evaluation of two fluorogenic assays for identification of streptococcus species isolated from bovine mammary glands.liquid and agar assays that utilized 4-methylumbelliferyl-conjugated beta-d-glucuronide, beta-d-galactoside, or n-acetyl-beta-d-glucosaminide, and agglutination by dolichos biflorus lectin were evaluated for identification of streptococcus species isolated from bovine mammary glands. a greater number of streptococcus uberis isolates were negative for n-acetyl-beta-d-glucosaminidase by the liquid assay compared with the agar assay. enzyme profiles for streptococcus dysgalactiae were similar by bo ...19911904457
streptococcus bovis septicaemia associated with uncomplicated colonic carcinoma. 19911908347
[group d streptococci and enterococci: identification, sensitivity to antibiotics and a study of the high level resistance to aminosides (charles nicolle hospital in tunis)].197 strains of enterococcus and group d streptococcus were isolated from human material at charles nicolle hospital (tunis) and identified by biochemical tests: 174, enterococcus faecalis, 6 enterococcus faecium, 2 enterococcus durans and 15 streptococcus bovis. the sensitivity to antibiotics was studied: all enterococcus faecium were resistant at least to one antibiotic, 15% of enterococcus faecalis were sensitive for all antibiotics tested the other species were frequently sensitive. high leve ...19911910140
differentiation of enterococci from other group d streptococci by means of a specific monoclonal antibody.we report on the specificity of a monoclonal antibody which reacts with autoclaved extracts of four species of enterococci but does not react to the same extent with similar extracts from two non-enterococcal group d streptococci. the monoclonal antibody also reacts specifically with purified lipoteichoic acid from streptococcus faecalis but not significantly with purified lipoteichoic acid from the non-enterococcal species streptococcus bovis and streptococcus equinus. the specific antigen dete ...19911936940
enumeration by a miniaturized method of escherichia coli, streptococcus bovis and enterococci as indicators of the origin of faecal pollution of waters.counts of escherichia coli, faecal streptococci and enterococci were made on faecal specimens from human and animal origin and urban raw sewage waters, with microtiter plates containing selective substances. escherichia coli was more numerous than faecal streptococci and enterococci in 80% of the samples regardless of the origin. consequently the use of the ratio e. coli/faecal streptococci to distinguish human from animal origin of faecal pollution is questionable. enterococcus faecalis was pre ...19911938678
[cloning the alpha-amylase gene of streptococcus bovis and its expression in bacillus subtilis cells].the gene coding for alpha-amylase from the ruminant bacterium streptococcus bovis was cloned on the plasmid pmx39 in bacillus subtilis cells. an alpha-amylase positive colony was isolated in the initial screening of 3900 colonies on the medium containing insoluble starch. the size of the insert was approximately 2.8 kb. the recombinant plasmid was stably maintained in bacillus subtilis cells under the nonselective conditions.19911944323
a re-assessment of bacterial growth efficiency: the heat production and membrane potential of streptococcus bovis in batch and continuous culture.glucose-limited, continuous cultures (dilution rate 0.1 h-1) of streptococcus bovis jb1 fermented glucose at a rate of 3.9 mumol mg protein-1 h-1 and produced acetate, formate and ethanol. based on a maximum atp yield of 32 cells/mol atp (stouthamer 1973) and 3 atp/glucose, the theoretical glucose consumption for growth would have been 2.1 mumol mg protein-1 h-1. because the maintenance energy requirement was 1.7 mumol/mg protein/h (russell and baldwin 1979), virtually all of the glucose consump ...19911953297
factors influencing the adherence of strains of streptococcus bovis and escherichia coli isolated from ruminal epithelium.two strains of streptococcus bovis (a1 and a5) and one strain of escherichia coli (0141:h28) isolated from the surface of bovine ruminal mucous epithelium were examined for adherence to isolated and cultured ruminal epithelial cells. the e. coli adhered to the target cell by means of fimbriae, which had several common properties with type 1 common fimbriae and caused mannose-sensitive haemagglutination. the a1 strain of s. bovis was devoid of fimbriae and its adherence to the epithelial surface ...19901974372
resistance of streptococcus bovis to acetic acid at low ph: relationship between intracellular ph and anion accumulation.streptococcus bovis jb1, an acid-tolerant ruminal bacterium, was able to grow at phs from 6.7 to 4.5, and 100 mm acetate had little effect on growth rate or proton motive force across the cell membrane. when s. bovis was grown in glucose-limited chemostats at ph 5.2, the addition of sodium acetate (as much as 100 mm) had little effect on the production of bacterial protein. at higher concentrations of sodium acetate (100 to 360 mm), production of bacterial protein declined, but this decrease cou ...19912036013
[prospective study of routine screening for cirrhosis and colonic tumors associated with streptococcus bovis bacteremia].between 1987 and 1990, seven patients hospitalized in our hospital had bacteremia caused by streptococcus bovis. complete gastrointestinal evaluation was routinely carried out for digestive portal of entry and liver disease screening. in four cases (2 bacteremia, 2 endocarditis), a colonic growth was detected: sigmoid adenoma (n = 1) and rectosigmoid carcinoma (n = 4); in one case (endocarditis), several rectosigmoid carcinomas were associated with alcoholic cirrhosis; in one case (bacteremia), ...19912060742
[infected false aneurysm at the level of an aortotomy cicatrix 5 years after mechanical replacement of the aortic valve].the authors report the case of streptococcus bovis infection of a pseudoaneurysm which developed in the aortotomy suture line five years after aortic valve replacement with a bjork-shiley prosthesis. this was an isolated lesion without involvement of the prosthesis or aortic ring and was an operative finding.19902106296
