| binding and cross-linking of tmrna to ribosomal protein s1, on and off the escherichia coli ribosome. | uv irradiation of an in vitro translation mixture induced cross-linking of 4-thiou-substituted tmrna to escherichia coli ribosomes by forming covalent complexes with ribosomal protein s1 and 16s rrna. in the absence of s1, tmrna was unable to bind and label ribosomal components. mobility assays on native gels demonstrated that protein s1 bound to tmrna with an apparent binding constant of 1 x 10(8) m(-1). a mutant tmrna, lacking the tag coding region and pseudoknots pk2, pk3 and pk4, did not com ... | 2000 | 11101533 |
| glyceraldehyde-3-phosphate dehydrogenase-encoding gene as a useful taxonomic tool for staphylococcus spp. | the gap gene of staphylococcus aureus, encoding glyceraldehyde-3-phosphate dehydrogenase, was used as a target to amplify a 933-bp dna fragment by pcr with a pair of primers 26 and 25 nucleotides in length. pcr products, detected by agarose gel electrophoresis, were also amplified from 12 staphylococcus spp. analyzed previously. hybridization with an internal 279-bp dna fragment probe was positive in all pcr-positive samples. no pcr products were amplified when other gram-positive and gram-negat ... | 2000 | 11101563 |
| capillary gas chromatography with chemical ionization negative ion mass spectrometry in the identification of odorous steroids formed in metabolic studies of the sulphates of androsterone, dha and 5alpha-androst-16-en-3beta-ol with human axillary bacterial isolates. | the products of metabolism of the sulphates (0.5 micromol/l) of androsterone, dehydroepiandrosterone (dha) and 5alpha-androst-16-en-3beta-ol have been investigated after incubation with 72 h cultures of human axillary bacterial isolates for 3 days at 37 degrees c. the medium used, tryptone soya broth (tsb), contained yeast extract and tween 80. the isolates used were coryneform f1 (known previously to metabolize testosterone and to be involved in under-arm odour (uao) production, i.e. uao +ve), ... | 1997 | 9449209 |
| identification of endogenous ssra-tagged proteins reveals tagging at positions corresponding to stop codons. | the ssra.smpb quality control system adds a c-terminal degradation peptide (aandenyalaa) to nascent chains on stalled ribosomes, thereby freeing the ribosome and ensuring proteolysis of the tagged protein. an ssra mutant with the tag sequence aandehhhhhh was used to slow degradation and facilitate ni2+-nitrilotriacetic acid affinity purification. display of affinity-purified escherichia coli proteins on two-dimensional gels revealed small quantities of a diverse set of ssra-h6-tagged proteins, a ... | 2001 | 11373298 |
| multiple staphylococcal cassette chromosomes and allelic variants of cassette chromosome recombinases in staphylococcus aureus and coagulase-negative staphylococci from norway. | we investigated the nature of the staphylococcal cassette chromosome mec (sccmec) elements and cognate insertion sites in a collection of 42 clinical staphylococcal isolates of various species from norway. the ccr and mec genes and the attachment sites (attl/attr) were identified by pcr, southern blot hybridization, and dna sequencing. we found 10 possibly new sccmec types and one previously unreported variant of sccmec type iii (mec complex a, ccrab3, and ccrc7) in staphylococcus epidermidis, s ... | 2007 | 17307983 |
| bacteremia in cirrhotic patients submitted to endoscopic band ligation of esophageal varices. | endoscopic procedures can develop bacteremia. patients with chronic liver disease are more predisposed to undergo bacteremia and infections because they are immunocompromised. | 2004 | 15029392 |
| [pyomyositis, sacroiliitis and spondylodiscitis caused by staphylococcus hominis in a immunocompetent woman]. | in absence of risk factors, osteoarticular infections by coagulase-negative staphylococci are very infrequent. we described the case of a immunocompetent 73-year-old-woman that suffered pyomyositis, left sacroiliitis and spondylodiscitis involving the first and second thoracic vertebrae by staphylococcus hominis. this multifocal infection occurred five-weeks after intramuscular administration of nsai for treatment of low back pain associated with a herniated disc l4-l5. this is the first know ca ... | 2006 | 17371146 |
| a new approach towards ikimine a analogues. | an analogon of the alkylpyridine alkaloid ikimine a (1) was prepared in six steps starting from undec-10-ynoic acid. a key step in this synthesis was a sonogashira coupling of the alkyne and 3-iodopyridine, followed by hydrogenation of the alkyne, reduction of the ester to the primary alcohol and oxidation to the corresponding aldehyde. this aldehyde was converted to the ikimine a analogon with o-methyl hydroxylamine hydrochloride. this product and the intermediate alkylpyridines were tested in ... | 2004 | 15248605 |
| a case of delayed brain abscess due to a retained intracranial wooden foreign body: a case report and review of the last 20 years. | a 13-year-old female is presented. when she was six years old, she had fallen, holding wooden chopsticks and got stuck with a chopstick in the right upper eyelid. she was brought to a physician immediately, but a residual foreign body was missed and no particular symptom had developed during 7 years. she visited our department with fever and headache, and a brain abscess and an intracranial foreign body were found on computed tomography (ct) and magnetic resonance image (mri) 7 years after the p ... | 2004 | 15254807 |
| [microbiology of acute exacerbation chronic sinusitis in adults]. | the bacterial flora taken by sinus puncture from 115 patients with uni and bilateral acute exacerbation of chronic maxillary sinusitis was analyzed. germ-free culture was obtained in 49 cases (40%) and in 69 cases (60%) bacteria were isolated from all sinus aspirates. microbiological evaluation shows, that in majority of cases (90%) of acute exacerbation of chronic sinusitis there were pathogenic bacteria namely aerobic and anaerobic. the most frequent pathogens isolated from the aspirates were; ... | 2004 | 15307480 |
| the etiologic diagnosis of infectious discitis is improved by amplification-based dna analysis. | blood cultures and cultures of disc material are required to identify and treat bacterial agents responsible for septic spondylodiscitis, but these methods have limited sensitivities. we undertook this study to compare nonculture amplification-based dna analysis with conventional culture of disc aspirate. | 2004 | 15457468 |
| icaa is not a useful diagnostic marker for prosthetic joint infection. | a collection of 99 staphylococcal isolates associated with prosthetic joint infection and 23 coagulase-negative staphylococci isolated from noninfected arthroplasty-associated specimens were screened in order to determine whether the presence of icaa could be used to distinguish between pathogens and nonpathogens. all staphylococcus aureus prosthetic joint infection isolates (n = 55) were icaa positive. a total of 46% (20 out of 44) of coagulase-negative staphylococcal prosthetic joint infection ... | 2004 | 15472359 |
