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differentiation of streptococcus uberis from streptococcus parauberis by polymerase chain reaction and restriction fragment length polymorphism analysis of 16s ribosomal dna.streptococcus uberis type ii has been proposed recently as a separate species designated streptococcus parauberis (a. m. williams and m. d. collins, j. appl. bacteriol. 68:485-490, 1990). differentiation of s. parauberis from s. uberis has been possible only by dna-dna hybridization or 16s rrna sequencing, since the biochemical and serological characteristics of the two species are indistinguishable. a simple and reliable technique was developed for differentiating s. parauberis (s. uberis type ...19911684585
molecular taxonomic studies on streptococcus uberis types i and ii. description of streptococcus parauberis sp. nov.the nucleotide sequences of 16s ribosomal-rna of streptococcus uberis types i and ii were determined by reverse transcriptase. comparative analysis of the sequence data showed that the two types are phylogenetically distinct and worthy of separate species status. a new species streptococcus parauberis is proposed for the type ii strains. the type strain of strep. parauberis is ncdo 2020.19901695217
development and use of species-specific oligonucleotide probes for differentiation of streptococcus uberis and streptococcus parauberis.oligonucleotide probes specific for 16s rrna and capable of differentiating streptococcus uberis and s. parauberis from each other and other esculin-hydrolyzing streptococci were developed. use of a mini-rna extraction technique for gram-positive cocci associated with bovine mastitis has allowed the probes to be used for identification of esculin-hydrolyzing streptococci from two dairy herds at the institute for animal health, compton, united kingdom. one hundred seventy-nine of 206 isolates wer ...19938417033
the abp locus of streptococcus uberis encodes a protein homologous to polar amino acid and opine binding proteins of gram-negative bacteria.a gene locus abp was identified immediately upstream of the camp factor gene cfu in streptococcus uberis. an open reading frame capable of coding for a 277-residue protein was identified. on the basis of sequence characteristics, the abp gene product is potentially a polar amino acid and opine binding component of an atp-binding cassette type (abc-type) transport system similar to those of gram-negative bacteria. this membrane protein is likely lipid modified at its amino terminus and was presen ...19989830108
evaluation of pcr methods for rapid identification and differentiation of streptococcus uberis and streptococcus parauberis.streptococcus uberis and streptococcus parauberis reference strains and isolates obtained from routine diagnostics were investigated by pcr with oligonucleotide primers designed according to species-specific parts of the 16s rrna gene, the 23s rrna gene, and the 16s-23s rrna intergenic spacer region of both species. all three primer pairs allowed an identification of 67 isolates as s. uberis and 4 isolates as s. parauberis.200111283100
development of a rapid and sensitive test for identification of major pathogens in bovine mastitis by pcr.bovine mastitis is the most important source of loss for the dairy industry. a rapid and specific test for the detection of the main pathogens of bovine mastitis is not actually available. molecular probes reacting in pcr with bacterial dna from bovine milk, providing direct and rapid detection of escherichia coli, staphylococcus aureus, streptococcus agalactiae, streptococcus dysgalactiae, streptococcus parauberis, and streptococcus uberis, have been developed. two sets of specific primers were ...200111427573
dormancy as a survival strategy of the fish pathogen streptococcus parauberis in the marine environment.the fate of streptococcus parauberis in seawater and sediment microcosms at different temperatures (6 and 22 degrees c) was investigated by comparing the survival dynamics of 2 strains of this bacterial species, isolated respectively from diseased turbot and cattle. the turbot and the bovine isolate showed similar survival kinetics, remaining culturable for approximately 1 mo in water and 6 mo in sediment. a slight influence of temperature on the stability of the cells was observed, in that the ...200212542090
multiplex pcr assay for detection of bacterial pathogens associated with warm-water streptococcosis in fish.a multiplex pcr-based method was designed for the simultaneous detection of the main pathogens involved in warm-water streptococcosis in fish (streptococcus iniae, streptococcus difficilis, streptococcus parauberis, and lactococcus garvieae). each of the four pairs of oligonucleotide primers exclusively amplified the targeted gene of the specific microorganism. the sensitivity of the multiplex pcr using purified dna was 25 pg for s. iniae, 12.5 pg for s. difficilis, 50 pg for s. parauberis, and ...200415128589