[fever, somnolence, vomiting, diarrhea].a 69-year-old female was admitted because of a febrile state, somnolence, nausea and diarrhea. addison's disease known to have developed several years earlier after adrenal tuberculosis suggested addisonian crisis triggered by an acute infection. therapy with steroids and substitution of electrolytes and fluid led to rapid improvement. streptococcus bovis was identified as causative agent. the known association of this germ with cancer of the colon led to further investigation which revealed can ...19902124719
[community- and hospital-acquired bacteremia: a retrospective study in a regional hospital. iii. microbiological aspects].a retrospective study was made of all blood cultures performed over a 40-month period at the ivrea-castellamonte hospital (turin, italy). a total of 4386 vials from 619 patients were examined. there were 619 positive vials (14.1%) from 131 patients (21.2%) corresponding to 145 bacteremia episodes, including 129 monomicrobial (89%) and 16 polymicrobial (11%). ten patients (1.6%) had more than one episode. there were 73 polluted vials (1.7%). a total of 165 microorganism were isolated: gram-positi ...19902133330
atpase-dependent energy spilling by the ruminal bacterium, streptococcus bovis.when the ruminal bacterium streptococcus bovis was grown in batch culture with glucose as the energy source, the doubling time was approximately 21 min and the rate of bacterial heat production was proportional to the optical density (1.72 microw/micrograms protein). if exponentially growing cultures were treated with chloramphenicol, there was a decline in heat production, but the rate was greater than 0.30 microw/micrograms protein even after growth ceased. since there was no heat production a ...19902140038
formation of a clear zone on tannin-treated brain heart infusion agar by a streptococcus sp. isolated from feces of koalas.gram-positive cocci, isolated from the feces of koalas and identified as streptococcus bovis biotype i, formed a distinct clear zone on tannin-treated brain heart infusion agar, suggesting that this isolate has the unique characteristic of degrading the tannin-protein complex.19902180375
in vitro activity and beta-lactamase stability of the new oral cephalosporin bay v 3522.the activity of the new oral cephalosporin bay v 3522 was compared to that of six other beta-lactam agents. bay v 3522 inhibited methicillin-susceptible staphylococcus aureus and staphylococcus epidermidis at less than or equal to 2 micrograms/ml, compared to mics of greater than or equal to 8 micrograms/ml for the other cephalosporins tested. it was more active against streptococcus pyogenes (mic less than or equal to 0.06 microgram/ml) than cefuroxime, cefixime, cephalexin and cefaclor. groups ...19902226499
streptococcus bovis meningitis: report of 2 cases.we describe 2 cases of streptococcus bovis meningitis and review the 9 cases previously reported. this microorganism is a rare cause of meningitis in which there are no distinctive clinical or laboratory features. the gram stain of the csf is usually negative. ten of the 11 cases had some underlying disease or comorbid condition that predisposed to s bovis infection: gastrointestinal disorder, endocarditis, csf leak, polymyalgia rheumatica, and mandibular block. treatment with high-dose penicill ...19902234437
low-affinity, high-capacity system of glucose transport in the ruminal bacterium streptococcus bovis: evidence for a mechanism of facilitated diffusion.the glucose phosphotransferase system (pts) of streptococcus bovis could not account for the glucose consumption of exponential cultures, and the kinetics of glucose transport were biphasic. a pts-deficient mutant lost the high-affinity, low-capacity system but retained its ability to take up glucose at high substrate concentrations. the low-affinity, high-capacity system did not require a proton motive force or atp and could not be driven by an artificial membrane potential in the presence or a ...19902268149
[endocarditis caused by streptococcus bovis and colorectal neoplasms].to evaluate the association of streptococcus bovis endocarditis with large bowel neoplasms.19902275628
[incidence and severity of cardiac involvement in streptococcus bovis septicemia. report of 10 cases].endocarditis due to the streptococcus bovis is an affection which is more and more often recognized and whose link with the colic tumor pathology has been well established those last few years and confirmed in this study with a frequency of 60 p. cent. however, few studies have stated the gravity of the heart affection in streptococcus bovis septicaemias. in this series of 10 streptococcus bovis septicaemias, the valvular affection is frequent and serious. the vegetations are found in 9 cases ou ...19902291615
vancomycin-aminoglycoside combinations in therapy of endocarditis caused by enterococcus species and streptococcus bovis.between 1974 and 1986, five patients with enterococcal endocarditis (three of whom had a prosthetic valve) and three patients with streptococcus bovis endocarditis were treated with a combination of vancomycin and an aminoglycoside for a mean duration of 40 days. this regimen was prescribed because of allergy to beta-lactam (n = 7) and/or failure of previous treatment (n = 4). three of the eight patients underwent valve replacement. in the six evaluable patients cure was achieved with the vancom ...19902318216
in vivo dental plaque-forming ability and cariogenicity of the bacterium streptococcus bovis in gnotobiotic rats.germ-free fisher rats, fed on a high sucrose diet, were monoinfected with streptococcus bovis strains. high levels of fissure caries were formed by three strains; one strain produced moderate levels; another produced only very low levels. no strain could produce approximal or buccal/lingual lesions. all strains could colonize the fissures of teeth in the lower jaw, as shown by scanning electron microscopy. the most cariogenic strains were associated with caries scores similar to those of strep. ...19902344292
