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antiprotozoal effect of saponins in the rumen can be enhanced by chemical modifications in their structure.the antiprotozoal effect of saponins is transitory, as when saponins are deglycosylated to the sapogenin by rumen microorganisms they become inactive. we postulated that the substitution of the sugar moiety of the saponin with small polar residues would produce sapogen-like analogs which might be resistant to degradation in the rumen as they would not be enzymatically cleaved, allowing the antiprotozoal effect to persist over time. in this study, we used an acute assay based on the ability of pr ...201728382023
african fermented dairy products - overview of predominant technologically important microorganisms focusing on african streptococcus infantarius variants and potential future applications for enhanced food safety and security.milk is a major source of nutrients, but can also be a vehicle for zoonotic foodborne diseases, especially when raw milk is consumed. in africa, poor processing and storage conditions contribute to contamination, outgrowth and transmission of pathogens, which lead to spoilage, reduced food safety and security. fermentation helps mitigate the impact of poor handling and storage conditions by enhancing shelf life and food safety. traditionally-fermented sour milk products are culturally accepted a ...201728364623
isolation of streptococci from a fatal case of myocarditis in a captive brown bear (ursus arctos).a 10-yr-old, male brown bear ( ursus arctos ) from bursa zoo in turkey died without any apparent signs. severe purulent pericarditis and myocarditis with mild ascites, lung edema, and moderate liver congestion were observed during necropsy. microscopically, there were severe neutrophilic infiltrations in the myocardium and thoracic lymph nodes. a member of the streptococcus bovis - streptococcus equinus complex (sbsec) was isolated and identified phenotypically.201728363068
effect of sugarcane fiber digestibility, conservation method and concentrate level on the ruminal ecosystem of beef cattle.the aim of this study was to investigate the effects of sugarcane neutral detergent fiber digestibility (ndfd), conservation method, and concentrate level on the ruminal microbial population of steers. eight ruminal-cannulated nellore steers were distributed in two contemporary 4 × 4 latin square design with a 2 × 2 factorial arrangement of treatments. experiment 1: diets were formulated with 60% of concentrate level, and two sugarcane genotypes (high or low ndfd) either freshly cut or as silage ...201728265975
species identification of streptococcus bovis group isolates causing bacteremia: a comparison of two maldi-tof ms systems.this study compared two maldi-tof ms systems (biotyper and vitek ms) on clinical streptococcus bovis group isolates (n=66). the vitek ms gave fewer misidentifications and a higher rate of correct identifications than the biotyper. only the identification of s. lutetiensis by the vitek ms was reliable. additional optimization of the available system databases is needed.201728262370
colorectal neoplasm in cases of clostridium septicum and streptococcus gallolyticus subsp. gallolyticus bacteraemia.bacteremia with clostridium septicum (cs) and streptococcus gallolyticus subsp. gallolyticus (sgg) have both been associated with colorectal neoplasms (crn) and colonoscopic examination is advised, however the differences and similarities in colorectal findings are not well known.201728236516
[significance of bacteria detection with filter paper method on diagnosis of diabetic foot wound infection].objective: to evaluate the significance of bacteria detection with filter paper method on diagnosis of diabetic foot wound infection. methods: eighteen patients with diabetic foot ulcer conforming to the study criteria were hospitalized in liyuan hospital affiliated to tongji medical college of huazhong university of science and technology from july 2014 to july 2015. diabetic foot ulcer wounds were classified according to the university of texas diabetic foot classification (hereinafter referre ...201728219141
nutritional models of experimentally-induced subacute ruminal acidosis (sara) differ in their impact on rumen and hindgut bacterial communities in dairy cows.effects of subacute ruminal acidosis (sara) challenges on the bacteria in rumen fluid, cecal digesta, and feces of dairy cows were determined using 16s rrna gene pyrosequencing and real-time quantitative pcr. six non-lactating holstein cows with cannulas in the rumen and cecum were used in a 3 × 3 latin square arrangement of treatments. during the first 3 wk of each experimental period, cows received a control diet containing 70% forages on a dry matter (dm) basis. in wk 4 of each period, cows r ...201628179895
effect of biochanin a on corn grain (zea mays) fermentation by bovine rumen amylolytic bacteria.the objective was to determine the effect of biochanin a (bca), an isoflavone produced by red clover (trifolium pratense l.), on corn fermentation by rumen micro-organisms.201728055130
relationship between enterococcus faecalis infective endocarditis and colorectal neoplasm: preliminary results from a cohort of 154 patients.the association between streptococcus bovis group infective endocarditis and colorectal neoplasm (crn) is well-known. however, no studies have assessed the association between enterococcus faecalis infective endocarditis (efie) and crn. we aimed to determine whether the prevalence of crn is higher in patients with efie and an unclear source of infection than in patients with efie and a known source of infection or in the general population.201727916708
two cases of proteinase 3-anti-neutrophil cytoplasmic antibody (pr3-anca)-related nephritis in infectious endocarditis.we herein report two cases of proteinase 3-anti-neutrophil cytoplasmic antibody (pr3-anca)-related nephritis in infectious endocarditis. in both cases, the patients were middle-aged men with proteinuria and hematuria, hypoalbuminemia, decreased kidney function, anemia, elevated c-reactive protein (crp) levels, and pr3-anca positivity. each had bacteremia, due to enterococcus faecium in one and streptococcus bovis in the other. one patient received aortic valve replacement therapy for aortic regu ...201727904114
cloning and characterization of the glutamate dehydrogenase gene in streptococcus bovis.streptococcus bovis, an etiologic agent of rumen acidosis in cattle, is a rumen bacterium that can grow in a chemically defined medium containing ammonia as a sole source of nitrogen. to understand its ability to assimilate inorganic ammonia, we focused on the function of glutamate dehydrogenase. in order to identify the gene encoding this enzyme, we first amplified an internal region of the gene by using degenerate primers corresponding to hexameric family i and nad(p)(+) binding motifs. subseq ...201627878891