polymerase chain reaction mediated identification of streptococcus uberis and streptococcus parauberis using species-specific sequences of the genes encoding superoxide dismutase a and chaperonin 60*.streptococcus uberis, a well-known bacterial pathogen associated with bovine mastitis, appears to be biochemically and serologically almost indistinguishable from the closely related species streptococcus parauberis. in the present study, species-specific oligonucleotide primers were designed using internal parts of the genes soda, encoding superoxide dismutase a, and cpn60 encoding chaperonin 60 of s. uberis and s. parauberis, respectively. the two oligonucleotide primer pairs allowed a rapid a ...200415228553
multilocus sequence typing of streptococcus uberis provides sensitive and epidemiologically relevant subtype information and reveals positive selection in the virulence gene paua.control of the bovine mastitis pathogen streptococcus uberis requires sensitive and epidemiologically meaningful subtyping methods that can provide insight into this pathogen's epidemiology and evolution. development of a multilocus sequence typing (mlst) scheme based on six housekeeping and virulence genes allowed differentiation of 40 sequence types among 50 s. uberis isolates from the united states (n = 30) and the netherlands (n = 20). mlst was more discriminatory than ecori or pvuii ribotyp ...200515872274
isolation and characterization of streptococcus sp. from diseased flounder (paralichthys olivaceus) in jeju island.streptococcus sp. is gram-positive coccus that causes streptococcal infections in fish due to intensification of aquaculture and caused significant economic losses in fish farm industry. a streptococcal infection occurred from cultured diseased olive flounder (paralichthys olivaceus) in may, 2005 at a fish farm in jeju island, korea. the diseased flounder exhibited bilateral exophthalmic eyes and rotten gills; water temperature was 16-18 degrees c when samples were collected. of the 22 fish samp ...200616434850
streptococcus ictaluri sp. nov., isolated from channel catfish ictalurus punctatus broodstock.a streptococcal-like organism was associated with diseased channel catfish ictalurus punctatus broodstock on four commercial aquaculture operations in the mississippi delta. conventional biochemical testing, 16s rrna gene sequence analysis and dna-dna hybridization distinguished the isolates from these fish from previously published streptococcus species. comparative 16s rrna gene sequencing studies revealed that the isolates were phylogenetically most similar to streptococcus iniae, streptococc ...200717625202
method for reliable isolation of lactobacillus sakei strains originating from tunisian seafood and meat products.in tunisia, several food products derived from meat or seafood are naturally processed, without any addition of bacterial starters. such fermented, dried-cured, salted, or marinated products, as well as the raw meat or fish may thus provide a source to isolate the natural microflora colonizing such environments. we isolated lactic acid bacteria from a representative range of flesh-foods sold or manufactured in different parts of tunisia, and selectively searched for lactobacillus sakei, a lactic ...200818155310
streptococcus parauberis associated with modified atmosphere packaged broiler meat products and air samples from a poultry meat processing plant.lactic acid bacteria (lab) isolated from marinated or non-marinated, modified atmosphere packaged (map) broiler leg products and air samples of a large-scale broiler meat processing plant were identified and analyzed for their phenotypic properties. previously, these strains had been found to be coccal lab. however, the use of a 16 and 23s rrna gene rflp database had not resulted in species identification because none of the typically meat-associated lab type strains had clustered together with ...200616271788
antibiotic susceptibility and resistance of streptococcus iniae and streptococcus parauberis isolated from olive flounder (paralichthys olivaceus).the rates of antibiotic susceptibility and resistance were investigated in streptococcus iniae and streptococcus parauberis isolates obtained from diseased olive flounders (paralichthys olivaceus) collected from fish farms in jeju island, korea. isolates of s. iniae (n=65) were susceptible to cefotaxime, erythromycin, ofloxacin, penicillin, tetracycline and vancomycin, as demonstrated by the minimum inhibitory concentration (mic) test. isolates of s. parauberis (n=86) were highly resistant to er ...200919019569
