antimicrobial efficacy of sodium hypochlorite (naocl) and photoactivated disinfection (pad) on enterococcus faecalis.201728855761
enhancing antibacterial effect of sodium hypochlorite by low electric current-assisted sonic agitation.this research focused on the effects of low electric current (μe)-assisted sonic agitation of sodium hypochlorite on enterococcus faecalis infected human root dentin.201728854274
discovery of phylloseptins that defense against gram-positive bacteria and inhibit the proliferation of the non-small cell lung cancer cell line, from the skin secretions of phyllomedusa frogs.the growing occurrence of bacterial resistance to conventional antibiotics has called for the development of new classes of antimicrobial agents. antimicrobial peptides (amps) with broad antimicrobial spectrum derived from frog skin secretions have been demonstrated to be promising candidates for new antibiotic development. a proven rich source of these compounds are the skin secretions of the frogs in the phyllomedusa genus. in this study, two novel phylloseptin peptides-phylloseptin-pta and ph ...201728850103
infectious endocarditis from enterococcus faecalis associated with tubular adenoma of the sigmoid colon.enterococcus faecalis (e. faecalis), a constituent of the gut microbiota, can be associated with both colonic lesions and endocarditis. since this microorganism is one of the endocarditis etiological agents, there is a need for greater study in regard to the association with endocarditis and colonic lesions.201728848681
molecular determinants of the matrix thickening of a dual-species pseudomonas aeruginosa and enterococcus faecalis biofilm.biofilms are microbial communities that inhabit various surfaces and are surrounded by extracellular matrices (ecms). clinical microbiologists have shown that the majority of chronic infections are caused by biofilms, after introduction of the first biofilm infection model by j.w. costerton and his colleagues (1). however, treatments for chronic biofilm infections are still limited to surgical removal of the infected sites. pseudomonas aeruginosa and enterococcus faecalis are two frequently iden ...201728842537
how proteases from enterococcus faecalis contribute to its resistance to short α-helical antimicrobial peptides.hyl-20 (gilsslwkklkkiiak-nh2) is an analogue of a natural antimicrobial peptide (amp) previously isolated from the venom of wild bee. we examined its antimicrobial activity against three strains of enterococcus faecalis while focusing on its susceptibility to proteolytic degradation by two known proteases-gelatinase (gele) and serine protease (spre)-which are secreted by these bacterial strains. we found that hyl-20 was primarily deamidated at its c-terminal which made the peptide susceptible to ...201728830077
small alarmone synthetases as novel bacterial rna-binding proteins.the alarmone nucleotides guanosine pentaphosphate (pppgpp) and tetraphosphate (ppgpp), collectively referred to as (p)ppgpp, are key regulators of bacterial growth, stress adaptation, antibiotic tolerance and pathogenicity. we have recently shown that the small alarmone synthetase (sas) relq from the gram-positive pathogen enterococcus faecalis has an rna-binding activity (beljantseva et al. 2017). relq's activities as an enzyme and as a rna-binding protein are mutually incompatible: binding of ...201728820325
[elimination of entercoccus faecalis with different disinfection methods in root canals in vitro].to compare the elimination effect against e.faecalis in root canals with different methods.201728815239
antibacterial properties of nonwoven wound dressings coated with manuka honey or methylglyoxal.manuka honey (mh) is used as an antibacterial agent in bioactive wound dressings via direct impregnation onto a suitable substrate. mh provides unique antibacterial activity when compared with conventional honeys, owing partly to one of its constituents, methylglyoxal (mgo). aiming to investigate an antibiotic-free antimicrobial strategy, we studied the antibacterial activity of both mh and mgo (at equivalent mgo concentrations) when applied as a physical coating to a nonwoven fabric wound dress ...201728813014
in vitro antiplasmodial activity and prophylactic potentials of extract and fractions of trema orientalis (linn.) stem bark.trema orientalis (t. orientalis linn) has been used in the management of malaria in the western part of nigeria and despite its application in ethnomedicine, there is dearth of scientific evidence to justify the acclaimed prophylactic antimalarial usage of the plant. the aim of this study is to assess the in vitro antiplasmodial cell-free assay and chemopreventive efficacy of the methanol extract of the stem bark of t. orientalis and its fractions as a prophylactic regimen for malaria prevention ...201728810849
growth- and stress-induced pasta kinase phosphorylation in enterococcus faecalis.transmembrane ser/thr kinases containing extracellular pasta domains are ubiquitous among actinobacteria and firmicutes. such pasta kinases regulate critical processes including antibiotic resistance, cell division, toxin production, virulence, and are essential for viability in certain organisms. based on in vitro studies with purified extra- and intracellular fragments of pasta kinases, a model for signaling has been proposed, in which the extracellular pasta domains bind as-yet-undefined liga ...201728808126
