Publications

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fever and prodromal infections in anti-glomerular basement membrane disease.anti-glomerular basement membrane (gbm) disease is an autoimmune disorder with rapidly progressive glomerulonephritis and alveolar hemorrhage. fever symptom and prodromal infections have been reported in many cases, but still not been elucidated.201728261931
a study of pre-operative presence of micro-organisms in affected knee joints of rheumatoid arthritis patients who need total knee arthroplasty.to evaluate, by way of intraoperative tissue culture and pathological study, the pre-operative presence of micro-organisms in knee joints of patients with rheumatoid arthritis (ra) who need total knee arthroplasty (tka).201727919672
in vitro activity of a polyhexanide-betaine solution against high-risk clones of multidrug-resistant nosocomial pathogens.to determine the in vitro activity of a polyhexanide-betaine solution against collection strains and multidrug-resistant (mdr) nosocomial isolates, including high-risk clones.201727004429
the influence of the autochthonous wastewater microbiota and gene host on the fate of invasive antibiotic resistance genes.the aim of this study was to assess the fate of invasive antibiotic resistance genes (arg) discharged in wastewater. with this objective, antibiotic resistant bacteria (arb) known to harbor specific arg were inoculated in wastewater (hospital effluent, or municipal raw and treated wastewater) and in ultra-pure sterile water microcosms. two sets of wastewater arb isolates were used - set 1, enterococcus faecalis, acinetobacter johnsonii, klebsiella pneumoniae and set 2, enterococcus faecium, acin ...201727697350
the evaluation of cultivable microbiota profile in patients with secondary endodontic infection before and after photo-activated disinfection.secondary/persistent endodontic infection can be the outcome of failure of endodontic treatment. photo-activated disinfection (pad) can be a useful adjunct to mechanical and antimicrobial agents in eliminating endopathogenic microorganisms. in this study, we evaluated the effect of pad on diversity and count of microbiota related to secondary/persistent endodontic infections.201728249746
novel bioactive root canal sealer to inhibit endodontic multispecies biofilms with remineralizing calcium phosphate ions.the objectives of this study were to: (1) develop a bioactive endodontic sealer via dimethylaminohexadecyl methacrylate (dmahdm), 2-methacryloyloxyethyl phosphorylcholine (mpc) and nanoparticles of amorphous calcium phosphate (nacp) for the first time; and (2) evaluate inhibition of early-stage and mature multispecies endodontic biofilm, bond strength to root canal dentine, and calcium (ca) and phosphate (p) ion release.201728223198
the use of bacteriophages to biocontrol oral biofilms.infections induced by oral biofilms include caries, as well as periodontal, and peri-implant disease, and may influence quality of life, systemic health, and expenditure. as bacterial biofilms are highly resistant and resilient to conventional antibacterial therapy, it has been difficult to combat these infections. an innovative alternative to the biocontrol of oral biofilms could be to use bacteriophages or phages, the viruses of bacteria, which are specific, non-toxic, self-proliferating, and ...201728108235
analysis of the expression of nlrp3 and aim2 in periapical lesions with apical periodontitis and microbial analysis outside the apical segment of teeth.to detect the distribution and expression levels of the nucleotide-binding oligomerization domain-like receptor protein 3 (nlrp3) and the absent in melanoma 2 (aim2) inflammasomes in periapical lesions and to analyse the possible microbial stimuli outside of teeth.201728193569
antibacterial activity of tribulus terrestris and its synergistic effect with capsella bursa-pastoris and glycyrrhiza glabra against oral pathogens: an in-vitro study.in this study, antimicrobial activities of an ethanol extract of tribulus terrestris aloneand in combination with capsella bursa-pastoris and glycyrrhiza glabra were examined in vitro against six pathogens namely streptococcus mutans, streptococcus sanguis, actinomyces viscosus, enterococcus faecalis staphylococcus aureus, and escherichia coli.201726101754
evaluating the effect of peptoid lipophilicity on antimicrobial potency, cytotoxicity, and combinatorial library design.growing prevalence of antibiotic resistant bacterial infections necessitates novel antimicrobials, which could be rapidly identified from combinatorial libraries. we report the use of the peptoid library agar diffusion (plad) assay to screen peptoid libraries against the eskape pathogens, including the optimization of assay conditions for each pathogen. work presented here focuses on the tailoring of combinatorial peptoid library design through a detailed study of how peptoid lipophilicity relat ...201728291947
effects of synbiotics on immunity and disease resistance of narrow-clawed crayfish, astacus leptodactylus leptodactylus (eschscholtz, 1823).the aim of this study was to evaluate the effects of prebiotics (mannanoligosaccharide and xylooligosaccharide), probiotics (enterococcus faecalis and pediococcus acidilactici) and synbiotics for 126 days on the immune responses, hemolymph indices, antioxidant enzymes, and biological responses after a 48-hour aeromonas hydrophila exposure of sub-adult crayfish (11.45 ± 1.87 g). most antibacterial activities were observed in the shell mucus of crayfish fed a diet containing xylooligosaccharide +  ...201728363588
antimicrobial assay and genetic screening of selected freshwater cyanobacteria and identification of a biomolecule dihydro-2h-pyran-2-one derivative.explorations of freshwater cyanobacteria as antimicrobial (bacteria, fungi and methicillin-resistant staphylococcus aureus (mrsa) strains) drug resource using bioassay, nrps (non-ribosomal polypeptide synthetase) and pks (polyketide synthase) genes, as well as in silico approach.201728004519
molecular viability testing of uv-inactivated bacteria.the polymerase chain reaction (pcr) is effective at detecting bacterial dna in samples, but it is unable to differentiate viable bacteria from inactivated cells or free dna fragments. new pcr-based analytical strategies have been developed to address this limitation. molecular viability testing (mvt) correlates bacterial viability with the ability to rapidly synthesize species-specific ribosomal rna precursor (pre-rrna) in response to brief nutritional stimulation. previous studies demonstrated ...201728283525