[presence of streptococcus bovis in urine samples from patients experiencing symptoms of urinary tract].given the relevance of proper clinical validation of streptococcus bovis, we here consider revising its presence in urine samples in order to determine its relative frequency and the pattern of antibiotic susceptibility. the susceptibility to antibiotics of 91 isolates of s. bovis from urine samples was retrospectively reviewed over a period of 4 years (2012-2015). the mean age of patients was 55 years, 81% of whom were women and 37.4% were hospitalized patients suffering from urological disease ...201627838157
effects of the dose and viability of saccharomyces cerevisiae. 1. diversity of ruminal microbes as analyzed by illumina miseq sequencing and quantitative pcr.this study was conducted to examine effects of the dose and viability of supplemental saccharomyces cerevisiae on the ruminal fermentation and bacteria population and the performance of lactating dairy cows. four ruminally cannulated lactating cows averaging 284±18d in milk were assigned to 4 treatments arranged in a 4×4 latin square design with four 21-d periods. cows were fed a total mixed ration containing 41.7% corn silage, 12.1% brewer's grains, and 46.2% concentrate on a dry matter basis. ...201727837973
potential influence of dairy propionibacteria on the growth and acid metabolism of streptococcus bovis and megasphaera elsdenii.ruminal acidosis is a prevalent disorder among dairy cows and feedlot cattle, which can significantly impair their health and productivity. this study, involving seven different strains of dairy propionibacteria, represents an in vitro investigation of the feasibility of using these organisms as direct-fed microbials to control lactic acid acumulation in the rumen. interactions between the propionibacteria, streptococcus bovis and megasphaera elsdenii were evaluated in terms of effects on lactic ...201727824275
familial adenomatous polyposis manifesting as lactococcus endocarditis: a case report and review of the association of lactococcus with underlying gastrointestinal disease.a 45-year-old male with a prosthetic aortic valve presented to the hospital with several months of generalized malaise. on admission, he was noted to have anemia of unclear etiology and subsequently became febrile with multiple blood cultures growing lactococcus garvieae. inpatient workup was concerning for infectious endocarditis (ie) secondary to lactococcus. the patient was discharged home with appropriate antimicrobial therapy; however, he was readmitted for persistent, symptomatic anemia an ...201627818810
significant correlation between the infant gut microbiome and rotavirus vaccine response in rural ghana.rotavirus (rv) is the leading cause of diarrhea-related death in children worldwide and 95% of rv-associated deaths occur in africa and asia where rv vaccines (rvvs) have lower efficacy. we hypothesize that differences in intestinal microbiome composition correlate with the decreased rvv efficacy observed in poor settings.201727803175
streptococcus bovis new taxonomy: does subspecies distinction matter?bacteremia with streptococcus bovis/equinus complex strains is associated with hepatobiliary disease, colorectal lesions (cl), and infective endocarditis (ie). this study addressed the clinical significance of subspecies distinction of previously designated s. bovis blood culture isolates according to the updated nomenclature. during 2002-2013, all blood culture isolates previously designated as s. bovis were recultured and identified using 16s rrna gene sequencing and maldi-tof (bruker biotyper ...201727796646
relative significances of ph and substrate starch level to roles of streptococcus bovis s1 in rumen acidosis.to clarify the relative importance of ph and substrate starch level in fermentation characteristics and regulatory mechanism of streptococcus bovis s1 in rumen acidosis, an in vitro fermentation of three levels of soluble starch (1, 3 and 9 g/l) was established with ph in the media were maintained constant at 5.5 or 6.5. the results showed that the dominant product of s. bovis s1 was lactate at both ph, the production depended on the starch level, and more lactate was produced at ph 6.5 than tha ...201627655587
effects of glucose and starch on lactate production by newly isolated streptococcus bovis s1 from saanen goats.when ruminants are fed high-concentrate diets, streptococcus bovis proliferates rapidly and produces lactate, potentially causing rumen acidosis. understanding the regulatory mechanisms of the metabolism of this species might help in developing dietary strategies to alleviate rumen acidosis. s. bovis strain s1 was newly isolated from the ruminal fluid of saanen dairy goats and then used to examine the effects of glucose and starch on bacterial metabolism and gene regulation of the organic acid-p ...201627474714
phylogenetic, epidemiological and functional analyses of the streptococcus bovis/streptococcus equinus complex through an overarching mlst scheme.the streptococcus bovis/streptococcus equinus complex (sbsec) comprises seven (sub)species classified as human and animal commensals, emerging opportunistic pathogens and food fermentative organisms. changing taxonomy, shared habitats, natural competence and evidence for horizontal gene transfer pose difficulties for determining their phylogeny, epidemiology and virulence mechanisms. thus, novel phylogenetic and functional classifications are required. an sbsec overarching multi locus sequence t ...201627329036
streptococcus bovis endocarditis: epidemiological differences depending on geographical source. 201627297864
induction of subacute ruminal acidosis affects the ruminal microbiome and epithelium.subacute ruminal acidosis (sara) negatively impacts the dairy industry by decreasing dry matter intake, milk production, profitability, and increasing culling rate and death loss. six ruminally cannulated, lactating holstein cows were used in a replicated incomplete latin square design to determine the effects of sara induction on the ruminal microbiome and epithelium. experimental periods were 10 days with days 1-3 for ad libitum intake of control diet, followed by 50% feed restriction on day 4 ...201627242724
streptococcus bovis/s. equinus complex septicemia in a group of calves following intramuscular vaccination.organisms previously classified as streptococcus bovis (i.e., the s. bovis/s. equinus complex) are common in cattle feces, but may also act as opportunistic pathogens. in the current work, streptococcus infantarius subsp. coli, a member of this complex, was associated of a cluster of calves that died within hours of injection with a modified live viral vaccine. within 12 h of vaccination of 46 calves at a cow/calf operation, 4 calves had died, 3 calves were ill, and 1 unvaccinated cow was dead. ...201627216720