genetic analysis of turbot pathogenic streptococcus parauberis strains by ribotyping and random amplified polymorphic dna.ribotyping and rapd profiling of a collection of 18 streptococcus parauberis strains isolated from diseased turbot in galicia (nw spain) was performed in order to analyze the possible genetic variability within this bacterial fish pathogen. in addition, the value of this technique for intraspecific classification and epidemiological studies was evaluated. ribopatterns of dna digested with three endonucleases and hybridized with a cdna probe complementary to highly conserved sequences in the 16s ...199910518729
structural characterization of tn916-like element in streptococcus parauberis serotype ii strains isolated from diseased japanese flounder.to screen for the existence and determine the structure of tn916-like element in streptococcus parauberis serotype ii strains isolated from cultured japanese flounder in western japan.200919344360
pathogen detection in milk samples by ligation detection reaction-mediated universal array method.this paper describes a new dna chip, based on the use of a ligation detection reaction coupled to a universal array, developed to detect and analyze, directly from milk samples, microbial pathogens known to cause bovine, ovine, and caprine mastitis or to be responsible for foodborne intoxication or infection, or both. probes were designed for the identification of 15 different bacterial groups: staphylococcus aureus, streptococcus agalactiae, nonaureus staphylococci, streptococcus bovis, strepto ...200919528580
technology-induced selection towards the spoilage microbiota of artisan-type cooked ham packed under modified atmosphere.the microbiota associated with a highly-perishable belgian artisan-type cooked ham was analyzed through plating and (gtg)(5)-fingerprinting of isolates throughout its processing chain. the raw tumbled meat was characterized by the presence of a versatile microbiota around 4.8 log(cfu g(-1)), consisting of lactic acid bacteria, staphylococci, brochothrix thermosphacta, gram-negative bacteria, and yeasts. pasteurisation of the ham logs reduced bacterial counts below 2 log(cfu g(-1)) and subsequent ...201019913696
lactobacillus sakei bk19 enriched diet enhances the immunity status and disease resistance to streptococcosis infection in kelp grouper, epinephelus bruneus.the effect of lactobacillus sakei bk19 (10(8) cells g(-1)) supplemented diet fed to kelp grouper, epinephelus bruneus against streptococcosis caused by streptococcus iniae and streptococcus parauberis with reference to the innate immune response and disease resistance was evaluated at 1, 2, and 4 weeks. maximum reduction in mortalities was observed in kelper feeding the probiotic diet for two weeks after challenged with the pathogens when compared to the infected group fed with basal diet; simil ...201020816809
development of gene probes for the specific identification of streptococcus uberis and streptococcus parauberis based upon large subunit rrna gene sequences.23s ribosomal rna (rrna) gene sequences of streptococcus uberis and strep. parauberis were determined by direct polymerase chain reaction (pcr) sequencing. oligonucleotide probes specific for strep. uberis and strep. parauberis were designed from variable regions of the 23s rrna gene sequence data. molecular hybridizations with pcr-amplified rrna gene targets provided a precise and reliable means of differentiating strep. uberis and strep. parauberis from each other and from other streptococcal ...19937683638
probiotics and herbal mixtures enhance the growth, blood constituents, and nonspecific immune response in paralichthys olivaceus against streptococcus parauberis.the present study was reported the effect of probiotics and herbals mixture supplementation diet on growth, blood constituents, and nonspecific immune response in olive flounder paralichthys olivaceus against streptococcus parauberis on weeks 1, 2, 4, 6, 8, 10, and 12 after injected intraperitoneally (i.p.) with 50 ╬╝l of pbs (phosphate buffer saline) containing s. parauberis (2.1 ├ù 10ôüà cfu mlôü╗┬╣). the initial weight did not significantly increased in supplementation diet group from 1 to 4 ...201121669289
Prevalence and different characteristics of two serotypes of Streptococcus parauberis isolated from the farmed olive flounder, Paralichthys olivaceus (Temminck and Schlegel), in Korea.The prevalence of two serotypes of Streptococcus parauberis isolated from the olive flounder, Paralichthys olivaceus, was evaluated in a total of 29 isolates between 2003 and 2010 in Korea. Streptococcus parauberis isolates were divided into two serologically distinct types (serotype 1 and serotype 2), except for one strain (S1091), using an agglutination assay with rabbit antiserum, and serotype 1 was identified as the dominant type (24 of 29 isolates) in this study. To identify the characteris ...201121916898
