PMID(sorted descending)
recovering traditional raw-milk tetilla cheese flavour and sensory attributes by using kocuria varians and yarrowia lipolytica adjunct cultures.the rationale of the present study was to evaluate the potential of microbial adjunct cultures including kocuria varians and/or yarrowia lipolytica strains in the recovery of the typical sensory profile of traditional (raw-milk) tetilla cheese. four batches of tetilla cheese, a short ripened cows' milk cheese produced in galicia (nw spain), were made in duplicate from pasteurized milk inoculated with different microbial cultures. a control batch was manufactured by adding a mesophilic commercial ...201728384620
peripherally inserted central catheter-related bloodstream infection caused by kocuria marina in an elderly man.kocuria species are gram-positive, aerobic cocci, and members of the micrcoccaceae family that are known to be opportunistic pathogens. although there have been sporadic reports of infections caused by kocuria species, little is known regarding their human pathogenicity and clinical characteristics.201728102479
purification and partial identification of bacteriocin isk-1, a new lantibiotic produced by pediococcus sp. isk-1.bacteriocin isk-1 is a proteinaceous inhibitory substance produced by pediococcus sp. isk-1 isolated from well-aged nukadoko. bacteriocin isk-1 was purified by acid treatment, ammonium sulfate precipitation, cation-exchange chromatography, and reversed-phase hplc from the culture supernatant of pediococcus sp. isk-1. purification of bacteriocin isk-1 resulted in a 30-fold increase in the specific activity and the recovery was 17%. molecular mass of bacteriocin isk-1 measured by fast atom bombard ...199827392396
genome sequence of kocuria varians g6 isolated from a slaughterhouse in denmark.we report here the first draft genome sequence ofkocuria variansg6, which was isolated from a meat chopper at a small slaughterhouse in denmark. the 2.90-mb genome sequence consists of 95 contigs and contains 2,518 predicted protein-coding genes.201627034480
semen collection methods affect the bacterial composition of post-thawed semen of silver barb (barbodes gonionotus).biosafety issue associated with the risk of pathogenic contamination of cryopreserved semen is a common concern because of associated declines in sperm quality, storage period and disease transmission. this study was conducted to evaluate the effects of methods of semen collection on sperm quality and bacterial composition of post-thawed semen of silver barb (barbodes gonionotus). semen collection methods consisted of four treatments: (1) hand-stripping of abdomen without rinsing of urogenital a ...201626778122
effect of seed pelleting with biocontrol agents on growth and colonisation of roots of mungbean by root-infecting fungi.mungbean (vigna radiata (l.) wilczek) is a leguminous pulse crop that is a major source of proteins, vitamins and minerals. root-infecting fungi produce severe plant diseases like root rot, charcoal rot, damping-off and stem rot. the soil-borne pathogens can be controlled by chemicals, but these chemicals have several negative effects. use of microbial antagonist such as fungi and bacteria is a safe, effective and eco-friendly method for the control of many soil-borne pathogens. biological contr ...201626619828
effect of starter cultures and packaging methods on amino acid profile and eating quality characteristics of pork ham.wet cured pork hams were inoculated with a mixed starter cultures comprising of lactobacillus acidophilus and micrococcus varians m483 at the dose level of 106 cfu/g and the un inoculated hams served as controls. the amino acid profile of hams of the treated and the control groups stored at 4oc under map and vp and evaluated on 60th day of storage revealed that treated hams liberated higher concentration of free amino acids except for proline and methionine which were found in higher concentrati ...201526243961
conjunctival flora of clinically normal and diseased turtles and captive breed turtles and tortoises conjunctival disease is common. our aim was to investigate the bacterial and fungal flora present in the eyes of healthy and pathological chelonians and to compare findings in turtles with those in tortoises.201525889261
ability of kocuria varians lth 1540 to degrade putrescine: identification and characterization of a novel amine oxidase.this work describes the identification and characterization of an amine oxidase from kocuria varians lth 1540 (syn. micrococcus varians) primarily acting on putrescine. data from maldi-tof ms/ms and the identification of δ(1)-pyrroline as degradation product from putrescine indicate that the enzyme is a flavin-dependent putrescine oxidase (puo). properties of partially purified enzyme have been determined. the enzyme oxidizes diamines, putrescine and cadaverine, and, to a lesser extent, polyamin ...201525817823
