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isolation and identification of adherent epimural bacteria during succession in young lambs.successive changes in aerobic and anaerobic bacterial counts and changes in the generic composition of the epimural community in lambs from 1 to 10 weeks were determined. bacterial culture counts revealed a predominantly anaerobic community, with the mean anaerobic count being 1.4 x 10(7) cfu/cm2 of tissue surface. the aerobic count was highest at 1 week of age and declined significantly thereafter to a mean of 1.8 x 10(4) cfu/cm2, thus representing only 0.13% of the mean anaerobic count after w ...19846721489
compositions and characteristics of strains of streptococcus bovis.streptococcus bovis strains jb1, 26, 581axy2, 21096c, and 45s1 grew on glucose, maltose, starch, sucrose, cellobiose, and lactose. none of these strains grew on xylose or ribose, but arabinose was a suitable energy source for strains 2109c and k27ff4. all strains grew at 45 degrees c, but incubation at 50 degrees c prevented growth. growth was permitted in 2% sodium chloride, but 6.5% sodium chloride was inhibitory. doubling times ranged from 24 to 27 min, and final ph on glucose was approximate ...19846205028
a note on a modified membrane-bovis agar for the enumeration of streptococcus bovis by membrane filtration.a modified membrane-bovis agar, containing a reduced quantity of sodium azide, for the isolation and enumeration of streptococcus bovis is described and evaluated. higher counts, with larger colonies, were obtained from water and sewage samples with this medium than with the original formulation.19846706885
streptococcus bovis endocarditis and its association with colonic carcinoma.two cases of colonic carcinoma detected in patients with streptococcus bovis endocarditis are presented. neither patient had any complaints referable to the gastrointestinal tract; gastrointestinal evaluations were undertaken and colonic lesions were identified purely on the basis of the patients' underlying endocarditis. both patients underwent successful surgery after completion of appropriate antibiotic treatment for s. bovis endocarditis. results of a literature search for similar cases are ...19846468203
the association of streptococcus bovis fecal carriage and colon neoplasia: possible relationship with polyps and their premalignant potential.we designed a study to evaluate the fecal carrier rate of streptococcus bovis in patients with endoscopically proven colonic polyps. benign polyps (n = 63), i.e., hyperplastic, inflammatory, and juvenile, had a similar fecal carrier rate as the normal control colons. colons with polyps that are at increased risk for malignant degeneration (n = 62), i.e., tubulovillous and villous adenomas, and colons with carcinoma (n = 18), had a statistically significant increase (p less than 0.05) in the feca ...19853966453
a case of streptococcus bovis endocarditis and colonic carcinoma. 19853972551
changing pattern of infective endocarditis.there has been a significant trend toward an increase in the age of patients with endocarditis, and it seems likely that the age of these patients will continue to increase as the population ages. the proportion of patients with endocarditis who have rheumatic heart disease as an underlying lesion has decreased from about 40 percent in patients studied from 1950 to 1970 to about 25 percent in more recent series, and this trend will probably continue. prosthetic valves and degenerative heart dise ...19854014278
seasonal changes in the ruminal microflora of the high-arctic svalbard reindeer (rangifer tarandus platyrhynchus).the dominant rumen bacteria in high-arctic svalbard reindeer were characterized, their population densities were estimated, and ruminal ph was determined in summer, when food quality and availability are good, and in winter, when they are poor. in summer the total cultured viable population density was (2.09 +/- 1.26) x 10(10) cells ml-1, whereas in winter it was (0.36 +/- 0.29) x 10(10) cells ml-1, representing a decrease to 17% of the summer population density. on culture, butyrivibrio fibriso ...19854026289
growth inhibition of streptococcus mutans by cellular extracts of human intestinal lactic acid bacteria.the in vitro growth of streptococcus mutans was completely inhibited by water-soluble extracts from cells of various intestinal lactic acid bacteria identified as streptococcus faecium, streptococcus equinus, lactobacillus fermentum, and lactobacillus salivarius. the growth inhibition was dependent on the concentrations of the extracts. in contrast, the extracts did not inhibit the growth of the major indigenous intestinal lactic acid bacteria isolated from humans. these lactic acid bacteria wer ...19854030098
brain-abscess caused by streptococcus bovis. 19854092706
streptococcus bovis endocarditis presenting with gastrointestinal bleeding. 19853878313
cross-reactions between alpha-streptococci and omniserum, a polyvalent pneumococcal serum, demonstrated by direct immunofluorescence, immunoelectroosmophoresis, and latex agglutination.in recent years several groups have used serological methods to demonstrate pneumococcal capsular antigens in sputum. in the present study 123 strains of alpha-hemolytic streptococci (including 97 strains from sputum or pharyngeal specimens) were tested for cross-reactions with a polyvalent antipneumococcal serum (omniserum). representatives of the following species were included: streptococcus bovis, s. equinus, s. intermedius, s. lactis, s. milleri, s. mitis, s. mutans, s. sobrinus, s. salivar ...19853889046
in vitro growth of mastitis-associated streptococci in bovine mammary secretions.cell-free, fat-free mammary secretions were tested in vitro for ability to support growth of streptococci associated with mastitis. secretions were obtained prior to drying off, during the dry period, at calving, and during lactation from four cow treatment groups. treatment groups were dry cow therapy, dry cow therapy and mammary glands subjected to induced inflammation 7 d post-drying-off, no dry cow therapy and no induced inflammation, no dry cow therapy but mammary glands subjected to induce ...19853905891
