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current features of infective endocarditis in elderly patients: results of the international collaboration on endocarditis prospective cohort study.elderly patients are emerging as a population at high risk for infective endocarditis (ie). however, adequately sized prospective studies on the features of ie in elderly patients are lacking.200818955638
new strategy for enhancement of microbial viability in simulated gastric conditions based on display of starch-binding domain on cell surface.the c-terminal region of the peptidoglycan hydrolase (cph) of lactococcus lactis il1403 fused to the linker region and the starch-binding domain (sbd) of the *-amylase of streptococcus bovis 148 was produced intracellularly in escherichia coli. the fusion protein (cph-sbd) was able to bind to the cell surface of lactobacillus casei nrrl b-441 and to corn starch. therefore, adhesion of cells to corn starch was mediated by the fusion protein. at a cell density of 10(9) cfu/ml and a starch concentr ...200818558341
molecular properties and transcriptional control of the phosphofructokinase and pyruvate kinase genes in a ruminal bacterium, streptococcus bovis.molecular properties of pyruvate kinase (pyk) and phosphofructokinase (pfk) in streptococcus bovis and transcriptional control of the two enzymes were examined. sequence analysis indicated that the pyk gene (pyk) clusters with the pfk gene (pfk) and several other genes. it was demonstrated that the pyk and pfk are cotranscribed and their transcription appeared to be regulated at the transcriptional level in response to the sugars supplied. the intracellular pyk-mrna level was lower in a cataboli ...200818565772
use of primers based on the heat shock protein genes hsp70, hsp40, and hsp10, for the detection of bovine mastitis pathogens streptococcus agalactiae, streptococcus uberis and streptococcus bovis.streptococcus agalactiae, streptococcus uberis, and streptococcus bovis are three of the major pathogens which cause mastitis in dairy herds. since conventional methods for the detection of these mastitis pathogens are laborious and time-consuming, rapid methods are needed. with an attempt to know if heat shock protein (hsp) genes other than hsp60 gene, could be used for pcr primer designing, in this study, we tried to design pcr primers based on the heat shock protein genes hsp70, hsp40, and hs ...200818602244
comprehensive study of strains previously designated streptococcus bovis consecutively isolated from human blood cultures and emended description of streptococcus gallolyticus and streptococcus infantarius subsp. coli.modern taxonomy has delineated streptococcus gallolyticus subsp. gallolyticus, s. gallolyticus subsp. pasteurianus, streptococcus infantarius subsp. coli, and s. infantarius subsp. infantarius within the heterogenous group of previously designated clinical streptococcus bovis bacteria. in the present study, 58 consecutive blood culture isolates initially designated s. bovis were further characterized by applying phenotypic and molecular genetic methods, and possible disease associations were inv ...200818614655
a traditional sudanese fermented camel's milk product, gariss, as a habitat of streptococcus infantarius subsp. infantarius.samples of the traditional sudanese fermented camel's milk product gariss representing 9 different regions in sudan were microbiologically characterized using an integrated approach including phenotypic and genotypic methods. lactic acid bacteria [log(cfu/g)=7.76-8.66] and yeasts [log(cfu/g)=6.05-7.79] were detected in high numbers. a total of 180 lab isolates were identified of which 123 isolates were found to belong to the streptococcus bovis group. thirteen representative isolates were charac ...200818774196
infectious agents and colorectal cancer: a review of helicobacter pylori, streptococcus bovis, jc virus, and human papillomavirus.based on the high volume of bacteria and viruses that the intestine is exposed to and the importance of infectious agents in some gastrointestinal and anogenital cancers, it is not surprising the many studies have evaluated the association between colorectal cancer and infectious agents. this review highlights investigations of four agents in relation to colorectal cancer. helicobacter pylori, streptococcus bovis, jc virus, and human papillomavirus have all been evaluated as possible etiologic a ...200818990738
identification of streptococcus gallolyticus subsp. macedonicus as the etiological agent in a case of culture-negative multivalve infective endocarditis by 16s rdna pcr analysis of resected valvular tissue.today, pcr using broad-range primers is being used increasingly to detect pathogens from resected heart valves. herein is described the first case of multivalve infective endocarditis where 16s rdna pcr was used to detect a single pathogen from two affected valves in a 61-year-old man. triple heart valve replacement was required despite six weeks of appropriate antimicrobial therapy. the organism was confirmed as streptococcus gallolyticus subsp. macedonicus, a member of the 's. equinus/s. bovis ...200818980096
adherence characteristics of endocarditis-derived streptococcus gallolyticus ssp. gallolyticus (streptococcus bovis biotype i) isolates to host extracellular matrix proteins.members of the streptococcus bovis group are frequent colonizers of the intestinal tract, which can also cause endocarditis. however, their ability to adhere to and colonize host tissues and the factors associated with pathogenicity are largely unknown. here, we assessed 17 endocarditis-derived human isolates [identified here as 15 streptococcus gallolyticus ssp. gallolyticus (s. bovis biotype i), one s. gallolyticus ssp. pasteurianus (biotype ii/2) and one streptococcus infantarius ssp. coli (b ...200819054100
lactobacillus casei secreting alpha-msh induces the therapeutic effect on dss-induced acute colitis in balb/c mice.the neuropeptide alpha-melanocyte-stimulating hormone (alpha- msh) has anti-inflammatory property by downregulating the expressions of proinflammatory cytokines. because alpha-msh elicits the anti-inflammatory effect in various inflammatory disease models, we examined the therapeutic effect of oral administration of recombinant lactobacillus casei, which secretes alpha-msh (l. casei-alpha-msh), on dextran sulfate sodium (dss)-induced colitis in balb/c mice. thus, we constructed the alpha-msh-sec ...200819131702
