infection due to staphylococcus warneri. | | 1992 | 1360838 |
effect of various microbial preparations on p-388 mouse lymphocytic leukemia. | four bacteria-derived immunopotentiators were tested for their protective effect on a p-388 mouse lymphocytic leukemia model. the microbial test products were prepared from the following bacterial strains: atcc 35983 staphylococcus epidermidis isolated from a patient with iv catheter; atcc 31874, a patented strain listed as staphylococcus epidermidis isolated from the urine of a cancer patient; atcc 25615 staphylococcus hominis obtained from a child with lymphocytic leukemia, and atcc 25614 stap ... | 1992 | 1475343 |
significant infection caused by staphylococcus warneri. | | 1992 | 1500540 |
clinical significance of staphylococcus warneri bacteremia. | twenty-seven episodes of bacteremia caused by staphylococcus warneri were identified at long island jewish medical center in new york between 1984 and 1989. fourteen of these were thought to represent true bacteremias and 13 to represent contaminants. of the 14 true bacteremias, 5 were in pediatric and 9 were in adult patients. eight of 14 patients (57%) had catheter-related bacteremia and 5 of 14 had bacteremia of unknown source. there was one case of fulminant native valve s. warneri endocardi ... | 1992 | 1537891 |
ventriculoatrial shunt infection caused by staphylococcus warneri: case report and review. | staphylococcus warneri, a coagulase-negative species, is a rare cause of infection of cerebrospinal fluid (csf) shunts. in one recently studied case of ventriculoatrial shunt infection, the repeated isolation of s. warneri (i.e., from all of six blood cultures and from a csf sample obtained directly from the valve of the shunt) suggested that this organism can be clinically significant. review of the literature clearly indicates that s. warneri is a rare but potentially dangerous pathogen in bot ... | 1992 | 1571462 |
swai, a unique restriction endonuclease from staphylococcus warneri, which recognizes 5'-atttaaat-3'. | a novel class-ii restriction endonuclease designated swai was purified from staphylococcus warneri. this enzyme cleaves adenovirus 2 dna, sv40 dna and m13mp7 at one site each, but does not cleave lambda, phix174, pbr322 or pbr328 dna. swai recognizes the octanucleotide sequence 5'-atttaaat-3', cleaving in the center of the recognition sequence creating blunt ended dna fragments. swai was used to digest chromosomal dna from various microorganisms and human cells. | 1992 | 1594448 |
[haematogenous osteomyelitis induced by strain smu-0611 of staphylococcus warneri in mice]. | strain smu-0611 of staphylococcus warneri isolated from clinical specimens (1.25 x 10(9) colony forming units) was intravenously administered into mice. strain smu-0611 caused remarkable inflammation characterized by roentgenographically recognizable changes in bone at 14 days after the injection. a number of organisms were detected in the bone marrow for as long as 35 days after the injection, suggesting specific lodgement of the strain in mouse bone marrow. concerning the x-ray features of the ... | 1991 | 1761911 |
the occurrence and growth of microorganisms during the fermentation of fish sausage. | minced fish (mullet) sausage mixes containing added sugar, salt, nitrate, nitrite and spices were fermented (48 h, 30 degrees c) by indigenous flora or by a starter culture (pediococcus acidilactici) and the microbial ecology and behaviour of various bacteria was monitored. pediococcus pentosaceus and lactobacillus plantarum dominated the indigenous fermentation, achieving populations of 10(7)-10(8) cfu/g by 48 h, and decreasing the ph of the mix to 4.5-4.7. significant growth (10(5)-10(7) cfu/g ... | 1991 | 1909546 |
evaluation of the staph-zym system with staphylococci isolated from bovine intramammary infections. | a total of 148 staphylococci isolated from bovine intramammary infections were used to evaluate the staph-zym system (rosco, taastrup, denmark). the overall accuracy of the system was 91.9%. the system correctly identified all strains of staphylococcus aureus, staphylococcus simulans, and staphylococcus xylosus and 95% of staphylococcus intermedius strains. of 33 staphylococcus hyicus strains, 31 (93.9%) were classified correctly by the staph-zym system, as well as 8 (80%) of 10 staphylococcus c ... | 1991 | 1993769 |
a convenient method for differentiation of coagulase-negative staphylococci isolated from bovine mammary glands. | the utility of trehalose-mannitol broth and arabinose-cellobiose broth for identification of staphylococcus epidermidis and novobiocin-resistant staphylococci was determined using 236 coagulase-negative staphylococci isolated from bovine mammary glands. none of the 49 s. epidermidis strains was positive in trehalose-mannitol broth; whereas, all strains of staphylococcus hyicus, staphylococcus chromogenes, staphylococcus haemolyticus, staphylococcus hominis, staphylococcus warneri, and staphyloco ... | 1991 | 2045549 |
typing of coagulase-negative staphylococci by southern hybridization of chromosomal dna fingerprints using a ribosomal rna probe. | ribotyping consists of restriction endonuclease fingerprinting of ribosomal rna (rrna) genes visualized by southern hybridization with an rrna probe. this method was developed and compared with restriction endonuclease fingerprinting of chromosomal dna for typing coagulase-negative staphylococci. twenty-five american type culture collection reference type strains and 53 clinical isolates were typed. both methods clearly distinguished all 15 species of coagulase-negative staphylococci and most in ... | 1990 | 2209626 |
pathogenicity of staphylococcus lugdunensis, staphylococcus schleiferi, and three other coagulase-negative staphylococci in a mouse model and possible virulence factors. | staphylococcus lugdunesis and staphylococcus schleiferi, two newly described species, have been isolated from numerous types of human infections. we compared the pathogenicity of 30 strains of s. lugdunensis, s. schleiferi, staphylococcus epidermidis, staphylococcus warneri, and staphylococcus hominis, using a mouse model in which a foreign body preadhered with the test strain was implanted subcutaneously, followed by injection of the test strain. all five species of staphylococci produced absce ... | 1990 | 2224644 |
fluorogenic assay for differentiating staphylococcus warneri and staphylococcus hominis strains of bovine origin. | a fluorogenic assay for the detection of beta-glucosidase was developed as part of a simplified conventional method to distinguish staphylococcus warneri and staphylococcus hominis isolated from bovine body sites. the assay is based on the fact that strains of s. warneri produce beta-glucosidase, while strains of s. hominis do not. | 1990 | 2324280 |
studies on antagonism between porcine skin bacteria. | strains of staphylococcus warneri, staph. epidermidis and aerococcus viridans isolated from porcine skin were shown to produce substances that inhibited the growth of staph. hyicus. studies of interactions between the inhibitor-producing bacteria and staph. hyicus on cellulose acetate membranes demonstrated a significant reduction (p less than 0.001) of the staph. hyicus population at various stages of its growth cycle, depending on the inhibitor used. inhibitor production profiles in batch cult ... | 1989 | 2666380 |