streptococcus bovis endocarditis and bowel neoplasm. 19853862872
streptococcus bovis bacteraemia requires rigorous exclusion of colonic neoplasia and endocarditis.twelve patients presented to the hospitals of the auckland hospital board with bacteraemia caused by streptococcus bovis in the years 1979-84. ten had endocarditis, affecting homograft valves in two cases and the tricuspid valve in one case. of nine patients who underwent investigation of the large bowel, only one did not have a colorectal tumour. three had colonic adenocarcinoma and three had colorectal villous adenoma. two, including a patient with acute hepatic failure from alcoholic cirrhosi ...19854048386
the rumen microbiology of seaweed digestion in orkney sheep.the microbial populations of the rumens of seaweed-fed and pasture-fed orkney sheep were examined. the populations in the pasture-fed sheep were similar to those of other domestic ruminants fed on land plants, but those of the seaweed-fed animals showed major differences in the dominant species. total ciliate populations were quantitatively similar, but in the seaweed-fed animals dasytricha ruminantium was one of the most dominant species. no phycomycete fungi or cellulolytic bacteria were found ...19854030526
antibiotic activity of an isocyanide metabolite of trichoderma hamatum against rumen bacteria.a metabolite of trichoderma hamatum, 3-(3-isocyanocyclopent-2-enylidene)propionic acid, was tested for its effects on growth of and carbohydrate metabolism in 11 strains of functionally important rumen bacteria. to standardize the biological activity of this unstable metabolite, a rapid, aerobic disc diffusion assay was developed using escherichia coli atcc 11775. in an anaerobic broth dilution assay using a medium lacking rumen fluid and containing a soluble carbohydrate, the minimum inhibitory ...19854084861
bacteriological methods for distinguishing between human and animal faecal pollution of water: results of fieldwork in nigeria and zimbabwe.bacteriological techniques have traditionally been used to detect faecal pollution of drinking water supplies. recently, methods have been developed to distinguish between human and animal faecal pollution in temperate climates. the present study assessed the applicability and practicality of these methods in tropical countries. fieldwork in nigeria and zimbabwe has shown that animal faecal pollution can reliably be identified by the detection and enumeration of rhodococcus coprophilus using mod ...19853910299
comparison of single doses of amoxicillin or of amoxicillin-gentamicin for the prevention of endocarditis caused by streptococcus faecalis and by viridans streptococci.recent recommendations for the prophylaxis of endocarditis in humans have advocated single doses or short courses of antibiotic combinations (beta-lactam plus aminoglycoside) for susceptible patients in whom enterococcal bacteremia might develop or for patients at especially high risk of developing endocarditis (e.g., patients with prosthetic cardiac valves). we tested the prophylactic efficacy (in rats with catheter-induced aortic vegetations) of single doses of amoxicillin plus gentamicin agai ...19853925031
regulation of lactate production in streptococcus bovis: a spiraling effect that contributes to rumen acidosis.when streptococcus bovis was grown in batch culture with 6 g/l glucose at ph 6.7, maximum specific growth rate was 1.47 h(-1), and lactate was the primary fermentation product. in continuous culture at ph 6.7 and growth rate equal to .10 h(-1), little lactate was formed, and formate, acetate, and ethanol accounted for most of the product. when extra-cellular ph decreased to 4.7, intra-cellular ph declined to 5.4, and organisms switched back to lactate production. intracellular concentration of f ...198521800456
vertebral osteomyelitis caused by streptococcus bovis.the association of streptococcus bovis septicemia with abnominal neoplasms has been well-documented. the case of a man who presented with vertebral osteomyelitis due to s. bovis is reported; he was subsequently found to have bacteremia and colonic polyps. it is recommended that patients who present with localized as well as generalized s. bovis infections undergo yearly colonoscopy.19863946470
streptococcal endocarditis: single vs. combination antibiotic therapy and role of various species.forty-eight patients with 51 episodes of infective endocarditis caused by streptococci were studied in a comparison of the efficacy of single antibiotic therapy versus that of combination therapy. there was no statistical difference in terms of clinical course, toxicity, and mortality between the group given a single drug and that given combination therapy. however, one patient experienced two relapses, in both instances after high-dose penicillin therapy. the species and antibiotic susceptibili ...19863952425
[endocarditis caused by streptococcus bovis and subjacent gastrointestinal disease]. 19863563296
the effect of ph on the growth and metabolism of streptococcus bovis in continuous culture.streptococcus bovis h13/1 was grown anaerobically at phs between 5.0 and 6.5 in a glucose-limited chemostat at a dilution rate of 0.05/h. the growth yield and the production of acetate, ethanol and formate decreased at phs less than 6.5 whereas the production of lactate increased at the lower ph values. when a culture was subjected to sequential ph changes, growth yield and fermentation products were influenced not only by the ph existing in the culture medium but also by the metabolic activity ...19863781936
streptococcus bovis bacteremia and underlying gastrointestinal neoplasms.two patients are described in whom streptococcus bovis bacteremia was the only clue to the presence of a colonic neoplasm. a third patient had the rare association of gastric carcinoma and streptococcus bovis bacteremia that followed an operation for the carcinoma. the need for both complete gastrointestinal survey in patients with streptococcus bovis bacteremia and administration of appropriate antibiotic prophylaxis during surgical manipulation is emphasized.19863784983
