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adherence of glucan-positive and glucan-negative strains of streptococcus bovis to human epithelial cells.adherence to buccal epithelial cells (bec) and the role played in the binding by lipoteichoic acid (lta) and other superficial components have been studied in reference and clinical strains of streptococcus bovis either glucan-positive biotype i or glucan-negative biotype ii. to avoid the synthesis of glucan by biotype i strains, adherence was studied in bacteria grown in todd-hewitt broth, a sucrose deficient medium. both biotypes were shown to bind to bec and clinical isolates, irrespective of ...19938392108
scleral flap necrosis and infectious endophthalmitis after cataract surgery with a scleral tunnel incision.long scleral tunnel dissection techniques have been developed for cataract surgery incisions. these incisions reduce postoperative astigmatism and keratorefractive instability. if fashioned correctly, the internal lip of the incision produces a tight seal to the anterior chamber, permissible of sutureless surgery. the behavior of such a wound during intraocular infection is unknown.19938437821
significance of streptococcus bovis endocarditis. 19938442633
[unusual presentation of streptococcus bovis endocarditis]. 19938474289
[the characteristics of the regulation of lysine biosynthesis in streptococcus bovis].the effect of threonine and methionine on the growth of and lysine synthesis in streptococcus bovis st. a024/85 was studied. the character and the degree of manifestation of growth and regulation effects of these amino acids depend on their contents in the fermentation medium. the target in the regulation of lysine biosynthesis is aspartate kinase, whose synthesis is controlled by threonine and methionine. lysine excretion was stimulated by the addition of a low concentration of dimethylsulfoxid ...19938488160
[occurrence of streptococci resistant to antibiotics in the rumen of calves].the occurrence and antibiotic-resistance spectrum of some strains of rumen streptococci were investigated in calves of four age categories in the milk diet period at 1-3, 4-7, 8-14 days of age and 6-8 weeks of age. more than 75% of the strains isolated from the calves of all age categories were resistant to more than one tested antibiotic (tetracycline, neomycin, erythromycin, streptomycin, chloramphenicol). the average number of streptococci isolated from the ingesta of calves during the period ...19938488631
the antimicrobial activity and beta-lactamase stability of cefpirome, a new fourth-generation cephalosporin in comparison with other agents.the antimicrobial activity of cefpirome was compared with amoxycillin/clavulanic acid, ampicillin/sulbactam, cefuroxime, ceftazidime, gentamicin and amikacin against 743 non-duplicate clinical isolates. mic50 and mic90 showed that the antibiotic was active against both gram-negative and gram-positive organisms. cefpirome was highly active against most of the enterobacteriaceae, including indole-positive proteus spp., aeromonas spp. (mic < or = 1 mg/l) and salmonella spp. (mic < or = 0.5 mg/l). n ...19938335498
in vitro effects of a thiopeptide and monensin on ruminal fermentation of soluble carbohydrates.using a purified diet and a mixed culture of ruminal microorganisms, we studied the in vitro effects of a sulfur-containing peptide (a10255) and monensin on ruminal vfa and lactic acid concentrations. after 22 h of fermentation, total vfa concentration was greater in cultures with a10255 and monensin (2.5 ppm each) than in untreated controls (84.1, 91.9, and 65.2 mm for cultures with a10255, monensin, and control, respectively). the molar proportions of propionate were more than 40% greater, and ...19938486839
probiotics and manipulation of rumen development and function.there is good evidence that the bacterial and fungal probiotics are effective in the manipulation of rumen development and function. the effects of adherent streptococcus bovis preparations were manifested by significant increase of adherent s. bovis bacteria and alpha amylase activity in the rumen wall of young ruminants. fungal probiotics based on saccharomyces cerevisiae and aspergillus oryzae are rapidly gaining acceptance as a means of improving productivity in adult ruminants. however, fun ...19938215880
streptococcus bovis meningitis: no association with colonic malignancy. 19938218715
a case of infective endocarditis in a farmer caused by streptococcus equinus.the following is a case report of a farmer with documented aortic valve disease who developed bacterial endocarditis secondary to streptococcus equinus. this is a rare pathogen in man and its acquisition in this case may be related to the subject's occupation.19938223744
[pulmonary valve endocarditis, bilateral deep vein thrombosis amd recurrent pulmonary emboli].a 57-year-old obese man with hypertensive heart disease and long-standing varicose veins developed recurrent bouts of fever, lung infiltrations, cardiac arrhythmias and increasing dyspnoea. on admission multiple lung emboli and bilateral deep vein thromboses were confirmed. the echocardiogram demonstrated floating vegetations on all three pulmonary valve cusps. biochemical tests indicated an inflammatory constellation (esr 62/105 mm, c-reactive protein 13.3 mg/dl), partial respiratory insufficie ...19938243249
comparative effect of salinomycin and monensin on streptococcus bovis strain atcc 9809.the effect of salinomycin (2.5 mg/l) on the in vitro growth of streptococcus bovis strain atcc 9809, on its catabolism of glucose and on its anabolism of lactic acid was compared with that of monensin (5.0 mg/l). in control cultures containing no ionophore, glucose consumption and lactic acid production were completed concomitantly with maximal growth which was reached after 2 h of incubation at 37 degrees c. no further growth of s. bovis was observed within 2 h after the addition of one or the ...19938264432