associated factors in streptococcus bovis bacteremia and colorectal cancer.reports suggest that between 25% and 80% of patients with streptococcus bovis/gallolyticus bacteremia have concomitant colorectal tumors. this retrospective study was aimed to identify associations between clinical characteristics and a finding of colorectal neoplasm in patients with s. bovis bacteremia who had colonoscopy examination. we retrospectively reviewed the records of patients with s. bovis bacteremia from kaohsiung chang gung memorial hospital, kaohsiung, taiwan, between january 2004 ...201627185602
the causative agents in infective endocarditis: a systematic review comprising 33,214 cases.infective endocarditis (ie) incidence remains high with considerable fatality rates; guidelines for prophylaxis against ie are currently under review in some settings which highlights the importance of maintaining up-to-date epidemiological estimates about the most common microbial causes. the objective of this systematic review, following prisma guidelines, was to identify the most common microbial causes of ie in recent years. medline was searched from january 1, 2003 to march 31, 2013 for all ...201627170145
photoinactivation of eight health-relevant bacterial species: determining the importance of the exogenous indirect mechanism.it is presently unknown to what extent the endogenous direct, endogenous indirect, and exogenous indirect mechanisms contribute to bacterial photoinactivation in natural surface waters. in this study, we investigated the importance of the exogenous indirect mechanism by conducting photoinactivation experiments with eight health-relevant bacterial species (bacteroides thetaiotaomicron, campylobacter jejuni, enterococcus faecalis, escherichia coli k12, e. coli o157:h7, salmonella enterica serovar ...201627121126
transport of a fluorescent analogue of glucose (2-nbdg) versus radiolabeled sugars by rumen bacteria and escherichia coli.fluorescent tracers have been used to measure solute transport, but transport kinetics have not been evaluated by comparison of radiolabeled tracers. using streptococcus equinus jb1 and other bacteria, the objective of this study was to determine if a fluorescent analogue of glucose (2-nbdg) would be transported with the same kinetics and transporters as [(14)c]glucose. we uniquely modified a technique for measuring transport of radiolabeled tracers so that transport of a fluorescent tracer (2-n ...201627096355
clinical interest of streptococcus bovis isolates in urine.streptococcus bovis includes variants related to colorectal cancer and non-urinary infections. its role as urinary pathogen is unknown. our objective was to assess the presence of urinary infection by s. bovis, analysing the patients and subsequent clinical course.201627084880
effects of streptococcus bovis isolated from bovine rumen on the fermentation characteristics and nutritive value of tanzania grass silage.this study aimed to evaluate the effects of streptococcus bovis on the fermentation characteristics and nutritive value of tanzania grass silage. tanzania grass was chopped and left untreated (u) or treated with streptococcus bovis jb1 at 1 × 10(6) colony-forming units per gram (cfu/g) of fresh forage or streptococcus bovis hc5 at 1 × 10(6) cfu/g of fresh forage and packed into sixtuplicate laboratory silos. the largest number of enterobacteria, molds and yeast (m&y) occurred in untreated silage ...201627073806
simultaneous saccharification and fermentation (ssf) of jackfruit seed powder (jfsp) to l-lactic acid and to polylactide polymer.a newly isolated amylolytic lactic acid bacterium, streptococcus equinus, was used for the production of l-lactic acid from jackfruit seed powder (jfsp) by simultaneous saccharification and fermentation (ssf). after optimization of shake flask fermentation by a response surface box-behnken design, the maximum lactate titer was 109g/l from 200g/l jackfruit seed powder. amberlite ira67, a weak base resin, was used to recover pure lactic acid from fermented broth and subsequently used for the synth ...201627005791
streptococcus bovis septic arthritis and osteomyelitis: a report of 21 cases and a literature review.the streptococcus bovis group (sbg) is a well-known cause of endocarditis, but its role in osteoarticular infections (oais) has not been well described.201626992635
lactic acid bacteria in total mixed ration silage containing soybean curd residue: their isolation, identification and ability to inhibit aerobic deterioration.we investigated the effects of the predominant lactic acid bacteria (lab) on the fermentation characteristics and aerobic stability of total mixed ration (tmr) silage containing soybean curd residue (sc-tmr silage). the sc-tmr materials were ensiled in laboratory silos for 14 or 56 days. lab predominant in sc-tmr silage were identified (exp. 1). lactobacillus fermentum (l. fermentum) and streptococcus bovis (s. bovis) were found in the untreated materials, leuconostoc pseudomesenteroides (l. pse ...201626949952
effect of stage of lactation on the immune competence of goat mammary gland.the present research was undertaken to evaluate the effect of stage of lactation on immune competence of the goat mammary gland. the prevalence and etiology of intramammary infections and cytokine pattern were studied in individual caprine milk during lactation. a total of 1,200 caprine milk samples were collected during early, mid, and late lactation (less than 40 d, from 110 to 130 d, and more than 200 d in lactation, respectively) and analyzed for milk quality, bacterial cell load, and cytoki ...201626947302
an update on the streptococcus bovis group: classification, identification, and disease associations.the streptococcus bovis group has undergone significant taxonomic changes over the past 2 decades with the advent of new identification methods with higher discriminatory power. although the current classification system is not yet embraced by all researchers in the field and debate remains over the performance of molecular techniques for identification to the species level within the group, important disease associations for several members of the group have been clarified. here, we provide a b ...201626912760
native valve streptococcus bovis endocarditis and refractory transfusion dependent iron deficiency anaemia associated with concomitant carcinoma of the colon: a case report and review of the literature.streptococcus bovis is found as a commensal organism in human gut and may become opportunistically pathogenic. infective endocarditis is one of the commonest modes of presentation of this infection. the association between streptococcus bovis endocarditis and colorectal cancer is well recognized. we report a case of streptococcus bovis endocarditis along with a refractory iron deficiency anaemia associated with concomitant carcinoma of ascending colon in a 63-year-old male. cooccurrence of these ...201626881154