epidihydropinidine, the main piperidine alkaloid compound of norway spruce (picea abies) shows promising antibacterial and anti-candida activity.this study reports for the first time promising antibacterial and antifungal effects of epidihydropinidine, the major piperidine alkaloid in the needles and bark of norway spruce, picea abies (l.) karsten. epidihydropinidine was growth inhibitory against all bacterial and fungal strains used in our investigation, showing the lowest mic value of 5.37μg/ml against pseudomonas aeruginosa, enterococcus faecalis, candida glabrata and c. albicans. epidihydropinidine was nearly three times more active ...201728163074
antibacterial, antioxidant and anti-proliferative properties and zinc content of five south portugal herbs.crataegus monogyna l. (rosaceae) (cm), equisetum telmateia l. (equisataceae) (et), geranium purpureum vil. (geraniaceae) (gp), mentha suaveolens ehrh. (lamiaceae) (ms), and lavandula stoechas l. spp. luisieri (lamiaceae) (ls) are all medicinal.201727925492
identification of the lyso-form n-acyl intramolecular transferase in low-gc firmicutes.bacterial lipoproteins are embedded in the cell membrane of both gram-positive and gram-negative bacteria where they serve numerous functions central to cell envelope physiology. lipoproteins are tethered to the membrane by an n-acyl-s-(mono/di)-acyl-glyceryl-cysteine anchor that is variably acylated depending on genera. in several low-gc gram-positive firmicutes, a monoacyl-glyceryl-cysteine with an n-terminal fatty acid (known as the lyso form) has been reported, though how it is formed is unk ...201728320885
antimicrobial properties of black grape (vitis vinifera l.) peel extracts against antibiotic-resistant pathogenic bacteria and toxin producing molds.black grape peel possesses a substantial amount of polyphenolic antimicrobial compounds that can be used for controlling the growth of pathogenic microorganisms. the purpose of this study was to assess antibacterial and antifungal activity of black grape peel extracts against antibiotic-resistant pathogenic bacteria and toxin producing molds, respectively.201726729960
survival of vaginal microorganisms in three commercially available transport systems.transport systems are used to collect and maintain the viability of microorganisms. two amies media based transport systems, bd cultureswab™ maxv(+) amies medium without charcoal (maxv(+)) and fisherfinest(®) with amies gel transport medium without charcoal (fisherfinest(®)) were compared to a cary-blair media based transport system, starswab(®) anaerobic transport system (starswab(®)), for their capacity to maintain the viability of 17 clinical microorganisms commonly isolated from the vagina ( ...201728242337
the antibacterial activity of extracts of nine plant species with good activity against escherichia coli against five other bacteria and cytotoxicity of extracts.the development of antibiotic resistant bacteria stems from a number of factors, including inappropriate use of antibiotics in human and animal health and their prolonged use as growth promoters at sub-clinical doses in poultry and livestock production. we were interested in investigating plants that could be useful in protecting humans or animals against diarrhoea. we decided to work on extracts of nine plant species with good activity against escherichia coli based on earlier work in the phyto ...201728241818
evaluation of health potential of nutritionally enriched kodo millet (eleusine coracana) grown in himachal pradesh, india.in this study, kodo millet grains were phytochemically investigated for their nutritional and antioxidant potential for their use as functional foods. methanolic extracts of grains showed higher phenolic content and antioxidant activity. tlc studies of the extracted polyphenols from kodo millet showed the predominant presence of ferulic acid and cinnamic acid in the millet. further quantification of these polyphenols was done by using hplc, analysing ferulic acid and cinnamic acid. antagonistic ...201727507461
hydrocarbon-stapled lipopeptides exhibit selective antimicrobial activity.antimicrobial peptides (amps) occur widely in nature and have been studied for their therapeutic potential. amps are of interest due to the large number of possible chemical structural combinations using natural and unnatural amino acids, with varying effects on their biological activities. using physicochemical properties from known naturally occurring amphipathic cationic amps, several hydrocarbon-stapled lipopeptides (hslps) were designed, synthesized, and tested for antimicrobial properties. ...201728073163
cationic net charge and counter ion type as antimicrobial activity determinant factors of short lipopeptides.to get a better insight into the antimicrobial potency of short cationic lipopeptides, 35 new entities were synthesized using solid phase peptide strategy. all newly obtained lipopeptides were designed to be positively charged from +1 to +4. this was achieved by introducing basic amino acid - lysine - into the lipopeptide structure and had a hydrophobic fatty acid chain attached. lipopeptides were subjected to microbiological tests using reference strains of gram-negative bacteria: escherichia c ...201728203232
abietane diterpenes from the cones of abies numidica de lannoy ex carrière (pinaceae) and in vitro evaluation of their antimicrobial properties.eight known abietane diterpenes (1-8) were isolated for the first time from abies numidica cones (pinaceae). the structures of all compounds were established by means of 1d and 2d nmr, and uv spectral analyses. the hydromethanolic extract of a. numidica cones was tested for its antimicrobial activity against 17 gram-positive and gram-negative bacteria and against five yeasts by the use of liquid and solid medium and bioautography methods. the best antimicrobial activity was found against gram-po ...201727237830
combined chemical and physical transformation method with rbcl and sepiolite for the transformation of various bacterial species.dna transformation that delivers plasmid dnas into bacterial cells is fundamental in genetic manipulation to engineer and study bacteria. developed transformation methods to date are optimized to specific bacterial species for high efficiency. thus, there is always a demand for simple and species-independent transformation methods. we herein describe the development of a chemico-physical transformation method that combines a rubidium chloride (rbcl)-based chemical method and sepiolite-based phys ...201728185866
functional studies of e. faecalis rnase j2 and its role in virulence and fitness.post-transcriptional control provides bacterial pathogens a method by which they can rapidly adapt to environmental change. dual exo- and endonucleolytic activities of rnase j enzymes contribute to gram-positive rna processing and decay. first discovered in bacillus subtilis, rnase j1 plays a key role in mrna maturation and degradation, while the function of the paralogue rnase j2 is largely unknown. previously, we discovered that deletion of the enterococcus faecalis rnjb gene significantly att ...201728384222