phosphoenolpyruvate-dependent phosphorylation of hexoses by ruminal bacteria: evidence for the phosphotransferase transport system.six species of ruminal bacteria were surveyed for the phosphoenolpyruvate (pep)-dependent phosphorylation of glucose. selenomonas ruminantium hd4, streptococcus bovis jb1, and megasphaera elsdenii b159 all showed significant activity, but butyrivibrio fibrisolvens 49, bacteroides succinogenes s85, and bacteroides ruminicola b1(4) showed low rates of pep-dependent phosphorylation and much higher rates in the presence of atp. s. ruminantium hd4, s. bovis jb1, and m. elsdenii b159 also used pep to ...19863789722
[right-left shunt in tricuspid endocarditis caused by streptococcus bovis]. 19862950416
streptococcus bovis endocarditis with vertebral osteomyelitis. 19863750018
evaluation of aztreonam in the treatment of spontaneous bacterial peritonitis in patients with cirrhosis.to determine the efficacy of aztreonam in the treatment of spontaneous bacterial peritonitis in patients with hepatic cirrhosis, 14 patients (7 males, 7 females) with 16 gram-negative infective episodes (12 escherichia coli and 4 klebsiella pneumoniae) were treated with aztreonam infusions at doses of 1 gm per 8 hr for a planned 14-day period. ages ranged from 40 to 75 years with a mean of 57 +/- 10 years. all organisms were highly susceptible to aztreonam (minimal inhibitory concentration less ...19863530945
growth of gram-positive mastogenic bacteria in normal, simulated bulk tank, and mastitic milk held at simulated fluctuating temperatures of farm bulk tank.growth of staphylococcus aureus, staphylococcus epidermidis, streptococcus agalactiae, streptococcus dysgalactiae, streptococcus bovis, and streptococcus uberis was studied in normal milk, simulated bulk tank milk, and aseptic mastitic milk held at simulated fluctuating temperatures of farm bulk tank for 48 h. with the exception of s. bovis, growth rates of the other five bacteria were similar in both normal and simulated bulk tank milk. mastitic milk inhibited growth of all bacteria studied. a ...19863531259
infective endocarditis due to penicillin-tolerant streptococcus bovis.two streptococcus bovis isolates obtained from patients with endocarditis were found to be tolerant to penicillin and other cell wall active agents. by time-kill analysis, penicillin and streptomycin acted synergistically against these strains. the existence of tolerant s. bovis strains should be considered when initially choosing antibiotics for the treatment of serious s. bovis infections.19863720266
antibodies to bacterial vaccines demonstrating specificity for human choriogonadotropin (hcg) and immunochemical detection of hcg-like factor in subcellular bacterial fractions.investigations were done to determine whether vaccines prepared with chemically killed staphylococcus haemolyticus ru1 and streptococcus bovis av46 (bacteria that have been demonstrated to express human choriogonadotropin [hcg]-like material on their surface) elicited antibodies in rabbits with specificity for hcg determinants. in addition, the anatomical locus of the hcg-like factor was determined by separation of bacterial subcellular fractions. the results demonstrated that these bacterial va ...19863721581
stoichiometry of glucose and starch splitting by strains of amylolytic bacteria from the rumen and anaerobic digester.the stoichiometry of glucose and starch splitting by the amylolytic bacteria streptococcus bovis, selenomonas ruminantium, butyrivibrio fibrisolvens, eubacterium ruminantium and clostridium sp. was followed. there were many differences in the ratios of metabolites and in growth yields, as well as in the cell composition, between the growth on glucose and starch. the bacteria employ different nutritional strategies with respect to both energy sources.19863759723
presumptive identification of "streptococcus milleri" in 5 h.rapid miniaturized tests for acetoin production, arginine hydrolysis, and sorbitol fermentation were used for presumptive identification of non-beta-hemolytic "streptococcus milleri" isolates in 5 h. all 77 "s. milleri" strains tested were voges-proskauer positive, arginine hydrolysis positive, and sorbitol fermentation negative. on the basis of these reactions, "s. milleri" was differentiated from isolates of other viridans group streptococcal species and from streptococcus bovis.19863760146
streptococcus bovis catheter infection and the short bowel syndrome.streptococcus bovis bacteremia has been associated with several gastrointestinal disorders, most notably carcinoma of the colon. this report describes a 57-year-old woman with short bowel syndrome in whom s. bovis bacteremia and an infection of an indwelling parenteral nutrition catheter developed. a barium enema revealed diverticula and a foreshortened small intestine. this case implicates the short bowel syndrome in the pathogenesis of s. bovis bacteremia and supports empiric antibiotic covera ...19863963050
[tricuspid endocarditis with right-left auricular shunt through a patent foramen ovale].the authors report the case of a tricuspid endocarditis secondary to streptococcus bovis with important regurgitation and severe hypoxemia secondary to a right-left atrial shunt through a patent foramen ovale, requiring a surgical treatment which included the replacement of the tricuspid valve and closure of the dehiscence in the inter-atrial septum. the presence of a patent foramen ovale in the course of a tricuspid endocarditis has been exceptionally reported. this diagnosis deserves to be evo ...19873548566
