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the antimicrobial activity and beta-lactamase stability of cefpirome, a new fourth-generation cephalosporin in comparison with other agents.the antimicrobial activity of cefpirome was compared with amoxycillin/clavulanic acid, ampicillin/sulbactam, cefuroxime, ceftazidime, gentamicin and amikacin against 743 non-duplicate clinical isolates. mic50 and mic90 showed that the antibiotic was active against both gram-negative and gram-positive organisms. cefpirome was highly active against most of the enterobacteriaceae, including indole-positive proteus spp., aeromonas spp. (mic < or = 1 mg/l) and salmonella spp. (mic < or = 0.5 mg/l). n ...19938335498
the efficacy of teicoplanin in the treatment of endocarditis caused by gram-positive bacteria.twenty-six patients with endocarditis caused by streptococcus viridans (n = 12), enterococcus faecalis (n = 5), streptococcus bovis (n = 2), staphylococcus aureus (n = 3) and staphylococcus epidermidis (n = 4) were treated with teicoplanin at a dose of 600 mg/day in patients with normal renal function (creatinine clearance 80-120 ml/min) and at a dose of 400 mg/day in patients with moderately impaired renal function (creatinine clearance < 75 ml/min). in nine patients valve replacements were per ...19938335503
identification and characterization of the proba operon of streptococcus bovis.a genomic dna library of the rumen bacterium streptococcus bovis was constructed in escherichia coli, and recombinant plasmids able to complement proa and prob mutations of the host were found. southern hybridization and restriction analysis showed that a 3.5-kb fragment of s. bovis dna contained two genes, organized in an operon and coding for enzymes functionally similar to the glutamyl phosphate reductase-glutamyl kinase enzyme complex that in e. coli catalyzes the first step of proline biosy ...19938382022
the efficacy and safety of piperacillin/tazobactam in the therapy of bacteraemia.the efficacy of the combination of piperacillin plus tazobactam was studied in 142 patients who had a bacteriologically proven bacteraemia. seventy-three patients were bacteriologically evaluable and, of these, 55 had a follow-up blood culture; there was no follow-up culture in the other 18 patients, so that any cure could only be presumptive. of the 55 patients who were fully followed up, 32 received only piperacillin and tazobactam and 23 also received an aminoglycoside. overall, there were 67 ...19938383659
diversity of chromosomal genetic elements and gene identification in antibiotic-resistant strains of streptococcus pneumoniae and streptococcus bovis.antibiotic-resistant streptococcus pneumoniae (26 strains) and streptococcus bovis (28 strains), devoid of r plasmids, were examined for dna-dna homology to tn916 and tn3701. tn916-like structures were found in 17 s. pneumoniae and 21 s. bovis strains. tn916-modified structures were present in 6 s. pneumoniae and 2 s. bovis strains. two strains of each species carried elements having a tn3701-like composite structure. all these elements were chromosome-borne. no chromosomal elements were detecte ...19938385787
adherence of glucan-positive and glucan-negative strains of streptococcus bovis to human epithelial cells.adherence to buccal epithelial cells (bec) and the role played in the binding by lipoteichoic acid (lta) and other superficial components have been studied in reference and clinical strains of streptococcus bovis either glucan-positive biotype i or glucan-negative biotype ii. to avoid the synthesis of glucan by biotype i strains, adherence was studied in bacteria grown in todd-hewitt broth, a sucrose deficient medium. both biotypes were shown to bind to bec and clinical isolates, irrespective of ...19938392108
characterization of bacteriocins from enterococcus faecium with activity against listeria monocytogenes.laboratory cultures and environmental isolates of bacteria were screened for antagonism towards listeria monocytogenes using an agar spot test. seven of the 163 strains that were tested, one streptococcus bovis, one enterococcus casseliflavus, two e. avium and three e. faecium, consistently displayed antilisterial activity. cell-free, ph-neutralized supernatants prepared from the three e. faecium strains (jbl1061, jbl1083 and jbl1351) exhibited strong antilisterial activity against l. monocytoge ...19938398626
the glutamine cyclotransferase reaction of streptococcus bovis: a novel mechanism of deriving energy from non-oxidative and non-reductive deamination.streptococcus bovis deaminated glutamine by a mechanism that did not involve glutaminase. since pyroglutamate and ammonia were the only end-products, it appeared that glutamine deamination was catalyzed by a cyclotransferase reaction. stationary s. bovis cells had essentially no intracellular atp or membrane potential (delta psi), however, when they were provided with glutamine, intracellular atp and delta psi increased to 0.52 mm and 158 mv, respectively. when glutamine-energized cells were tre ...19938405935
streptococcus bovis does not selectively colonize colorectal cancer and polyps.the objective was to determine if streptococcus bovis selectively colonizes colorectal cancer and polyps. stools were submitted before colonoscopy; fluid and selected tissue biopsies obtained during colonoscopy were cultured for s. bovis. the setting was a large multispecialty clinic. outpatients undergoing colonoscopy for suspected colorectal cancer and polyps were the participants. forty studies on 35 patients were performed. one of 35 stools (2.9%) obtained before colonoscopy yielded s. bovis ...19938409294
scleral flap necrosis and infectious endophthalmitis after cataract surgery with a scleral tunnel incision.long scleral tunnel dissection techniques have been developed for cataract surgery incisions. these incisions reduce postoperative astigmatism and keratorefractive instability. if fashioned correctly, the internal lip of the incision produces a tight seal to the anterior chamber, permissible of sutureless surgery. the behavior of such a wound during intraocular infection is unknown.19938437821