vertebral osteomyelitis and native valve endocarditis caused by staphylococcus warneri. | coagulase-negative staphylococci almost invariably cause significant clinical infections in the setting of prosthetic devices or severely compromised host defenses. hematogenous osteomyelitis and native valve endocarditis due to these pathogens in any setting is rare. we report a case of community-acquired vertebral osteomyelitis and native valve endocarditis due to staphylococcus warneri in a patient with none of the usual risk factors. | 1989 | 2791489 |
recognition of staphylococcus saprophyticus in urine cultures by screening colonies for production of phosphatase. | phenolphthalein diphosphate was incorporated into a primary blood agar medium for use in performing quantitative urine cultures. phosphatase-negative staphylococci, such as staphylococcus saprophyticus, were differentiated from phosphatase-positive species, such as staphylococcus epidermidis, by spot testing colonies on filter paper saturated with 1 n naoh. phosphatase-positive colonies turned pink within seconds, and phosphatase-negative colonies showed no color. none of 55 s. saprophyticus iso ... | 1985 | 2984240 |
bactericidal mechanisms of ofloxacin. | ciprofloxacin and ofloxacin are known to exert a second bactericidal mechanism (termed b) against escherichia coli which functions even when protein synthesis is inhibited by chloramphenicol or when rna synthesis is inhibited by rifampicin. however, the bactericidal activity of ciprofloxacin against a coagulase-negative staphylococcus (staphylococcus warneri) was found to be abolished by chloramphenicol so the 4-quinolone does not exert mechanism b against this species. on the other hand, ofloxa ... | 1988 | 3053574 |
biofilms on right heart flow-directed catheters. | this study was designed to detect biofilm and bacteria on right heart flow-directed catheters using scanning electron microscopy and culture following scraping and dispersion of biofilm by sonication. we examined 20 consecutive catheters removed from 18 critically ill patients, an average of 2.6 days after insertion. on scanning electron microscopy, all catheters were found to be covered by a biofilm, with bacteria visible on 50 percent of them. cultures of specimens from 40 percent of the cathe ... | 1987 | 3304847 |
vertebral osteomyelitis due to staphylococcus warneri attributed to a hickman catheter. | a patient with a long-term right atrial (hickman) catheter developed vertebral osteomyelitis due to staphylococcus warneri. documentation of this event--to our knowledge previously unreported--was made possible by use of special studies including plasmid profiles of the coagulase-negative staphylococcal isolates. | 1987 | 3440368 |
reliability of latex agglutination tests for identification of staphylococcus aureus resistant to oxacillin. | commercial latex agglutination tests (lats) for the simultaneous detection of clumping factor and protein a are gaining increased acceptance as a means of identifying staphylococcus aureus. we evaluated two lats (accu-staph; carr-scarborough, decatur, ga.; staphaurex; wellcome, dartford, england) with particular emphasis on their ability to correctly identify oxacillin-resistant s. aureus. we tested 59 oxacillin-resistant s. aureus, 136 oxacillin-susceptible s. aureus, and 92 coagulase-negative ... | 1986 | 3640773 |
synergistic hemolysis associated with coagulase-negative staphylococci isolated from bovine mammary glands. | a total of 353 coagulase-negative staphylococcus (cns) isolates from infected bovine mammary glands were tested for cytolysin production by using the synergistic hemolysin assay (sha). overall, 34.6% of cns isolates were sha positive. human-associated, coagulase-negative staphylococcal species contained the greatest number of sha-positive strains. milk leukocyte levels expressed as somatic cell counts (scc) were elevated with sha-positive staphylococcus epidermidis, staphylococcus hominis, and s ... | 1987 | 3693535 |
vertebral osteomyelitis due to staphylococcus warneri. | we report a case of vertebral osteomyelitis in a diabetic woman. this appears to be the first report of such an infection with the coagulase negative staphylococcus, s warneri. | 1986 | 3813666 |
binding of human serum high density lipoprotein to staphylococcus capitis. | of twelve different staphylococcal species tested, staphylococcus capitis and staphylococcus warneri bound human serum high density lipoprotein (hdl). the binding of hdl was observed with bacteria cultivated of brain-heart infusion broth agar, but not on nutrient broth agar. the factor mediating the binding of hdl seems to be a bacterial component and not a component of the growth medium. studies on the binding of hdl to s. capitis showed that a subfraction of hdl was involved in the reaction. t ... | 1985 | 3936335 |
identification of coagulase-negative staphylococci isolated from urinary tract infections. | coagulase-negative staphylococci isolated from urinary tract infections were identified using the api staph-ident system. organisms were excluded if there was no sign of pyuria or if normal urethral flora was present in significant amounts. while staphylococcus saprophyticus and staphylococcus epidermidis accounted for 81% of the isolates from females, 87% of isolates from males were s. epidermidis, staphylococcus warneri, or staphylococcus haemolyticus. the females fell into two main age groups ... | 1986 | 3940428 |
clinically silent polymicrobial amnionitis and intrauterine fetal death associated with a cu-7 intrauterine contraceptive device. | this article presents a case of silent polymicrobial amnionitis with subsequent intrauterine fetal death in a 34-year old woman who conceived with a cu-7 iud in place. there were no apparent pregnancy complications or symptoms of uterine infection during early pregnancy. at 16 weeks gestation, the patient underwent amniocentesis for cytogenetic studies. 5 different microorganisms--corynebacterium, staphylococcus warneri, staphylococcus epidermidis, streptococcus mitis, and ureaplasma urealyti ... | 1984 | 6507538 |
identification, clinical distribution, and susceptibility to methicillin and 18 additional antibiotics of clinical staphylococcus isolates: nationwide investigation in italy. | a multicentric study of clinical staphylococcus isolates was performed by seven operative units working in different areas of italy. over a 6-month period, a total of 3,226 staphylococci, isolated from in- and outpatients, were identified and tested for antimicrobial susceptibility by a protocol agreed upon by all units. on the basis of their bacteriolytic-activity patterns and other conventional tests, the isolates were identified by lyogroups , which closely correlate with human staphylococcus ... | 1984 | 6565709 |