the survival of streptococci and enterococci in animal feed and on straw with particular reference to streptococcus uberis.the survival of streptococcus uberis, strep. bovis, enterococcus faecalis spp. faecalis and ent. faecium in animal feed and on wheat straw was studied for one month. streptococcus bovis survived the best in feed and strep. uberis the worst while on straw ent. faecium was the most resilient species and there was little to choose between the other three. the recovery of all species, excepting strep. bovis, on selective and non-selective agar was comparable. the growth of strep. bovis, on the other ...19902351619
the triad of streptococcus bovis bacteremia, colonic pathology, and liver disease.the association of streptococcus bovis endocarditis and colon carcinoma has been reported previously in small series in the medical, but not surgical, literature. although the fecal carriage rate of s. bovis increases with colonic pathology, no explanation exists for the development of bacteremia in these cases. to explore the possible contribution of hepatic dysfunction to the development of portal and systemic bacteremia, the incidence of both colonic pathology and liver disease or dysfunction ...19902357141
streptococcus bovis bacteraemia: its significance for the colorectal surgeon.patients with streptococcus bovis bacteraemia are at high risk of harbouring an asymptomatic colorectal cancer or adenomatous polyps. it is therefore important to differentiate to a species level any group d streptococci grown in blood cultures. colonoscopy is the preferred method of screening these patients and high dose penicillin is the antibiotic of choice.19902357182
[gastrointestinal disorders and streptococcus bovis bacteremia]. 19902374620
effect of ionophores and ph on growth of streptococcus bovis in batch and continuous culture.batch cultures (ph 6.7) of streptococcus bovis jb1 were severely inhibited by 1.25 and 5 microm lasalocid and monensin, respectively, even though large amounts of glucose remained in the medium. however, continuous cultures tolerated as much as 10 and 20 microm, respectively, and used virtually all of the glucose. although continuous cultures grew with high concentrations of ionophore, the yield of bacterial protein decreased approximately 10-fold. when ph was decreased from 6.7 to 5.7, the pote ...19902383003
streptococcus bovis brain abscess associated with an occult colonic villous adenoma.streptococcus bovis is now recognized as a common cause of bacterial endocarditis. however, infection of the central nervous system by this organism is very rare. recent studies have demonstrated the association between streptococcus bovis infections and colonic neoplasms.19902404406
amylolytic activity of selected species of ruminal bacteria.a variety of species of ruminal bacteria were screened for the ability to grow in starch-containing medium and produce amylase. of those tested, the highest levels of amylase were produced by streptococcus bovis jb1 and ruminobacter amylophilus h18. other strains that grew well on starch and produced amylase included butyrivibrio fibrisolvens a38 and 49 and bacteroides ruminicola 23 and b14. varying the carbohydrate source provided for growth resulted in changes in the growth rate and level of a ...19882454075
non-proton-motive-force-dependent sodium efflux from the ruminal bacterium streptococcus bovis: bound versus free pools.growing cells of streptococcus bovis jb1 had a sodium content of 1,125 nmol/mg of protein and, based on a ratio of cell volume to protein of 4.3 microliters/mg, the apparent intracellular sodium concentration was more than 240 mm. much of this sodium could not be removed by water washing even if cells were boiled or treated with the pore-forming ionophore, gramicidin, but it could be exchanged for potassium. stationary cultures had a 2.6-microliters volume per milligram of protein and a total so ...19892481426
[streptococcus bovis septicemia and colonic cancer].the authors report the case of a patient who developed a streptococcus bovis septicemia in the post-operative course of a surgical cure of an incarcerated umbilical hernia. there were no other complaints. a barium enema showed a malignant tumor of the descending colon. there were hepatic metastases. no complementary treatment was undertaken. the patient died shortly after. in the literature colonic proliferative lesions in association with this germ is reported in a total of 36% of proliferative ...19892497613
diagnosing endocarditis with the cloned 112 kda antigen of enterococcus faecalis.a new indirect elisa is presented for the diagnosis of enterococcal endocarditis. it is based on the cloning of enterococcus faecalis dna into lambda gt11. the library was screened by antisera from three cases of e. faecalis endocarditis. three positive clones were found, all of which cross-reacted with the 112 kda antigen of e. faecalis. one of these was taken and lysogenised into e. coli y1089 to produce a fusion protein of 125 kda. this iptg dependent protein was purified by affinity chromato ...19892509565
nisin inhibits several gram-positive, mastitis-causing pathogens.organisms known to cause bovine mastitis, enterococcus faecalis ssp. liquefaciens atcc 27959, staphylococcus aureus atcc 29740, streptococcus agalactiae atcc 27956, streptococcus equinus atcc 27960, streptococcus dysgalactiae atcc 27957, streptococcus uberis atcc 27958, and the neotype staphylococcus epidermidis atcc 14990 were examined for their susceptibility to the small peptide antibiotic, nisin. using a disc assay, minimum inhibitory concentrations of nisin ranged from 10 to 250 micrograms/ ...19892516858
evaluation of teicoplanin for treatment of endocarditis caused by gram-positive cocci in 20 patients.teicoplanin, a new glycopeptide antibiotic similar to vancomycin, was evaluated for the treatment of bacterial endocarditis in an open multicenter study from may 1985 to august 1987. a total of 20 patients with positive blood culture endocarditis received teicoplanin once daily as a mean intravenous injection of 7.3 mg/kg of body weight (range, 4.8 to 10.6 mg/kg); in 17 patients, teicoplanin was combined with another antibiotic, usually an aminoglycoside. the mean duration of therapy was 28 days ...19892527483
[a case report of streptococcus bovis meningitis associated with asymptomatic rectal carcinoma]. 19892543721