significance of streptococcus bovis endocarditis. 19938442633
in vitro activity and beta-lactamase stability of fk-037, a parenteral cephalosporin.the in vitro activity of fk-037, 5-amino-2-[[(6r, 7r)-7-[[(z)-2-(2-amino-4-thiazolyl)-2- methoxyimino) acetyl] amino]-2-carboxy-8-oxo-5-thia-1- azabicyclo[4.2.0]oct-2-en-3-yl]methyl]-1-(2-hydroxyethyl)-1h-pyrazoli um hydroxide, inner salt, sulfate (1:1), a new parenteral cephem, was compared with those of cefepime, ceftazidime, imipenem, and ciprofloxacin. fk-037 inhibited methicillin-susceptible staphylocci at < or = 4 micrograms/ml. of 98 isolates of homogenous methicillin-resistant staphyloco ...19938460925
[unusual presentation of streptococcus bovis endocarditis]. 19938474289
comparison of fluorescent gentamicin-thallous-carbonate and kf streptococcal agars to enumerate enterococci and fecal streptococci in meats.two selective and differential media were compared for their abilities to enumerate enterococci and fecal streptococci in pork, beef, and poultry products. counts obtained on kf streptococcal (kf) agar were compared with counts obtained on fluorescent gentamicin-thallous-carbonate (fgtc) agar. reactions of 13 known enterococcal species were also observed. all 13 species of enterococci as well as streptococcus bovis and streptococcus equinus grew equally well on fgtc agar. kf streptococcal medium ...19938481014
in vitro effects of a thiopeptide and monensin on ruminal fermentation of soluble carbohydrates.using a purified diet and a mixed culture of ruminal microorganisms, we studied the in vitro effects of a sulfur-containing peptide (a10255) and monensin on ruminal vfa and lactic acid concentrations. after 22 h of fermentation, total vfa concentration was greater in cultures with a10255 and monensin (2.5 ppm each) than in untreated controls (84.1, 91.9, and 65.2 mm for cultures with a10255, monensin, and control, respectively). the molar proportions of propionate were more than 40% greater, and ...19938486839
[the characteristics of the regulation of lysine biosynthesis in streptococcus bovis].the effect of threonine and methionine on the growth of and lysine synthesis in streptococcus bovis st. a024/85 was studied. the character and the degree of manifestation of growth and regulation effects of these amino acids depend on their contents in the fermentation medium. the target in the regulation of lysine biosynthesis is aspartate kinase, whose synthesis is controlled by threonine and methionine. lysine excretion was stimulated by the addition of a low concentration of dimethylsulfoxid ...19938488160
[occurrence of streptococci resistant to antibiotics in the rumen of calves].the occurrence and antibiotic-resistance spectrum of some strains of rumen streptococci were investigated in calves of four age categories in the milk diet period at 1-3, 4-7, 8-14 days of age and 6-8 weeks of age. more than 75% of the strains isolated from the calves of all age categories were resistant to more than one tested antibiotic (tetracycline, neomycin, erythromycin, streptomycin, chloramphenicol). the average number of streptococci isolated from the ingesta of calves during the period ...19938488631
suspicious mind: the association between streptococcus bovis endocarditis and carcinoma of the colon. 19938497446
[association of streptococcus bovis endocarditis and liver cirrhosis]. 19938511149
purification and characterization of the extracellular alpha-amylase from streptococcus bovis jb1.the extracellular alpha-amylase (1,4-alpha-d-glucanglucanohydrolase; ec 3.2.1.1) from maltose-grown streptococcus bovis jb1 was purified to apparent homogeneity by ion-exchange chromatography (mono q). the enzyme had an isoelectric point of 4.50 and an apparent molecular mass of 77,000 da, as estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. the enzyme was rich in acidic and hydrophobic amino acids. the 15-amino-acid nh2-terminal sequence was 40% homologous with the bacillu ...19938517735
[streptococcus bovis in a surgical wound and a colonic neoplasm].the association of colorectal carcinoma and septicemia or endocarditis by streptococcus bovis is well known. nonetheless, other localizations of infection by streptococcus bovis have not been associated with colorectal carcinoma. the case of association of colon neoplasm with infection by streptococcus bovis localized in the surgical wound of resection of a prostate adenoma by the transvesical route carried out four months previously is presented. possible intraoperative bacteremia colonizing th ...19958521226
streptococcus bovis-infected total hip arthroplasty.the case of a 51-year-old man who underwent a total hip arthroplasty following failed ao screw fixation of a subcapital femoral neck fracture is reported. infection of the prosthesis with streptococcus bovis type 1 followed a febrile illness. further investigation revealed an occult premalignant polyp in the proximal colon. colonic neoplasia and s. bovis bacteremia are associated with endocarditis; however, s. bovis is a rare pathogen infecting joint prostheses and should raise the possibility o ...19958523020
[streptococcus bovis as the cause of combined endocarditis of the aortic, mitral and tricuspid valves].a case of combined endocarditis of the aortic-, mitral- and tricuspid valves in an otherwise healthy cattle farmer is described. the infective agent was streptococcus bovis. the relation between s. bovis infection and endocarditis is discussed.19958545920
survey of urease activity in ruminal bacteria isolated from domestic and wild ruminants.a total of 909 strains, including selenomonas ruminantium, lactobacillus sp., enterococcus sp. and staphylococcus sp., from the rumen of 104 domestic and wild ruminants was used in tests for urease activity. tests showed that 56.7% of s. ruminantium strains and 18.5% of lactobacilli manifested medium urease activity with mean values of 14.4 +/- 2.5 and 13.85 +/- 0.25 nkat ml-1, respectively. most of the enterococcus faecium (62.2%) and all of the e. faecalis isolates expressed urease activity wi ...19958569526
