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urea concentration in minor mucous gland secretions and the effect of salivary film velocity on urea metabolism by streptococcus vestibularis in an artificial plaque.our purpose was to determine the urea concentration in minor mucous gland (mmg) secretions and the ph at proximal and distal aspects of the lower surface of artificial plaque in vitro during infusion of urea solutions over the surface, at different film velocities. saliva is present in the mouth as a slowly moving film (ca. 0.1 mm thick) with an estimated velocity in the range of 0.8-8.0 mm/min. at low velocities, due to the accumulation of bacterial products, a progressive increase in their con ...19911832451
ability to bind salivary alpha-amylase discriminates certain viridans group streptococcal species.a collection of 144 viridans group streptococcal strains recently characterized as part of a taxonomic study was examined for the ability to bind salivary alpha-amylase. this property was found in most strains of streptococcus gordonii and streptococcus mitis and in occasional strains of streptococcus anginosus and streptococcus salivarius. in contrast, all strains of streptococcus sanguis, streptococcus oralis, streptococcus vestibularis, and streptococcus mutans lacked alpha-amylase-binding ca ...19902254435
distribution of proteins similar to iiimanh and iiimanl of the streptococcus salivarius phosphoenolpyruvate:mannose-glucose phosphotransferase system among oral and nonoral bacteria.in streptococcus salivarius, the phosphoenolpyruvate (pep):mannose-glucose phosphotransferase system, which concomitantly transports and phosphorylates mannose, glucose, fructose, and 2-deoxyglucose, is composed of the general energy-coupling proteins ei and hpr, the specific membrane-bound iiiman, and two forms of a protein called iiiman, with molecular weights of 38,900 (iiimanh) and 35,200 (iiimanl), that are found in the cytoplasm as well as associated with the membrane. several lines of evi ...19957730253
streptococcus vestibularis bacteraemia. 19979120333
effect of low-molecular-weight chitosans on the adhesive properties of oral streptococci.it was previously shown that a low-molecular-weight chitosan and its derivatives n-carboxymethyl chitosan and imidazolyl chitosan inhibit streptococcus mutans adsorption to hydroxyapatite. the ability of the same molecules to interfere with adhesive properties of other oral streptococci (streptococcus sanguis, streptococcus gordonii, streptococcus constellatus, streptococcus anginosus, streptococcus intermedius, streptococcus oralis, streptococcus salivarius, streptococcus vestibularis) was test ...199810093536
identification of clinically relevant viridans streptococci by analysis of transfer dna intergenic spacer length polymorphism.the utility of pcr analysis of transfer dna intergenic spacer length polymorphism (tdna-ilp) for the identification to the species level of clinically relevant viridans streptococci was evaluated with a collection of reference strains of 15 species of the salivarius, anginosus, mitis and mutans rrna homology groups. pcr products generated by using fluorescent, outwardly directed, consensus tdna primers were analysed by electrophoresis on denaturating polyacrylamide gels and by laser fluorescence ...199910555340
prosthetic valve endocarditis due to streptococcus vestibularis. 200011120623
salivary concentrations of urea released from a chewing gum containing urea and how these affect the urea content of gel-stabilized plaques and their ph after exposure to sucrose.the objectives were to: (1) determine the salivary concentrations of urea during 20 min chewing of a sugar-free gum containing 30 mg of urea; (2) measure the degree to which this urea would diffuse into a gel-stabilized plaque; (3) study the effect of the urea on the fall and subsequent rise in ph (stephan curve) on exposure to 10% sucrose for 1 min; (4) model the measurements 2 and 3 mathematically. in point 1, the salivary urea concentration of the 12 subjects peaked at 47 mmol/l in the first ...200111641570
native valve endocarditis due to streptococcus vestibularis and streptococcus oralis.viridans streptococci are the commonest cause of native valve infective endocarditis (ie). the taxonomy of this group is evolving allowing new disease associations to be made. streptococcus vestibularis is a recently described member of the viridans group, first isolated from the vestibular mucosa of the human oral cavity. it has rarely been associated with human disease. streptococcus oralis, another member of the viridans group resident in the human oral cavity is a well known cause of ie and ...200212217730
