single consumption of bryndza cheese temporarily affects oral microbiota and salivary markers of oxidative stress.several oral diseases are associated with changes in oral microbiota and higher oxidative stress. enterococcus faecalis has been hypothesized to directly contribute to the oxidative stress in oral cavity. the aim of this study was to examine the effect of single consumption of unpasteurized bryndza cheese containing enterococci on changes of microbiota and oxidative status in saliva. fourteen healthy volunteers aged 23-30 years were asked to eat 100 g of bryndza cheese. saliva samples were colle ...201424997802
gestational age-specific reference ranges of hepcidin in cord blood.iron deficiency (id) contributes to anaemia of prematurity, and hence the reliable assessment of iron nutrition status appears to be mandatory.201424993575
stability of antibacterial activity of chlorhexidine and doxycycline in bovine root dentine.the aim of this study was to compare the substantivity of chlorhexidine (chx) and doxycycline bond to the dentin in diffusion disk method.201424991631
efficacy of coral-hydroxyapatite and biphasic calcium phosphate for early bacterial detection.nano- or microhydroxyapatites with microbiological properties are being used to detect pathogens in clinical samples and industrial environments. in this study, the calcium phosphates coral-hydroxyapatite and biphasic calcium phosphate were characterized physicochemically using x-ray diffraction, thermogravimetric, and differential thermal analysis. the morphology, texture, and chemical composition of the ceramics were also investigated using scanning electron microscopy with energy dispersive s ...201424985222
mechanisms of vancomycin resistance in staphylococcus aureus.vancomycin is a glycopeptide antibiotic used for the treatment of gram-positive bacterial infections. traditionally, it has been used as a drug of last resort; however, clinical isolates of methicillin-resistant staphylococcus aureus (mrsa) strains with decreased susceptibility to vancomycin (vancomycin intermediate-resistant s. aureus [visa]) and more recently with high-level vancomycin resistance (vancomycin-resistant s. aureus [vrsa]) have been described in the clinical literature. the rare v ...201424983424
molecular basis for bacterial peptidoglycan recognition by lysm domains.carbohydrate recognition is essential for growth, cell adhesion and signalling in all living organisms. a highly conserved carbohydrate binding module, lysm, is found in proteins from viruses, bacteria, fungi, plants and mammals. lysm modules recognize polysaccharides containing n-acetylglucosamine (glcnac) residues including peptidoglycan, an essential component of the bacterial cell wall. however, the molecular mechanism underpinning lysm-peptidoglycan interactions remains unclear. here we des ...201424978025
application of multilocus sequence analysis for molecular characterization of enterococci with virulence factors recovered from a tropical recreational beach.partial gene sequences of phenylalanyl-trna synthase alpha subunit (phes) and rna polymerase alpha subunit (rpoa) were evaluated for species delineation and detection of recombination among enterococci populations recovered from a bathing beach impacted by low tide river flow. at inter-species level, a maximum similarity of 86.5% and 94.8% was observed among the enterococci phes and rpoa sequence, respectively. a superimposed plot of delimited pairwise similarity values obtained for 266 pair-wis ...201424974655
[in vitro antimicrobial susceptibility in enterococcus faecalis and enterococcus faecium isolated from hospitalized patients].enterococcus spp is currently considered as one of the most important nosocomial pathogens . the antibiotic resistance of this group of bacteria is a particularly important problem in health centers.201424968036
the antimicrobial activity of azadirachta indica, mimusops elengi, tinospora cardifolia, ocimum sanctum and 2% chlorhexidine gluconate on common endodontic pathogens: an in vitro check the antimicrobial activity of azadirachta indica (neem), ocimum sanctum (tulsi), mimusops elelngi (bakul), tinospora cardifolia (giloy) and chlorhexidine gluconate (chx) on common endodontic pathogens like streptococcus mutans, enterococcus faecalis and staphylococcus aureus.201424966766
effect of "apical clearing" and "apical foramen widening" on apical ramifications and bacterial load in root canals.the purpose of the study was to determine the effect of apical clearing and apical foramen widening in reducing apical ramifications and bacterial load in the apical third of root canals. the mesio-buccal roots of 21 maxillary first molar teeth were inoculated with enterococcus faecalis suspension using a sterile pipette. samples were incubated at 37°c for 72 hrs and divided into 3 groups: group a, control group (n=5), no preparation; group b (n=8) conventional preparation alone; and group c (n= ...201424965951
prevalence of asymptomatic bacteriuria and its antibacterial susceptibility pattern among pregnant women attending the antenatal clinic at kanpur, india.symptomatic and asymptomatic bacteriuria (asb) is common in pregnant women. pregnancy enhances the progression from asb to symptomatic bacteriuria, which if left untreated, could lead to acute pyelonephritis and other adverse outcomes such as prematurity, postpartum, hypertensive disease, anaemia, utis and higher foetal mortality rates.201424959438
defining daptomycin resistance prevention exposures in vancomycin-resistant enterococcus faecium and e. faecalis.daptomycin is used off-label for enterococcal infections; however, dosing targets for resistance prevention remain undefined. doses of 4 to 6 mg/kg of body weight/day approved for staphylococci are likely inadequate against enterococci due to reduced susceptibility. we modeled daptomycin regimens in vitro to determine the minimum exposure to prevent daptomycin resistance (dapr) in enterococci. daptomycin simulations of 4 to 12 mg/kg/day (maximum concentration of drug in serum [cmax] of 57.8, 93. ...201424957825
[epidemiological analysis for enterococci isolated in aichi prefecture].we investigated vancomycin-resistant genes for clinical isolates of 353 vancomycin-resistant enterococci in the aichi medical university hospital and 120 vancomycin-resistant enterococci from the 8 facilities in aichi prefecture between april 2008 and january 2013. we detected 8, 105, 21 and 4 strains of enterococci with vana, vanb, vanc1 and vanc2/c3, respectively. among enterococci with vancomycin-resistant genes, we detected 4, 3 and 2 enterococci of vancomycin mic level 4 microg/ml with vanb ...201424956911
