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lactoferrin affects the adherence and invasion of streptococcus dysgalactiae ssp. dysgalactiae in mammary epithelial cells.streptococcus dysgalactiae ssp. dysgalactiae is an important causative agent of bovine mastitis worldwide. lactoferrin is an innate immune protein that is associated with many functions including immunomodulatory, antiproliferative, and antimicrobial properties. this study aimed to investigate the interactions between lactoferrin and a clinical bovine mastitis isolate, strep. dysgalactiae ssp. dysgalactiae dpc5345. initially a deliberate in vivo bovine intramammary challenge was performed with s ...201627016824
recurrent bacteremia with streptococcus dysgalactiae: a case-control study.beta-hemolytic streptococci of groups c and g, designated as streptococcus dysgalactiae (sd), can cause severe and recurring invasive infections. in this case-control study, we aimed to identify clinical and molecular risk factors for recurrence of sd bacteremia. twenty-two cases of recurrent sd bacteremia were identified, and median time between episodes was 6 months. the most frequent clinical manifestation was skin and soft tissue infection. cases and 92 controls, with single-episode sd bacte ...201626906192
transcriptome analysis of soiny mullet (liza haematocheila) spleen in response to streptococcus dysgalactiae.soiny mullet (liza haematocheila) is becoming an economically important aquaculture mugilid species in china and other asian countries. however, increasing incidences of bacterial pathogenic diseases has greatly hampered the production of the soiny mullet. deeper understanding of the soiny mullet immune system and its related genes in response to bacterial infections are necessary for disease control in this species. in this study, the transcriptomic profile of spleen from soiny mullet challenge ...201626707943
identification and characterization of cd4⁺ t-cell epitopes on gapc protein of streptococcus dysgalactiae.the gapc protein is highly conserved surface dehydrogenase among streptococcus dysgalactiae (s. dysgalactiae) and is shown to be involved in bacterial virulence. immunization of gapc protein can induce specific cd4(+) t-cell immune responses and protect against s. dysgalactiae infection. however, there are no studies to identify immunodominant cd4(+) t-cell epitopes on gapc protein. in this study, in silico mhc affinity measurement method was firstly used to predict potential cd4(+) t-cell epito ...201626657721
infection of human keratinocytes by streptococcus dysgalactiae subspecies dysgalactiae isolated from milk of the bovine udder.streptococcus dysgalactiae subsp. dysgalactiae (sdsd) are considered exclusive animal pathogens; however, a putative zoonotic upper limb cellulitis, a prosthetic joint infection and an infective endocarditis were described in humans. to unravel if bovine sdsd isolates are able to infect human cells, the adherence and internalization to human primary keratinocytes of two bovine sdsd strains isolated from milk collected from udder were analyzed. bacterial adhesion assays and confocal microscopy in ...201626655883
expression, genetic localization and phylogenic analysis of naplr in piscine streptococcus dysgalactiae subspecies dysgalactiae isolates and their patterns of adherence.streptococcus dysgalactiae, the long recognized mammalian pathogen, has currently received a major concern regarding fish bacterial infection. adhesion to host epithelial cells and the presence of wall-associated plasminogen binding proteins are prerequisites to streptococcus infection. this is the first study of the occurrence of nephritis-associated plasminogen-binding receptor (naplr) and α-enolase genes in piscine s. dysgalactiae subspecies dysgalactiae (sdsd) isolates. further characterizat ...201526425363
toxic shock syndrome: an unusual organism.streptococcal toxic shock syndrome (stss) is a rapidly fatal disease causing hypotension with multiorgan dysfunction (mods) early in the course of infection, which by definition is caused by group a streptococcus (gas). in this report, a case of toxic shock-like syndrome (tsls) in which the causative organism was not a gas was described.201526244694
renal embolism as a primary manifestation of streptococcus dysgalactiae subspecies equisimilis endocarditis in a patient with chronic aortic dissection.we report a case of renal embolism as an initial manifestation of streptococcus dysgalactiae subspecies equisimilis (sdse) endocarditis in a patient with chronic aortic dissection. a 37-year-old man who underwent total aortic arch replacement owing to aortic dissection, presented with a 3-h history of fever, chills, and acute right-sided flank pain. the endocarditis affected the native aortic valve and was complicated by a renal embolism. blood culture results were positive for sdse. intravenous ...201526110298
identification of a conserved b-cell epitope on the gapc protein of streptococcus dysgalactiae.streptococcus dysgalactiae (s. dysgalactia) gapc is a highly conserved surface dehydrogenase among the streptococcus spp., which is responsible for inducing protective antibody immune responses in animals. however, the b-cell epitope of s. dysgalactia gapc have not been well characterized. in this study, a monoclonal antibody 1f2 (mab1f2) against s. dysgalactiae gapc was generated by the hybridoma technique and used to screen a phage-displayed 12-mer random peptide library (ph.d.-12) for mapping ...201525936577
genetic diversity of geographically distinct streptococcus dysgalactiae isolates from fish.streptococcus dysgalactiae is an emerging pathogen of fish. clinically, infection is characterized by the development of necrotic lesions at the caudal peduncle of infected fishes. the pathogen has been recently isolated from different fish species in many countries. twenty s. dysgalactiae isolates collected from japan, taiwan, malaysia and indonesia were molecularly characterized by biased sinusoidal field gel electrophoresis (bsfge) using smai enzyme, and tuf gene sequencing analysis. dna sequ ...201525750757
characteristics of sheep flocks affected by streptococcus dysgalactiae arthritis. 201525724543
beta-haemolytic group a, c and g streptococcal infections in western norway: a 15-year retrospective survey.pyogenic streptococci cause significant morbidity and mortality, and the incidence of invasive group c and g streptococcal disease appears to be increasing. in this retrospective study we describe the epidemiological characteristics of invasive group a, c and g, along with non-invasive group c and g streptococcal infections in western norway from 1999 to 2013. a total of 512 invasive streptococcal infections were identified, of these 297 (58%) were group a (gas), 24 (5%) group c (gcs) and 188 (3 ...201525658557
mastitis in a neonatal filly.neonatal mastitis is a rare occurrence in the horse. this report documents a case of mastitis caused by an organism within the streptococcus dysgalactiae group in a 1-week-old paint filly.201525565717