diagnosis and treatment of childhood meningitis caused by streptococcus bovis group.streptococcus bovis is a rare, but important cause of bacterial meningitis in children. since its discovery in the early 1970s, the pathogen has undergone multiple taxonomic changes producing four distinct subspecies today, the most prevalent of which is s. gallolyticus subsp pasteurianus in infants and children. while initially reported as sporadic case reports, there is a growing body of literature documenting invasive disease primarily in neonates and infants clinically indistinguishable from ...201626879740
gut microbiota imbalance and colorectal cancer.the gut microbiota acts as a real organ. the symbiotic interactions between resident micro-organisms and the digestive tract highly contribute to maintain the gut homeostasis. however, alterations to the microbiome caused by environmental changes (e.g., infection, diet and/or lifestyle) can disturb this symbiotic relationship and promote disease, such as inflammatory bowel diseases and cancer. colorectal cancer is a complex association of tumoral cells, non-neoplastic cells and a large amount of ...201626811603
streptococcus bovis endocarditis: update from a multicenter registry.infective endocarditis (ie) due to streptococcus bovis has been classically associated with elderly patients, frequently involving >1 valve, with large vegetations and high embolic risk, which make it a high-risk group. our aim is to analyze the current clinical profile and prognosis of s bovis ie episodes, in comparison to those episodes caused by viridans group streptococci and enterococci.201626699595
an improved selex technique for selection of dna aptamers binding to m-type 11 of streptococcus pyogenes.streptococcus pyogenes is a clinically important pathogen consisting of various serotypes determined by different m proteins expressed on the cell surface. the m type is therefore a useful marker to monitor the spread of invasive s. pyogenes in a population. serotyping and nucleic acid amplification/sequencing methods for the identification of m types are laborious, inconsistent, and usually confined to reference laboratories. the primary objective of this work is to develop a technique that ena ...201626678795
ceftaroline activity tested against viridans group streptococci from us hospitals.a total of 840 clinically relevant viridans group streptococci (vgs) isolates (1/patient episode) were collected from 71 us medical centers in 2013-2014. these organisms were tested for susceptibility by reference broth microdilution methods against ceftaroline and selected comparator agents. all isolates were speciated by matrix-assisted laser desorption ionization-time-of-flight (maldi-tof) mass spectrometry and were primarily from skin/soft tissue (32.6%) and bloodstream (32.3%) infections. c ...201626658313
divergent utilization patterns of grass fructan, inulin, and other nonfiber carbohydrates by ruminal microbes.fructans are an important nonfiber carbohydrate in cool season grasses. their fermentation by ruminal microbes is not well described, though such information is needed to understand their nutritional value to ruminants. our objective was to compare kinetics and product formation of orchardgrass fructan (phlein; phl) to other nonfiber carbohydrates when fermented in vitro with mixed or pure culture ruminal microbes. studies were carried out as randomized complete block designs. all rates given ar ...201626601577
streptococcus bovis bacteremia in neonates in a predominantly hispanic population.streptococcus bovis bacteremia has been associated with gastrointestinal diseases, especially colon cancer, neoplastic colon polyps, and other malignancies of the gi tract in adults. sporadic cases of s. bovis disease have also been reported in neonates and young infants. although uncommon, s. bovis infection can cause fulminant neonatal sepsis and meningitis.201526579502
high concentration of red clay as an alternative for antibiotics in aquaculture.the use of antibiotics in aquaculture raises environmental and food safety concerns because chronic exposure of an aquatic ecosystem to antibiotics can result in the spread of antibiotic resistance, bioaccumulation of antibiotics in the organisms, and transfer of antibiotics to humans. in an attempt to overcome these problems, high-concentration red clay was applied as an alternative antibiotic against the following common fish pathogens: aeromonas salmonicida, vibrio alginolyticus, and streptoc ...201626528535
streptococcus bovis infectious endocarditis and occult gastrointestinal neoplasia: experience with 25 consecutive patients treated surgically.to assess the prevalence of gastrointestinal neoplasia in patients with streptococcus bovis infectious endocarditis we performed a retrospective cohort analysis of all episodes of s. bovis infectious endocarditis treated at our institution between january 2000 through december 2014. twenty-five patients were identified for this purpose. 12/25 patients received colonoscopy and 1/25 of the patients was assessed with ct colonography. of the 13 who underwent colonic assessment, 11 were diagnosed wit ...201526473016
[streptococcus gallolyticus (ex s. bovis) bacteremia and its relationship with colonic or hepatobiliary disease and endocarditis].bacteremia due to streptococcus bovis (now s. gallolyticus) has been traditionally associated to colon or hepatobiliar disease and endocarditis but there is no information on this matter in chile.201526436787
exploring the in vitro thrombolytic potential of streptokinase-producing β-hemolytic streptococci isolated from bovine milk.the aim of this study was to isolate and characterize streptokinase-producing β-hemolytic streptococcus sp. from bovine milk. a total of 50 milk samples were collected randomly from different breeds of cow and goat (vellore, tamil nadu, india). the samples were characterized and screened for streptokinase-producing isolates using microbial and biochemical analysis. about 97 colonies were isolated from milk samples showing hemolytic patterns of α (19.6%), β (24.7%) and γ (55.6 %). out of 20β-hemo ...201526377134
colonic carriage of streptococcus bovis and colorectal neoplasia: a prospective 17-year longitudinal case-control study.patients with streptococcus bovis infective endocarditis have an increased prevalence of advanced colonic neoplasia; however, an association with fecal carriage of the organism is unclear. s. bovis has been shown to promote colonic neoplasia in preclinical studies. the aim of this study was to examine the effect of s. bovis fecal carriage on the long-term risk of colonic neoplasia.201526340165