factors affecting the rate of breakdown of bacterial protein in rumen fluid.1. the cellular proteins of butyrivibrio fibrisolvens, lactobacillus casei, megasphaera elsdenii, selenomonas ruminantium and streptococcus bovis were labelled by growth in the presence of l-[14c]leucine, and the breakdown of labelled protein was measured in incubations of these bacteria with rumen fluid to which unlabelled 5 mm-l-leucine was added. the rate of protein breakdown was estimated from the rate of release of radioactivity into acid-soluble material. 2. protein breakdown occurred at d ...19873118940
role of immunoblotting in the diagnosis of culture negative and enterococcal endocarditis.serum samples from patients with endocarditis and septicaemia due to enterococcus faecalis, enterococcus faecium, streptococcus bovis, and streptococcus sanguis were immunoblotted against antigenic extracts from all four species. in e faecalis endocarditis there was a strong igm response to e faecalis antigenic bands of 112, 88-90, and 45-47 kd and a strong igg response to 88-90 and 45-47 kd bands. in e faecium endocarditis there was a pronounced igg response to an e faecium band of 82-90 kd. fo ...19873119674
[use of the colonizing preparation amylastin in the stimulation of rumen-type digestion in young ruminants].in experiments with calves during the milk-nutrition period we tested the use of the colonisation preparation amylastim, containing a bacterial strain of streptococcus bovis ao 24/85, as a factor speeding up the development of the rumen microflora. we gave the preparation to the animals for a period of four weeks and studied its influence on the microflora adherent to the epithelium of the rumen wall. we ascertained a significant increase in the number of streptococcus bovis germs (p less than 0 ...19873127979
effects of potassium ion concentrations on the antimicrobial activities of ionophores against ruminal anaerobes.the antimicrobial activities of monensin and lasalocid against representative strains of ruminal bacteria were evaluated in medium containing three different concentrations of potassium (1.3, 7.9, or 23.3 mm). the growth of eubacterium ruminantium was inhibited by low concentrations of ionophores (less than or equal to 0.16 mg/liter), while the strain of streptococcus bovis tested was resistant to high concentrations of ionophores (40 mg/liter) at all potassium concentrations tested. the mics of ...19873426214
interactions between rumen amylolytic and lactate-utilizing bacteria in growth on starch.the growth and metabolism of the rumen amylolytic bacteria streptococcus bovis, butyrivibrio fibrisolvens and bacteroides ruminicola, growing in pure cultures and co-cultures with the rumen lactilytic bacteria megasphaera elsdenii and veillonella alcalescens were followed. the interaction of amylolytic bacteria with v. alcalescens represents a simple food chain. the interaction with m. elsdenii is more complex, since there is a simultaneous competition for products of the starch degradation.19873429358
noma in an unusual case of perforated appendicitis with pneumoperitoneum.noma is a very rare gangrenous infection of the oral cavity usually associated with debilitating diseases, immunosuppression, or malnutrition. its development in a previously healthy 13-year-old boy with perforated appendicitis is presented. streptococcus bovis type ii and e coli were isolated from the noma and streptococcus bovis, bacteroides fragilis, and bacteroides asacharolyticus from the peritoneal fluid. a transient impaired immune cellular function was found and may have contributed to t ...19873430301
streptococcus bovis bacteraemia associated with candidal lesions of the large bowel in a leukaemic child. a case report.a 5-year-old boy with acute myeloblastic leukaemia had large-bowel masses, demonstrated at autopsy, 5 weeks after an episode of streptococcus bovis bacteraemia. the association of strept. bovis bacteraemia with large-bowel disease, well documented in adults, should be considered in children.19873474796
streptococcus bovis endocarditis and carcinoma of the colon. 19873504316
[aneurysm of the ulnar artery in streptococcus bovis septicemia. first case]. 19872951723
in vitro activity of ly146032 (daptomycin) against selected aerobic bacteria.the in vitro activity of the new lipopeptide antibiotic ly146032 was generally four-fold greater (mic 90 less than or equal to 0.5 microgram/ml) than that of vancomycin against methicillin-susceptible or methicillin-resistant staphylococcus aureus and coagulase-negative species of staphylococcus. enterococci, streptococcus bovis, group b and viridans streptococci, and corynebacterium group j-k isolates were inhibited by less than or equal to 4 micrograms/ml of ly146032, which represented activit ...19873032612
antimicrobial therapy of streptococcal endocarditis.the majority of patients with endocarditis caused by viridans streptococci or streptococcus bovis susceptible to less than or equal to 0.1 mg/l of penicillin may be treated successfully for two weeks with penicillin 20 x 10(6) million units intravenously together with streptomycin 7.5 mg/kg body weight intramuscularly twice daily or gentamicin 1 mg/kg body weight intravenously every 8 h. patients with nutritionally variant viridans streptococcal endocarditis should be treated for four weeks with ...19873316158
susceptibility and resistance of ruminal bacteria to antimicrobial feed additives.susceptibility and resistance of ruminal bacterial species to avoparcin, narasin, salinomycin, thiopeptin, tylosin, virginiamycin, and two new ionophore antibiotics, ro22-6924/004 and ro21-6447/009, were determined. generally, antimicrobial compounds were inhibitory to gram-positive bacteria and those bacteria that have gram-positive-like cell wall structure. mics ranged from 0.09 to 24.0 micrograms/ml. gram-negative bacteria were resistant at the highest concentration tested (48.0 micrograms/ml ...19873116929
therapy of streptococcal endocarditis: correlation of animal model and clinical studies.streptococci (viridans group, streptococcus bovis, enterococci still account for the majority of cases of infective endocarditis in the non-addict population. experimental animal models of endocarditis have been used (primarily in rabbits) to delineate the major therapeutic principles of this disease; viz. bactericidal antimicrobial agents must be given parenterally in high dosages for prolonged period of time. overall, there is a good correlation between results obtained by: (1) in-vitro suscep ...19873316165