evolutionary relationships among salivarius streptococci as inferred from multilocus phylogenies based on 16s rrna-encoding, reca, seca, and secy gene sequences.streptococci are divided into six phylogenetic groups, i.e, anginosus, bovis, mitis, mutans, pyogenic, and salivarius, with the salivarius group consisting of only three distinct species. two of these species, streptococcus salivarius and streptococcus vestibularis, are members of the normal human oral microflora whereas the third, streptococcus thermophilus, is found in bovine milk. given that s. salivarius and s. vestibularis share several physiological characteristics, in addition to inhabiti ...200919878555
spondylodiscitis and endocarditis caused by s. vestibularis.streptococcus vestibularis is a recently described member of the viridans group that was first isolated from the vestibular mucosa of the human oral cavity and described as a new species in 1988. it has been rarely associated with human infections. in few papers, it has been reported as a causal agent of systemic infection in immunosupressed adults and in those with other severe underlying diseases, like coronary valve diseases. a 65-year-old woman was admitted to the hospital with complaints of ...201020963324
phylogenetic analyses and detection of viridans streptococci based on sequences and denaturing gradient gel electrophoresis of the rod shape-determining protein gene.population analysis of viridans streptococci is important because these species are associated with dental caries, bacteremia, and subacute endocarditis, in addition to being important members of the human oral commensal microbiota.200921523207
differential utilization of basic proline-rich glycoproteins during growth of oral bacteria in saliva.although saliva is widely recognized as a primary source of carbon and nitrogen for growth of the dental plaque biofilm community, little is known about how different oral bacteria utilize specific salivary components. to address this question, 32 strains representing 16 genera commonly isolated from early plaque biofilms were compared for growth over two transfers in stimulated (by chewing parafilm) whole saliva that was stabilized by heat treatment and dialysis. the cell densities, measured by ...201627316966
evaluation of an ethidium monoazide-enhanced 16s rdna real-time polymerase chain reaction assay for bacterial screening of platelet concentrates and comparison with automated culture.culture-based systems are currently the preferred means for bacterial screening of platelet (plt) concentrates. alternative bacterial detection techniques based on nucleic acid amplification have also been developed but these have yet to be fully evaluated. in this study we evaluate a novel 16s rdna polymerase chain reaction (pcr) assay and compare its performance with automated culture.201423701338
an examination of strains of the bacterium streptococcus vestibularis for relative cariogenicity in gnotobiotic rats and adhesion in vitro.the cariogenicity and adhesion of six strains of streptococcus vestibularis were compared with those of strains of strep. salivarius. all strains of strep. vestibularis produced low levels of caries, confined to the fissures, whereas the two strains of strep. salivarius produced high levels of caries, with one strain producing approximal as well as fissure caries. the values for adhesion to saliva-coated hydroxyapatite of strep. vestibularis in the absence (median 4.74%) and the presence (median ...19911651693
identification of clinically relevant nonhemolytic streptococci on the basis of sequence analysis of 16s-23s intergenic spacer region and partial gdh gene.nonhemolytic streptococci (nhs) cause serious infections, such as endocarditis and septicemia. many conventional phenotypic methods are insufficient for the identification of bacteria in this group to the species level. genetic analysis has revealed that single-gene analysis is insufficient for the identification of all species in this group of bacteria. the aim of the present study was to establish a method based on sequence analysis of the 16s-23s intergenic spacer (its) region and the partial ...200919193846
assessment of inhibitory potency of antibiotics by mri: apparent t2 as a marker of cell growth.a new method to assess the antibiotic potency by mri has been developed. correlating 1h nmr spectra of bacterial cultures with the extracellular parameters t2, od600, and ph, a relationship between cell growth and t2 variations was established. t2 is influenced by chemical exchange that depends on ph, composition, and concentration of the medium. changes in the medium from bacterial metabolism are reflected in alternating t2 values. at 17.6 t, growth curves based on t2 values were measured simul ...200617096123