dna probe for identification of streptococcus pneumoniae.a total of 287 clinical isolates of streptococcus pneumoniae (pneumococcus) were tested for their ability to undergo autolysis when treated with sodium deoxycholate. the test was positive for all but one isolate, strain doc-1. this autolysis required the activity of an enzyme which is unique and characteristic of s. pneumoniae: a choline-dependent n-acetylmuramoyl-l-alanine amidase, the gene product of the lyta gene. we used lyta as a dna probe to test the distribution of the autolysin gene amon ...19892563386
rapid identification of streptococcus bovis by using combination constitutive enzyme substrate hydrolyses.several studies have documented the association of blood and rectal-culture positivity for streptococcus bovis with gastrointestinal neoplasia, especially colonic carcinoma. conventional methods using bile-esculin hydrolysis, salt tolerance, and sugar fermentations to differentiate s. bovis from other streptococci are laborious, slow, and relatively expensive. commercially available systems are costly and require at least 24 to 48 h of incubation. a rapid identification procedure for s. bovis an ...19892570081
the faecal flora of the giant panda (ailuropoda melanoleuca).the faecal floras of two adult (male and female) and one infant (male) giant panda kept at the ueno zoo, tokyo, japan were examined and shown to be quite different from those of other animals. the predominant bacteria in the adults were streptococcus (including enterococcus) and enterobacteriaceae, while obligate anaerobes had minor populations. fastidious anaerobes were not detected. the predominant bacteria in the suckling infant were lactobacillus and streptococcus, followed by bifidobacteriu ...19892584169
isolation and characterization of two rumen streptococcus bovis bacteriophages.a method for the isolation of streptococcus bovis bacteriophages from ruminal fluid of calves is described. thirty to 2 x 10(3) phages per ml infecting streptococcus bovis strains 4/1 and 47/3 were isolated directly from ruminal fluid. two bacteriophages were characterized on the basis of plaque morphology, host ranges, electron microscopic morphology and dna restriction endonuclease digestion patterns. the f1 and f3 phages formed clear plaques of different sizes. the plaque size of the f1 phage ...19892586334
electrophoretic analysis of ureases in streptococcus salivarius and in saliva.experimental conditions were established for the extraction, electrophoresis and detection of urease isoenzymes from streptococcus salivarius. thiol concentrations were critical and ureases from different strains varied in ease of dissociation. a characteristic pattern was obtained for 30 ureolytic s. salivarius strains isolated from; saliva (6), dental plaque (12), artificial dental plaque (6) and non-oral sources (6), and also for a ureolytic streptococcus bovis from artificial plaque. one non ...19892640315
streptococcus bovis septicemia and large bowel neoplasia.streptococcus bovis septicemia is a relatively uncommon entity that is associated with an increased incidence of colonic neoplasms. three of four patients with s. bovis endocarditis subsequent to septicemia underwent colonoscopy. the fourth patient underwent a barium enema and a proctoscopic examination. polyps were found in three patients, and adenocarcinoma of the colon in one. patients with s. bovis endocarditis should be considered at high risk for colonic neoplasms. screening colonoscopy is ...19892650506
evaluation of the rapid strep system for identification of gram-positive, catalase-negative cocci isolated from bovine intramammary infections.the rapid strep system (analytab products, plainview, ny) was used to identify 199 gram-positive, catalase-negative cocci isolated from bovine intramammary infections. the system accurately identified 88.4% of isolates. the system identified 100% of 46 streptococcus agalactiae, 100% of 48 streptococcus dysgalactiae, 54.5% of 11 streptococcus equinus, and 96.2% of 53 streptococcus uberis isolates. enterococcus spp. were identified correctly 83.3% of the time. one of 4 streptococcus saccharolyticu ...19892689485
lasalocid-catalyzed proton conductance in streptococcus bovis as affected by extracellular potassium.the effect of extracellular potassium on lasalocid-catalyzed proton conductance in streptococcus bovis 24 was measured by using the fluorescent probe 9-aminoacridine. increasing external potassium concentration resulted in decreased proton flux into s. bovis cells exposed to the ionophore. these results suggest that lasalocid catalyzes k+/h+ exchange diffusion in s. bovis cells.19892705771
transport of glutamine by streptococcus bovis and conversion of glutamine to pyroglutamic acid and ammonia.streptococcus bovis jb1 cells energized with glucose transported glutamine at a rate of 7 nmol/mg of protein per min at a ph of 5.0 to 7.5; sodium had little effect on the transport rate. because valinomycin-treated cells loaded with k and diluted into na (ph 6.5) to create an artificial delta psi took up little glutamine, it appeared that transport was driven by phosphate-bond energy rather than proton motive force. the kinetics of glutamine transport by glucose-energized cells were biphasic, a ...19892722740
streptococcus bovis bacteremia and endocarditis at groote schuur hospital. 19892727863
aerococcus-like organism--an unnoticed urinary tract pathogen.aerococci are only occasionally isolated from urinary tract specimens. a 4-month' survey revealed 29 patients suspected of urinary tract infection (uti), from whom an aerococcus-like organism (alo) was isolated in significant numbers from urinary tract specimens and singly in 11 cases. approximately 0.8% of all urinary tract specimens examined during the period yielded growth of alo. the median age of patients was 75 years. half of the patients were found to have conditions predisposing to uti. ...19892736106
studies on faecal streptococci in the river tigris.six hundred water samples collected from the river tigris at mosul city were investigated for faecal streptococci. human faecal streptococci were predominant, and animal faecal streptococci were also detected. eight species and varieties were identified, viz streptococcus faecalis, atypical streptococcus faecalis, streptococcus bovis, streptococcus equinus, streptococcus faecalis var. liquefaciens, streptococcus faecalis var. zymogenes, streptococcus durans and streptococcus faecium. the inciden ...19892739584