rumen fermentation and metabolic profile in conventional and gnotobiotic lambs.observations were carried out of actual acidity, volatile fatty acid (vfa) concentrations, enzyme activity in the rumen, total protein, urea, total lipid and glucose in the serum of conventional (cl) and gnotobiotic lambs (gl) in the period of milk nutrition. the inoculum of gnotobiotic lambs contained streptococcus bovis, prevoxella ruminicola, butyrivibrio fibrisolvens and selenomonas ruminantium at a concentration of 1.10(6) each. throughout the observation period the ph of the rumen contents ...19958585797
[neurologic complications of infectious endocarditis].ten patients with neurological complications (nc) among a retrospective series of 53 consecutive cases of infective endocarditis were studied: 6 males and 4 females, mean age 60 years. nc were present at admission in 6 cases: 2 generalized seizures, 4 strokes, associated with 2 meningeal syndromes. four cases of nc occurred during hospitalization: 1 stroke with come, 1 transient ischaemic attack, 2 generalized seizures. blood cultures were positive in 7 cases: 6 group d streptococci, 1 gram nega ...19958597340
highly expressed human sialyl lewis antigen on cell surface of streptococcus gallolyticus. 19968602016
comparison of infective endocarditis in patients with and without previously recognized heart disease.in a consecutive series of patients with infective endocarditis, we compared the charts of 123 nonaddicted patients without previously known heart disease with those of 174 patients with native valve disease. the 2 groups were similar in age, sex, clinical findings, and mortality rates, but infective endocarditis was more often located on the aortic valve, more often due to streptococcus bovis and enterococci in patients without previously known heart disease.19968644676
spontaneous peritonitis caused by streptococcus bovis: search for colonic neoplasia. 19958648071
evaluation of the automicrobic system for the identification of streptococcus mutans.the performance of the automicrobic system with the vitek gram-positive identification card (biomérieux, france) in identifying strains of streptococcus mutans was studied. of 160 strains assayed, 72.5% were confirmed to be streptococcus mutans; the remainder were identified as other species of streptocci (streptococcus bovis, streptococcus uberis, streptococcus anginosus, streptococcus sanguis i and ii, streptococcus intermedius, and streptococcus constellatus).19958681990
infective endocarditis due to streptococcus bovis. a report of 53 cases.fifty-three patients (42 men; 11 women) with streptococcus bovis infective endocarditis attended a tertiary cardiology hospital between 1980 and 1991, and constituted 11% of the total number of infective endocarditis cases hospitalized there during that period. the mean age was 59 +/- 15 years; 15 had previously suffered valvular disease (12) or had a valvular prosthesis (3); one patient had had a previous infective endocarditis. the infective episode involved the aortic valve in 26 patients, bo ...19958682035
streptococcus bovis endocarditis and vertebral osteomyelitis.streptococcus bovis recovered from blood cultures may represent bacteremia alone, bacteremia from an extravascular source, or endocarditis. patients with s. bovis endocarditis frequently have a gastrointestinal focus. s. bovis has been associated with many gastrointestinal diseases and in particular with colon carcinoma. s. bovis endocarditis may be complicated by embolic events or osteomyelitis. vertebral osteomyelitis is a rare complication of s. bovis endocarditis. we report the third known c ...19968682689
purification and characterization of a malic enzyme from the ruminal bacterium streptococcus bovis atcc 15352 and cloning and sequencing of its gene.malic enzyme (ec 1.1.1.39), which catalyzes l-malate oxidative decarboxylation and pyruvate reductive carboxylation, was purified to homogeneity from streptococcus bovis atcc 15352, and properties of this enzyme were determined. the 2.9-kb fragment containing the malic enzyme gene was cloned, and the sequence was determined and analyzed. the enzymatic properties of the s. bovis malic enzyme were almost identical to those of other malic enzymes previously reported. however, we found that the s. b ...19968702261
treatment of streptococcal endocarditis with a single daily dose of ceftriaxone and netilmicin for 14 days: a prospective multicenter study.a 2-week course of ceftriaxone (2 g) plus netilmicin (4 mg/kg), administered as one short daily iv infusion, was evaluated for the treatment of streptococcal endocarditis in an open multicenter study. of the 52 patients, 31 were infected with viridans streptococci, 18 with streptococcus bovis, two with gemella morbillorum, and one with group c streptococcus; 48 patients were assessable. infection was cured in 42 cases, 35 treated medically and seven treated both medically and surgically. five pa ...19958749624
rapid identification of streptococcus and enterococcus species using diffuse reflectance-absorbance fourier transform infrared spectroscopy and artificial neural networks.diffuse reflectance-absorbance fourier transform infrared spectroscopy (ft-ir) was used to analyse 19 hospital isolates which had been identified by conventional means to one enterococcus faecalis, e. faecium, streptococcus bovis, s. mitis, s. pneumoniae, or s. pyogenes. principal components analysis of the ft-ir spectra showed that this 'unsupervised' learning method failed to form six separable clusters (one of each species) and thus could not be used to identify these bacteria base on their f ...19968764485
[sepsis due to streptococcus bovis]. 19968767881
catabolite regulation in a diauxic strain and a nondiauxic strain of streptococcus bovis.streptococcus bovis jb1 utilized glucose preferentially to lactose and grew diauxically, but s. bovis 581axy2 grew nondiauxically and used glucose preferentially only when the glucose concentration was very high (greater than 5 mm). as little as 0.1 mm glucose completely inhibited the lactose transport of jb1. the lactose transport system of 581axy2 was at least tenfold less sensitive to glucose, and 1 mm glucose caused only a 50% inhibition of lactose transport. both strains had phosphotransfer ...19968824165