d-amino acids reduce enterococcus faecalis biofilms in vitro and in the presence of antimicrobials used for root canal treatment.enterococcus faecalis is the most frequent species present in post-treatment disease and plays a significant role in persistent periapical infections following root canal treatment. its ability to persist in stressful environments is inter alia, due to its ability to form biofilms. the presence of certain d-amino acids (daas) has previously been shown to reduce formation of bacillus subtilis biofilms. the aims of this investigation were to determine if daas disrupt biofilms in early and late gro ...201728151960
a critical role for extracellular dna in dental plaque formation.extracellular dna (edna) has been identified in the matrix of many different monospecies biofilms in vitro, including some of those produced by oral bacteria. in many cases, edna stabilizes the structure of monospecies biofilms. here, the authors aimed to determine whether edna is an important component of natural, mixed-species oral biofilms, such as plaque on natural teeth or dental implants. to visualize edna in oral biofilms, approaches for fluorescently stained edna with either anti-dna ant ...201727770039
purification, characterization and functional analysis of the immune molecule lectin from the haemolymph of blue swimmer crab portunus pelagicus and their antibiofilm properties.the present study reveals purification and characterization of immune molecule lectin from the haemolymph of blue swimmer crab portunus pelagicus (pp-lec). the pp-lec was purified by affinity chromatography with mannose coupled sepharose cl-4b column and it exhibits single band with a molecular weight of 155 kda in sds-page. the surface morphology of purified pp-lec displays the homogeneous nature of protein. a distinct peak with a retention time of 3.3 min was appeared in high performance liqui ...201728110033
procalcitonin levels in bloodstream infections caused by different sources and species of bacteria.the aim of this study was to evaluate procalcitonin (pct) diagnostic accuracy in discriminating gram-negative (gn) from gram-positive (gp) bloodstream infections and determining the relationship between pct levels, infection sites, and pathogen types.201727979420
branimycins b and c, antibiotics produced by the abyssal actinobacterium pseudonocardia carboxydivorans m-227.two new antibiotics, branimycins b (2) and c (3), were produced by fermentation of the abyssal actinobacterium pseudonocardia carboxydivorans m-227, isolated from deep seawater of the avilés submarine canyon. their structures were elucidated by hrms and nmr analyses. these compounds exhibit antibacterial activities against a panel of gram-positive bacteria, including corynebacterium urealyticum, clostridium perfringens, and micrococcus luteus, and against the gram-negative bacterium neisseria me ...201728169531
[antibacterial and antifungal activity of salvia apiana against clinically important microorganisms].due to the great global concern regarding bacterial resistance to antibiotics, an ongoing search for new molecules having antibacterial activity is necessary. this study evaluated the antibacterial and anticandidal effects of a hexane extract from the root of salvia apiana. salvia extracts at concentrations of 27, 13.5, 6.8 and 3.4mg/ml caused growth inhibition of staphylococcus aureus, streptococcus pyogenes, enterococcus faecalis and candida albicans. however, no significant effect was observe ...201727595508
antimicrobial activity of aqueous and organic extracts of a saudi medicinal plant: rumex nervosus.the antimicrobial effect of aerial part of rumex nervosus obtained from the southern region of saudi arabia was evaluated on bacterial strains staphylococcus aureus, methicillin resistant s. aureus, enterococcus faecalis, escherichia coli, pseudomonas aeruginosa, and fungal strain candida albicans.201726681888
influence of sucrose on growth and sensitivity of candida albicans alone and in combination with enterococcus faecalis and streptococcus mutans to photodynamic therapy.this study has evaluated the effects of photodynamic inactivation (pdi) using erythrosine as photosensitizer and green light-emitting diode (led) on biofilms of candida albicans alone and in combination with enterococcus faecalis and streptococcus mutans. we have also evaluated the effect of sucrose on biofilm formation and bacterial growth and sensitivity to pdi. biofilms were formed in suspension of 10(6) cells/ml on plates before being grown in broth culture with and without sucrose and incub ...201728389898
evaluation of the role of probiotics in endodontic treatment: a preliminary study.the principal goal of endodontics is the prevention of periapical infection. acute and chronic apical periodontitis occur due to the persistence of pathogenic microorganisms such as enterococcus faecalis and candida albicans harboring the root canal systems of the teeth. the concept of the use of probiotics in addressing endodontic disease is new and has not been studied adequately. on the basis of the success of probiotics in periodontal treatment, this preliminary work was performed (a) to eva ...201728316949
evaluation of antimicrobial and antifungal efficacy of chitosan as endodontic irrigant against enterococcus faecalis and candida albicans biofilm formed on tooth substrate.bacterial biofilms formed on the root canal wall are often difficult to remove. this study aimed to evaluate the cytotoxic effect and antibacterial efficacy of chitosan when used as root canal irrigant against e. faecalis and candida albicans biofilm formed on tooth substrate.201728298975
additive and reducing effects between calcium hydroxide and current irrigation solutions.microorganisms should be considered to have the major role in starting and perpetuation of pulpo-periapical diseases. using intracanal medicaments is necessary to gain a bacteria-free environment in the canal system. calcium hydroxide (abbreviated as ca(oh)2), which is the most commonly used medicament in endodontic therapy, has been shown to be effective against primary sources of infection; however, its effectiveness against some microorganisms, such as candida albicans and enterococcus faecal ...201728258273
antimicrobial efficacy of probiotic-containing toothpastes: an in vitro evaluation.aim to evaluate, in vitro antimicrobial ability of two probiotic toothpastes (one containing lactobacillus paracasei, other containing lactobacillus acidophilus) and one toothpaste without probiotic separately, and in a combination with two different mouthrinses (one containing essential oils and the other containing hexitidine). methods antimicrobial susceptibility was checked by using the ditch method and clinical laboratory standard institute (clsi) guidelines. two different toothpastes with ...201728165440