streptococcus bovis infection of the pulmonary valve in an elderly woman with a colonic polyp. 19873805562
seasonal changes in the cecal microflora of the high-arctic svalbard reindeer (rangifer tarandus platyrhynchus).the dominant cecal bacteria in the high-arctic svalbard reindeer were characterized, their population densities were estimated, and cecal ph was determined in summer, when food quality and availability is good, and in winter, when it is very poor. in summer the total culturable viable bacterial population was (8.9 +/- 5.3) x 10(8) cells ml-1, whereas in winter it was (1.5 +/- 0.7) x 10(8) cells ml-1, representing a decrease to 17% of the summer population density. of the dominant species of cult ...19873030193
streptococcus bovis meningitis. 19873625223
infective endocarditis: clinical features in young and elderly patients.the elderly constitute an increasing percentage of patients with infective endocarditis. the disease manifestations and outcomes in 53 episodes of endocarditis in patients over the age of 60 were reviewed and compared with 55 episodes of endocarditis in patients less than 40 years of age and 46 episodes of endocarditis in patients aged 40 to 60. the percentage of cases caused by staphylococci and streptococci were roughly equal in all groups. enterococci, streptococcus bovis, and coagulase-negat ...19873674051
ruminal microbial development in conventionally or early-weaned calves.eight bull calves (four holsteins and four ayrshire calves with mean birth weight of 38.8 kg) were ruminally cannulated at 3 d of age and allocated to one of two weaning programs. all calves were fed colostrum for 3 d after birth and milk until weaning. calves in the conventional-weaning program were fed a starter diet from 3 d of age and weaned at 6 wk of age. in the early-weaning program, calves were fed a highly palatable pre-starter diet from 3 d of age until they consumed 227 g/d, and then ...19873571026
lack of association of streptococcus bovis with noncolonic gastrointestinal carcinoma.since previous studies have shown an increased fecal carriage rate of streptococcus bovis in patients with carcinoma of the colon, a study was undertaken to determine the fecal carriage rate of this organism in patients with gastrointestinal carcinoma of noncolonic origin. organisms identified as s. bovis were isolated on fecal culture from 10 of 82 (12%) controls, 19 of 94 (20%) individuals with noncolonic gastrointestinal carcinoma, and 12 of 80 (15%) patients with other gastrointestinal disor ...19873578233
a proposed mechanism of monensin action in inhibiting ruminal bacterial growth: effects on ion flux and protonmotive force.streptococcus bovis, a gram-positive ruminal bacterium, was unable to grow in the presence of monensin. when monensin (5 mg/liter) was added to actively growing cultures, there was an immediate decrease in growth rate, and within 3 h no further growth was observed. glucose utilization and lactate production continued for another 8 h even though growth had ceased. monensin caused a decrease (p less than .05) in intracellular k+, a decrease (p less than .05) in intracellular ph and an increase (p ...19873583956
streptococcus bovis bacteraemia and its association with alimentary-tract neoplasm. 19872880000
incidence of colonic lesions in streptococcus bovis and enterococcal endocarditis. 19872882164
comparative survival of antibiotic-resistant and -sensitive fecal indicator bacteria in estuarine water.the survival of antibiotic-resistant and -sensitive strains of escherichia coli, enterococcus faecalis, enterococcus faecium, streptococcus equinus, and two environmental isolates, ap17 and aq62, was examined in estuarine water. each strain was rendered resistant to a combination of two antibiotics by serial passage in increasing concentrations of antibiotics. cultures were incubated in filter-sterilized estuarine water for up to 7 days. recovery was assessed by examining colony-forming ability ...19873111372
human choriogonadotropin-like material in bacteria of different species: electron microscopy and immunocytochemical studies with monoclonal and polyclonal antibodies.immunocytochemical studies using antisera to whole human choriogonadotropin (hcg), to its alpha- and beta-subunits and to the cooh-terminal peptide of hcg beta, and two monoclonal antibodies to hcg beta, demonstrated expression of hcg-like material, its individual subunits and/or fragments in nine bacterial strains. seven of these were isolated from patients with cancer and were definitely identified as streptococcus faecalis (three strains), staphylococcus haemolyticus (two strains) and staphyl ...19873116165
[infectious endocarditis caused by streptococcus bovis and alcoholic cirrhosis complicated by hepatoma].the case is reported of a 66-year old man who developed streptococcus bovis endocarditis on a fairly loose aortic stenosis and who also presented with alcoholic cirrhosis complicated by an ultimately lethal hepatoma. on this occasion, comments are made on the following points: -str. bovis is increasingly responsible for bacterial endocarditis. this micro-organism is now rapidly and reliably identified. -str. bovis endocarditis has some clinical features of its own. -patients in whom the usual po ...19872823737
transport and phosphorylation of disaccharides by the ruminal bacterium streptococcus bovis.toluene-treated cells of streptococcus bovis jb1 phosphorylated cellobiose, glucose, maltose, and sucrose by the phosphoenolpyruvate-dependent phosphotransferase system. glucose phosphorylation was constitutive, while all three disaccharide systems were inducible. competition experiments indicated that separate phosphotransferase systems (enzymes ii) existed for glucose, maltose, and sucrose. [14c]maltose transport was inhibited by excess (10 mm) glucose and to a lesser extent by sucrose (90 and ...19872827569