isolated tricuspid valve endocarditis due to streptococcus bovis. 19968824995
location of the alpha-amylase gene in rumen streptococcus bovis strains distinguished by unstable amylase activity.genetic stability of amylase activity after serial subcultivation experiments with amylolytic ruminal streptococcus bovis strains was investigated. two strains amy+ and amy- were obtained. loss of amylase activity connected with the loss of plasmid dna was not found in these strains. the presence of the gene responsible for the amylase activity in the chromosome of these strains was revealed by hybridization of the alpha-amylase gene on pjk108 against chromosomal dna of s. bovis and bacillus sub ...19958851562
effects of a strain of saccharomyces cerevisiae (levucell sc1), a microbial additive for ruminants, on lactate metabolism in vitro.the effect of levucell sc, a strain of saccharomyces cerevisiae marked as a feed additive for ruminants, was investigated in vitro on lactate metabolism by the ruminal bacteria streptococcus bovis and megasphaera elsdenii. the coculture between 10(7) live cells x ml(-1) of sc and a streptococcus bovis strain in the presence of glucose reduced lactate production by the bacterial strain. live yeast cells were able to compete with streptococcus bovis for glucose utilization in strictly anaerobic co ...19968864215
streptococcus bovis spontaneous bacterial peritonitis in patients with alcoholic cirrhosis.spontaneous bacterial peritonitis (sbp) is a frequent cause of decompensated alcoholic cirrhosis. the authors describe the first two cases caused by infection with streptococcus bovis. they suggest that this microorganism may be present in the intestinal flora of these patients more frequently than assumed. furthermore, the course of sbp due to s. bovis may be particularly severe.19968864683
inducer expulsion is not a determinant of diauxic growth in streptococcus bovis.when streptococcus bovis jb1 was repeatedly transferred in a medium that contained the non-metabolizable glucose analog, 2-deoxyglucose, it lost its phosphotransferase system (pts) for glucose but was still able to take up glucose via a facilitated diffusion mechanism. the wild type (jb1) had an inducible enzyme ii lactose, but the mutant (jb1(2dg)) had a constitutive lactose pts. jb1(2dg) was no longer able to exclude lactose when it was provided with glucose, but it retained its ability to exp ...19968867462
inducer expulsion and the occurrence of an hpr(ser-p)-activated sugar-phosphate phosphatase in enterococcus faecalis and streptococcus pyogenes.inducer expulsion, a phenomenon in which rapidly metabolizable sugars cause cytoplasmic dephosphorylation and efflux of pre-accumulated sugar-phosphates (sugar-p), has been documented for streptococcus pyogenes, streptococcus bovis, and lactococcus lactis, but not for other gram-positive bacteria. using intact cells and membrane vesicles, we show that enterococcus faecalis exhibits both inducer exclusion and inducer expulsion, and that the latter phenomenon is dependent on the metabolite-activat ...19968868433
the effect of amino nitrogen on the energetics of ruminal bacteria and its impact on energy spilling.the predominant ruminal bacteria that were obtained from a 10(8) dilution of ruminal fluid could be maintained as a mixed population for long periods as long as the bacteria were provided with a complex mixture of carbohydrates. growth of predominant ruminal bacteria in carbohydrate-limited, ammonia-excess, continuous cultures (0.07/h) had a low requirement for maintenance energy, but the nongrowth energy dissipation of ammonia-limited, carbohydrate-excess, predominant ruminal bacteria was appro ...19968872717
tricuspid valve endocarditis caused by streptococcus bovis. 19968879815
back pain in the older patient. 19968882127
streptococcus bovis endocarditis and vertebral osteomyelitis. 19968886820
in vitro activity of quinupristin/dalfopristin (rp 59500) against a large collection of infrequently isolated or tested species.quinupristin/dalfopristin (rp 59500, synercid) is a parenteral streptogramin combination antimicrobial that possesses a synergistic and often bactericidal action against many grampositive species. in this study, a collection of 1270 uncommonly isolated or tested strains were evaluated for susceptibility to quinupristin/dalfopristin using agar dilution minimum inhibitory concentration (mic) methods described in the national committe for clinical laboratory standards. the greatest antimicrobial ac ...19968902412
[serum bactericidal rate. methodological proposal to predict the efficacy of antimicrobial therapy].several in vitro techniques have been developed, which are able to quantify the bactericidal activity of a determined antibacterial drug against an infective agent. the serum bactericidal rate (sbr) is proposed as a complementary technique for the determination of the "killing curve", the serum bactericidal test or the minimal bactericidal concentration. sbr takes into account the most advantageous features of both of them. sbr is based on quantifying at different times the survival of the bacte ...19968935561
transformation of streptococcus bovis protoplasts by plasmid dna.the transformation and subsequent regeneration of ruminal strain streptococcus bovis ao24/85 protoplasts by plasmid dna was studied. the best stabilizer for regeneration of protoplasted cells was 5% sucrose in the regeneration medium and in the agar plates. optimal concentration of polyethylene glycol 6000 in the transformation medium was 25% for both plasmids tested. addition of ca2+ and mg2+ ions (2.5 mmol l-1) to the transformation medium increased the proportion of regenerated cells. transfo ...19968936377