the ability of non-bacteriocin producing streptococcus bovis strains to bind and transfer bovicin hc5 to other sensitive bacteria.streptococcus bovis hc5 produces a broad spectrum lantibiotic (bovicin hc5), but s. bovis jb1 does not have antimicrobial activity. preliminary experiments revealed an anomaly. when s. bovis jb1 cells were washed in stationary phase s. bovis hc5 cell-free culture supernatant, the s. bovis jb1 cells were subsequently able to inhibit hyper-ammonia producing ruminal bacteria (clostridium sticklandii, clostridium aminophilum and peptostreptococcus anaerobius). other non-bacteriocin producing s. bovi ...200919171197
association between streptococcus bovis and colon cancer. 200919189926
bovicin hc5 inhibits wasteful amino acid degradation by mixed ruminal bacteria in vitro.streptococcus bovis hc5 produces a broad spectrum lantibiotic (bovicin hc5) that inhibits pure cultures of hyper ammonia-producing bacteria (hab). experiments were preformed to see if: (1) s. bovis hc5 cells could inhibit the deamination of amino acids by mixed ruminal bacteria taken directly from a cow, (2) semi-purified bovicin was as effective as s. bovis hc5 cells, and 3) semi-purified and the feed additive monensin were affecting the same types of ammonia-producing ruminal bacteria. because ...200919191869
characteristics of the bovicin hj50 gene cluster in streptococcus bovis hj50.bovicin hj50 is a new lantibiotic containing a disulfide bridge produced by streptococcus bovis hj50; its encoding gene bova was reported in our previous publication. to identify other genes involved in bovicin hj50 production, dna fragments flanking bova were cloned and sequenced. the bovicin hj50 biosynthesis gene locus was encoded by a 9.9 kb region of chromosomal dna and consisted of at least nine genes in the following order: bova, -m, -t, -e, -f, orf1, orf2, bovk and bovr. a thiol-disulfid ...200919202107
physiological changes in rumen fermentation during acidosis induction and its control using a multivalent polyclonal antibody preparation in heifers.physiological changes in rumen fermentation during acidosis induction and its control using a multivalent polyclonal antibody preparation (pap) were studied in a completely randomized experiment using 12 crossbred heifers (452 +/- 20 kg of bw). treatments were control (ctr) or pap. the acidosis induction protocol consisted of 3 periods: 3 mo of 100% fescue hay fed for ad libitum intake, 10 d (from d 1 to 10 of the experiment) of adaptation to the treatment (100% forage feeding + 10 ml/d of pap t ...200919213715
antimicrobial activity of five herbal extracts against multi drug resistant (mdr) strains of bacteria and fungus of clinical origin.antimicrobial activities of the crude ethanolic extracts of five plants were screened against multidrug resistant (mdr) strains of escherichia coli, klebsiella pneumoniae and candida albicans. atcc strains of streptococcus mutans, staphylococcus aureus, enterococcus faecalis, streptococcus bovis, pseudimonas aeruginosa, salmonella typhimurium, escherichia coli, klebsiella pneumoniae and candida albicans were also tested. the strains that showed resistance against the maximum number of antibiotic ...200919214149
streptococcus bovis causing perforating corneal ulcer.the purpose of this study was to report the first case of streptococcus bovis causing a perforating corneal ulcer in a healthy adult.200919092424
ecology and pathogenicity of gastrointestinal streptococcus bovis.streptococcus bovis is an indigenous resident in the gastrointestinal tracts of both humans and animals. s. bovis is one of the major causes of bacterial endocarditis and has been implicated in the incidence of human colon cancer, possibly due to chronic inflammatory response at the site of intestinal colonization. certain feeding regimens in ruminants can lead to overgrowth of s. bovis in the rumen, resulting in the over-production of lactate and capsular polysaccharide causing acute ruminal ac ...200919100852
direct production of cadaverine from soluble starch using corynebacterium glutamicum coexpressing alpha-amylase and lysine decarboxylase.here, we demonstrated the one-step production of cadaverine from starch using a corynebacterium glutamicum strain coexpressing streptococcus bovis 148 alpha-amylase (amya) and escherichia coli k-12 lysine decarboxylase (cada). we constructed the e. coli-c. glutamicum shuttle vector, which produces cada under the control of the high constitutive expression (hce) promoter, and transformed this vector into c. glutamicum css secreting amya. the engineered c. glutamicum expressed both cada and amya, ...200918989633
efficient production of optically pure d-lactic acid from raw corn starch by using a genetically modified l-lactate dehydrogenase gene-deficient and alpha-amylase-secreting lactobacillus plantarum strain.in order to achieve direct and efficient fermentation of optically pure d-lactic acid from raw corn starch, we constructed l-lactate dehydrogenase gene (ldhl1)-deficient lactobacillus plantarum and introduced a plasmid encoding streptococcus bovis 148 alpha-amylase (amya). the resulting strain produced only d-lactic acid from glucose and successfully expressed amya. with the aid of secreting amya, direct d-lactic acid fermentation from raw corn starch was accomplished. after 48 h of fermentation ...200919011066
properties and role of glyceraldehyde-3-phosphate dehydrogenase in the control of fermentation pattern and growth in a ruminal bacterium, streptococcus bovis.to clarify the control of glycolysis and the fermentation pattern in streptococcus bovis, the molecular and enzymatic properties of nad(+)-specific glyceraldehyde-3-phosphate dehydrogenase (gapdh) were examined. the gapdh gene (gapa) was found to cluster with several others, including those that encode phosphoglycerate kinase and translation elongation factor g, however, gapa was transcribed in a monocistronic fashion. since biochemical properties, such as optimal ph and affinity for glyceraldeh ...200919034572