streptococcus gallolyticus meningitis in adults: report of five cases and review of the literature.we describe the incidence and patient characteristics of streptococcus gallolyticus meningitis. we identified s. gallolyticus meningitis in a nationwide cohort of patients with community-acquired bacterial meningitis, and performed a systematic review and meta-analysis of all reported adult cases in the literature. five cases were identified (0.3%) in a cohort of 1561 episodes of bacterial meningitis. in one patient, bowel disease (colon polyps) was identified as a predisposing condition for s.  ...201526314916
effect of diet fermentability and unsaturated fatty acid concentration on recovery from diet-induced milk fat depression.diet-induced milk fat depression is caused by highly fermentable and high-unsaturated fatty acid (fa) diets, and results in reduced milk fat concentration and yield, reduced de novo fa, and increased trans isomers of the alternate biohydrogenation pathways. the hypothesis of the current experiment was that a diet higher in fermentability and lower in unsaturated fa (ufa) would accelerate recovery compared with a high-ufa and lower-fermentability diet. eight ruminally cannulated and 9 noncannulat ...201526298764
effect of starch source (corn, oats or wheat) and concentration on fermentation by equine faecal microbiota in vitro.the goal was to determine the effect of starch source (corn, oats and wheat) and concentration on: (i) total amylolytic bacteria, group d gram-positive cocci (gpc), lactobacilli and lactate-utilizing bacteria, and (ii) fermentation by equine microbiota.201526255645
commentary on "streptococcus bovis bacteremia: association with gastrointestinal and liver disease in a predominantly hispanic population". 201526192940
streptococcus bovis bacteremia: association with gastrointestinal and liver disease in a predominantly hispanic population.streptococcus bovis bacteremia has been associated with gastrointestinal diseases, especially colon cancer, neoplastic colon polyps, and other malignancies of the gastrointestinal tract. studies have suggested an association with chronic liver parenchymal and biliary tract disorders. we report a series of patients with s. bovis bacteremia in a county hospital in a us city on the border of mexico to examine the demographic and clinical associations.201526192939
challenges in the production of itaconic acid by metabolically engineered escherichia coli.metabolic engineering allows the production of a variety of high-value chemicals in heterologous hosts. for example, itaconic acid (ia) has been produced in several microorganisms, such as escherichia coli, aspergillus niger, and synechocystis sp. through the expression of cis-aconitate decarboxylase gene (cad) from aspergillus terreus. recently, we showed that inactivation of the isocitrate dehydrogenase gene and overexpression of the aconitase gene dramatically enhanced the production levels o ...201526176321
in situ identification and quantification of starch-hydrolyzing bacteria attached to barley and corn grain in the rumen of cows fed barley-based diets.cereal grains rich in starch are widely used to meet the energy demands of high-producing beef and dairy cattle. bacteria are important players in starch digestion in the rumen, and thus play an important role in the hydrolysis and fermentation of cereal grains. however, our understanding of the composition of the rumen starch-hydrolyzing bacteria (shb) is limited. in this study, bodipy fl dq starch staining combined with fluorescence in situ hybridization (fish) and quantitative fish were appli ...201526142428
prosthetic endocarditis caused by streptococcus bovis group. 201526136389
rapid changes in key ruminal microbial populations during the induction of and recovery from diet-induced milk fat depression in dairy cows.the ruminant provides a powerful model for understanding the temporal dynamics of gastrointestinal microbial communities. diet-induced milk fat depression (mfd) in the dairy cow is caused by rumen-derived bioactive fatty acids, and is commonly attributed to the changes in the microbial population. the aim of the present study was to determine the changes occurring in nine ruminal bacterial taxa with well-characterised functions, and abundance of total fungi, ciliate protozoa and bacteria during ...201526123320
c-terminal-oriented immobilization of enzymes using sortase a-mediated technique.in the present study, sortase a-mediated immobilization of enzymes was used for the preparation of immobilized enzymes. thermobifida fusca yx β-glucosidase (bgl) or streptococcus bovis 148 α-amylase (amya) were produced with c-terminal sortase a recognition sequences. the resulting fusion proteins were successfully immobilized on nanoparticle surfaces using sortase a. some properties (activity, stability, and reusability) of the immobilized fusion proteins were evaluated. both immobilized bgl an ...201526098063
late-onset streptococcus pasteurianus sepsis in a preterm baby in a neonatal intensive care unit.apnea, cyanosis, lethargy and prolongation in capillary filling time developed on the postnatal 37(th) day in a preterm baby who was born at the 30(th) gestational week with a birth weight of 1 300 g. acute phase reactants and immature/total neutrophil count ratio were found to be high. the patient who was diagnosed with sepsis was successfully treated with meropenem which was started empirically. in his blood culture streptococcus pasteurianus grew. s. pasteurianus is in the subgroup of strepto ...201426078653
differences between endocarditis caused by streptococcus bovis and enterococcus spp. and their association with colorectal cancer.streptococcus bovis group and enterococcus spp. share phenotypic characteristics and intestinal habitat. both have been associated with endocarditis and colorectal neoplasm (crn). we studied all cases of endocarditis diagnosed between 1988 and 2014 in our centre and caused by s. bovis (109, 48.8 % of the bacteremia) and by enterococcus spp. (36, 3.4 % of the bacteremia). patients were seen until death or during a long-term follow-up, in order to rule out a concomitant crn. the 109 cases of s. bo ...201526017665
association of streptococcus bovis presence in colonic content with advanced colonic lesion.to prospectively examine the association between presence of streptococcus bovis (s. bovis) in colonic suction fluid and the endoscopic findings on colonoscopy.201525987793
the clinical epidemiology and malignancies associated with streptococcus bovis biotypes in 506 cases of bloodstream infections.to determine the incidence of streptococcus bovis (sb) biotypes causing bacteraemia and associated malignancies.201525982024