endocarditis caused by staphylococcus warneri on a normal aortic valve following vasectomy. | endocarditis caused by staphylococcus warneri and necessitating valve replacement occurred in a previously healthy 32-year-old patient following vasectomy. no sign of an underlying valvular defect was noted during the operation. s. warneri is a recently identified species of coagulase-negative staphylococci. endocarditis caused by coagulase-negative staphylococci is uncommon in young, healthy patients with normal heart valves and has not previously been described as a complication of vasectomy. ... | 1984 | 6744166 |
classification and characteristics of coagulase-negative, methicillin-resistant staphylococci. | sixty-five clinical isolates of coagulase-negative, methicillin-resistant staphylococci have been classified as staphylococcus epidermidis (63.0%), "phosphatase-negative s. epidermidis" (12.3%), coagulase-negative staphylococcus aureus (6.2%), staphylococcus hemolyticus (6.2%), staphylococcus hominis (3.1%), and staphylococcus warneri (1.5%). five of the organisms (7.7%) could not be classified with certainty as currently recognized species. novobiocin resistance was encountered in eight of the ... | 1980 | 6971871 |
speciation and antibiotic susceptibility patterns of coagulase-negative staphylococci. | during a six month period, 191 isolates of coagulase-negative staphylococci from blood, urine, cerebrospinal fluid and heart valves were identified to species level and tested for antimicrobial susceptibility. seventy-one percent of isolates were staphylococcus epidermidis, 8% staphylococcus warneri, 7% staphylococcus hominis, 7% staphylococcus haemolyticus, 4% staphylococcus capitis, 2% staphylococcus saprophyticus and 1% staphylococcus cohnii. approximately 4% of isolates were felt to be assoc ... | 1982 | 7173185 |
four-year prospective study of staph-ident system and conventional method for reference identification of staphylococcus, stomatococcus, and micrococcus spp.. | a 4-year prospective study compared the accuracy of the staph-ident system (biomérieux vitek, inc., hazelwood, mo.) with that of the reference procedure of the centers for disease control and prevention for the identification of staphylococcus species, stomatococcus mucilaginosus, and micrococcus species. the study compared the results from 1,106 cultures (500 eye cultures, 217 strains submitted for reference identification, and 389 known stock strains) representing 21 species of the family micr ... | 1995 | 7699074 |
purification and characterization of extracellular staphylococcus warneri lipase. | the extracellular lipase of staphylococcus warneri was secreted as a protein with an apparent molecular mass of 90 kda. it was then sequentially processed in the supernatant to a protein of 45 kda. tryptic digestion of the crude extract resulted in a homogeneous sample containing only the 45-kda form. purification was achieved by hydrophobic chromatography. purified lipase had an optimum ph of 9.0 and an optimum temperature of 25 degrees c. the enzyme was stable within the range ph 5.0-9.0; it h ... | 1995 | 7765877 |
posttraumatic meningitis: bacteriology, hydrocephalus, and outcome. | to investigate the conditions that have developed in the treatment of posttraumatic meningitis with the use of new antibiotics, the authors studied cases with this infection retrospectively for a period of 68 months. among 860 patients with moderate to severe head injuries, 12 (1.39%) sustained this complication. of these, nine patients (75%) had a demonstrable basilar skull fracture and seven (58.3%) presented obvious rhinorrhea. of these seven, four (57.1%) were treated conservatively and thre ... | 1994 | 7800133 |
pulsed-field gel electrophoresis of the genomic restriction fragments of coagulase-negative staphylococci. | the genomes of 47 coagulase-negative staphylococcal strains assigned to different species were analysed by pulsed-field electrophoresis. the strains were clustered on the basis of their similarity in the smai restriction patterns into various groups, each group consisting of the type strain and the strains whose smai restriction patterns were similar to that of the type of strain. the smai restriction groups seem to correspond to the following species: staphylococcus warneri, s. hominis, s. xylo ... | 1994 | 7813882 |
relatedness of coagulase-negative staphylococci causing bacteremia in low-birthweight infants. | to investigate coagulase-negative staphylococcus (cons) causing bacteremia in a neonatal intensive care unit (nicu). | 1994 | 7844337 |
distribution of coagulase-negative staphylococci, including the newly described species staphylococcus schleiferi, in nosocomial and community acquired urinary tract infections. | four hundred and four coagulase-negative staphylococci were isolated from 4905 urine specimens obtained from 4192 inpatients and outpatients. the distribution of the strains was as follows: 193 staphylococcus epidermidis (47.8%), 171 staphylococcus saprophyticus (42.3%), 29 staphylococcus haemolyticus (7.2%), 5 staphylococcus warneri (1.2%), 3 staphylococcus schleiferi (0.7%), 2 staphylococcus hominis (0.5%) and 1 staphylococcus simulans (0.2%). all three staphylococcus schleiferi strains were i ... | 1994 | 7889974 |
activation of the human immunodeficiency virus long terminal repeat in thp-1 cells by a staphylococcal extracellular product. | staphylococcal strains can release a factor that strongly activates the human immunodeficiency virus type 1 (hiv-1) long terminal repeat (ltr) in thp-1 cells transfected with the hiv-1 ltr-driven luciferase reporter gene (thp-1 ltrluc). the factor is present in the overnight culture fluid and is readily released from the organisms into aqueous medium by vigorous mixing. staphylococcal extracellular material is a complex mixture of polysaccharide and protein containing peptidoglycan and teichoic ... | 1994 | 7938001 |
isolation and identification of cheese-smear bacteria inhibitory to listeria spp. | a newly developed hydrophobic grid membrane method was used to rapidly screen 105 traditional french cheeses for surface smear microorganisms inhibitory to listeria monocytogenes strain v7. approximately 125,000 colonies comprising a wide variety of bacteria were examined of which less than 0.1% produced visible zones of inhibition. isolates possessing antilisterial activity consisted of various strains of enterococcus faecalis, staphylococcus xylosus, staphylococcus warneri and coryneform bacte ... | 1994 | 8024975 |
staphylococcus pasteuri sp. nov., isolated from human, animal, and food specimens. | a new novobiocin-susceptible species of the genus staphylococcus, staphylococcus pasteuri, is described on the basis of the results of a study of seven strains isolated from human, animal, and food specimens. dna relatedness experiments (s1 nuclease method) showed that these strains form a homogeneous genomic species related at dna homology levels of 2 to 13% to 27 type strains representing known staphylococcus species. the use of a method based on rrna gene restriction site polymorphism provide ... | 1993 | 8098615 |
