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infective endocarditis due to streptococcus gallolyticus associated with colonic displasia.streptococcus gallolyticus is a microorganism belonging to the streptococcus bovis i group isolated in humans, bovines and equines pigeons, among other animals. streptococcus bovis is a streptococcus strain found in the rumen, and has been isolated in the milk of animals with mastitis. the authors describe a case of an adult immunocompetent patient with underlying valvular heart disease, with bacteraemia and infective endocarditis by streptococcus gallolyticus, in whom adenomatous colonic polyps ...201122521029
the association of streptococcus bovis/gallolyticus with colorectal tumors: the nature and the underlying mechanisms of its etiological role.streptococcus bovis (s. bovis) bacteria are associated with colorectal cancer and adenoma. s. bovis is currently named s. gallolyticus. 25 to 80% of patients with s. bovis/gallolyticus bacteremia have concomitant colorectal tumors. colonic neoplasia may arise years after the presentation of bacteremia or infectious endocarditis of s. bovis/gallolyticus. the presence of s. bovis/gallolyticus bacteremia and/or endocarditis is also related to the presence of villous or tubular-villous adenomas in t ...201121247505
[descriptive analysis of diseases associated with streptococcus bovis bacteremia. suggestion of a first protocol of study.]background and objective: it is well-known the relationship between streptococcus bovis (s. bovis) bacteremia and colon cancer, liver cirrhosis and others neoplasms. however, a study protocol to rule out these underlying diseases has not been carried out yet. our objective was to describe s. bovis bacteremia and associated diseases. patients and method: multicenter, retrospective cohort study. s. bovis bacteremias episodes between 2001 and 2009 were included. mean variables: colon neoplasm, non- ...201121719046
two weeks of postsurgical therapy may be enough for high-risk cases of endocarditis caused by streptococcus viridans or streptococcus bovis.clin microbiol infect abstract: the duration of antimicrobial therapy after surgery for infective endocarditis (ie) is controversial. a short course of postsurgical therapy is currently accepted only for patients with negative valve culture. we performed a retrospective (1994-2008) analysis of patients who underwent surgery for ie in our hospital and had a high risk of complications ( one of more of the following: <2-áweeks of antibiotic treatment before surgery; embolism; perivalvular extension ...201121733030
[diagnostic approach to streptococcus bovis bacteremia, or the limits of the evidence-based medicine.] 201121719047
streptococcus bovis peritonitis complicating peritoneal dialysis--a review of 10 years' experience.objective: an association of streptococcus bovis bacteremia with carcinoma of colon has been reported, but data regarding peritoneal dialysis (pd) peritonitis caused by s. bovis is scarce. in this study, we examined the clinical characteristics, associations, and outcomes of this diseaseentity. ? methods: the case records of patients with s. bovis pd peritonitis presenting to 2 renal centers between january 2000 and september 2010 were reviewed. clinical features and outcomes were identified and ...201121804137
re-identification of streptococcus bovis isolates causing bacteraemia according with the new taxonomy criteria: still an issue?all streptococcus bovis blood culture isolates recovered from jan-2003 to jan-2010 (n=52) in our institution were re-identified using new taxonomic criteria based upon their genetic traits. initial identification was performed by the semiautomatic wider system (fco. soria-melguizo, spain) and the api 20 strep (biomérieux, france). all isolates were re-identified by pcr amplification and sequencing of both 16s rdna and soda genes and by mass spectrometry using maldi-tof ms (bruker, germany). resu ...201121752968
[streptococcus bovis endocarditis that presented a difficulty in differentiating from urinary tract infection and collagen disease]. 201122479729
streptococcus bovis isolated in haemoculture a signal of malignant lesion of the colon.s. bovis is known for causing bacteremia and endocarditis as well as accompanying malignant diseases of the gastrointestinal tract. hence, identification of this species and recognition of the clinical characteristics of infections caused are essential for both therapy and prognosis. s. bovis isolated from haemoculture requires an immediate search for gastrointestinal tract lesions.201122239034
how a bowel tumour led to meningitis.this report summarises a case history in which a 75-year-old man was admitted with bacterial meningitis caused by streptococcus bovis. a subsequent abdominal investigation in the light of the known association between invasive s bovis infection and colonic cancer confirmed the suspicion of a cancer located to the rectum. the patient died from clostridium difficile enterocolitis with sepsis and multi-organ failure. this is a highly relevant and interesting case presentation, which besides providi ...201122692778
Selective Antibody Response to Streptococcus gallolyticus Pilus Proteins in Colorectal Cancer Patients.Streptococcus gallolyticus subsp. gallolyticus (previously called Streptococcus bovis biotype I) infections have long been associated with colorectal cancer (CRC). This work aimed to investigate the CRC-associated humoral immune response to four pilus proteins of this bacterium by newly developed ELISAs. Pilus proteins are interesting diagnostic targets as they are the building blocks of pilin-like structures that mediate bacterial virulence and are readily exposed to the host immune system upon ...201122012878
role of lipid ii and membrane thickness in the mechanism of action of the lantibiotic bovicin hc5.lantibiotics are antimicrobial peptides produced by gram-positive bacteria, nisin being the most well-known member. nisin inhibits peptidoglycan synthesis and forms pores at sensitive membranes upon interaction with lipid ii, the essential bacterial cell wall precursor. bovicin hc5, a bacteriocin produced by streptococcus bovis hc5, has the putative n-terminal lipid ii binding motif, and we investigated the mode of action of bovicin hc5 using both living bacteria and model membranes, with specia ...201121876041