biological activities of rosmarinus officinalis l. (rosemary) extract as analyzed in microorganisms and cells.r. officinalis l. is an aromatic plant commonly used as condiment and for medicinal purposes. biological activities of its extract were evaluated in this study, as antimicrobial effect on mono- and polymicrobial biofilms, cytotoxicity, anti-inflammatory capacity, and genotoxicity. monomicrobial biofilms of candida albicans, staphylococcus aureus, enterococcus faecalis, streptococcus mutans and pseudomonas aeruginosa and polymicrobial biofilms composed of c. albicans with each bacterium were form ...201728093936
in vitro antimicrobial and cytotoxic effects of anacardium occidentale and mangifera indica in oral care.oral health is an integral and important component of general health. infectious diseases such as caries, periodontal, and gingivitis indicate the onset of imbalance in homeostasis between oral micro biota and host. the present day medicaments used in oral health care have numerous side effects. the uses of herbal plants as an alternative have gained popularity due to side effects of antibiotics and emergence of multidrug resistant strains. anacardium occidentale (cashew) and mangifera indica (m ...201725709341
antibacterial efficacy of azadirachta indica, mimusops elengi and 2% chx on multispecies dentinal biofilm.to check the antimicrobial activity of azadirachta indica (neem), mimusops elengi (bakul), and chlorhexidine gluconate (chx) on multispecies biofilm of common endodontic pathogens such as streptococcus mutans, enterococcus faecalis, staphylococcus aureus and candida albicans.201726752840
evaluation of antibacterial and antifungal activity of new calcium-based cement (biodentine) compared to mta and glass ionomer cement.to evaluate the antibacterial and antifungal properties of calcium-based cement, biodentine (ca3sio2), compared to commercial glass ionomer cements (gics) and mineral trioxide aggregate (mta).201725657526
antimicrobial activity of aspilia latissima (asteraceae).we evaluated the antimicrobial activity of aspilia latissima - an abundant plant from the brazilian pantanal region - against candida albicans, candida parapsilosis, candida krusei, candida tropicalis, pseudomonas aeruginosa, enterococcus faecalis, escherichia coli and staphylococcus aureus. the crude extracts and fractions showed activity in all tested microorganisms. the chloroform fraction of the leaves and roots showed the most antimicrobial activity against s. aureus, with an mic of 500 μg/ ...201726691468
evaluation of minimal inhibitory concentration of two new materials using tube dilution method: an in vitro study.the aim of the study is to evaluate and compare the antimicrobial efficacy of two new materials mta plus and biodentine with proroot mta using tube dilution method.201725829698
comparative evaluation of antimicrobial efficacy of qmix™ 2 in 1, sodium hypochlorite, and chlorhexidine against enterococcus faecalis and candida albicans.the aim of this study is to compare the antimicrobial efficacy of qmix™ 2 in 1, sodium hypochlorite (naocl), and chlorhexidine (chx) against enterococcus faecalis and candida albicans.201725829691
microbiologic evaluation of matricaria and chlorhexidine against e. faecalis and c. albicans.to evaluate the antimicrobial activity of different concentrations of matricaria chamomilla and chlorhexidine gel against candida albicans and enterococcus faecalis.201726097333
in vitro antimicrobial activity of auxiliary chemical substances and natural extracts on candida albicans and enterococcus faecalis in root canals.the aim of this study was to evaluate the antimicrobial activity of auxiliary chemical substances and natural extracts on candida albicans and enterococcus faecalis inoculated in root canals.201723739849
effect of zingiber officinale and propolis on microorganisms and endotoxins in root canals.the purpose of this study was to evaluate the effectiveness of glycolic propolis (pro) and ginger (gin) extracts, calcium hydroxide (ch), chlorhexidine (clx) gel and their combinations as icms (icms) against candida albicans, enterococcus faecalis, escherichia coli and endotoxins in root canals.201723559108
prevalence of multidrug resistant uropathogenic bacteria in pediatric patients of a tertiary care hospital in eastern india.today, because systemic infections such as urinary tract infection (uti) affect even pediatric patients, antibiotic resistant bacteria have become a constant clinical challenge. in the present study, a total of 1054 urine samples were collected from pediatric patients over 18 months. from these samples, 510 isolates of pathogenic bacteria were collected using hicrome uti agar. antibiotic sensitivity tests of isolates were performed using the kirby-bauer method. two gram-positive bacteria (entero ...201726617250
postmortem in vitro ethanol production-it could be more common than we think!the blood alcohol concentration (bac) is the most frequent determination in a forensic toxicology laboratory. despite its apparent simplicity, the results interpretation can be complex and always have relevant social and legal implications, particularly in postmortem analysis. in the present report we describe the case of a 55-year-old male with an apparent natural death by myocardial infarction, whose initial bac was 0.18g/l but, in repeated determinations prompted by discrepancies observed in ...201728108144
searching for a potential antibacterial lead structure against bacterial biofilms among new naphthoquinone compounds.the aims of this study were to design, synthesize and to evaluate 2-hydroxy-3-phenylsulfanylmethyl-[1,4]-naphthoquinones against gram-negative and gram-positive bacterial strains, including methicillin-resistant staphylococcus aureus (mrsa) and its biofilm, to probe for potential lead structures.201727930849
the safe enterocin dd14 is a leaderless two-peptide bacteriocin with anti-clostridium perfringens activity.enterococcus faecalis 14, a strain previously isolated from meconium, displayed activity against four clostridium perfringens isolates when co-cultured on agar plates. the anti-clostridium activity was ascribed to the production of enterocin dd14, which was subsequently purified. the minimum inhibitory concentration (mic) of enterocin dd14 against one collection strain and one clinical c. perfringens strain was determined at 50 µg/ml. furthermore, using the intestinal epithelial cell line ipec-1 ...201728104423