characterization of proteolytic activities of rumen bacterial isolates from forage-fed cattle.the proteolytic activities of eight strains of ruminal bacteria isolated from new zealand cattle were characterized with respect to their cellular location, response to proteinase inhibitors and hydrolysis of artificial proteinase substrates. the streptococcus bovis strains had predominantly cell-bound activity, which included a mixture of serine and cysteine-type proteinases which had high activity against leucine p-nitroanilide (lpna). the eubacterium strains had a mainly cell-associated activ ...19968939033
purification and properties of extracellular glucosyltransferase from streptococcus bovis.eight streptococcus bovis strains were classified into 3 types on the basis of isoelectric point (pi) and molecular mass (m(r)) of extracellular glucosyltransferase. strains atcc 9809, 35034 and 43143 produced glucosyltransferase of pi 3.7 and m(r) 165 kda; strains atcc 15351, 27960 and 33317 produced glucosyltransferase of pi 4.1 and m(r) 140 kda; strains atcc 43085 and 43144 did not produce any glucosyltransferase. the glucosyltransferase form s. bovis 9809 was purified by bio-gel hydroxyapati ...19968941763
use of fura-2/am to measure intracellular free calcium in selenomonas ruminantium.this paper describes a procedure for loading the acetoxymethyl ester of fura-2 (fura-2/am), and the subsequent measurement of the concentration of intracellular free ca2+ ([ca2+]i) in selenomonas ruminantium (s. ruminantium) using this technique. to ascertain the optimal loading conditions, the effect was examined on the loading of fura-2/am of ethylenediamine-tetraacetic acid (edta), lysozyme, pluronic f127 alone, or the simultaneous application of edta and pluronic f127. individual administrat ...19968944431
[bacteremia and endocarditis caused by streptococcus bovis in patients with alcoholic hepatopathy without evidence of colonic pathology].the association of streptococcus bovis bacteremia and endocarditis with colonic pathology, mainly neoplastic, is well known. its relationship with liver disease without evidence of gastrointestinal disease has been rarely described. to analyze the association between s. bovis infection and liver disease, positive blood cultures for this microorganism in hospitalized patients in the internal medicine and gastroenterology departments from december 1993 until october 1995, have been reviewed. three ...19968962774
[bilateral epidural psoas abscess simulating bilateral proximal diabetic plexopathy].we describe a diabetic woman with an epidural abscess and associated bilateral psoas abscesses which simulated the clinical course of diabetic bilateral proximal plexopathy. we emphasize three aspects: 1) the rarity of associated epidural abscess and bilateral psoas abscesses, 2) the rarity of the germ isolated (streptococcus bovis), and 3) the diagnostic difficulties when both processes are associated, particularly in patients with an underlying disease that may simulate other common, clinical ...19968974430
emergence of vancomycin resistance in the genus streptococcus: characterization of a vanb transferable determinant in streptococcus bovis.streptococcus bovis nem760 was isolated from a stool swab collected on admission from a patient as surveillance for vancomycin-resistant enterococci. strain nem760 was identified as s. bovis by conventional biochemical methods and partial sequence analysis of its 16s rrna. this strain was resistant to a low level of vancomycin (mic, 64 micrograms/ml) but was susceptible to teicoplanin (mic, 1 micrograms/ml), and vancomycin induced resistance to both glycopeptides. the presence of a vanb-related ...19978980749
breakdown of peptides from a casein hydrolysate by rumen bacteria. simultaneous study of enzyme activities and physicochemical parameters.the breakdown of a pancreatic hydrolysate of casein (tryptone) by an inoculum of ruminal mixed bacteria was studied in vitro. peptides were degradated at 33% after 5 h. the dipeptidyl aminopeptidase type 1 (dap-1), exoaminopeptidase and leucine aminopeptidase (lap) like activities were measured using, respectively, gly-arg-mna, ala-pna and leu-pna as substrates. while the total proteolytic activity remained stable throughout peptide breakdown, the dap-1 already present at the beginning of fermen ...19968987097
secreted antigens as virulence associated markers in streptococcus bovis strains from pigeons.sds-page and western blot analysis were performed on the culture supernatant of 7 pigeon s. bovis reference strains belonging to the serotypes 1, 2, 3 and 5. the culture supernatant of highly virulent serotype 1, 2 and 5 strains contained four antigens that were absent in low virulent serotype 3 strains, notably a 185 kda minor protein band (a) and a triplet (t1) of 70 kda. the less virulent serotype 3 strains on the other hand contained protein triplets, that had a molecular mass of either 68 k ...19969008344
isolation and comparative analysis of diaminopimelate decarboxylase from streptococcus bovis and bacillus subtilis. 19979044297
[serologic study in patients with streptococcal infective endocarditis].infective endocarditis is a systemic disease in which there are a continuously antigenic stimulation of immunologic system. streptococcus is still the most frequent cause of infective endocarditis.19969082078
[biology of lysogenic strains of streptococcus bovis and virulent mutants of their temperate phages].three lysogenic streptococcal strains and four virulent mutants of temperate phages were studied. the bacterial strains proved to be close to the type strain of streptococcus bovis in their morphological, cultural, physiological, and biochemical properties. investigation into the interaction of the lysogenic cultures with virulent mutants of temperate phages showed that the culture age optimal for infecting was 3-5 h; intense lysis began in the second hour after infection and was virtually compl ...19969102549
polymicrobial brain abscess in a patient infected with human immunodeficiency virus.although intracranial mass lesions that occur as a result of infection have commonly been reported in patients infected with human immunodeficiency virus, polymicrobial pyogenic brain abscess has not been described in this setting. we report the first case of a patient with a polymicrobial brain abscess involving streptococcus bovis, fusobacterium necrophorum, peptostreptococcus, and group g streptococcus, and we review the relevant world literature.19979114198