streptococcus bovis bacteremia and colorectal carcinoma. 201121921348
significant association of streptococcus bovis with malignant gastrointestinal diseases.streptococcus bovis is a gram-positive bacterium causing serious human infections, including endocarditis and bacteremia, and is usually associated with underlying disease. the aims of the current study were to compare prevalence of the bacterium associated with malignant and nonmalignant gastrointestinal diseases and to determine the susceptibility of the isolated strains to different antimicrobial agents. the result showed that the prevalence of s. bovis in stool specimens from patients with m ...201122121365
efficacy and safety of outpatient parenteral antibiotic therapy for infective endocarditis: a ten-year prospective study.background: the length of treatment of infective endocarditis (ie) with parenteral antibiotics varies from 2 to 6 weeks. although several studies indicate that outpatient parenteral antibiotic treatment (opat) could be safe for uncomplicated viridans-group streptococci (vgs) ie, the experience in spain is limited and data on other types of endocarditis and opat are scarce worldwide. methods: prospective single center study of a cohort including all patients with ie admitted to the hospital clini ...201121723004
long-term gastrointestinal outcomes after streptococcus bovis bacteraemia. 201121995699
streptococcus bovis infections, colorectal cancer and liver dysfunction. 201122023511
differentiation of species of the streptococcus bovis/equinus-complex by maldi-tof mass spectrometry in comparison to soda sequence analyses.the streptococcus bovis/equinus complex is a heterogeneous group within the group d streptococci with important clinical relevance regarding infective endocarditis, sepsis and colon carcinoma. the taxonomic identification of species and sub-species of this complex, by the standard methods remains difficult. in the present study, we compared the cluster analysis of 88 strains of species of the s. bovis/equinus complex by sequence analysis of the manganese-dependent superoxide dismutase gene (soda ...201121247715
[microorganisms and cancer: scientific evidence and new hypotheses].microorganism involvement in cancer has been known for over a century, and different types of parasites, bacteria and viruses have been associated with oncogenic processes. among the bacteria, the first recognised was helicobacter pylori which causes gastric cancer and might be related to extra-gastric cancer in humans. helicobacter hepaticus has been associated with liver cancers using animal models. other bacteria, such as chlamydia psitacii, borrelia burgdorferi and streptococcus bovis, have ...201121292247
monitoring intestinal microbiota profile: a promising method for the ultraearly detection of colorectal cancer.colorectal cancer is one of the most common cancers and is very hard to be detected at an ultraearly stage because of lack of valuable predicating methods that often lead to treatment failure. intestinal microbiota has long been considered to implicate in colorectal cancer pathology; and many recent reports point out a close linkage between the intestinal bacteria and the genesis of the tumor. present studies indicate that the structure and characteristics of the intestinal microbiota are signif ...201121310543
increased dietary zinc oxide changes the bacterial core and enterobacterial composition in the ileum of piglets.this study was conducted to investigate the effects of increased dietary zno on the bacterial core and enterobacterial composition in the small intestine of piglets that were fed diets containing a total of 124 or 3,042 mg of zn per kilogram of diet, respectively. zinc was supplemented to the basal diet as zno. bacterial 16s rrna genes of ileal dna extracts were pcr-amplified with 2 bar-coded primer sets and sequenced by 454 pyrosequencing. the bacterial core species were calculated from the rel ...201121383037
novel clues on the specific association of streptococcus gallolyticus subsp gallolyticus with colorectal cancer.the prevalence of streptococcus gallolyticus subsp gallolyticus ( streptococcus bovis biotype i) endocarditis is in general low but very often linked to colorectal cancer. therefore, this study aimed to reveal the virulence characteristics that distinguish this opportunistic pathogen from a panel of (closely related) intestinal bacteria.201121451000
effects of adding polyclonal antibody preparations on ruminal fermentation patterns and digestibility of cows fed different energy sources.nine ruminally cannulated cows fed different energy sources were used to evaluate an avian-derived polyclonal antibody preparation (pap-mv) against the specific ruminal bacteria streptococcus bovis, fusobacterium necrophorum, clostridium aminophilum, peptostreptococcus anaerobius and clostridium sticklandii and monensin (mon) on ruminal fermentation patterns and in vivo digestibility. the experimental design was three latin squares 3 x 3 distinguished by the main energy source in the diet [dry-g ...201121551346
spontaneous bacterial peritonitis secondary to streptococcus bovis in a patient with chronic chylous ascites.spontaneous bacterial peritonitis (sbp) is a common complication of cirrhosis and ascites, and is predominantly caused by enteric organisms. streptococcus bovis is a rare etiology of sbp that was first reported in 1994. since then, few prior reports of sbp secondary to s. bovis have been observed in patients with underlying cirrhosis or hepatitis. we now present a case of sbp caused by s. bovis in the setting of chronic chylous ascites in a patient with no known liver pathology.201121602688
detection and characterization of pediocin pa-1/ach like bacteriocin producing lactic acid bacteria.fifty-five bacteriocinogenic lactic acid bacteria (lab) isolated from seven different sources. eight isolates were found to produce pediocin pa-1 like bacteriocin as detected by pedb gene pcr and dot-blot hybridization. the culture filtrate (cf) activity of these isolates exhibited strong antilisterial, antibacterial activity against tested food-borne pathogens and lab. the identification and genetic diversity among the selected lab was performed by conventional morphological and molecular tools ...201121656248