catheter-related bacteremia caused by kocuria salsicia: the first case.we report the first case of catheter-related bacteremia caused by kocuria salsicia in a patient with short bowel syndrome. the pathogen was initially identified as kocuria varians by a vitek 2-based assessment, but its 16s rrna gene sequence showed 100% similarity to k. salsicia. the patient was successfully treated with vancomycin and removal of the catheter.201525497673
degradation of delor 103, a technical mixture of polychlorinated biphenyls, by selected bacteria.ability to utilize a technical mixture of polychlorinated biphenyls (pcb), delor 103, as the sole carbon source, has been tested in 14 bacterial strains. for the five best growing strains (alcaligenes latus, alcalgenes eutrophus, comamonas testosteroni, micrococcus varians and pseudomonas putida), the dependence of the degradation of individual pcb congeners on the number of chlorine substituents is discussed.199324420291
biodegradation of naphthalene and anthracene by chemo-tactically active rhizobacteria of populus deltoides.several naphthalene and anthracene degrading bacteria were isolated from rhizosphere of populus deltoides, which were growing in non-contaminated soil. among these, four isolates, i.e. kurthia sp., micrococcus varians, deinococcus radiodurans and bacillus circulans utilized chrysene, benzene, toluene and xylene, in addition to anthracene and naphthalene. kurthia sp and b. circulans showed positive chemotactic response for naphthalene and anthracene. the mean growth rate constant (k) of isolates ...201024031572
effect of starter culture and turmeric on physico-chemical quality of carabeef pastirma.carabeef samples were sliced, pressed, cured and divided into 6 groups. starter cultures (micrococcus varians m483 (mv), staphylococcus carnosus (sc), lactobacillus sakei (ls), m. varians m483+ lb. sakei and staph. carnosus + lb. sakei) were inoculated at the dose of 10(6)-0(7)cfu/g and stored at 10 ± 1°c for 7 days. uninoculated samples were maintained as control. samples were then divided into 2 treatment groups. samples of treatment 1 (t1) were smeared with a paste of turmeric followed by app ...201023572607
structure of starch binding domains of halophilic alpha-amylase at low ph.the solubility and structural properties of halophilic proteins are ascribed to their abundant acidic residues, resulting in large net negative charges at neutral ph. this study examined the effects of low ph, i.e., reduction of net negative charges on the structural properties of starch binding domain (sbd) of halophilic kocuria varians α-amylase. titration to ph 2.1 caused loss of 233 nm peak characteristic of aromatic interactions present in the native sbd at neutral ph and resulted in the sp ...201323033857
catheter-related relapsing peritonitis due to kocuria varians in a patient undergoing continuous ambulatory peritoneal dialysis. 201222806295
assessment of microbial diversity in biofilms recovered from endotracheal tubes using culture dependent and independent approaches.ventilator-associated pneumonia (vap) is a common nosocomial infection in mechanically ventilated patients. biofilm formation is one of the mechanisms through which the endotracheal tube (et) facilitates bacterial contamination of the lower airways. in the present study, we analyzed the composition of the et biofilm flora by means of culture dependent and culture independent (16 s rrna gene clone libraries and pyrosequencing) approaches. overall, the microbial diversity was high and members of d ...201222693635
comparison of aerosol and bioaerosol collection on air filters.air filters efficiency is usually determined by non-biological test aerosols, such as potassium chloride particles, arizona dust or di-ethyl-hexyl-sebacate (dehs) oily liquid. this research was undertaken to asses, if application of non-biological aerosols reflects air filters capacity to collect particles of biological origin. the collection efficiency for non-biological aerosol was tested with the palas set and iso fine test dust. flow rate during the filtration process was 720 l/h, and partic ...201122523449
polycyclic aromatic hydrocarbon-degrading bacteria from aviation fuel spill site at ibeno, nigeria.polycyclic aromatic hydrocarbon (pah)-degrading bacteria were isolated from aviation fuel contaminated soil at inua eyet ikot in ibeno, nigeria. pah-degrading bacteria in the contaminated soil were isolated by enrichment culture technique. isolates with high pah degrading potential characterized by their extensive growth on pah-supplemented minimal salt medium were screened for their naphthalene, phenanthrene and chrysene degradability. the screening medium which contained selected pahs as the s ...201222456728