genomic insights into the pathogenicity and environmental adaptability of enterococcus hirae r17 isolated from pork offered for retail sale.genetic information about enterococcus hirae is limited, a feature that has compromised our understanding of these clinically challenging bacteria. in this study, comparative analysis was performed of e. hirae r17, a daptomycin-resistant strain isolated from pork purchased from a retail market in beijing, china, and three other enterococcal genomes (enterococcus faecium do, enterococcus faecalis v583, and e. hirae atcc(™) 9790). some 1,412 genes were identified that represented the core genome t ...201728799224
methyl propiolate and 3-butynone: starting points for synthesis of amphiphilic 1,2,3-triazole peptidomimetics for antimicrobial evaluation.a library of 29 small 1,4-substituted 1,2,3-triazoles was prepared for studies of antimicrobial activity. the pharmacophore model investigated with these substrates was based on small peptidomimetics of antimicrobial peptides and antimicrobials isolated from marine organisms from sub-arctic regions. using methyl 1,2,3-triazole-carboxylates and 1,2,3-triazole methyl ketones prepared through "click" chemistry we were able to synthesize the different cationic amphiphiles through three steps or less ...201728797773
media from macrophages co-incubated with enterococcus faecalis induces epithelial cell monolayer reassembly and altered cell morphology.signal exchange between intestinal epithelial cells, microbes and local immune cells is an important mechanism of intestinal homeostasis. given that intestinal macrophages are in close proximity to both the intestinal epithelium and the microbiota, their pathologic interactions may result in epithelial damage. the present study demonstrates that co-incubation of murine macrophages with e. faecalis strains producing gelatinase (gele) and serine protease (spre) leads to resultant condition media ( ...201728793333
anti-enterococcal activities of pentacyclic triterpenes.asiatic (aa) and ursolic (ua) acids are widely studied phytochemicals, but their antimicrobial properties are still poorly understood. therefore our research has focused on their activity against uropathogenic enterococcus faecalis strains.201728791824
characterization of the tertiary structure of the peptidoglycan of enterococcus faecalis.solid-state nmr spectra of whole cells and isolated cell walls of enterococcus faecalis grown in media containing combinations of (13)c and (15)n specific labels in d- and l-alanine and l-lysine (in the presence of an alanine racemase inhibitor alaphosphin) have been used to determine the composition and architecture of the cell-wall peptidoglycan. the compositional variables include the concentrations of (i) peptidoglycan stems without bridges, (ii) d-alanylated wall teichoic acid, (iii) cross- ...201728784459
coupling metagenomics with cultivation to select host-specific probiotic microorganisms for subtropical demonstrate a non-empirical workflow to select host-specific probiotics for aquaculture industry.201728783248
septic episodes in a premature infant after in utero exposure to rituximab.rituximab is an increasingly used immunotherapeutic agent for women of reproductive age for treatment of autoimmune diseases, leukemias, and lymphomas. rituximab is a chimeric monoclonal antibody that targets b-cell surface antigen cd20 and can cross the placenta. current evidence of the impact of this medication on the developing fetus is limited, but there is little to suggest that fetal exposure to this medication places an infant at increased risk of immunosuppression and subsequent infectio ...201728768852
microbial load in septic and aseptic procedure rooms.highly effective measures to prevent surgical wound infections have been established over the last two decades. we studied whether the strict separation of septic and aseptic procedure rooms is still necessary.201728764834
fabrication and appraisal of poly (lactic-co-glycolic acid) - moxifloxacin nanoparticles using vitamin e-tpgs: a potential intracanal drug delivery agent.nanotechnology is gaining momentum in the search for ideal biomaterials by dental researchers. the expansible nature of endodontology deems exploration, learning and scrutinizing newer avenues which have the potential to be applied and investigated. the popularity of polymers as drug delivery vehicles has opened avenues for their application in the root canal system. this study utilized application of biodegradable polymers as potential drug delivery vehicles against enterococcus faecalis, one o ...201728764283
revisiting nitrofurantoin for vancomycin resistant enterococci.enterococcal infection has emerged as a major therapeutic challenge. emergence of high level aminoglycoside resistance (hlar) and vancomycin-resistant enterococcus (vre) has further limited the drug therapy in enterococcal infections. however, nitrofuratoin being an old drug reported to have less resistance in comparison to the other classes of antimicrobial agents.201728764160
additive effect of photoactivated disinfection on the antibacterial activity of qmix 2in1 against 6-week enterococcus faecalis biofilms: an in vitro study.evaluation of the additive effect of photodynamic therapy (pdt) on the antibacterial activity of 2.5% sodium hypochlorite (naocl) and qmix against 6-week enterococcus faecalis biofilms contaminated root canals.201728761252
first case of multidrug-resistant enterococcus faecalis isolated from herding horse in xinjiang, western china. 201728760414