[streptococcus dysgalactiae subspecies equisimilis bacteremic erythroderma in a pediatric patient: a case report and review of literature].infections caused by pyogenic streptococci are commons in pediatric ages. however, in the last decades there has been an increase in the isolation of no a or b streptococci. we report a case of a 6 years old girl, who presents fever for 5 days and erythroderma. streptococcus dysgalactiae subspecies equisimilis was isolated from blood cultures. she receives antibiotics for 14 days with ß-lactams with a good clinic evolution with normalization of the inflammatory parameters. this agent ß-hemolític ...201425491463
new tricks from an old cow: infective endocarditis caused by streptococcus dysgalactiae subsp. dysgalactiae.we present a case of infective endocarditis caused by streptococcus dysgalactiae subsp. dysgalactiae, a major cause of bovine mastitis and previously thought to be an animal-restricted pathogen. the patient reported no direct contact with animals, and the clinical course was severe and complicated.201525472489
production of mouse monoclonal antibody against streptococcus dysgalactiae gapc protein and mapping its conserved b-cell epitope.streptococcus dysgalactiae (s. dysgalactiae) gapc protein is a protective antigen that induces partial immunity against s. dysgalactiae infection in animals. to identify the conserved b-cell epitope of s. dysgalactiae gapc, a mouse monoclonal antibody 1e11 (mab1e11) against gapc was generated and used to screen a phage-displayed 12-mer random peptide library (ph.d.-12). eleven positive clones recognized by mab1e11 were identified, most of which matched the consensus motif tgffakk. sequence of th ...201525468796
prevalence of bacterial genotypes and outcome of bovine clinical mastitis due to streptococcus dysgalactiae and streptococcus uberis.streptococcus dysgalactiae and streptococcus uberis are common causes of clinical mastitis (cm) in dairy cows. in the present study genotype variation of s. dysgalactiae and s. uberis was investigated, as well as the influence of bacterial species, or genotype within species, on the outcome of veterinary-treated cm (vtcm). isolates of s. dysgalactiae (n = 132) and s. uberis (n = 97) were genotyped using pulsed-field gel electrophoresis. identical banding patterns were called pulsotypes. outcome ...201425427658
cellulitis-like skin eruption of purulent tenosynovitis caused by streptococcus dysgalactiae. 201525350731
streptococcus dysgalactiae subspecies equisimilis bacteraemia in an hiv-1 patient with hbv/hcv co-infections: case report and literature review.streptococcus dysgalactiae subspecies equisimilis (sdse) is a common pathogen in animals and generally considered a rare cause of infection in humans. recently, epidemiological studies demonstrated an increasing number of severe infections, including bacteraemia and endocarditis, caused by sdse, mainly in predisposed hosts, immunocompromised by underlying conditions. even though the immune status seems to play an important role in the appearance of sdse infections, this microorganism has been ra ...201425269968
distribution of virulence factors and association with emm polymorphism or isolation site among beta-hemolytic group g streptococcus dysgalactiae subspecies equisimilis.distribution of virulence factors and association with emm polymorphism or isolation site among beta-hemolytic group g streptococcus dysgalactiae subspecies equisimilis. streptococcus dysgalactiae subspecies equisimilis (sdse), the dominant human pathogenic species among group g streptococci, is the causative agent of several invasive and non-invasive diseases worldwide. however, limited information is available about the distribution of virulence factors among sdse isolates, or their associatio ...201525244428
molecular cloning, expression analysis and functional characterization of interleukin-22 in so-iny mullet, liza haematocheila.in the present study, interleukin-22 (il-22) from so-iny mullet (liza haematocheila) was identified, and its tissue expression in both healthy and streptococcus dysgalactiae-infected fish was examined. the full length cdna sequence of mullet il-22 was 1070bp, containing an open reading frame of 555bp. the deduced amino acid sequence shared high similarity (45.1-67.9%) with il-22 from other fish species. mullet il-22 also contained an il-10 family signature and four cysteine residues that were we ...201525108846
genetic characteristics of streptococcus dysgalactiae isolated from cage cultured cobia, rachycentron canadum (l.).disease outbreaks occurred during 2007-2013 in taiwan with 2.5-10% mortality among the cage cultured cobia, rachycentron canadum (l.), characterized by the presence of polyserositis, pericarditis and peritonitis. the micro-organisms isolated from internal organs were gram-positive cocci. the isolates were confirmed as streptococcus dysgalactiae by a polymerase chain reaction assay that yielded the expected specific 259 bp amplicon. additionally, partial sequence of the 16s-23s rdna intergenic sp ...201525087459
antibiotic susceptibility pattern and erythromycin resistance mechanisms in beta-hemolytic group g streptococcus dysgalactiae subspecies equisimilis isolates from central taiwan.information concerning antibiotics susceptibilities of beta-hemolytic group g streptococcus dysgalactiae subspecies equisimilis (sdse) clinical isolates in central taiwan was limited.201524856419
structural investigation of rhamnose-rich polysaccharides from streptococcus dysgalactiae bovine mastitis isolate.different rhamnose-rich polysaccharides (rrp) were identified in the cell envelope of the gram-positive bovine mastitis isolate streptococcus dysgalactiae 2023. structural investigations of the 1d and 2d nuclear magnetic resonance experiments as well as chemical analyses identified as main components l-rha and d-galnac. two main rrp were characterized, namely 1 being composed of the repeating unit {→3)-α-l-rhap-(1→2)-[α-d-galpnac-(1→3)-β-d-galpnac-(1→3)-]α-l-rhap-(1} and 2 possessing the repeat ...201424680507
fibrin-targeted plasminogen activation by plasminogen activator, pada, from streptococcus dysgalactiae.bacterial plasminogen activators differ from each other in their mechanism of plasminogen activation besides their host specificity. three-domain streptokinase (sk) and two-domain paua generate nonproteolytic active site center in their cognate partner plasminogen but their binary activator complexes are resistant to α2-antiplasmin (a2ap) inhibition causing nonspecific plasminogen activation in plasma. in contrast, single-domain plasminogen activator, staphylokinase (sak), requires proteolytic c ...201424639287
sources of streptococcus dysgalactiae in english and welsh sheep flocks affected by infectious arthritis (joint ill).in order to investigate sheep sources of streptococcus dysgalactiae in flocks affected with joint ill, 10 sheep flocks in england and wales with laboratory-confirmed cases of infectious arthritis (joint ill) caused by s dysgalactiae were visited during a disease outbreak while a further four flocks were visited during the lambing period in the year following an outbreak. a total of 5239 samples were collected for bacterial culture from 797 ewes and their 1314 lambs. s dysgalactiae was isolated f ...201424619629