the inhibition by ionophores in vitro of an enterococcus-like pathogen of rainbow trout, oncorhynchus mykiss.streptococcosis is a major disease of several fish species in australia, japan and south africa. the minimum inhibitory concentration of some ionophores (lasalocid, monensin, narasin and salinomycin) was determined in vitro for an enterococcus-like species pathogenic for rainbow trout (oncorhynchus mykiss) in australia. forty isolates of the fish pathogen were tested, together with control strains of staphylococcus aureus atcc 29213, enterococcus faecalis atcc 29212 and streptococcus bovis atcc ...19938273272
serological investigation into the association between streptococcus bovis and colonic cancer.to determine if there was an increase in antibody titre to streptococcus bovis in patients with colonic cancer, and if this might be a useful marker of the presence for colonic cancer.19938282836
prevalence of antibodies to streptococcus bovis serotype 1 in racing pigeons.indirect elisa techniques for the detection of antibodies to s. bovis serotypes 1, 2 and 3 in pigeon plasma were developed. whole formaldehyde-inactivated bacteria were used as coating antigens. bound antibodies were detected with peroxidase-conjugated rabbit anti-pigeon serum and opd and h2o2 as chromogen and substrate, respectively. elisa was used to determine the prevalence of antibodies to s. bovis serotype 1 in healthy belgian racing pigeons. antibodies were demonstrated in 83 (= 37%) of 22 ...19938284963
[association of streptococcus bovis endocarditis and liver cirrhosis]. 19938511149
purification and characterization of the extracellular alpha-amylase from streptococcus bovis jb1.the extracellular alpha-amylase (1,4-alpha-d-glucanglucanohydrolase; ec 3.2.1.1) from maltose-grown streptococcus bovis jb1 was purified to apparent homogeneity by ion-exchange chromatography (mono q). the enzyme had an isoelectric point of 4.50 and an apparent molecular mass of 77,000 da, as estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. the enzyme was rich in acidic and hydrophobic amino acids. the 15-amino-acid nh2-terminal sequence was 40% homologous with the bacillu ...19938517735
comparison of fluorescent gentamicin-thallous-carbonate and kf streptococcal agars to enumerate enterococci and fecal streptococci in meats.two selective and differential media were compared for their abilities to enumerate enterococci and fecal streptococci in pork, beef, and poultry products. counts obtained on kf streptococcal (kf) agar were compared with counts obtained on fluorescent gentamicin-thallous-carbonate (fgtc) agar. reactions of 13 known enterococcal species were also observed. all 13 species of enterococci as well as streptococcus bovis and streptococcus equinus grew equally well on fgtc agar. kf streptococcal medium ...19938481014
suspicious mind: the association between streptococcus bovis endocarditis and carcinoma of the colon. 19938497446
antibiotic treatment of streptococcus bovis infections in pigeons.the antibiotic susceptibility pattern of 5. bovis strains isolated from pigeons was studied. in vitro, s. bovis strains were sensitive to penicillins, macrolides, lincomycin, tetracyclines, chloramphenicol and nitrofurans. however, the prevalence of acquired resistance against tetracyclines was approximately 40%. sulphonamides and trimethoprim had little in vitro activity against 5. bovis while activity of the quinolone enrofloxacin and the aminoglycoside antibiotics, neomycin and gentamicin, we ...199318671044
effects of sainfoin (onobrychis viciifolia scop.) condensed tannins on growth and proteolysis by four strains of ruminal bacteria.sainfoin leaf condensed tannins inhibited growth and protease activity in butyrivibrio fibrisolvens a38 and streptococcus bovis 45s1 but had little effect on prevotella ruminicola b(1)4 or ruminobacter amylophilus wp225. tannins bound to cell coat polymers in all strains. morphological changes in b. fibrisolvens and s. bovis implicated the cell wall as a target of tannin toxicity.199416349244
substrate preference in a strain of megasphaera elsdenii, a ruminal bacterium, and its implications in propionate production and growth competition.the niah 1102 strain of megasphaera elsdenii utilized lactate in preference to glucose when the two substrates were present. even when lactate was supplied to cells fermenting glucose, the cells switched substrate utilization from glucose to lactate and did not utilize glucose until lactate decreased to a low concentration (1 to 2 mm). since substrate utilization was shifted gradually without intermittence, typical diauxic growth was not seen. the cyclic amp content did not rise markedly with th ...199416349276
nutrient transport by ruminal bacteria: a review.fermentation pathways have been elucidated for predominant ruminal bacteria, but information is limited concerning the specific transport mechanisms used by these microorganisms for c, energy, and n sources. in addition, it is possible that changes in ruminal environmental conditions could affect transport activity. five carrier-mediated soluble nutrient transport mechanisms have been identified in bacteria: 1) facilitated diffusion, 2) shock sensitive systems, 3) proton symport, 4) na+ symport, ...19947730197
conjugative transfer of tetracycline resistance in rumen streptococcal strains.in 11% of tested streptococcus bovis strains a conjugative transfer of tetracycline resistance was observed when mating experiments were carried out on membrane filters. the recipient strain used was s. bovis bm114 with chromosomal resistance to rifampicin. in addition, in two strains tetracycline resistance was transferred also to recipient strain enterococcus faecium al6. the transfer frequencies were in the range of 10(-6) to 10(-3). the donor strains were screened for the presence of plasmid ...19948181787
[lumbago and constitutional symptoms in a 79-year-old man]. 19948183001
streptococcus bovis endocarditis presenting as acute spondylodiscitis. 19948156317