enterocin b3a-b3b produced by lab collected from infant faeces: potential utilization in the food industry for listeria monocytogenes biofilm management.enterococcus faecalis b3a-b3b produces the bacteriocin b3a-b3b with activity against listeria monocytogenes, staphylococcus aureus, methicillin-resistant staphylococcus aureus (mrsa) and clostridium perfringens, but apparently not against fungi or gram-negative bacteria, except for salmonella newport. b3a-b3b enterocin has two different nucleotides but similar amino acid composition to the class iib mr10a-mr10b enterocin. b3a-b3b consists of two peptides of predicted molecular mass of 5176.31 da ...201727878401
antimicrobial resistance and virulence profile of enterococci isolated from poultry and cattle sources in nigeria.this study investigated the occurrence, antimicrobial resistance and virulence of enterococcus from poultry and cattle farms. three hundred and ninety samples: cloacal/rectal swabs (n = 260) and manure (n = 130] were processed for recovery of enterococcus species. standard bacteriological methods were used to isolate, identify and characterize enterococcus species for antimicrobial susceptibility and expression of virulence traits. detection of antibiotic resistance and virulence genes was carri ...201727987112
prenylated flavonoids from the stems and roots of tripterygium wilfordii.phytochemical investigation on the stems and roots of tripterygium wilfordii led to the isolation and characterization of three new prenylated flavanones, tripteryols a-c (1-3), along with (±)-5,4'-dihydroxy-2'-methoxy-6',6″-dimethypyraro-(2″,3″:7,8)-6-methyflavanone (4), and ((2s)-5,7,4'-trihydroxy-2'-methoxy-8,5'-di(3-methyl-2-butenyl)-6-methylflavanone (5). structures of the compounds 1-5 were elucidated using spectroscopic techniques, such as uv, ir, nmr (1d and 2d), and hresi-ms. tripteryol ...201728389278
comparative genomics of enterococcus spp. isolated from bovine feces.enterococcus is ubiquitous in nature and is a commensal of both the bovine and human gastrointestinal (gi) tract. it is also associated with clinical infections in humans. subtherapeutic administration of antibiotics to cattle selects for antibiotic resistant enterococci in the bovine gi tract. antibiotic resistance genes (args) may be present in enterococci following antibiotic use in cattle. if located on mobile genetic elements (mges) their dissemination between enterococcus species and to pa ...201728270110
n-acetylcysteine inhibits growth, adhesion and biofilm formation of gram-positive skin pathogens.n-acetylcysteine (nac) is a non-antibiotic drug with antimicrobial properties against biofilm phenotypes of gram-positive and gram-negative bacteria. our aim was to assess the effects of nac on the growth of gram-positive human skin and mucous membrane pathogens in the planktonic and biofilm phases. the minimum inhibitory concentrations (mics) of nac against enterococcus faecalis, corynebacterium ammoniagenes, mycobacterium smegmatis, propionibacterium acnes, staphylococcus aureus, s. epidermidi ...201728237766
evaluation of dental adhesive systems incorporating an antibacterial monomer eugenyl methacrylate (egma) for endodontic restorations.the purpose of this study was to incorporate egma, an antibacterial monomer into two commercial dental adhesive systems for their application in endodontic restoration with the aim to disinfect the root canal space before curing and to inhibit bacterial growth on their surfaces after being cured.201728245928
[profile of bacterial resistance in pediatric urinary tract infections in 2014].in pediatric units, bacteria-producing extended-spectrum-betalactamase (esbl) have an increasing prevalence among bacteria causing febrile urinary tract infections (utis). the purpose of this study was to evaluate the epidemiology of bacteria resistance patterns observed in utis, in order to assess the current antibiotic treatment protocols. this study is based upon a single-center retrospective chart review of the cytobacteriological urine cultures performed in utis between 1 january and 31 dec ...201728131557
effect of preoperative biliary drainage on bacterial flora in bile of patients with periampullary cancer.patients with obstructive jaundice due to periampullary tumours may undergo preoperative biliary drainage (pbd). the effect of pbd on the microbiome of the biliary system and on postoperative outcome remains unclear.201728121036
development of a rapid identification method for the differentiation of enterococcus species using a species-specific multiplex pcr based on comparative genomics.enterococci are lactic acid bacteria that are commonly found in food and in animal gut. since 16 s ribosomal rna (rrna) sequences, genetic markers for bacterial identification, are similar among several enterococcus species, it is very difficult to determine the correct species based on only 16 s rrna sequences. therefore, we developed a rapid method for the identification of different enterococcus species using comparative genomics. we compared 38 genomes of 13 enterococcus species retrieved fr ...201728229213
identification and prevalence of tetracycline resistance in enterococci isolated from poultry in ilishan, ogun state, nigeria.tetracycline is one of the most frequently used antibiotics in nigeria both for human and animal infections because of its cheapness and ready availability. the use of tetracycline in animal husbandry could lead to horizontal transfer of tet genes from poultry to human through the gut microbiota, especially enterococci. therefore, this study is designed to identify different enterococcal species from poultry feces in selected farms in ilishan, ogun state, nigeria, determine the prevalence of tet ...201726957873
exogenous endophthalmitis caused by enterococcus casseliflavus: a case report and discussion regarding treatment of intraocular infection with vancomycin-resistant enterococci.endophthalmitis caused by enterococci is rare, and cases involving vancomycin-resistant enterococci are even more so. due to the poor bioavailability of many antibiotics in the vitreous chamber, special considerations are required when choosing antibiotics to treat these infections. the authors report the first case of exogenous endophthalmitis caused by enterococcus casseliflavus via the unique mechanism of high-velocity water stream trauma from a toy water gun. a previously healthy four-year o ...201726744592
poly(methyl methacrylate) surface modification for surfactant-free real-time toxicity assay on droplet microfluidic platform.microfluidic droplet reactors have many potential uses, from analytical to synthesis. stable operation requires preferential wetting of the channel surface by the continuous phase which is often not fulfilled by materials commonly used for lab-on-chip devices. here we show that a silica nanoparticle (sinp) layer coated onto a poly(methyl methacrylate) (pmma) and other thermoplastics surface enhances its wetting properties by creating nanoroughness, and allows simple grafting of hydrocarbon chain ...201728375597