identification and antimicrobial resistance of streptococcus uberis and streptococcus parauberis isolated from bovine milk samples.the conventional identification of streptococcus uberis/parauberis group (n = 137) in clinical and subclinical bovine mastitis samples originating from 111 different farms was compared with identification based on 16 and 23s rrna gene hindiii rflp patterns used as operational taxonomic units in numerical analyses. on the basis of ribopattern analysis only 2 isolates belonged to s. parauberis, which is thus not a frequent cause of bovine intramammary infections in finland. according to in vitro a ...200818832235
streptococcus hongkongensis sp. nov., isolated from a patient with an infected puncture wound and from a marine flatfish.a bacterium, hku30(t), was isolated from the infected tissue of a patient with wound infection after puncture by a fish fin. cells are facultative anaerobic, non-spore-forming, non-motile, gram-positive cocci arranged in chains. colonies were non-haemolytic. the strain was catalase, oxidase, urease and voges-proskauer test negative. it reacted with lancefield's group g antisera and was resistant to optochin. it grew on bile aesculin agar and in 5 % nacl. it was unidentified by three commercial i ...201223264498
microbial diversity and dynamics during the production of may bryndza cheese.diversity and dynamics of microbial cultures were studied during the production of may bryndza cheese, a traditional slovak cheese produced from unpasteurized ewes' milk. quantitative culture-based data were obtained for lactobacilli, lactococci, total mesophilic aerobic counts, coliforms, e. coli, staphylococci, coagulase-positive staphylococci, yeasts, fungi and geotrichum spp. in ewes' milk, curd produced from it and ripened for 0 - 10 days, and in bryndza cheese produced from the curd, in th ...201424291178
characterization of bacterial community of raw milk from dairy cows during subacute ruminal acidosis challenge by high-throughput sequencing.four cannulated primiparous holstein dairy cows (84 ± 25 dim) were used in a 2 × 2 crossover experimental design. the two diets contained 40% (low-concentrate diet, or control diet, lc) and 70% (high-concentrate diet, or sara induction diet, hc) concentrate feeds respectively. milk samples were collected on days 17, 18 and 19 of each experimental period. dna was extracted from each milk sample, and pyrosequencing was applied to analyse the milk microbial community.201524961605
evaluation of marine bacteriocinogenic enterococci strains with inhibitory activity against fish-pathogenic gram-negative bacteria.use of lactic acid bacteria (lab) as probiotics may provide an alternative to the use of antibiotics in aquaculture. lab strains isolated from wild fish viscera and skin were evaluated for bacteriocin production and safety aspects (lack of antibiotic resistance, production of virulence factors). 16s rrna gene sequences revealed the presence of enterococcus faecium (13 isolates) and lactococcus lactis (3 isolates) from fish samples. pulsed-field gel electrophoresis analyses of the 13 enterococci ...201626865233
pharmacokinetics, pharmacokinetic-pharmacodynamic relationship, and withdrawal period of amoxicillin sodium in olive flounder (paralichthys olivaceus).1. the pharmacokinetics (pk) and withdrawal period of amoxicillin sodium in olive flounder and its activity against pathogenic bacteria of olive flounder were investigated. 2. intramuscular administration (12.5 or 125 mg/kg, n = 160) and hplc analysis of sera were used. 3. rapid absorption (tmax 2.6 and 2.2 h), prolonged action (terminal half-life, 15.52 and 10.42 h; mrt, 18.79 and 14.44 h), and dose-proportional exposure (auc0-∞, 273.69 and 2755.37 h. μg/ml) were observed after 12.5 and 125 mg/ ...201626407038
development of a multiplex pcr assay to detect edwardsiella tarda, streptococcus parauberis, and streptococcus iniae in olive flounder (paralichthys olivaceus).a multiplex pcr protocol was established to simultaneously detect major bacterial pathogens in olive flounder (paralichthys olivaceus) including edwardsiella (e.) tarda, streptococcus (s.) parauberis, and s. iniae. the pcr assay was able to detect 0.01 ng of e. tarda, 0.1 ng of s. parauberis, and 1 ng of s. iniae genomic dna. furthermore, this technique was found to have high specificity when tested with related bacterial species. this method represents a cheaper, faster, and reliable alternativ ...201424378589
microbiological characterization using combined culture dependent and independent approaches of casizolu pasta filata cheese.casizolu is a traditional sardinian (italy) pasta filata cheese made with cow raw milk belonging to sardo-modicana and/or bruno-sarda breeds added with natural whey starter. this work aims to describe the traditional technology of this product and to evaluate the microbial groups/species involved in the first month of ripening.201626551888
