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re-identification of streptococcus bovis isolates causing bacteraemia according with the new taxonomy criteria: still an issue?all streptococcus bovis blood culture isolates recovered from jan-2003 to jan-2010 (n=52) in our institution were re-identified using new taxonomic criteria based upon their genetic traits. initial identification was performed by the semiautomatic wider system (fco. soria-melguizo, spain) and the api 20 strep (biomérieux, france). all isolates were re-identified by pcr amplification and sequencing of both 16s rdna and soda genes and by mass spectrometry using maldi-tof ms (bruker, germany). resu ...201121752968
[descriptive analysis of diseases associated with streptococcus bovis bacteremia. suggestion of a first protocol of study.]background and objective: it is well-known the relationship between streptococcus bovis (s. bovis) bacteremia and colon cancer, liver cirrhosis and others neoplasms. however, a study protocol to rule out these underlying diseases has not been carried out yet. our objective was to describe s. bovis bacteremia and associated diseases. patients and method: multicenter, retrospective cohort study. s. bovis bacteremias episodes between 2001 and 2009 were included. mean variables: colon neoplasm, non- ...201121719046
two weeks of postsurgical therapy may be enough for high-risk cases of endocarditis caused by streptococcus viridans or streptococcus bovis.clin microbiol infect abstract: the duration of antimicrobial therapy after surgery for infective endocarditis (ie) is controversial. a short course of postsurgical therapy is currently accepted only for patients with negative valve culture. we performed a retrospective (1994-2008) analysis of patients who underwent surgery for ie in our hospital and had a high risk of complications ( one of more of the following: <2-áweeks of antibiotic treatment before surgery; embolism; perivalvular extension ...201121733030
[diagnostic approach to streptococcus bovis bacteremia, or the limits of the evidence-based medicine.] 201121719047
regulation of lactate production in streptococcus bovis: a spiraling effect that contributes to rumen acidosis.when streptococcus bovis was grown in batch culture with 6 g/l glucose at ph 6.7, maximum specific growth rate was 1.47 h(-1), and lactate was the primary fermentation product. in continuous culture at ph 6.7 and growth rate equal to .10 h(-1), little lactate was formed, and formate, acetate, and ethanol accounted for most of the product. when extra-cellular ph decreased to 4.7, intra-cellular ph declined to 5.4, and organisms switched back to lactate production. intracellular concentration of f ...198521800456
efficacy and safety of outpatient parenteral antibiotic therapy for infective endocarditis: a ten-year prospective study.background: the length of treatment of infective endocarditis (ie) with parenteral antibiotics varies from 2 to 6 weeks. although several studies indicate that outpatient parenteral antibiotic treatment (opat) could be safe for uncomplicated viridans-group streptococci (vgs) ie, the experience in spain is limited and data on other types of endocarditis and opat are scarce worldwide. methods: prospective single center study of a cohort including all patients with ie admitted to the hospital clini ...201121723004
long-term gastrointestinal outcomes after streptococcus bovis bacteraemia. 201121995699
septic arthritis due to streptococcus bovis in a patient with liver cirrhosis due to hepatitis c virus: case report and literature review.monoarthritis remains a diagnostic challenge in rheumatology and orthopedics. the author reports a case of septic arthritis due to streptococcus bovis after several episodes of joint effusion treated with hyaluronic acid (hylan g-f 20) and methylprednisolone acetate in a 69-year-old patient with liver cirrhosis due to hepatitis c virus. neither adenoma of the colon nor endocarditis was present. the diagnostic possibilities for this case of monoarthritis, the pathologies related to the microorgan ...201121953003
streptococcus bovis infections, colorectal cancer and liver dysfunction. 201122023511
A Longitudinal Case Series Description of Meningitis Due to Streptococcus gallolyticus subsp. pasteurianus in Infants.Streptococcus gallolyticus subsp. pasteurianus, previously known as Streptococcus bovis biotype II.2, is known to cause multiple infectious complications, including bacterial meningitis, in adults. Only sporadic individual case reports have identified this pathogen as a cause of meningitis in infants. This study is the first to longitudinally document S. gallolyticus subsp. pasteurianus as a cause of meningitis in four epidemiologically unrelated infants less than 2 weeks of age. The 16S rRNA ge ...201222075594
A novel multiplex PCR/RFLP assay for the identification of Streptococcus bovis/Streptococcus equinus complex members from dairy microbial communities based on the 16S rRNA gene.The Streptococcus bovis/Streptococcus equinus complex (SBSEC) comprises pathogenic species associated with different degrees with human infections but also spontaneously fermented dairy products. We aimed therefore at developing a specific identification assay for the SBSEC targeting the 16S rRNA gene comprising a multiplex PCR followed by a differentiating restriction fragment length polymorphisms (RFLP). The multiplex PCR assay was positively applied on 200 SBSEC isolates including reference s ...201222092382
role of lipid ii and membrane thickness in the mechanism of action of the lantibiotic bovicin hc5.lantibiotics are antimicrobial peptides produced by gram-positive bacteria, nisin being the most well-known member. nisin inhibits peptidoglycan synthesis and forms pores at sensitive membranes upon interaction with lipid ii, the essential bacterial cell wall precursor. bovicin hc5, a bacteriocin produced by streptococcus bovis hc5, has the putative n-terminal lipid ii binding motif, and we investigated the mode of action of bovicin hc5 using both living bacteria and model membranes, with specia ...201121876041
infected aneurysm of brachial artery after mitral valve infective endocarditis.we present a case of brachial artery infected aneurysm in a patient with infective endocarditis caused by streptococcus bovis. a 49-year-old man presented with fever dyspnea and a pansystolic murmur with irradiation to axilla. the echocardiogram revealed vegetation in native mitral valve. after mitral valve replacement with bioprosthesis, it was observed pulsatile mass of five centimeters in diameter at antecubital fossa of right upper limb. it was made the diagnosis of infected aneurysm of the ...201121881723
streptococcus bovis bacteremia and colorectal carcinoma. 201121921348
significant association of streptococcus bovis with malignant gastrointestinal diseases.streptococcus bovis is a gram-positive bacterium causing serious human infections, including endocarditis and bacteremia, and is usually associated with underlying disease. the aims of the current study were to compare prevalence of the bacterium associated with malignant and nonmalignant gastrointestinal diseases and to determine the susceptibility of the isolated strains to different antimicrobial agents. the result showed that the prevalence of s. bovis in stool specimens from patients with m ...201122121365