screening of surface properties and antagonistic substances production by lactic acid bacteria isolated from the mammary gland of healthy and mastitic cows.bovine mastitis (bm) is a costly disease in dairy cattle production. the prevention and treatment of mastitis is performed by applying antimicrobial products that negatively affect milk quality. in the last years, the use of probiotic microorganisms to prevent infections in humans and animals has being aggressively studied. samples from teat canal and milk (foremilk and stripping) were taken from healthy and mastitic mammary quarters. a screening of the surface properties and antagonistic substa ...200919041199
biotransformation of 2,4,6-trinitrotoluene by pure culture ruminal bacteria.twenty-one ruminal bacteria species were tested for their ability to degrade 2,4,6-trinitrotoluene (tnt) within 24 h. butyrivibrio fibrisolvens, fibrobacter succinogenes, lactobacillus vitulinus, selenomonas ruminantium, streptococcus caprinus, and succinivibrio dextrinosolvens were able to completely degrade 100 mg/l tnt, with <5% of the original tnt recovered as diaminonitrotoluene metabolites. eubacterium ruminantium, lactobacillus ruminis, ruminobacter amylophilus, streptococcus bovis, and w ...200918839246
streptococcus bovis bacteraemia: identification within organism complex and association with endocarditis and colonic malignancy.to identify streptococcus bovis bacteraemia episodes and their clinical associations, including differences in associations by s. bovis biotypes (or species).200918972318
neonatal bacterial meningitis caused by streptococcus gallolyticus subsp. pasteurianus.this report describes a case of neonatal bacteraemia and meningitis due to streptococcus gallolyticus subsp. pasteurianus. based on the identification kit results, this species may have been reported as streptococcus bovis or s. bovis biotype ii. the accurate identification of this organism is mandatory for evaluating the aetiology of neonatal meningitis.200919528187
pathogen detection in milk samples by ligation detection reaction-mediated universal array method.this paper describes a new dna chip, based on the use of a ligation detection reaction coupled to a universal array, developed to detect and analyze, directly from milk samples, microbial pathogens known to cause bovine, ovine, and caprine mastitis or to be responsible for foodborne intoxication or infection, or both. probes were designed for the identification of 15 different bacterial groups: staphylococcus aureus, streptococcus agalactiae, nonaureus staphylococci, streptococcus bovis, strepto ...200919528580
effects of dietary changes and yeast culture (saccharomyces cerevisiae) on rumen microbial fermentation of holstein heifers.the effects of a dietary challenge to induce digestive upsets and supplementation with yeast culture on rumen microbial fermentation were studied using 12 holstein heifers (277 +/- 28 kg of bw) fitted with a ruminal cannula, in a crossover design with 2 periods of 5 wk. in each period, after 3 wk of adaptation to a 100% forage diet, the dietary challenge consisted of increasing the amount of grain at a rate of 2.5 kg/d (as-fed basis) over a period of 4 d, until a 10:90 forage:concentrate diet wa ...200919542509
the effect of carbon and nitrogen sources on bovicin hc5 production by streptococcus bovis hc5.to investigate the effect of media composition and agroindustrial residues on bovicin hc5 production by streptococcus bovis hc5.200919320950
association of streptococcus pluranimalium with valvular endocarditis and septicaemia in adult broiler parents.the genus streptococcus consists of more than 60 species, but only streptococcus equi subspecies zooepidemicus, streptococcus gallolyticus ssp. gallolyticus, streptococcus gallinaceus, streptococcus dysgalactiae, streptococcus mutans and streptococcus suis have been isolated from poultry. during investigations of the aetiology of increased mortality in broiler parent stock at the end of production, pure cultures of streptococcal-like organisms that could not be classified among these six species ...200919322715
a pyogenic discitis at c3-c4 with associated ventral epidural abscess involving c1-c4 after intradiscal oxygen-ozone chemonucleolysis: a case report.a case report and clinical discussion.200919365239
the clinical importance of microbiological findings in the diagnosis and management of bloodstream infections.bloodstream infections are associated with high morbidity and mortality. accurate identification of blood isolates to the species level and identification of the source of infection and/or the portal of entry are crucial for optimal management of these infections. these investigations-in addition to clinical findings and laboratory and imaging studies-are central to informing and directing efficient and effective diagnostic examinations and to choosing the optimal antimicrobial regimen. four cas ...200919374579
development of novel cell surface display in corynebacterium glutamicum using porin.we have developed a novel cell surface display in corynebacterium glutamicum using porin proteins as anchor proteins. porins are localized at c. glutamicum mycolic acid layer and exist as a hexamer. we used alpha-amylase from streptococcus bovis 148 (amya) as a model protein to be displayed on the c. glutamicum cell surface. amya was fused to the c terminus of the porins porb, porc, or porh. expression vectors using fused proteins under the control of the cspb promoter were constructed and intro ...200919430772
a collagen-binding adhesin, acb, and ten other putative mscramm and pilus family proteins of streptococcus gallolyticus subsp. gallolyticus (streptococcus bovis group, biotype i).members of the streptococcus bovis group are important causes of endocarditis. however, factors associated with their pathogenicity, such as adhesins, remain uncharacterized. we recently demonstrated that endocarditis-derived streptococcus gallolyticus subsp. gallolyticus isolates frequently adhere to extracellular matrix (ecm) proteins. here, we generated a draft genome sequence of an ecm protein-adherent s. gallolyticus subsp. gallolyticus strain and found, by genome-wide analyses, 11 predicte ...200919717590
characteristics of xanthan gum-based biodegradable superporous hydrogel.a novel biopolymer-based superporous hydrogel (sph) was synthesized through chemical crosslinking by graft copolymerization of 2-hydroxyethyl methacrylate (hema) and acrylic acid (aa) on to xanthan gum (xg) via redox initiator system of ammonium persulfate (aps) and n, n, n', n'-tetramethylethylenediamine (tmed), in the presence of n, n'-methylenebisacrylamide (mba) crosslinking agent, sodium bicarbonate foaming agent, a triblock copolymer of polyoxyethylene/polyoxypropylene/polyoxyethylene as a ...200919643130