| evaluation of phenotypic methods for methicillin resistance characterization in coagulase-negative staphylococci (cns). | coagulase-negative staphylococci (cns) are the major cause of nosocomial infections. methicillin-resistant strains are particularly important because they narrow therapeutic options. detecting methicillin resistance among cns has been a challenge for years. the objective of this study was to determine the accuracy of an agar screening test (0.6 and 4 microg oxacillin ml(-1)), disc diffusion and the automated microscan system to characterize methicillin resistance among cns. one hundred and seven ... | 2004 | 15585497 |
| identification of coagulase-negative staphylococci other than staphylococcus epidermidis by automated ribotyping. | as routine identification of coagulase-negative staphylococci is problematic, the performance of automated ribotyping was evaluated for identification of coagulase-negative staphylococci other than staphylococcus epidermidis. in total, 177 isolates were tested, comprising 149 isolates from blood samples, 15 isolates that were not identified by internal transcribed spacer (its)-pcr in a previous study, and 13 reference strains. the identification results were compared with those obtained by the a ... | 2005 | 15715714 |
| successful liver and kidney transplantation from cadaveric donors with left-sided bacterial endocarditis. | bacterial infections are frequent in cadaveric organ donors and can be transmitted to the transplantation recipient, which could have devastating consequences for the recipients if adequate preventive measures are not adopted. from the 355 consecutive brain dead cadaveric organ donors procured at our center in the last four years, 2000-2003, four of them (1.1%) had bacterial endocarditis as cause of death. the bacteria responsible for the endocarditis were staphylococcus epidermidis, coagulase-n ... | 2005 | 15760402 |
| inhibitory effect of gram-negative and gram-positive microorganisms against helicobacter pylori clinical isolates. | to determine the in vitro inhibitory effect of several gram-negative and gram-positive microorganisms against helicobacter pylori clinical isolates. | 2008 | 17965421 |
| bacterial contamination of random-donor platelets in a university hospital in the midwestern region of brazil. | bacterial blood contamination was probably the first recognized transfusion-transmitted disease. although the transfusion-associated bacterial sepsis has been known for a long time, it remains an important health problem. at present it is the most frequently reported cause of infectious transfusion-related fatalities. the aims of the study were to determine the prevalence of microbiologic contamination in random-donor platelets (rdps) and to identify the isolated microorganism obtained from a br ... | 2008 | 18005324 |
| a comparison of adherence by four strains of staphylococcus intermedius and staphylococcus hominis to canine corneocytes collected from normal dogs and dogs suffering from atopic dermatitis. | the aim of this study was to compare the adherence of four strains of staphylococcus intermedius and a single strain of staphylococcus hominis to corneocytes from both normal dogs and dogs suffering from atopic dermatitis. cells from the skin surface, corneocytes, were collected from 10 normal dogs and 10 dogs suffering from atopic dermatitis. four strains of s. intermedius, three isolated from canine pyoderma skin lesions (strains a, b and c), and one isolated form from canine synovial membrane ... | 2005 | 15766937 |
| staphylococcus hominis endophthalmitis associated with a capsular hypopyon. | to report a case of staphylococcus hominis endophthalmitis associated with a capsular hypopyon. | 2005 | 15860311 |
| antimicrobial activity and tightness of a dcpd-cao-based hydraulic calcium phosphate cement for root canal filling. | calcium hydroxide is currently used in dentistry for endodontic treatments where its main advantage is its antibacterial and anti-inflammatory activity. however, it also has some drawbacks such as pulp necrosis, slight solubility, slow and insufficient hardening, and retraction on drying. in consequence, it is used only as temporary material for root canal disinfection. by mixing calcium hydrogen phosphate dihydrate (cahpo4 . 2h2o, also called dicalcium phosphate dihydrate, dcpd) and calcium oxi ... | 2005 | 15981171 |
| comparative in vitro activity of l-ofloxacin and fk037 to other agents against 10,040 fresh clinical isolates. | fresh clinical isolates collected from november 1, 1992 through november 1, 1993, were tested by agar dilution against 26 different antimicrobial agents including fk037 and l-ofloxacin. among the 10 040 organisms tested were staphylococcus aureus (n = 1222), methicillin-resistant staphylococcus aureus (mrsa, n = 455), staphylococcus epidermidis (n = 533), staphylococcus hominis (n = 90), staphylococcus hemolyticus (n = 89), streptococcus pneumoniae (n = 144), escherichia coli (n = 2326), klebsie ... | 1996 | 18611710 |
| widespread distribution of disinfectant resistance genes among staphylococci of bovine and caprine origin in norway. | we demonstrate here a widespread distribution of genes mediating efflux-based resistance to quaternary ammonium compounds (qacs) in staphylococci from unpasteurized milk from 127 dairy cattle herds and 70 dairy goat herds. qac resistance genes were identified in 21% of the cattle herds (qaca/b, smr, qacg, and qacj) and in 10% of the goat herds (qaca/b and smr). further examination of 42 qac-resistant bovine and caprine isolates revealed the following genes: qaca/b (12 isolates) was present in fo ... | 2005 | 16145078 |
| nosocomial spread of a staphylococcus hominis subsp. novobiosepticus strain causing sepsis in a neonatal intensive care unit. | from 2002 to 2003, 32 isolates of staphylococcus hominis subsp. novobiosepticus (shn) were recovered from 21 patients, 18 of whom were neonates, with 13 considered to have late-onset shn sepsis. all isolates from neonates had an indistinguishable pulsed-field gel electrophoresis pattern. our data support shn as an important nosocomial pathogen in neonates. | 2005 | 16145165 |
| pacemaker lead endocarditis caused by staphylococcus hominis. | infective endocarditis related to pacemaker is a rare but serious condition in permanent venous tracing. a 65-year-old man was admitted to the hospital with high fever and chills. a ddd pacemaker had been implanted via the right subclavian vein because of sick sinus syndrome 6 years earlier. transesophageal echocardiogram identified an oscillating round hyperechoic mass with a stalk near the tricuspid valve. blood cultures grew staphylococcus hominis. the patient was treated with antibiotics and ... | 2006 | 16689853 |
| diversity of staphylococcal cassette chromosome mec structures in methicillin-resistant staphylococcus epidermidis and staphylococcus haemolyticus strains among outpatients from four countries. | in staphylococci, methicillin (meticillin) resistance (mr) is mediated by the acquisition of the meca gene, which is carried on the size and composition variable staphylococcal cassette chromosome mec (sccmec). mr has been extensively studied in staphylococcus aureus, but little is known about mr coagulase-negative staphylococci (mr-cons). here, we describe the diversity of sccmec structures in mr-cons from outpatients living in countries with contrasting environments: algeria, mali, moldova, an ... | 2009 | 19001111 |