pulmonary endarterectomy for pulmonary hypertension from septic emboli.pulmonary endarterectomy (pea) can significantly increase long-term survival in patients with chronic thromboembolic pulmonary hypertension; however, the role of pea for chronic thromboembolic pulmonary hypertension due to pulmonary valve endocarditis is controversial. a critically ill 61-year-old man with intractable right ventricular heart failure was found to have chronic thromboembolic pulmonary hypertension due to streptococcus bovis pulmonary valve endocarditis and underwent successful pul ...201525952218
antimicrobial activities of chicken β-defensin (4 and 10) peptides against pathogenic bacteria and fungi.host defense peptides (hdps) are small cationic peptides found in several organisms. they play a vital role in innate immunity response and immunomodulatory stimulation. this investigation was designed to study the antimicrobial activities of β-defensin peptide-4 (savbd-4) and 10 (savbd-4) derived from chickens against pathogenic organisms including bacteria and fungi. ten bacterial strains and three fungal species were used in investigation. the results showed that the savbd-10 displayed a high ...201525941665
draft genome sequence of streptococcus equinus (streptococcus bovis) hc5, a lantibiotic producer from the bovine rumen.streptococcus equinus (streptococcus bovis) hc5 is a bacteriocinogenic lactic acid bacterium with simple growth requirements. the draft genome sequence of s. equinus hc5 consists of 1,846,241 bp, with a g+c content of 37.04%. in silico analysis indicated that s. equinus hc5 might be useful to control bacteria that are detrimental to livestock animals.201525745007
neonatal invasive streptococcus gallolyticus subsp. pasteurianus infection with delayed central nervous system complications.group d streptococci are known to cause newborn septicemia and meningitis, but the streptococcus bovis group strains rarely cause serious neonatal infections in korea. central nervous system (cns) complications of neonatal s. bovis group infection have rarely been reported. in adults, s. bovis group strains cause bacteremia and endocarditis, and are associated with gastrointestinal malignancy. however, only a few studies have reported meningitis and septicemia in infants. here, we describe a cas ...201525729397
suicin 3908, a new lantibiotic produced by a strain of streptococcus suis serotype 2 isolated from a healthy carrier pig.while streptococcus suis serotype 2 is known to cause severe infections in pigs, it can also be isolated from the tonsils of healthy animals that do not develop infections. we hypothesized that s. suis strains in healthy carrier pigs may have the ability to produce bacteriocins, which may contribute to preventing infections by pathogenic s. suis strains. two of ten s. suis serotype 2 strains isolated from healthy carrier pigs exhibited antibacterial activity against pathogenic s. suis isolates. ...201525659110
streptococcus bovis endocarditis: analysis of cases between 2005 and 2014.streptococcus bovis is a classical etiology of endocarditis and is associated with colonic lesions. no series of cases from brazil has been described.201525636194
streptococcus gallolyticus subsp. pasteurianus infection in twin infants.streptococcus gallolyticus subsp. pasteurianus, previously known as streptococcus bovis biotype ii.2, is an uncommon pathogen in neonates. nevertheless, it can cause severe neonatal sepsis and meningitis often clinically indistinguishable from those caused by group b streptococci and has been associated with considerable morbidity. we report the first known cases of s. gallolyticus subsp. pasteurianus infection in twin infants.201525609731
acquisition through horizontal gene transfer of plasmid psma198 by streptococcus macedonicus aca-dc 198 points towards the dairy origin of the species.streptococcus macedonicus is an intriguing streptococcal species whose most frequent source of isolation is fermented foods similarly to streptococcus thermophilus. however, s. macedonicus is closely related to commensal opportunistic pathogens of the streptococcus bovis/streptococcus equinus complex.201525584532
five cases of recurrent meningitis associated with chronic strongyloidiasis.although meningitis secondary to chronic strongyloidiasis is a rare complication, it is associated with a high mortality rate. recurrent meningitis can occur if the underlying parasitic infection is left untreated. we report five cases of recurrent meningitis related to chronic strongyloidiasis that were associated with human t-lymphotropic virus type 1 (htlv-1) infection. common causative organisms are escherichia coli, streptococcus bovis, and klebsiella pneumonia. one patient died during the ...201525548379
renal involvements in reported cases of streptococcus bovis endocarditis. 201525528307
failure of early diagnosis of infective endocarditis in japan--a retrospective descriptive analysis.infective endocarditis (ie) is a severe disease with high morbidity and mortality, and these can be exacerbated by delay in diagnosis. we investigated ie diagnosis in japan with the emphasis on the delay in diagnosis and its cause and implications. we conducted a retrospective study on 82 definite ie patients at kobe university hospital from april 1, 2008, through march 31, 2013. we reviewed charts of the patients for data such as causative pathogens, prescription of inappropriate antibiotic use ...201425501088
production of itaconic acid in escherichia coli expressing recombinant α-amylase using starch as substrate.several studies on fermentative production of a vinyl monomer itaconic acid from hydrolyzed starch using aspergillus terreus have been reported. herein, we report itaconic acid production by escherichia coli expressing recombinant α-amylase, using soluble starch as its sole carbon source. to express α-amylase in e. coli, we first constructed recombinant plasmids expressing α-amylases by using cell surface display technology derived from two amylolytic bacteria, bacillus amyloliquefaciens nbrc 15 ...201525468427
[recurrent meningitis due to anatomical defects: the bacteria indicates its origin].recurrent meningitis is a rare disease. anatomical abnormalities and immunodeficiency states are predisposing factors. four cases, in which immunodeficiency was excluded, are presented. the causal microorganism led to the detection of the anatomical defect responsible for the recurrences.201525446794