[septic shock caused by streptococcus bovis in a haemodialysis patient]. 200919668325
aorto-pulmonary fistula as a late complication of multiple valve replacement after streptococcus bovis endocarditis in a patient with occult colon carcinoma.we report a case of aorto-pulmonary fistula following multiple valve surgery in a patient with colon carcinoma who had had recurrent episodes of streptococcus bovis endocarditis. diagnosis was made by transesophageal echocardiography and aortography. patient underwent emergency percutaneous implantation of an aortic endoprosthesis, but he continued to deteriorate and the fatal outcome could not be changed. this case underscores the importance of examination of gastrointestinal tract in patients ...200919253356
[novel clinical and epidemiologic trends in elderly infective endocarditis].the age of peak incidence of infective endocarditis (ie) has shifted from 45 years in the 50's to 70 years at the present time. elderly persons carry a five times higher risk of ie than the general adult population. factors accounting for this phenomenon include increased use of invasive procedures and implanted medical devices as well as a higher prevalence of degenerative valve disease. in the elderly, ie onset may be insidious, often masked by one or more underlying conditions such as cancer, ...200919708302
relative penicillin resistance in streptococcus bovis. a case of neonatal meningitis. 200919712186
streptococcus gallolyticus subspecies gallolyticus infection in ducklings. 200919734564
surface-exposed histone-like protein a modulates adherence of streptococcus gallolyticus to colon adenocarcinoma cells.streptococcus gallolyticus (formerly known as streptococcus bovis biotype i) is a low-grade opportunistic pathogen which is considered to be associated with colon cancer. it is thought that colon polyps or tumors are the main portal of entry for this bacterium and that heparan sulfate proteoglycans (hspgs) at the colon tumor cell surface are involved in bacterial adherence during the first stages of infection. in this study, we have shown that the histone-like protein a (hlpa) of s. gallolyticus ...200919752027
rumen microbiome composition determined using two nutritional models of subacute ruminal acidosis.subacute ruminal acidosis (sara) is a metabolic disease in dairy cattle that occurs during early and mid-lactation and has traditionally been characterized by low rumen ph, but lactic acid does not accumulate as in acute lactic acid acidosis. it is hypothesized that factors such as increased gut permeability, bacterial lipopolysaccharides, and inflammatory responses may have a role in the etiology of sara. however, little is known about the nature of the rumen microbiome during sara. in this stu ...200919783747
malpractice issues in radiology: res ipsa loquitur. 200919933635
uterine fibroid embolization. 200919967821
changes in faecal bacteria associated with concentrate and forage-only diets fed to horses in training.diets rich in readily fermentable carbohydrates, fed traditionally to meet the increased energy requirements of the performance horse, are associated with a number of gastrointestinal disorders that involve disturbances in the intestinal microbiota, however, these changes are poorly understood.200920383990
unicuspid aortic valve in a patient with streptococcus bovis endocarditis. 200920099730
characterization of bacteriocin produced by streptococcus bovis j2 40-2 isolated from traditional fermented milk 'dahi'.a bacteriocin-producing strain streptococcus bovis j2 40-2 was isolated from traditional fermented milk 'dahi' in bangladesh. despite its narrow antimicrobial spectrum, it showed strong antimicrobial activity against extremely challenging and problematic organisms in foods, such as listeria monocytogenes. bacteriocin was sensitive to several proteolytic enzymes and showed antimicrobial activity over a wide ph range of 2.0-10.0. it was stable when heated to 110 degrees c for 20 min, but lost 25% ...200920163471
identification of lactic acid bacteria associated with the production of plaa-som, a traditional fermented fish product of thailand.plaa-som is a thai fermented fish product for which whole fish or fish fillets are fermented with either cooked rice or steamed sticky rice, salt, and garlic. a total of 762 lactic acid bacteria (lab) were isolated during plaa-som fermentation by culture on caco(3)-mrs agar plates. they were screened and grouped by amplified ribosomal dna restriction analysis (ardra), giving six groups that were identified by ribosomal dna sequencing as lactococcus garvieae, streptococcus bovis, weissella cibari ...201020167386
streptococcus gallolyticus subspecies pasteurianus (biotype ii/2), a newly reported cause of adult meningitis.we report the first case of adult meningitis confirmed to be due to streptococcus gallolyticus subsp. pasteurianus. phenotypically reported as streptococcus bovis biotype ii/2, 16s rrna sequencing revealed s. gallolyticus subsp. pasteurianus. because of taxonomic uncertainties, s. gallolyticus subsp. pasteurianus may be an underrecognized agent of systemic infections.201020357211
effect of lactobacillus casei on streptococcus bovis in faecal flora.bacteraemia caused by streptococcus bovis is often associated with colorectal tumours. also, experimental studies have been proposed that s bovis acts as a promoter of tumours. we report the case of a man with colon adenoma who had a high proportion of s bovis in his faecal flora. he was treated with a lactobacillus casei preparation (blp) and the effect on the faecal flora was examined. l casei reduced the proportion of s bovis (from 43% down to 9%), and the effect continued after the administr ...201022242064
genome sequence of streptococcus gallolyticus: insights into its adaptation to the bovine rumen and its ability to cause endocarditis.streptococcus gallolyticus (formerly known as streptococcus bovis biotype i) is an increasing cause of endocarditis among streptococci and frequently associated with colon cancer. s. gallolyticus is part of the rumen flora but also a cause of disease in ruminants as well as in birds. here we report the complete nucleotide sequence of strain ucn34, responsible for endocarditis in a patient also suffering from colon cancer. analysis of the 2,239 proteins encoded by its 2,350-kb-long genome reveale ...201020139183