clinical importance of streptococcus gallolyticus infection among colorectal cancer patients: systematic review and meta-analysis.streptococcus bovis has long been associated with colorectal cancer (crc). however, not all genospecies are as closely related to crc. with this systematic review, we aim to increase the awareness of the association between s. bovis biotype i (streptococcus gallolyticus) and crc and urge for uniform molecular microbiological classification.201121960713
No need to search for the source of haematogenous arthroplasty infections.QUESTIONS UNDER STUDY/PRINCIPLES: Prosthetic joint infections (PJI) may be a potential sentinel event for an unknown neoplastic or infectious source in elderly patients. However, the value and cost-effectiveness of investigations to determine the origin of these infections is unknown.201122180157
cow teat skin, a potential source of diverse microbial populations for cheese production.the diversity of the microbial community on cow teat skin was evaluated using a culture-dependent method based on the use of different dairy-specific media, followed by the identification of isolates by 16s rrna gene sequencing. this was combined with a direct molecular approach by cloning and 16s rrna gene sequencing. this study highlighted the large diversity of the bacterial community that may be found on teat skin, where 79.8% of clones corresponded to various unidentified species as well as ...201222081572
Selective Antibody Response to Streptococcus gallolyticus Pilus Proteins in Colorectal Cancer Patients.Streptococcus gallolyticus subsp. gallolyticus (previously called Streptococcus bovis biotype I) infections have long been associated with colorectal cancer (CRC). This work aimed to investigate the CRC-associated humoral immune response to four pilus proteins of this bacterium by newly developed ELISAs. Pilus proteins are interesting diagnostic targets as they are the building blocks of pilin-like structures that mediate bacterial virulence and are readily exposed to the host immune system upon ...201122012878
streptococcus bovis meningitis associated with colonic diverticulosis and hearing impairment: a case report.a 75-year-old woman presented to the tropical diseases hospital, goiania, brazil, with a two-day history of fever and chills followed by headache and vomiting over the last 24 hours. the cerebrospinal fluid (csf) showed 270 leukocytes/mmc (30 percent neutrophils); 20 red cells/mmc; undetectable levels of glucose and 232 mg/dl of protein. the gram stain revealed several gram-positive cocci, and csf culture yielded streptococcus bovis. a colonoscopy showed diverticula in descendent and transverse ...201122212167
the correlation between composition of the bacterial community and concentration of volatile fatty acids in the rumen during the transition period and ketosis in dairy cows.the transition period is a severe challenge to dairy cows. glucose supply cannot meet demand and body fat is mobilized, potentially leading to negative energy balance (neb), ketosis or fatty liver. propionate produces glucose by gluconeogenesis, which generation depends heavily on the number and species of microbes. in present study we analyzed the rumen microbiome composition of cows in the transition period, cows with ketosis and non-perinatal cows using terminal restriction fragment length po ...201222267666
effects of a combination of hinokitiol (β-thujaplicin) and an organic acid mixture on ruminal fermentation in heifers fed a high-grain diet.this study evaluated the effects of hinokitiol (a natural antibacterial compound extracted from thujopsis dolabrata var. hondai) and an organic acid mixture (citrate content 50%) on ruminal fermentation. antibacterial properties were examined by measuring minimal inhibitory concentration. hinokitiol at 1.56 µg/ml or an organic acid mixture at 1600 µg/ml inhibited streptococcus bovis growth. the combination of 0.78 µg/ml hinokitiol and 200 µg/ml of an organic acid mixture also inhibited s. bovis ...201222250737
effect of lactobacillus casei on streptococcus bovis in faecal flora.bacteraemia caused by streptococcus bovis is often associated with colorectal tumours. also, experimental studies have been proposed that s bovis acts as a promoter of tumours. we report the case of a man with colon adenoma who had a high proportion of s bovis in his faecal flora. he was treated with a lactobacillus casei preparation (blp) and the effect on the faecal flora was examined. l casei reduced the proportion of s bovis (from 43% down to 9%), and the effect continued after the administr ...201022242064
infective endocarditis due to streptococcus gallolyticus associated with colonic displasia.streptococcus gallolyticus is a microorganism belonging to the streptococcus bovis i group isolated in humans, bovines and equines pigeons, among other animals. streptococcus bovis is a streptococcus strain found in the rumen, and has been isolated in the milk of animals with mastitis. the authors describe a case of an adult immunocompetent patient with underlying valvular heart disease, with bacteraemia and infective endocarditis by streptococcus gallolyticus, in whom adenomatous colonic polyps ...201122521029
[streptococcus bovis endocarditis that presented a difficulty in differentiating from urinary tract infection and collagen disease]. 201122479729
streptococcus bovis isolated in haemoculture a signal of malignant lesion of the colon.s. bovis is known for causing bacteremia and endocarditis as well as accompanying malignant diseases of the gastrointestinal tract. hence, identification of this species and recognition of the clinical characteristics of infections caused are essential for both therapy and prognosis. s. bovis isolated from haemoculture requires an immediate search for gastrointestinal tract lesions.201122239034
how a bowel tumour led to meningitis.this report summarises a case history in which a 75-year-old man was admitted with bacterial meningitis caused by streptococcus bovis. a subsequent abdominal investigation in the light of the known association between invasive s bovis infection and colonic cancer confirmed the suspicion of a cancer located to the rectum. the patient died from clostridium difficile enterocolitis with sepsis and multi-organ failure. this is a highly relevant and interesting case presentation, which besides providi ...201122692778
getting to the heart of rectal bleeding: subacute bacterial endocarditis presenting as anaemia and a gi bleed.in this case report, the authors demonstrate a case of subacute bacterial endocarditis presenting with anaemia. it is the first of its kind to describe a delay in diagnosis due to an initial patient investigation for a bleed rather than a cardiac evaluation. astute clinicians need to be aware of the causes of anaemia in patients with endocarditis and consider that in streptococcus bovis (s bovis) infection can be related to gastrointestinal polyps or malignancy resulting in bleeding. although pa ...201122674949