cloning, sequence, and expression of the l-(+) lactate dehydrogenase of streptococcus bovis.the ldh gene encoding the fructose-1,6-diphosphate-dependent l-(+) lactate dehydrogenase from the ruminal bacterium streptococcus bovis was cloned and sequenced. a genomic library of s. bovis jb1 dna was constructed in lambda zap ii and screened by use of a heterologous probe derived from the cloned streptococcus mutans ldh gene. several clones were isolated that contained a common 2.9-kb fragment as determined by restriction analysis. nucleotide sequence analysis revealed a 987-bp open reading ...19979142744
isolated septic arthritis due to streptococcus bovis. 19979142824
ocular manifestations of disseminated intravascular coagulation in a patient with the acquired immunodeficiency syndrome. 19979152141
activity of h(+)-atpase in ruminal bacteria with special reference to acid tolerance.batch culture experiments showed that permeabilized cells and membranes of ruminococcus albus and fibrobacter succinogenes, acid-intolerant celluloytic bacteria, have only one-fourth to one-fifth as much h(+)-atpase as megasphaera elsdenii and streptococcus bovis, which are relatively acid tolerant. even in the cells grown in continuous culture at ph 7.0, the acid-intolerant bacteria contained less than half as much h(+)-atpase as the acid-tolerant bacteria. the amounts of h(+)-atpase in the aci ...19979172333
characterization of the l-malate permease gene (maep) of streptococcus bovis atcc 15352.a gene which was shown to be cotranscribed with the nad+-dependent malic enzyme gene (maee) of streptococcus bovis atcc 15352 was revealed to encode l-malate-specific permease (maep), which showed high activity at low phs (ph 5.1 to 5.9). maep was strongly inhibited by the ionophores nigericin and valinomycin.19979190827
fatal streptococcus bovis sepsis in an infant on peritoneal dialysis. 19979203204
gene knockout of the intracellular amylase gene by homologous recombination in streptococcus bovis.streptococcus bovis expresses two different amylases, one intracellular and the other secreted. a suicide vector containing part of the intracellular alpha-amylase gene from streptococcus bovis wi-1 was recombined into the s. bovis wi-1 chromosome to disrupt the endogenous gene. recombination was demonstrated by southern blot, and zymogram analysis confirmed the loss of the intracellular amylase. amylase activity in cell-free extracts of the recombinant grown in the presence of 1% starch was onl ...19979236293
liver biopsy as a cause of streptococcus bovis peritonitis. 19979252867
lateral neck abscess caused by streptococcus bovis in a patient with undiagnosed colon cancer.bacteraemia or endocarditis caused by streptococcus bovis, a non-enterococcal group d streptococcus, is a very common finding in patients suffering from intestinal tumours or other colon diseases. a case of a patient with a laternal neck abscess caused by s. bovis is reported. during the treatment the patient was found to have colonic malignant lesions. the problems in the appropriate diagnosis and the possible correlation between these two clinical entities are presented and discussed.19979282212
[infection by strongyloides stercoralis in the county of safor, spain].strongyloides stercoralis is an endemic nematode in tropical and subtropical regions, but almost unknown in spain.19979289550
isolated tricuspid valve endocarditis in nonaddicted patients: a diagnostic challenge.isolated native nonrheumatic tricuspid valve endocarditis rarely is described in the absence of intravenous drug use, intracardiac catheters, or cardiac anomalies. we diagnosed tricuspid valve endocarditis in two elderly nonaddicted patients with recurrent pulmonary infiltrates, anemia, and microscopic hematuria that occurred during several months and was caused by gemella morbillorum and candida glabrata, respectively. we have reviewed 27 other cases of nonaddicted patients with tricuspid valve ...19979298048
comparative in-vitro activities of quinupristin-dalfopristin against gram-positive bloodstream isolates.the in-vitro activity of quinupristin-dalfopristin was compared with those of vancomycin, teicoplanin, erythromycin, clarithromycin, rifampicin, imipenem, meropenem, ciprofloxacin and sparfloxacin against 414 bloodstream isolates of gram-positive cocci. quinupristin-dalfopristin inhibited strains of streptococcus pyogenes and streptococcus agalactiae at 0.12 mg/l, methicillin- and/or erythromycin-resistant staphylococcus aureus and staphylococcus epidermidis at 0.5 mg/l, staphylococcus haemolyti ...19979301986
perforated cecal adenocarcinoma presenting as a thigh abscess.complicated colorectal carcinoma has several symptoms, the most common being bleeding and obstruction. occasionally it will cause perforation, which carries a worse prognosis. we report a case of perforated adenocarcinoma of the cecum that presented as an abscess of the thigh. we also present a review of the literature on this subject.19979305311
isolation and overexpression of a gene encoding an extracellular beta-(1,3-1,4)-glucanase from streptococcus bovis jb1.streptococcus bovis jb1 was found to produce a 25-kda extracellular enzyme active against beta-(1,3-1,4)-glucans. a gene was isolated encoding a specific beta-(1,3-1,4)-glucanase that corresponds to this size and belongs to glycoside hydrolase family 16. a 4- to 10-fold increase in supernatant beta-glucanase activity was obtained when the cloned beta-glucanase gene was reintroduced into s. bovis jb1 by use of constructs based on the plasmid vector ptrw10 or pil253. the beta-(1,3-1,4)-glucanase g ...19979327538
biotype distribution of enterococci and group d streptococci recovered from clinical material. 19979331654
streptococcus infantarius sp. nov. related to streptococcus bovis and streptococcus equinus. 19979331678