| comparison of three phenotypic techniques for detection of methicillin resistance in staphylococcus spp. reveals a species-dependent performance. | to evaluate the usefulness of the cefoxitin screen in vitek 2 gram-positive panels for recognizing methicillin-resistant strains of staphylococci. | 2009 | 19136679 |
| comparison of two commercial methods with pcr restriction fragment length polymorphism of the tuf gene in the identification of coagulase-negative staphylococci. | two commercial methods for the identification of coagulase-negative staphylococci (cns) were compared with the restriction fragment length polymorphism (rflp) of the amplified tuf gene, which served as the reference method. | 2006 | 16965378 |
| staphylococcal and micrococcal adherence to canine and feline corneocytes: quantification using a simple adhesion assay. | in this paper a simple adhesion assay suitable for the assessment of bacterial adhesion to both canine and feline corneocytes is described. using this assay staphylococcus intermedius, staphylococcus aureus and staphylococcus chromogenes were shown to adhere well to both canine and feline corneocytes. the numbers of adherent bacteria were, however, generally lower for feline corneocytes. both staphylococcus hominis and a micrococcus species adhered poorly to canine and feline corneocytes. this i ... | 2007 | 17222237 |
| evaluation of mucosal and seborrheic sites for staphylococci in two populations of captive psittacines. | to survey 2 populations of psittacines to characterize staphylococcus spp isolated from commensal cutaneous microflora. | 2009 | 19335240 |
| alcohol increases hemolysis by staphylococci. | it was recently found that alcohols can confer hemolytic properties on certain species of yeast. here, it is reported that alcohol can promote hemolysis by various species of staphylococci, including strains of staphylococcus aureus, staphylococcus epidermidis and staphylococcus hominis. in order to study this novel phenomenon in s. aureus and s. epidermidis, strains that exhibit this phenomenon (e.g. s. aureus 8325-4, col, sh1000, s. epidermidis), as compared with strains that exhibited little ... | 2007 | 17227454 |
| volatile constituents of bracts and leaves of wild and cultivated origanum dictamnus. | the chemical composition of the volatile constituents from bracts and leaves of wild and hydroponically cultivated origanum dictomnus were analysed by gc and gc-ms. three different levels of nitrogen (100,150, 200 mg/l), were used in the nutrient solution for the cultivation, using the nutrient film technique (n.f.t). carvacrol was the predominant compound in all cases. the essential oils were investigated for their antimicrobial activity against staphylococcus aureus, staphylococcus epidermidis ... | 1999 | 17260258 |
| [comparative activity of daptomycin against clinical isolates of methicillin-resistant staphylococcus aureus and coagulase-negative staphylococci]. | the objective of this study was to determine the in vitro activity of daptomycin and other agents against methicillin-resistant staphylococcus aureus (mrsa) isolates from several sources and coagulase-negative staphylococci (cons) from clinically significant blood cultures. | 2010 | 19406529 |
| identification of staphylococcus species with the vitek 2 system: the case of staphylococcus hominis. | | 2007 | 17277175 |
| interventions for control of salmonella: clearance of microbial growth from rubber picker fingers. | a study was conducted to determine if a surface material with antimicrobial properties combined with an effective disinfectant could achieve total clearance of bacterial contamination. before beginning the project, new rubber picker fingers collected from 3 processing facilities were tested for endogenous microflora. five species of bacteria common to soil and human handling were present: bacillus amyloliquefaciens, bacillus cereus/thuringiensis, staphylococcus epidermidis, staphylococcus homini ... | 2009 | 19439642 |
| bacteriology of infected extracted pacemaker and icd leads. | pacemaker and implantable cardioverter defibrillator infections, when not treated, lead to serious consequences. the aim is to identify the prevalent strains of the responsible bacteria to guide an effective therapy. | 2009 | 19512941 |
| renal tolerability of teicoplanin in a case of neonatal overdose. | the literature does not contain reports regarding teicoplanin overdose in newborns. in a neonate with a history of recent postasphyctic acute renal failure which recovered within 7 days of life, antibiotic therapy with teicoplanin was started for sepsis due to staphylococcus hominis. however, for 5 days the dosage was excessive (20 mg/kg twice daily instead of an initial dose of 16 mg/kg and then doses of 8 mg/kg once daily). once this error had been noted, therapy was immediately suspended. cli ... | 1998 | 9822356 |
| molecular techniques open up new vistas for typing of coagulase-negative staphylococci. | several methods were used to type 64 clinical isolates of coagulase-negative staphylococci (cns) derived from hospitals in morocco. the clinical isolates originated principally from blood cultures and wound sources. these isolates provided the opportunity to substantially compare the proficiency of developing molecular techniques with conventional phenotypic tests for use in the identification of clinical staphylococci. the following molecular methods were examined: utility ribotyping analysis ( ... | 2001 | 11367554 |
| coagulase-negative staphylococci isolated from various skin lesions. | we isolated 162 coagulase-negative staphylococci (48: from infection, 114: from colonization) from various skin diseases between january, 1995, and january, 1998. from eighteen infected cysts, 10 staphylococcus epidermidis strains, 3 s. capitis strains, 2 s. hominis strains, 2 s. auricularis strains, and one s. saprophyticus strain were individually detected. similarly, from ten folliculitis lesions, 6 s. epidermidis strains, 2 s. capitis strains, and 2 s. hominis strains, and from five furuncle ... | 1998 | 9798341 |
| probiotic potential of staphylococcus hominis mbbl 2-9 as anti-staphylococcus aureus agent isolated from the vaginal microbiota of a healthy woman. | to isolate and characterize an antagonist for use as probiotic agent in the biocontrol of staphylococcus aureus. | 2010 | 19709336 |
| detection of new mutations conferring resistance to linezolid in glycopeptide-intermediate susceptibility staphylococcus hominis subspecies hominis circulating in an intensive care unit. | glycopeptides and linezolid are the most widely used antibiotics to treat infections by methicillin-resistant staphylococcus spp. we report the presence of various isolates of methicillin-resistant s. hominis subsp. hominis with resistance to linezolid and reduced susceptibility to glycopeptides. we studied ten blood culture isolates of s. hominis subsp. hominis from nine patients admitted to our hospital. etest was used to study susceptibility to antibiotics commonly prescribed against staphylo ... | 2010 | 19876662 |