use of liposome-immunopotentiated exopolysaccharide as a component of an ovine mastitis staphylococcal vaccine. | experiments on the development of a vaccine against staphylococcal mastitis were carried out in ewes. the vaccine (spanish patent no. 9200223) has the following components: (i) inactivated (formalinized) bacteria (staphylococcus aureus and a coagulase-negative staphylococcal species. staphylococcus simulans) and s. aureus toxoid in presence of an adjuvant (dextran sulfate, mw 500,000); and (ii) s. aureus exopolysaccharide included within liposomes. high serum antibody titres were obtained agains ... | 1994 | 8165857 |
studies on mutational cross-resistance between ciprofloxacin, novobiocin and coumermycin in escherichia coli and staphylococcus warneri. | nalidixic acid resistant mutants of escherichia coli kl16 were tested against ciprofloxacin, coumermycin and novobiocin. the mutants gyra, nalb and nal-24 were more resistant than kl16 to ciprofloxacin, whereas the nal-31 strain was hypersensitive. only the nalb mutant was more resistant to novobiocin than kl16, but gyra, nal-31 and nal-24 mutants were more sensitive to coumermycin than kl16. newly-isolated novobiocin-resistant mutants of kl16 were not cross-resistant to coumermycin or ciproflox ... | 1993 | 8246811 |
enterococci and staphylococci isolates from rumen of fallow deers and their antimicrobial activity. | this work presents enterococci and staphylococci isolated from the rumen content of fallow deers, their characteristics and antimicrobial activity. on the basis of biochemical tests, strains isolated were allotted to the species enterococcus solitarius, e. malodoratus, staphylococcus warneri, s. aureus, s. sciuri subsp. sciuri and s. xylosus. all strains produce lactic acid in the range from 0.303 to 0.500 mol.l-1. isolated strains were found to be resistant or sensitive to the antimicrobial add ... | 1993 | 8264425 |
study of the antiseptic efficacy of a quick drying rubbing type povidone-iodine alcoholic disinfectant solution by the glove juice method. | the antiseptic efficacy of had hand wash (83% ethanol containing 0.5% povidone-iodine) was investigated by the glove-juice method in 29 cases amongst staff members of our university. the number of viable bacteria on the hands and fingers was counted three times, and the mean of these counts was used as the baseline value for each subject. the number of viable bacteria on both hands was the same prior to disinfection. after the hands were disinfected by rubbing with 3 ml of had hand wash until it ... | 1993 | 8290451 |
[antagonistic activity of the rumen bacteria, enterococcus faecium and staphylococcus warneri]. | the bacterial strains e. faecium and s. warneri were tested for their antagonistic activities, and/or for the antimicrobial spectrum of bacteriocin-like substances produced by these bacteria. isolates of rumen and non-rumen origin were used as indicator strains (tab. i). all the isolates (except the strains sw48 and ef24c as indicators of rumen origin) produced bacteriocin-like substances inhibiting the growth of at least one of the series of the nine indicators used while clear inhibition zones ... | 1993 | 8346614 |
[properties of adherent staphylococci isolated from the rumen wall in lambs]. | six defined strains of adherent, coagulase-negative and catalase-positive staphylococci were isolated from the rumen wall of lambs. all the strains fermented maltose and had positive acetoin production. the strains were classified as follows on the basis of diagnostic tests, according to an identification key: three strains belonged to the species staphylococcus warneri (sw34, sw64, sw6), two to the species s. epidermidis (se30, se49) and one to the species s. cohnii subsp. urealyticum (scu32)-- ... | 1993 | 8488628 |
in vitro and in vivo antibacterial activities of s-1090, a new oral cephalosporin. | s-1090, a new oral cephalosporin, was active against selected gram-negative bacteria and methicillin-susceptible clinical isolates of staphylococcus aureus, staphylococcus epidermidis, and staphylococcus warneri, against which it had excellent activity. s-1090 was the most active compound against streptococcus pyogenes and streptococcus agalactiae among the agents compared. the in vivo efficacy of s-1090 against systemic and urinary and respiratory tract infections caused by gram-positive and -n ... | 1995 | 8585742 |
a simple and inexpensive method for the identification of staphylococcus epidermidis and staphylococcus hominis. | eight hundred and ninety-two strains of staphylococcus species were identified by means of desferrioxamine susceptibility and fermentation results of three carbohydrates, with the api staph system (biomérieux, france) as reference method. no identification could be obtained for 34 strains with api staph. of the remaining 858 strains, identical identification was obtained with 842 (98.1%). all 707 strains identified as staphylococcus epidermidis or staphylococcus hominis by the api staph system w ... | 1995 | 8681979 |
coagulase-negative staphylococci in danish blood cultures: species distribution and antibiotic susceptibility. | the distribution and antibiotic susceptibility of coagulase-negative staphylococci (cons) isolated from blood cultures was examined in samples from hospitals covering most of denmark. a total of 499 cons isolates were detected in 477 blood cultures from 340 patients and speciated as staphylococcus epidermidis, 285; staphylococcus hominis, 61; staphylococcus haemolyticus, 43; staphylococcus warneri, 12; staphylococcus cohnii, 7; staphylococcus saprophyticus, 4; staphylococcus capitis, 2 and staph ... | 1996 | 8690885 |
coagulase-negative staphylococci in air samples from operating theatres. | the aim of our study was to recognize coagulase-negative staphylococci (cns) in the air of operating theatres. out of the identification of 449 isolates, the most frequent species were staphylococcus epidermidis and staphylococcus haemolyticus. the strains were adherent to glass in 52.3%. most of the s. epidermidis showed adherent growth, while the majority of the s. haemolyticus failed to adhere. the disk-diffusion antibiotic sensitivity tests showed great differences in sensitivity to penicill ... | 1996 | 8806942 |
differentiation of bacterial 16s rrna genes and intergenic regions and mycobacterium tuberculosis katg genes by structure-specific endonuclease cleavage. | we describe here a new approach for analyzing nucleic acid sequences using a structure-specific endonuclease, cleavase i. we have applied this technique to the detection and localization of mutations associated with isoniazid resistance in mycobacterium tuberculosis and for differentiating bacterial genera, species and strains. the technique described here is based on the observation that single strands of dnas can assume defined conformations, which can be detected and cleaved by structure-spec ... | 1996 | 8940459 |