age-dependent profile of left-sided infective endocarditis: a 3-center experience.the influence of age on the main epidemiological, clinical, echocardiographic, microbiological, and prognostic features of patients with infective endocarditis remains unknown. we present the series with the largest numbers and range of ages of subjects to date that analyzes the influence of age on the main characteristics of patients with isolated left-sided infective endocarditis. furthermore, this series is the first one in which patients have been distributed according to age quartile.201020142448
special considerations apply when streptococcus bovis is the culprit pathogen. 201020159404
is it antiphospholipid syndrome?the diagnosis of bacterial endocarditis remains a challenge, as nearly half of cases develop in the absence of preexistent heart disease and known risk factors. not infrequently, a blunted clinical course at onset can lead to erroneous diagnoses. we present the case of a 47-year-old previously healthy man in which a presumptive diagnosis of antiphospholipid syndrome was made based on the absence of echocardiographically detected heart involvement, a negative blood culture, normal c-reactive prot ...201021318137
prevalence of colorectal cancer associated with streptococcus bovis among inflammatory bowel and chronic gastrointestinal tract disease patients.colorectal cancer (crc) is the second most common cause of cancer mortality among men and women worldwide; the risk of its occurrence has been shown to be increased by chronic bacterial infections. a case control study was therefore carried out at hospital universiti sains malaysia (husm) to determine the incidence of colorectal cancer associated with s. bovis infection. a total of 166 stool specimens were collected from diseased patients and healthy individuals and s. bovis isolates were identi ...201021338230
streptococcus bovis bacteraemia revisited: clinical and microbiological correlates in a contemporary series of 59 patients.to characterise the clinical features, associations and outcome in a contemporary series of patients with streptococcus bovis bacteraemia (sbb).201020659498
streptococcus bovis infection of total hip arthroplasty in association with carcinoma of colon.streptococcus bovis is normally found in the gastrointestinal tract of the human population. the association between streptococcus bovis and bowel neoplasia has been frequently reported. this report presents two cases of total hip arthroplasty with streptococcus bovis infection at 18 and 11 years after arthroplasty. the diagnosis was made with the help of clinical findings, standard radiographs, and laboratory tests (complete blood count, c-reactive protein, sedimentation rate, and the hip aspir ...201020727310
spontaneous bacterial peritonitis caused by streptococcus bovis: case report and review of the literature.spontaneous bacterial peritonitis (sbp) is a frequent and severe complication that occurs in patient with cirrhosis and ascites. it occurs in 10% to 30% of patients admitted to hospital. the organisms that cause sbp are predominantly enteric. escherichia coli is the most frequent recovered pathogen, and gram-positive bacteria, mainly staphylococcus spp., are being considered an emerging causative agent of sbp. streptococcus bovis that may be found as part of the commensal bowel flora in about 10 ...201020835516
rumen microbial population dynamics during adaptation to a high-grain diet.high-grain adaptation programs are widely used with feedlot cattle to balance enhanced growth performance against the risk of acidosis. this adaptation to a high-grain diet from a high-forage diet is known to change the rumen microbial population structure and help establish a stable microbial population within the rumen. therefore, to evaluate bacterial population dynamics during adaptation to a high-grain diet, 4 ruminally cannulated beef steers were adapted to a high-grain diet using a step-u ...201020851965
association of symptoms with gastrointestinal microbiota in irritable bowel syndrome.to investigate the correlations between self-reported symptoms of irritable bowel syndrome (ibs) and the gastrointestinal (gi) microbiota composition.201020857523
infective endocarditis caused by streptococcus bovis complicated by a superior mesenteric artery mycotic aneurysm and systemic septic emboli in a patient with colon diverticulitis.to date, streptococcus bovis endocarditis complicated by a superior mesenteric artery mycotic aneurysm and systemic septic emboli in a patient with colon diverticulitis has never been reported. a 46-year-old man, with a history of colon diverticulitis identified by diagnostic colon fiberscope and with hypertension, presented with a 14-day history of intermittent fever. an echocardiogram revealed severe mitral regurgitation with two large floating vegetations attached to both mitral leaflets. a b ...201020932486
infectious endocarditis due to streptococcus bovis in a patient with colon carcinoma.we report the case of a 66 year-old female patient with infectious endocarditis due to streptococcus bovis and adenocarcinoma of the colon that developed acute aortic insufficiency. she was submitted to aortic valve replacement surgery and later to tumor resection (right hemicolectomy). it is important to emphasize the need for complementing the study of the colon, even in asymptomatic individuals, when infectious endocarditis due to s. bovis is diagnosed.201020944897
microbial and viral pathogens in colorectal cancer.the heterogenetic and sporadic nature of colorectal cancer has led to many epidemiological associations with causes of this disease. as our understanding of the underlying molecular processes in colorectal-cancer develops, the concept of microbial-epithelial interactions as an oncogenic trigger might provide a plausible hypothesis for the pathogenesis of colorectal cancer. by contrast with other cancers of the gastrointestinal tract (gastric carcinoma, mucosa-associated lymphoid-tissue lymphoma) ...201021067973
autoregulation of lantibiotic bovicin hj50 biosynthesis by the bovk-bovr two-component signal transduction system in streptococcus bovis hj50.streptococcus bovis hj50 produces a lacticin 481-like 33-amino-acid-residue lantibiotic, designated bovicin hj50. bovk-bovr in the bovicin hj50 biosynthetic gene cluster is predicted to be a two-component signal transduction system involved in sensing signals and regulating gene expression. disruption of bovk or bovr resulted in the abrogation of bovicin hj50 production, suggesting both genes play important roles in bovicin hj50 biosynthesis. addition of exogenous bovicin hj50 peptide to culture ...201021075878