| detection and characterization of erythromycin-resistant methylase genes in gram-positive bacteria isolated from poultry litter. | the epidemiology of four erythromycin-resistant methylase ( erm) genes, erma, ermb, ermc and msra, was determined in erythromycin-resistant staphylococci, enterococci and streptococci isolated from poultry litter. all isolates were resistant to multiple antibiotics. southern hybridization indicated that 4 of the 20 staphylococci contained the ermc gene on plasmids: on a 2.2 kb plasmid in staphylococcus hominis and s. sciuri, on a 6.0 kb plasmid in s. xylosus, and on a 7.0 kb plasmid in s. lentus ... | 2002 | 12111173 |
| genotypic and phenotypic properties of coagulase-negative staphylococci causing dialysis catheter-related sepsis. | sixty coagulase-negative staphylococcus (cns) isolates were recovered from the blood cultures or peritoneal dialysate effluent of 43 patients on renal dialysis. the patients had either renal dialysis catheter-related sepsis (crs) or continuous ambulatory peritoneal dialysis (capd)-associated peritonitis. isolates were characterized by biotyping, and genotyped by pulsed-field gel electrophoresis (pfge). phenotypic properties of the strains were also investigated. several genotypes were identified ... | 2003 | 12919757 |
| use of the vitek 2 system for rapid identification of clinical isolates of staphylococci from bloodstream infections. | staphylococci are an increasing cause of bloodstream infections. rapid reliable identification of these organisms is essential for accurate diagnosis and prompt effective treatment. we evaluated the ability of the vitek 2 system (biomérieux, inc, hazelwood, mo.) to identify these organisms rapidly and accurately. a total of 405 clinically relevant nonduplicate staphylococcal isolates (staphylococcus aureus, n = 130; coagulase-negative staphylococci, n = 275) collected from blood cultures were te ... | 2003 | 12958254 |
| application of molecular typing methods to characterize nosocomial coagulase-negative staphylococci collected in a greek hospital during a three-year period (1998-2000). | a total of 143 methicillin-resistant coagulase-negative staphylococci (mr-cns) collected between 1998 and 2000 at the university hospital of patras, greece, were characterized by antibiogram and genomic typing to define the clonal types endemic in this hospital and their evolution during the 3-year period. these isolates corresponded to 93 methicillin-resistant staphylococcus epidermidis (mrse) and 50 other mr-cns, which were isolated from patients in different wards, exclusively from blood and ... | 2003 | 12959406 |
| staphplex system for rapid and simultaneous identification of antibiotic resistance determinants and panton-valentine leukocidin detection of staphylococci from positive blood cultures. | phenotypic methods take several days for identification and antimicrobial susceptibility testing of staphylococcal isolates after gram-positive cocci in clusters (gpcc) are observed in positive blood cultures. we developed and validated a staphplex system that amplifies and detects 18 gene targets simultaneously in 1 reaction for species-level identification of staphylococci, detection of genes encoding panton-valentine leukocidin (pvl), and antimicrobial resistance determinants of staphylococci ... | 2007 | 17446323 |
| antibiotic resistance patterns of coagulase-negative staphylococcus strains isolated from blood cultures of septicemic patients in turkey. | the aim of this study is to determine antibiotic resistance patterns and slime production characteristics of coagulase-negative staphylococci (cons) caused nosocomial bacteremia. a total of 200 cons strains were isolated from blood samples of patients with true bacteremia who were hospitalized in intensive care units and in other departments of istanbul university cerrahpasa medical hospital between 1999 and 2006. among 200 cons isolates, staphylococcus epidermidis was the most prevalent species ... | 2009 | 17475456 |
| distribution and expression of macrolide resistance genes in coagulase-negative staphylococci. | in total, 494 isolates of coagulase-negative staphylococci (cons) were identified to the species level by biochemical tests and soda sequencing. erythromycin resistance phenotypes were determined and specific resistance genes were identified by pcr. the prevalence of erythromycin resistance varied widely among staphylococcal species, from 0% in staphylococcus lugdunensis to almost 90% in staphylococcus haemolyticus. most (63%) erythromycin-resistant isolates carried constitutively expressed erm( ... | 2007 | 17501977 |
| changes in histamine and microbiological analyses in fresh and frozen tuna muscle during temperature abuse. | temperature abuse of tuna (thunnus alalunga) was carried out in order to assess the histamine buildup in fish-processing facilities where fish can be exposed to high temperatures for short periods of time. histamine production was studied in tuna loins under different storage and abuse conditions. tuna was stored at 0-2 degrees c, 3-4 degrees c, and 6-7 degrees c, and abused for 2 h daily at 20 degrees c and 30 degrees c for 7-12 days. loins abused at 30 degrees c for 2 h daily contained potenti ... | 2007 | 17613069 |
| dna methylase modifications and other linezolid resistance mutations in coagulase-negative staphylococci in italy. | despite 10 years of clinical use, linezolid resistance in staphylococcus aureus and coagulase-negative staphylococci (cons) is still a rare phenomenon. this study reports the mechanisms of resistance and strain types seen in clusters of linezolid-resistant cons from two different hospitals in italy during the period 2008-09. | 2010 | 20852270 |
| ovarian hydrobursitis in female camels (camelus dromedaries): biochemical, bacterial and protozoal evaluation. | the aim of this study was to evaluate female camels affected with ovarian hydrobursitis (n = 31) for hematological and biochemical findings and for bacterial and protozoal infections. blood samples were obtained and surgical ablation of the affected bursa was performed. bursal fluid, follicular fluid, and serum were subjected to hormonal and biochemical analyses. bursal fluids were cultured and colonies were identified using biomérieux vitek two compact system. passive haemagglutination test was ... | 2010 | 21144565 |
| differentiation of staphylococcus spp. by high-resolution melting analysis. | high-resolution melting analysis (hrma) is a fast (post-pcr) high-throughput method to scan for sequence variations in a target gene. the aim of this study was to test the potential of hrma to distinguish particular bacterial species of the staphylococcus genus even when using a broad-range pcr within the 16s rrna gene where sequence differences are minimal. genomic dna samples isolated from 12 reference staphylococcal strains (staphylococcus aureus, staphylococcus capitis, staphylococcus caprae ... | 2010 | 21164574 |