extent of horizontal gene transfer in evolution of streptococci of the salivarius group.the phylogenetically closely related species streptococcus salivarius and streptococcus vestibularis are oral bacteria that are considered commensals, although they can also be found in human infections. the relationship between these two species and the relationship between strains isolated from carriers and strains responsible for invasive infections were investigated by multilocus sequence typing and additional sequence analysis. the clustering of several s. vestibularis alleles and the exten ...200717085557
identification of emerging human mastitis pathogens by maldi-tof and assessment of their antibiotic resistance patterns.lactational mastitis constitutes one of the main causes of undesired weaning, depriving the mother-infant pair from the benefits of breastfeeding; therefore, this condition should be considered a relevant public health issue. the role of specific microorganisms remains unclear since human milk cultures and antibiotic susceptibility testing (ast) are not routinely performed, despite the fact that this would be key to ensure an early and effective diagnosis and treatment. the objective of this stu ...201728747897
clustered regularly interspaced short palindrome repeats (crisprs) have spacers of extrachromosomal origin.numerous prokaryote genomes contain structures known as clustered regularly interspaced short palindromic repeats (crisprs), composed of 25-50 bp repeats separated by unique sequence spacers of similar length. crispr structures are found in the vicinity of four genes named cas1 to cas4. in silico analysis revealed another cluster of three genes associated with crispr structures in many bacterial species, named here as cas1b, cas5 and cas6, and also revealed a certain number of spacers that have ...200528202105
streptococcus loxodontisalivarius sp. nov. and streptococcus saliviloxodontae sp. nov., isolated from oral cavities of elephants.four gram-stain-positive, catalase-negative, coccoid-shaped organisms were isolated from elephant oral cavities. the isolates were tentatively identified as streptococcal species based on the results of biochemical tests. comparative 16s rrna gene sequencing studies confirmed the organisms to be members of the genus streptococcus. two isolates (num 6304(t) and num 6312) were related most closely to streptococcus salivarius with 96.8 % and 93.1 % similarity based on the 16s rrna gene and the rna ...201424994777
genomics of streptococcus salivarius, a major human commensal.the salivarius group of streptococci is of particular importance for humans. this group consists of three genetically similar species, streptococcus salivarius, streptococcus vestibularis and streptococcus thermophilus. s. salivarius and s. vestibularis are commensal organisms that may occasionally cause opportunistic infections in humans, whereas s. thermophilus is a food bacterium widely used in dairy production. we developed multilocus sequence typing (mlst) and comparative genomic analysis t ...201525311532
using pcr-based detection and genotyping to trace streptococcus salivarius meningitis outbreak strain to oral flora of radiology physician assistant.we recently investigated three cases of bacterial meningitis that were reported from a midwestern radiology clinic where facemasks were not worn during spinal injection of contrast agent during myelography procedures. using pulsed field gel electrophoresis we linked a case strain of s. salivarius to an oral specimen of a radiology physician assistant (rpa). we also used a real-time pcr assay to detect s. salivarius dna within a culture-negative cerebrospinal fluid (csf) specimen. here we extend ...201222384169
streptococcus vestibularis bacteremia following dental extraction in a patient on long-term hemodialysis: a case report. 200825983909
"streptococcus vestibularis": a rare cause of peritoneal dialysis-related peritonitis. 201728321985
clustered regularly interspaced short palindrome repeats (crisprs) have spacers of extrachromosomal origin.numerous prokaryote genomes contain structures known as clustered regularly interspaced short palindromic repeats (crisprs), composed of 25-50 bp repeats separated by unique sequence spacers of similar length. crispr structures are found in the vicinity of four genes named cas1 to cas4. in silico analysis revealed another cluster of three genes associated with crispr structures in many bacterial species, named here as cas1b, cas5 and cas6, and also revealed a certain number of spacers that have ...200516079334
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