in vitro synergistic activities of cefazolin and nisin a against mastitis pathogens.first-generation cephalosporins such as cefazolin (cez) have been widely used for mastitis treatment in dairy cattle. however, the use of antibiotics results in the presence of antibiotic residues in milk, which is used for human consumption. nisin a, a bacteriocin produced by lactococcus lactis, has been used as a broad-spectrum food preservative for over 50 years. therefore, a combination of cez and nisin a might provide an extended activity spectrum against mastitis pathogens and reduce the a ...201728757508
efficacy of photon-induced photoacoustic streaming in the reduction of enterococcus faecalis within the root canal: different settings and different sodium hypochlorite concentrations.the purpose of this study was to determine the effectiveness of laser-activated irrigation by photon-induced photoacoustic streaming (pips) in the reduction of enterococcus faecalis in root canal disinfection, varying laser energy output, and sodium hypochlorite (naocl) concentration. for effective removal of the smear layer, the sequence and resting time of the final irrigation steps were modified compared with the standard pips protocol.201728756961
draft genome sequence of enterococcus faecalis dd14, a bacteriocinogenic lactic acid bacterium with anti-clostridium activity.we report the draft genome sequence of enterococcus faecalis dd14, a strain isolated from meconium of a healthy newborn at roubaix hospital (france). the strain displayed antagonism against a set of gram-positive bacteria through concomitant production of lactic acid and bacteriocin. the genome has a size of 2,893,365 bp and a 37.3% g+c ratio and is predicted to contain at least 2,755 coding sequences and 62 rnas.201728751398
photodynamic therapy with high-power led mediated by erythrosine eliminates enterococcus faecalis in planktonic forms.the failure of endodontic treatment is linked to the presence of microorganisms, particularly enterococcus faecalis, in the root canals. this study evaluated the effectiveness of photodynamic therapy (pdt) using erythrosine irradiated by a high-power curing light on a planktonic suspension culture of e. faecalis.201728750753
buwchitin: a ruminal peptide with antimicrobial potential against enterococcus faecalis.antimicrobial peptides (amps) are gaining popularity as alternatives for treatment of bacterial infections and recent advances in omics technologies provide new platforms for amp discovery. we sought to determine the antibacterial activity of a novel antimicrobial peptide, buwchitin, against enterococcus faecalis. buwchitin was identified from a rumen bacterial metagenome library, cloned, expressed and purified. the antimicrobial activity of the recombinant peptide was assessed using a broth mic ...201728748180
assessment of facultative anaerobes from the root canals of deciduous molars: an in vivo study.background. the current research aimed to assess the prevalence of facultative anaerobes isolated from the root canals of deciduous molars. methods. the present research enrolled 60 children in the 6‒9-year age group based on clinical and radiographic findings. under aseptic conditions, access cavities were prepared followed by collection of samples from infected root canals with the help of sterile paper points. the samples thus obtained were subjected for microbial assay. results. it was found ...201728748050
effect of different mixing methods on the bacterial microleakage of white portland cement and white mineral trioxide aggregate.background. of this study was to investigate the effect of different mixing methods (ultrasonic, amalgamator, and conventional) on the bacterial microleakage of white portland cement (wpc) and white mta (tooth-colored formula, dentsply, tulsa dental, tulsa, ok). methods. a hundred human single-rooted permanent teeth were decoronated to obtain 14 mm of root length in all the samples. the root canals were cleaned, shaped and obturated. three millimeters of each root apex were cut off and randomly ...201728748048
determination of disk diffusion and mic quality control ranges for nafithromycin (wck 4873), a new lactone-ketolide.disk diffusion and mic quality control (qc) ranges were determined for nafithromycin, a new lactone-ketolide, following completion of a nine-laboratory, clinical and laboratory standards institute (clsi) m23-defined tier 2 study. five qc strains consistent with the spectrum of activity of nafithromycin were tested: staphylococcus aureus atcc 25923 (disk only), s. aureus atcc 29213 (broth only), enterococcus faecalis atcc 29212 (broth only), streptococcus pneumoniae atcc 49619 (disk and broth), a ...201728747372
bacterial size matters: multiple mechanisms controlling septum cleavage and diplococcus formation are critical for the virulence of the opportunistic pathogen enterococcus faecalis.enterococcus faecalis is an opportunistic pathogen frequently isolated in clinical settings. this organism is intrinsically resistant to several clinically relevant antibiotics and can transfer resistance to other pathogens. although e. faecalis has emerged as a major nosocomial pathogen, the mechanisms underlying the virulence of this organism remain elusive. we studied the regulation of daughter cell separation during growth and explored the impact of this process on pathogenesis. we demonstra ...201728742152
characterization of enterococcus faecalis isolates by chicken embryo lethality assay and eric-pcr.enterococcus faecalis is the major causative agent of amyloid arthropathy in chickens. given the difficulty of estimating the risk from field strains, the embryo lethality assay (ela) is proposed in this study as a model to predict the virulence of 68 avian e. faecalis strains. additionally, enterobacterial repetitive intergenic consensus polymerase chain reaction (eric-pcr) was used to characterize the genetic diversity of the e. faecalis strains. the ela was performed 10 times with subsets of ...201728737107