| bacterial contamination of water in dental unit reservoirs. | the aim of this study was bacteriological assessment of water in dental unit reservoirs--concentration and composition of the aerobe and facultative anaerobe bacterial microflora. reservoir water samples were taken from 25 units. bacterial flora were determined with the plate culture method. bacteria were identified with biochemical microtests: api 20e, api 20ne (biomérieux, france) and gp2 microplatetm (biolog, usa). the concentration of total bacteria isolated from one site was 201,039 cfu/ml, ... | 2007 | 17655191 |
| staphylococcus hominis native mitral valve bacterial endocarditis (sbe) in a patient with hypertrophic obstructive cardiomyopathy. | there are several species of coagulase-negative staphylococci (cons) that are part of the normal skin flora and are relatively noninvasive/low virulence organisms. cons are important pathogens in patients with prosthetic devices and are the most common pathogen associated with prosthetic valve endocarditis. cons native valve infective endocarditis (ie) is rare. patients with hypertrophic obstructive cardiomyopathy and an outflow pressure gradient greater than 30 mm hg are predisposed to ie. ther ... | 2007 | 17845885 |
| [nosocomial spread of linezolid-resistant staphylococcus hominis in two hospitals in majorca]. | since march 2008, several linezolid and teicoplanin-resistant staphylococcus hominis (s. hominis) isolates have been recovered from patients admitted to the two major hospitals on the island of majorca, spain. for this reason, a study was conducted to determine the molecular epidemiology of these isolates and the mechanism of linezolid resistance. | 2011 | 21435748 |
| evolution of slime production by coagulase-negative staphylococci and enterotoxigenic characteristics of staphylococcus aureus strains isolated from various human clinical specimens. | the present study was designed to determine the slime production of coagulase-negative staphylococci (cons) and the enterotoxigenic properties of staphylococcus aureus strains, and to evaluate the clinical importance of slime-producing cons and enterotoxigenic s. aureus strains isolated from various human clinical specimens. for this purpose, a total of 120 staphylococcus strains were isolated and identified, and further characterized for their slime production and enterotoxigenicity. of the cli ... | 2007 | 17893164 |
| distribution of staphylococcal cassette chromosome mec (sccmec) types i, ii, iii and iv in coagulase-negative staphylococci from patients attending a tertiary hospital in southern brazil. | coagulase-negative staphylococci (cons) are now recognized as the aetiological agents of an important range of infections in humans. most developed countries have reported an increase in cons infections in hospitalized patients that are resistant to meticillin and other antibiotics. staphylococcal cassette chromosome mec (sccmec) typing is essential for understanding the molecular epidemiology of meticillin-resistant staphylococcus strains. sccmec elements are currently classified into types i t ... | 2007 | 17893169 |
| occurrence of staphylococci in the oral cavities of healthy adults and nasal oral trafficking of the bacteria. | to investigate a possible peroral route of infective endocarditis (ie), the occurrence of staphylococci in the oral cavity was examined using saliva and supragingival plaque specimens from 56 systemically and periodontally healthy adults aged 22-43 years old (27.1+/-5.3). nine staphylococcus species and 334 isolates were identified. in saliva, the total occurrence rate was 83.9 % and the total number of bacteria was 10(2)-10(4) c.f.u. ml(-1). staphylococcus aureus was the most frequent species ( ... | 2008 | 18065673 |
| outbreak of staphylococcus hominis subsp. novobiosepticus bloodstream infections in são paulo city, brazil. | | 2008 | 18201999 |
| clonal relatedness of methicillin-resistant coagulase-negative staphylococci in the haemodialysis unit of a single university centre in greece. | coagulase-negative staphylococci (cons) are frequently encountered pathogens in hospital environment. dialysis patients, often carrying central venous catheters, are prone to cons infections. methicillin-resistant (mr) staphylococci in hospitals are resistant to multiple antibiotics and may cause an overall increase in the incidence of staphylococcal infections rather than simply replacing the more susceptible strains. the aim of this study was to evaluate the antimicrobial resistance and the cl ... | 2008 | 18305314 |
| identification of the nukacin kqu-131, a new type-a(ii) lantibiotic produced by staphylococcus hominis kqu-131 isolated from thai fermented fish product (pla-ra). | staphylococcus hominis kqu-131, isolated from thai fermented marine fish, produces a heat stable bacteriocin. structural and genetic analysis indicated that the bacteriocin is a variant of nukacin isk-1, a type-a(ii) lantibiotic, and we termed the bacteriocin nukacin kqu-131. there were three different amino acid residues between nukacin isk-1 and nukacin kqu-131, one residue in the leader peptide and the other two in the mature peptide. | 2008 | 18685189 |
| [severe congenital neutropenia]. | severe congenital neutropenia is a primary immunodeficiency; the lack of maturation of neutrophil precursor in bone marrow and severe neutropenia are the subjacent characteristics which explain a marked susceptibility to severe and recurrent infections; bacteria and fungi are the most common etiologic agents. we report the case of an infant with severe congenital neutropenia that began at 4 days from birth with perianal abscess infections, sepsis, liver abscess, gingivitis and oral ulcers; she r ... | 2010 | 21854729 |
| staphylococcus hominis subsp. novobiosepticus strains causing nosocomial bloodstream infection in brazil. | to report the isolation of six staphylococcus hominis subsp. novobiosepticus (shn) strains from hospitalized patients with bloodstream infections in two brazilian hospitals and to characterize their susceptibility profile to several antimicrobials. | 2008 | 18775890 |
| prevalence and persistence of coagulase-negative staphylococcus species in three dairy research herds. | coagulase-negative staphylococcus species (cns) were isolated from 11.3% (1407 of 12,412) of mammary quarter milk samples obtained from cows in three dairy research herds in 2005. approximately 27% (383/1407) of cns was identified to the species level. the species distribution among those cns identified from all herds was staphylococcus chromogenes (48%), staphylococcus hyicus (26%), staphylococcus epidermidis (10%), staphylococcus simulans (7%), staphylococcus warneri (2%), staphylococcus homin ... | 2009 | 18950962 |
| transient bacteremia after removal of a bonded maxillary expansion appliance. | the aim of this study was to investigate the presence of transient bacteremia after the removal of a modified bonded rapid maxillary expansion appliance. | 2009 | 19201325 |