current views of haemolytic streptococcal pathogenesis.increasing disease caused by beta-haemolytic streptococci indicates the need for improved understanding of pathogenesis.201424573012
systemic streptococcus dysgalactiae subspecies equisimilis infection in a yorkshire pig with severe disseminated suppurative meningoencephalomyelitis.an 18-week-old female breeding yorkshire pig displayed symptoms of astasia and subsequently died. histologically, severe disseminated suppurative meningoencephalomyelitis was detected, as were numerous myocardial microabscesses. gram-positive cocci were detected in these suppurative lesions, and these cocci reacted with an antibody against streptococcus c group species. gram-positive cocci were isolated from the liver, spleen, kidney, heart, lungs, pleural abscess and articular fluid of the righ ...201424419876
cloning and expression of a surface immunogenic protein in streptococcus dysgalactiae isolated from fish and its application in enzyme-linked immunosorbent assays to diagnose s. dysgalactiae infections in fish.lancefield group c streptococcus dysgalactiae (gcsd) causes severe necrotic lesions in the caudal peduncle in the genus seriola farmed in japan. to develop a sero-diagnostic method for gcsd infection in farmed fish, we attempted to identify a surface immunogenic protein that induces an antibody after infection with gcsd by immunoblot analysis using sera collected from infected fish. a protein obtained from sodium dodecyl sulfate (sds) extracts of gcsd was identified as s. dysgalactiae surface im ...201424131210
meningitis in a pregnant woman caused by streptococcus dysgalactiae subspecies equisimilis.infection of the central nervous system by streptococci is known to result in severe bacterial meningitis, however some strains have low pathogenic potential and affect the brain only in immunocompromised patients. here we report the first case of an otherwise healthy non immunocompromised young adult woman who developed meningitis caused by streptococcus dysgalactiae subspecies equisimilis. the patient was in the 17th week of her 3rd pregnancy. the course of the disease was quickly remittent un ...201324053027
sequence diversity of sicg among group c and g streptococcus dysgalactiae subspecies equisimilis isolates associated with human infections in western norway.streptococcal inhibitor of complement (sic) and distantly related to sic (drs) are well-characterized extracellular virulence factors produced by only a few emm types among group a streptococci. the prevalence and sequence variations of the sic-like gene (sicg) in clinical samples of group c and g streptococcus dysgalactiae subspecies equisimilis (sdse), however, have not been widely investigated. we constructed primers targeting sicg and screened 129 geographically matched and previously emm-ty ...201424019161
perianal streptococcal dermatitis due to streptococcus dysgalactiae ssp. equisimilis in a man who has sex with men. 201323845120
genotyping of streptococcus dysgalactiae strains isolated from nile tilapia, oreochromis niloticus (l.).streptococcus dysgalactiae is an emerging fish pathogen that is responsible for outbreaks of disease on fish farms around the world. recently, this bacterium was associated with an outbreak at a nile tilapia, oreochromis niloticus (l.), farm in brazil. the aim of this study was to evaluate the genetic diversity, best genotyping method and aspects of molecular epidemiology of s. dysgalactiae infections in nile tilapia farms in brazil. twenty-one isolates from four farms located in different brazi ...201423786245
identification of gram-positive cocci by use of matrix-assisted laser desorption ionization-time of flight mass spectrometry: comparison of different preparation methods and implementation of a practical algorithm for routine diagnostics.this study compared three sample preparation methods (direct transfer, the direct transfer-formic acid method with on-target formic acid treatment, and ethanol-formic acid extraction) for the identification of gram-positive cocci with matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms). a total of 156 gram-positive cocci representing the clinically most important genera, aerococcus, enterococcus, staphylococcus, and streptococcus, as well as more rare gene ...201323554198
impact of identification of streptococcus dysgalactiae subspecies equisimilis from throat cultures in an adult population.streptococcus dysgalactiae subspecies equisimilis (sdse) are isolated from the throat of patients with pharyngitis, although the clinical significance remains debated. we sought to determine the incidence and association with pharyngitis of sdse in an adult veteran population. organisms were phenotypically identified to subspecies and lancefield group, with selective 16s rrna gene sequencing. from 833 throat cultures, the overall frequency of sdse was 3.4% (64% group c and 36% group g) as compar ...201323537788
streptococcal pyrogenic exotoxin g gene in blood and pharyngeal isolates of streptococcus dysgalactiae subspecies equisimilis has a limited role in pathogenesis.streptococcus dysgalactiae subspecies equisimilis (se) causes human infections that clinically resemble infections due to streptococcus pyogenes (sp). se expresses several virulence determinants initially identified in sp, including genes encoding streptococcal pyrogenic exotoxins. se isolates from patients with toxic shock syndrome were found to harbor a gene designated spegg, which is similar to the sp pyrogenic exotoxin-g gene, termed speg. other streptococcal pyrogenic exotoxins known to exi ...201423380619
host-pathogen interactions in streptococcal immune sequelae.otherwise uncomplicated infections with streptococcus pyogenes can cause two insidious immune sequelae known as post-streptococcal glomerulonephritis (psgn) and acute rheumatic fever (arf). these diseases follow with a latency of a few weeks or months after primary infection and are responsible for high mortality and morbidity. psgn has also been linked to infections with group c streptococci of the species s. equi ssp. zooepidemicus (sesz). moreover, there are some indications that infection wi ...201323212184
haemolytic uraemic syndrome associated with bloody diarrhoea caused by streptococcus dysgalactiae. 201322955002
characterization of the bovine innate immune response in milk somatic cells following intramammary infection with streptococcus dysgalactiae subspecies dysgalactiae.the innate immune response of milk somatic cells in cows to streptococcus dysgalactiae ssp. dysgalactiae was investigated by deliberate intramammary challenge. cows were challenged with 2,500 colony-forming units of strep. dysgalactiae dpc 5435, previously isolated from a clinical mastitis case. eight of the 9 cows treated showed clinical signs of mastitis (swollen udders, increased somatic cell score, and clotted milk) within 1 wk of challenge. messenger rna levels of il-1β and toll-like recept ...201222884338