gene cluster for biosynthesis of thermophilin 1277--a lantibiotic produced by streptococcus thermophilus sbt1277, and heterologous expression of tepi, a novel immunity peptide.to identify genes cluster for thermophilin 1277 produced by streptococcus thermophilus sbt1277.201021182576
acute cholecystitis and bacteraemia due to streptococcus bovis biotype ii. 201021195508
dark hypopyon in streptococcus bovis endogenous endophthalmitis: clinicopathologic correlations.the aim of this report is to present a previously unreported causative organism associated with brown-pigmented hypopyon in a patient with endophthalmitis.201021475652
rumen chemical and bacterial changes during stepwise adaptation to a high-concentrate diet in goats.the correlation between rumen chemical and bacterial changes was investigated during a four periodical stepwise adaptation to a high-concentrate diet (concentrate level at 0%, 30%, 50% and 70% for diet i to iv, respectively) in goats. the results showed that ruminal ph decreased from 6.7 to 5.5 after switching from diet i to ii, and was maintained at about 5.5 on diet iii. denaturing gradient gel electrophoresis results showed that the rumen bacterial community was relatively stable during the i ...201022443874
streptococcus sanguis endocarditis associated with colonic carcinoma.infective endocarditis caused by streptococcus bovis is known to be associated with colorectal malignancy. other less common streptococci, specifically streptococcus sanguis, can be similarly associated with gastrointestinal carcinoma. we present a case of disseminated colorectal carcinoma occurring after a confirmed s sanguis endocarditis, that required mitral valve surgery. there may be a need for gastrointestinal surveillance in patients presenting with bacteraemia caused by less common strep ...201022347887
[aortic endocarditis and spondylodiscitis due to streptococcus bovis in a patient in his eighties with colon cancer]. 201020399536
the incidence of bacteremia after argon plasma coagulation in patients with chronic radiation proctocolitis.abstract aim: argon plasma coagulation (apc) is considered a safe treatment for hemorrhagic chronic radiation proctocolitis (crpc), but bacteremia is a rare complication. the study aimed to evaluate its frequency after apc for crpc. method: a prospective study of 21 patients who underwent apc (30 procedures) for crpc was carried out. blood cultures (bactec(r)) were obtained: before and 30 minutes after the procedure (60 samples total). patients were monitored for 48 hours after the procedure to ...201020402735
streptococcus bovis meningitis and hemorrhoids.we report a case of streptococcus bovis (streptococcus gallolyticus subsp. pasteurianus) meningitis, a rare cause of central nervous system (cns) infection in an adult, and comment on the importance of investigation of the lower gastrointestinal tract to identify a portal of entry in cases of systemic streptococcus bovis infection.201020421434
a case of streptococcus gallolyticus subsp. gallolyticus infective endocarditis with colon cancer: identification by 16s ribosomal dna sequencing.although the association between streptococcus bovis endocarditis and colon carcinoma is well known, very few cases of s. bovis infection associated with underlying malignancies have been reported in korea. the s. bovis group has been recently reclassified and renamed as streptococcus gallolyticus and streptococcus infantarius subspecies under a new nomenclature system. we report a case of infective endocarditis with colon cancer caused by s. gallolyticus subsp. gallolyticus (previously named s. ...201020445334
bacterial meningitis in cirrhotic patients: case series and description of the prognostic role of acute renal failure.to analyze the mortality risk factors in cirrhotic patients with bacterial meningitis (bm).201020453662
effects of hops (humulus lupulus l.) extract on volatile fatty acid production by rumen bacteria.to determine the effects of hops extract on in vitro volatile fatty acid (vfa) production by bovine rumen micro-organisms.201020456526
involvement of two-component signal transduction system, comde, in the regulation of growth and genetic transformation, in the ruminal bacterium streptococcus bovis.in ruminants, streptococcus bovis is considered to be associated with acute rumen acidosis. to elucidate the regulatory mechanisms of s. bovis growth, we investigated the function of the two components of the peptide pheromone-signaling system, comd and come, which are encoded by comd and come, respectively, via the competence-stimulating peptide comc, which is encoded by comc. deletion of entire comc and two-thirds of comd resulted in decreased growth rate, which may be related to the change in ...201020478389
molecular characterization and significance of phosphoenolpyruvate carboxykinase in a ruminal bacterium, streptococcus bovis.to gain knowledge about the significance of phosphoenolpyruvate (pep) carboxykinase (pck) in streptococcus bovis, the sequence of the gene encoding pck (pck) was determined. transcriptional analysis indicated that the pck is transcribed in a monocistronic fashion. the level of pck-mrna was higher when cells were grown on lactose than on glucose, suggesting that pck synthesis increases when the growth rate is low. the pck-mrna level was higher in a mutant lacking ccpa, which encodes the catabolit ...201020513959
activity of solvent extracts of prosopis spicigera, zingiber officinale and trachyspermum ammi against multidrug resistant bacterial and fungal strains.the emerging trends of multidrug resistance among several groups of microorganisms against different classes of antibiotics led different researchers to develop efficient drugs from plant sources to counter multidrug resistant strains. this study investigated different solvent extracts of prosopis spicigera (p. spicigera), zingiber officinale, and trachyspermum ammi (t. ammi) to determine their efficacy against multidrug resistant microbes.201020539061