antimicrobial activity of daptomycin tested against gram-positive strains collected in european hospitals: results from 7 years of resistance surveillance (2003-2009).daptomycin is a cyclic lipopeptide approved by the european medicines agency (emea) for the treatment of complicated skin and soft tissue infections (cssti) and staphylococcus aureus bacteremia and endocarditis. we evaluated the in vitro activity of daptomycin and comparators tested against clinical isolates from european hospitals over a 7-year period (2003-2009). a total of 36,769 consecutive isolates were collected in 34 medical centers located in 13 european countries, turkey and israel. the ...201121803696
septic arthritis due to streptococcus bovis in a patient with liver cirrhosis due to hepatitis c virus: case report and literature review.monoarthritis remains a diagnostic challenge in rheumatology and orthopedics. the author reports a case of septic arthritis due to streptococcus bovis after several episodes of joint effusion treated with hyaluronic acid (hylan g-f 20) and methylprednisolone acetate in a 69-year-old patient with liver cirrhosis due to hepatitis c virus. neither adenoma of the colon nor endocarditis was present. the diagnostic possibilities for this case of monoarthritis, the pathologies related to the microorgan ...201121953003
dissection of the bridging pattern of bovicin hj50, a lantibiotic containing a characteristic disulfide bridge.bovicin hj50, a lantibiotic produced by streptococcus bovis hj50, is featured by the presence of a disulfide bridge. this study described a simplified in vitro synthetic strategy for producing bovicin hj50 totally based on escherichia coli expression system. in this strategy termed as semi-in vitro biosynthesis (sivb), prepeptide bova and modification enzyme bovm were co-expressed to generate posttranslationally modified bova. then a specific protease bovt150 was employed to remove the leader pe ...201120630724
infected aneurysm of brachial artery after mitral valve infective endocarditis.we present a case of brachial artery infected aneurysm in a patient with infective endocarditis caused by streptococcus bovis. a 49-year-old man presented with fever dyspnea and a pansystolic murmur with irradiation to axilla. the echocardiogram revealed vegetation in native mitral valve. after mitral valve replacement with bioprosthesis, it was observed pulsatile mass of five centimeters in diameter at antecubital fossa of right upper limb. it was made the diagnosis of infected aneurysm of the ...201121881723
involvement of two-component signal transduction system, comde, in the regulation of growth and genetic transformation, in the ruminal bacterium streptococcus bovis.in ruminants, streptococcus bovis is considered to be associated with acute rumen acidosis. to elucidate the regulatory mechanisms of s. bovis growth, we investigated the function of the two components of the peptide pheromone-signaling system, comd and come, which are encoded by comd and come, respectively, via the competence-stimulating peptide comc, which is encoded by comc. deletion of entire comc and two-thirds of comd resulted in decreased growth rate, which may be related to the change in ...201020478389
spontaneous bacterial peritonitis caused by streptococcus bovis: case report and review of the literature.spontaneous bacterial peritonitis (sbp) is a frequent and severe complication that occurs in patient with cirrhosis and ascites. it occurs in 10% to 30% of patients admitted to hospital. the organisms that cause sbp are predominantly enteric. escherichia coli is the most frequent recovered pathogen, and gram-positive bacteria, mainly staphylococcus spp., are being considered an emerging causative agent of sbp. streptococcus bovis that may be found as part of the commensal bowel flora in about 10 ...201020835516
[aortic endocarditis and spondylodiscitis due to streptococcus bovis in a patient in his eighties with colon cancer]. 201020399536
the incidence of bacteremia after argon plasma coagulation in patients with chronic radiation proctocolitis.abstract aim: argon plasma coagulation (apc) is considered a safe treatment for hemorrhagic chronic radiation proctocolitis (crpc), but bacteremia is a rare complication. the study aimed to evaluate its frequency after apc for crpc. method: a prospective study of 21 patients who underwent apc (30 procedures) for crpc was carried out. blood cultures (bactec(r)) were obtained: before and 30 minutes after the procedure (60 samples total). patients were monitored for 48 hours after the procedure to ...201020402735
streptococcus bovis meningitis and hemorrhoids.we report a case of streptococcus bovis (streptococcus gallolyticus subsp. pasteurianus) meningitis, a rare cause of central nervous system (cns) infection in an adult, and comment on the importance of investigation of the lower gastrointestinal tract to identify a portal of entry in cases of systemic streptococcus bovis infection.201020421434
streptococcus bovis bacteraemia revisited: clinical and microbiological correlates in a contemporary series of 59 patients.to characterise the clinical features, associations and outcome in a contemporary series of patients with streptococcus bovis bacteraemia (sbb).201020659498
bacterial meningitis in cirrhotic patients: case series and description of the prognostic role of acute renal failure.to analyze the mortality risk factors in cirrhotic patients with bacterial meningitis (bm).201020453662
increased exposure to bacterial antigen rpl7/l12 in early stage colorectal cancer patients.cancer 2010. (c) 2010 american cancer society. : intestinal bacteria have long been implicated in colorectal cancer pathology, and many reports point to a close linkage between streptococcus bovis biotype i (recently renamed streptococcus gallolyticus) infections and tumors of the human colon. this work aims to investigate the humoral immune response to this bacterium during different stages of colorectal cancer.201020564125