effects of salt and ligand concentrations on the thermal unfolding and refolding of halophilic starch-binding domain from kocuria varians α-amylase.the starch binding domain of α-amlylase from moderate halophile was expressed in e. coli with his tag (his- sbd12) and characterized for its halophilic properties. his-sbd12 was stable up to 35°c and showed binding activity, although at reduced level, to amylose even in the absence of nacl. both nacl and specific ligands exhibited insignificant influence on the secondary structure of his-sbd12, but showed significant stabilization effects against thermal unfolding concentration-dependently, show ...201222409499
distinct characteristics of single starch-binding domain sbd1 derived from tandem domains sbd1-sbd2 of halophilic kocuria varians alpha-amylase.kocuria varians alpha-amylase contains tandem starch-binding domains sbd1-sbd2 (sbd12) that possess typical halophilic characteristics. recombinant tandem domains sbd12 and single domain sbd1, both with amino-terminal hexa-his tag, were expressed in and purified to homogeneity from escherichia coli. the circular dichroism (cd) spectrum of his-sbd12 was characterized by a positive peak at 233 nm ascribed to the aromatic stacking. although the signal occurred in the far uv region, it is an indicat ...201222388479
metabolism of nitrate in fermented meats: the characteristic feature of a specific group of fermented foods.within the universe of food fermentation processes the multi-purpose use of nitrate and/or nitrite is a unique characteristic of meat fermentations. these curing agents play a decisive role in obtaining the specific sensory properties, stability and hygienic safety of products such as fermented sausages, ham and, more recently, emulsion type of sausages. the use of nitrate is the traditional method in curing processes and requires its reduction to reactive nitrite. thus, nitrate reduction is the ...201222202868
analysis of biogenic amines in northern and southern european sausages and role of flora in amine production.the biogenic amine contents, microbial counts and flora producing amines were investigated in four types of fermented sausages. southern type european sausages (italian and belgian) showed higher tyramine and phenylethylamine values than northern type ones (norwegian and belgian). the spontaneous non-starter lactic acid bacteria could be responsible for the production of these amines in the italian products, and the cocci gram positive in the belgian south ones. the norwegian sausages showed the ...200222064002
behaviour of the constitutive biota of two types of spanish dry-sausages ripened in a pilot-scale chamber.the behaviour of the constitutive biota in eighty four samples belonging to two different types of spanish dry-cured sausages during the ripening process in a pilot-scale chamber was investigated. samples were analyzed in three stages during production: fresh product, first drying stage and finished product. lactic acid bacteria (lab) and coagulase-negative cocci (cnc) were identified by the api system. in general, evolution of lab and cnc during the ripening process of spanish dry-cured sausage ...200622062067
staphylococcal growth and enterotoxin production in the presence of meat cultures (non lab).the effect of meat cultures (non lactic acid bacteria) on the growth and production of enterotoxins and thermonuclease by staphylococcus aureus was studied. micrococcus varians did not affect growth nor the synthesis of metabolites. levels of enterotoxins a, b and d produced by the respective s. aureus strains were reduced by s. xylosus, s. saprophyticus and s. carnosus. the two latter species prevented production of enterotoxin c(1) and s. xylosus markedly reduced the amount produced. the three ...199622060592
tyramine degradation by micrococci during ripening of fermented sausage.the ability of tyramine oxidase exhibiting strains of micrococcus varians to degrade tyramine in vivo during sausage fermentation was investigated. fermented sausage was produced with a tyramine forming strain of lactobacillus curvatus which acquired a final tyramine concentration of 190ppm. the addition of either one of two strains of m. varians exhibiting a potential to oxidise tyramine decreased the amount of tyramine formed to 160 and 150ppm, respectively. no effect on growth of l. curvatus ...199822060579
effect of chitosan in meat preservation.the effect of chitosan in meat preservation, including microbiological, chemical, sensory and color qualities, was examined. in liquid medium, chitosan (0·01%) inhibited the growth of some spoilage bacteria such as bacillus subtilis ifo 3025, escherichia coli rb, pseudomonas fragi ifo 3458 and staphylococcus aureus iam 1011. at higher concentrations (0·1 and 1·0%) it inhibited the growth of the meat starter cultures, lactobacillus plantarium iam 1216, pediococcus pentosaceus iam 12296 and microc ...199422059662