particularities in diagnosis and treatment for infectious endocarditis in children.infectious endocarditis (ie) represents a rare pathology in children, but with lethal potential. the goal of the therapy is fast and total eradication of the infection.201223700883
streptococcus gallolyticus subspecies pasteurianus meningitis in an infant: a case report and literature review.a case of 6-week-old male infant with meningitis and concurrent bacteremia caused by streptococcus gallolyticus subspecies pasteurianus (streptococcus bovis biotype 11.2) is presented. the isolates were susceptible to all beta-lactam antibiotics. nevertheless, delayed defervescence and lack of satisfactory clinical improvement after treatment with multiple beta-lactam antibiotics prompted the combination therapy of vancomycin and penicillin g. the patient completed a 2-week course of antibiotics ...201223390793
dynamics of fecal microbial communities in children with diarrhea of unknown etiology and genomic analysis of associated streptococcus lutetiensis.the sequences of the 16s rrna genes extracted from fecal samples provide insights into the dynamics of fecal microflora. this potentially gives valuable etiological information for patients whose conditions have been ascribed to unknown pathogens, which cannot be accomplished using routine culture methods. we studied 33 children with diarrhea who were admitted to the children's hospital in shanxi province during 2006.201323782707
suicin 3908, a new lantibiotic produced by a strain of streptococcus suis serotype 2 isolated from a healthy carrier pig.while streptococcus suis serotype 2 is known to cause severe infections in pigs, it can also be isolated from the tonsils of healthy animals that do not develop infections. we hypothesized that s. suis strains in healthy carrier pigs may have the ability to produce bacteriocins, which may contribute to preventing infections by pathogenic s. suis strains. two of ten s. suis serotype 2 strains isolated from healthy carrier pigs exhibited antibacterial activity against pathogenic s. suis isolates. ...201525659110
oral and airway microbiota in hiv-infected pneumonia patients.despite the increased frequency of recurrent pneumonia in hiv-infected patients and recent studies linking the airway bacterial community (microbiota) to acute and chronic respiratory infection, little is known of the oral and airway microbiota that exist in these individuals and their propensity to harbor pathogens despite antimicrobial treatment for acute pneumonia. this pilot study compared paired samples of the oral and airway microbiota from 15 hospitalized hiv-infected patients receiving a ...201222760045
direct production of organic acids from starch by cell surface-engineered corynebacterium glutamicum in anaerobic conditions.we produced organic acids, including lactate and succinate, directly from soluble starch under anaerobic conditions using high cell-density cultures of corynebacterium glutamicum displaying α-amylase (amya) from streptococcus bovis 148 on the cell surface. notably, reactions performed under anaerobic conditions at 35 and 40°c, which are higher than the optimal growth temperature of 30°c, showed 32% and 19%, respectively, higher productivity of the organic acids lactate, succinate, and acetate co ...201324342107
evaluation of the nanosphere verigene gram-positive blood culture assay with the versatrek blood culture system and assessment of possible impact on selected patients.the verigene gram-positive blood culture (bc-gp) assay (nanosphere, northbrook, il) is a molecular method for the rapid identification of gram-positive organisms and resistance markers directly from blood culture bottles. a total of 148 versatrek redox 1 40-ml aerobic bottles demonstrating gram-positive bacteria were tested. results were compared with those from conventional biochemical and matrix-assisted laser desorption ionization-time of flight (maldi-tof) identifications. we obtained isolat ...201324048531
[significance of bacteria detection with filter paper method on diagnosis of diabetic foot wound infection].objective: to evaluate the significance of bacteria detection with filter paper method on diagnosis of diabetic foot wound infection. methods: eighteen patients with diabetic foot ulcer conforming to the study criteria were hospitalized in liyuan hospital affiliated to tongji medical college of huazhong university of science and technology from july 2014 to july 2015. diabetic foot ulcer wounds were classified according to the university of texas diabetic foot classification (hereinafter referre ...201728219141
high concentration of red clay as an alternative for antibiotics in aquaculture.the use of antibiotics in aquaculture raises environmental and food safety concerns because chronic exposure of an aquatic ecosystem to antibiotics can result in the spread of antibiotic resistance, bioaccumulation of antibiotics in the organisms, and transfer of antibiotics to humans. in an attempt to overcome these problems, high-concentration red clay was applied as an alternative antibiotic against the following common fish pathogens: aeromonas salmonicida, vibrio alginolyticus, and streptoc ...201626528535
challenges in the production of itaconic acid by metabolically engineered escherichia coli.metabolic engineering allows the production of a variety of high-value chemicals in heterologous hosts. for example, itaconic acid (ia) has been produced in several microorganisms, such as escherichia coli, aspergillus niger, and synechocystis sp. through the expression of cis-aconitate decarboxylase gene (cad) from aspergillus terreus. recently, we showed that inactivation of the isocitrate dehydrogenase gene and overexpression of the aconitase gene dramatically enhanced the production levels o ...201526176321
production of itaconic acid in escherichia coli expressing recombinant α-amylase using starch as substrate.several studies on fermentative production of a vinyl monomer itaconic acid from hydrolyzed starch using aspergillus terreus have been reported. herein, we report itaconic acid production by escherichia coli expressing recombinant α-amylase, using soluble starch as its sole carbon source. to express α-amylase in e. coli, we first constructed recombinant plasmids expressing α-amylases by using cell surface display technology derived from two amylolytic bacteria, bacillus amyloliquefaciens nbrc 15 ...201525468427
bovicin hj50-like lantibiotics, a novel subgroup of lantibiotics featured by an indispensable disulfide bridge.lantibiotics are ribosomally-synthesized and posttranslationally modified peptides with potent antimicrobial activities. discovery of novel lantibiotics has been greatly accelerated with the soaring release of genomic information of microorganisms. as a unique class ii lantibiotic, bovicin hj50 is produced by streptococcus bovis hj50 and contains one rare disulfide bridge. by using its precursor bova as a drive sequence, 16 bova-like peptides were revealed in a wide variety of species. from them ...201424821187