use of the malthus conductance growth analyser to determine numbers of thermophilic streptococci on stainless steel.the use of the malthus conductance growth analyser for the detection of streptococcus bovis attached to stainless steel surface was evaluated. a comparison between the results from acridine orange epifluorescence direct counts, swab recovery viable count and conductance estimates of attached cell concentrations, based on calibrations for planktonic cells, showed that the conductance results were up to 2 log10 greater than the epifluorescence results and the swab counts. the growth rates of plank ...19979351213
streptococcus bovis infection of the central nervous system: report of two cases and review.streptococcus bovis is an uncommon cause of meningitis and subdural empyema. we report one case each of meningitis and subdural empyema in which s. bovis biotype ii was isolated from both the spinal fluid and blood. in one case, the organisms were seen on a gram-stained preparation of cerebrospinal fluid. the first patient presented with gastrointestinal symptoms of unknown etiology, was immunosuppressed, and recovered. the second patient presented with syncope, developed a subdural empyema, and ...19979356795
application of the extracellular alpha-amylase gene from streptococcus bovis 148 to construction of a secretion vector for yogurt starter strains.streptococcus thermophilus atcc 19258, lactobacillus delbrueckii subsp. bulgaricus t-11, and lactococcus lactis subsp. lactis il1403 were transformed with the alpha-amylase gene (amya) from streptococcus bovis 148 by using a wide host-range vector, and all the transformants secreted the alpha-amylase successfully. since the promoter and the secretion signal of the amya gene were functional in these strains, we constructed a secretion vector using the expression elements of amya. trials to secret ...19979361445
coronary embolism complicating aortic valve endocarditis: treatment with placement of an intracoronary stent.a 37-year-old man, who had received 3 weeks of antimicrobial therapy for aortic value endocarditis, presented with an acute anteroseptal wall myocardial infarction. coronary angiography demonstrated occlusion of the mid left anterior descending artery, thought to have been caused by embolization of a sterile vegetation. following failure of balloon dilation to achieve vessel patency, this was achieved by placement of an intracoronary stent.19979377827
[combination of colorectal tumor and streptococcus bovis endocarditis. apropos of 3 cases].the authors report about 3 cases of bovis endocarditis revealing a colic carcinoma. this morbidity confirms the need for routine digestive investigations in endocarditis due to group d streptococci and eventually a liver disease.19979378787
purification, characterization, and nucleotide sequence of an intracellular maltotriose-producing alpha-amylase from streptococcus bovis 148.an intracellular alpha-amylase from streptococcus bovis 148 was purified and characterized. the enzyme was induced by maltose and soluble starch and produced about 80% maltotriose from soluble starch. maltopentaose was hydrolyzed to maltotriose and maltose and maltohexaose was hydrolyzed mainly to maltotriose by the enzyme. maltotetraose, maltotriose, and maltose were not hydrolyzed. this intracellular enzyme was considered to be a maltotriose-producing enzyme. the enzymatic characteristics and ...19979406414
[streptococcus bovis bacteremia in a patient with alcoholic hepatopathy without colonic pathology]. 19979445550
[native valve infective endocarditis caused by streptococcus bovis. efficacy of short-term antibiotic therapy and usefulness of serial echocardiographic evaluation].we report a case of infective endocarditis on native valve, due to streptococcus bovis, treated successfully with short time antibiotic therapy (10 days versus minimum suggested treatment of two weeks) by using penicillin g together with streptomycin (six days), followed, by treatment with imipenem (four days) because of allergic reactions. diagnosis was simpler thanks to durack's new criteria that include positive echocardiographic findings (valvular vegetations) within major clinical criteria ...19979446156
extracellular proteins and virulence in streptococcus bovis isolates from pigeons.the association between virulence and the occurrence of the extracellular proteins a, t1, t2 and t3 in the culture supernatant of pigeon streptococcus bovis strains, was examined in experimental infection studies. fourteen groups of 10-17 pigeons were inoculated intravenously with 1 x 10(9) cfu of s. bovis strains that belonged to the phenotypes a + t1, a - t1, a + t2, a - t2, a + t3 and a - t3, respectively. the overall postinoculation morbidity in the phenotype groups was 85%, 87%, 70%, 5%, 10 ...19979460197
the ability of 2-deoxyglucose to promote the lysis of streptococcus bovis jb1 via a mechanism involving cell wall stability.the non-metabolizable glucose analog, 2-deoxyglucose (2-dg), decreased the growth rate and optical density of streptococcus bovis jb1 20%, but it had an even greater effect on stationary phase cultures. control cultures receiving only glucose (2 mg/ml) lysed very slowly (<5% decline in optical density in 48 h), but cultures that had been grown with glucose and 2-dg (2 mg/ml each) lysed much faster (>85% decline in optical density in 48 h). cultures that were treated with inhibitors that decrease ...19979462960
ruminal microbial and fermentative changes associated with experimentally induced subacute acidosis in steers.we used six ruminally cannulated steers in a two-period crossover design to study ruminal fermentative and microbial changes associated with induced subacute acidosis. steers were adapted to either an 80% alfalfa hay (hay-adapted)- or corn grain (grain-adapted)-based concentrate diet. after feed was withheld for 24 h, steers were overfed with an all-grain diet at 3.5 x nem daily for 3 d. ruminal contents and jugular blood samples were collected before withholding feed and at 0 and 12 h daily for ...19989464904