increased exposure to bacterial antigen rpl7/l12 in early stage colorectal cancer patients.cancer 2010. (c) 2010 american cancer society. : intestinal bacteria have long been implicated in colorectal cancer pathology, and many reports point to a close linkage between streptococcus bovis biotype i (recently renamed streptococcus gallolyticus) infections and tumors of the human colon. this work aims to investigate the humoral immune response to this bacterium during different stages of colorectal cancer.201020564125
high temporal and inter-individual variation detected in the human ileal microbiota.the diversity and temporal stability of the predominant bacteria in the human ileum was studied with the use of ileal effluent samples of seven individuals with brooke ileostomies. the total number of bacteria within the ileal effluent was in the range of 10⁷ -10⁸ bacteria per gram (wet weight). the diversity of the bacteria in the ileal effluent showed marked differences compared with that in faecal samples from age-matched healthy adults. the ileal effluent had a higher relative abundance of s ...201020626454
description of development of rumen ecosystem by pcr assay in milk-fed, weaned and finished lambs in an intensive fattening system.this study examined the reticulo-rumen characteristics of the microbial community and its fermentative characteristics in milk-fed, at weaning and finished lambs in a conventional fattening system. five lambs were assigned to each of three groups: milk-fed lambs slaughtered at 30 days (t30), weaned lambs slaughtered at 45 days (t45) and 'finished lambs' slaughtered at 90 days (t90). at slaughter, rumen size, fermentation parameters (ph, volatile fatty acids and microbial enzyme activity) and pro ...201020050953
effects of acarbose on ruminal fermentation, blood metabolites and microbial profile involved in ruminal acidosis in lactating cows fed a high-carbohydrate ration.the objective was to evaluate the effects of an inhibitor of alpha-amylase and glucosidase (acarbose, pfizer limited, corby, uk) on ruminal fermentation, blood metabolism and microbial profile in dairy cows in a 2x2 cross-over experiment. eight holstein cows fitted with rumen cannulas (milk yield, 24.3+/-2.35 kg/d, body weight, 622+/-54 kg, days in milk, 183+/-67, 5 multiparous and 3 primiparous) were used. treatments were: control (no additive, ctr) and alpha-amylase and glucosidase inhibitor ( ...201020053317
ph dynamics and bacterial community composition in the rumen of lactating dairy cows.the influence of ph dynamics on ruminal bacterial community composition was studied in 8 ruminally cannulated holstein cows fitted with indwelling electrodes that recorded ph at 10-min intervals over a 54-h period. cows were fed a silage-based total mixed ration supplemented with monensin. ruminal samples were collected each day just before feeding and at 3 and 6h after feeding. solid and liquid phases were separated at collection, and extracted dna was subjected to pcr amplification followed by ...201020059926
identification of tetracycline- and erythromycin-resistant gram-positive cocci within the fermenting microflora of an italian dairy food product.microbiological and molecular analysis of antibiotic resistance in gram-positive cocci derived from the italian pdo (protected designation of origin) dairy food product mozzarella di bufala campana.201020092542
streptococcus bovis bacteremia related to colon adenoma in a chronic hemodialysis patient.abstract we report the case of a 54-year-old hemodialysis patient who presented with recurrent fever due to streptococcus bovis bacteremia related to colonic tubulovillous adenoma. following polypectomy and broad-spectrum antibiotic therapy, the patient totally recovered. in this paper, we discussed the relation between s. bovis bacteremia, colonic adenomas, and hemodialysis. awareness of this association is critical for early diagnosis and management.201019758303
novel strategy for yeast construction using delta-integration and cell fusion to efficiently produce ethanol from raw starch.we developed a novel strategy for constructing yeast to improve levels of amylase gene expression and the practical potential of yeast by combining delta-integration and polyploidization through cell fusion. streptococcus bovis alpha-amylase and rhizopus oryzae glucoamylase/alpha-agglutinin fusion protein genes were integrated into haploid yeast strains. diploid strains were constructed from these haploid strains by mating, and then a tetraploid strain was constructed by cell fusion. the alpha-a ...201019707752
[streptococcus bovis endocarditis in the four native valves. a case description]. 201019729229
production of bacteriocins by streptococcus bovis strains from australian ruminants.to examine the prevalence of bacteriocin production in streptococcus bovis isolates from australian ruminants and the feasibility of industrial production of bacteriocin.201019614851
[recurrent infective endocarditis. a case report].we report the case of a 63-year old patient presenting five infective endocarditis (ie) in a 13-years period, with different types of streptococcus. no entrance way had been found until the last relapse occurring on mitro-aortic bioprosthesis. this recurrence leads to the diagnosis of caecal adenocarcinoma by coloscopy, preceded and guided by pet scanner. it seems to be the initial entrance way which had been unrecognized because of its small size and its particular location, that is very diffic ...201018930174
streptococcus bovis endocarditis, a silent sign for colonic tumour.an association between colonic carcinoma and streptococcus bovis endocarditis/bacteraemia was first suggested in 1951. this knowledge has great clinical implications, yet we found scant awareness amongst general surgical trainees. the aim of this article was to review the evidence available in the literature and explore the pathophysiology behind this association.201019226366
dissection of the bridging pattern of bovicin hj50, a lantibiotic containing a characteristic disulfide bridge.bovicin hj50, a lantibiotic produced by streptococcus bovis hj50, is featured by the presence of a disulfide bridge. this study described a simplified in vitro synthetic strategy for producing bovicin hj50 totally based on escherichia coli expression system. in this strategy termed as semi-in vitro biosynthesis (sivb), prepeptide bova and modification enzyme bovm were co-expressed to generate posttranslationally modified bova. then a specific protease bovt150 was employed to remove the leader pe ...201120630724