effects of nitrate addition to a diet on fermentation and microbial populations in the rumen of goats, with special reference to selenomonas ruminantium having the ability to reduce nitrate and nitrite.this study investigated the effects of dietary nitrate addition on ruminal fermentation characteristics and microbial populations in goats. the involvement of selenomonas ruminantium in nitrate and nitrite reduction in the rumen was also examined. as the result of nitrate feeding, the total concentration of ruminal volatile fatty acids decreased, whereas the acetate : propionate ratio and the concentrations of ammonia and lactate increased. populations of methanogens, protozoa and fungi, as esti ...201525439583
cloning, expression, and purification of a new antibacterial substance gene from larvae of musca domestica (diptera: muscidae).musca domestica l. (diptera: muscidae), the housefly, exhibits unique immune defenses and can produce antibacterial substances upon stimulation with bacteria. on the basis of the cdna library constructed using the suppression subtractive hybridization method, a 1188-bp antibacterial substance gene, which we named as566, was amplified by rapid amplification of cdna ends from m. domestica larva stimulated with salmonella pullorum (enterobacteriaceae: salmonella). in this study, the full-length as5 ...201425434038
efficient co-displaying and artificial ratio control of α-amylase and glucoamylase on the yeast cell surface by using combinations of different anchoring domains.recombinant yeast strains that display heterologous amylolytic enzymes on their cell surface via the glycosylphosphatidylinositol (gpi)-anchoring system are considered as promising biocatalysts for direct ethanol production from starchy materials. for the effective hydrolysis of these materials, the ratio optimization of multienzyme activity displayed on the cell surface is important. in this study, we have presented a ratio control system of multienzymes displayed on the yeast cell surface by u ...201525432675
is streptococcus bovis a urinary pathogen?the streptococcus bovis group (sbg) comprises several microorganisms associated with human infections. they have been associated with bacteremia, endocarditis, biliary tract infection, meningitis, and colorectal cancer, but their role as urinary pathogens is not well known. the objective of this investigation was to discover the incidence and clinical significance of the bacteriuria associated with this complex. a retrospective analysis of all adult patients with bacteriuria caused by sbg during ...201525416160
effect of fiber digestibility and conservation method on feed intake and the ruminal ecosystem of growing steers.fiber digestibility is an important factor regulating dmi in ruminants. additionally, the ensiling process can also affect digestibility and chemical composition of the forage. the objective of this study was to investigate effects of sugarcane ndf digestibility (ndfd) and conservation method on intake, rumen kinetics, and the ruminal ecosystem of steers. eight ruminally cannulated nellore steers (275±22 kg bw) were used in a replicated 4×4 latin square design with a 2×2 factorial arrangement of ...201425414108
draft genome sequences of streptococcus bovis strains atcc 33317 and jb1.we report the draft genome sequences of streptococcus bovis strain atcc 33317 (cvm42251) isolated from cow dung and strain jb1 (cvm42252) isolated from a cow rumen in 1977. the strains were sequenced using the genome sequencer flx 454 system. the genome sizes are approximately 2 mb and 2.2 mb, respectively.201425301652
active dry saccharomyces cerevisiae can alleviate the effect of subacute ruminal acidosis in lactating dairy cows.the objective of the study was to determine the effect of active dry saccharomyces cerevisiae (adsc) supplementation on dry matter intake, milk yield, milk components, ruminal ph, and microbial community during a dietary regimen that leads to subacute ruminal acidosis (sara). sixteen multiparous, rumen-cannulated lactating holstein cows were randomly assigned to 1 of 2 dietary treatments that included adsc (biomate; ab vista, marlborough, uk; 8 × 10(10) cfu/head per day) or control. during wk 1 ...201425282426
genomics, evolution, and molecular epidemiology of the streptococcus bovis/streptococcus equinus complex (sbsec).the streptococcus bovis/streptococcus equinus complex (sbsec) is a group of human and animal derived streptococci that are commensals (rumen and gastrointestinal tract), opportunistic pathogens or food fermentation associates. the classification of sbsec has undergone massive changes and currently comprises 7 (sub)species grouped into four branches based on sequences identities: the streptococcus gallolyticus, the streptococcus equinus, the streptococcus infantarius and the streptococcus alactol ...201525233845
[streptococcus bovis endocarditis as a sign of colon cancer: case report].streptococcus bovis is labeled in the literature as a cause of bacteremia and endocarditis, which are often associated with gastrointestinal malignancy.201425233695
[a case of streptococcus gallolyticus subsp. gallolyticus infective endocarditis with colon adenoma].an 80-year-old japanese man had a fall presented with a 3-week history of right lumbago exacerbated by body movement as well as a 1-week history of anomalous behavior and appetite loss. he visited our hospital complaining of difficulty in standing up. he had a history of mitral prolapse due to an unknown rupture of the chordae tendineae 3 years earlier, which resulted in moderate mitral valve regurgitation and atrial fibrillation. upon visiting the hospital, he had petechial hemorrhage and jaund ...201425199383
childhood meningitis caused by streptococcus bovis group: clinical and biologic data during a 12-year period in france.bacterial meningitis (bm) is a major cause of morbidity and mortality in children. sporadic cases of streptococcus bovis have been described in neonates and infants. to assess the epidemiologic, clinical and biologic characteristics of this meningitis, we used the french surveillance network for bm in children.201525144798
streptococcus bovis endocarditis as first clinical expression of an occult colorectal neoplasm. 201525060213
acute septicemia caused by streptococcus gallolyticus subsp. pasteurianus in turkey poults.streptococcus gallolyticus, previously known as streptococcus bovis biotypes i and ii/2, is a well-known cause of sepsis and meningitis in humans and birds. the present case report describes an outbreak of fatal septicemia associated with s. gallolyticus subsp. pasteurianus (s. bovis biotype ii/2) in 11 turkey flocks in pennsylvania between 2010 and 2013. affected poults were 2-3 wk of age. major clinical observation was sudden increase in mortality among turkey poults without any premonitory cl ...201425055641