perivalvular pseudoaneurysm caused by streptococcus dysgalactiae in the presence of prosthetic aortic valve endocarditis.a 62-year-old man with a medical history of aortic valve replacement was referred to our hospital with high-grade fever. blood culture was positive for streptococcus dysgalactiae, and the echocardiogram showed edematous aortic annulus, suggesting a perivalvular abscess. treatment with antibiotics was started, which showed progressive improvement. the echocardiogram at 2 weeks after admission showed progression of the perivalvular abscess, resulting in the formation of a perivalvular pseudoaneruy ...201222791003
streptococcus dysgalactiae subspecies dysgalactiae infection after total knee arthroplasty: a case report.streptococcus dysgalactiae subspecies dysgalactiae (sdsd), lancefield group c streptococcus, is an animal pathogen which often causes pyogenic infection in domestic animals. human infection by sdsd has been reported as a cellulitis on the upper arm, but a prosthetic joint infection caused by sdsd after total knee arthroplasty (tka) has not yet been reported in the literature demonstrating that its clinical manifestation and management have not been well established. in this case report, we aimed ...201222708114
salivaricin g32, a homolog of the prototype streptococcus pyogenes nisin-like lantibiotic sa-ff22, produced by the commensal species streptococcus salivarius.salivaricin g32, a 2667 da novel member of the sa-ff22 cluster of lantibiotics, has been purified and characterized from streptococcus salivarius strain g32. the inhibitory peptide differs from the streptococcus pyogenes-produced sa-ff22 in the absence of lysine in position 2. the salivaricin g32 locus was widely distributed in blis-producing s. salivarius, with 6 (23%) of 26 strains pcr-positive for the structural gene, slna. as for most other lantibiotics produced by s. salivarius, the salivar ...201222567013
region specific and worldwide distribution of collagen-binding m proteins with parf motifs among human pathogenic streptococcal isolates.some of the variety of streptococcus pyogenes and streptococcus dysgalactiae ssp. equisimilis (sdse) m proteins act as collagen-binding adhesins that facilitate acute infection. moreover, their potential to trigger collagen autoimmunity has been implicated in the pathogenesis of acute rheumatic fever and attributed to a collagen-binding motif called parf (peptide associated with rheumatic fever). for the first time we determine the rate of clinical isolates with collagen-binding m proteins that ...201222253902
streptococcus dysgalactiae endocarditis presenting as acute endophthalmitis.endogenous endophthalmitis is a rare ocular infection affecting the vitreous and/or aqueous humours. it is associated with poor visual prognosis and its commonest endogenous aetiology is infective endocarditis. the causative organisms of endogenous endophthalmitis complicating endocarditis are mainly group a or b streptococci. the identification of group c and g streptococci such as streptococcus dysgalactiae is comparatively uncommon and has only been reported in a few case reports or series. w ...201224470923
molecular characterization of invasive streptococcus dysgalactiae subsp. equisimilis. multicenter study: argentina 2011-2012.streptococcus dysgalactiae subsp. equisimilis (sdse) has virulence factors similar to those of streptococcus pyogenes. therefore, it causes pharyngitis and severe infections indistinguishable from those caused by the classic pathogen. the objectives of this study were: to know the prevalence of sdse invasive infections in argentina, to study the genetic diversity, to determine the presence of virulence genes, to study antibiotic susceptibility and to detect antibiotic resistance genes. conventio ...201728341023
potential factors enabling human body colonization by animal streptococcus dysgalactiae subsp. equisimilis strains.streptococcus dysgalactiae subsp. equisimilis (sdse) is a pyogenic, lancefield c or g streptococcal pathogen. until recently, it has been considered as an exclusive animal pathogen. nowadays, it is responsible for both animal infections in wild animals, pets, and livestock and human infections often clinically similar to the ones caused by group a streptococcus (streptococcus pyogenes). the risk of zoonotic infection is the most significant in people having regular contact with animals, such as ...201728314902
infective endocarditis and infected aneurysm caused by streptococcus dysgalactiae subsp. equisimilis: a case report.endocarditis caused by streptococcus dysgalactiae subsp. equisimilis (sdse) is rare. infected aneurysm is one of the most serious complications of infective endocarditis. however, no reports have described sdse-related infected aneurysm. we herein report a successfully treated case of sdse-associated infective endocarditis with an infected aneurysm.201728174648
throat culture positivity rate and antibiotic susceptibility pattern of beta-hemolytic streptococci in children on secondary prophylaxis for rheumatic heart disease.among children diagnosed to have chronic rheumatic valvular heart disease (rhd) in ethiopia, many have been observed to develop recurrence of rheumatic fever (rf) despite secondary prophylaxis. this study determined the throat culture positivity rate and drug susceptibility pattern of beta hemolytic streptococci (bhs) isolated from children attending a specialized cardiac clinic in ethiopia.201627663649
description and characterization of a penicillin-resistant streptococcus dysgalactiae subsp. equisimilis clone isolated from blood in three epidemiologically linked patients.during a 27 month period, we detected four incidents of penicillin-resistant (pr) streptococcus dysgalactiae subsp. equisimilis (sdse) isolated from blood cultures of three patients.201627585966
beta-hemolytic streptococcus dysgalactiae strains isolated from horses are a genetically distinct population within the streptococcus dysgalactiae taxon.the pathogenic role of beta-hemolytic streptococcus dysgalactiae in the equine host is increasingly recognized. a collection of 108 lancefield group c (n = 96) or l (n = 12) horse isolates recovered in the united states and in three european countries presented multilocus sequence typing (mlst) alleles, sequence types and emm types (only 56% of the isolates could be emm typed) that were, with few exceptions, distinct from those previously found in human streptococcus dysgalactiae subsp. equisimi ...201627530432
streptococcus dysgalactiae subsp. equisimilis isolated from infections in dogs and humans: are current subspecies identification criteria accurate?streptococcus dysgalactiae is a pyogenic species pathogenic both for humans and animals. until recently, it has been considered an exclusive animal pathogen causing infections in wild as well as domestic animals. currently, human infections are being reported with increasing frequency, and their clinical picture is often similar to the ones caused by streptococcus pyogenes. due to the fact that s. dysgalactiae is a heterogeneous species, it was divided into two subspecies: s. dysgalactiae subsp. ...201627502064