age-dependent profile of left-sided infective endocarditis: a 3-center experience.the influence of age on the main epidemiological, clinical, echocardiographic, microbiological, and prognostic features of patients with infective endocarditis remains unknown. we present the series with the largest numbers and range of ages of subjects to date that analyzes the influence of age on the main characteristics of patients with isolated left-sided infective endocarditis. furthermore, this series is the first one in which patients have been distributed according to age quartile.201020142448
streptococcus gallolyticus subspecies pasteurianus (biotype ii/2), a newly reported cause of adult meningitis.we report the first case of adult meningitis confirmed to be due to streptococcus gallolyticus subsp. pasteurianus. phenotypically reported as streptococcus bovis biotype ii/2, 16s rrna sequencing revealed s. gallolyticus subsp. pasteurianus. because of taxonomic uncertainties, s. gallolyticus subsp. pasteurianus may be an underrecognized agent of systemic infections.201020357211
effects of acarbose on ruminal fermentation, blood metabolites and microbial profile involved in ruminal acidosis in lactating cows fed a high-carbohydrate ration.the objective was to evaluate the effects of an inhibitor of alpha-amylase and glucosidase (acarbose, pfizer limited, corby, uk) on ruminal fermentation, blood metabolism and microbial profile in dairy cows in a 2x2 cross-over experiment. eight holstein cows fitted with rumen cannulas (milk yield, 24.3+/-2.35 kg/d, body weight, 622+/-54 kg, days in milk, 183+/-67, 5 multiparous and 3 primiparous) were used. treatments were: control (no additive, ctr) and alpha-amylase and glucosidase inhibitor ( ...201020053317
ph dynamics and bacterial community composition in the rumen of lactating dairy cows.the influence of ph dynamics on ruminal bacterial community composition was studied in 8 ruminally cannulated holstein cows fitted with indwelling electrodes that recorded ph at 10-min intervals over a 54-h period. cows were fed a silage-based total mixed ration supplemented with monensin. ruminal samples were collected each day just before feeding and at 3 and 6h after feeding. solid and liquid phases were separated at collection, and extracted dna was subjected to pcr amplification followed by ...201020059926
dark hypopyon in streptococcus bovis endogenous endophthalmitis: clinicopathologic correlations.the aim of this report is to present a previously unreported causative organism associated with brown-pigmented hypopyon in a patient with endophthalmitis.201021475652
molecular characterization and significance of phosphoenolpyruvate carboxykinase in a ruminal bacterium, streptococcus bovis.to gain knowledge about the significance of phosphoenolpyruvate (pep) carboxykinase (pck) in streptococcus bovis, the sequence of the gene encoding pck (pck) was determined. transcriptional analysis indicated that the pck is transcribed in a monocistronic fashion. the level of pck-mrna was higher when cells were grown on lactose than on glucose, suggesting that pck synthesis increases when the growth rate is low. the pck-mrna level was higher in a mutant lacking ccpa, which encodes the catabolit ...201020513959
activity of solvent extracts of prosopis spicigera, zingiber officinale and trachyspermum ammi against multidrug resistant bacterial and fungal strains.the emerging trends of multidrug resistance among several groups of microorganisms against different classes of antibiotics led different researchers to develop efficient drugs from plant sources to counter multidrug resistant strains. this study investigated different solvent extracts of prosopis spicigera (p. spicigera), zingiber officinale, and trachyspermum ammi (t. ammi) to determine their efficacy against multidrug resistant microbes.201020539061
acute cholecystitis and bacteraemia due to streptococcus bovis biotype ii. 201021195508
infective endocarditis caused by streptococcus bovis complicated by a superior mesenteric artery mycotic aneurysm and systemic septic emboli in a patient with colon diverticulitis.to date, streptococcus bovis endocarditis complicated by a superior mesenteric artery mycotic aneurysm and systemic septic emboli in a patient with colon diverticulitis has never been reported. a 46-year-old man, with a history of colon diverticulitis identified by diagnostic colon fiberscope and with hypertension, presented with a 14-day history of intermittent fever. an echocardiogram revealed severe mitral regurgitation with two large floating vegetations attached to both mitral leaflets. a b ...201020932486
description of development of rumen ecosystem by pcr assay in milk-fed, weaned and finished lambs in an intensive fattening system.this study examined the reticulo-rumen characteristics of the microbial community and its fermentative characteristics in milk-fed, at weaning and finished lambs in a conventional fattening system. five lambs were assigned to each of three groups: milk-fed lambs slaughtered at 30 days (t30), weaned lambs slaughtered at 45 days (t45) and 'finished lambs' slaughtered at 90 days (t90). at slaughter, rumen size, fermentation parameters (ph, volatile fatty acids and microbial enzyme activity) and pro ...201020050953
identification of tetracycline- and erythromycin-resistant gram-positive cocci within the fermenting microflora of an italian dairy food product.microbiological and molecular analysis of antibiotic resistance in gram-positive cocci derived from the italian pdo (protected designation of origin) dairy food product mozzarella di bufala campana.201020092542
genome sequence of streptococcus gallolyticus: insights into its adaptation to the bovine rumen and its ability to cause endocarditis.streptococcus gallolyticus (formerly known as streptococcus bovis biotype i) is an increasing cause of endocarditis among streptococci and frequently associated with colon cancer. s. gallolyticus is part of the rumen flora but also a cause of disease in ruminants as well as in birds. here we report the complete nucleotide sequence of strain ucn34, responsible for endocarditis in a patient also suffering from colon cancer. analysis of the 2,239 proteins encoded by its 2,350-kb-long genome reveale ...201020139183
special considerations apply when streptococcus bovis is the culprit pathogen. 201020159404