photodynamic killing of enterococcus faecalis in dentinal tubules using mthpc incorporated in liposomes and invasomes.the present in vitro study investigates the antimicrobial photodynamic efficiency of the photosensitizer 5,10,15,20-tetra(m-hydroxyphenyl)chlorin (mthpc) incorporated in liposomes (lip) and highly flexible invasomes (inv) on the endodontopathogenic species enterococcus faecalis in infected dental root canals.201524950607
molecular detection of virulence factors among food and clinical enterococcus faecalis strains in south brazil.the present report aimed to perform a molecular epidemiological survey by investigating the presence of virulence factors in e. faecalis isolated from different human clinical (n = 57) and food samples (n = 55) in porto alegre, brazil, collected from 2006 to 2009. in addition, the ability to form biofilm in vitro on polystyrene and the β-haemolytic and gelatinase activities were determined. clinical strains presented a higher prevalence of aggregation substance (agg), enterococcal surface protei ...201424948952
[photoreactivation of escherichia coli and enterococcus faecalis in the secondary effluent disinfected by uv-tio2].effects of photoreactivating light intensity (0-41 microw x cm(-2)) on photoreactivation of escherichia coli (e. coli) and enterococcus faecalis (e. faecalis) in the secondary effluent after uv and uv-tio2 disinfection were investigated. the results indicated that the disinfection efficiency of uv-tio2 was much higher than that of uv disinfection. the photoreactivation rate of e. coli was much higher in uv disinfection than that in uv-tio2 disinfection. under high light intensity in uv-tio2 disi ...201424946584
poloxamer-based curcumin solid dispersions for ex tempore preparation of supersaturated solutions intended for antimicrobial photodynamic therapy.abstract phototoxic effect of curcumin supersaturated solutions toward pathogenic bacteria has already been demonstrated. however, to be useful in the clinical practice, a supersaturated solution needs to be physically and chemically stabile over the relevant time period. poloxamer-based solid dispersions (sd) intended for ex tempore preparation of a supersaturated solution were designed to simultaneously facilitate dissolution and inhibit precipitation of curcumin in vitro. due to the transform ...201424946137
characteristics of prosthetic joint infections due to enterococcus sp. and predictors of failure: a multi-national study.the objective of this study was to review the characteristics and outcome of prosthetic joint infections (pji) due to enterococcus sp. collected in 18 hospitals from six european countries. patients with a pji due to enterococcus sp. diagnosed between january 1999 and july 2012 were retrospectively reviewed. relevant information about demographics, comorbidity, clinical characteristics, microbiological data, surgical treatment and outcome was registered. univariable and multivariable analyses we ...201424943469
autoimmune neutropenia of infancy with recurrent urinary tract infections: a case report.autoimmune neutropenia of infancy is characterized by minor intercurrent infections despite severe neutropenia; severe bacterial infections are uncommon. an infant developed recurrent urinary tract infections at 9 and 11 months of age. the identified uropathogens were escherichia coli and enterococcus faecalis, respectively. empirical treatment with carbapenems, as broad-spectrum antibiotics, promptly resolved the infection without sequelae. febrile neutropenic children with cancer and autoimmun ...201524942028
the effect of experimental streptococcus infection in myocarditis on some biochemical and inflammatory markers in albino rats.myocarditis is an uncommon disease that presents with a wide range of symptoms in children and adults. it is histologically characterized by varying degrees of myocardialnecrosis, edema and cellular infiltration myocardial inflammation is a nonspecificresponse to many triggers such as bacterial infection, cardio toxic agents, ormechanical injury.201324940333
new intracanal formulations containing doxycycline or chlorhexidine against enterococcus faecalis.the present study aims to evaluate the antimicrobial effect of two new intracanal preparations against e. faecalis. thirty single-rooted human canine teeth were used. the crowns were removed and the roots were instrumented using a conventional technique. three groups of ten teeth each were infected with 108 cfu/ ml of e. faecalis for 21 days. the root canals were flled with new intracanal medications containing 3% doxycycline hydrochloride (dx) or 2% chlorhexidine digluconate (chx). ten teeth re ...201424939266
antibacterial and anti-biofilm activity of ah plus with chlorhexidine and cetrimide.the use of root canal filling materials with antibacterial activity can be considered beneficial to reduce the remaining microorganisms in the root canal system, where enterococcus faecalis is often found, and prevent recurrent infection. the aim of this study was to evaluate the antimicrobial activity and capacity for inhibiting e. faecalis biofilm formation of ah plus, alone and mixed with chlorhexidine (chx), cetrimide (ctr), and combinations of the two.201424935547
effect of irrigation with tetraclean on bacterial leakage of obturated root canals.the purpose of the study presented here was to evaluate the effect of tetraclean, hypoclean, chlor-xtra, 2% chlorhexidine and 6% sodium hypochlorite/17% edta as a final irrigant on bacterial leakage of the root canal. one hundred and fifty-five extracted human maxillary central incisors were randomly divided into five experimental groups of 25 teeth each and two control groups of 15 teeth each. the root canals in each group were irrigated with 2 ml of the relevant irrigant between each filing. t ...201424933773
antimicrobial efficiency of photodynamic therapy with different irradiation durations.this study aimed to evaluate the antimicrobial efficiency of pdt and the effect of different irradiation durations on the antimicrobial efficiency of pdt.201324932123
impact of microbial cultures on proteolysis and release of bioactive peptides in fermented milk.this study aimed at evaluating co-cultures of selected microorganisms for their proteolytic activity and capability to produce fermented milk enriched with ace-inhibitory (acei) peptides. selected yeasts (torulaspora delbruekii kl66a, galactomyces geotrichum kl20b, pichia kudriavzevii kl84a and kluyveromyces marxianus kl26a) and lactic acid bacteria strains (lactobacillus plantarum lat03, lb. plantarum klat01 and the not virulent enterococcus faecalis ke06) were screened as single cultures for t ...201424929726