more monensin-sensitive, ammonia-producing bacteria from the rumen.two monensin-sensitive bacteria which utilized carbohydrates poorly and grew rapidly on amino acids were isolated from the bovine rumen. the short rods (strain sr) fermented arginine, serine, lysine, glutamine, and threonine rapidly (greater than 158 nmol/mg of protein per h) and grew faster on casein digest containing short peptides than on free amino acids ().34 versus 0.29 h(-1)). gelatin hydrolysate, an amino acid source containing an abundance of long peptides, was unable to support growth ...19892757371
[infectious endocarditis due to streptococcus bovis with suspected relationship with colon cancer]. 19892794665
[infectious endocarditis caused by streptococcus bovis and alcoholic cirrhosis complicated by hepatoma].the case is reported of a 66-year old man who developed streptococcus bovis endocarditis on a fairly loose aortic stenosis and who also presented with alcoholic cirrhosis complicated by an ultimately lethal hepatoma. on this occasion, comments are made on the following points: -str. bovis is increasingly responsible for bacterial endocarditis. this micro-organism is now rapidly and reliably identified. -str. bovis endocarditis has some clinical features of its own. -patients in whom the usual po ...19872823737
transport and phosphorylation of disaccharides by the ruminal bacterium streptococcus bovis.toluene-treated cells of streptococcus bovis jb1 phosphorylated cellobiose, glucose, maltose, and sucrose by the phosphoenolpyruvate-dependent phosphotransferase system. glucose phosphorylation was constitutive, while all three disaccharide systems were inducible. competition experiments indicated that separate phosphotransferase systems (enzymes ii) existed for glucose, maltose, and sucrose. [14c]maltose transport was inhibited by excess (10 mm) glucose and to a lesser extent by sucrose (90 and ...19872827569
streptococcus bovis bacteraemia and its association with alimentary-tract neoplasm. 19872880000
incidence of colonic lesions in streptococcus bovis and enterococcal endocarditis. 19872882164
carcinoma of the colon presenting as streptococcus equinus bacteremia. 19892910086
bacteremia with streptococcus bovis and streptococcus salivarius: clinical correlates of more accurate identification of isolates.two biotypes of streptococcus bovis can be identified by laboratory testing and can be distinguished from the phenotypically similar organism streptococcus salivarius. we assessed the clinical relevance of careful identification of these organisms in 68 patients with streptococcal bacteremia caused by these similar species. s. bovis was more likely to be clinically significant when isolated from blood (89%) than was s. salivarius (23%). there was a striking association between s. bovis i bactere ...19892915024
streptococcus bovis endocarditis and carcinoma of the colon. 19892924063
[right-left shunt in tricuspid endocarditis caused by streptococcus bovis]. 19862950416
[aneurysm of the ulnar artery in streptococcus bovis septicemia. first case]. 19872951723
fermentation of peptides and amino acids by a monensin-sensitive ruminal peptostreptococcus.a monensin-sensitive ruminal peptostreptococcus was able to grow rapidly (growth rate of 0.5/h) on an enzymatic hydrolysate of casein, but less than 23% of the amino acid nitrogen was ever utilized. when an acid hydrolysate was substituted for the enzymatic digest, more than 31% of the nitrogen was converted to ammonia and cell protein. coculture experiments and synergisms with peptide-degrading strains of bacteroides ruminicola and streptococcus bovis indicated that the peptostreptococcus was u ...19882975156
seasonal changes in the cecal microflora of the high-arctic svalbard reindeer (rangifer tarandus platyrhynchus).the dominant cecal bacteria in the high-arctic svalbard reindeer were characterized, their population densities were estimated, and cecal ph was determined in summer, when food quality and availability is good, and in winter, when it is very poor. in summer the total culturable viable bacterial population was (8.9 +/- 5.3) x 10(8) cells ml-1, whereas in winter it was (1.5 +/- 0.7) x 10(8) cells ml-1, representing a decrease to 17% of the summer population density. of the dominant species of cult ...19873030193
in vitro activity of ly146032 (daptomycin) against selected aerobic bacteria.the in vitro activity of the new lipopeptide antibiotic ly146032 was generally four-fold greater (mic 90 less than or equal to 0.5 microgram/ml) than that of vancomycin against methicillin-susceptible or methicillin-resistant staphylococcus aureus and coagulase-negative species of staphylococcus. enterococci, streptococcus bovis, group b and viridans streptococci, and corynebacterium group j-k isolates were inhibited by less than or equal to 4 micrograms/ml of ly146032, which represented activit ...19873032612
[endocarditis or bacteremia caused by streptococcus bovis and colorectal neoplasms].streptococcus bovis is a relatively frequent causative agent of endocarditis or bacteriaemia, particularly in the elderly. in the past s. bovis has often been incompletely or even falsely classified. for therapeutic and prognostic reasons it is important to classify this agent exactly with biochemical methods even in the routine laboratory. endocarditis or bacteriaemia due to s. bovis are often seen in conjunction with malignant, potentially malignant or benign colorectal neoplasias. after endoc ...19883045953
preservation of capsular material of streptococcal cells by specific lectins determined by immunoelectron microscopy.we describe the use of lectins as specific stabilizing agents for the polysaccharide capsular components of two gram-positive bacteria, streptococcus agalactiae and streptococcus bovis. treatment of bacterial suspensions with wheatgerm agglutinin and concanavalin a allowed better morphological preservation as well as immunoelectron microscopic localization of a capsular component (lipoteichoic acid) by employing specific antibodies and the protein a-gold technique. data obtained indicate that le ...19883071525