chitosan-propolis nanoparticle formulation demonstrates anti-bacterial activity against enterococcus faecalis biofilms.propolis obtained from bee hives is a natural substance with antimicrobial properties. it is limited by its insolubility in aqueous solutions; hence ethanol and ethyl acetate extracts of malaysian propolis were prepared. both the extracts displayed antimicrobial and anti-biofilm properties against enterococcus faecalis, a common bacterium associated with hospital-acquired infections. high performance liquid chromatography (hplc) analysis of propolis revealed the presence of flavonoids like kaemp ...201728362873
rapid molecular identification of fecal origin-colonies growing on enterococcus spp.-specific culture methods.the mei, chromocult(®) enterococci, and m-enterococcus culture-based methods used to assess water quality by the detection of enterococcus spp. were first compared in terms of sensitivity using (1) 41 different type strains of enterococcus spp. and (2) environmental colonies identified by 16s rrna sequencing. then, two specific-rtpcr assays targeting enterococcus spp. and enterococcus faecalis/faecium were tested for their ability to confirm the identity of putative enterococcal colonies. the me ...201728362305
attributable mortality of hospital-acquired bloodstream infections in ireland.to estimate the attributable mortality of hospital-acquired bloodstream infections (ha-bsi) in ireland.201728359546
prevalence and bacterial isolates of mastitis in dairy farms in selected districts of eastern harrarghe zone, eastern ethiopia.the study was conducted from november 2015 to april 2016 to estimate the prevalence of clinical and subclinical mastitis in lactating cows, to assess the associated risk factors, and to isolate the major bacterial pathogens in dairy farms in selected district of eastern harrarghe zone, eastern ethiopia. the study was carried out in 384 dairy cows based on data collection, farm visit, animal examination, california mastitis test (cmt), and isolation bacterial pathogens using standard techniques. ...201728352648
synergistic effect of haloduracin and chloramphenicol against clinically important gram-positive bacteria.the emergence of drug-resistant pathogens has triggered the search for more efficient antimicrobial agents and formulations for treatment of infections. in recent years, combination therapy has become one of the effective clinical practices in treating infections. the present study deals with the effect of haloduracin, a lantibiotic bateriocin and chloramphenicol against clinically important bacteria. the combined use of haloduracin and chloramphenicol resulted in remarkable synergy against a sp ...201728352561
good bugs vs bad bugs: evaluation of inhibitory effect of selected probiotics against enterococcus faecalis.the main goal of endodontics is the prevention of apical periodontitis. this is due to the presence of persistent pathogenic microorganisms, such as enterococcus faecalis, and its ability to directly cause acute and chronic inflammation in the periapical tissues. lactobacillus has been shown to promote health in the intestines as well as to inhibit the growth of certain problematic oral bacteria. this study explores shifting the established paradigm of endodontic treatment, which has focused on ...201728349910
a 10-year profile of enterococcal bloodstream infections at a tertiary-care hospital in japan.the first aim of this study is to characterize the epidemiology of enterococcal bloodstream infections (bsis) at a japanese tertiary-care hospital. the second aim is to identify predictive factors for 30-day mortality.201728385565
safety assessment of commensal enterococci from dogs.enterococci form a complex, diverse, and very important group of bacteria from the technological and food safety aspect, or from the health-improving aspect as probiotics. generally, enterococci are considered to be of low pathogenic potential, which is associated mostly with clinical strains. in these strains, production of virulence factors as well as resistance to many antimicrobial drugs could complicate treatment of nosocomial infections. because there is a lack of information on incidence ...201728316009
maldi-tof mass spectrometry as a useful tool for identification of enterococcus spp. from wild birds and differentiation of closely related species.the aim of this study was to explore the accuracy and feasibility of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (maldi-tof ms) in identifying bacteria from environmental sources, as compared to rpoa gene sequencing, and to evaluate the occurrence of bacteria of the genus enterococcus in wild birds. in addition, a phyloproteomic analysis of certain enterococcus species with spectral relationships was performed. the enterococci were isolated from 25 species of wil ...201728285496
antagonistic activity of lactobacillus reuteri strains on the adhesion characteristics of selected pathogens.adhesion ability of probiotics is the key factor that decides their colonization in the gastrointestinal tract and potential to inhibit pathogens. therefore, adhesion ability can be considered as a key determinant for probiotic efficacy. presents study documents the antagonistic activity of viable/untreated, lithium chloride (licl) treated or heat-killed forms of eight probiotic lactobacillus reuteri strains on the adhesion characteristics of selected pathogens. all strains investigated were abl ...201728377765
study on the excision and integration mediated by class 1 integron in enterococcus faecalis.recognized as a mobile genetic element, integron is correlated to the excision and integration of exogenous genes, especially bacterial resistance genes. however, most of the investigations focused on gram-positive bacteria with few exceptions. in this study, enterococcus faecalis was selected to investigate the excision and integration of class 1 integron. a total of eight plasmids were subjected to establish the transformants for excision and integration test. as results showed, positive excis ...201728390978
stimulatory effects of heat-killed enterococcus faecalis on cell-mediated immunity in fish.intracellular bacterial and viral diseases are widespread in the aquaculture industry and cause serious economic losses. development of effective vaccines and adjuvants that can induce cell-mediated immunity is urgently needed for prevention of these diseases. here we report the immunostimulatory effects of probiotic bacteria ''e. faecalis'' in ginbuna crucian carp carassius auratus langsdorfii. intraperitoneal injection of heat-killed e. faecalis induced an increase in cd4-1(+) lymphocytes, cd8 ...201728389237