what role do periodontal pathogens play in osteoarthritis and periprosthetic joint infections of the knee?through the use of polymerase chain reaction (pcr)-electron spray ionization (esi)-time of flight (tof)-mass spectrometry (ms), we identified multiple periodontal pathogens within joint tissues of individuals undergoing replacement arthroplasties of the knee. the most prevalent of the periodontal pathogens were treponema denticola and enterococcus faecalis, the latter of which is commonly associated with apical periodontitis. these findings were unique to periprosthetic joint infections (pji) of ...201424921460
quality control mic ranges used for telavancin with application of a revised clsi reference broth microdilution method.the telavancin broth microdilution susceptibility testing method was revised, which provides mic results lower than those obtained by the previous method. this study was performed to reestablish the quality control ranges for telavancin when tested against the strains (updated mic range) staphylococcus aureus atcc 29213 (0.03 to 0.12 μg/ml), enterococcus faecalis atcc 29212 (0.03 to 0.12 μg/ml), and streptococcus pneumoniae atcc 49619 (0.004 to 0.015 μg/ml).201424920777
frequency and antibiotic susceptibility patterns of urinary pathogens in male outpatients in argentina.knowledge of the etiology and antimicrobial susceptibility patterns of uropathogens is important for determining the best treatment option. this study aimed to determine the distribution and antibiotic susceptibility patterns of bacterial strains isolated from adult male outpatients.201424916867
residual antimicrobial activity of mtad(®) in human dentin after obturation with gutta-percha/ah26 and resilon/realseal se at different time intervals; an ex vivo eliminate microorganisms that are responsible for pulpal and periapical infections and to prevent reinfection of the root canal system an effective chemomechanical preparation by irrigants with sustained antimicrobial activity is beneficial. hereby, we evaluated the residual antibacterial activity of mtad after canal obturation at different time intervals.201424910674
biological neutralization and biosorption of dyes of alkaline textile industry wastewater.the present work was aimed to secure biological neutralization and biosorption of dyes of an alkaline textile industrial effluent (atie) using an alkaliphilic bacterium, enterococcus faecalis strain r-16 isolated from gujarat coast. the isolate was capable and competent to bring down the ph of atie from 12.1 to 7.0 within 2 h in the presence of carbon and nitrogen sources. carboxylic group concentration (cgc), nmr and ft-ir analysis revealed production of carboxylic acid as a result of neutraliz ...201424910184
immunomodulatory properties of cell wall extract from enterococcus faecalis cect7121.the aim of this study was to investigate the immunomodulatory properties of cell wall extract from enterococcus faecalis cect7121, measuring the induction of cytokines tnf-α, il-6, il-10 and il-12 from human peripheral blood mononuclear cells (pbmcs). cell wall extract was prepared from their growth in brain heart infusion broth (18h, 35°c). subsequently, toxicity of the obtained cell wall extract was tested in balb-c mice. pbmcs were isolated from buffy coats at the blood transfusion service of ...201424907474
commensal bacteria drive endogenous transformation and tumour stem cell marker expression through a bystander effect.commensal bacteria and innate immunity play a major role in the development of colorectal cancer (crc). we propose that selected commensals polarise colon macrophages to produce endogenous mutagens that initiate chromosomal instability (cin), lead to expression of progenitor and tumour stem cell markers, and drive crc through a bystander effect.201524906974
[submucosal bacterial abscesses of the ascending colon and liver associated with portal and superior mesenteric vein thrombosis due to enterococcus faecalis infection: a case report].a 72-year-old woman with diabetes mellitus was admitted with fever and general fatigue. blood biochemistry showed elevated hepatic and biliary enzyme levels, abdominal computed tomography showed multiple liver abscesses with portal and superior mesenteric vein thrombosis, and total colonoscopy revealed a submucosal bacterial abscess in the ascending colon. the abscesses were determined to be associated with enterococcus faecalis infection. the patient was treated conservatively with antibiotics ...201424898493
modified lactic acid bacteria detect and inhibit multiresistant enterococci.we designed lactococcus lactis to detect enterococcus faecalis. upon detection, l. lactis produce and secrete antienterococcal peptides. the peptides inhibit enterococcal growth and reduce viability of enterococci in the vicinity of l. lactis. the enterococcal sex pheromone ccf10 serves as the signal for detection. expression vectors derived from pcf10, a ccf10-responsive e. faecalis sex-pheromone conjugative plasmid, were engineered in l. lactis for the detection system. recombinant host strain ...201524896372
bactericidal effect of hydroxyl radicals generated from a low concentration hydrogen peroxide with ultrasound in endodontic approach to enhance the disinfection of root canals in endodontic treatment is ultrasonic irrigation with sodium hypochlorite. reactive oxygen species, such as hydroxyl radical, are generated by biological defense systems to kill invading bacteria. ultrasonic irrigation with hydrogen peroxide may be a promising option to increase hydroxyl radical generation. we examined the bactericidal effects of hydroxyl radical generated from low concentration hydrogen peroxide with ultrasound in vitro. a ...201424895478
chemical composition of enterococcus faecalis in biofilm cells initiated from different physiologic states.enterococcus faecalis is a ubiquitous bacterium of the gut that is observed in persistent periradicular infections. its pathogenicity is associated with biofilm formation and the ability to survive under nutrient-poor (starvation) conditions. however, characteristics of chemical composition of biofilm cells developed by starved e. faecalis cells remain poorly understood. in this study, e. faecalis cells in exponential, stationary, and starvation phases were prepared and separately cultured to fo ...201424895050