pediatric bacteremia due to staphylococcus warneri: microbiological, epidemiological, and clinical features. | between 1991 and 1995, an apparent high rate of staphylococcus warneri bacteremias at the royal children's hospital, melbourne, victoria, australia, raised the possibility of a virulent nosocomial strain. in a retrospective review of 30 s. warneri bacteremias in children, organisms were viable and verified in 22 episodes, 12 representing significant bacteremias. of these 12 episodes, 2 pairs shared chromosomal dna pulsed-field gel electrophoresis patterns in unconnected patients, dispelling conc ... | 1997 | 9230410 |
clinical and microbiological features of refractory periodontitis subjects. | the purpose of this investigation was to compare the clinical parameters and the site prevalence and levels of 40 subgingival species in successfully treated and refractory periodontitis subjects. 94 subjects received scaling and root planing and if needed, periodontal surgery and systemically administered tetracycline. 28 refractory subjects showed mean full mouth attachment loss and/or > 3 sites showing attachment loss > 2.5 mm within 1 year post-therapy. 66 successfully treated subjects showe ... | 1998 | 9495617 |
regulation of agr-dependent virulence genes in staphylococcus aureus by rnaiii from coagulase-negative staphylococci. | many of the genes coding for extracellular toxins, enzymes, and cell surface proteins in staphylococcus aureus are regulated by a 510-nucleotide (nt) rna molecule, rnaiii. transcription of genes encoding secreted toxins and enzymes, including hla (alpha-toxin), saeb (enterotoxin b), tst (toxic shock syndrome toxin 1), and ssp (serine protease), is stimulated, while transcription of genes encoding cell surface proteins, like spa (protein a) and fnb (fibronectin binding proteins), is repressed. be ... | 1998 | 9620969 |
an inexpensive and reliable method for routine identification of staphylococcal species. | the aim of this study was to develop a simple, reliable, and inexpensive in-house system for routine species identification of staphylococci in clinical practice. the system combines 15 key tests (including carbohydrate fermentation) performed in micro-well strips and antimicrobial disk diffusion susceptibility tests performed on standardised paper disk method antibiotic sensitivity medium agar. twenty-eight staphylococcal reference strains belonging to 18 different species were correctly identi ... | 1998 | 9721961 |
identification of staphylococcus carnosus and staphylococcus warneri isolated from meat by fluorescent in situ hybridization with 16s rrna-targeted oligonucleotide probes. | 16s rrna targeted oligonucleotide probes were designed by sequence analysis of an rrna database to discriminate s. carnosus, s. warneri, and s. saprophyticus species. after establishing hybridization conditions by rna dot blot hybridization with reference species, our probes were shown to be specific. by in situ hybridization only s-s-s.carno-0440-a-a-23 and s-s-s.war-0180-a-a-23 can specifically detect s. carnosus and s. warneri, respectively. the detection of old cells of s. carnosus 833 was m ... | 1999 | 10390873 |
bacterial degradation of hydrocarbons as evidenced by respirometric analysis. | the microbial biodegradability of mineral oil and other hydrocarbons, namely hexane, decane and tetradecane was determined using the warburg constant volume respirometer. results of oxygen uptake indicated that hexane and tetradecane were more degradable than mineral oil and decane. rhodococcus erythropolis and erwinia cancerogena showed the highest (0.866) and lowest (0.115) oxygen quotient (qo2) values, respectively, when exposed to mineral oil. staphylococcus warneri and enterobacter cloacae ... | 1999 | 10582377 |
rapid identification of staphylococcus epidermidis. | during the collection of airborne bacteria in a museum in england some bacterial strains were isolated which due to their fatty acid profiles were clearly identified as members of the genus staphylococcus. as fatty acid compositions of coagulase-negative staphylococci are very similar, differing only in quantities but not in qualities, further identification at the species level without a fatty acid database was not achieved. investigation of the isolates using the staph id 32 api system resulte ... | 2000 | 10843049 |
antimicrobial activity of bark extracts of syzygium jambos (l.) alston (myrtaceae). | syzygium jambos (l.) alston (myrtaceae) is a widespread medicinal plant traditionally used in sub-saharan africa to treat infectious diseases. acetone and aqueous extracts from the bark of s. jambos were tested for antimicrobial activity in vitro by the agar dilution method in petri dishes. both extracts showed some activity against the tested micro-organisms. they proved to be particularly effective on staphylococcus aureus, yersinia enterocolitica and coagulase negative staphylococci among whi ... | 2000 | 10904178 |
potential use of presumptive enterococci and staphylococci as indicators of sanitary condition in plants making hard italian-type cheese. | raw milk, pasteurized milk, unripened cheese (1 day old), and partially ripened cheese (3 months) from 42 milk lots at a plant making hard italian-type cheese were analyzed for presumptive enterococci using kanamycin esculin azide agar pour plates. fully ripened (> or =10 months) cheeses, derived from other milk lots, were also tested. numbers of presumptive staphylococci (baird-parker agar [b-p]) were determined in the partially and fully ripened cheeses. presumptive enterococci were ubiquitous ... | 2000 | 11131893 |
a novel lantibiotic, nukacin isk-1, of staphylococcus warneri isk-1: cloning of the structural gene and identification of the structure. | staphylococcus warneri isk-1, which we had previously reported as pediococcus sp. isk-1, produces a novel bacteriocin, nukacin isk-1. edman degradation of the chemically reduced nukacin isk-1 produced a sequence of 27 amino acids, 7 of which were unidentified. using single-specific-primer-pcr product as a probe, a 3.6-kb hindiii fragment containing the nukacin isk-1 structural gene (nuka) was cloned and sequenced. the deduced amino acid sequence of nukacin isk-1 had 57 amino acids, including a 3 ... | 2000 | 11193411 |
[bacteriologic evaluation of middle ear fluid during the course of secretory otitis media in children]. | fluid collected from tympanic cavity during operation from 43 children with o.m.s. was subjected to bacteriological examination. after the collection, each sample was immediately applied to bactec peds plus/f liquid medium from becton dickinson. the number of samples, where each genus and species of bacteria were found, was determined, as well as the number of samples where every two genus coexisted; also the ph genes, in which each genus was found were calculated. obtained results showed that 2 ... | 2000 | 11202340 |
cloning, purification and characterisation of staphylococcus warneri lipase 2. | a gene encoding an extracellular lipase was identified in staphylococcus warneri 863. the deduced lipase is organised as a prepro-protein and has significant similarity to other staphylococcal lipases. the mature part of the lipase was expressed with an n-terminal histidine tag in escherichia coli, purified and biochemically characterised. the results show that the purified lipase (named swl2) combines the properties of the staphylococcal lipases characterised so far. it has both a high preferen ... | 2001 | 11341932 |