complete genome sequence and immunoproteomic analyses of the fish bacterial pathogen streptococcus parauberis.although streptococcus parauberis is known as a bacterial pathogen associated with bovine udder mastitis, it has recently become one of the major causative agents of olive flounder (paralichthys olivaceus) streptococcosis in northeast asia, causing massive mortality resulting in severe economic losses. s. parauberis contains two serotypes, and it is likely that capsular polysaccharide antigens serve to differentiate the serotypes. in the present study, the complete genome sequence of s. parauber ...201121531805
isolation and characterization of streptococcus parauberis from vacuum-packaging refrigerated seafood products.streptococcus parauberis is known as an etiological agent of mastitis in cows and for producing streptococcosis in farmed fish, although its presence in foods has seldom been reported. in this work, two bacterial isolates were recovered from a spoiled vacuum-packaged refrigerated seafood product. both isolates were identified by 16s rrna gene sequencing, exhibiting 99% homology with respect to s. parauberis. both isolates were also characterized by matrix-assisted laser desorption/ionization tim ...201122265288
protective efficacy of streptococcus iniae derived enolase against streptococcal infection in a zebrafish model.enolase (eno) is one of the surface-exposed proteins of streptococcus iniae, which previously had been identified as a plasminogen-binding protein. in this study, eno was evaluated to induce cross-protective immunity against s. iniae and streptococcus parauberis which are major pathogens causing streptococcosis in fish. immunoblot analysis shows that s. iniae recombinant eno (reno) produced in escherichia coli was cross-reactive with antisera against s. iniae, and s. parauberis serotype i and ii ...201626872628
lactococcus lactis bfe920 activates the innate immune system of olive flounder (paralichthys olivaceus), resulting in protection against streptococcus iniae infection and enhancing feed efficiency and weight gain in large-scale field studies.the protective effect of a food-grade lactic acid bacterium lactococcus lactis bfe920 against disease of olive flounder (paralichthys olivaceus) cultivated on a large scale was studied. initially, antimicrobial activity of l. lactis against several fish pathogens was evaluated in vitro; the probiotic showed strong antibacterial activity against streptococcus iniae, streptococcus parauberis and enterococcus viikkiensis, and moderate activity against lactococcus garviae. when olive flounders were ...201324041843
chromosomal islands of streptococcus pyogenes and related streptococci: molecular switches for survival and virulence.streptococcus pyogenes is a significant pathogen of humans, annually causing over 700,000,000 infections and 500,000 deaths. virulence in s. pyogenes is closely linked to mobile genetic elements like phages and chromosomal islands (ci). s. pyogenes phage-like chromosomal islands (spyci) confer a complex mutator phenotype on their host. spyci integrate into the 5' end of dna mismatch repair (mmr) gene mutl, which also disrupts downstream operon genes lmrp, ruva, and tag. during early logarithmic ...201425161960
microbial diversity of different modified atmosphere packed pot-stewed duck wings products during 8°c storage.the objective of this study was to investigate the effect of spices and packaging films on the bacterial dynamics of pot-stewed duck wings (psdw) during storage at 8°c. the results showed that spices added at a 1 : 1 ratio (weight ratio of pungent spices and fragrant spices) could delay the lag period of bacterial growth and extend the shelf-life of psdw. denaturing gel gradient electrophoresis (dgge) analysis indicated that the predominant bacteria in psdw at the end of storage were weisella he ...201728191705
draft genome sequences for seven streptococcus parauberis isolates from wild fish in the chesapeake bay.streptococcus parauberis is a pathogen of cattle and fish, closely related streptococcus uberis and streptococcus iniae we report the genomes of seven s. parauberis strains recovered from striped bass (morone saxatilis) in the chesapeake bay. the availability of these genomes will allow comparative genomic analysis of chesapeake bay s. parauberis strains versus s. parauberis cultured from other animal hosts and geographic regions.201627540054