efficacy of telavancin alone and in combination with ampicillin in a rat model of enterococcus faecalis endocarditis.we first assessed telavancin (tlv) pharmacokinetics after a single subcutaneous dose of 35 mg/kg. the pharmacokinetic data were used to predict a tlv dose that simulates human exposure and the efficacy of tlv was then evaluated using tlv 21 mg/kg q 12 h against enterococcus faecalis og1rf (tlv mic of 0.06 μg/ml) in a rat endocarditis model with an indwelling catheter. therapy was given for 3 days with tlv, daptomycin (dap) or ampicillin (amp) monotherapy and with combinations of tlv plus amp, am ...201728320712
effect of using different vehicles on the physicochemical, antimicrobial, and biological properties of white mineral trioxide aggregate.this study evaluated the physicochemical, antimicrobial, and biological properties of white mineral trioxide aggregate (mta) mixed with aqueous and propylene glycol extracts of arctium lappa l. and casearia sylvestris sw.201728320540
biofilm formation capability of enterococcal strains causing urinary tract infection vis-a-vis colonisation and correlation with enterococcal surface protein gene.data regarding differences in biofilm formation among urinary isolates of enterococci causing nosocomial infection versus asymptomatic colonisation is lacking. conflicting data are available for the role of enterococcal surface protein (esp) gene in the development of enterococcal biofilms.201728303818
draft genome sequence of a multidrug-resistant strain of enterococcus faecalis, pm01, isolated from the nest of an american bushtit, psaltriparius minimus.pathogenic microorganisms associated with avian nests may detrimentally impact parental health and nest success for the nest primary users, potentially neighboring avian or terrestrial species, including humans. here, we report the genome sequence of enterococcus faecalis strain pm01, isolated from a failed nest of american bushtits, psaltriparius minimus.201728302771
purulent pericarditis as a complication of bacteraemic enterococcus faecalis urinary tract infection.purulent pericarditis is a rare clinical entity in the modern antibiotic era. the most common portal of entry is thought to be direct extension from a primary lung source and is usually caused by staphylococcus aureus, streptococcus pneumoniae or haemophilus influenzae we report the case of a man aged 69 years who presented with purulent pericarditis due to enterococcus faecalis likely caused by haematogenous spread from a urinary tract source. urgent pericardiocentesis was vital and restored hi ...201728298381
the impact of a 940 nm diode laser with radial firing tip and bare end fiber tip on enterococcus faecalis in the root canal wall dentin of bovine teeth: an in vitro study.this in vitro study aimed to compare the bactericidal effect of two different laser delivery systems, a radial firing tip (rft) and bare end fiber tip (bft) used with the 940 nm diode laser on enterococcus faecalis inoculated onto bovine radicular dentin.201728294701
successful salvage treatment of native valve enterococcus faecalis infective endocarditis with telavancin: two case reports.infective endocarditis (ie) one-year mortality rates approach 40%. here, we report two native valve enterococcus faecalis ie cases in patients successfully treated with telavancin. an 88-year-old with mitral valve endocarditis and a penicillin allergy, initially treated with intravenous vancomycin, was switched to telavancin. a 69-year-old, who previously received amoxicillin and intravenous vancomycin for presumed enterococcal bacteraemia, was diagnosed with dual valve endocarditis for which he ...201728293958
sonication of arthroplasty implants improves accuracy of periprosthetic joint infection cultures.there is evidence that sonication of explanted prosthetic hip and knee arthroplasty components with culture of the sonication fluid may enhance diagnostic sensitivity. previous studies on the use of implant sonicate cultures have evaluated diagnostic thresholds but did not elaborate on the clinical importance of positive implant sonicate cultures in the setting of presumed aseptic revisions and did not utilize consensus statements on periprosthetic joint infection (pji) diagnosis when defining t ...201728290115
antimicrobial properties of copper nanoparticles and amino acid chelated copper nanoparticles produced by using a soya extract.this paper reports a comparison of the antibacterial properties of copper-amino acids chelates and copper nanoparticles against escherichia coli, staphylococcus aureus, and enterococcus faecalis. these copper-amino acids chelates were synthesized by using a soybean aqueous extract and copper nanoparticles were produced using as a starting material the copper-amino acids chelates species. the antibacterial activity of the samples was evaluated by using the standard microdilution method (clsi m100 ...201728286459
fosfomycin and comparator activity against select enterobacteriaceae, pseudomonas, and enterococcus urinary tract infection isolates from the united states in 2012.fosfomycin is a broad-spectrum cell wall active agent that inhibits the mura enzyme involved in peptidoglycan synthesis and is fda-approved for treatment of uncomplicated urinary tract infections (utis) caused by escherichia coli and enterococcus faecalis in women. data regarding the susceptibility of recent uti isolates to fosfomycin are limited.201728285420
detection of novel oxazolidinone and phenicol resistance gene optra in enterococcal isolates from food animals and animal carcasses.altogether 7720 enterococcus faecalis and 3939 e. faecium isolated from food animals and animal carcasses during 2003-2014 in korea were investigated to determine if linezolid-resistant (lr) enterococci (≥8μg/ml) are present. overall, 12 e. faecalis and 27 e. faecium recovered from chickens (n=32), pigs (n=6), and cattle (n=1) were resistant to linezolid and were further characterized using molecular methods most lr isolates were also resistant to chloramphenicol (97.44%) and florfenicol (92.31% ...201728284617
biological activities of undescribed north american lichen species.lichens provide a large array of compounds with the potential for pharmaceutical development. in the present study, extracts from three previously undescribed north american lichen species were examined for antioxidant, antibacterial and anticancer activities.201728369962
antimicrobial activity and chemical composition of "kpètè-kpètè": a starter of benin traditional beer tchoukoutou.the aim of this study was to investigate the antibacterial effect of the crude starter "kpètè-kpètè" and lactic acid bacteria used during the production of "tchoukoutou." to achieve this, a total of 11 lactic acid bacteria and 40 starter samples were collected from four communes. the samples were tested on 29 gram + and - strains by disk diffusion method. the minimum inhibitory and bactericidal concentrations of starter and lactic acid bacteria were determined by conventional methods. organic ac ...201728367445