effects of soybean small peptides on rumen fermentation and on intestinal and total tract digestion of luxi yellow cattle.four luxi beef cattle (400±10 kg) fitted with ruminal, duodenal and ileal cannulas were used in a 4×4 latin square to assess the effects of soybean small peptide (ssp) infusion on rumen fermentation, diet digestion and flow of nutrient in the gastrointestinal tract. the ruminal infusion of ssp was 0 (control), 100, 200 and 300 g/d. ruminal ssp infusion linearly (p<0.01) and quadratically (p<0.01) increased microbial protein synthesis and rumen ammonia-n concentration. concentrations of total vol ...201325049708
streptococcus bovis bacteremia as the initial presentation of carcinoma of the gallbladder.there is a well known association between streptococcus bovis infection and the presence of a coexisting colonic adenocarcinoma. association of s. bovis with other malignancies has only been rarely reported. we report a 77-year-old male who presented with with streptococcus bovis bacteremia in the setting of acute cholecystitis. given the association of colon adenocarcinoma and streptococcus bovis bacteremia, colonoscopy was performed prior to cholecystectomy to evaluate a possible coexisting co ...201424902466
in vitro thrombolytic activity of purified streptokinase extracted from streptococcus equinus vit_vb2 isolated from bovine milk.streptokinase (sk) is an extracellular enzyme secreted by various strains of β-hemolytic streptococci. the main focus of the current study is to evaluate the in vitro thrombolytic activity of purified sk extracted from streptococcus equinus vit_vb2 (accession no. jx406835) isolated from milk sample. the growth rate of s. equinus vit_vb2 strain was studied with ph and biomass content which has positive significant effect on enzyme yield. a temperature of 10 °c and ph of 6 was found to be optimum ...201524897956
ruminal bacterial community shifts in grain-, sugar-, and histidine-challenged dairy heifers.ruminal bacterial community composition (bcc) and its associations with ruminal fermentation measures were studied in dairy heifers challenged with combinations of grain, fructose, and histidine in a partial factorial study. holstein-friesian heifers (n=30) were randomly allocated to 5 triticale grain-based treatment groups: (1) control (no grain), (2) grain [fed at a dry matter intake (dmi) of 1.2% of body weight (bw)], (3) grain (0.8% of bw dmi) + fructose (0.4% of bw dmi), (4) grain (1.2% of ...201424881800
comparison of clinical features of left-sided infective endocarditis involving previously normal versus previously abnormal valves.native valve infective endocarditis (ie) in patients with normal valves has increased in the last decades. whether patients with normal valves present a similar prognosis to those with pathologic valves is unresolved. our aim is to describe epidemiologic and clinical differences between patients with left-sided ie and normal valves and those with native pathologic valves. we analyzed 945 consecutive episodes of ie, 435 of which involved left-sided nonprosthetic ie. they were classified into 2 gr ...201424878130
streptococcus viridians bacteraemia and colonic adenocarcinoma.even though streptococcus bacteraemia is an infrequent presentation of colonic malignancies, there is a well-established link between streptococcus bovis bacteraemia and colonic malignancies. most of the physicians are well aware of this correlation and further workup is pursued in most of the cases. however, many physicians may not be aware that other species of streptococcus viridians, other than s. bovis, can be associated with colonic malignancies. in this case report, we present a case of s ...201424876211
analysis of bacterial communities and bacterial pathogens in a biogas plant by the combination of ethidium monoazide, pcr and ion torrent sequencing.the present study investigated the changes of bacterial community composition including bacterial pathogens along a biogas plant, i.e. from the influent, to the biogas reactor and to the post-digester. the effects of post-digestion temperature and time on the changes of bacterial community composition and bacterial pathogens were also studied. microbial analysis was made by ion torrent sequencing of the pcr amplicons from ethidium monoazide treated samples, and ethidium monoazide was used to cle ...201424852413
streptococcus bovis infection and colorectal neoplasia: a meta-analysis.a meta-analysis was conducted to determine the risk associated with streptococcus bovis infection and the occurrence of colorectal neoplasia (crn). the level of risk remains unknown.201424824513
bovicin hj50-like lantibiotics, a novel subgroup of lantibiotics featured by an indispensable disulfide bridge.lantibiotics are ribosomally-synthesized and posttranslationally modified peptides with potent antimicrobial activities. discovery of novel lantibiotics has been greatly accelerated with the soaring release of genomic information of microorganisms. as a unique class ii lantibiotic, bovicin hj50 is produced by streptococcus bovis hj50 and contains one rare disulfide bridge. by using its precursor bova as a drive sequence, 16 bova-like peptides were revealed in a wide variety of species. from them ...201424821187
infective endocarditis caused by streptococcus gallolyticus subspecies pasteurianus and colon cancer.a 56-year-old man presented to our department with a 2-month history of fever and chills. he had received a mitral valvuloplasty 3 years ago. he had been administered levofloxacin for 2 months. we discontinued levofloxacin and repeated the blood cultures. bacterial blood cultures were positive and transoesophageal echocardiography revealed vegetation attached to the posterior mitral leaflet. we started the patient on intravenous antibiotic therapy for infectious endocarditis by streptococcus gal ...201424798358
inhibition of fructan-fermenting equine faecal bacteria and streptococcus bovis by hops (humulus lupulus l.) β-acid.the goals of this study were to determine if β-acid from hops (humulus lupulus l.) could be used to control fructan fermentation by equine hindgut micro-organisms, and to verify the antimicrobial mode of action on streptococcus bovis, which has been implicated in fructan fermentation, hindgut acidosis and pasture-associated laminitis (pal) in the horse.201424775300
streptococcus gallolyticus subsp. pasteurianus meningitis in an infant.streptococcus gallolyticus subsp. pasteurianus was formerly classified as s. bovis biotype ii/2, which is recognized as a rare cause of neonatal sepsis and meningitis. since the taxonomy classification change, there have not been many reports of meningitis due to s. gallolyticus subsp. pasteurianus. moreover, the pathogenesis of late onset s. gallolyticus subsp. pasteurianus meningitis in infants is unclear. here we report a case of meningitis in a 5-week-old infant with preceding diarrhea. s. b ...201424730635
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