[endocarditis or bacteremia caused by streptococcus bovis and colorectal neoplasms].streptococcus bovis is a relatively frequent causative agent of endocarditis or bacteriaemia, particularly in the elderly. in the past s. bovis has often been incompletely or even falsely classified. for therapeutic and prognostic reasons it is important to classify this agent exactly with biochemical methods even in the routine laboratory. endocarditis or bacteriaemia due to s. bovis are often seen in conjunction with malignant, potentially malignant or benign colorectal neoplasias. after endoc ...19883045953
amylolytic activity of selected species of ruminal bacteria.a variety of species of ruminal bacteria were screened for the ability to grow in starch-containing medium and produce amylase. of those tested, the highest levels of amylase were produced by streptococcus bovis jb1 and ruminobacter amylophilus h18. other strains that grew well on starch and produced amylase included butyrivibrio fibrisolvens a38 and 49 and bacteroides ruminicola 23 and b14. varying the carbohydrate source provided for growth resulted in changes in the growth rate and level of a ...19882454075
preservation of capsular material of streptococcal cells by specific lectins determined by immunoelectron microscopy.we describe the use of lectins as specific stabilizing agents for the polysaccharide capsular components of two gram-positive bacteria, streptococcus agalactiae and streptococcus bovis. treatment of bacterial suspensions with wheatgerm agglutinin and concanavalin a allowed better morphological preservation as well as immunoelectron microscopic localization of a capsular component (lipoteichoic acid) by employing specific antibodies and the protein a-gold technique. data obtained indicate that le ...19883071525
the bacteria responsible for ureolysis in artificial dental plaque.the origin of ureolytic activity in artificial-mouth plaques was established by assessing the contribution to plaque ureolytic activity of the isolated bacteria. to overcome losses of ureolytic activity caused by the unstable presence of urease in oral bacteria, ureolytic bacteria were isolated from an exceptionally active plaque (1 mumol nh3/min per mg protein) in which 63 per cent of the flora was ureolytic. after their ability to metabolize urea was stabilized, 13 ureolytic bacteria remained: ...19883075450
effects of the ionophores monensin and tetronasin on simulated development of ruminal lactic acidosis in vitro.a continuous coculture of four ruminal bacteria, megasphaera elsdenii, selenomonas ruminantium, streptococcus bovis, and lactobacillus sp. strain lb17, was used to study the effects of the ionophores monensin and tetronasin on the changes in ruminal microbial ecology that occur during the onset of lactic acidosis. in control incubations, the system simulated the development of lactic acidosis in vivo, with an initial overgrowth of s. bovis when an excess of glucose was added to the fermentor. la ...19883223764
endocarditis due to streptococcus bovis--a case report. 19883249974
fermentation of peptides and amino acids by a monensin-sensitive ruminal peptostreptococcus.a monensin-sensitive ruminal peptostreptococcus was able to grow rapidly (growth rate of 0.5/h) on an enzymatic hydrolysate of casein, but less than 23% of the amino acid nitrogen was ever utilized. when an acid hydrolysate was substituted for the enzymatic digest, more than 31% of the nitrogen was converted to ammonia and cell protein. coculture experiments and synergisms with peptide-degrading strains of bacteroides ruminicola and streptococcus bovis indicated that the peptostreptococcus was u ...19882975156
partial characterisation of the inhibitory substances produced by streptococcus oralis and related species.the production of inhibitory substances was sought by deferred antagonism. streptococcus oralis, streptococcus mitis and streptococcus sanguis produced bactericidal concentrations of hydrogen peroxide, but no detectable bacteriocin. actinomycetes and neisseria pharyngis were particularly susceptible to the lethal action of hydrogen peroxide. streptococcus milleri produced bactericidal concentrations of hydrogen peroxide and bacteriocin-like substances. s. oralis strains were inhibited by the bac ...19883216798
esterase activities in butyrivibrio fibrisolvens strains.thirty strains of butyrivibrio fibrisolvens isolated in diverse geographical locations were examined for esterase activity by using naphthyl esters of acetate, butyrate, caprylate, laurate, and palmitate. all strains possessed some esterase activity, and high levels of activity were observed with strains 49, h17c, s2, actf2, and lm8/1b. esterase activity also was detected in other ruminal bacteria (bacteroides ruminicola, selenomonas ruminantium, ruminobacter amylophilus, and streptococcus bovis ...19883178205
susceptibilities of streptococcal strains associated with infective endocarditis to nine antibiotics.the susceptibilities of 121 streptococcal strains isolated from patients with infective endocarditis to mezlocillin, piperacillin, ceftizoxime, ceftriaxone, ceftazidime, imipenem, ciprofloxacin, ofloxacin and pefloxacin were determined by the agar dilution technique. viridans streptococci, streptococcus bovis and enterococcus faecalis were susceptible to imipenem, mezlocillin and piperacillin. all the strains, except e. faecalis were sensitive to ceftriaxone, ceftizoxime and ceftazidime and resi ...19883391051
two-stage reimplantation in infected total knee arthroplasty.twenty-one infected total knee arthroplasties (tka) in 21 patients were treated from september 1980 through october 1987. of these, 15 were followed for more than one year. treatment of these patients consisted of thorough debridement of all infected tissue and components; a cement spacer was used in ten patients. the cement was impregnated with antibiotics. this procedure was followed for an average of 4.2 weeks with intravenous antibiotics and tka utilizing antibiotic-impregnated cement. five ...19883180576