streptococcus bovis bacteremia and meningitis associated with strongyloides stercoralis colitis in a patient infected with human immunodeficiency virus.we present a case of human immunodeficiency virus (hiv) infection complicated by streptococcus bovis meningitis and bacteremia and severe strongyloides stercoralis colitis. the association between s. bovis infection and strongyloidiasis has not been described previously. this case highlights the importance of searching for larvae of s. stercoralis as part of the evaluation of the gastrointestinal tract of patients with bacteremia or meningitis due to certain enteric organisms. the role of hiv in ...19948161638
[pulmonary valve endocarditis caused by streptococcus bovis]. 19948162846
energy-spilling reactions of streptococcus bovis and resistance of its membrane to proton conductance.glucose-excess cultures of streptococcus bovis consumed glucose faster than the amount that could be explained by growth or maintenance, and nongrowing chloramphenicol-treated cells had a rate of glucose consumption that was 10-fold greater than the maintenance rate. because n,n-dicyclohexylcarbodiimide, an inhibitor of the membrane-bound f1f0 atpase, eliminated the nongrowth energy dissipation (energy spilling) without a decrease in atp and the rate of energy spilling could be increased by the ...19948031089
endocarditis associated with anca.we report the case of a patient exhibiting subacute bacterial endocarditis (seb) associated with vasculitis and signs of nephritis. c-anca were present at high titer. this observation suggests that anca may be associated with vascular injury in seb.19948039291
[bacteremia caused by streptococcus salivarius and s. milleri, and colonic carcinoma].there is a well-known association between colon carcinoma and bacteraemia with streptococcus bovis biotype i. there are also associations, less well known, with other streptococci of the viridans group. in three patients, a man of 44, a woman of 53 and one of 52 years old, colorectal carcinomas were diagnosed in association with bacteraemia with s. salivarius, s. milleri, and s. salivarius respectively.19948065473
binding of radiolabeled monensin and lasalocid to ruminal microorganisms and feed.gram-negative, ionophore-resistant ruminal bacteria and gram-positive, ionophore-sensitive species bound similar amounts of [14c]lasalocid, but neither group bound large amounts of [14c]monensin. membrane vesicles also bound more lasalocid than monensin (p < .05). the binding was first-order at low cell or vesicle concentrations and saturable at high cell or vesicle densities. streptococcus bovis was inhibited by both monensin and lasalocid (5 microm), but cells that were re-incubated in medium ...19948071190
[analysis of key enzyme activities involved in aspartate amino acid biosynthesis in streptococcus bovis].the first and the third steps in the aspartate biosynthesis pathway in streptococcus bovis are catalyzed by two different forms of aspartokinase and a single homoserine dehydrogenase, respectively. these enzymes can be separated by ammonium sulfate fractionation and gel filtration on sephadex g-200. the two aspartokinase isozymes differ in molecular weights and are subject to differential regulation. the aspartokinase system of s. bovis is characterized by the absence of specific negative allost ...19948117840
influence of yucca shidigera extract on ruminal ammonia concentrations and ruminal microorganisms.an extract of the desert plant yucca shidigera was assessed for its possible benefit in ruminal fermentation. the extract bound ammonia in aqueous solution when concentrations of ammonia were low (up to 0.4 mm) and when the extract was added at a high concentration to the sample (20%, vol/vol). the apparent ammonia-binding capability was retained after autoclaving and was decreased slightly following dialysis. acid-precipitated extract was inactive. no evidence of substantial ammonia binding was ...19948031077
regulation of two aspartokinase isozymes in streptococcus bovis.streptococcus bovis has been found to contain two distinct aspartokinases that can be separated by gel filtration chromatography. one of these isozymes elutes on sephadex g-200 gel filtration at a molecular weight greater than 250,000. the molecular weight of the other isozyme is approximately 125,000. the earlier peak of aspartokinase activity is slightly inhibited by meso-diaminopimelate, while the second peak is sensitive to inhibition by lysine. the latter aspartokinase is not formed when th ...19948012909
[rumen bacterial metabolism as affected by extracellular redox potential].the redox potential (eh) in the digestive tract of ruminants varies mostly within the ranges from -300 to +200 mv, in the rumen medium: from -130 to -200 mv. eh and ph changes are of a linear character. enhanced fermentation moves eh towards negative values and improves growth of microorganisms. eh values become even more negative as affected by na2s, naoh, cysteine and nahso3. addition of picrate, sodium nitrate, hydrochloric acid, copper sulphate, gaseous oxygen and particularly heavy metals ( ...19947974836
an unusual outbreak of streptococcus bovis septicaemia in racing pigeons (columba livia).in december 1991, an outbreak of streptococcus bovis septicaemia occurred in a belgian pigeon loft where 25 male and 25 female racing pigeons were housed. the main clinical signs included inability to fly and poor breeding results. none of the female pigeons and only one male pigeon was able to fly. nine affected pigeons were necropsied. histologically a tenosynovitis of the tendon of the deep pectoral muscle was observed in most of them and s bovis was isolated from the canalis triosseus or the ...19948135007
the adherence of three streptococcus bovis strains to cells of rumen epithelium primoculture under various conditions.three streptococcus bovis strains were tested in biotype assay and examined for the adherence to cells of rumen epithelium primoculture. the adherence pattern of ruminal streptococci in phosphate buffered saline at ph values ranging from 4.1 to 8.5 was determined. our isolates of streptococcus bovis strains adhered best at ph 7.0-7.3. to characterize the adhesive determinants, the bacterial cells were exposed to various treatments. protease treatment dramatically decreased the adherence of all s ...19947778984