production of lactic acid using a new homofermentative enterococcus faecalis isolate.lactic acid is an intermediate-volume specialty chemical for a wide range of food and industrial applications such as pharmaceuticals, cosmetics and chemical syntheses. although lactic acid production has been well documented, improved production parameters that lead to reduced production costs are always of interest in industrial developments. in this study, we describe the production of lactic acid at high concentration, yield and volumetric productivity utilizing a novel homofermentative, fac ...201524894833
in vitro synergy of ampicillin with gentamicin, ceftriaxone and ciprofloxacin against enterococcus faecalis. 201424888868
characterisation of the antibacterial effect of polyethyleneimine nanoparticles in relation to particle distribution in resin characterise the antibacterial effect of resin composite incorporating cross-linked quaternised polyethyleneimine (qpei) nanoparticles in relation to their distribution in the bulk material.201524881908
linezolid update: stable in vitro activity following more than a decade of clinical use and summary of associated resistance mechanisms.linezolid, approved for clinical use since 2000, has become an important addition to the anti-gram-positive infection armamentarium. this oxazolidinone drug has in vitro and in vivo activity against essentially all gram-positive organisms, including methicillin-resistant staphylococcus aureus (mrsa) and vancomycin-resistant enterococci (vre). the in vitro activity of linezolid was well documented prior to its clinical application, and several ongoing surveillance studies demonstrated consistent ...201424880801
inhibition of enterococcus faecalis biofilm formation by highly active lactones and lactams analogues of β-aryl substituted γ-alkylidene-γ-lactones analogues of rubrolides were synthesized from mucobromic acid and converted through a lactamization with isobutylamine into their corresponding γ-hydroxy-γ-lactams (76-85%). these lactams were converted into (z)- and (e)-γ-alkylidene-γ-lactams (23-45%). all compounds were fully characterized by ir, nmr ((1)h and (13)c), cosy and hetcor bidimensional experiments, and noe difference spectroscopy experiments when necessary. evaluation of these three ...201424880232
genetic and biochemical evidence that recombinant enterococcus spp. strains expressing gelatinase (gele) produce bovine milk-derived hydrolysates with high angiotensin converting enzyme-inhibitory activity (ace-ia).in this work, genes encoding gelatinase (gele) and serine proteinase (spre), two extracellular proteases produced by enterococcus faecalis dbh18, were cloned in the protein expression vector pmg36c, containing the constitutive p32 promoter, generating the recombinant plasmids pcg, pcsp, and pcgsp encoding gele, spre, and gele-spre, respectively. transformation of noncaseinolytic e. faecalis p36, e. faecalis jh2-2, e. faecium ar24, and e. hirae ar14 strains with these plasmids permitted detection ...201424877744
evaluation of efficacy of combinations of five endodontic sealers with five antibiotics against enterococcus faecalis - an in-vitro evaluate and compare in vitro the antibacterial efficacy of five antibiotics when added individually to five endodontic sealers against enterococcus faecalis (ef).201424876708
purulent pericarditis after liver abscess: a case report.we present the case of a 49-year-old woman, with previous clinical antecedents of recent hepatic metastasis, who was admitted to the icu due to respiratory failure and hemodynamic instability. she was found to have purulent pericarditis complicated by pericardial tamponade and pleural effusion, as well as surgical site infection, which was the origin of the disease. cultures of the surgical wound and the pericardial effusion were positive for enterococcus faecalis and escherichia coli. a pericar ...201424872819
assessment of dentinal tubule invasion capacity of enterococcus faecalis under stress conditions ex investigate the dentinal tubule invasion capacity of enterococcus faecalis under alkaline and energy starvation stress conditions.201524872016
activity of daptomycin or linezolid in combination with rifampin or gentamicin against biofilm-forming enterococcus faecalis or e. faecium in an in vitro pharmacodynamic model using simulated endocardial vegetations and an in vivo survival assay using galleria mellonella larvae.enterococci are the third most frequent cause of infective endocarditis. a high-inoculum stationary-phase in vitro pharmacodynamic model with simulated endocardial vegetations was used to simulate the human pharmacokinetics of daptomycin at 6 or 10 mg/kg of body weight/day or linezolid at 600 mg every 12 h (q12h), alone or in combination with gentamicin at 1.3 mg/kg q12h or rifampin at 300 mg q8h or 900 mg q24h. biofilm-forming, vancomycin-susceptible enterococcus faecalis and vancomycin-resista ...201424867993
demography and burden of care associated with patients readmitted for urinary tract infection.urinary tract infection (uti) is one of the most prevalent admission diagnoses in hospital-based clinical practice. despite its frequency, few data are available regarding its demographics and economic implications.201524863498
eco-designed biohybrids based on liposomes, mint-nanosilver and carbon nanotubes for antioxidant and antimicrobial coating.noncovalent entities (consisting of liposomes, phyto-nanosilver and carbon nanotubes) with interesting properties were constructed by a "green" bottom-up method. phytosynthesis of silver nanoparticles using the mentha piperita extract combines the benefits of this herb with the interesting properties of silver. the obtained silver-based biohybrids showed antioxidant and antimicrobial properties that have been considerable improved in the presence of carbon nanotubes. thus the eco-designed biocon ...201424863214
extension of bactericidal effect of sodium hypochlorite into dentinal tubules.the aim of this study was to determine the extent of disinfection mediated by 2 concentrations of sodium hypochlorite for bacteria-laden dentin wall of a root canal.201424862710
comparative antibacterial efficacy of photodynamic therapy and ultrasonic irrigation against enterococcus faecalis in vitro.enterococcus faecalis poses a challenge to the efficacy of traditional root canal disinfection methods. this study was aimed to establish a synergistic root canal disinfection strategy combining ultrasonic irrigation with photodynamic therapy (pdt) together and to test its antibacterial efficacy against e. faecalis. twenty-seven bovine root canals infected with e. faecalis were randomly divided into three groups and treated with different disinfection methods as follows: ultrasonic irrigation wi ...201424861937