bacterial flora of the conjunctiva and nasal cavity in normal and diseased captive bustards. | a survey was carried out to describe the normal aerobic bacterial flora of the conjunctiva and nasal cavity of captive houbara bustards (chlamydotis undulata), kori bustards (ardeotis kori), and white-bellied bustards (eupodotis senegalensis) maintained at the national avian research center, abu dhabi, united arab emirates. a total of 58 samples were examined from the nasal cavity and 55 samples from the conjunctiva of healthy bustards. there was no bacterial growth in 45% of conjunctival sample ... | 2001 | 11417826 |
early prosthetic valve endocarditis due to staphylococcus warneri with negative blood culture. | | 2001 | 11531329 |
the effect of osmotic stress on the production of nukacin isk-1 from staphylococcus warneri isk-1. | the effects of several additives on the production of a lantibiotic, nukacin isk-1, from staphylococcus warneri isk-1, in batch fermentation were studied. nacl, kcl and sorbitol stimulated nukacin isk-1 production. the addition of 1.4 m nacl increased nukacin isk-1 activity 1.5-fold over the control, while cell growth and glucose consumption were inhibited. nukacin isk-1 production increased with increasing osmolarity of the medium up to about 3 osmol/kg; however, further increases in osmolarity ... | 2001 | 11549026 |
comparison of the vitek gram-positive susceptibility 106 card, the mrsa-screen latex agglutination test, and meca analysis for detecting oxacillin resistance in a geographically diverse collection of clinical isolates of coagulase-negative staphylococci. | the vitek automated susceptibility testing system with a modified gram-positive susceptibility (gps) 106 card (biomerieux vitek, inc., hazelwood. mo.) and a rapid slide latex agglutination test (mrsa-screen test; denka seiken co., ltd., tokyo, japan) were evaluated for their abilities to detect oxacillin resistance in coagulase-negative staphylococci (cons). the reference broth microdilution method and the detection of the meca gene by pcr ("gold standard" reference result) were used to compare ... | 2001 | 11574584 |
genetic and biochemical characterization of glutamyl endopeptidase of staphylococcus warneri m. | a staphylococcus warneri strain m, newly isolated from processed seafood (smoked watasenia scintillans), produced an extracellular protease. the protease, designated to as m-prom (the mature form of prom), selectively cleaved the carbonyl side of glutamic acid residues in beta-casein. sequence of n-terminal 27 amino acids of m-prom, ranvilpnndrhqindttlghyapvtf, was found to be similar to those of other glutamyl endopeptidases, v8 protease (staphylococcus aureus strain v8) and spase (s. aureus at ... | 2001 | 11750133 |
staphylococcus warneri septicemia in preterm neonates - a reminder. | | 2002 | 11910146 |
concurrent serious bacterial infections in 2396 infants and children hospitalized with respiratory syncytial virus lower respiratory tract infections. | at driscoll children's hospital (corpus christi, tex), we observed that most infants and children hospitalized for treatment of respiratory syncytial virus (rsv) bronchiolitis and/or pneumonia received broad-spectrum intravenous antibiotics despite having typical rsv signs and symptoms and positive rsv-rapid-antigen tests on admission. physicians were concerned about the possibility of concurrent serious bacterial infections, especially in infants younger than 3 months and in those with infiltra ... | 2002 | 11929363 |
[diagnostic and therapeutic problem of infected arteriovenous fistula for hemodialysis-case report]. | on the case of 48 old patient with chronic renal failure, treated with repeated hemodialyses, we described diagnostic and therapeutic problems of infected arterio-venous fistula of severe clinical course and rare epidemiology (staphylococcus warneri). special attention was paid to the difficulties in the confirmation of bacterial etiology of this infection as well as practical usefulness of ultrasonography in the monitoring of clinical course and therapy of infected arteriovenous fistula. | 2001 | 12026519 |
[clinical and laboratory studies of bacterial adhesion to validate the choice of material for making provisional dentures for patients with periodontal diseases]. | adhesion of bacteria favoring the development of oral inflammations, including cariesogenic and periodontopathogenic (actinobacillus actinomycetemcommitans, streptococcus sanguis, fusobacterium nucleatum, staphylococcus warneri) and yeast fungi (candida albicans), to 13 materials used for making provisional dentures was studied. adhesion of all the studied bacteria and fungi to russian material esterfil foto was the minimum. clinical use of this material in patients with chronic generalized peri ... | 2002 | 12056141 |
[isolation of staphylococcus warneri from clinical samples and relationship to infection]. | | 2002 | 12325320 |
molecular analysis of the plasmid-borne aaca/aphd resistance gene region of coagulase-negative staphylococci from chickens. | the aim of this study was to analyse selected coagulase-negative staphylococci from chickens for the genetic basis of plasmid-borne resistance to gentamicin, tobramycin and kanamycin, and also for the presence of tn4001-like elements. | 2003 | 12716781 |
microbial quality and direct pcr identification of lactic acid bacteria and nonpathogenic staphylococci from artisanal low-acid sausages. | detection of six species of lactic acid bacteria and six species of gram-positive catalase-positive cocci from low-acid fermented sausages (fuets and chorizos) was assessed by species-specific pcr. without enrichment, lactobacillus sakei and lactobacillus curvatus were detected in 11.8% of the samples, and lactobacillus plantarum and staphylococcus xylosus were detected in 17.6%. enriched samples allowed the detection of l. sakei and s. xylosus in all of the samples (100%) and of enterococcus fa ... | 2003 | 12902246 |
production of a wide-spectrum antibacterial factor by staphylococcus warneri cells. | | 2003 | 12940166 |
staphylococcus warneri bw 94--a new source of lipase. | staphylococcus isolated from a common indian sweet viz. basundi was tested for its ability to produce lipase. the colorless zone of hydrolysis around the colony grown on baird parker agar containing egg yolk produced extracellular lipase. colony morphology, coagulase production, haemolysis, acid production in carbohydrate medium and enzyme activity studies showed that the organism was staphylococcus warneri. growth of s. warneri was obtained after 11 hr at 37 degrees c, ph 7.5, while the maximum ... | 2002 | 13677632 |