molecular characterization of α-amylase from staphylococcus aureus.staphylococcus aureus is one of the prominent gram positive human pathogen secretes many surface and secretary proteins including various enzymes and pathogenic factors that favour the successful colonization and infection of host tissue. α-amylase is one of the enzymes secreted by s. aureus which catalyses the breakdown of complex sugars to monosaccharides, which are required for colonization and survival of this pathogen in any anatomical locales. in the present study we have cloned, sequenced ...201323559746
lipopeptide biosurfactants from paenibacillus polymyxa inhibit single and mixed species biofilms.although biofilms are recognised as important in microbial colonisation, solutions to their inhibition are predominantly based on planktonic assays. these solutions have limited efficacy against biofilms. here, a series of biofilm-orientated tests were used to identify anti-biofilm compounds from marine micro-flora. this led to the isolation of a complex of anti-biofilm compounds from an extract of paenibacillus polymyxa (ppe). a combination of rphplc and mass spectrometry identified the princip ...201223113815
gut microbiota imbalance and colorectal cancer.the gut microbiota acts as a real organ. the symbiotic interactions between resident micro-organisms and the digestive tract highly contribute to maintain the gut homeostasis. however, alterations to the microbiome caused by environmental changes (e.g., infection, diet and/or lifestyle) can disturb this symbiotic relationship and promote disease, such as inflammatory bowel diseases and cancer. colorectal cancer is a complex association of tumoral cells, non-neoplastic cells and a large amount of ...201626811603
[role of infectious agents in the emergence of malignant tumors].according to the data of the international agency for research on cancer (iarc), at least 6 virus species (hpv, ebv, hhv-8/kshv, htlv-1, hbv, hcv), 4 helminthes species (schistosoma haematobium and japonicum, opisthorchis viverrini, clonorchis sinensis) and i bacterium species (helicobacter pylori) have been proved to be capable of causing the development of cancer. the analysis of the data available shows that merkel cell polyomavirus (mcv), herpes simplex virus (hsv), john cunningham polyomavi ...201223163048
proteolytic activity by multiple bacterial species isolated from chronic venous leg ulcers degrades matrix substrates.a major feature of chronic wounds is the loss of tissue, with the exposure of dermal components preventing primary closure and leading to bacterial colonization. bacterial colonization has been proposed as one of the common underlying pathologies present in chronic wounds. the objective of this exploratory study was to identify bacteria cultured from chronic venous leg ulcers and test for proteolytic activity that degrades matrix substrates.201323118301
photoinactivation of eight health-relevant bacterial species: determining the importance of the exogenous indirect mechanism.it is presently unknown to what extent the endogenous direct, endogenous indirect, and exogenous indirect mechanisms contribute to bacterial photoinactivation in natural surface waters. in this study, we investigated the importance of the exogenous indirect mechanism by conducting photoinactivation experiments with eight health-relevant bacterial species (bacteroides thetaiotaomicron, campylobacter jejuni, enterococcus faecalis, escherichia coli k12, e. coli o157:h7, salmonella enterica serovar ...201627121126
in situ identification and quantification of starch-hydrolyzing bacteria attached to barley and corn grain in the rumen of cows fed barley-based diets.cereal grains rich in starch are widely used to meet the energy demands of high-producing beef and dairy cattle. bacteria are important players in starch digestion in the rumen, and thus play an important role in the hydrolysis and fermentation of cereal grains. however, our understanding of the composition of the rumen starch-hydrolyzing bacteria (shb) is limited. in this study, bodipy fl dq starch staining combined with fluorescence in situ hybridization (fish) and quantitative fish were appli ...201526142428
rapid changes in key ruminal microbial populations during the induction of and recovery from diet-induced milk fat depression in dairy cows.the ruminant provides a powerful model for understanding the temporal dynamics of gastrointestinal microbial communities. diet-induced milk fat depression (mfd) in the dairy cow is caused by rumen-derived bioactive fatty acids, and is commonly attributed to the changes in the microbial population. the aim of the present study was to determine the changes occurring in nine ruminal bacterial taxa with well-characterised functions, and abundance of total fungi, ciliate protozoa and bacteria during ...201526123320
antimicrobial activity of brazilian propolis extracts against rumen bacteria in vitro.the antimicrobial activity of three brazilian propolis extracts was evaluated on bacterial strains representing major rumen functional groups. the extracts were prepared using different concentrations of propolis and alcohol, resulting in different phenolic compositions. the propolis extracts inhibited the growth of fibrobacter succinogenes s85, ruminococcus flavefaciens fd-1, ruminococcus albus 7, butyrivibrio fibrisolvens d1, prevotella albensis m384, peptostreptococcus sp. d1, clostridium ami ...201323653262
effects of soybean small peptides on rumen fermentation and on intestinal and total tract digestion of luxi yellow cattle.four luxi beef cattle (400±10 kg) fitted with ruminal, duodenal and ileal cannulas were used in a 4×4 latin square to assess the effects of soybean small peptide (ssp) infusion on rumen fermentation, diet digestion and flow of nutrient in the gastrointestinal tract. the ruminal infusion of ssp was 0 (control), 100, 200 and 300 g/d. ruminal ssp infusion linearly (p<0.01) and quadratically (p<0.01) increased microbial protein synthesis and rumen ammonia-n concentration. concentrations of total vol ...201325049708
antimicrobial constituents and synergism effect of the essential oils from cymbopogon citratus and alpinia galanga.from the fresh leaf sheathes of lemongrass (cymbopogon citratus) and rhizomes of galanga (alpinia galanga) light yellow and colorless oils, respectively, were obtained by hydrodistillation and microwave assisted extraction (mae) in yields of 0.24% and 0.03%, and 0.11% and trace (w/w), respectively. by gc/ms analysis, five major constituents were identified in lemongrass oil, e-citral, z-citral, beta-myrcene, selina-6-en-4-ol, and cis-ocimene, and five in galanga oil, 1,8-cineole, phenol 4-(2-pro ...201424689310
combined computed tomography and fluorodeoxyglucose positron emission tomography in the diagnosis of prosthetic valve endocarditis: a case series.the diagnosis of prosthetic valve endocarditis is challenging. the gold standard for prosthetic valve endocarditis diagnosis is trans-esophageal echocardiography. however, trans-esophageal echocardiography may result in negative findings or yield images difficult to differentiate from thrombus in patients with prosthetic valve endocarditis. combined computed tomography and fluorodeoxyglucose positron emission tomography is a potentially promising diagnostic tool for several infectious conditions ...201424418206