genetic diversity and antimicrobial resistance profile of enterococcus faecalis isolated from broilers with vertebral osteomyelitis in southeast brazil.vertebral osteomyelitis (vo) is a worldwide emerging disease that affects broilers. recently, the isolation of enterococcus faecalis in cases of the disease has been described. this study aimed at determining the genetic diversity and antimicrobial resistance profile of 12 enterococcus faecalis strains isolated from broilers with vo. strains were isolated from nine flocks from six farms in a high-density poultry production area in southeast brazil and were evaluated using multilocus sequence typ ...201728737063
the usefulness of biosynthetic vascular graft omniflow ii and autologous veins for the treatment of massive infection of dacron vascular graft with enterococcus faecalis hlar.infections of vascular grafts are the most severe complications in vascular surgery. we present the case of a 73-year-old male with infection of a dacron prosthesis with a strain of enterococcus faecalis. the patient was treated with replacement of a full prosthesis with a combined graft constructed from biosynthetic vascular graft omniflow and autologous veins. this graft is recommended for implantation in patients with a higher risk of infection. our case is one of the first reported usage of ...201728735332
activity of dalbavancin tested against gram-positive clinical isolates causing skin and skin structure infections in paediatric patients from us hospitals (2014 - 2015).this study provides an in vitro analysis of dalbavancin activity against isolates causing skin and skin structure infections (sssis) in children.201728735053
telavancin activity in vitro tested against a worldwide collection of gram-positive clinical isolates (2014).telavancin activity was measured against gram-positive pathogens collected worldwide during 2014 using the revised broth microdilution testing method. the results were compared with previous reports.201728735051
in vitro stepwise selection of reduced susceptibility to lipoglycopeptides in enterococci.the propensity of oritavancin to select for stably elevated oritavancin minimum inhibitory concentrations (mics) was studied by serial passaging of strains in broth containing oritavancin for 20days. seven clinical strains of enterococcus faecalis and e. faecium were studied; they included vancomycin-susceptible and both vana and vanb vancomycin-resistant isolates. stepwise oritavancin selection yielded stably elevated oritavancin mics in six of the seven strains, with mic increases ranging from ...201728733126
hypochlorite solution for root canal irrigation that lacks a chlorinated odor.this is an in vitro study to develop a formulation of a hypochlorite solution for root canal irrigation that lacks a chlorinated odor. the antibacterial effect, tissue dissolution efficacy, and the cytotoxicity of the solution were assessed in cell culture and were compared with those of commercial sodium hypochlorite (naocl) solutions.201728729797
in vitro activity of telavancin against clinically important gram-positive pathogens from 69 u.s. medical centers (2015): potency analysis by u.s. census divisions.a total of 8,072 gram-positive isolates collected from 69 medical centers among all 9 u.s. census divisions during the 2015 sentry antimicrobial surveillance program were included. telavancin had minimal inhibitory concentration (mic)50 and mic90 values of 0.03/0.06 μg/ml, respectively, against methicillin-susceptible (mssa) and methicillin-resistant staphylococcus aureus (mrsa). similar activity was also observed among coagulase-negative staphylococci (mic50/90, 0.03/0.06 μg/ml; 100.0% inhibite ...201728727951
soymilk fermentation by enterococcus faecalis vb43 leads to reduction in the immunoreactivity of allergenic proteins β-conglycinin (7s) and glycinin (11s).food allergies represent a serious problem affecting human health and soy proteins rank among the most allergenic proteins from food origin. the proteolytic enzymes produced by lactic acid bacteria (lab) can hydrolyse the major allergens present in soybean, reducing their immunoreactivity. many studies have reported the ability of lab to ferment soy-based products; while the majority of them focus on the improvement of the sensory characteristics and functionality of soy proteins, a lack of info ...201728726509
integrating highly quantitative proteomics and genome-scale metabolic modeling to study ph adaptation in the human pathogen enterococcus faecalis.genome-scale metabolic models represent the entirety of metabolic reactions of an organism based on the annotation of the respective genome. these models commonly allow all reactions to proceed concurrently, disregarding the fact that at no point all proteins will be present in a cell. the metabolic reaction space can be constrained to a more physiological state using experimentally obtained information on enzyme abundances. however, high-quality, genome-wide protein measurements have been chall ...201628725473
antibacterial effect of azadirachta indica (neem) or curcuma longa (turmeric) against enterococcus faecalis compared with that of 5% sodium hypochlorite or 2% chlorhexidine in vitro.the purpose of this study was to compare the antibacterial properties of azadirachta indica (neem) or curcuma longa (turmeric) against enterococcus faecalis with those of 5% sodium hypochlorite or 2% chlorhexidine as root canal irrigants in vitro. the activity of neem, chlorhexidine, sodium hypochlorite, or turmeric against e. faecalis was measured on agar plates using the agar diffusion method. the tube dilution method was used to determine the minimum inhibitory concentration (mic) and minimum ...201728724858