effects of phosphoglucomutase gene (pgm) in streptococcus parauberis on innate immune response and pathogenicity of olive flounder (paralichthys olivaceus).in recent years, streptococcus parauberis infection has been an emerging problem in aquaculture in south korea because of its more frequent isolation than other streptococcal bacteria including streptococcus iniae. to develop effective treatment and prophylaxis methods against this emerging disease by s. parauberis, it is necessary to understand the underlying pathogenic mechanisms. to uncover the pathogenicity, the mutant strain of s. parauberis with a deleted phosphoglucomutase (pgm) gene whic ...201425240979
phenotypic characteristics of streptococcus iniae and streptococcus parauberis isolated from olive flounder (paralichthys olivaceus).the etiological agents of streptococcosis were isolated from diseased olive flounder collected on the jeju island of korea. a total of 151 bacterial isolates were collected between 2003 and 2006. the isolates were examined using various phenotypic and proteomic analyses, including sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page), immunoblotting, and glycoprotein assays. in addition, isolates were grown on blood agar to assess hemolytic activity, and biochemical assays were pe ...200919278524
diversity of lactic acid bacteria during fermentation of a traditional chinese fish product, chouguiyu (stinky mandarinfish).chouguiyu, or stinky mandarinfish, is a traditional chinese fermented fish product made of mandarinfish by spontaneous fermentation at the anaerobic condition with low-salt concentration. in order to get a primary understanding of the microbial community presenting in the chouguiyu fermentation, 61 cultures of lactic acid bacteria (lab) from various fermentation period were isolated using mrs agar plates and characterized based on a combination of phenotypic and genotypic approaches including am ...201324245896
development of real-time pcr for detection and quantitation of streptococcus parauberis.streptococcus parauberis is an increasing threat to aquaculture of olive flounder, paralichthys olivaceus temminck & schlegel, in south korea. we developed a real-time polymerase chain reaction (pcr) method using the taqman probe assay to detect and quantify s. parauberis by targeting the gyrb gene sequences, which are effective for molecular analysis of the genus streptococcus. our real-time pcr assay is capable of detecting 10 fg of genomic dna per reaction. the intra- and interassay coefficie ...201625345976
whole-genome sequence of streptococcus parauberis strain sp-llh, isolated from cows with mastitis in western china.streptococcus parauberis strain sp-llh was isolated from cows with mastitis in western china in 2015. the 2,522,235-bp genome sequence consists of 46 large contigs in 14 scaffolds and contains 2,620 predicted protein-coding genes, with a g+c content of 35.3%.201728082485
comparative genomic characterization of three streptococcus parauberis strains in fish pathogen, as assessed by wide-genome analyses.streptococcus parauberis, which is the main causative agent of streptococcosis among olive flounder (paralichthys olivaceus) in northeast asia, can be distinctly divided into two groups (type i and type ii) by an agglutination test. here, the whole genome sequences of two japanese strains (krs-02083 and krs-02109) were determined and compared with the previously determined genome of a korean strain (kctc 11537). the genomes of s. parauberis are intermediate in size and have lower gc contents tha ...201324260382
genome sequence of streptococcus parauberis strain kctc11980, isolated from diseased paralichthys olivaceus.streptococcus parauberis is a coccoid, nonmotile, alpha-hemolytic, gram-positive bacterium of the streptococcaceae family. streptococcus parauberis strain kctc11980 was isolated from the kidney of a diseased olive flounder collected from an aquaculture farm on jeju island in 2010. the 2.12-mb genome sequence consists of 44 large contigs in 16 scaffolds and contains 2,214 predicted protein-coding genes, with a g+c content of 35.4%.201324092782
first report of streptococcus parauberis in wild finfish from north america.streptococcosis is a common cause of pathology and mortality in fishes resulting in significant economic losses for the aquaculture industry. one etiologic agent of the disease, streptococcus parauberis, has been associated with fish mortalities in spain and korea. here we report the first identification of s. parauberis in wild finfish in chesapeake bay, usa. gram-positive cocci were isolated from the spleens of striped bass, morone saxatilis, and identified via species-specific primers and 16s ...201323769635
streptococcous parauberis infection in starry flounder, platichthys stellatus: characterization of innate immune responses following experimental infection.streptococcus parauberis causing systemic infections has been recognized as a major bacterial disease in olive flounder paralichthys olivaceus in south korea. although an emerging outbreak of s. parauberis has affected heavily farmed fish species starry flounder platichthys stellatus, no study of the innate immune responses and pathogenic mechanisms in starry flounder is available. in the present study, starry flounder were intraperitoneally challenged with four s. parauberis strains to investig ...201323707742