[clinical distribution and antimicrobial resistance analysis of 754 pathogenic bacteria in diabetic foot infection].to explore the microbiological profiles and antibiotic susceptibility patterns of organisms isolated from diabetic foot ulcers so as to provide selection rationales of antibiotics.201424854906
detection of opsonic antibodies against enterococcus faecalis cell wall carbohydrates in immune globulin preparations.three different commercially available polyvalent immune globulins (ig) were investigated for the existence of antibodies against cell wall carbohydrates of four different e. faecalis serotypes (using a cell wall carbohydrate-enzyme-linked immunosorbent assay), and whether these antibodies mediated opsonic killing (using an opsonic-killing assay). all three ig preparations contained antibodies against all four serotypes (cps-a to cps-d). however, only one of the three ig preparations showed opso ...201424854332
low-dose cisplatin administration to septic mice improves bacterial clearance and programs peritoneal macrophage polarization to m1 phenotype.sepsis is a systemic inflammatory response to infection, and early responses of macrophages are vital in controlling the infected microorganisms. we used a cecal ligation and puncture (clp) model of sepsis to determine the role of cisplatin (0.1, 0.5 and 1 mg kg(-1)) with respect to peritoneal macrophages, controlling peritoneal/blood bacterial infection, and systemic inflammation. we found that mice which received low-dose (0.1 and 0.5 mg kg(-1)) i.p. cisplatin had lower mortality rate and impr ...201424850793
formulation and characterization of lyophilized curcumin solid dispersions for antimicrobial photodynamic therapy (apdt): studies on curcumin and curcuminoids lii.bacterial resistance to antibiotics is increasing and alternative antibacterial treatments like antimicrobial photodynamic therapy (apdt) are needed. curcumin is under investigation as a potential photosensitizer in apdt.201524842546
comparative genomic and morphological analyses of listeria phages isolated from farm environments.the genus listeria is ubiquitous in the environment and includes the globally important food-borne pathogen listeria monocytogenes. while the genomic diversity of listeria has been well studied, considerably less is known about the genomic and morphological diversity of listeria bacteriophages. in this study, we sequenced and analyzed the genomes of 14 listeria phages isolated mostly from new york dairy farm environments as well as one related enterococcus faecalis phage to obtain information on ...201424837381
urinary tract infection caused by enterococcus isolates: aetiology and antimicrobial resistance patterns.the purpose of this study was to analyse the data on resistance in enterococcus faecalis and e. faecium isolated from urine samples of inpatients from january 2010 to december 2013. compared to e. faecalis, e. faecium isolates were significantly more resistant to ampicilin, nitrofurantoin, and ciprofloxacin in the antimicrobial agents evaluated. both species showed higher resistance to high-level gentamicin. the rate of vancomycin-resistant enterococci (vre) in e. faecium was higher as compared ...201524835052
enterococcus faecalis from healthy infants modulates inflammation through mapk signaling pathways.colonizing commensal bacteria after birth are required for the proper development of the gastrointestinal tract. it is believed that bacterial colonization pattern in neonatal gut affects gut barrier function and immune system maturation. studies on the development of faecal microbiota in infants showed that the neonatal gut was first colonized with enterococci followed by other microbiota such as bifidobacterium. other studies showed that babies who developed allergy were less often colonized w ...201424830946
prevalence and detection of mixed-population enterococcal bacteremia.mixed-population (heterogeneous) enterococcal bacteremia (meb) is rarely reported. based on one occasion in which vitek2 missed a vancomycin-resistant subpopulation isolated from a patient, we developed a simple method to detect this subpopulation and determined meb frequency. the four patients presented here had either enterococcus faecium or enterococcus faecalis bacteremia caused by both vancomycin-resistant enterococci (vre) and vancomycin-susceptible enterococci (vse). no prior common antib ...201424829233
structural and electronic snapshots during the transition from a cu(ii) to cu(i) metal center of a lytic polysaccharide monooxygenase by x-ray photoreduction.lytic polysaccharide monooxygenases (lpmos) are a recently discovered class of enzymes that employ a copper-mediated, oxidative mechanism to cleave glycosidic bonds. the lpmo catalytic mechanism likely requires that molecular oxygen first binds to cu(i), but the oxidation state in many reported lpmo structures is ambiguous, and the changes in the lpmo active site required to accommodate both oxidation states of copper have not been fully elucidated. here, a diffraction data collection strategy m ...201424828494
iliopsoas abscess in children: report on five patients with a literature review.we aimed in this study to present the clinical findings in children with iliopsoas abscess (ipa) and to discuss the diagnosis and treatment. the files of five patients, hospitalized between august 2011 and june 2013 and monitored with a diagnosis of ipa, were reviewed retrospectively. demographic characteristics, symptoms and signs, laboratory examinations, and diagnostic and treatment methods of the cases were evaluated. two of the cases were females and three were males, and their ages ranged ...201424827950
isolation and biological activity of compounds from garcinia preussii.plants of the genus garcinia (clusiaceae) are traditionally used to relieve stomachaches, toothaches, and as a chew stick.201424824323
genome analysis of enterococcus faecalis bacteriophage ime-ef3 harboring a putative metallo-beta-lactamase gene.lytic enterococcus faecalis bacteriophage ime-ef3 was isolated from hospital sewage, and its genome was sequenced using high-throughput sequencing. genomic analysis and electron microscopy suggested that ime-ef3 was a member of the family siphoviridae. the phage has an isometric head and a long non-contractile tail with a 41 kb linear double-stranded dna genome. the genome encodes 69 putative proteins, with 32 annotated functionally, including proteins related to phage structure, packaging, tran ...201424818695