recurrent sdse bacteraemia resulting in streptococcal toxic shock syndrome in a patient with noonan syndrome.a 19-year-old man with chronic lymphoedema due to noonan syndrome presented at our hospital with septic shock and pain in his lower leg. blood cultures were positive for streptococcus dysgalactiae subsp equisimilis (sdse), resulting in a diagnosis of cellulitis with toxic involvement. he was treated with ampicillin for 3 weeks. although he did well for 6 weeks, septic shock recurred. blood culture again revealed sdse, with the strain being identical to the first episode, suggesting that this inf ...201627485878
first molecular evidence of intrauterine and surgical-site infections caused by streptococcus dysgalactiae subsp. equisimilis.s. dysgalactiae subsp. equisimilis (sdse) is infrequently associated with maternal infections during delivery in pregnant women. a rare case is presented of a woman with intrauterine infection and surgical-site infection due to sdse after cesarean section, which had colonized her genital tract and, via the ascending pathway, reached her intact fetal membrane. all isolates were identified as streptococcus lancefield group g, and their emm genes that coded m protein belonged to stg6.1. the isolate ...201627367018
antimicrobial susceptibility and genetic relationships among streptococcus dysgalactiae subsp. equisimilis isolates in rio de janeiro.streptococcus dysgalactiae subsp. equisimilis (sdse) has been increasingly associated with several infectious diseases, ranging from pharyngitis to life-threatening conditions, such as necrotizing fasciitis and streptococcal toxic shock syndrome. however, its molecular epidemiology in some geographical areas remains unclear.201627301015
genetic diversity in streptococcus dysgalactiae subsp. equisimilis isolates from patients with invasive and noninvasive infections in a japanese university hospital (2014-2015).streptococcus dysgalactiae subsp. equisimilis (sdse) isolates with β-hemolysis and carbohydrate groups g or c are increasingly recovered from invasive infections in japan. the aim of this study was to determine the epidemiological characteristics of sdse isolates circulating locally among patients with invasive and noninvasive infections. we selected groups g/c β-hemolytic streptococci from a repository at the clinical laboratory of kitasato university medical center, from may 2014 through april ...201727000456
molecular characterization and antibiotic resistance of clinical streptococcus dysgalactiae subsp. equisimilis in beijing, china.streptococcus dysgalactiae subsp. equisimilis (sdse) is presently considered as a human pathogen associated with clinical infection. we characterized 56 sdse isolates collected from two tertiary hospitals in beijing, china. sixteen distinct emm types/subtypes were detected, dominated by stg245.0 (32.1%), stg652.0 (10.7%), stg6.1 (10.7%) and stg485.0 (10.7%), and a novel stg840.0 variant type was identified. all isolates possessed virulence genes of saga and scpa, and most carried slo (98.2%), sk ...201626925701
a highly susceptible cd46 transgenic mouse model of subcutaneous infection with streptococcus dysgalactiae subspecies equisimilis.the streptococcus dysgalactiae subspecies equisimilis (sdse) possesses clinical similarities to group a streptococcus (gas) and has recently been recognized as a causative pathogen of life-threatening streptococcal infections. human membrane cofactor protein (cd46), a complement regulatory protein ubiquitously expressed on every cell type except for erythrocytes, has been implicated as a receptor for human-specific pathogens including gas. in the present report, sdse strain ggs_124 was isolated ...201626908231
severe invasive streptococcal infection by streptococcus pyogenes and streptococcus dysgalactiae subsp. equisimilis.streptococcus pyogenes, a group a streptococcus (gas), has been recognized as the causative pathogen in patients with severe invasive streptococcal infection with or without necrotizing fasciitis. in recent epidemiological studies, streptococcus dysgalactiae subsp. equisimilis (sdse) has been isolated from severe invasive streptococcal infection. complete genome sequence showed that sdse is the closest bacterial species to gas, with approximately 70% of genome coverage. sdse, however, lacks seve ...201626762200
molecular characterization of invasive streptococcus dysgalactiae subsp. equisimilis, japan.we collected β-hemolytic streptococci (1,611 isolates) from patients with invasive streptococcal infections in japan during april 2010-march 2013. streptococcus dysgalactiae subsp. equisimilis (sdse) was most common (n = 693); 99% of patients with sdse infections were elderly (mean age 75 years, sd ±15 years). we aimed to clarify molecular and epidemiologic characteristics of sdse isolates and features of patient infections. bacteremia with no identified focus of origin and cellulitis were the m ...201626760778
increased cytotoxicity and streptolysin o activity in group g streptococcal strains causing invasive tissue infections.streptococcus dysgalactiae subsp. equisimilis (sdse) has emerged as an important cause of severe skin and soft tissue infections, but little is known of the pathogenic mechanisms underlying tissue pathology. patient samples and a collection of invasive and non-invasive group g sdse strains (n = 69) were analyzed with respect to virulence factor expression and cytotoxic or inflammatory effects on human cells and 3d skin tissue models. sdse strains efficiently infected the 3d-skin model and severe ...201526601609
assessment and characterization of biofilm formation among human isolates of streptococcus dysgalactiae subsp. equisimilis.the capacity to form biofilm is considered a protective mechanism that allows the bacteria to survive and proliferate in hostile environments, facilitating the maintenance of the infectious process. recently, biofilm has become a topic of interest in the study of the human pathogen group a streptococcus (gas). although gas has not been associated with infection on medical implants, the presence of microcolonies embedded in an extracellular matrix on infected tissues has been reported. despite th ...201526558847
prevalence of emm types and antimicrobial susceptibility of streptococcus dysgalactiae subsp. equisimilis in austria.an increase of severe infections caused by streptococcus dysgalactiae subsp. equisimilis (sdse) similar to infections caused by streptococcus pyogenes has been reported over the last years. little is known about infections with sdse in austria. therefore, we investigated a collection of 113 sdse invasive and non-invasive isolates from different infection sites and type of infections as well as patients' characteristics.201526507866
draft genome sequences of four genetically distinct human isolates of streptococcus dysgalactiae subsp. equisimilis.β-hemolytic group c and group g streptococci (gcs-ggs; streptococcus dysgalactiae subsp. equisimilis) emerged as human pathogens in the late 1970s. we report here the draft genome sequences of four genetically distinct human strains of gcs-ggs isolated between the 1960s and 1980s. comparative analysis of these genomes may provide a deeper understanding of gcs-ggs genome and virulence evolution.201526430051