biofilm formation in enterococci: genotype-phenotype correlations and inhibition by vancomycin.enterococci are nosocomial pathogens that can form biofilms, which contribute to their virulence and antibiotic resistance. although many genes involved in biofilm formation have been defined, their distribution among enterococci has not been comprehensively studied on a genome scale, and their diagnostic ability to predict biofilm phenotypes is not fully established. here, we assessed the biofilm-forming ability of 90 enterococcal clinical isolates. major patterns of virulence gene distribution ...201728720810
virulence genes in biofilm producer enterococcus faecalis isolates from root canal infections.enterococcus faecalis is occurring in opportunistic infections involving the oral cavity. this study aimed to evaluate the presence of e. faecalis virulence genes in dental root canal isolates recovered from advanced chronic periodontitis patients. one hundred e. faecalis isolated from dental root canal during july 2015 to oct 2016. after analysis of biofilm formation by the semi-quantitative determination in 96-well flat bottom polystyrene plates, the presence of asa, esp, efaa, ace, ebpr, gel ...201728719346
screening of oxalate degrading lactic acid bacteria of food origin.a screening for oxalate degrading abilities was initially carried on within lactic acid bacteria cultures of different food origin. seventy-nine strains were drop-inoculated onto mrs agar plates containing calcium oxalate. by comparing colonies diameters, 31 strains were used to inoculate, in parallel, mrs and mrs modified by sodium oxalate addition. differences in the strains' growth were assessed by colony forming unit counts. for two strains, the growth in oxalate enriched medium was signific ...201728713789
genetic characterisation confirms sporadic occurrence of vancomycin-resistant enterococcus faecalis in copenhagen, denmark. 201728709988
short-term alcohol abstinence improves antibacterial defenses of chronic alcohol-consuming mice against gut bacteria-associated sepsis caused by enterococcus faecalis oral infection.the effects of short-term alcohol abstinence on host antibacterial resistance against enterococcus faecalis oral infection was investigated in chronic alcohol-consuming mice [mice with 0.1 g/day of 20% ethanol consumption for 12 or 16 weeks (cac-mice)]. these mice were highly susceptible to the infection; however, after 7 days of alcohol abstinence (aacac-mice), their antibacterial resistances were completely restored to the normal mouse level. normal mice inoculated with cac-mouse hepatic macro ...201728708971
virulence and antibiotic resistance of enterococci isolated from healthy breastfed infants.pathogenic ability has been extensively studied in clinical enterococci, but to a lesser extent in community-derived ones. most studies to date in enterococci from healthy infants have been focused on enterococcus faecalis, despite the growing concern about nosocomial infections caused by e. faecium. in this work, we studied the antibiotic resistance and virulence determinants of 26 e. faecalis and 15 e. faecium intestinal isolates from spanish healthy breastfed infants. overall, commensal enter ...201728708453
evaluation of free or anchored antimicrobial peptides as candidates for the prevention of orthopaedic device-related infections.the prevention of implant-associated infection, one the most feared complications in orthopaedic surgery, remains a major clinical challenge and urges development of effective methods to prevent bacterial colonization of implanted devices. alpha-helical antimicrobial peptides (amps) may be promising candidates in this respect due to their potent and broad-spectrum antimicrobial activity, their low tendency to elicit resistance and possible retention of efficacy in the immobilized state. the aim ...201728707817
postoperative enterococcus faecalis endophthalmitis: virulence factors leading to poor visual outcome.purpose postoperative endophthalmitis caused by enterococcus faecalis often results in poor visual outcome due to retinal destruction. we evaluated the virulence factors of enterococcus faecalis clinical isolates and analyzed their relationship with retinal destruction and poor visual outcomes in postoperative bacterial endophthalmitis (pbe) following cataract surgery.201728707018
characterization and application of antilisterial enterocins on model fresh cheese.enterococcus faecalis strains isolated from an artisanal cheese were selected based on enterocin production against listeria monocytogenes. the strains formed biofilms and presented high hydrophobic character and good autoaggregation and coaggregation capacity with l. monocytogenes. strains l3a21m3 and l3b1k3 presented high survival under gastrointestinal conditions, were able to adhere to human intestinal cells (caco-2 and ht-29), and blocked the adhesion and invasion of l. monocytogenes. the a ...201728703625
restructuring of enterococcus faecalis biofilm architecture in response to antibiotic-induced stress.bacterial biofilms are intrinsically resistant to antimicrobial treatment, which contributes to microbial persistence in clinical infections. enterococcus faecalis is an opportunistic pathogen that readily forms biofilms and is the most prevalent enterococcal species identified in healthcare-associated infections. since intrinsic resistance to multiple antibiotics is common for enterococci, and antibiotic resistance is elevated in biofilm populations, it is imperative to understand the mechanism ...201728685097