the bacteria responsible for ureolysis in artificial dental plaque.the origin of ureolytic activity in artificial-mouth plaques was established by assessing the contribution to plaque ureolytic activity of the isolated bacteria. to overcome losses of ureolytic activity caused by the unstable presence of urease in oral bacteria, ureolytic bacteria were isolated from an exceptionally active plaque (1 mumol nh3/min per mg protein) in which 63 per cent of the flora was ureolytic. after their ability to metabolize urea was stabilized, 13 ureolytic bacteria remained: ...19883075450
comparative survival of antibiotic-resistant and -sensitive fecal indicator bacteria in estuarine water.the survival of antibiotic-resistant and -sensitive strains of escherichia coli, enterococcus faecalis, enterococcus faecium, streptococcus equinus, and two environmental isolates, ap17 and aq62, was examined in estuarine water. each strain was rendered resistant to a combination of two antibiotics by serial passage in increasing concentrations of antibiotics. cultures were incubated in filter-sterilized estuarine water for up to 7 days. recovery was assessed by examining colony-forming ability ...19873111372
human choriogonadotropin-like material in bacteria of different species: electron microscopy and immunocytochemical studies with monoclonal and polyclonal antibodies.immunocytochemical studies using antisera to whole human choriogonadotropin (hcg), to its alpha- and beta-subunits and to the cooh-terminal peptide of hcg beta, and two monoclonal antibodies to hcg beta, demonstrated expression of hcg-like material, its individual subunits and/or fragments in nine bacterial strains. seven of these were isolated from patients with cancer and were definitely identified as streptococcus faecalis (three strains), staphylococcus haemolyticus (two strains) and staphyl ...19873116165
susceptibility and resistance of ruminal bacteria to antimicrobial feed additives.susceptibility and resistance of ruminal bacterial species to avoparcin, narasin, salinomycin, thiopeptin, tylosin, virginiamycin, and two new ionophore antibiotics, ro22-6924/004 and ro21-6447/009, were determined. generally, antimicrobial compounds were inhibitory to gram-positive bacteria and those bacteria that have gram-positive-like cell wall structure. mics ranged from 0.09 to 24.0 micrograms/ml. gram-negative bacteria were resistant at the highest concentration tested (48.0 micrograms/ml ...19873116929
factors affecting the rate of breakdown of bacterial protein in rumen fluid.1. the cellular proteins of butyrivibrio fibrisolvens, lactobacillus casei, megasphaera elsdenii, selenomonas ruminantium and streptococcus bovis were labelled by growth in the presence of l-[14c]leucine, and the breakdown of labelled protein was measured in incubations of these bacteria with rumen fluid to which unlabelled 5 mm-l-leucine was added. the rate of protein breakdown was estimated from the rate of release of radioactivity into acid-soluble material. 2. protein breakdown occurred at d ...19873118940
role of immunoblotting in the diagnosis of culture negative and enterococcal endocarditis.serum samples from patients with endocarditis and septicaemia due to enterococcus faecalis, enterococcus faecium, streptococcus bovis, and streptococcus sanguis were immunoblotted against antigenic extracts from all four species. in e faecalis endocarditis there was a strong igm response to e faecalis antigenic bands of 112, 88-90, and 45-47 kd and a strong igg response to 88-90 and 45-47 kd bands. in e faecium endocarditis there was a pronounced igg response to an e faecium band of 82-90 kd. fo ...19873119674
[use of the colonizing preparation amylastin in the stimulation of rumen-type digestion in young ruminants].in experiments with calves during the milk-nutrition period we tested the use of the colonisation preparation amylastim, containing a bacterial strain of streptococcus bovis ao 24/85, as a factor speeding up the development of the rumen microflora. we gave the preparation to the animals for a period of four weeks and studied its influence on the microflora adherent to the epithelium of the rumen wall. we ascertained a significant increase in the number of streptococcus bovis germs (p less than 0 ...19873127979
sodium-dependent transport of neutral amino acids by whole cells and membrane vesicles of streptococcus bovis, a ruminal bacterium.streptococcus bovis jb1 cells were able to transport serine, threonine, or alanine, but only when they were incubated in sodium buffers. if glucose-energized cells were washed in potassium phosphate and suspended in potassium phosphate buffer, there was no detectable uptake. cells deenergized with 2-deoxyglucose and incubated in sodium phosphate buffer were still able to transport serine, and this result indicated that the chemical sodium gradient was capable of driving transport. however, when ...19883136141
streptococcus bovis endocarditis associated with villous adenoma following colonoscopy. 19883177185
esterase activities in butyrivibrio fibrisolvens strains.thirty strains of butyrivibrio fibrisolvens isolated in diverse geographical locations were examined for esterase activity by using naphthyl esters of acetate, butyrate, caprylate, laurate, and palmitate. all strains possessed some esterase activity, and high levels of activity were observed with strains 49, h17c, s2, actf2, and lm8/1b. esterase activity also was detected in other ruminal bacteria (bacteroides ruminicola, selenomonas ruminantium, ruminobacter amylophilus, and streptococcus bovis ...19883178205
two-stage reimplantation in infected total knee arthroplasty.twenty-one infected total knee arthroplasties (tka) in 21 patients were treated from september 1980 through october 1987. of these, 15 were followed for more than one year. treatment of these patients consisted of thorough debridement of all infected tissue and components; a cement spacer was used in ten patients. the cement was impregnated with antibiotics. this procedure was followed for an average of 4.2 weeks with intravenous antibiotics and tka utilizing antibiotic-impregnated cement. five ...19883180576