identification of lactic acid bacteria associated with the production of plaa-som, a traditional fermented fish product of thailand.plaa-som is a thai fermented fish product for which whole fish or fish fillets are fermented with either cooked rice or steamed sticky rice, salt, and garlic. a total of 762 lactic acid bacteria (lab) were isolated during plaa-som fermentation by culture on caco(3)-mrs agar plates. they were screened and grouped by amplified ribosomal dna restriction analysis (ardra), giving six groups that were identified by ribosomal dna sequencing as lactococcus garvieae, streptococcus bovis, weissella cibari ...201020167386
streptococcus bovis endocarditis, a silent sign for colonic tumour.an association between colonic carcinoma and streptococcus bovis endocarditis/bacteraemia was first suggested in 1951. this knowledge has great clinical implications, yet we found scant awareness amongst general surgical trainees. the aim of this article was to review the evidence available in the literature and explore the pathophysiology behind this association.201019226366
a case of streptococcus gallolyticus subsp. gallolyticus infective endocarditis with colon cancer: identification by 16s ribosomal dna sequencing.although the association between streptococcus bovis endocarditis and colon carcinoma is well known, very few cases of s. bovis infection associated with underlying malignancies have been reported in korea. the s. bovis group has been recently reclassified and renamed as streptococcus gallolyticus and streptococcus infantarius subspecies under a new nomenclature system. we report a case of infective endocarditis with colon cancer caused by s. gallolyticus subsp. gallolyticus (previously named s. ...201020445334
effects of hops (humulus lupulus l.) extract on volatile fatty acid production by rumen bacteria.to determine the effects of hops extract on in vitro volatile fatty acid (vfa) production by bovine rumen micro-organisms.201020456526
high temporal and inter-individual variation detected in the human ileal microbiota.the diversity and temporal stability of the predominant bacteria in the human ileum was studied with the use of ileal effluent samples of seven individuals with brooke ileostomies. the total number of bacteria within the ileal effluent was in the range of 10⁷ -10⁸ bacteria per gram (wet weight). the diversity of the bacteria in the ileal effluent showed marked differences compared with that in faecal samples from age-matched healthy adults. the ileal effluent had a higher relative abundance of s ...201020626454
rumen microbial population dynamics during adaptation to a high-grain diet.high-grain adaptation programs are widely used with feedlot cattle to balance enhanced growth performance against the risk of acidosis. this adaptation to a high-grain diet from a high-forage diet is known to change the rumen microbial population structure and help establish a stable microbial population within the rumen. therefore, to evaluate bacterial population dynamics during adaptation to a high-grain diet, 4 ruminally cannulated beef steers were adapted to a high-grain diet using a step-u ...201020851965
association of symptoms with gastrointestinal microbiota in irritable bowel syndrome.to investigate the correlations between self-reported symptoms of irritable bowel syndrome (ibs) and the gastrointestinal (gi) microbiota composition.201020857523
[streptococcus bovis endocarditis in the four native valves. a case description]. 201019729229
streptococcus bovis bacteremia related to colon adenoma in a chronic hemodialysis patient.abstract we report the case of a 54-year-old hemodialysis patient who presented with recurrent fever due to streptococcus bovis bacteremia related to colonic tubulovillous adenoma. following polypectomy and broad-spectrum antibiotic therapy, the patient totally recovered. in this paper, we discussed the relation between s. bovis bacteremia, colonic adenomas, and hemodialysis. awareness of this association is critical for early diagnosis and management.201019758303
streptococcus bovis infection of total hip arthroplasty in association with carcinoma of colon.streptococcus bovis is normally found in the gastrointestinal tract of the human population. the association between streptococcus bovis and bowel neoplasia has been frequently reported. this report presents two cases of total hip arthroplasty with streptococcus bovis infection at 18 and 11 years after arthroplasty. the diagnosis was made with the help of clinical findings, standard radiographs, and laboratory tests (complete blood count, c-reactive protein, sedimentation rate, and the hip aspir ...201020727310
novel strategy for yeast construction using delta-integration and cell fusion to efficiently produce ethanol from raw starch.we developed a novel strategy for constructing yeast to improve levels of amylase gene expression and the practical potential of yeast by combining delta-integration and polyploidization through cell fusion. streptococcus bovis alpha-amylase and rhizopus oryzae glucoamylase/alpha-agglutinin fusion protein genes were integrated into haploid yeast strains. diploid strains were constructed from these haploid strains by mating, and then a tetraploid strain was constructed by cell fusion. the alpha-a ...201019707752
effect of lactobacillus casei on streptococcus bovis in faecal flora.bacteraemia caused by streptococcus bovis is often associated with colorectal tumours. also, experimental studies have been proposed that s bovis acts as a promoter of tumours. we report the case of a man with colon adenoma who had a high proportion of s bovis in his faecal flora. he was treated with a lactobacillus casei preparation (blp) and the effect on the faecal flora was examined. l casei reduced the proportion of s bovis (from 43% down to 9%), and the effect continued after the administr ...201022242064
is it antiphospholipid syndrome?the diagnosis of bacterial endocarditis remains a challenge, as nearly half of cases develop in the absence of preexistent heart disease and known risk factors. not infrequently, a blunted clinical course at onset can lead to erroneous diagnoses. we present the case of a 47-year-old previously healthy man in which a presumptive diagnosis of antiphospholipid syndrome was made based on the absence of echocardiographically detected heart involvement, a negative blood culture, normal c-reactive prot ...201021318137