a rare case of spontaneous acute bacterial arthritis of the glenohumeral joint attributed to streptococcus dysgalactiae subsp. equisimilis. 201726288541
seropositivity for antibodies to drs-g, a virulence factor from streptococcus dysgalactiae subsp. equisimilis, is an independent risk factor for poststreptococcus glomerulonephritis and chronic kidney disease in mumbai, india.the disease spectrum caused by streptococcus dysgalactiae subsp. equisimilis resembles that of s. pyogenes (group a streptococcus [gas]). these two bacterial species are closely related and possess many common virulence characteristics. while some gas strains express virulence factors called streptococcal inhibitor of complement (sic) and distantly related to sic (drs), some s. dysgalactiae subsp. equisimilis isolates express an orthologue of drs, which is referred to as drs-g. we reported previ ...201526084513
small plasmids in streptococcus dysgalactiae subsp. equisimilis isolated from human infections in southern india and sequence analysis of two novel plasmids.small plasmids are frequently found in s. pyogenes isolates from human infections in india. streptococcus dysgalactiae subsp. equisimilis (sdse) is a streptococcal subspecies that is genetically similar to s. pyogenes and has a similar ecology. therefore, we determined the distribution of small plasmids in a collection of 254 sdse isolates, comprising 44 different emm-types and emm non-typable strains, from southern india, utilizing an established pcr based method. briefly, 1.2% (n=3) of the iso ...201525769407
group c streptococcus dysgalactiae subsp. equisimilis in south-east brazil: genetic diversity, resistance profile and the first report of human and equine isolates belonging to the same multilocus sequence typing lineage.streptococcus dysgalactiae subsp. equisimilis (sdse) isolates are the most common group c streptococci in humans and reports of invasive infections associated with sdse have been increasing. molecular epidemiology studies are an important strategy to trace the emergence and spread of possible well-fit bacterial pathogens of humans and animals. in this work, we analysed the antimicrobial and clonal profiles of 115 sdse infection and colonization isolates of human and equine origin. pfge revealed ...201525752850
prevalence of streptococcus dysgalactiae subsp. equisimilis and s. equi subsp. zooepidemicus in a sample of healthy dogs, cats and horses.to estimate the prevalence of β-haemolytic lancefield group c streptococci in healthy dogs, cats and horses; to determine if frequent contact with horses was associated with isolation of these species from dogs and cats; and to characterise recovered s. equi subsp. zooepidemicus isolates by multilocus sequence typing.201525695401
horizontal gene transfer and recombination in streptococcus dysgalactiae subsp. equisimilis.streptococcus dysgalactiae subsp. equisimilis (sdse) is a human pathogen that colonizes the skin or throat, and causes a range of diseases from relatively benign pharyngitis to potentially fatal invasive diseases. while not as virulent as the close relative streptococcus pyogenes the two share a number of virulence factors and are known to coexist in a human host. both pre- and post-genomic studies have revealed that horizontal gene transfer (hgt) and recombination occurs between these two organ ...201425566202
streptokinase production from streptococcus dysgalactiae subsp. equisimilis sk-6 in the presence of surfactants, growth factors and trace elements.streptokinase is an extracellular protein secreted by various strains of streptococci and is used clinically as an intravenous thrombolytic agent for the treatment of acute myocardial infarction. it is well established that the fibrinolytic activity of streptokinase originates in its ability to activate plasminogen. the present investigation was carried out to determine the extent of streptokinase production by streptococcus dysgalactiae subsp. equisimilis sk-6, in the presence of surfactants, g ...201528324574
streptococcal endocarditis in a captive southern white rhinoceros (ceratotherium simum simum).postmortem examination of a 43-yr-old male southern white rhinoceros (ceratotherium simum simum) revealed gross lesions and histopathologic findings consistent with endocarditis. the animal was born in umfolozi national park, south africa, and then it was moved at 2 yr of age to two successive european zoologic collections. for several weeks prior to death, the animal was increasingly recumbent or assuming a dog-sitting position. postmortem examination revealed cutaneous pressure sores and multi ...201425314832
[spondylodiscitis by streptococcus dysgalactiae subsp. equisimilis (group g)]. 201424940896
pcr based differentiation between streptococcus dysgalactiae subsp. equisimilis strains isolated from humans and horses.streptococcus dysgalactiae subsp. equisimilis (sdse) can be severely pathogenic in humans and is increasingly isolated from horses with respiratory, reproductive or other diseases, although it is often considered a commensal bacterium. here a pcr protocol is described for identifying sdse recovered from humans. a multiplex pcr targeting the 16s rrna and the streptokinase precursor gene has been optimized for differentiating between sdse strains isolated from humans and those isolated from horses ...201424813401
streptococcus dysgalactiae subsp. equisimilis bacteremia: an emerging infection.the importance of group c and g streptococcus dysgalactiae subspecies equisimilis (s. dysgalactiae subsp. equisimilis) as a significant pathogen has recently been better recognized. s. dysgalactiae subsp. equisimilis disease can range in severity from milder skin and soft-tissue conditions such as wound infection, erysipelas, and cellulitis, to life-threatening necrotizing fasciitis and streptococcal toxic shock syndrome, thus sharing the clinical picture with s. pyogenes. the most common clinic ...201424682845
two cases of cardiac device-related endocarditis due to streptococcus dysgalactiae subsp. equisimilis (group c or g streptococci).cardiac device-related endocarditis is a very rare clinical manifestation of s. dysgalactiae subsp. equisimilis disease. this pathogen is a common cause of cellulitis. we here report two cases of cardiac device-related endocarditis due to streptococcus dysgalactiae subsp. equisimilis. blood cultures yielded this pathogen and both patients had recurrent bacteremia. transthoracic and transesophageal echocardiography revealed lead vegetations. this is a new description of this pathogen to cause car ...201424678588