streptococcus bovis bacteremia and digestive system neoplasms. 19883194717
sodium-dependent transport of neutral amino acids by whole cells and membrane vesicles of streptococcus bovis, a ruminal bacterium.streptococcus bovis jb1 cells were able to transport serine, threonine, or alanine, but only when they were incubated in sodium buffers. if glucose-energized cells were washed in potassium phosphate and suspended in potassium phosphate buffer, there was no detectable uptake. cells deenergized with 2-deoxyglucose and incubated in sodium phosphate buffer were still able to transport serine, and this result indicated that the chemical sodium gradient was capable of driving transport. however, when ...19883136141
streptococcus bovis endocarditis associated with villous adenoma following colonoscopy. 19883177185
colonic villous adenoma, polyp, and leiomyoma presenting with streptococcus bovis endocarditis. 19883336976
differentiation of ruminal bacterial species by enzyme-linked immunosorbent assay using egg yolk antibodies from immunized chicken hens.cross-reactivity among four species of ruminal bacteria was examined by using egg yolk antibodies from immunized leghorn laying hens and an enzyme-linked-immunosorbent assay. the effects of the four species on the hens were compared on various days postimmunization. hens injected with the same bacterial species had similar apparent antibody levels over the entire postimmunization period, but only bacteroides ruminicola b1(4) and selenomonas ruminantium d antigens elicited early increases in appa ...19883355145
[streptococcus bovis bacteremia and colonic diseases].four febrile patients were found to have a bacteraemia or endocarditis caused by streptococcus bovis. although there were no gastrointestinal symptoms, polyps in the colon, of a high degree of malignancy, were discovered in three. in the fourth, carcinoma of the colon, requiring resection, was found seven years after streptococcus bovis endocarditis. the polyps were removed by endoscopy and the streptococcus bovis infection in all cases cured by penicillin. all four patients had accompanying res ...19883371220
lasalocid-catalyzed proton conductance in streptococcus bovis as affected by extracellular potassium.the effect of extracellular potassium on lasalocid-catalyzed proton conductance in streptococcus bovis 24 was measured by using the fluorescent probe 9-aminoacridine. increasing external potassium concentration resulted in decreased proton flux into s. bovis cells exposed to the ionophore. these results suggest that lasalocid catalyzes k+/h+ exchange diffusion in s. bovis cells.19892705771
non-proton-motive-force-dependent sodium efflux from the ruminal bacterium streptococcus bovis: bound versus free pools.growing cells of streptococcus bovis jb1 had a sodium content of 1,125 nmol/mg of protein and, based on a ratio of cell volume to protein of 4.3 microliters/mg, the apparent intracellular sodium concentration was more than 240 mm. much of this sodium could not be removed by water washing even if cells were boiled or treated with the pore-forming ionophore, gramicidin, but it could be exchanged for potassium. stationary cultures had a 2.6-microliters volume per milligram of protein and a total so ...19892481426
studies on faecal streptococci in the river tigris.six hundred water samples collected from the river tigris at mosul city were investigated for faecal streptococci. human faecal streptococci were predominant, and animal faecal streptococci were also detected. eight species and varieties were identified, viz streptococcus faecalis, atypical streptococcus faecalis, streptococcus bovis, streptococcus equinus, streptococcus faecalis var. liquefaciens, streptococcus faecalis var. zymogenes, streptococcus durans and streptococcus faecium. the inciden ...19892739584
isolation and characterization of two rumen streptococcus bovis bacteriophages.a method for the isolation of streptococcus bovis bacteriophages from ruminal fluid of calves is described. thirty to 2 x 10(3) phages per ml infecting streptococcus bovis strains 4/1 and 47/3 were isolated directly from ruminal fluid. two bacteriophages were characterized on the basis of plaque morphology, host ranges, electron microscopic morphology and dna restriction endonuclease digestion patterns. the f1 and f3 phages formed clear plaques of different sizes. the plaque size of the f1 phage ...19892586334
transport of glutamine by streptococcus bovis and conversion of glutamine to pyroglutamic acid and ammonia.streptococcus bovis jb1 cells energized with glucose transported glutamine at a rate of 7 nmol/mg of protein per min at a ph of 5.0 to 7.5; sodium had little effect on the transport rate. because valinomycin-treated cells loaded with k and diluted into na (ph 6.5) to create an artificial delta psi took up little glutamine, it appeared that transport was driven by phosphate-bond energy rather than proton motive force. the kinetics of glutamine transport by glucose-energized cells were biphasic, a ...19892722740
[streptococcus bovis septicemia and colonic cancer].the authors report the case of a patient who developed a streptococcus bovis septicemia in the post-operative course of a surgical cure of an incarcerated umbilical hernia. there were no other complaints. a barium enema showed a malignant tumor of the descending colon. there were hepatic metastases. no complementary treatment was undertaken. the patient died shortly after. in the literature colonic proliferative lesions in association with this germ is reported in a total of 36% of proliferative ...19892497613
diagnosing endocarditis with the cloned 112 kda antigen of enterococcus faecalis.a new indirect elisa is presented for the diagnosis of enterococcal endocarditis. it is based on the cloning of enterococcus faecalis dna into lambda gt11. the library was screened by antisera from three cases of e. faecalis endocarditis. three positive clones were found, all of which cross-reacted with the 112 kda antigen of e. faecalis. one of these was taken and lysogenised into e. coli y1089 to produce a fusion protein of 125 kda. this iptg dependent protein was purified by affinity chromato ...19892509565