draft genome sequence of enterococcus faecalis atcc baa-2128.while a part of the native gut microflora, the gram-positive bacterium enterococcus faecalis can lead to serious infections elsewhere in the body. the draft genome of e. faecalis strain atcc baa-2128, isolated from piglet feces, was examined. this draft genome consists of 42 contigs, 12 of which exhibit homology to annotated plasmids.201728684576
role of purified β-1, 3 glucan binding protein (β-gbp) from paratelphusa hydrodromus and their anti-inflammatory, antioxidant and antibiofilm properties.β- 1, 3-glucan binding protein (β-gbp), a pattern recognition protein (prp), plays a critical role in triggering the innate immune response by detecting β-glucan found on the surface of microbes. in the present study, β-gbp was purified from the haemolymph of rice field crab paratelphusa hydrodromus by affinity column chromatography. the monomeric protein ph-β-gbp appeared as a single band with a molecular weight of approximately 95 kda in sds-page analysis and its purity was determined to be 89 ...201728684323
incidence, clinical characteristics, and outcomes of nosocomial enterococcus spp. bloodstream infections in a tertiary-care hospital in beijing, china: a four-year retrospective study.enterococcus spp. are the common cause of nosocomial bloodstream infections (bsis) with high morbidity and mortality. the purpose of this study was to characterize the incidence, clinical and microbiological features, and mortality of nosocomial enterococcal bsis at a large chinese tertiary-care hospital in beijing, china.201728680588
the enterococcus faecalis virulence factor elra interacts with the human four-and-a-half lim domains protein 2.the commensal bacterium enterococcus faecalis is a common cause of nosocomial infections worldwide. the increasing prevalence of multi-antibiotic resistant e. faecalis strains reinforces this public health concern. despite numerous studies highlighting several pathology-related genetic traits, the molecular mechanisms of e. faecalis virulence remain poorly understood. in this work, we studied 23 bacterial proteins that could be considered as virulence factors or involved in the enterococcus inte ...201728676674
stochasticity in the enterococcal sex pheromone response revealed by quantitative analysis of transcription in single enterococcus faecalis, sex pheromone-mediated transfer of antibiotic resistance plasmids can occur under unfavorable conditions, for example, when inducing pheromone concentrations are low and inhibiting pheromone concentrations are high. to better understand this paradox, we adapted fluorescence in situ hybridization chain reaction (hcr) methodology for simultaneous quantification of multiple e. faecalis transcripts at the single cell level. we present direct evidence for variability in the ...201728671948
carriage of multidrug resistant enterococcus faecium and enterococcus faecalis among apparently healthy humans.enterococci are indigenous flora of the gastro-intestinal tracts of animals and humans. recently, interest in two major species, e. faecium and e. faecalis, has heightened because of their ability to cause serious infections and their intrinsic resistance to antimicrobials. this study was aimed at determining the prevalence of e. faecium and e. faecalis in human faecal samples and evaluating the susceptibility of the isolates to antibiotics.201728670644
prevalence and genetic diversity of enterococcus faecalis isolates from mineral water and spring water in china.enterococcus faecalis is an important opportunistic pathogen which is frequently detected in mineral water and spring water for human consumption and causes human urinary tract infections, endocarditis and neonatal sepsis. the aim of this study was to determine the prevalence, virulence genes, antimicrobial resistance and genetic diversity of e. faecalis from mineral water and spring water in china. of 314 water samples collected from january 2013 to january 2014, 48 samples (15.3%) were contami ...201728670302
determining the optimal dose of 1940-nm thulium fiber laser for assisting the endodontic treatment.insufficient cleaning, the complex anatomy of the root canal system, inaccessible accessory canals, and inadequate penetration of irrigants through dentinal tubules minimizes the success of the conventional endodontic treatment. laser-assisted endodontic treatment enhances the quality of conventional treatment, but each laser wavelength has its own its own limitations. the optimal parameters for the antibacterial efficiency of a new wavelength, 1940-nm thulium fiber laser, were firstly investiga ...201728669067
urinary tract infections after kidney transplantation: a risk factor analysis of 417 patients.background the aim of this study was to evaluate the number of urinary tract infections (uti) that occur after kidney transplantation (kt) and to identify possible risk factors for development of a uti. material and methods we retrospectively analyzed all kts performed between january 2012 and december 2013 in the erasmus university medical center, rotterdam. uti was scored if: (1) a patient had a urine culture with no more than 2 species of microorganisms, (2) at least 1 of which was a bacteriu ...201728663538