streptococcus bovis infections in pigeons: virulence of different serotypes.in a first experiment, the relative virulence for pigeons of 5 strains of s. bovis was assessed by experimental inoculations. two s. bovis serotype 1 strains, one serotype 2 strain and two serotype 3 strains were examined. one of the serotype 1 strains and the serotype 2 strain were isolated from pigeons that died from septicaemia. the other strains were isolated from cloaca samples of healthy pigeons. for each strain, 10-20 pigeons were intravenously inoculated with 1 x 10(9) cfu. morbidity aft ...19947801532
positive inotropic effect of streptococcus faecalis in isolated cardiac muscle.infective endocarditis is caused by bacterial colonization of the endocardium. because endocardium modulates mechanical performance of subjacent myocardium, we studied acute effects of bacteria on isolated cardiac muscle and on the functional role of the endocardium. bacteria, grown in broth at 37 degrees c, were added at increasing concentrations (10(2) to 10(6) bacteria/ml) to cat papillary muscles in krebs-ringer solution (1.25 mm ca2+, 35 degrees c). the endocardial surface was damaged by ex ...19947810743
[regulation of microbiologic processes in the rumen of ruminants by bacteriophages of streptococcus bovis].influence of bacteriophages of streptococcus bovis on microbial activity in the rumen was investigated in experiments on cows. main elements of the mechanism of bacteriophage action on the microflora have been detected. the daily feeding with bacteriophages results in productive infection of sensitive to them rumen bacteria and is associated with increasing bacteriophage concentration in the rumen contents, lower number and activity of amylolytic bacteria, higher cellolytic activity and increase ...19947845249
spontaneous peritonitis caused by streptococcus bovis. 19947876523
[pulmonary valve endocarditis caused by streptococcus bovis].the authors report a case of isolated infective endocarditis of the pulmonary valve due to streptococcus bovis in a 62-year-old male presented with delayed fever of unknown origin. there were no predisposing factors such as i.v. drug abuse or congenital heart disease. the clinical course was complicated with an epidose of pulmonary emboli occurring after the onset of treatment. the patient responded to the appropriate antibiotics without requiring valve surgery. the association of streptococcus ...19947917382
[staphylococcus bovis infective endocarditis associated with gastric adenocarcinoma]. 19947983908
prevalence of streptococcus bovis in racing pigeons.the prevalence of s. bovis in the intestinal tract of healthy racing pigeons was determined. crop and cloaca swab samples obtained from 810 pigeons from 14 different lofts and from 122 pigeons that were presented for routine health control were examined for the presence of s. bovis. pooled faecal samples were also obtained from pigeons in 82 different pigeon lofts. s. bovis was isolated from crop or cloaca samples of approximately 40% of pigeons of all ages by direct culture and from 80% of the ...19947985358
[formate metabolism by lactate-producing and lactate-utilizing rumen bacteria].physiological concentrations [< 11 mm] of formate do not violate the metabolism of s. bovis and m. elsdenii. a significant inhibition is caused by concentrations of 22 and 44 mm. in this case the process of ammonia formation in s. bovis is inhibited more pronouncedly. peculiar effects of formate (11 mm) on ldh, fdh, aconitase, isocitrate dehydrogenase, fumarase, l-mdh and malic-enzyme have been stated. the changes show that it enhances assimilation of sugars fermented to lactate in s. bovis, in ...19947879287
use of cephalexin-aztreonam-arabinose agar for selective isolation of enterococcus faecium.cephalexin-aztreonam-arabinose agar (caa), a new selective agar, was examined in comparison with nalidixic acid-colistin agar for the differentiation of enterococcus faecium from other enterococci and the ability to isolate the organism from feces. two hundred sixteen enterococcus isolates and a variety of gram-positive and gram-negative control strains were inoculated onto both media. all control strains of e. faecium were easily differentiated from enterococcus faecalis and enterococcus durans ...19947883889
immunity in pigeons against homologous and heterologous serotypes of streptococcus bovis after infection.groups of 20, 20 and 10 pigeons were intravenously inoculated with 1 x 10(9) cfu of a streptococcus bovis serotype 1, 2 and 3 strain, respectively. groups which received the highly virulent serotypes 1 or 2 strains were treated with antibiotics starting from 2 days post inoculation in order to prevent the development of clinical signs. pigeons inoculated with the low virulence s. bovis serotype 3 strain were left untreated. another group of 10 non-inoculated pigeons was used as challenge control ...19947886926
strep bovis endocarditis and colonic carcinoma. 19947960660
tumors of the colon increase the risk of developing streptococcus bovis endocarditis: case-control study. 19947986925
alternative strategies of 2-deoxyglucose resistance and low affinity glucose transport in the ruminal bacteria, streptococcus bovis and selenomonas ruminantium.streptococcus bovis and selenomonas ruminantium grew in the presence of the glucose analog, 2-deoxyglucose (2-dg), but the cells no longer had high affinity glucose transport. in s. bovis, 2-dg resistance was correlated with a decrease in phosphoenolpyruvate (pep)-dependent glucose phosphotransferase (pts) activity. the 2-dg-selected s. bovis cells relied solely upon a low affinity, facilitated diffusion mechanism of glucose transport and a 2-dg-resistant glucokinase (atp-dependent). the glucoki ...19947988891