infectious endocarditis due to streptococcus bovis in a patient with colon carcinoma.we report the case of a 66 year-old female patient with infectious endocarditis due to streptococcus bovis and adenocarcinoma of the colon that developed acute aortic insufficiency. she was submitted to aortic valve replacement surgery and later to tumor resection (right hemicolectomy). it is important to emphasize the need for complementing the study of the colon, even in asymptomatic individuals, when infectious endocarditis due to s. bovis is diagnosed.201020944897
microbial and viral pathogens in colorectal cancer.the heterogenetic and sporadic nature of colorectal cancer has led to many epidemiological associations with causes of this disease. as our understanding of the underlying molecular processes in colorectal-cancer develops, the concept of microbial-epithelial interactions as an oncogenic trigger might provide a plausible hypothesis for the pathogenesis of colorectal cancer. by contrast with other cancers of the gastrointestinal tract (gastric carcinoma, mucosa-associated lymphoid-tissue lymphoma) ...201021067973
autoregulation of lantibiotic bovicin hj50 biosynthesis by the bovk-bovr two-component signal transduction system in streptococcus bovis hj50.streptococcus bovis hj50 produces a lacticin 481-like 33-amino-acid-residue lantibiotic, designated bovicin hj50. bovk-bovr in the bovicin hj50 biosynthetic gene cluster is predicted to be a two-component signal transduction system involved in sensing signals and regulating gene expression. disruption of bovk or bovr resulted in the abrogation of bovicin hj50 production, suggesting both genes play important roles in bovicin hj50 biosynthesis. addition of exogenous bovicin hj50 peptide to culture ...201021075878
prevalence of colorectal cancer associated with streptococcus bovis among inflammatory bowel and chronic gastrointestinal tract disease patients.colorectal cancer (crc) is the second most common cause of cancer mortality among men and women worldwide; the risk of its occurrence has been shown to be increased by chronic bacterial infections. a case control study was therefore carried out at hospital universiti sains malaysia (husm) to determine the incidence of colorectal cancer associated with s. bovis infection. a total of 166 stool specimens were collected from diseased patients and healthy individuals and s. bovis isolates were identi ...201021338230
gene cluster for biosynthesis of thermophilin 1277--a lantibiotic produced by streptococcus thermophilus sbt1277, and heterologous expression of tepi, a novel immunity peptide.to identify genes cluster for thermophilin 1277 produced by streptococcus thermophilus sbt1277.201021182576
rumen chemical and bacterial changes during stepwise adaptation to a high-concentrate diet in goats.the correlation between rumen chemical and bacterial changes was investigated during a four periodical stepwise adaptation to a high-concentrate diet (concentrate level at 0%, 30%, 50% and 70% for diet i to iv, respectively) in goats. the results showed that ruminal ph decreased from 6.7 to 5.5 after switching from diet i to ii, and was maintained at about 5.5 on diet iii. denaturing gradient gel electrophoresis results showed that the rumen bacterial community was relatively stable during the i ...201022443874
streptococcus sanguis endocarditis associated with colonic carcinoma.infective endocarditis caused by streptococcus bovis is known to be associated with colorectal malignancy. other less common streptococci, specifically streptococcus sanguis, can be similarly associated with gastrointestinal carcinoma. we present a case of disseminated colorectal carcinoma occurring after a confirmed s sanguis endocarditis, that required mitral valve surgery. there may be a need for gastrointestinal surveillance in patients presenting with bacteraemia caused by less common strep ...201022347887
[recurrent infective endocarditis. a case report].we report the case of a 63-year old patient presenting five infective endocarditis (ie) in a 13-years period, with different types of streptococcus. no entrance way had been found until the last relapse occurring on mitro-aortic bioprosthesis. this recurrence leads to the diagnosis of caecal adenocarcinoma by coloscopy, preceded and guided by pet scanner. it seems to be the initial entrance way which had been unrecognized because of its small size and its particular location, that is very diffic ...201018930174
production of bacteriocins by streptococcus bovis strains from australian ruminants.to examine the prevalence of bacteriocin production in streptococcus bovis isolates from australian ruminants and the feasibility of industrial production of bacteriocin.201019614851
characteristics of xanthan gum-based biodegradable superporous hydrogel.a novel biopolymer-based superporous hydrogel (sph) was synthesized through chemical crosslinking by graft copolymerization of 2-hydroxyethyl methacrylate (hema) and acrylic acid (aa) on to xanthan gum (xg) via redox initiator system of ammonium persulfate (aps) and n, n, n', n'-tetramethylethylenediamine (tmed), in the presence of n, n'-methylenebisacrylamide (mba) crosslinking agent, sodium bicarbonate foaming agent, a triblock copolymer of polyoxyethylene/polyoxypropylene/polyoxyethylene as a ...200919643130
neonatal bacterial meningitis caused by streptococcus gallolyticus subsp. pasteurianus.this report describes a case of neonatal bacteraemia and meningitis due to streptococcus gallolyticus subsp. pasteurianus. based on the identification kit results, this species may have been reported as streptococcus bovis or s. bovis biotype ii. the accurate identification of this organism is mandatory for evaluating the aetiology of neonatal meningitis.200919528187
relative penicillin resistance in streptococcus bovis. a case of neonatal meningitis. 200919712186
characteristics of the bovicin hj50 gene cluster in streptococcus bovis hj50.bovicin hj50 is a new lantibiotic containing a disulfide bridge produced by streptococcus bovis hj50; its encoding gene bova was reported in our previous publication. to identify other genes involved in bovicin hj50 production, dna fragments flanking bova were cloned and sequenced. the bovicin hj50 biosynthesis gene locus was encoded by a 9.9 kb region of chromosomal dna and consisted of at least nine genes in the following order: bova, -m, -t, -e, -f, orf1, orf2, bovk and bovr. a thiol-disulfid ...200919202107