[recurrent meningitis due to anatomical defects: the bacteria indicates its origin].recurrent meningitis is a rare disease. anatomical abnormalities and immunodeficiency states are predisposing factors. four cases, in which immunodeficiency was excluded, are presented. the causal microorganism led to the detection of the anatomical defect responsible for the recurrences.201525446794
macedovicin, the second food-grade lantibiotic produced by streptococcus macedonicus aca-dc 198.streptococcus macedonicus aca-dc 198 was found to produce a second lantibiotic named macedovicin in addition to macedocin. macedovicin was purified to homogeneity and mass spectrometric analysis identified a peptide of approximately 3.4 kda. partial n-terminal sequence analysis and tandem mass spectrometry revealed that macedovicin was identical to bovicin hj50 and thermophilin 1277 produced by streptococcus bovis and streptococcus thermophilus, respectively. macedovicin inhibits a broad spectru ...201323122510
colorectal neoplasm in cases of clostridium septicum and streptococcus gallolyticus subsp. gallolyticus bacteraemia.bacteremia with clostridium septicum (cs) and streptococcus gallolyticus subsp. gallolyticus (sgg) have both been associated with colorectal neoplasms (crn) and colonoscopic examination is advised, however the differences and similarities in colorectal findings are not well known.201728236516
anaerobic bioremediation of rdx by ovine whole rumen fluid and pure culture isolates.the ability of ruminal microbes to degrade the explosive compound hexahydro-1,3,5-trinitro-1,3,5-triazine (rdx) in ovine whole rumen fluid (wrf) and as 24 bacterial isolates was examined under anaerobic conditions. compound degradation was monitored by high-performance liquid chromatography analysis, followed by liquid chromatography-tandem mass spectrometry identification of metabolites. organisms in wrf microcosms degraded 180 μm rdx within 4 h. nitroso-intermediates hexahydro-1-nitroso-3,5-di ...201322688904
is there a need for bacterial endocarditis prophylaxis in patients undergoing gastrointestinal endoscopy?heart valve repair or replacement is a serious problem. patients can benefit from an open dialogue between both cardiologists and gastroenterologists for the optimal effective patients care. the focused update on infective endocarditis of the american college of cardiology/american heart association 2008 (acc/aha guidelines) and guidelines on the prevention, diagnosis, and treatment of infective endocarditis (new version 2009) of the european society of cardiology (esc guidelines) describe proph ...201424566725
relationship between enterococcus faecalis infective endocarditis and colorectal neoplasm: preliminary results from a cohort of 154 patients.the association between streptococcus bovis group infective endocarditis and colorectal neoplasm (crn) is well-known. however, no studies have assessed the association between enterococcus faecalis infective endocarditis (efie) and crn. we aimed to determine whether the prevalence of crn is higher in patients with efie and an unclear source of infection than in patients with efie and a known source of infection or in the general population.201727916708
effect of starch source (corn, oats or wheat) and concentration on fermentation by equine faecal microbiota in vitro.the goal was to determine the effect of starch source (corn, oats and wheat) and concentration on: (i) total amylolytic bacteria, group d gram-positive cocci (gpc), lactobacilli and lactate-utilizing bacteria, and (ii) fermentation by equine microbiota.201526255645
two cases of proteinase 3-anti-neutrophil cytoplasmic antibody (pr3-anca)-related nephritis in infectious endocarditis.we herein report two cases of proteinase 3-anti-neutrophil cytoplasmic antibody (pr3-anca)-related nephritis in infectious endocarditis. in both cases, the patients were middle-aged men with proteinuria and hematuria, hypoalbuminemia, decreased kidney function, anemia, elevated c-reactive protein (crp) levels, and pr3-anca positivity. each had bacteremia, due to enterococcus faecium in one and streptococcus bovis in the other. one patient received aortic valve replacement therapy for aortic regu ...201727904114
chemical composition and nutrient degradability in elephant grass silage inoculated with streptococcus bovis isolated from the rumen.the objective of the present study was to assess the chemical and bromatological composition and in situ degradability of elephant grass silages inoculated with streptococcus bovis isolated from cattle rumen. a complete randomized design was used with four treatments and six replications: elephant grass silage, elephant grass silage inoculated with 10(6) cfu/g streptococcus bovis jb1 strains; elephant grass silage inoculated with 106 cfu/g streptococcus bovis hc5 strains; elephant grass silage i ...201424519006
comparison of nitroethane, 2-nitro-1-propanol, lauric acid, lauricidin® and the hawaiian marine algae, chaetoceros, for potential broad-spectrum control of anaerobically grown lactic acid bacteria.the gastrointestinal tract of bovines often contains bacteria that contribute to disorders of the rumen, and may also contain foodborne or opportunistic human pathogens as well as bacteria capable of causing mastitis in cows. thus there is a need to develop broad-spectrum therapies that are effective while not leading to unacceptably long antibiotic withdrawal times. the effects of the ch(4)-inhibitors nitroethane (2 mg/ml), 2-nitro-1-propanol (2 mg/ml), lauric acid (5 mg/ml), the commercial pro ...201222428888
nutritional models of experimentally-induced subacute ruminal acidosis (sara) differ in their impact on rumen and hindgut bacterial communities in dairy cows.effects of subacute ruminal acidosis (sara) challenges on the bacteria in rumen fluid, cecal digesta, and feces of dairy cows were determined using 16s rrna gene pyrosequencing and real-time quantitative pcr. six non-lactating holstein cows with cannulas in the rumen and cecum were used in a 3 × 3 latin square arrangement of treatments. during the first 3 wk of each experimental period, cows received a control diet containing 70% forages on a dry matter (dm) basis. in wk 4 of each period, cows r ...201628179895
cloning and characterization of the glutamate dehydrogenase gene in streptococcus bovis.streptococcus bovis, an etiologic agent of rumen acidosis in cattle, is a rumen bacterium that can grow in a chemically defined medium containing ammonia as a sole source of nitrogen. to understand its ability to assimilate inorganic ammonia, we focused on the function of glutamate dehydrogenase. in order to identify the gene encoding this enzyme, we first amplified an internal region of the gene by using degenerate primers corresponding to hexameric family i and nad(p)(+) binding motifs. subseq ...201627878891