decreased vancomycin susceptibility of coagulase-negative staphylococci in a neonatal intensive care unit: evidence of spread of staphylococcus warneri. | coagulase-negative staphylococci (cons) are important pathogens in premature neonates; decreasing glycopeptide susceptibility has been observed among these isolates. the epidemiology of colonization with cons, the organisms' vancomycin susceptibilities, and genetic relatedness were studied over 6 months in a tertiary-care neonatal unit. a total of 321 isolates of cons were isolated. seventy-five percent of the infants were colonized at admission, and virtually all were colonized thereafter. comm ... | 2003 | 14532199 |
microbial community structure of membrane fouling film in an intermittently and continuously aerated submerged membrane bioreactor treating domestic wastewater. | there was an observable difference in microbial community structure between suspended microorganisms and membrane biofouling film in intermittently and continuously aerated smbrs. the dominant quinone type of membrane biofouling film in an intermittently aerated smbr was ubiquinone (uqs)-8, -10 followed by menaquinone (mks)-8(h4) and -8(h2). but that of the continuously aerated smbr was uqs-10, -8 followed by mks-6 and -8(h4). the experimental results also showed that the conditions of an interm ... | 2004 | 14982188 |
carcinogenic potential of tobacco tar-resistant staphylococcus aureus in buccal cavity. | the effects of cigarette smoking on the association between inflammation and cancer were studied, since some bacteria induce the production of tumor necrosis factor-alpha (tnf-alpha), a proinflammatory cytokine and endogenous tumor promoter, in cells. | 2004 | 15014984 |
determination of minimal regrowth concentration (mrc) in clinical isolates of various biofilm-forming bacteria. | based on the ability to attach to polymeric surfaces, the formation of biofilms was determined in 5 wild-type strains (pseudomonas aeruginosa, klebsiella pneumoniae, acinetobacter baumanii, escherichia coli, staphylococcus warneri). using modified christensen method, minimum regrowth concentration (mrc) of piperacillin, piperacillin-tazobactam, cefoperazon, ceftazidim, cefepim, meronem, ciprofloxacin, netilmicin and amikacin for gram-negative and of ampicillin-sulbactam, chloramphenicol, tetracy ... | 2004 | 15114870 |
multifocal discitis caused by staphylococcus warneri. | staphylococcus warneri is a coagulase-negative staphylococcus that is a normal inhabitant of the skin but occasionally causes septicemia and endocarditis. we report a case of multifocal discitis caused by s. warneri in an immunocompetent patient. only three cases of spinal s. warneri infections have been reported in the literature. they illustrate the atypical clinical presentation, with chronic pain of increasing severity in the thoracic or lumbar spine instead of the abrupt onset that characte ... | 2004 | 15182799 |
antimicrobial-resistance and enterotoxin-encoding genes among staphylococci isolated from expressed human breast milk. | resistance traits and the presence of enterotoxin-encoding genes were investigated in staphylococcus isolates obtained from expressed human breast milk. a total of 54 staphylococcal isolates identified as staphylococcus epidermidis (53.6 %), staphylococcus warneri (20.4 %), staphylococcus haemolyticus (13 %) and staphylococcus aureus (13 %) were investigated. by using a disc-diffusion method, higher rates of resistance, including intermediate resistance, were observed for penicillin (87 %) and e ... | 2004 | 15272063 |
characterization of a gene cluster of staphylococcus warneri isk-1 encoding the biosynthesis of and immunity to the lantibiotic, nukacin isk-1. | we characterized a gene cluster in a plasmid designated ppi-1 of staphylococcus warneri isk-1 encoding the biosynthesis of and immunity to the lacticin-481 type lantibiotic, nukacin isk-1. the dna sequence suggested that the nukacin isk-1 gene cluster consists of at least six genes, nuka (a structural gene), -m, -t, -f, -e, -g, and two open reading frames, orf1 and orf7. nukm and nukt were predicted to be involved in post-translational modification and secretion of nukacin isk-1 respectively. nu ... | 2004 | 15322349 |
icaa is not a useful diagnostic marker for prosthetic joint infection. | a collection of 99 staphylococcal isolates associated with prosthetic joint infection and 23 coagulase-negative staphylococci isolated from noninfected arthroplasty-associated specimens were screened in order to determine whether the presence of icaa could be used to distinguish between pathogens and nonpathogens. all staphylococcus aureus prosthetic joint infection isolates (n = 55) were icaa positive. a total of 46% (20 out of 44) of coagulase-negative staphylococcal prosthetic joint infection ... | 2004 | 15472359 |
evaluation of six commercial identification kits for the identification of staphylococcus aureus isolated from bovine mastitis. | comparison of six commercially available in human medicine well-established slide agglutination systems for the identification of staphylococcus aureus. | 2005 | 15659199 |
identification of coagulase-negative staphylococci other than staphylococcus epidermidis by automated ribotyping. | as routine identification of coagulase-negative staphylococci is problematic, the performance of automated ribotyping was evaluated for identification of coagulase-negative staphylococci other than staphylococcus epidermidis. in total, 177 isolates were tested, comprising 149 isolates from blood samples, 15 isolates that were not identified by internal transcribed spacer (its)-pcr in a previous study, and 13 reference strains. the identification results were compared with those obtained by the a ... | 2005 | 15715714 |
description of complete dna sequence of two plasmids from the nukacin isk-1 producer, staphylococcus warneri isk-1. | we report the whole dna sequence of two plasmids, ppi-1 (30.2 kb) and ppi-2 (2.8 kb). these plasmids are from staphylococcus warneri isk-1, which produces a lantibiotic, nukacin isk-1. curing of ppi-1 resulted in a loss of bactericidal activity in the culture supernatant and the host's immunity to nukacin isk-1, suggesting that the biosynthetic genes of the bacteriocin are encoded by ppi-1. based on the results of a homology search of each open reading flame, ppi-1 is comprised of the following ... | 2005 | 15737403 |
the two-component cell lysis genes holwmy and lyswmy of the staphylococcus warneri m phage varphiwmy: cloning, sequencing, expression, and mutational analysis in escherichia coli. | from the genome library of staphylococcus warneri m, the two successive cell-lysis genes (holwmy and lytwmy) were cloned and characterized. the lytwmy gene encoded a protein (lyswmy), whose calculated molecular mass and pi were 54 kda and 8.95, respectively. when overproduced in escherichia coli, lyswmy directed a protein of 45 kda (smaller than the predicted molecular mass), having n-terminal 13 residues identical with those predicted from dna. comparative analysis revealed that lyswmy signific ... | 2005 | 15848115 |