efficacy of three irrigation agitation techniques on bacterial elimination: a microbiologic and microscopic evaluation.the aim of this study was to compare in vitro reduction of enterococcus faecalis by using three agitation methods. fifty-five mandibular premolar teeth were used. the root canals were prepared by using protaper instruments to size f4. fresh bacterial suspensions were inoculated into each canal and the teeth were incubated at 37°c under aerobic conditions for 3 weeks. the specimens in negative control group were not contaminated and the teeth in positive control receive no further instrumentation ...201424817336
infliximab therapy increases the frequency of circulating cd16(+) monocytes and modifies macrophage cytokine response to bacterial infection.crohn's disease (cd) has been correlated with altered macrophage response to microorganisms. considering the efficacy of infliximab treatment on cd remission, we investigated infliximab effects on circulating monocyte subsets and on macrophage cytokine response to bacteria. human peripheral blood monocyte-derived macrophages were obtained from cd patients, treated or not with infliximab. macrophages were infected with escherichia coli, enterococcus faecalis, mycobacterium avium subsp. paratuberc ...201424816497
investigation of mechanism and molecular epidemiology of linezolid-resistant enterococcus faecalis in china.enterococcus is a major cause of important nosocomial infections. linezolid, the first member of an entirely new class of antibiotics (oxazolidinones), is effective against serious infections caused by enterococcus. however, resistance to linezolid has been discovered throughout the world rapidly. from 2011 to 2013, nine linezolid-resistant e. faecalis isolates were collected and the possible mechanisms of linezolid resistance, including mutations in domain v of 23s rrna genes and in ribosomal p ...201424815727
incidence, risk factors, and outcomes for enterococcus spp. blood stream infections: a population-based study.enterococci are a clinically significant cause of bloodstream infections (bsi), particularly in the nosocomial setting. the purpose of this study was to characterize the incidence, risk factors for acquisition, microbiological characteristics and mortality of enterococcal bsi within the well-defined population of a large canadian health region.201424813873
role of glycolipids in the pathogenesis of enterococcus faecalis urinary tract infection.after uropathogenic escherichia coli (upec), enterococcus faecalis is the second most common pathogen causing urinary tract infections. monoglucosyl-diacylglycerol (mglcdag) and diglucosyl-diacylglycerol (dglcdag) are the main glycolipids of the e. faecalis cell membrane. examination of two mutants in genes bgsb and bgsa (both glycosyltransferases) showed that these genes are involved in cell membrane glycolipid biosynthesis, and that their inactivation leads to loss of glycolipids dglcdag (bgsa ...201424806450
comparative cell attachment, cytotoxicity and antibacterial activity of radiopaque dicalcium silicate cement and white-coloured mineral trioxide comparatively examine the cell attachment, cytotoxicity, and antibacterial activity of radiopaque dicalcium silicate cement (rdsc) and proroot white-coloured mineral trioxide aggregate (wmta).201524802368
fabrication and characterization of bioactive and antibacterial composites for dental applications.there is an increasing clinical need to design novel dental materials that combine regenerative and antibacterial properties. in this work the characterization of a recently developed sol-gel-derived bioactive glass ceramic containing silver ions (ag-bg) is presented. the microstructural characteristics, ion release profile, zeta potential value and changes in weight loss and ph value as a function of the immersion time of ag-bg in tris buffer are evaluated. ag-bg is also incorporated into natur ...201424802300
complete genome sequencing and comparative analysis of the linezolid-resistant enterococcus faecalis strain deng1.genome level analysis of bacterial strains provides information on genetic composition and resistance mechanisms to clinically relevant antibiotics. to date, whole genome characterization of linezolid-resistant enterococcus faecalis isolated in the clinic is lacking. in this study, we report the entire genome sequence, genomic characteristics and virulence factors of a pathogenic e. faecalis strain, deng1. our results showed considerable differences in genomic characteristics and virulence facto ...201424800692
colloidal gold-based immunochromatographic strip test compromising optimised combinations of anti-s. suis capsular polysaccharide polyclonal antibodies for detection of streptococcus suis.a rapid diagnosis kit that detects streptococcus suis (s. suis) antigens from urine with an immunochromatographic stripe (ics) test was developed in this study. the ics test was produced using colloidal gold coated with polyclonal antibodies (pabs) against s. suis. the pabs were developed from rabbits immunised with s. suis serotype 2 capsular polysaccharides (cps). development of the pabs was investigated to establish their binding to cps and to determine the maximum sensitivity of two combinat ...201424800681
impact of different antimicrobial therapies on clinical and fiscal outcomes of patients with bacteremia due to vancomycin-resistant enterococci.vancomycin-resistant enterococci (vre) are a growing health problem, and uncertainties exist regarding the optimal therapy for bloodstream infection due to vre. we conducted systematic comparative evaluations of the impact of different antimicrobial therapies on the outcomes of patients with bloodstream infections due to vre. a retrospective study from january 2008 to october 2010 was conducted at detroit medical center. unique patients with blood cultures due to vre were included and reviewed. ...201424798267
enterococcus faecalis bacteremia and mitral valve endocarditis under dabigatran for stroke prevention. 201424794060
antibacterial activity of kaolinite/nanotio2 composites in relation to irradiation time.the paper addresses laboratory preparation and antibacterial activity testing of kaolinite/nanotio2 composite in respect of the daylight irradiation time. kaolinite/nanotio2 composites with 20 and 40 wt% of tio2 were laboratory prepared, dried at 105 °c and calcined at 600 °c. the calcination caused transformation of kaolinite to metakaolinite and origination of the metakaolinite/nanotio2 composite. x-ray powder diffraction, raman and ftir spectroscopic methods revealed titanium dioxide only in ...201424792569