transport of a fluorescent analogue of glucose (2-nbdg) versus radiolabeled sugars by rumen bacteria and escherichia coli.fluorescent tracers have been used to measure solute transport, but transport kinetics have not been evaluated by comparison of radiolabeled tracers. using streptococcus equinus jb1 and other bacteria, the objective of this study was to determine if a fluorescent analogue of glucose (2-nbdg) would be transported with the same kinetics and transporters as [(14)c]glucose. we uniquely modified a technique for measuring transport of radiolabeled tracers so that transport of a fluorescent tracer (2-n ...201627096355
five cases of recurrent meningitis associated with chronic strongyloidiasis.although meningitis secondary to chronic strongyloidiasis is a rare complication, it is associated with a high mortality rate. recurrent meningitis can occur if the underlying parasitic infection is left untreated. we report five cases of recurrent meningitis related to chronic strongyloidiasis that were associated with human t-lymphotropic virus type 1 (htlv-1) infection. common causative organisms are escherichia coli, streptococcus bovis, and klebsiella pneumonia. one patient died during the ...201525548379
cloning, expression, and purification of a new antibacterial substance gene from larvae of musca domestica (diptera: muscidae).musca domestica l. (diptera: muscidae), the housefly, exhibits unique immune defenses and can produce antibacterial substances upon stimulation with bacteria. on the basis of the cdna library constructed using the suppression subtractive hybridization method, a 1188-bp antibacterial substance gene, which we named as566, was amplified by rapid amplification of cdna ends from m. domestica larva stimulated with salmonella pullorum (enterobacteriaceae: salmonella). in this study, the full-length as5 ...201425434038
l-lactate production from seaweed hydrolysate of laminaria japonica using metabolically engineered escherichia coli.renewable and carbon neutral, marine algal biomass could be an attractive alternative substrate for the production of biofuel and various biorefinery products. thus, the feasibility of brown seaweed (laminaria japonica) hydrolysate as a carbon source was investigated here for l-lactate production. this work reports the homofermentative route for l-lactate production by introducing streptococcus bovis/equinus l-lactate dehydrogenase in an engineered escherichia coli strain where synthesis of the ...201424297185
[progress of research in the relationship between microorganisms and colorectal cancer].colorectal cancer is a common carcinoma of gastrointestinal tract, and its incidence is associated with genetic mutations, environment as well as inflammation. recent studies have shown that many microorganisms may have played an important role in pathogenesis of colorectal cancer. the common bacteria involved in colorectal cancer are streptococcus bovis, helicobacter pylori, escherichia coli, bacteroides, and fusobacterium, etc. the common viruses are polyomavirus, epstein barr virus, cytomegal ...201324277413
streptococcus bovis-related cholecystitis.acute cholecystitis is a common inflammatory condition of the gallbladder caused most commonly by escherichia coli, enterococcus, and klebsiella organisms. streptococcus bovis is a gram-positive, catalase-negative, anaerobic coccus found as a commensal inhabitant of the digestive system in 16% of healthy people. we report a rare case of acute cholecystitis caused by s bovis and discuss its implications with regard to the two known s bovis biotypes (i & ii) both of which are associated with a num ...201323605823
relationship between concentration of lingual antimicrobial peptide and somatic cell count in milk of dairy cows.lingual antimicrobial peptide (lap) belongs to the β-defensin family in cattle and is found in milk. lap concentrations increase in milk from mastitic udders; however, the relationship between lap concentrations and the somatic cell count (scc) in milk remains to be elucidated in detail. therefore, the present study was undertaken to investigate the relationship between lap concentrations and the scc in bovine milk to assess whether lap may be used as an indicator of scc. milk was collected from ...201323528609
occurrence of bacteriuria in 18 catheterised cats with obstructive lower urinary tract disease: a pilot study.the incidence of catheter-associated urinary tract infections in cats catheterised for an obstructive lower urinary tract disease (lutd) has not previously been evaluated. the objective of this study was to evaluate the frequency of significant bacteriuria in cats with obstructive lutd managed for 48 h with a closed urine collection system. eighteen male cats admitted for a non-infectious obstructive lutd were evaluated. this was a prospective study. a standard protocol was used for aseptic cath ...201323400682
efficient synthesis of l-lactic acid from glycerol by metabolically engineered escherichia coli.due to its abundance and low-price, glycerol has become an attractive carbon source for the industrial production of value-added fuels and chemicals. this work reports the engineering of e. coli for the efficient conversion of glycerol into l-lactic acid (l-lactate).201323347598
early onset neonatal bacterial meningitis caused by streptococcus gallolyticus subsp. paste urianus.we report a case of neonatal meningitis due to streptococcus gallolyticus subsp. pasteurianus born to a mother with an asymptomatic urinary tract infection due to streptococcus group d and escherichia coli. in the past, this organism may have been reported as streptococcus bovis or s. bovis biotype ii/2. accurate identification of this organism is necessary to determine the etiology of infection and give correct treatment of neonatal meningitis, caused by this organism.201223082565
molecular characterization of a novel mosaic tet(s/m) gene encoding tetracycline resistance in foodborne strains of streptococcus bovis.the presence of antibiotic-resistance (ar) genes in foodborne bacteria of enteric origin represents a relevant threat to human health in the case of opportunistic pathogens, which can reach the human gut through the food chain. streptococcus bovis is a human opportunistic pathogen often associated with infections in immune-compromised or cancer patients, and it can also be detected in the environment, including fermented foods. we have focused on the molecular characterization of a tetracycline ...201222723288
catalytic efficiency diversification of duplicate β-1,3-1,4-glucanases from neocallimastix patriciarum j11.four types of β-1,3-1,4 glucanase (β-glucanase, ec 3.2.1.73) genes, designated bgla13, bgla16, bgla51, and bglm2, were found in the cdna library of neocallimastix patriciarum j11. all were highly homologous with each other and demonstrated a close phylogenetic relationship with and a similar codon bias to streptococcus equinus. the presence of expansion and several predicted secondary structures in the 3' untranslated regions (3'utrs) of bgla16 and bglm2 suggest that these two genes were duplica ...201222492445
effect of sugarcane fiber digestibility, conservation method and concentrate level on the ruminal ecosystem of beef cattle.the aim of this study was to investigate the effects of sugarcane neutral detergent fiber digestibility (ndfd), conservation method, and concentrate level on the ruminal microbial population of steers. eight ruminal-cannulated nellore steers were distributed in two contemporary 4 × 4 latin square design with a 2 × 2 factorial arrangement of treatments. experiment 1: diets were formulated with 60% of concentrate level, and two sugarcane genotypes (high or low ndfd) either freshly cut or as silage ...201728265975