drsg from streptococcus dysgalactiae subsp. equisimilis inhibits the antimicrobial peptide ll-37.sic and drs are related proteins present in only 4 of the >200 streptococcus pyogenes emm types. these proteins inhibit complement-mediated lysis and/or the activity of certain antimicrobial peptides (amps). a gene encoding a homologue of these proteins, herein called drsg, has been identified in the related bacterium streptococcus dysgalactiae subsp. equisimilis. here we show that geographically dispersed isolates representing 14 of 50 emm types examined possess variants of drsg. however, not a ...201424664506
correlation of virulence genes to clinical manifestations and outcome in patients with streptococcus dysgalactiae subspecies equisimilis bacteremia.streptococcus dysgalactiae subsp. equisimilis (sdse) is increasingly recognized as a human pathogen responsible for invasive infection and streptococcal toxic shock syndrome (stss). the pathogen possesses virulence genes that resemble those found in streptococcus pyogenes (gas). we analyzed the association between these specific toxic genes, clinical presentations, and outcome in patients with sdse infections.201424210866
complete genome sequence of streptococcus dysgalactiae subsp. equisimilis 167 carrying lancefield group c antigen and comparative genomics of s. dysgalactiae subsp. equisimilis strains.streptococcus dysgalactiae subsp. equisimilis (sdse) is an emerging human pathogen that causes life-threatening invasive infections such as streptococcal toxic shock syndrome. recent epidemiological studies reveal that invasive sdse infections have been increasing in asia, europe, and the united states. almost all sdse carry lancefield group g or c antigen. we have determined the complete genome sequence of a human group c sdse 167 strain. a comparison of its sequence with that of four sdse stra ...201323918808
concomitant regulation of host tissue-destroying virulence factors and carbohydrate metabolism during invasive diseases induced by group g streptococci.streptococcus dysgalactiae subsp. equisimilis (sdse) has lancefield group g or c antigens. recent epidemiological studies reveal that invasive sdse infections have been increasing in asia, europe, and the united states. the mechanisms and key virulence factors by which sdse causes invasive diseases are poorly understood.201323901096
interspecies communication among commensal and pathogenic streptococci.quorum sensing (qs) regulates diverse and coordinated behaviors in bacteria, including the production of virulence factors, biofilm formation, sporulation, and competence development. it is now established that some streptococci utilize rgg-type proteins in concert with short hydrophobic peptides (shps) to mediate qs, and sequence analysis reveals that several streptococcal species contain highly homologous rgg/shp pairs. in group a streptococcus (gas), two shps (shp2 and shp3 [shp2/3]) were pre ...201323882015
necrotizing soft tissue infections caused by streptococcus pyogenes and streptococcus dysgalactiae subsp. equisimilis of groups c and g in western norway.streptococcus pyogenes (group a streptococcus, gas) is a major cause of necrotizing soft tissue infection (nsti). on rare occasions, other β-haemolytic streptococci may also cause nsti, but the significance and nature of these infections has not been thoroughly investigated. in this study, clinical and molecular characteristics of nsti caused by gas and β-haemolytic streptococcus dysgalactiae subsp. equisimilis of groups c and g (gcs/ggs) in western norway during 2000-09 are presented. clinical ...201323795951
transmission of streptococcus dysgalactiae subsp. equisimilis between child and dog in an aboriginal australian community.streptococcus dysgalactiae subsp. equisimilis (sdse), also known as group g and c streptococci, is becoming increasingly recognized as a pathogen in humans. we report here the finding of an identical strain of sdse in the throat of a child and their dog in an aboriginal australian community. the strain was identified using the api 20strep system, 16s rrna gene sequencing, emm sequence typing (emmst) and multilocus sequence typing (mlst) as a group c sdse, stc839.5 and st-3. carriage of this stra ...201423745768
adult invasive and noninvasive infections due to streptococcus dysgalactiae subsp. equisimilis in france from 2006 to 2010.we characterized 182 streptococcus dysgalactiae subsp. equisimilis isolates and analyzed the epidemiological data on the corresponding infections. stg6, stg485, and stg6792 were the 3 most prevalent invasive emm types among the 27 different emm types recovered. high rates of antimicrobial resistance were observed for macrolides (26.4%) and tetracycline (34.6%).201323698531
a fatal case of streptococcal toxic shock syndrome due to streptococcus dysgalactiae subsp. equisimilis possibly caused by an intramuscular injection.an 88-year-old man died of streptococcal toxic shock syndrome due to a group g streptococcus infection that was possibly caused by an intramuscular injection given 30 hours earlier in his right deltoid muscle. the causative pathogen was later identified to be streptococcus dysgalactiae subsp. equisimilis (stg485). although providing intramuscular injections is an essential skill of health care workers that is performed daily worldwide, it may constitute a port of entry for pathogens via skin bre ...201323370754
lancefield group g streptococcus dysgalactiae subsp. equisimilis: an unusual aetiology of perianal streptococcal dermatitis acquired from heterosexual oral-anal intercourse.perianal streptococcal dermatitis (psd) is an uncommon superficial cutaneous infection of the perianal area, almost exclusively described in children and mainly caused by group a streptococci. we report here a case of psd caused by streptococcus dysgalactiae subsp. equisimilis, lancefield group g, in an adult man due to heterosexual oral-anal sexual contact.201223258838
a case of streptococcal toxic shock syndrome due to group g streptococci identified as streptococcus dysgalactiae subsp. equisimilis.a 79-year-old man with a 3-month history of lymphedema of the lower limbs, and diabetes mellitus, was admitted to our hospital for suspected deep venous thrombosis. several hours after admission, leg pain and purpura-like skin color appeared. on the 2nd hospital day, he was referred to our department for possible acute occlusive peripheral artery disease (pad) and skin necrosis with blisters; however, computed tomography with contrast showed no occlusive lesions. he had already developed shock a ...201222327489
validity of somatic cell count as indicator of pathogen-specific intramammary infections.the objective of this study was to determine whether somatic cell count (scc) was an effective test, with a sensitivity exceeding 85%, to determine species-specific bacterial infections. in addition, the relation between the scc and various udder pathogen groups was investigated. scc thresholds of greater than 200 000 cells/ml were used in quarter and greater than 150 000 cells/ml in composite milk samples. a retrospective study was conducted on a data set for 89 635 quarter and 345 467 composit ...201728470079