bacterial leakage in root canals filled with ah plus and dentine bonding agents.the aim of this study was to compare the efficacy of different dentine adhesives in delaying the coronal bacterial leakage of enterococcus faecalis in filled root canals. materials and methods. ninety-five lower incisors of patients >65 years of age were instrumented using the protaper system and were irrigated with 1 ml of 2.5% sodium hypochlorite (naocl) alternated with 1 ml 17% edta between each file change. final irrigation was performed with 5 ml of 17% edta and then flushed with 5 ml of di ...201424791609
diverse outcomes of photodynamic antimicrobial chemotherapy on five enterococcus faecalis the present study, the effectiveness of photodynamic antimicrobial chemotherapy (pact) was evaluated on planktonic cells and biofilms of five enterococcus faecalis clinical isolates.201424784432
study comparing the effectiveness of chlorhexidine, calcium hydroxide and linezolid based medicaments against enterococcus faecalis.this study evaluated the efficacy of 2% chlorhexidine (cx), calcium hydroxide (ch), vitapex® (vp), linezolid (lz), a combination of lz with ch (lc) against enterococcus faecalis (ef).201424783147
correlation between biofilm formation and gele, esp, and agg genes in enterococcus spp. clinical isolates. 201424782231
health evaluation and survey of zoonotic pathogens in free-ranging capybaras (hydrochoerus hydrochaeris).capybaras (hydrochoerus hydrochaeris) are the world's largest rodents and play an epidemiologic role in the transmission of zoonotic pathogens, including the causative agents of brazilian spotted fever, leptospirosis, and others. we surveyed the health of 31 free-ranging capybaras at the alberto löfgren state park, são paulo, brazil using a variety of diagnostic methods. hematology and serum chemistry were consistent with mild malnutrition and parasitism but did not indicate severe physiologic i ...201424779462
evaluation of the presence of enterococcus faecalis in root cementum: a confocal laser scanning microscope analysis.the aim of this study is to address the cause of persistent infection of root cementum by enterococcus faecalis.201424778505
evaluation of the novel combination of daptomycin plus ceftriaxone against vancomycin-resistant enterococci in an in vitro pharmacokinetic/pharmacodynamic simulated endocardial vegetation model.daptomycin has demonstrated synergy with β-lactams against enterococcus faecium and this combination has been used successfully to treat infections refractory to daptomycin. we investigated daptomycin alone and combined with ceftriaxone against vancomycin-resistant enterococci (vre) in an in vitro pharmacokinetic/pharmacodynamic simulated endocardial vegetation (sev) model.201424777900
a newly isolated probiotic enterococcus faecalis strain from vagina microbiota enhances apoptosis of human cancer cells.this study aimed to describe probiotic properties and bio-therapeutic effects of newly isolated enterococcus faecalis from the human vaginal tract.201424775273
pyruvate formate-lyase is essential for fumarate-independent anaerobic glycerol utilization in the enterococcus faecalis strain w11.although anaerobic glycerol metabolism in enterococcus faecalis requires exogenous fumarate for nadh oxidation, e. faecalis strain w11 can metabolize glycerol in the absence of oxygen without exogenous fumarate. in this study, metabolic end product analyses and reporter assays probing the expression of enzymes involved in pyruvate metabolism were performed to investigate this fumarate-independent anaerobic metabolism of glycerol in w11. under aerobic conditions, the metabolic end products of w11 ...201424769696
evaluation of antimicrobial activity of silver nanoparticles for carboxymethylcellulose film applications in food this study, silver nanoparticles were prepared and incorporated into carboxymethylcellulose films to evaluate the antimicrobial activity for food packaging applications. the techniques carried out for material characterization were: infrared spectroscopy and thermal analysis for the silver nanoparticles and films, as well as particle size distribution for the nanoparticles and water vapor permeability for the films. the antimicrobial activity of silver nanoparticles prepared by casting method ...201424758059
transcriptomic and functional analysis of nacl-induced stress in enterococcus faecalis.the robust physiology of enterococcus faecalis facilitates tolerance to various stresses. we here report the transcriptional response of e. faecalis v583 to growth in the presence of 6.5% nacl. among the early responses observed was an immediate down-regulation of mscl, accompanied by an up-regulation of genes predicted to be involved in uptake of extracellular potassium and glycine betaine. the high nacl concentration also induced expression of chaperons and cell envelope related traits, such a ...201424755907
antimicrobial and immunomodulatory activities of pr-39 derived peptides.the porcine cathelicidin pr-39 is a host defence peptide that plays a pivotal role in the innate immune defence of the pig against infections. besides direct antimicrobial activity, it is involved in immunomodulation, wound healing and several other biological processes. in this study, the antimicrobial- and immunomodulatory activity of pr-39, and n- and c-terminal derivatives of pr-39 were tested. pr-39 exhibited an unexpected broad antimicrobial spectrum including several gram positive strains ...201424755622
exploring 5-nitrofuran derivatives against nosocomial pathogens: synthesis, antimicrobial activity and chemometric analysis.the burden of nosocomial or health care-associated infection (hcai) is increasing worldwide. according to the world health organization (who), it is several fold higher in low- and middle-income countries. considering the multidrug-resistant infections, the development of new and more effective drugs is crucial. herein, two series (i and ii) of 5-nitrofuran derivatives were designed, synthesized and assayed against microorganisms, including gram-positive and -negative bacteria, and fungi. the pa ...201424751553