| exopolysaccharide production by lactose-hydrolyzing bacteria isolated from traditionally fermented milk. | with increasing consumer demands for safer, healthier and more natural products, bacterially produced exopolysaccharides (epss) are becoming a viable option as an additive in designer-type foods. fresh milk samples from cattle and sheep were collected from informal settlements in south africa. after a three day incubation period at 25 degrees c, 550 bacterial strains were isolated and evaluated for eps production from lactose as sole carbon source. strains producing eps on lactose were identifie ... | 2009 | 19282044 |
| reliable identification of clinically prevalent species and subspecies of staphylococci by sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis. | we identified by sodium dodecyl sulfate polyacrylamide gel electrophoresis (sds-page) 10 species and 5 subspecies of staphylococcus among 139 clinical isolates and compared it with conventional tests. all isolates showed species-specific whole-cell protein profiles, even atypical strains, with up to 60% and at least 73.5% of interspecies and intraspecies similarity, respectively. except for 5 isolates that presented biochemical profiles of staphylococcus hominis subsp. hominis, but were identifi ... | 2009 | 19362254 |
| infectious aortitis and spondylodiscitis in patients with endovascular stents. | the infection of endovascular stents remains one of the most problematic complications of aortic surgery. this article describes the case of a 61-year-old male with ascendant and descendent aorta endovascular stents, hospitalized for pyrexia, weight loss and back pain. blood culture was positive for staphylococcus hominis resistant to oxacillin and ciprofloxacin. spiral computed tomography, magnetic resonance imaging and leukocyte-labelled scintigraphy showed that the patient developed a perigra ... | 2009 | 19390503 |
| prevalence, intensity and identity of bacteraemia following conservative dental procedures in children. | the aim of this study was to investigate the prevalence, intensity and microbial identity of bacteraemia following conservative dental procedures. the procedures were placement of rubber dam, use of the fast drill, use of the slow drill and placement of a matrix band and wedge. | 2009 | 19416445 |
| diversity of staphylococcal cassette chromosome mec structures in coagulase-negative staphylococci and relationship to drug resistance. | the objective of this study was to determine the distribution of staphylococcal cassette chromosome mec (sccmec) elements in meticillin-resistant coagulase-negative staphylococci (mr-cons) isolated from a tertiary-care hospital in mexico and to examine the relationship to drug resistance. fifty selected mr-cons isolates collected from catheters (n=15), blood (n=15), bone (n=9), bronchial lavage (n=2) and urine (n=2) and one isolate each from an abscess, cerebrospinal fluid, eye, pleural effusion ... | 2010 | 20007762 |
| alternative therapies in antibiotic-resistant infection. | case report: a 24-year-old woman suffering from post-influenza otitis media infection was initially treated with several series of a steroid (elocon) and a combination of steroids and antibiotics (atecortin, dicortineff) without significant medical benefit. the isolated bacterial strains were identified as staphylococcus homis and staphylococcus epidermidis. specific phage therapy applied sequentially over a period of three weeks resulted only in a partial reduction in inflammation and limited i ... | 2006 | 17357317 |
| moluccensins h-j, 30-ketophragmalin limonoids from xylocarpus moluccensis. | three new phragmalin limonoids, moluccensins h-j (1-3), were isolated from seed kernels of the cedar mangrove, xylocarpus moluccensis. their structures were established by extensive spectroscopic analysis. compound 2 displayed weak antibacterial activity against staphylococcus hominis and enterococcus faecalis. | 2010 | 20112995 |
| bacterial bloodstream infections and antimicrobial susceptibility pattern in pediatric hematology/oncology patients after anticancer chemotherapy. | bloodstream infections in pediatric hematology and oncology represent a major problem worldwide, but this has not been studied in qatar. in this study, we investigated the burden of infection and the resistance pattern in the bacterial etiology, in the only tertiary pediatric hematology and oncology center in qatar. | 2014 | 25395866 |
| prevalence of resistant gram-negative bacilli in bloodstream infection in febrile neutropenia patients undergoing hematopoietic stem cell transplantation: a single center retrospective cohort study. | bloodstream infection (bsi) is an important cause of morbidity and mortality in patients undergoing hematopoietic stem cell transplantation (hsct). to evaluate the causative bacteria and identify risk factors for bsi associated mortality in febrile neutropenia patients undergoing hsct, we collected the clinical and microbiological data from patients underwent hsct between 2008 and 2014 and performed a retrospective analysis. throughout the study period, among 348 episodes of neutropenic fever in ... | 2015 | 26559260 |
| evaluation of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry in comparison to rpob gene sequencing for species identification of bloodstream infection staphylococcal isolates. | as a result of variable expression of biochemical characters, misidentification by conventional phenotypic means often occurs with clinical isolates belonging to staphylococcus species. therefore, we evaluated the use of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (maldi-tof ms) for the identification of 450 blood isolates of the most relevant staphylococcal species, using sequence analysis of the rpob gene as the reference method. a correct species identificatio ... | 2011 | 20132252 |
| maldi-tof mass spectrometry speciation of staphylococci and their discrimination from micrococci isolated from indoor air of schoolrooms. | the focus of our work is the identification of medically relevant staphylococci from the environment; these organisms are among the major opportunistic pathogens associated with human disease. andersen sampling was performed in schoolrooms during the school year. eleven of thirty six isolates (all gram-positive tetrads) were identified as staphylococci and 23 were characterized as micrococci. maldi-tof ms profiling was used as the first stage in the classification followed by standard biochemica ... | 2010 | 20383373 |
| biodegradability of commercial and weathered diesel oils. | this work aimed to evaluate the capability of different microorganisms to degrade commercial diesel oil in comparison to a weathered diesel oil collected from the groundwater at a petrol station. two microbiological methods were used for the biodegradability assessment: the technique based on the redox indicator 2,6 -dichlorophenol indophenol (dcpip) and soil respirometric experiments using biometer flasks. in the former we tested the bacterial cultures staphylococcus hominis, kocuria palustris, ... | 2008 | 24031193 |