antimicrobial activity of five herbal extracts against multi drug resistant (mdr) strains of bacteria and fungus of clinical origin.antimicrobial activities of the crude ethanolic extracts of five plants were screened against multidrug resistant (mdr) strains of escherichia coli, klebsiella pneumoniae and candida albicans. atcc strains of streptococcus mutans, staphylococcus aureus, enterococcus faecalis, streptococcus bovis, pseudimonas aeruginosa, salmonella typhimurium, escherichia coli, klebsiella pneumoniae and candida albicans were also tested. the strains that showed resistance against the maximum number of antibiotic ...200919214149
pathogen detection in milk samples by ligation detection reaction-mediated universal array method.this paper describes a new dna chip, based on the use of a ligation detection reaction coupled to a universal array, developed to detect and analyze, directly from milk samples, microbial pathogens known to cause bovine, ovine, and caprine mastitis or to be responsible for foodborne intoxication or infection, or both. probes were designed for the identification of 15 different bacterial groups: staphylococcus aureus, streptococcus agalactiae, nonaureus staphylococci, streptococcus bovis, strepto ...200919528580
screening of surface properties and antagonistic substances production by lactic acid bacteria isolated from the mammary gland of healthy and mastitic cows.bovine mastitis (bm) is a costly disease in dairy cattle production. the prevention and treatment of mastitis is performed by applying antimicrobial products that negatively affect milk quality. in the last years, the use of probiotic microorganisms to prevent infections in humans and animals has being aggressively studied. samples from teat canal and milk (foremilk and stripping) were taken from healthy and mastitic mammary quarters. a screening of the surface properties and antagonistic substa ...200919041199
efficient production of optically pure d-lactic acid from raw corn starch by using a genetically modified l-lactate dehydrogenase gene-deficient and alpha-amylase-secreting lactobacillus plantarum strain.in order to achieve direct and efficient fermentation of optically pure d-lactic acid from raw corn starch, we constructed l-lactate dehydrogenase gene (ldhl1)-deficient lactobacillus plantarum and introduced a plasmid encoding streptococcus bovis 148 alpha-amylase (amya). the resulting strain produced only d-lactic acid from glucose and successfully expressed amya. with the aid of secreting amya, direct d-lactic acid fermentation from raw corn starch was accomplished. after 48 h of fermentation ...200919011066
the ability of non-bacteriocin producing streptococcus bovis strains to bind and transfer bovicin hc5 to other sensitive bacteria.streptococcus bovis hc5 produces a broad spectrum lantibiotic (bovicin hc5), but s. bovis jb1 does not have antimicrobial activity. preliminary experiments revealed an anomaly. when s. bovis jb1 cells were washed in stationary phase s. bovis hc5 cell-free culture supernatant, the s. bovis jb1 cells were subsequently able to inhibit hyper-ammonia producing ruminal bacteria (clostridium sticklandii, clostridium aminophilum and peptostreptococcus anaerobius). other non-bacteriocin producing s. bovi ...200919171197
the effect of carbon and nitrogen sources on bovicin hc5 production by streptococcus bovis hc5.to investigate the effect of media composition and agroindustrial residues on bovicin hc5 production by streptococcus bovis hc5.200919320950
biotransformation of 2,4,6-trinitrotoluene by pure culture ruminal bacteria.twenty-one ruminal bacteria species were tested for their ability to degrade 2,4,6-trinitrotoluene (tnt) within 24 h. butyrivibrio fibrisolvens, fibrobacter succinogenes, lactobacillus vitulinus, selenomonas ruminantium, streptococcus caprinus, and succinivibrio dextrinosolvens were able to completely degrade 100 mg/l tnt, with <5% of the original tnt recovered as diaminonitrotoluene metabolites. eubacterium ruminantium, lactobacillus ruminis, ruminobacter amylophilus, streptococcus bovis, and w ...200918839246
properties and role of glyceraldehyde-3-phosphate dehydrogenase in the control of fermentation pattern and growth in a ruminal bacterium, streptococcus bovis.to clarify the control of glycolysis and the fermentation pattern in streptococcus bovis, the molecular and enzymatic properties of nad(+)-specific glyceraldehyde-3-phosphate dehydrogenase (gapdh) were examined. the gapdh gene (gapa) was found to cluster with several others, including those that encode phosphoglycerate kinase and translation elongation factor g, however, gapa was transcribed in a monocistronic fashion. since biochemical properties, such as optimal ph and affinity for glyceraldeh ...200919034572
a pyogenic discitis at c3-c4 with associated ventral epidural abscess involving c1-c4 after intradiscal oxygen-ozone chemonucleolysis: a case report.a case report and clinical discussion.200919365239
[septic shock caused by streptococcus bovis in a haemodialysis patient]. 200919668325
[novel clinical and epidemiologic trends in elderly infective endocarditis].the age of peak incidence of infective endocarditis (ie) has shifted from 45 years in the 50's to 70 years at the present time. elderly persons carry a five times higher risk of ie than the general adult population. factors accounting for this phenomenon include increased use of invasive procedures and implanted medical devices as well as a higher prevalence of degenerative valve disease. in the elderly, ie onset may be insidious, often masked by one or more underlying conditions such as cancer, ...200919708302
ecology and pathogenicity of gastrointestinal streptococcus bovis.streptococcus bovis is an indigenous resident in the gastrointestinal tracts of both humans and animals. s. bovis is one of the major causes of bacterial endocarditis and has been implicated in the incidence of human colon cancer, possibly due to chronic inflammatory response at the site of intestinal colonization. certain feeding regimens in ruminants can lead to overgrowth of s. bovis in the rumen, resulting in the over-production of lactate and capsular polysaccharide causing acute ruminal ac ...200919100852