prevalence and antibiotic resistance of enterococcus spp. isolated from retail cheese, ready-to-eat salads, ham, and raw specimens were analyzed in order to research enterococcus spp.: 636 samples of raw meat (227 beef, 238 poultry, and 171 pork), 278 samples of cheese (110 fresh soft cheese and 168 mozzarella cheese), 214 samples of ready-to-eat salads, and 187 samples of ham. 312 strains of enterococcus spp samples were isolated, then identified and submitted to susceptibility tests against 11 antimicrobial agents. the predominant species were enterococcus faecalis in raw meat and enterococcus faecium in re ...201424750807
clonal diversity in biofilm formation by enterococcus faecalis in response to environmental stress associated with endodontic irrigants and determine whether clonal diversity within e. faecalis affects biofilm formation when exposed to antimicrobial compounds found in endodontic medicaments and irrigants.201524749689
in vitro assessment of antimicrobial activity of pothomorphe umbellata extracts against enterococcus faecalis.due to the complex anatomy of the root canal system, biomechanical preparation is not able to completely eliminate all microorganisms present in the endodontic infections, making it necessary the use of an intracanal medication.201424748302
in vitro efficacy of antibiotic beads in treating abdominal vascular graft infections.abdominal aortic vascular graft infection often involves several different organisms. antibiotic polymethyl methacrylate (pmma) beads may be effective in controlling infection after débridement, but bacterial species identification and antibiotic susceptibility are often not available at the time of operation, generating a need for a broad-spectrum drug combination for empirical use. we sought to determine an effective antibiotic in pmma beads for use in abdominal vascular graft infection.201524745942
glycopeptide use is associated with increased mortality in enterococcus faecalis bacteraemia.enterococci are an important cause of nosocomial and community-acquired infections, with bacteraemia being one of the most common presentations. although inappropriate antimicrobial therapy has been associated with poorer outcomes in enterococcus faecalis (ef) bacteraemia, the impact of antimicrobial choice, namely β-lactam versus glycopeptide therapy, has not been well described. we sought to determine whether choice of antibiotic affects patient outcomes in ef bacteraemia.201424744303
passive ultrasonic irrigation in the presence of a low concentration of hydrogen peroxide enhances hydroxyl radical generation and bactericidal effect against enterococcus faecalis.chemomechanical procedures can be used to eliminate bacteria from root canals. however, detectable bacteria sometimes remain because of the complexity of the root canal system. endodontic passive ultrasonic irrigation (pui) with hydrogen peroxide (h2o2) may be a promising option for increasing bactericidal hydroxyl radical (ho•) generation. in this in vitro experiment, we examined the effects of ho• generated using pui and a low concentration of h2o2. an ultrasonic tip was submerged in 0.45 mol/ ...201424739706
comparison of the antibacterial effect of sodium hypochlorite and aloe vera solutions as root canal irrigants in human extracted teeth contaminated with enterococcus faecalis.the main purpose of a root canal treatment is to eliminate the bacteria and their products from the pulp space. sodium hypochlorite has excellent antibacterial properties, but also some negative features.201424738089
an in vitro comparison of furcal perforation repaired with pro-root mta and new endodontic cement in primary molar teeth- a microleakage of the most challenging procedural accidents during pulpotomy of primary molars is furcal perforation. to prevent bacterial invasion, the perforation site should be sealed as soon as possible.201424738087
occurrence of enterococcus species with virulence markers in an urban flow-influenced tropical recreational beach.median enterococci counts of beach water samples gradually increased at statistically significant levels (χ2: 26.53, df: 4; p<0.0001) with increasing proximity to river influx. the difference in proportion of antibiotic resistant enterococci in beach water and river water samples was statistically significant (p<0.05) for the tested antibiotics with river isolates generally presenting higher resistance frequencies. virulence genes cyl, esp, gele and asa were detected at varying frequencies (7.32 ...201424725825
effects of resveratrol on gut microbiota and fat storage in a mouse model with high-fat-induced obesity.recent studies have investigated the anti-obesity effect of resveratrol, but the pathways through which resveratrol resists obesity are not clear. in the present study, we hypothesize that resveratrol exerts anti-obesity effects that are likely mediated by mechanisms of regulating gut microbes, and in turn, improving fat storage and metabolism. gut microbes, glucose and lipid metabolism in high-fat diet (hf) mice in vivo are investigated after resveratrol treatment. several biochemical markers a ...201424722352
structural elucidation of srta enzyme in enterococcus faecalis: an emphasis on screening of potential inhibitors against the biofilm formation.enterococcus faecalis is a pathogenic gram-positive bacterium, which mainly infects humans through urinary tract infections. srta is an essential enzyme for survival of e. faecalis, and inhibition of this particular enzyme will reduce the virulence of biofilm formation. it is proved to be associated with the microbial surface protein embedded signal transduction mechanism and promising as a suitable anti-microbial drug target for e. faecalis. the present work gives an inclusive description of sr ...201424718729
enhanced removal of enterococcus faecalis biofilms in the root canal using sodium hypochlorite plus photon-induced photoacoustic streaming: an in vitro study.the purpose of this study was to determine the effectiveness of laser-activated irrigation by photon-induced photoacoustic streaming (pips) using er:yag laser energy in decontaminating heavily colonized root canal systems in vitro.201424717113
impact of bacterial infections on aging and cancer: impairment of dna repair and mitochondrial function of host cells.the commensal floras that inhabit the gastrointestinal tract play critical roles in immune responses, energy metabolism, and even cancer prevention. pathogenic and out of place commensal bacteria, can however have detrimental effects on the host, by introducing genomic instability and mitochondrial dysfunction, which are hallmarks of both aging and cancer. helicobacter pylori and enterococcus faecalis are bacteria of the gastrointestinal tract that have been demonstrated to affect these two hall ...201424704713
Displaying items 13101 - 13200 of 14299