developing microbial spoilage population in vacuum-packaged charcoal-broiled european river lamprey (lampetra fluviatilis). | microbiological and sensory changes in vacuum-packaged charcoal-broiled river lampreys from three lamprey processing plants were monitored as a function of time at 8 degrees c. the lampreys were examined every 7 days up to 8 weeks for aerobic plate count (apc) and lactic acid bacteria (lab). the highest mean apc and lab were 6.01 log cfu/g and 4.86 log cfu/g, respectively. only 6 out of 15 lots reached an apc value of 7.0 log cfu/g during storage. the sensory scores remained at the baseline leve ... | 2004 | 15862876 |
staphylococcus warneri endocarditis after implantation of a lumbar disc prosthesis in an immunocompetent patient. | in a 48-year-old immunocompetent man with recurrent fever since 21 months, endocarditis of the native aortic valve due to staphylococcus warneri was diagnosed. twenty-six months before, a prosthetic lumbar disc had been implanted in l 4/5. the lack of typical other infectious sources favours the hypothesis of a disc-prosthesis-induced bacteraemia. | 2006 | 15904964 |
[the dependence of the antibacterial effect of the polycationic peptide warnerin on the energy state of target cells]. | the bactericidal effect of the polycationic peptide warnerin, produced by staphylococcus warneri iegm kl-1, was found to depend on the energy state of susceptible staphylococcus epidermidis cells. the pre-treatment of these cells with compounds that diminish the proton-motive force of plasma membranes enhanced cell tolerance to warnerin. the components deltapsi and deltaph of the membrane proton potential influenced the antibacterial activity of warnerin in different ways. in particular, the ant ... | 2005 | 15938391 |
effects of a topical essential oil-containing formulation on biofilm-forming coagulase-negative staphylococci. | to evaluate the antimicrobial effects of polytoxinol (pt), a topical essential oil-based formulation, against biofilm positive strains of coagulase-negative staphylococci. | 2005 | 15960752 |
purification and characterization of warnericin rb4, anti-alicyclobacillus bacteriocin, produced by staphylococcus warneri rb4. | in this study we characterized a bacteriocin, warnericin rb4, produced by staphylococcus warneri rb4. warnericin rb4 activity was completely inactivated by trypsin and actinase e. the activity was stable at 100 degrees c for 15 min, and had a ph range of 2 to 6. s. warneri rb4 showed antibacterial activity against only alicyclobacillus acidoterrestris, a. acidocaldarius, and micrococcus luteus, among 34 bacterial species tested. the amino acid sequence of the purified bacteriocin contained 27 am ... | 2005 | 15971094 |
characterization of functional domains of lantibiotic-binding immunity protein, nukh, from staphylococcus warneri isk-1. | the immunity to a lantibiotic, nukacin isk-1, is conferred by nukfeg (abc transporter) and nukh (lantibiotic-binding protein) cooperatively. the present study identifies the functional domains of nukh. the topological analysis indicated that nukh possesses two external loops and three transmembrane helices. deletion of n or c terminus of nukh did not affect the function. amino acids substitutions in the respective loops abolished the function. deletion of the third transmembrane helix resulted i ... | 2005 | 16009508 |
localization and interaction of the biosynthetic proteins for the lantibiotic, nukacin isk-1. | nukacin isk-1 is a type-a(ii) lantibiotic produced by staphylococcus warneri isk-1. in this study, we characterized nukm and nukt, which are predicted to be involved in modification of prepeptide (nuka) and cleavage of leader peptide and subsequent secretion respectively. localization analysis of nukm and nukt in the wild-type strain indicated that both proteins were located at the cytoplasm membrane. interestingly, nukm expressed heterologously in st. carnosus tm300 was also located at the cyto ... | 2005 | 16041140 |
a novel type of immunity protein, nukh, for the lantibiotic nukacin isk-1 produced by staphylococcus warneri isk-1. | staphylococcus warneri isk-1 produces a lantibiotic, nukacin isk-1. the nukacin isk-1 gene cluster consists of at least six genes, nuka, -m, -t, -f, -e, and -g, and two open reading frames, orf1 and orf7 (designated nukh). sequence comparisons suggested that nukf, -e, -g, and -h contribute to immunity to nukacin isk-1. we investigated the immunity levels of recombinant lactococcus lactis expressing nukfeg and nukh against nukacin isk-1. the co-expression of nukfeg and nukh resulted in a high deg ... | 2005 | 16041148 |
staphylococcus capitis endocarditis due to a transvenous endocardial pacemaker infection: case report and review of staphylococcus capitis endocarditis. | newer microbiologic methods to determine the species of coagulase-negative staphylococci (cons) have evolved which have shown that most endocarditis due to cons is caused by staphylococcus epidermidis, and far fewer by staphylococcus warneri and staphylococcus lugdunensis. | 2005 | 16085441 |
widespread distribution of disinfectant resistance genes among staphylococci of bovine and caprine origin in norway. | we demonstrate here a widespread distribution of genes mediating efflux-based resistance to quaternary ammonium compounds (qacs) in staphylococci from unpasteurized milk from 127 dairy cattle herds and 70 dairy goat herds. qac resistance genes were identified in 21% of the cattle herds (qaca/b, smr, qacg, and qacj) and in 10% of the goat herds (qaca/b and smr). further examination of 42 qac-resistant bovine and caprine isolates revealed the following genes: qaca/b (12 isolates) was present in fo ... | 2005 | 16145078 |
isolation and characterization of a staphylococcus warneri strain producing an anti-legionella peptide. | legionella pneumophila is a pathogenic bacterium found in freshwater environments that is responsible for pneumonia. people become infected through inhalation of contaminated droplets from water devices, such as cooling towers and showers. it is important to find new treatments that decrease the development of legionella. we found a staphylococcus warneri strain that inhibits legionella growth. this activity is due to a molecule secreted by s. warneri. this molecule displayed a high heat-stabili ... | 2005 | 16227110 |
heterologous expression and functional analysis of the gene cluster for the biosynthesis of and immunity to the lantibiotic, nukacin isk-1. | nukacin isk-1 is a lantibiotic produced by staphylococcus warneri isk-1. the gene cluster of nukacin isk-1 consists of at least nukamtfeg, orf1 and orf7. in this study, we demonstrated the heterologous production of nukacin isk-1 in lactococcus lactis by the artificial polycistronic expression of nukamtfeg-orf7 under the control of the nisin-controlled expression (nice) system. consequently, the recombinant l. lactis showed antimicrobial activity. mass analysis clarified the presence of nukacin ... | 2004 | 16233732 |
deletion of pt181-like sequence in an smr-encoding mosaic plasmid harboured by a persistent bovine staphylococcus warneri strain. | the aim was to study the persistence and characteristics of staphylococcus warneri strains resistant to quaternary ammonium compounds (qacs), including sequencing and analysis of two plasmids proved to carry the smr gene. | 2006 | 16286359 |