development of novel cell surface display in corynebacterium glutamicum using porin.we have developed a novel cell surface display in corynebacterium glutamicum using porin proteins as anchor proteins. porins are localized at c. glutamicum mycolic acid layer and exist as a hexamer. we used alpha-amylase from streptococcus bovis 148 (amya) as a model protein to be displayed on the c. glutamicum cell surface. amya was fused to the c terminus of the porins porb, porc, or porh. expression vectors using fused proteins under the control of the cspb promoter were constructed and intro ...200919430772
aorto-pulmonary fistula as a late complication of multiple valve replacement after streptococcus bovis endocarditis in a patient with occult colon carcinoma.we report a case of aorto-pulmonary fistula following multiple valve surgery in a patient with colon carcinoma who had had recurrent episodes of streptococcus bovis endocarditis. diagnosis was made by transesophageal echocardiography and aortography. patient underwent emergency percutaneous implantation of an aortic endoprosthesis, but he continued to deteriorate and the fatal outcome could not be changed. this case underscores the importance of examination of gastrointestinal tract in patients ...200919253356
association of streptococcus pluranimalium with valvular endocarditis and septicaemia in adult broiler parents.the genus streptococcus consists of more than 60 species, but only streptococcus equi subspecies zooepidemicus, streptococcus gallolyticus ssp. gallolyticus, streptococcus gallinaceus, streptococcus dysgalactiae, streptococcus mutans and streptococcus suis have been isolated from poultry. during investigations of the aetiology of increased mortality in broiler parent stock at the end of production, pure cultures of streptococcal-like organisms that could not be classified among these six species ...200919322715
the clinical importance of microbiological findings in the diagnosis and management of bloodstream infections.bloodstream infections are associated with high morbidity and mortality. accurate identification of blood isolates to the species level and identification of the source of infection and/or the portal of entry are crucial for optimal management of these infections. these investigations-in addition to clinical findings and laboratory and imaging studies-are central to informing and directing efficient and effective diagnostic examinations and to choosing the optimal antimicrobial regimen. four cas ...200919374579
physiological changes in rumen fermentation during acidosis induction and its control using a multivalent polyclonal antibody preparation in heifers.physiological changes in rumen fermentation during acidosis induction and its control using a multivalent polyclonal antibody preparation (pap) were studied in a completely randomized experiment using 12 crossbred heifers (452 +/- 20 kg of bw). treatments were control (ctr) or pap. the acidosis induction protocol consisted of 3 periods: 3 mo of 100% fescue hay fed for ad libitum intake, 10 d (from d 1 to 10 of the experiment) of adaptation to the treatment (100% forage feeding + 10 ml/d of pap t ...200919213715
bovicin hc5 inhibits wasteful amino acid degradation by mixed ruminal bacteria in vitro.streptococcus bovis hc5 produces a broad spectrum lantibiotic (bovicin hc5) that inhibits pure cultures of hyper ammonia-producing bacteria (hab). experiments were preformed to see if: (1) s. bovis hc5 cells could inhibit the deamination of amino acids by mixed ruminal bacteria taken directly from a cow, (2) semi-purified bovicin was as effective as s. bovis hc5 cells, and 3) semi-purified and the feed additive monensin were affecting the same types of ammonia-producing ruminal bacteria. because ...200919191869
effects of dietary changes and yeast culture (saccharomyces cerevisiae) on rumen microbial fermentation of holstein heifers.the effects of a dietary challenge to induce digestive upsets and supplementation with yeast culture on rumen microbial fermentation were studied using 12 holstein heifers (277 +/- 28 kg of bw) fitted with a ruminal cannula, in a crossover design with 2 periods of 5 wk. in each period, after 3 wk of adaptation to a 100% forage diet, the dietary challenge consisted of increasing the amount of grain at a rate of 2.5 kg/d (as-fed basis) over a period of 4 d, until a 10:90 forage:concentrate diet wa ...200919542509
association between streptococcus bovis and colon cancer. 200919189926
streptococcus bovis causing perforating corneal ulcer.the purpose of this study was to report the first case of streptococcus bovis causing a perforating corneal ulcer in a healthy adult.200919092424
direct production of cadaverine from soluble starch using corynebacterium glutamicum coexpressing alpha-amylase and lysine decarboxylase.here, we demonstrated the one-step production of cadaverine from starch using a corynebacterium glutamicum strain coexpressing streptococcus bovis 148 alpha-amylase (amya) and escherichia coli k-12 lysine decarboxylase (cada). we constructed the e. coli-c. glutamicum shuttle vector, which produces cada under the control of the high constitutive expression (hce) promoter, and transformed this vector into c. glutamicum css secreting amya. the engineered c. glutamicum expressed both cada and amya, ...200918989633
a collagen-binding adhesin, acb, and ten other putative mscramm and pilus family proteins of streptococcus gallolyticus subsp. gallolyticus (streptococcus bovis group, biotype i).members of the streptococcus bovis group are important causes of endocarditis. however, factors associated with their pathogenicity, such as adhesins, remain uncharacterized. we recently demonstrated that endocarditis-derived streptococcus gallolyticus subsp. gallolyticus isolates frequently adhere to extracellular matrix (ecm) proteins. here, we generated a draft genome sequence of an ecm protein-adherent s. gallolyticus subsp. gallolyticus strain and found, by genome-wide analyses, 11 predicte ...200919717590
streptococcus bovis bacteraemia: identification within organism complex and association with endocarditis and colonic malignancy.to identify streptococcus bovis bacteraemia episodes and their clinical associations, including differences in associations by s. bovis biotypes (or species).200918972318
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