[spondylodiscitis in subacute bacterial endocarditis. apropos of a case].occurring of spondylodiscitis as first manifestation of bacterial endocarditis in an uncommon condition. about fifty cases are reported in the literature. such association makes imperious the regular examination of the heart of patients with spondylodiscitis and spine radiography when a vertebral pain is found to be present in patients with bacterial endocarditis. an additional case is her by reported.19947596460
streptococcus bovis osteomyelitis of the ilium. 19947948534
cyclic amp in ruminal and other anaerobic bacteria.an examination of camp levels in predominant species of ruminal bacteria and other anaerobic bacteria was conducted. cellular camp concentrations of glucose-grown cultures of butyrivibrio fibrisolvens 49, prevotella ruminicola d31d, selenomonas ruminantium hd4 and d, megasphaera elsdenii b159, streptococcus bovis jb1, bacteroides thetaiotaomicron 5482, and clostridium acetobutylicum atcc 824 were determined at various times during growth by a competitive binding radioimmunoassay procedure. the r ...19947851742
[rumen bacteria degrading toxic mimosine and dihydroxypyridine compounds in china].four anaerobic strains were isolated from the rumen of the cattle which no specific toxic symptoms were seen when leucaena was fed in weizhou island beihai city, guangxi province, china. all of these strains (br-1, br-2, br-5 and br-7) possess degradative activities to toxic mimosine, 3-hydroxy-4 (1h) -pyridone (3, 4-dhp) and 2, 3-dihydroxypyridine (2, 3dhp) from leucaena, that were confirmed by analysis of hplc. pure and mixed cultures of these four strains in vitro degraded 44-59% of mimosine, ...19947871781
effect of extracellular lactate on growth of rumen lactate producers.the addition of na-lactate (50-150 mmol/l) to media with glucose had only marginal effect on the growth of rumen lactate-producing bacteria at ph between 6.5 and 5.8. butyrivibrio fibrisolvens was somewhat more sensitive to external lactate than streptococcus bovis, lactobacillus fermentum and selenomonas ruminantium. it can be concluded that rumen lactate producers, which proliferate at the onset of rumen lactic acidosis, are not influenced by the lactate accumulation, except some non-specific ...19947619002
a gene-targeting suicide vector for streptococcus bovis.a gene-targeting suicide vector for streptococcus bovis has been constructed using the escherichia coli/streptococcus shuttle plasmid, pmu1328, and a region derived from the broad host-range, gram-positive transposon, tn916. this suicide plasmid replicates autonomously in e. coli, but not in strep. bovis or strep. bovis tn916. under positive selection, the plasmid was shown to integrate into strep. bovis tn916 chromosomal dna at a frequency of 3 x 10(-8) cell-1 and was stably maintained for at l ...19957576523
identification of intracellular amylase activity in streptococcus bovis and streptococcus salivarius.the ruminal bacterium streptococcus bovis has been demonstrated to produce an extracellular amylase activity. we previously reported on the cloning of a gene from s. bovis encoding for what was initially believed to be the extracellular amylase. dna sequence analyses indicated that the amylase produced by the cloned gene did not match the n-terminus amino acid sequence of the purified extracellular amylase and contained no apparent leader sequence for secretion. analyses of crude extracts demons ...19957532504
streptococcus sanguis bacteremia and colorectal cancer: a case report.streptococcus sanguis, usually considered a nonpathogen of the oral cavity, was isolated from blood cultures from a patient who was subsequently found to have a cecal adenocarcinoma. further studies are needed to determine if streptococcus sanguis infections have diagnostic implications similar to those of streptococcus bovis.19957968799
intracellular survival and multiplication of virulent and less virulent strains of streptococcus bovis in pigeon macrophages.the intracellular fate of pigeon s. bovis strains ingested by macrophages was studied in vivo and in vitro. during in vivo experiments, histological and electron microscopical examinations demonstrated numerous cocci, which appeared to be actively multiplying, within splenic macrophages of pigeons experimentally inoculated with a highly virulent s. bovis serotype 1 strain. in pigeons inoculated with a low virulence serotype 3 strain, intracellular cocci were only occasionally observed. for in vi ...19957571367
antibiotic treatment of adults with infective endocarditis due to streptococci, enterococci, staphylococci, and hacek microorganisms. american heart association.to provide guidelines for the treatment of endocarditis in adults caused by the following microorganisms: viridans streptococci and other streptococci, enterococci, staphylococci, and fastidious gram-negative bacilli of the hacek group.19957474277
regulation of the lactose phosphotransferase system of streptococcus bovis by glucose: independence of inducer exclusion and expulsion mechanisms.streptococcus bovis had a diauxic pattern of glucose and lactose utilization, and both of these sugars were transported by the sugar phosphotransferase system (pts). lactose catabolism was inducible, and s. bovis used the tagatose pathway to ferment lactose. since a mutant that was deficient in glucose pts activity transported lactose as fast as the wild-type, it appeared that s. bovis has separate enzyme iis for glucose and lactose. the nonmetabolizable glucose analogue 2-deoxyglucose (2-dg) wa ...19957496538
carcinoma of the colon presenting as streptococcus sanguis bacteremia.bacteremia by streptococci that normally inhabit the gastrointestinal tract has been associated with colon carcinoma. such association is best known for streptococcus bovis, but has also been reported for other streptococci. in the present communication a patient is described who presented with streptococcus sanguis bacteremia and was subsequently found to suffer from an adenocarcinoma of the sigmoid. a possible association between bacteremia by commensal streptococci of both the upper and lower ...19957661185