streptococcus bovis bacteremia and digestive system neoplasms. 19883194717
partial characterisation of the inhibitory substances produced by streptococcus oralis and related species.the production of inhibitory substances was sought by deferred antagonism. streptococcus oralis, streptococcus mitis and streptococcus sanguis produced bactericidal concentrations of hydrogen peroxide, but no detectable bacteriocin. actinomycetes and neisseria pharyngis were particularly susceptible to the lethal action of hydrogen peroxide. streptococcus milleri produced bactericidal concentrations of hydrogen peroxide and bacteriocin-like substances. s. oralis strains were inhibited by the bac ...19883216798
effects of the ionophores monensin and tetronasin on simulated development of ruminal lactic acidosis in vitro.a continuous coculture of four ruminal bacteria, megasphaera elsdenii, selenomonas ruminantium, streptococcus bovis, and lactobacillus sp. strain lb17, was used to study the effects of the ionophores monensin and tetronasin on the changes in ruminal microbial ecology that occur during the onset of lactic acidosis. in control incubations, the system simulated the development of lactic acidosis in vivo, with an initial overgrowth of s. bovis when an excess of glucose was added to the fermentor. la ...19883223764
endocarditis due to streptococcus bovis--a case report. 19883249974
antimicrobial therapy of streptococcal endocarditis.the majority of patients with endocarditis caused by viridans streptococci or streptococcus bovis susceptible to less than or equal to 0.1 mg/l of penicillin may be treated successfully for two weeks with penicillin 20 x 10(6) million units intravenously together with streptomycin 7.5 mg/kg body weight intramuscularly twice daily or gentamicin 1 mg/kg body weight intravenously every 8 h. patients with nutritionally variant viridans streptococcal endocarditis should be treated for four weeks with ...19873316158
therapy of streptococcal endocarditis: correlation of animal model and clinical studies.streptococci (viridans group, streptococcus bovis, enterococci still account for the majority of cases of infective endocarditis in the non-addict population. experimental animal models of endocarditis have been used (primarily in rabbits) to delineate the major therapeutic principles of this disease; viz. bactericidal antimicrobial agents must be given parenterally in high dosages for prolonged period of time. overall, there is a good correlation between results obtained by: (1) in-vitro suscep ...19873316165
colonic villous adenoma, polyp, and leiomyoma presenting with streptococcus bovis endocarditis. 19883336976
differentiation of ruminal bacterial species by enzyme-linked immunosorbent assay using egg yolk antibodies from immunized chicken hens.cross-reactivity among four species of ruminal bacteria was examined by using egg yolk antibodies from immunized leghorn laying hens and an enzyme-linked-immunosorbent assay. the effects of the four species on the hens were compared on various days postimmunization. hens injected with the same bacterial species had similar apparent antibody levels over the entire postimmunization period, but only bacteroides ruminicola b1(4) and selenomonas ruminantium d antigens elicited early increases in appa ...19883355145
[streptococcus bovis bacteremia and colonic diseases].four febrile patients were found to have a bacteraemia or endocarditis caused by streptococcus bovis. although there were no gastrointestinal symptoms, polyps in the colon, of a high degree of malignancy, were discovered in three. in the fourth, carcinoma of the colon, requiring resection, was found seven years after streptococcus bovis endocarditis. the polyps were removed by endoscopy and the streptococcus bovis infection in all cases cured by penicillin. all four patients had accompanying res ...19883371220
susceptibilities of streptococcal strains associated with infective endocarditis to nine antibiotics.the susceptibilities of 121 streptococcal strains isolated from patients with infective endocarditis to mezlocillin, piperacillin, ceftizoxime, ceftriaxone, ceftazidime, imipenem, ciprofloxacin, ofloxacin and pefloxacin were determined by the agar dilution technique. viridans streptococci, streptococcus bovis and enterococcus faecalis were susceptible to imipenem, mezlocillin and piperacillin. all the strains, except e. faecalis were sensitive to ceftriaxone, ceftizoxime and ceftazidime and resi ...19883391051
effects of potassium ion concentrations on the antimicrobial activities of ionophores against ruminal anaerobes.the antimicrobial activities of monensin and lasalocid against representative strains of ruminal bacteria were evaluated in medium containing three different concentrations of potassium (1.3, 7.9, or 23.3 mm). the growth of eubacterium ruminantium was inhibited by low concentrations of ionophores (less than or equal to 0.16 mg/liter), while the strain of streptococcus bovis tested was resistant to high concentrations of ionophores (40 mg/liter) at all potassium concentrations tested. the mics of ...19873426214
interactions between rumen amylolytic and lactate-utilizing bacteria in growth on starch.the growth and metabolism of the rumen amylolytic bacteria streptococcus bovis, butyrivibrio fibrisolvens and bacteroides ruminicola, growing in pure cultures and co-cultures with the rumen lactilytic bacteria megasphaera elsdenii and veillonella alcalescens were followed. the interaction of amylolytic bacteria with v. alcalescens represents a simple food chain. the interaction with m. elsdenii is more complex, since there is a simultaneous competition for products of the starch degradation.19873429358
noma in an unusual case of perforated appendicitis with pneumoperitoneum.noma is a very rare gangrenous infection of the oral cavity usually associated with debilitating diseases, immunosuppression, or malnutrition. its development in a previously healthy 13-year-old boy with perforated appendicitis is presented. streptococcus bovis type ii and e coli were isolated from the noma and streptococcus bovis, bacteroides fragilis, and bacteroides asacharolyticus from the peritoneal fluid. a transient impaired immune cellular function was found and may have contributed to t ...19873430301