altered antibacterial activity of curcumin in the presence of serum albumin, plasma and whole blood.antibacterial effect is one of the major therapeutic activities of plant-derived curcumin. this work evaluated the effect of serum albumin, human plasma, and whole blood on the in vitro activity of curcumin against eight clinical bacterial isolates by standard broth microdilution and plate-counting methods. toxicological effects of curcumin towards human red blood cells (rbcs) and peripheral blood mononuclear cells (pbmcs) were also investigated. curcumin exhibited weak activity against gram-neg ...201728649069
[mitral valve endocarditis diagnosed after panophthalmitis with enterococcus faecalis].an 86-year-old man underwent cataract surgery on the left eye, but suddenly he lost his vision three days after surgery. topical treatments for panophthalmitis were started according to international guidelines. later, a broad-range polymerase chain reaction showed enterococcus faecalis in corpus vitreum, and iv antiobiotic treatment was initiated. to exclude endocarditis an echocardiography was performed, and this showed mitral regurgitation with a small mobile vegetation. uncertainty concernin ...201728648168
antibacterial effect of genetically-engineered bacteriophage ϕef11/ϕfl1c(δ36)p(nisa) on dentin infected with antibiotic-resistant enterococcus faecalis.enterococcus faecalis is a gram-positive facultative anaerobic bacterium, which is present in 30-89% of teeth with postendodontic treatment failures. e. faecalis is capable of penetrating dentinal tubules and surviving as a monoculture after conventional endodontic therapy, indicating that it is resistant to commonly used endodontic disinfection protocols. different e. faecalis strains have shown resistance to several antibiotics, and have been associated with both dental pathology and systemic ...201728646693
phtβ-promoted mobilization of non-conjugative resistance plasmids from enterococcus faecium to enterococcus analyse the recombination events associated with conjugal mobilization of two multiresistance plasmids, prum17i48 and plag (formerly named pdo1-like), from enterococcus faecium 17i48 to enterococcus faecalis jh2-2.201728645197
methacrylate-based root canal sealer containing chlorexidine and α-tricalcium phosphate.the aim of this study was to develop and to characterize a methacrylate-based root canal sealer containing chlorhexidine (chx) and α-tricalcium phosphate (α-tcp). experimental dual cure methacrylate-based sealer was produced containing 0, 2.5, or 5 wt% of chx and 0, 25, or 50 wt% of α-tcp. experimental sealers were evaluated based on flow, film thickness, radiopacity, degree of conversion (dc), degradation in water, ph and antibacterial activity. flow ranged from 15.09 ± 0.11 to 17.47 ± 0.42 mm. ...201728644564
exploiting crispr-cas to manipulate enterococcus faecalis populations.crispr-cas provides a barrier to horizontal gene transfer in prokaryotes. it was previously observed that functional crispr-cas systems are absent from multidrug-resistant (mdr) enterococcus faecalis, which only possess an orphan crispr locus, termed crispr2, lacking cas genes. here, we investigate how the interplay between crispr-cas genome defense and antibiotic selection for mobile genetic elements shapes in vitro e. faecalis populations. we demonstrate that crispr2 can be reactivated for gen ...201728644125
the effect of gelatinase production of enterococcus faecalis on adhesion to dentin after irrigation with various endodontic irrigants.objectives: the aim of this study was to evaluate whether the gelatinase production ability of enterococcus faecalis provides any advantage on adhesion of this bacterium to dentin treated with various irrigants and their combinations. materials and methods: standardized dentin discs were randomly divided into five groups (n = 20): group 1: 2.5% sodium hypochlorite (naocl), group 2: 2% chlorhexidine (chx), group 3: naocl + saline + chx, group 4: naocl + edta + naocl, group 5: qmix. after incubati ...201628642925
comparative evaluation of anti microbial effects of triple antibiotic paste and amox and its derivatives against e. faecalis: an in vitro study.enterococcus faecalis is a microorganism commonly detected in asymptomatic, persistent endodontic infections. triple antibiotic paste has stood the test of time as a proven antibiotic combination against e. faecalis. however, problems with this include staining of teeth and standardization of the preparation. thus, the search for better alternatives and better preparation techniques is still on.201728638559
antibacterial activity of different root canal sealers against enterococcus faecalis.the aim of the present study was to compare in vitro the antimicrobial activity of different root canal sealers against enterococcus faecalis, prior and subsequent to setting.201728638549
telavancin: a novel semisynthetic lipoglycopeptide agent to counter the challenge of resistant gram-positive pathogens.telavancin (td-6424), a semisynthetic lipoglycopeptide vancomycin-derivative, is a novel antimicrobial agent developed by theravance for overcoming resistant gram-positive bacterial infections, specifically methicillin-resistant staphylococcus aureus (mrsa). the us food and drug administration (usfda) had approved telavancin in 2009 for the treatment of complicated skin and skin structure infections (csssis) caused by gram-positive bacteria, including mrsa (s. aureus, streptococcus agalactiae, s ...201728634536
antibacterial activity of crude extracts of some south african medicinal plants against multidrug resistant etiological agents of diarrhoea.this study evaluated the antibacterial activity of some plants used in folklore medicine to treat diarrhoea in the eastern cape province, south africa.201728629407