effects of adding polyclonal antibody preparations on ruminal fermentation patterns and digestibility of cows fed different energy sources.nine ruminally cannulated cows fed different energy sources were used to evaluate an avian-derived polyclonal antibody preparation (pap-mv) against the specific ruminal bacteria streptococcus bovis, fusobacterium necrophorum, clostridium aminophilum, peptostreptococcus anaerobius and clostridium sticklandii and monensin (mon) on ruminal fermentation patterns and in vivo digestibility. the experimental design was three latin squares 3 x 3 distinguished by the main energy source in the diet [dry-g ...201121551346
spontaneous bacterial peritonitis secondary to streptococcus bovis in a patient with chronic chylous ascites.spontaneous bacterial peritonitis (sbp) is a common complication of cirrhosis and ascites, and is predominantly caused by enteric organisms. streptococcus bovis is a rare etiology of sbp that was first reported in 1994. since then, few prior reports of sbp secondary to s. bovis have been observed in patients with underlying cirrhosis or hepatitis. we now present a case of sbp caused by s. bovis in the setting of chronic chylous ascites in a patient with no known liver pathology.201121602688
isolated septic discitis associated with streptococcus bovis bacteremia.streptococcus bovis is a gram-positive coccus that can be found in the intestinal flora of healthy people; it is also associated with colon cancer and infective endocarditis. we report on a 79-year-old male who initially presented with acute-onset lower back pain. streptococcus bovis was detected in repeated blood cultures, and magnetic resonance imaging of the lumbar spine revealed septic discitis of the l2-l3 intervertebral disc. excision and debridement of the intervertebral disc was performe ...201121606726
screening of species-specific lactic acid bacteria for veal calves multi-strain probiotic adjuncts.the selection of promising specific species of lactic acid bacteria with potential probiotic characteristics is of particular interest in producing multi species-specific probiotic adjuncts in veal calves rearing. the aim of the present work was to select and evaluate in vitro the functional activity of lactic acid bacteria, bifidobacterium longum and bacillus coagulans strains isolated from veal calves in order to assess their potential use as multi species-specific probiotics for veal calves. ...201121619939
detection and characterization of pediocin pa-1/ach like bacteriocin producing lactic acid bacteria.fifty-five bacteriocinogenic lactic acid bacteria (lab) isolated from seven different sources. eight isolates were found to produce pediocin pa-1 like bacteriocin as detected by pedb gene pcr and dot-blot hybridization. the culture filtrate (cf) activity of these isolates exhibited strong antilisterial, antibacterial activity against tested food-borne pathogens and lab. the identification and genetic diversity among the selected lab was performed by conventional morphological and molecular tools ...201121656248
streptococcus bovis peritonitis complicating peritoneal dialysis--a review of 10 years' experience.objective: an association of streptococcus bovis bacteremia with carcinoma of colon has been reported, but data regarding peritoneal dialysis (pd) peritonitis caused by s. bovis is scarce. in this study, we examined the clinical characteristics, associations, and outcomes of this diseaseentity. ? methods: the case records of patients with s. bovis pd peritonitis presenting to 2 renal centers between january 2000 and september 2010 were reviewed. clinical features and outcomes were identified and ...201121804137
antimicrobial activity of daptomycin tested against gram-positive strains collected in european hospitals: results from 7 years of resistance surveillance (2003-2009).daptomycin is a cyclic lipopeptide approved by the european medicines agency (emea) for the treatment of complicated skin and soft tissue infections (cssti) and staphylococcus aureus bacteremia and endocarditis. we evaluated the in vitro activity of daptomycin and comparators tested against clinical isolates from european hospitals over a 7-year period (2003-2009). a total of 36,769 consecutive isolates were collected in 34 medical centers located in 13 european countries, turkey and israel. the ...201121803696
re-identification of streptococcus bovis isolates causing bacteraemia according with the new taxonomy criteria: still an issue?all streptococcus bovis blood culture isolates recovered from jan-2003 to jan-2010 (n=52) in our institution were re-identified using new taxonomic criteria based upon their genetic traits. initial identification was performed by the semiautomatic wider system (fco. soria-melguizo, spain) and the api 20 strep (biomérieux, france). all isolates were re-identified by pcr amplification and sequencing of both 16s rdna and soda genes and by mass spectrometry using maldi-tof ms (bruker, germany). resu ...201121752968
[descriptive analysis of diseases associated with streptococcus bovis bacteremia. suggestion of a first protocol of study.]background and objective: it is well-known the relationship between streptococcus bovis (s. bovis) bacteremia and colon cancer, liver cirrhosis and others neoplasms. however, a study protocol to rule out these underlying diseases has not been carried out yet. our objective was to describe s. bovis bacteremia and associated diseases. patients and method: multicenter, retrospective cohort study. s. bovis bacteremias episodes between 2001 and 2009 were included. mean variables: colon neoplasm, non- ...201121719046
two weeks of postsurgical therapy may be enough for high-risk cases of endocarditis caused by streptococcus viridans or streptococcus bovis.clin microbiol infect abstract: the duration of antimicrobial therapy after surgery for infective endocarditis (ie) is controversial. a short course of postsurgical therapy is currently accepted only for patients with negative valve culture. we performed a retrospective (1994-2008) analysis of patients who underwent surgery for ie in our hospital and had a high risk of complications ( one of more of the following: <2-áweeks of antibiotic treatment before surgery; embolism; perivalvular extension ...201121733030
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