| prevalence and antimicrobial susceptibility patterns of bacteria from milkmen and cows with clinical mastitis in and around kampala, uganda. | identification of pathogens associated with bovine mastitis is helpful in treatment and management decisions. however, such data from sub-saharan africa is scarce. here we describe the distribution and antimicrobial susceptibility patterns of bacteria from cows with clinical mastitis in kampala, uganda. due to high concern of zoonotic infections, isolates from milkmen are also described. | 2013 | 23667611 |
| activity of telavancin and comparator antimicrobial agents tested against staphylococcus spp. isolated from hospitalised patients in europe (2007-2008). | the activity of telavancin was evaluated against staphylococcus spp. collected from european hospitals as part of an international surveillance study (2007-2008). a total of 7534 staphylococcal clinical isolates [5726 staphylococcus aureus and 1808 coagulase-negative staphylococci (cons)] were included. isolates were tested for susceptibility according to reference methods and minimum inhibitory concentration (mic) values were interpreted based on clinical and laboratory standards institute (cls ... | 2010 | 20598860 |
| characterization and structure identification of an antimicrobial peptide, hominicin, produced by staphylococcus hominis mbbl 2-9. | hominicin, antimicrobial peptide displaying potent activity against staphylococcus aureus atcc 25923, methicillin-resistant s. aureus (mrsa) atcc 11435 and vancomycin-intermediate s. aureus (visa) ccarm 3501, was purified by chloroform extraction, ion-exchange column chromatography and reverse-phase hplc from culture supernatant of staphylococcushominis mbbl 2-9. hominicin exhibited heat stability up to 121 degrees c for 15min and activity under both acidic and basic conditions (from ph 2.0 to 1 ... | 2010 | 20654578 |
| new insight into microbial diversity and functions in traditional vietnamese alcoholic fermentation. | the roles of microorganisms in traditional alcoholic fermentation are often assumed based on abundance in the starter and activity in pure culture. there is a serious lack of hard evidence on the behavior and activity of individual microbial species during the actual fermentation process. in this study, microbial succession and metabolite changes during 7days of traditional vietnamese alcoholic fermentation were monitored. special attention was devoted to starch degradation. in total, 22 microbi ... | 2016 | 27236224 |
| actinomyces gerencseriae hip prosthesis infection: a case report. | actinomyces bacteria are part of the human oropharyngeal microbiota. they have been associated with abdominal, cervicofacial and thoracic infections and a few cases of joint infections have also been described. in particular, actinomyces gerencseriae, formerly described as actinomyces israelii serovar ii, has rarely been associated with human infections, mostly involving cervicofacial lesions and periodontal diseases. here, we report one case of hip prosthesis infection due to a. gerencseriae. | 2015 | 26415492 |
| rapid differentiation of staphylococcus aureus, staphylococcus epidermidis and other coagulase-negative staphylococci and meticillin susceptibility testing directly from growth-positive blood cultures by multiplex real-time pcr. | this study evaluated a multiplex real-time pcr method specific for the meca, fema-sa and fema-se genes for rapid identification of staphylococcus aureus, staphylococcus epidermidis and non-s. epidermidis coagulase-negative staphylococci (cons), and meticillin susceptibility testing directly in positive blood cultures that grew gram-positive cocci in clusters. a total of 100 positive blood cultures produced: 39 s. aureus [12 meticillin-resistant s. aureus (mrsa), 31% of all the s. aureus]; 30 s. ... | 2010 | 20813851 |
| susceptibility patterns of coagulase-negative staphylococci to several newer antimicrobial agents in comparison with vancomycin and oxacillin. | coagulase-negative staphylococci (cons) have emerged as important nosocomial pathogens. cons resistance to meticillin and other semisynthetic penicillins is now common. elevated vancomycin minimal inhibitory concentrations (mics) have been reported and are associated with worse treatment outcomes. several newer antibiotics have recently become available for the treatment of gram-positive infections. the purpose of this study was to assess the in vitro activity of telavancin, daptomycin, linezoli ... | 2011 | 21295951 |
| antibiotic resistance and molecular characterization of clinical isolates of methicillin-resistant coagulase-negative staphylococci isolated from bacteremic patients in oncohematology. | polymerase chain reaction (pcr) amplification of antibiotic resistance genes as well as staphylococcal cassette chromosome mec (sccmec) typing and pulsed-field gel electrophoresis (pfge) of smai macrorestriction fragments of genomic dna were used to characterize 45 methicillin-resistant coagulase-negative staphylococci (mrcons) isolates responsible of bacteremia recovered in patients at the bone marrow transplant centre of tunisia in 1998-2007. among the 45 mrcons isolates, staphylococcus epider ... | 2011 | 21431912 |
| procalcitonin levels in bloodstream infections caused by different sources and species of bacteria. | the aim of this study was to evaluate procalcitonin (pct) diagnostic accuracy in discriminating gram-negative (gn) from gram-positive (gp) bloodstream infections and determining the relationship between pct levels, infection sites, and pathogen types. | 2017 | 27979420 |
| conjunctival and intraocular swabs for the microbiological assessment of donor corneas. | in this study, we investigated the associations between conjunctival (co) and intraocular (io) swabs and their implications for the contamination rates of organ-cultured corneas. | 2016 | 26146765 |
| resistance of bacterial biofilms formed on stainless steel surface to disinfecting agent. | the natural ability of microorganisms for adhesion and biofilm formation on various surfaces is one of the factors causing the inefficiency of a disinfection agent, despite its proven activity in vitro. the aim of the study was to determine the effectiveness of disinfecting substances on bacterial biofilms formed on stainless steel surface. a universally applied disinfecting agent was used in the tests. bacterial strains: listeria innocua, pseudomonas putida, micrococcus luteus, staphylococcus h ... | 2010 | 21466046 |
| calcific myonecrosis of the leg: report of three patients presenting with infection. | abstract background: calcific myonecrosis is a rare late complication of trauma, affecting almost exclusively the lower limb. its radiologic appearance is characteristic. superimposed infection usually is a sequela of biopsy. case report: we present three patients, one with bilateral involvement, who presented with calcific myonecrosis and spontaneous infection. three infections were attributable to a single microorganism: enterobacter cloacae, staphylococcus hominis, and s. haemolyticus were re ... | 2011 | 21510773 |