identification of proteolytic rumen bacteria isolated from new zealand cattle.the protease activities of 212 strains of rumen bacteria isolated from new zealand cattle grazing pasture were measured. thirty-seven per cent of strains had activity greater than or equal to the proteolytic rumen bacterium prevotella ruminicola and 43 of these isolates were identified by morphology, carbon source utilization, gram stain, biochemical tests and fermentation end-product analysis. hierarchical cluster analysis showed that the strains formed four clusters: cluster a contained 26 str ...19957665388
differentiation of ruminal and human streptococcus bovis strains by dna homology and 16s rrna probes.streptococcus bovis is commonly present in the rumen, but strains of s. bovis have also occasionally been isolated from human blood or fecal samples. studies were undertaken with 16s rrna gene sequences and dna hybridizations to define the genetic relationships between these two groups of strains. ruminal strains were found to yield genomic dna restriction endonuclease digest patterns different from human strains when either the 16s rrna gene amplified from ruminal s. bovis strain jb1 or a conse ...19957580800
properties of two sugar phosphate phosphatases from streptococcus bovis and their potential involvement in inducer expulsion.streptococcus bovis possesses two sugar phosphate phosphatases (pases). pase i is a soluble enzyme that is inhibited by the membrane fractions from lactose-grown cells and is insensitive to activation by s46d hpr, an analog of hpr(ser-p) of the sugar phosphotransferase system. pase ii is a membrane-associated enzyme that can be activated 10-fold by s46d hpr, and it appears to play a role in inducer expulsion.19957592500
the in vitro uptake and metabolism of peptides and amino acids by five species of rumen bacteria.streptococcus bovis jb1, prevotella ruminicola b(1)4, selenomonas ruminantium z108, fibrobacter succinogenes s85 and anaerovibrio lipolytica 5s were incubated with either 14c-peptides (mol. wt, 200-1000) or 14c-amino acids to compare their rates of uptake and metabolism. in experiment 1, the bacteria were grown and incubated in a complex medium, but no uptake of 14c-labelled substrates occurred. when casein digest was omitted, uptake rates of 14c-peptides were different (p < 0.01) with each spec ...19957698948
[mycotic aneurysm of the posterior tibial artery and pseudo-phlebitis: contribution of color doppler ultrasonography].mycotic aneurysm of the posterior tibial artery and pseudophlebitis: role of color doppler sonography a case of a 78-year-old male patient presenting with endocarditis caused by streptococcus bovis and pseudophlebitis of the left lower limb is described. color doppler sonography ruled out thrombophlebitis and showed a large pulsatile mass of the posterior compartment of the leg due to a mycotic aneurysm of the posterior tibial artery. this aneurysm was confirmed by angiography and treated by sur ...19957745555
heterologous expression of an endoglucanase gene (enda) from the ruminal anaerobe ruminococcus flavefaciens 17 in streptococcus bovis and streptococcus sanguis.the heterologous expression of a cloned endoglucanase gene (enda) from the ruminal bacterium ruminococcus flavefaciens 17 was demonstrated in the streptococcus species s. bovis jb1 and s. sanguis dl1. the enda gene was introduced into s. bovis and s. sanguis using the escherichia coli/streptococcus shuttle vector pva838. expression of the gene was detected by clearing zones around the recombinant colonies on agar plates containing carboxymethylcellulose stained with congo red. s. bovis jb1 conta ...19957705607
[the isolation of streptococcus morbillorum from vaginal exudates].we have tested 195 vaginal secretions sent by gynecology service of this hospital between the years 1988-1990. we achieved positive culture for streptococci in 58 (30%) of these cultures, 26 (44.8%) corresponding to streptococcus morbillorum 9 (15.5%), to gardnerella vaginalis 5 (8.6%), to enterococcus faecalis-durans and to streptococcus agalactiae, 3 (5.1%) to streptococcus mitis and milleri 2 (3.4%), to streptococcus bovis and cremoris, and 1 (1.7%) to streptococcus salivarius, equinus and sa ...19957716323
cellodextrin efflux by the cellulolytic ruminal bacterium fibrobacter succinogenes and its potential role in the growth of nonadherent bacteria.when glucose or cellobiose was provided as an energy source for fibrobacter succinogenes, there was a transient accumulation (as much as 0.4 mm hexose equivalent) of cellobiose or cellotriose, respectively, in the growth medium. nongrowing cell suspensions converted cellobiose to cellotriose and longer-chain cellodextrins, and in this case the total cellodextrin concentration was as much as 20 mm (hexose equivalent). because cell extracts of glucose- or cellobiose-grown cells cleaved cellobioise ...19957646013
treatment of streptococcal endocarditis with a single daily dose of ceftriaxone and netilmicin for 14 days: a prospective multicenter study.a 2-week course of ceftriaxone (2 g) plus netilmicin (4 mg/kg), administered as one short daily iv infusion, was evaluated for the treatment of streptococcal endocarditis in an open multicenter study. of the 52 patients, 31 were infected with viridans streptococci, 18 with streptococcus bovis, two with gemella morbillorum, and one with group c streptococcus; 48 patients were assessable. infection was cured in 42 cases, 35 treated medically and seven treated both medically and surgically. five pa ...19958749624
location of the alpha-amylase gene in rumen streptococcus bovis strains distinguished by unstable amylase activity.genetic stability of amylase activity after serial subcultivation experiments with amylolytic ruminal streptococcus bovis strains was investigated. two strains amy+ and amy- were obtained. loss of amylase activity connected with the loss of plasmid dna was not found in these strains. the presence of the gene responsible for the amylase activity in the chromosome of these strains was revealed by hybridization of the alpha-amylase gene on pjk108 against chromosomal dna of s. bovis and bacillus sub ...19958851562