epidemiological study of common diseases and their risk factors in camels in south punjab, pakistan.bacteriological study of mastitis along with common blood protozoan diseases were studied in dromedary camels in cholistan, dera ismail khan and rahim yar khan districts in south punjab, pakistan. for this purpose 300 camels were sampled randomly at different common grazing and watering point. for study of blood parasites clinically suspected and apparently healthy camels, 150 each, were sampled. an overall prevalence of 15%and 5% was recorded for trypanosomiasis and anaplasmosis respectively. t ...201728428133
bacteriological etiology and treatment of mastitis in finnish dairy herds.the finnish dairy herd recording system maintains production and health records of cows and herds. veterinarians and farmers register veterinary treatments in the system. milk samples for microbiological analysis are routinely taken from mastitic cows. the laboratory of the largest dairy company in finland, valio ltd., analyzes most samples using real-time pcr. this study addressed pathogen-specific microbiological data and treatment and culling records, in combination with cow and herd characte ...201728545485
in vitro synergistic activities of cefazolin and nisin a against mastitis pathogens.first-generation cephalosporins such as cefazolin (cez) have been widely used for mastitis treatment in dairy cattle. however, the use of antibiotics results in the presence of antibiotic residues in milk, which is used for human consumption. nisin a, a bacteriocin produced by lactococcus lactis, has been used as a broad-spectrum food preservative for over 50 years. therefore, a combination of cez and nisin a might provide an extended activity spectrum against mastitis pathogens and reduce the a ...201728757508
bovine intra-mammary challenge with streptococcus dysgalactiae spp. dysgalactiae to explore the effect on the response of complement activity.recently published work as described by the authors highlighted the extent of complement activity in bovine milk. localised mastitis infection occurring in the mammary glands of dairy cows is readily detectable by the levels of somatic cells in milk. thus, it is opportune to monitor complement activity in milks in association with the animal's innate immune response to mammary infection. preliminary screening of milk samples taken randomly showed that milk with a high somatic cell count (scc) re ...201728625182
incidence of clinical mastitis and distribution of pathogens on large chinese dairy farms.knowledge of the incidence of clinical mastitis (cm) and the distribution of pathogens involved is essential for development of prevention and control programs as well as treatment protocols. no country-wide study on the incidence of cm and the distribution of pathogens involved has been conducted in china. core objectives of this study were, therefore, to determine the cumulative incidence of cm and the distribution of pathogens causing cm on large chinese (>500 cows) dairy farms. in addition, ...201728434736
an ecological approach to discover new bioactive extracts and products: the case of extremophile plants.eight extremophile plants from tunisia were screened to find natural products with benefits in human health.201728444868
use of maldi-tof mass spectrometry for the fast identification of gram-positive fish pathogens.gram-positive cocci, such as streptococcus agalactiae, lactococcus garvieae, streptococcus iniae, and streptococcus dysgalactiae subsp. dysgalactiae, are found throughout the world, particularly in outbreaks in farmed fish, and are thus associated with high economic losses, especially in the cultivation of nile tilapia. the aim of this study was to evaluate the efficacy of matrix-assisted laser desorption ionization (maldi)-time of flight (tof) mass spectrometry (ms) as an alternative for the di ...201728848512
within-herd prevalence thresholds for herd-level detection of mastitis pathogens using multiplex real-time pcr in bulk tank milk samples.the objective of the study was to assess the value of quantitative multiplex real-time pcr examination of bulk tank milk samples for bovine mastitis pathogens as a tool for herd level diagnosis. using a logistic regression model, this study is aimed at calculating the threshold level of the apparent within-herd prevalence as determined by quarter milk sample cultivation of all lactating cows, thus allowing the detection of a herd positive for a specific pathogen within certain probability levels ...201728780090
development of a multiplex pcr for identification of β-hemolytic streptococci relevant to human infections and serotype distribution of invasive streptococcus agalactiae in thailand.a multiplex polymerase chain reaction (mpcr) was developed for simultaneous detection (single reaction) of genes specific to five frequent clinically relevant β-hemolytic streptococcal species: streptococcus pyogenes (spy1258), streptococcus agalactiae (cfb and cpn60), streptococcus dysgalactiae subsp. equisimilis (16s-23s intergenic spacer) , s. equi subsp. zooepidemicus (esaa and sord), and streptococcus anginosus group (moac). no cross-reaction was observed with other bacterial species. this ...201728666619
phenotypic and genotypic antimicrobial susceptibility pattern of streptococcus spp. isolated from cases of clinical mastitis in dairy cattle in poland.mastitis of dairy cattle is one of the most frequently diagnosed diseases worldwide. the main etiological agents of mastitis are bacteria of the genus streptococcus spp., in which several antibiotic resistance mechanisms have been identified. however, detailed studies addressing this problem have not been conducted in northeastern poland. therefore, the aim of our study was to analyze, on phenotypic and genotypic levels, the antibiotic resistance pattern of streptococcus spp. isolated from clini ...201728601447
cutaneous eruption associated with streptococcus dysgalactiae (group c streptococcus) bacteremia. 201728831413
emergence of a streptococcus dysgalactiae subspecies equisimilis stg62647-lineage associated with severe clinical manifestations.increasing incidence rates of invasive streptococcus dysgalactiae subspecies equisimilis (sdse) infections have been reported worldwide, but the evolutionary mechanisms underlying this development remain elusive. through prospective surveillance of invasive sdse infections in western norway, we observed the emergence of a novel and virulent sdse genotype, stg62647. this emm-type, rarely encountered as a cause of invasive disease during 1999-2012, emerged in 2013 as the predominant sdse-genotype. ...201728790435
clinical and molecular characteristics of infective β-hemolytic streptococcal endocarditis.streptococcus pyogenes (s. pyogenes) and streptococcus dysgalactiae subspecies equisimilis (sdse) cause considerable morbidity and mortality, and show similarities in disease manifestations and pathogenic mechanisms. their involvement in infective endocarditis, however, has not been well described. invasive s. pyogenes and sdse infections in health region bergen, norway, in the period 1999-2013 were reviewed, and sixteen cases of endocarditis were identified. the median duration of symptoms was ...201728780243
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