heat shock protein profiles on the protein and gene expression levels in olive flounder kidney infected with streptococcus parauberis.heat shock proteins (hsps) have been observed in cells exposed to a variety of stresses, including infectious pathogens. this study used a label-free, quantitative proteomic approach and transcriptional gene expression analysis to investigate infection-related hsp proteins and their encoding genes in whole kidneys from olive flounder (paralichthys olivaceus). during streptococcus parauberis infection in the flounder, the genes encoding hsp10, hsp40a4, hsp40b6, hsp40b11, hsp60, hsp70, glucose reg ...201323542604
cathepsins in the kidney of olive flounder, paralichthys olivaceus, and their responses to bacterial infection.cathepsin activities are responsible for mediating various pathways involved in immune response, including the apoptosis pathway, toll-like receptor (tlr) signaling, cytokine induction and activation of granule serine proteases. in the present study, we investigated cathepsin responses in the kidneys of olive flounder infected with streptococcus parauberis, analyzing cathepsin expression using a label-free, quantitative proteomic approach in conjunction with quantitative real-time polymerase cha ...201223000266
kidney proteome responses in the teleost fish paralichthys olivaceus indicate a putative immune response against streptococcus parauberis.the proteomic response to bacterial infection in a teleost fish (paralichthys olivaceus) infected with streptococcus parauberis was analyzed using label-free protein quantitation coupled with lc-ms(e) tandem mass spectrometry. a total of 82 proteins from whole kidney, a major lymphoid organ in this fish, were found to be differentially expressed between healthy and diseased fish analyzed 6, 24, 72 and 120 h post-infection. among the differentially expressed proteins, those involved in mediating ...201222687251
post-traumatic endophthalmitis caused by streptococcus parauberis: first human case report.a 12-year-old boy presented with trauma to left eye with a wooden stick. on examination, there was full thickness corneal laceration with cataractous lens behind the laceration. the laceration was sutured, and intravitreal injections of vancomycin, ceftazidime and clindamycin were administered. vitreous tap grew streptococcus parauberis. the isolate was sensitive to amoxicillin, erythromycin and vancomycin, and topical vancomycin was used to treat the infection. we present the first case of huma ...201727514967
comparison of proteome typing and serotyping of streptococcus parauberis isolates from olive flounder (paralichthys olivaceus).the olive flounder (paralichthys olivaceus) is a cultivated marine species that is economically important in korea and japan. several bacterial pathogens have caused severe mortalities in farmed olive flounder, especially streptococcus parauberis. we collected 145 s. parauberis isolates from diseased olive flounders from 2003 to 2008 in jeju island, south korea and characterized them by matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi tof ms) and by serology. t ...201526423006
draft genome sequence of streptococcus parauberis strain sk-417, isolated from diseased sebastes ventricosus in kagoshima, japan.streptococcus parauberis strain sk-417 was isolated from the brain of a diseased sebastes ventricosus, collected from an aquaculture farm in april 2013 in kagoshima prefecture, japan. the draft genome sequence, obtained with a 454 gs junior sequencing system, consists of 33 large contigs of >500 bp, totaling 1,958,836 bp, and has a g+c content of 35.4%.201424855302
first report of streptococcus parauberis in a cultured freshwater ornamental fish, the ram cichlid mikrogeophagus ramirezi (myers & harry, 1948). 201728699689
interferon-induced protein 56 (ifi56) is induced by vhsv infection but not by bacterial infection in olive flounder (paralichthys olivaceus).interferon-inducible protein 56 (ifi56, also known as isg56/ifit1, interferon-induced protein with tetratricopeptide repeats 1) is strongly induced in response to interferon and a potent inhibitor of viral replication and translational initiation. here, we describe the identification of ifi56 (ofifi56) in olive flounder, its characteristic features, and expression levels in various tissues before and after viral hemorrhagic septicemia virus (vhsv) infection. the full-length ofifi56 sequence was ...201728499966
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