evaluation of the automicrobic system for the identification of streptococcus mutans.the performance of the automicrobic system with the vitek gram-positive identification card (biomérieux, france) in identifying strains of streptococcus mutans was studied. of 160 strains assayed, 72.5% were confirmed to be streptococcus mutans; the remainder were identified as other species of streptocci (streptococcus bovis, streptococcus uberis, streptococcus anginosus, streptococcus sanguis i and ii, streptococcus intermedius, and streptococcus constellatus).19958681990
infective endocarditis due to streptococcus bovis. a report of 53 cases.fifty-three patients (42 men; 11 women) with streptococcus bovis infective endocarditis attended a tertiary cardiology hospital between 1980 and 1991, and constituted 11% of the total number of infective endocarditis cases hospitalized there during that period. the mean age was 59 +/- 15 years; 15 had previously suffered valvular disease (12) or had a valvular prosthesis (3); one patient had had a previous infective endocarditis. the infective episode involved the aortic valve in 26 patients, bo ...19958682035
[neurologic complications of infectious endocarditis].ten patients with neurological complications (nc) among a retrospective series of 53 consecutive cases of infective endocarditis were studied: 6 males and 4 females, mean age 60 years. nc were present at admission in 6 cases: 2 generalized seizures, 4 strokes, associated with 2 meningeal syndromes. four cases of nc occurred during hospitalization: 1 stroke with come, 1 transient ischaemic attack, 2 generalized seizures. blood cultures were positive in 7 cases: 6 group d streptococci, 1 gram nega ...19958597340
[streptococcus bovis in a surgical wound and a colonic neoplasm].the association of colorectal carcinoma and septicemia or endocarditis by streptococcus bovis is well known. nonetheless, other localizations of infection by streptococcus bovis have not been associated with colorectal carcinoma. the case of association of colon neoplasm with infection by streptococcus bovis localized in the surgical wound of resection of a prostate adenoma by the transvesical route carried out four months previously is presented. possible intraoperative bacteremia colonizing th ...19958521226
streptococcus bovis-infected total hip arthroplasty.the case of a 51-year-old man who underwent a total hip arthroplasty following failed ao screw fixation of a subcapital femoral neck fracture is reported. infection of the prosthesis with streptococcus bovis type 1 followed a febrile illness. further investigation revealed an occult premalignant polyp in the proximal colon. colonic neoplasia and s. bovis bacteremia are associated with endocarditis; however, s. bovis is a rare pathogen infecting joint prostheses and should raise the possibility o ...19958523020
[streptococcus bovis as the cause of combined endocarditis of the aortic, mitral and tricuspid valves].a case of combined endocarditis of the aortic-, mitral- and tricuspid valves in an otherwise healthy cattle farmer is described. the infective agent was streptococcus bovis. the relation between s. bovis infection and endocarditis is discussed.19958545920
rumen fermentation and metabolic profile in conventional and gnotobiotic lambs.observations were carried out of actual acidity, volatile fatty acid (vfa) concentrations, enzyme activity in the rumen, total protein, urea, total lipid and glucose in the serum of conventional (cl) and gnotobiotic lambs (gl) in the period of milk nutrition. the inoculum of gnotobiotic lambs contained streptococcus bovis, prevoxella ruminicola, butyrivibrio fibrisolvens and selenomonas ruminantium at a concentration of 1.10(6) each. throughout the observation period the ph of the rumen contents ...19958585797
spontaneous peritonitis caused by streptococcus bovis: search for colonic neoplasia. 19958648071
survey of urease activity in ruminal bacteria isolated from domestic and wild ruminants.a total of 909 strains, including selenomonas ruminantium, lactobacillus sp., enterococcus sp. and staphylococcus sp., from the rumen of 104 domestic and wild ruminants was used in tests for urease activity. tests showed that 56.7% of s. ruminantium strains and 18.5% of lactobacilli manifested medium urease activity with mean values of 14.4 +/- 2.5 and 13.85 +/- 0.25 nkat ml-1, respectively. most of the enterococcus faecium (62.2%) and all of the e. faecalis isolates expressed urease activity wi ...19958569526
[sepsis due to streptococcus bovis]. 19968767881
isolated tricuspid valve endocarditis due to streptococcus bovis. 19968824995
the effect of amino nitrogen on the energetics of ruminal bacteria and its impact on energy spilling.the predominant ruminal bacteria that were obtained from a 10(8) dilution of ruminal fluid could be maintained as a mixed population for long periods as long as the bacteria were provided with a complex mixture of carbohydrates. growth of predominant ruminal bacteria in carbohydrate-limited, ammonia-excess, continuous cultures (0.07/h) had a low requirement for maintenance energy, but the nongrowth energy dissipation of ammonia-limited, carbohydrate-excess, predominant ruminal bacteria was appro ...19968872717
highly expressed human sialyl lewis antigen on cell surface of streptococcus gallolyticus. 19968602016
comparison of infective endocarditis in patients with and without previously recognized heart disease.in a consecutive series of patients with infective endocarditis, we compared the charts of 123 nonaddicted patients without previously known heart disease with those of 174 patients with native valve disease. the 2 groups were similar in age, sex, clinical findings, and mortality rates, but infective endocarditis was more often located on the aortic valve, more often due to streptococcus bovis and enterococci in patients without previously known heart disease.19968644676