effects of the dose and viability of saccharomyces cerevisiae. 1. diversity of ruminal microbes as analyzed by illumina miseq sequencing and quantitative pcr.this study was conducted to examine effects of the dose and viability of supplemental saccharomyces cerevisiae on the ruminal fermentation and bacteria population and the performance of lactating dairy cows. four ruminally cannulated lactating cows averaging 284±18d in milk were assigned to 4 treatments arranged in a 4×4 latin square design with four 21-d periods. cows were fed a total mixed ration containing 41.7% corn silage, 12.1% brewer's grains, and 46.2% concentrate on a dry matter basis. ...201727837973
effect of fiber digestibility and conservation method on feed intake and the ruminal ecosystem of growing steers.fiber digestibility is an important factor regulating dmi in ruminants. additionally, the ensiling process can also affect digestibility and chemical composition of the forage. the objective of this study was to investigate effects of sugarcane ndf digestibility (ndfd) and conservation method on intake, rumen kinetics, and the ruminal ecosystem of steers. eight ruminally cannulated nellore steers (275±22 kg bw) were used in a replicated 4×4 latin square design with a 2×2 factorial arrangement of ...201425414108
active dry saccharomyces cerevisiae can alleviate the effect of subacute ruminal acidosis in lactating dairy cows.the objective of the study was to determine the effect of active dry saccharomyces cerevisiae (adsc) supplementation on dry matter intake, milk yield, milk components, ruminal ph, and microbial community during a dietary regimen that leads to subacute ruminal acidosis (sara). sixteen multiparous, rumen-cannulated lactating holstein cows were randomly assigned to 1 of 2 dietary treatments that included adsc (biomate; ab vista, marlborough, uk; 8 × 10(10) cfu/head per day) or control. during wk 1 ...201425282426
the effects of active dried and killed dried yeast on subacute ruminal acidosis, ruminal fermentation, and nutrient digestibility in beef heifers.the study addressed the importance of yeast (saccharomyces cerevisiae) viability for reducing the incidence of subacute ruminal acidosis (sara) and improving total tract nutrient digestibility in beef heifers. six ruminally cannulated beef heifers (680 ± 50 kg bw) were used in a replicated 3 × 3 latin square design and were fed a diet consisting of 40% barley silage, 10% chopped grass hay, and 50% barley grain-based concentrate (dm basis). treatments were 1) no yeast (control), 2) active dried y ...201424398831
use of real-time pcr technique in determination of major fibrolytic and non fibrolytic bacteria present in indian surti buffaloes (bubalus bubalis).in the milk industry in india, buffalo breeds are most commonly used for milk production. efficiency of fiber digestion in ruminants is critical for animal productivity. bacteria play an important role in fiber digestion and utilization. absolute quantification real-time pcr was used to quantify ten bacterial species in rumen fluid of surti buffalo fed green fodder, dry roughage and compound concentrate mixture. abundance of each target taxon was calculated as a fraction of the total 16s rrna ge ...201324053023
effects of hop varieties on ruminal fermentation and bacterial community in an artificial rumen (rusitec).there is a growing interest in the use of hops (humulus lupulus) as an alternative to antibiotics to manipulate ruminal fermentation. however, the effects of different hop varieties on ruminal fermentation and bacterial populations have not been studied. here the effects of three hop varieties, cascade (cas), millennium (mil) and teamaker (tm), at a level of 800 µg ml(-1) inoculum on ruminal fermentation and microbial populations in an artificial rumen system (rusitec) fed a barley silage-based ...201322692875
a fibrolytic enzyme additive for lactating holstein cow diets: ruminal fermentation, rumen microbial populations, and enteric methane emissions.the objective was to determine if supplementing a dairy cow diet with an exogenous fibrolytic enzyme additive (econase rde; ab vista, marlborough, wiltshire, uk) altered fermentation, ph, and microbial populations in the rumen or enteric methane (ch(4)) emissions. in a companion study, this enzyme additive improved efficiency of fat-corrected milk production in a dose-dependent manner by up to 11% for early lactation dairy cows. nine ruminally cannulated, lactating holstein cows were used in a r ...201222365224
[endocarditis caused by rare gram-positive bacteria: investigate for gastrointestinal disorders].gemella haemolysans, streptococcus equinus and tropheryma whipplei are rare gram-positive bacteria which may cause endocarditis and are associated with gastrointestinal disorders. we report on three patients with infective endocarditis caused by these microorganisms. g. haemolysans and s. equinus (streptococcus bovis group) were isolated from blood cultures, and t. whipplei was diagnosed by molecular typing of an excised heart valve. the association between endocarditis caused by these microorga ...201222607842
meningitis associated with strongyloidiasis in an area endemic for strongyloidiasis and human t-lymphotropic virus-1: a single-center experience in japan between 1990 and 2010.meningitis caused by enteric flora is a known complication of strongyloidiasis, and human t-lymphotropic virus-1 (htlv-1) predisposes individuals to severe strongyloidiasis. we reviewed the clinical features of bacterial meningitis associated with strongyloidiasis seen at a single center in subtropical japan, in an area endemic for both strongyloidiasis and htlv-1. we found 33 episodes in 21 patients between 1990 and 2010. the results were remarkable for the high incidence of meningitis due to g ...201323761268
lactic acid bacteria in total mixed ration silage containing soybean curd residue: their isolation, identification and ability to inhibit aerobic deterioration.we investigated the effects of the predominant lactic acid bacteria (lab) on the fermentation characteristics and aerobic stability of total mixed ration (tmr) silage containing soybean curd residue (sc-tmr silage). the sc-tmr materials were ensiled in laboratory silos for 14 or 56 days. lab predominant in sc-tmr silage were identified (exp. 1). lactobacillus fermentum (l. fermentum) and streptococcus bovis (s. bovis) were found in the untreated materials, leuconostoc pseudomesenteroides (l. pse ...201626949952
potentiation by metal ions of the efficacy of the ionophores, monensin and tetronasin, towards four species of ruminal bacteria.concentrations of na(+), k(+) and ca(2+) in the growth medium were varied within limits normally found in vivo to determine how cation concentrations affect the sensitivity of ruminal bacteria to the ionophores, monensin (a na(+)/h(+) and k(+)/h(+) exchanger) and tetronasin (ca(2+)/h(+)). high [na(+)] (172 mm cf. 137 mm in control medium) enhanced the efficacy of monensin towards eubacterium ruminantium 2388, streptococcus bovis c277, lactobacillus casei lb17 and prevotella albensis m384. high [ ...201323210858