nisin inhibits several gram-positive, mastitis-causing pathogens.organisms known to cause bovine mastitis, enterococcus faecalis ssp. liquefaciens atcc 27959, staphylococcus aureus atcc 29740, streptococcus agalactiae atcc 27956, streptococcus equinus atcc 27960, streptococcus dysgalactiae atcc 27957, streptococcus uberis atcc 27958, and the neotype staphylococcus epidermidis atcc 14990 were examined for their susceptibility to the small peptide antibiotic, nisin. using a disc assay, minimum inhibitory concentrations of nisin ranged from 10 to 250 micrograms/ ...19892516858
evaluation of teicoplanin for treatment of endocarditis caused by gram-positive cocci in 20 patients.teicoplanin, a new glycopeptide antibiotic similar to vancomycin, was evaluated for the treatment of bacterial endocarditis in an open multicenter study from may 1985 to august 1987. a total of 20 patients with positive blood culture endocarditis received teicoplanin once daily as a mean intravenous injection of 7.3 mg/kg of body weight (range, 4.8 to 10.6 mg/kg); in 17 patients, teicoplanin was combined with another antibiotic, usually an aminoglycoside. the mean duration of therapy was 28 days ...19892527483
the faecal flora of the giant panda (ailuropoda melanoleuca).the faecal floras of two adult (male and female) and one infant (male) giant panda kept at the ueno zoo, tokyo, japan were examined and shown to be quite different from those of other animals. the predominant bacteria in the adults were streptococcus (including enterococcus) and enterobacteriaceae, while obligate anaerobes had minor populations. fastidious anaerobes were not detected. the predominant bacteria in the suckling infant were lactobacillus and streptococcus, followed by bifidobacteriu ...19892584169
more monensin-sensitive, ammonia-producing bacteria from the rumen.two monensin-sensitive bacteria which utilized carbohydrates poorly and grew rapidly on amino acids were isolated from the bovine rumen. the short rods (strain sr) fermented arginine, serine, lysine, glutamine, and threonine rapidly (greater than 158 nmol/mg of protein per h) and grew faster on casein digest containing short peptides than on free amino acids ().34 versus 0.29 h(-1)). gelatin hydrolysate, an amino acid source containing an abundance of long peptides, was unable to support growth ...19892757371
[infectious endocarditis due to streptococcus bovis with suspected relationship with colon cancer]. 19892794665
dna probe for identification of streptococcus pneumoniae.a total of 287 clinical isolates of streptococcus pneumoniae (pneumococcus) were tested for their ability to undergo autolysis when treated with sodium deoxycholate. the test was positive for all but one isolate, strain doc-1. this autolysis required the activity of an enzyme which is unique and characteristic of s. pneumoniae: a choline-dependent n-acetylmuramoyl-l-alanine amidase, the gene product of the lyta gene. we used lyta as a dna probe to test the distribution of the autolysin gene amon ...19892563386
[a case report of streptococcus bovis meningitis associated with asymptomatic rectal carcinoma]. 19892543721
carcinoma of the colon presenting as streptococcus equinus bacteremia. 19892910086
bacteremia with streptococcus bovis and streptococcus salivarius: clinical correlates of more accurate identification of isolates.two biotypes of streptococcus bovis can be identified by laboratory testing and can be distinguished from the phenotypically similar organism streptococcus salivarius. we assessed the clinical relevance of careful identification of these organisms in 68 patients with streptococcal bacteremia caused by these similar species. s. bovis was more likely to be clinically significant when isolated from blood (89%) than was s. salivarius (23%). there was a striking association between s. bovis i bactere ...19892915024
streptococcus bovis endocarditis and carcinoma of the colon. 19892924063
electrophoretic analysis of ureases in streptococcus salivarius and in saliva.experimental conditions were established for the extraction, electrophoresis and detection of urease isoenzymes from streptococcus salivarius. thiol concentrations were critical and ureases from different strains varied in ease of dissociation. a characteristic pattern was obtained for 30 ureolytic s. salivarius strains isolated from; saliva (6), dental plaque (12), artificial dental plaque (6) and non-oral sources (6), and also for a ureolytic streptococcus bovis from artificial plaque. one non ...19892640315
streptococcus bovis septicemia and large bowel neoplasia.streptococcus bovis septicemia is a relatively uncommon entity that is associated with an increased incidence of colonic neoplasms. three of four patients with s. bovis endocarditis subsequent to septicemia underwent colonoscopy. the fourth patient underwent a barium enema and a proctoscopic examination. polyps were found in three patients, and adenocarcinoma of the colon in one. patients with s. bovis endocarditis should be considered at high risk for colonic neoplasms. screening colonoscopy is ...19892650506
evaluation of the rapid strep system for identification of gram-positive, catalase-negative cocci isolated from bovine intramammary infections.the rapid strep system (analytab products, plainview, ny) was used to identify 199 gram-positive, catalase-negative cocci isolated from bovine intramammary infections. the system accurately identified 88.4% of isolates. the system identified 100% of 46 streptococcus agalactiae, 100% of 48 streptococcus dysgalactiae, 54.5% of 11 streptococcus equinus, and 96.2% of 53 streptococcus uberis isolates. enterococcus spp. were identified correctly 83.3% of the time. one of 4 streptococcus saccharolyticu ...19892689485
streptococcus bovis bacteremia and endocarditis at groote schuur hospital. 19892727863
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