vitreous hemorrhage as an early sign of acute bacterial endophthalmitis following intravitreal ranibizumab injection.to report a case of acute bacterial endophthalmitis after antivascular endothelial growth factor injection with a rare presentation of vitreous hemorrhage.201728365853
negative allosteric regulation of enterococcus faecalis small alarmone synthetase relq by single-stranded rna.the alarmone nucleotides guanosine pentaphosphate (pppgpp) and tetraphosphate (ppgpp), collectively referred to as (p)ppgpp, are key regulators of bacterial growth, stress adaptation, pathogenicity, and antibiotic tolerance. we show that the tetrameric small alarmone synthetase (sas) relq from the gram-positive pathogen enterococcus faecalis is a sequence-specific rna-binding protein. relq's enzymatic and rna binding activities are subject to intricate allosteric regulation. (p)ppgpp synthesis i ...201728320944
chemical composition, antioxidant and antibacterial activities of tamarix balansae j. gay aerial parts.a previously undescribed phenolic sulphate ester, potassium 34-dihydroxy-3-methoxybenzoic acid methyl ester-5-sulphate (1), along with nine known flavonoids, kaempferol-3-o-potassium sulphate-4',7-dimethyl ether (2), kaempferol-4',7-dimethyl ether (3), rhamnocitrin-3-o-potassium sulphate (4), rhamnocitrin (5), kaempferol (6), quercetin (7), afzelin (8), quercetin-3-o-α-l-rhamnopyranoside (9) and luteolin-3'-o-potassium sulphate (10) were isolated from the aerial parts of tamarix balansae. struct ...201728281364
requirement of the crors two-component system for resistance to cell-wall-targeting antimicrobials in enterococcus faecium.enterococci are serious opportunistic pathogens that are resistant to many cell-wall-targeting antibiotics. the crors two-component signaling system responds to antibiotic-mediated cell-wall stress and is critical for resistance to cell-wall-targeting antibiotics in enterococcus faecalis here, we identify and characterize an orthologous two-component system found in enterococcus faecium that is functionally equivalent to the crors system of e. faecalis deletion of crors in e. faecium resulted in ...201728223383
comparison of methods for identifying causative bacterial microorganisms in presumed acute endophthalmitis: conventional culture, blood culture, and pcr.identification of bacterial pathogens in endophthalmitis is important to inform antibiotic selection and treatment decisions. hemoculture bottles and polymerase chain reaction (pcr) analysis have been proposed to offer good detection sensitivity. this study compared the sensitivity and accuracy of a blood culture system, a pcr approach, and conventional culture methods for identification of causative bacteria in cases of acute endophthalmitis.201728222703
ultraviolet (uv)-reflective paint with ultraviolet germicidal irradiation (uvgi) improves decontamination of nosocomial bacteria on hospital room surfaces.an ultraviolet germicidal irradiation (uvgi) generator (the torch™, clordisys solutions, inc.) was used to compare the disinfection of surface coupons (plastic from a bedrail, stainless steel, and chrome-plated light switch cover) in a hospital room with walls coated with ultraviolet (uv)-reflective paint (lumacept) or standard paint. each surface coupon was inoculated with methicillin-resistant staphylococcus aureus (mrsa) or vancomycin-resistant enterococcus faecalis (vre), placed at 6 differe ...201728278065
effect of photodynamic therapy in combination with various irrigation protocols on an endodontic multispecies biofilm ex vivo.to analyse the antibacterial effect of photodynamic therapy (pdt) in combination with various irrigation protocols on a multispecies biofilm in root canals ex vivo.201728276583
[effects of epigallocatechin-3-gallate modification on the bonding stability of an etch-and-rinse adhesive to intraradicular dentin].objective: to evaluate the effects of epigallocatechin-3-gallate (egcg) modification on the bonding stability of an etch-and-rinse adhesive to intraradicular dentin, and to find a new strategy to improve the stability of bonding interface. methods: egcg was incorporated into single bond 2 (sb2) with the concentration of 200 mg/l and 400 mg/l respectively to fabricated experimental adhesives group a and group b, while single bond 2 without egcg was used as control group. laser scanning confocal m ...201728253588
discovery of substituted oxadiazoles as a novel scaffold for dna gyrase inhibitors.dna gyrase and topoisomerase iv are type iia topoisomerases that are essential bacterial enzymes required to oversee the topological state of dna during transcription and replication processes. their atpase domains, gyrb and pare, respectively, are recognized as viable targets for small molecule inhibitors, however, no synthetic or natural product gyrb/pare inhibitors have so far reached the clinic for use as novel antibacterial agents, except for novobiocin which was withdrawn from the market. ...201728246042
adjuvant antibiotic activity of acidic sophorolipids with potential for facilitating wound healing.the sophorolipid class of biosurfactants are finding increasing use in personal care as well as pharmaceutical products and have the potential to disrupt biofilm formation and inhibit growth in a variety of clinically relevant organisms. in order to investigate potential biomedical applications of sophorolipids derived from non-pathogenic organisms, we fractionated and purified glycolipid biosurfactant sophorolipids produced by the yeast starmerella bombicola, which yielded both acidic c 18-1 ac ...201728242666
porphyromonas gingivalis is the most abundant species detected in coronary and femoral arteries.an association between oral bacteria and atherosclerosis has been postulated. a limited number of studies have used 16s rna gene sequencing-based metagenomics approaches to identify bacteria at the species level from atherosclerotic plaques in arterial walls. the objective of this study was to establish detailed oral microbiome profiles, at both genus and species level, of clinically healthy coronary and femoral artery tissues from patients with atherosclerosis. tissue specimens were taken from ...201728326156
loop-mediated isothermal amplification label-based gold nanoparticles lateral flow biosensor for detection of enterococcus faecalis and staphylococcus aureus.the report describes a simple, rapid and sensitive assay for visual and multiplex detection of enterococcus faecalis and staphylococcus aureus based on multiple loop-mediated isothermal amplification (mlamp) and lateral flow biosensor (lfb). detection and differentiation of the ef0027 gene (e. faecalis-specific gene) and nuc gene (s. aureus-specific gene) were determined using fluorescein (fitc)-and digoxin-modified primers in the mlamp process. in the presence of biotin- and fitc-/digoxin-modif ...201728239371
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