isolation and characterization of the microflora of nixtamalized corn masa.corn tortillas are a staple in the diet among the mexican population, and are traditionally produced through a process known as nixtamalization. this traditional process involves steeping whole-kernel corn in an alkaline solution overnight and then grinding the corn into dough (masa), which is then baked. while the masa is held before baking, significant microbial change can occur which leads to fermentation and spoilage. the objective of this research was to characterize and identify the microf ...201323803568
effect of biochanin a on corn grain (zea mays) fermentation by bovine rumen amylolytic bacteria.the objective was to determine the effect of biochanin a (bca), an isoflavone produced by red clover (trifolium pratense l.), on corn fermentation by rumen micro-organisms.201728055130
familial adenomatous polyposis manifesting as lactococcus endocarditis: a case report and review of the association of lactococcus with underlying gastrointestinal disease.a 45-year-old male with a prosthetic aortic valve presented to the hospital with several months of generalized malaise. on admission, he was noted to have anemia of unclear etiology and subsequently became febrile with multiple blood cultures growing lactococcus garvieae. inpatient workup was concerning for infectious endocarditis (ie) secondary to lactococcus. the patient was discharged home with appropriate antimicrobial therapy; however, he was readmitted for persistent, symptomatic anemia an ...201627818810
african fermented dairy products - overview of predominant technologically important microorganisms focusing on african streptococcus infantarius variants and potential future applications for enhanced food safety and security.milk is a major source of nutrients, but can also be a vehicle for zoonotic foodborne diseases, especially when raw milk is consumed. in africa, poor processing and storage conditions contribute to contamination, outgrowth and transmission of pathogens, which lead to spoilage, reduced food safety and security. fermentation helps mitigate the impact of poor handling and storage conditions by enhancing shelf life and food safety. traditionally-fermented sour milk products are culturally accepted a ...201728364623
acquisition through horizontal gene transfer of plasmid psma198 by streptococcus macedonicus aca-dc 198 points towards the dairy origin of the species.streptococcus macedonicus is an intriguing streptococcal species whose most frequent source of isolation is fermented foods similarly to streptococcus thermophilus. however, s. macedonicus is closely related to commensal opportunistic pathogens of the streptococcus bovis/streptococcus equinus complex.201525584532
comparative genomics of the dairy isolate streptococcus macedonicus aca-dc 198 against related members of the streptococcus bovis/streptococcus equinus complex.within the genus streptococcus, only streptococcus thermophilus is used as a starter culture in food fermentations. streptococcus macedonicus though, which belongs to the streptococcus bovis/streptococcus equinus complex (sbsec), is also frequently isolated from fermented foods mainly of dairy origin. members of the sbsec have been implicated in human endocarditis and colon cancer. here we compare the genome sequence of the dairy isolate s. macedonicus aca-dc 198 to the other sbsec genomes in or ...201424713045
activity of oritavancin tested against uncommonly isolated gram-positive pathogens responsible for documented infections in hospitals worldwide.to assess the oritavancin spectrum and activity against 2811 rarer species of coagulase-negative staphylococci (cons), streptococci and other gram-positive species.201424505091
daptomycin activity against uncommonly isolated streptococcal and other gram-positive species groups.a total of 1,356 clinical isolates were tested against daptomycin by broth microdilution methods. daptomycin was active against seven groups of viridans group streptococci (mic50 and mic90 values ranging from ≤0.06 and ≤0.06 μg/ml [streptococcus bovis and streptococcus dysgalactiae] to 0.5 and 1 μg/ml [streptococcus mitis, streptococcus oralis, and streptococcus parasanguinis], respectively), beta-hemolytic streptococci serogroups c, f, and g (mic50 and mic90, ≤0.06 to 0.25 and 0.12 to 0.25 μg/m ...201324080651
potential influence of dairy propionibacteria on the growth and acid metabolism of streptococcus bovis and megasphaera elsdenii.ruminal acidosis is a prevalent disorder among dairy cows and feedlot cattle, which can significantly impair their health and productivity. this study, involving seven different strains of dairy propionibacteria, represents an in vitro investigation of the feasibility of using these organisms as direct-fed microbials to control lactic acid acumulation in the rumen. interactions between the propionibacteria, streptococcus bovis and megasphaera elsdenii were evaluated in terms of effects on lactic ...201727824275
fermentation of alfalfa wet-fractionation liquids to volatile fatty acids by streptococcus bovis and megasphaera elsdenii."green juice", obtained by squeezing fresh alfalfa leaves inoculated with lactic acid bacteria, was fermented at room temperature for 7-21 d to obtain 12-47 g lactic acid l(-1). inoculation of green juice with streptococcus bovis and incubation at 39°c reduced fermentation time to 8-12h. the resulting "brown juice" from either fermentation had a ph of ∼4.5 and a protein precipitate. upon adjustment to ph 5.2-6.8 and inoculation with megasphaera elsdenii, brown juice was fermented within 48 h to ...201323732922
effects of an extract of plant flavonoids (bioflavex) on rumen fermentation and performance in heifers fed high-concentrate diets.to study the effects of an extract of plant flavonoids [bioflavex (fl)] in cattle fed high-concentrate diets, 2 experiments were designed. in the first experiment, the effects of bioflavex on the development of rumen acidosis was evaluated in 8 holstein-friesian crossbreed heifers (451 kg; sem 14.3 kg of bw) using a crossover design. each experimental period lasted 22 d; from d 1 to 20, the animals were fed rye grass, on d 21 the animals were fasted, and on d 22, rumen acidosis was induced by ap ...201222829622
short communication: comparison of ph, volatile fatty acids, and microbiome of rumen samples from preweaned calves obtained via cannula or stomach tube.the objective of this study was to compare rumen samples from young dairy calves obtained via a stomach tube (st) or a ruminal cannula (rc). five male holstein calves (46±4.0 kg of body weight and 11±4.9 d of age) were ruminally cannulated at 15 d of age. calves received 4 l/d of a commercial milk replacer (25% crude protein and 19.2% fat) at 12.5% dry matter, and were provided concentrate and chopped oats hay ad libitum throughout the study (56 d). in total, 29 paired rumen samples were obtaine ...201323706486
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