molecular identification of multiple antibiotic resistant fish pathogenic enterococcus faecalis and their control by medicinal herbs.the opportunistic fish pathogen, enterococcus faecalis has been reported to cause mass mortality in several fish species in different countries. the objectives of this study were to (i) identify e. faecalis from the diseased fishes through molecular techniques; (ii) assess the antibiotic susceptibility profile of e. faecalis isolates; and (iii) control disease in tilapia fish by treatment with medicinal plant extracts. a total of 48 isolates were phenotypically identified as enterococcus species ...201728623336
bactericidal effect of er:yag laser-activated sodium hypochlorite irrigation against biofilms of enterococcus faecalis isolate from canal of root-filled teeth with periapical lesions.this study was to evaluate the bactericidal effect of er:yag laser-activated sodium hypochlorite irrigation (er:yag + naocl) on biofilms of enterococcus faecalis clinical isolate.201728622484
design, synthesis, and evaluation of novel tyrosine-based dna gyrase b inhibitors.the discovery and synthesis of new tyrosine-based inhibitors of dna gyrase b (gyrb), which target its atpase subunit, is reported. twenty-four compounds were synthesized and evaluated for activity against dna gyrase and dna topoisomerase iv. the antibacterial properties of selected gyrb inhibitors were demonstrated by their activity against staphylococcus aureus and enterococcus faecalis in the low micromolar range. the most promising compounds, 8a and 13e, inhibited escherichia coli and s. aure ...201728621824
spectrum and antibiotic resistance of uropathogens between 2004 and 2015 in a tertiary care hospital in hungary.surveillance of the bacterial spectrum and antibiotic-resistance patterns of locally occurring uropathogens is essential to serve as a basis for empirical treatment of urinary tract infections (utis), as antibiotic-resistance rates may vary geographically with significant differences between countries and regions, and with time.201728621642
photodvnamic therapy in endodontics.commonly used irrigants do not always eradicate the entire microbial flora in infected root canals. therefore, several other strategies, such as photodynamic therapy (pdt) have been developed. photoactivated disinfection is based on the interaction of a photosensitive antibacterial agent and a light source. it uses a nontoxic dye named photosensitizer (ps) and low-intensity visible light. in oxygen presentation, these combine to produce some cytotoxic species. the ps molecules attach to bacteria ...201728621288
infectious crystalline keratopathy after descemet’s stripping endothelial keratoplasty.a 68-year-old woman presented with infectious crystalline keratopathy 4 months after she underwent a combined phacoemulsification and descemet's stripping endothelial keratoplasty for fuch's endothelial dystrophy in her left eye. after 5 months of topical moxifloxacin 1%, the infiltrate responded well but had not completely resolved, with the resulting endothelial failure requiring a penetrating keratoplasty 9 months after the initial operation. microbiology identified enterococcus faecalis with ...201728619977
draft genome sequences of six enterococcus faecalis strains isolated from malaysian clinical and environmental origins.enterococcus faecalis is known to cause a variety of nosocomial infections, including urinary tract infections. antibiotic resistance and virulence properties in this species are of public concern. the draft genome sequences of six e. faecalis strains isolated from clinical and environmental sources in malaysia are presented here.201728619812
evaluation of antimicrobial activity of association of chlorhexidine to photosensitizer used in photodynamic therapy in root canals infected by enterococcus faecalis.the aim of the present study was to evaluate, in vitro, the influence of the addition of chlorhexidine to photosensitiser in the antimicrobial activity of photodynamic therapy in root canals infected by enterococcus faecalis.201728619615
triterpenoid saponins and other glycosides from the stems and bark of jaffrea xerocarpa and their biological activity.six previously undescribed triterpenoid saponins and two previously undescribed norlupane triterpenes were isolated with five known saponins, three known lupane derivatives, 17,20-didehydro-20-deoxyjujubogenin, rutin, (±) 3α-o-β-d-glucopyranosyl-lyoniresinol, (±) 4-o-β-d-glucopyranosyl-maesopsin, three phenol glycosides, and uridine from the stems and bark of jaffrea xerocarpa (baill.) h. c. hopkins & pillon (= basionym alphitonia xerocarpus baill.) (rhamnaceae), an endemic tree of new caledonia ...201728614728
signal biosynthesis inhibition with ambuic acid as a strategy to target antibiotic-resistant infections.there has been major interest by the scientific community in antivirulence approaches against bacterial infections. however, partly due to a lack of viable lead compounds, antivirulence therapeutics have yet to reach the clinic. here we investigate the development of an antivirulence lead targeting quorum sensing signal biosynthesis, a process that is conserved in gram-positive bacterial pathogens. some preliminary studies suggest that the small molecule ambuic acid is a signal biosynthesis inhi ...201728607020
bacterial sepsis in neonates: single centre study in a neonatal intensive care unit in bosnia and herzegovina.the aim of the study was to evaluate the incidence, mortality, risk factors, aetiology and the susceptibility to antibiotics of the bacteria responsible for sepsis.201728605923
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