a review of bloat in feedlot cattle.improvements in feedlot management practices and the use of various feed additives have reduced, but not eliminated, the occurrence of bloat in feedlot cattle. feedlot bloat reduces the profitability of production by compromising animal performance and more directly by causing fatalities. in feedlots, bloat is associated with the ingestion of large amounts of rapidly fermented cereal grain and destabilization of the microbial populations of the rumen. an abundance of rapidly fermented carbohydra ...19989464911
streptococcus sanguis bacteremia and colorectal cancer.streptococcal bacteremia is an uncommon presentation for colorectal malignancy, yet most physicians are aware of the association between streptococcus bovis infection and these malignancies. many are unaware, however, that other streptococcal species are associated with colon and rectal cancers. in this case report and brief review, we highlight this association and discuss a case of streptococcus sanguis bacteremia associated with an early invasive rectal cancer.19989496877
relationship between intracellular phosphate, proton motive force, and rate of nongrowth energy dissipation (energy spilling) in streptococcus bovis jb1.when the rate of glucose addition to nongrowing streptococcus bovis cell suspensions was increased, the fermentation was homolactic, fructose-1,6-diphosphate (fdp) increased, intracellular inorganic phosphate (p(i)) declined, and the energy-spilling rate increased. atp and adp were not significantly affected by glucose consumption rate, but the decrease in p(i) was sufficient to cause an increase in the free energy of atp hydrolysis (delta g'p). the increase in delta g'p was correlated with an i ...19989501437
in-vitro activity of quinupristin/dalfopristin compared with other widely used antibiotics against strains isolated from patients with endocarditis.the activity of quinupristin/dalfopristin was compared with that of other widely used antibiotics against 355 strains isolated from patients with endocarditis. mics were determined by a standard agar dilution method. quinupristin/dalfopristin was inhibitory at 1 mg/l for all coagulase-negative staphylococci (n = 36) and for the majority of staphylococcus aureus strains (n = 87). the activity of quinupristin/dalfopristin against 186 viridans streptococci was somewhat dependent on the species, wit ...19979511068
[meningitis due to streptococcus bovis in a healthy woman]. 19989522587
bile-esculin test for presumptive identification of enterococci and streptococci: effects of bile concentration, inoculation technique, and incubation time.the bile-esculin test is used to differentiate enterococci and group d streptococci from non-group d viridans group streptococci. the effects on test performance of the concentration of bile salts, inoculum, and duration of incubation were examined with 110 strains of enterococci, 30 strains of streptococcus bovis, and 110 strains of non-group d viridans group streptococci. optimal sensitivity (> 99%) and specificity (97%) of the bile-esculin test can be obtained with a bile concentration of 40% ...19989542954
lack of surface receptors not restriction-modification system determines f4 phage resistance in streptococcus bovis ii/1.the resistance of streptococcus bovis strain ii/1, the producer of sbvi restriction endonuclease, to f4 phage infection was demonstrated by the double-agar-layer method. despite the presence of restriction endonuclease sbvi which can cleave f4 phage dna to numerous fragments in vitro, the evidence that adsorption inhibition is the most important defence mechanism in phage resistance of s. bovis ii/1 strain was obtained by adhesion experiments in vivo. electron microscopy of phage-host mixtures s ...19989569625
inhibition effect of enterocin ccm 4231 in the rumen fluid environment.enterocin ccm 4231 is a bacteriocin with a broad antimicrobial spectrum produced by the ruminal strain enterococcus faecium ccm 4231. its inhibitory effect towards enterococci, ent. faecium ef 26/42, staphylococci, streptococcus bovis ao 24/85 and escherichia coli, as well as towards listeria monocytogenes ohio strain, in the rumen fluid environment was studied during culture at 37 and 30 degrees c for 24 h and 20 days. enterocin ccm 4231 was added to the samples at a concentration of 3200 au ml ...19989569712
effects of chlorhexidine diacetate on ruminal microorganisms.the objectives of this study were to examine the effects of chlorhexidine diacetate on growth and l-lactate production by streptococcus bovis jb1 as well as the effects of this antimicrobial compound on the mixed ruminal microorganism fermentation. addition of 1.8 microm chlorhexidine diacetate to glucose medium resulted in a lag in growth by s. bovis jb1, and growth was completely inhibited in the presence of 3.6, 9.0, and 18 microm chlorhexidine. when 6.2 microm chlorhexidine diacetate was add ...19989608746
a method for the selective enumeration and isolation of ruminal lactobacillus and streptococcus.ruminal lactic acid-producing bacteria were selectively isolated and enumerated using a one hour aerobic exposure prior to incubation on a semi-selective lactobacillus medium, mrs, under anaerobic conditions. the technique allowed growth of pure cultures of ruminal lactobacillus spp. and streptococcus bovis without supporting the growth of pure cultures of any of the prominent ruminal bacterial species. in mixed cultures, the one hour aerobic pre-incubation inhibited the growth of the obligate a ...19989633088
the diversion of lactose carbon through the tagatose pathway reduces the intracellular fructose 1,6-bisphosphate and growth rate of streptococcus bovis.twenty strains of streptococcus bovis grew more slowly on lactose (1.21 +/- 0.12 h-1) then than on glucose (1.67 +/- 0.12 h-1), and repeated transfers or prolonged growth in continuous culture (more than 200 generations each) did not enhance the growth rate on lactose. lactose transport activity was poorly correlated with growth rate, and slow growth could not be explained by the atp production rate (catabolic rate). batch cultures growing on lactose always had less intracellular fructose 1,6-bi ...19989650258
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