breakdown of peptides from a casein hydrolysate by rumen bacteria. simultaneous study of enzyme activities and physicochemical parameters.the breakdown of a pancreatic hydrolysate of casein (tryptone) by an inoculum of ruminal mixed bacteria was studied in vitro. peptides were degradated at 33% after 5 h. the dipeptidyl aminopeptidase type 1 (dap-1), exoaminopeptidase and leucine aminopeptidase (lap) like activities were measured using, respectively, gly-arg-mna, ala-pna and leu-pna as substrates. while the total proteolytic activity remained stable throughout peptide breakdown, the dap-1 already present at the beginning of fermen ...19968987097
in vitro activity of quinupristin/dalfopristin (rp 59500) against a large collection of infrequently isolated or tested species.quinupristin/dalfopristin (rp 59500, synercid) is a parenteral streptogramin combination antimicrobial that possesses a synergistic and often bactericidal action against many grampositive species. in this study, a collection of 1270 uncommonly isolated or tested strains were evaluated for susceptibility to quinupristin/dalfopristin using agar dilution minimum inhibitory concentration (mic) methods described in the national committe for clinical laboratory standards. the greatest antimicrobial ac ...19968902412
[serum bactericidal rate. methodological proposal to predict the efficacy of antimicrobial therapy].several in vitro techniques have been developed, which are able to quantify the bactericidal activity of a determined antibacterial drug against an infective agent. the serum bactericidal rate (sbr) is proposed as a complementary technique for the determination of the "killing curve", the serum bactericidal test or the minimal bactericidal concentration. sbr takes into account the most advantageous features of both of them. sbr is based on quantifying at different times the survival of the bacte ...19968935561
back pain in the older patient. 19968882127
streptococcus bovis endocarditis and vertebral osteomyelitis. 19968886820
effects of a strain of saccharomyces cerevisiae (levucell sc1), a microbial additive for ruminants, on lactate metabolism in vitro.the effect of levucell sc, a strain of saccharomyces cerevisiae marked as a feed additive for ruminants, was investigated in vitro on lactate metabolism by the ruminal bacteria streptococcus bovis and megasphaera elsdenii. the coculture between 10(7) live cells x ml(-1) of sc and a streptococcus bovis strain in the presence of glucose reduced lactate production by the bacterial strain. live yeast cells were able to compete with streptococcus bovis for glucose utilization in strictly anaerobic co ...19968864215
streptococcus bovis spontaneous bacterial peritonitis in patients with alcoholic cirrhosis.spontaneous bacterial peritonitis (sbp) is a frequent cause of decompensated alcoholic cirrhosis. the authors describe the first two cases caused by infection with streptococcus bovis. they suggest that this microorganism may be present in the intestinal flora of these patients more frequently than assumed. furthermore, the course of sbp due to s. bovis may be particularly severe.19968864683
transformation of streptococcus bovis protoplasts by plasmid dna.the transformation and subsequent regeneration of ruminal strain streptococcus bovis ao24/85 protoplasts by plasmid dna was studied. the best stabilizer for regeneration of protoplasted cells was 5% sucrose in the regeneration medium and in the agar plates. optimal concentration of polyethylene glycol 6000 in the transformation medium was 25% for both plasmids tested. addition of ca2+ and mg2+ ions (2.5 mmol l-1) to the transformation medium increased the proportion of regenerated cells. transfo ...19968936377
characterization of proteolytic activities of rumen bacterial isolates from forage-fed cattle.the proteolytic activities of eight strains of ruminal bacteria isolated from new zealand cattle were characterized with respect to their cellular location, response to proteinase inhibitors and hydrolysis of artificial proteinase substrates. the streptococcus bovis strains had predominantly cell-bound activity, which included a mixture of serine and cysteine-type proteinases which had high activity against leucine p-nitroanilide (lpna). the eubacterium strains had a mainly cell-associated activ ...19968939033
purification and properties of extracellular glucosyltransferase from streptococcus bovis.eight streptococcus bovis strains were classified into 3 types on the basis of isoelectric point (pi) and molecular mass (m(r)) of extracellular glucosyltransferase. strains atcc 9809, 35034 and 43143 produced glucosyltransferase of pi 3.7 and m(r) 165 kda; strains atcc 15351, 27960 and 33317 produced glucosyltransferase of pi 4.1 and m(r) 140 kda; strains atcc 43085 and 43144 did not produce any glucosyltransferase. the glucosyltransferase form s. bovis 9809 was purified by bio-gel hydroxyapati ...19968941763
use of fura-2/am to measure intracellular free calcium in selenomonas ruminantium.this paper describes a procedure for loading the acetoxymethyl ester of fura-2 (fura-2/am), and the subsequent measurement of the concentration of intracellular free ca2+ ([ca2+]i) in selenomonas ruminantium (s. ruminantium) using this technique. to ascertain the optimal loading conditions, the effect was examined on the loading of fura-2/am of ethylenediamine-tetraacetic acid (edta), lysozyme, pluronic f127 alone, or the simultaneous application of edta and pluronic f127. individual administrat ...19968944431
[bacteremia and endocarditis caused by streptococcus bovis in patients with alcoholic hepatopathy without evidence of colonic pathology].the association of streptococcus bovis bacteremia and endocarditis with colonic pathology, mainly neoplastic, is well known. its relationship with liver disease without evidence of gastrointestinal disease has been rarely described. to analyze the association between s. bovis infection and liver disease, positive blood cultures for this microorganism in hospitalized patients in the internal medicine and gastroenterology departments from december 1993 until october 1995, have been reviewed. three ...19968962774
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