| [investigation of reduced vancomycin susceptibility in methicillin-resistant staphylococci.] | the first staphylococcus aureus strain with reduced susceptibility to vancomycin was reported from japan in 1996, and since then an increasing numbers of cases had been reported from various countries. along with the unfeasibility in the identification of these strains with routine laboratory methods, the use of glycopeptid antibiotics in infections due to these strains may result in therapeutic failure. the aim of this study was to investigate the prevalence of vancomycin intermediate staphyloc ... | 2011 | 21644067 |
| activity of vancomycin, ciprofloxacin, daptomycin, and linezolid against coagulase-negative staphylococci bacteremia. | multiresistant coagulase-negative staphylococci (cns) infections are mainly increased in hospitalized patients. we have studied the activity of vancomycin, ciprofloxacin, daptomycin and linezolid in methicillin-resistant cns strains, isolated from true blood cultures. | 2011 | 21666998 |
| molecular detection of enterotoxins e, g, h and i in staphylococcus aureus and coagulase-negative staphylococci isolated from clinical samples of newborns in brazil. | aims:ôçé the objective of this study was to investigate the detection of see, seg, seh and sei in strains of staphylococcus aureus and coagulase-negative staphylococci (cns) using rt-pcr. methods and results:ôçé in this study, 90 staph.┬áaureus strains and 90 cns strains were analysed by pcr for the detection of genes encoding staphylococcal enterotoxins (se) e, g, h and i. one or more genes were detected in 54 (60%) staph.┬áaureus isolates and in 29 (32┬à2%) cns isolates. staphylococcus epiderm ... | 2011 | 21672099 |
| molecular epidemiology of methicillin-resistant staphylococcus hominis (mrsho): low clonality and reservoirs of sccmec structural elements. | methicillin resistant staphylococcus hominis (mrsho) are important human pathogens in immunocompromised patients. however, little is known regarding its population structure and staphylococcal chromosomal cassette mec (sccmec) content. | 2011 | 21760926 |
| zaaps program results for 2010: an activity and spectrum analysis of linezolid using clinical isolates from 75 medical centres in 24 countries. | the zyvox® annual appraisal of potency and spectrum (zaaps) program monitors the in vitro activities of linezolid and comparator agents for gram-positive organisms in latin america, europe, canada, and the asia-pacific. for the 2010 program a total of 6305 gram-positive strains were collected from 75 medical centres on five continents (24 countries). reference broth microdilution susceptibility tests were performed on organisms from the following groups: staphylococcus aureus (2875), coagulase n ... | 2012 | 23174097 |
| Antibiotic sensitivity of bacterial isolates from cases of canine dermatitis. | Among 97 bacterial isolates, 74 strains of Staphylococcus spp developed from 95 swabs taken from skin lesions in dogs. Twenty-eight staphylococcal strains resistant to methicillin and/or oxacillin were identified and mecA expression was confirmed for 14 of these strains. S. aureus and S. intermedius group (SIG) strains were particularly relevant in our cases due to their antibiotic resistance leading to an increased veterinary and public health risk. We suggest a diagnostic protocol based on cyt ... | 2011 | 22143814 |
| transcriptional profiling analysis of the global regulator norg, a gntr-like protein of staphylococcus aureus. | the gntr-like protein norg has been shown to affect staphylococcus aureus genes involved in resistance to quinolones and β-lactams, such as those encoding the norb and abca transporters. to identify the target genes regulated by norg, we carried out transcriptional-profiling assays using s. aureus rn6390 and its isogenic norg::cat mutant. our data showed that norg positively affected the transcription of global regulators mgra, arls, and sarz. the three putative drug efflux pump genes most posit ... | 2011 | 21908673 |
| microbiological and molecular characterization of human clinical isolates of staphylococcus cohnii, staphylococcus hominis, and staphylococcus sciuri. | the incidence of coagulase-negative staphylococci reported as causative agents of nosocomial infections has risen in the last decade. the aim of this study was to characterize biofilm formation, antibiotic resistance, sccmec type, and genetic relatedness in clinical isolates of staphylococcus cohnii, staphylococcus hominis, and staphylococcus sciuri recovered from humans. | 2011 | 21851333 |
| Eustachian valve endocarditis: Rare case reports and review of literature. | Eustachian valve endocarditis (EVE) is a distinctly rare and underdiagnosed entity. We report 2 new cases caused by vancomycin resistant Staphylococcus aureus and Staphylococcus hominis diagnosed on transesophageal echocardiography (TEE). Although, 63% of Eustachian valve endocarditis is caused by Staphylococcus aureus, we report the first case of vancomycin resistant Staphylococcus aureus and first case related to implantable venous access systems. EVE is now seen more commonly in elderly popul ... | 2011 | 22022147 |
| Species-specific sensitivity of coagulase-negative Staphylococci to single antibiotics and their combinations. | The activity of beta-lactam antibiotics (oxacillin, cloxacillin, cephalotin), vancomycin, gentamicin and rifampicin applied in vitro individually and in combination against 37 nosocomial methicillin-resistant strains of coagulase-negative staphylococci (CNS) was assessed to demonstrate the heterogeneity of this group of bacteria and estimate the chance of the efficacy of such therapy. The strains belonged to four species: Staphylococcus epidermidis, Staphylococcus haemolyticus, Staphylococcus co ... | 2011 | 21905634 |
| double triplex real-time pcr assay for simultaneous detection of staphylococcus aureus, staphylococcus epidermidis, staphylococcus hominis, and staphylococcus haemolyticus and determination of their methicillin resistance directly from positive blood culture bottles. | we developed and validated here a double triplex real-time pcr assay to simultaneously detect and identify staphylococcus aureus, staphylococcus epidermidis, staphylococcus hominis, staphylococcus haemolyticus and their methicillin resistance in a single reaction directly from gram-positive cocci-in-clusters (gpcs)-positive blood culture bottles. from august 15, 2009 through february 15, 2010, 238 gpc-positive samples were collected and identified by conventional methods as 11 methicillin-resist ... | 2011 | 21925597 |
| silver/chitosan/cellulose fibers foam composites: from synthesis to antibacterial properties. | chitosan, associated with cellulose fibers, can be used for elaborating sponge-like structures (membranes, foams) for the binding of silver ions. the composite material has very promising antibacterial properties versus pseudomonas aeruginosa (gram(-))≫escherichia coli (gram(-))>staphylococcus hominis (gram(+))≫staphylococcus aureus (gram(+)). the amount of silver required for bactericidal effect is quite low (below 0.1 mg per disk, this means less than 6 mg ag g(-1)) in antibiogram-type test bu ... | 2013 | 23245882 |