streptococcus bovis bacteraemia associated with candidal lesions of the large bowel in a leukaemic child. a case report.a 5-year-old boy with acute myeloblastic leukaemia had large-bowel masses, demonstrated at autopsy, 5 weeks after an episode of streptococcus bovis bacteraemia. the association of strept. bovis bacteraemia with large-bowel disease, well documented in adults, should be considered in children.19873474796
streptococcus bovis endocarditis and carcinoma of the colon. 19873504316
evaluation of aztreonam in the treatment of spontaneous bacterial peritonitis in patients with determine the efficacy of aztreonam in the treatment of spontaneous bacterial peritonitis in patients with hepatic cirrhosis, 14 patients (7 males, 7 females) with 16 gram-negative infective episodes (12 escherichia coli and 4 klebsiella pneumoniae) were treated with aztreonam infusions at doses of 1 gm per 8 hr for a planned 14-day period. ages ranged from 40 to 75 years with a mean of 57 +/- 10 years. all organisms were highly susceptible to aztreonam (minimal inhibitory concentration less ...19863530945
growth of gram-positive mastogenic bacteria in normal, simulated bulk tank, and mastitic milk held at simulated fluctuating temperatures of farm bulk tank.growth of staphylococcus aureus, staphylococcus epidermidis, streptococcus agalactiae, streptococcus dysgalactiae, streptococcus bovis, and streptococcus uberis was studied in normal milk, simulated bulk tank milk, and aseptic mastitic milk held at simulated fluctuating temperatures of farm bulk tank for 48 h. with the exception of s. bovis, growth rates of the other five bacteria were similar in both normal and simulated bulk tank milk. mastitic milk inhibited growth of all bacteria studied. a ...19863531259
[tricuspid endocarditis with right-left auricular shunt through a patent foramen ovale].the authors report the case of a tricuspid endocarditis secondary to streptococcus bovis with important regurgitation and severe hypoxemia secondary to a right-left atrial shunt through a patent foramen ovale, requiring a surgical treatment which included the replacement of the tricuspid valve and closure of the dehiscence in the inter-atrial septum. the presence of a patent foramen ovale in the course of a tricuspid endocarditis has been exceptionally reported. this diagnosis deserves to be evo ...19873548566
[endocarditis caused by streptococcus bovis and subjacent gastrointestinal disease]. 19863563296
ruminal microbial development in conventionally or early-weaned calves.eight bull calves (four holsteins and four ayrshire calves with mean birth weight of 38.8 kg) were ruminally cannulated at 3 d of age and allocated to one of two weaning programs. all calves were fed colostrum for 3 d after birth and milk until weaning. calves in the conventional-weaning program were fed a starter diet from 3 d of age and weaned at 6 wk of age. in the early-weaning program, calves were fed a highly palatable pre-starter diet from 3 d of age until they consumed 227 g/d, and then ...19873571026
lack of association of streptococcus bovis with noncolonic gastrointestinal carcinoma.since previous studies have shown an increased fecal carriage rate of streptococcus bovis in patients with carcinoma of the colon, a study was undertaken to determine the fecal carriage rate of this organism in patients with gastrointestinal carcinoma of noncolonic origin. organisms identified as s. bovis were isolated on fecal culture from 10 of 82 (12%) controls, 19 of 94 (20%) individuals with noncolonic gastrointestinal carcinoma, and 12 of 80 (15%) patients with other gastrointestinal disor ...19873578233
a proposed mechanism of monensin action in inhibiting ruminal bacterial growth: effects on ion flux and protonmotive force.streptococcus bovis, a gram-positive ruminal bacterium, was unable to grow in the presence of monensin. when monensin (5 mg/liter) was added to actively growing cultures, there was an immediate decrease in growth rate, and within 3 h no further growth was observed. glucose utilization and lactate production continued for another 8 h even though growth had ceased. monensin caused a decrease (p less than .05) in intracellular k+, a decrease (p less than .05) in intracellular ph and an increase (p ...19873583956
streptococcus bovis meningitis. 19873625223
infective endocarditis: clinical features in young and elderly patients.the elderly constitute an increasing percentage of patients with infective endocarditis. the disease manifestations and outcomes in 53 episodes of endocarditis in patients over the age of 60 were reviewed and compared with 55 episodes of endocarditis in patients less than 40 years of age and 46 episodes of endocarditis in patients aged 40 to 60. the percentage of cases caused by staphylococci and streptococci were roughly equal in all groups. enterococci, streptococcus bovis, and coagulase-negat ...19873674051
infective endocarditis due to penicillin-tolerant streptococcus bovis.two streptococcus bovis isolates obtained from patients with endocarditis were found to be tolerant to penicillin and other cell wall active agents. by time-kill analysis, penicillin and streptomycin acted synergistically against these strains. the existence of tolerant s. bovis strains should be considered when initially choosing antibiotics for the treatment of serious s. bovis infections.19863720266
antibodies to bacterial vaccines demonstrating specificity for human choriogonadotropin (hcg) and immunochemical detection of hcg-like factor in subcellular bacterial fractions.investigations were done to determine whether vaccines prepared with chemically killed staphylococcus haemolyticus ru1 and streptococcus bovis av46 (bacteria that have been demonstrated to express human choriogonadotropin [hcg]-like material on their surface) elicited antibodies in rabbits with specificity for hcg determinants. in addition, the anatomical locus of the hcg-like factor was determined by separation of bacterial subcellular fractions. the results demonstrated that these bacterial va ...19863721581
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