halophilic characterization of starch-binding domain from kocuria varians α-amylase.the tandem starch-binding domains (kvsbd) located at carboxy-terminal region of halophilic α-amylase from moderate halophile, kocuria varians, were expressed in e. coli with amino-terminal hexa-his-tag and purified to homogeneity. the recombinant kvsbd showed binding activity to raw starch granules at low to high salt concentrations. the binding activity of kvsbd to starch was fully reversible after heat-treatment at 85°c. circular dichroism and thermal scanning experiments indicated that kvsbd ...201222020156
Isolation and characterization of aerobic culturable arsenic-resistant bacteria from surfacewater and groundwater of Rautahat District, Nepal.Arsenic (As) contamination of groundwater is a serious Environmental Health Management issue of drinking water sources especially in Terai region of Nepal. Many studies have reported that due to natural abundance of arsenic in the environment, various bacteria have developed different resistance mechanisms for arsenic compound. In this study, the culturable arsenic-resistant bacteria indigenous to surfacewater as well as groundwater from Rautahat District of Nepal were randomly isolated by stand ...201121868146
volatile and biogenic amines, microbiological counts, and bacterial amino acid decarboxylase activity throughout the salt-ripening process of anchovies (engraulis encrasicholus).chemical and microbiological parameters were studied during the industrial production of salt-ripened anchovies (engraulis encrasicholus). gradual acidification and increases in the proteolysis index and in total volatile basic nitrogen were observed. at the end of the maturing process, the values reached ph 5.55 +/- 0.03, 21.33 +/- 5.82%, and 44.06 +/- 12.47 mg/ 100 g, respectively. in the three studied anchovy batches, the biogenic amines tyramine, histamine, putrescine, cadaverine, and agmati ...200521132979
interactions between lactobacillus sakei and cnc (staphylococcus xylosus and kocuria varians) and their influence on proteolytic evaluate interactions between lactobacillus sakei and coagulase negative cocci (cnc) (staphylococcus xylosus and kocuria varians) and to investigate the influence of these interactions on their own proteolytic activity.201020875035
salt-dependent thermo-reversible α-amylase: cloning and characterization of halophilic α-amylase from moderately halophilic bacterium, kocuria varians.a moderately halophilic bacterium, kocuria varians, was found to produce active α-amylase (k. varians α-amylase (kva)). we have observed at least six different forms of α-amylase secreted by this bacterium into the culture medium. characterization of these kva forms and cloning of the corresponding gene revealed that kva comprises pre-pro-precursor form of α-amylase catalytic domain followed by the tandem repeats, which show high similarity to each other and to the starch binding domain (sbd) of ...201020871989
kocuria varians infection associated with brain abscess: a case report.kocuria, established by stackebrandt et al., previously was classified into micrococcus. only two species, k. rosea and k. kristinae are reported to be associated as pathogenic and found with catheter-related bacteremia and acute cholecystitis.201020423506
biodegradation of international jet a-1 aviation fuel by microorganisms isolated from aircraft tank and joint hydrant storage systems.microorganisms contaminating international jet a-1 aircraft fuel and fuel preserved in joint hydrant storage tank (jhst) were isolated, characterized and identified. the isolates were bacillus subtillis, bacillus megaterium, flavobacterium oderatum, sarcina flava, micrococcus varians, pseudomonas aeruginosa, bacillus licheniformis, bacillus cereus and bacillus brevis. others included candida tropicalis, candida albicans, saccharomyces estuari, saccharomyces cerevisiae, schizosaccharomyces pombe, ...200919452113
isolation and identification of atrazine-degrading bacteria from corn field soil in fars province of this study several agricultural fields with a long history of atrazine application in fars province of iran have been explored for their potential of atrazine biodegradation. after several subculturing for a period of 300 days acclimation, leads to an enhancement of atrazine biodegradation rate. a successful enrichment culture with a high capability for atrazine degradation was obtained (88%). a combination of enrichment culture technique, in a basal salt medium containing atrazine and carbon ...200719069990
combined use of starter cultures and preservatives to control production of biogenic amines and improve sensorial profile in low-acid salami.the combined effect of starter culture, nitrites, and nitrates has been studied in low-acidity salamis, typical products of northern italy. nine batches have been prepared, combining three different inoculations of starter cultures (control, lactobacillus plantarum , and lactobacillus plantarum together with kocuria varians ) with three different preservatives (control, sodium nitrate, and sodium nitrite). all of the batches showed a good fermentation process with a proper ph decrease, which was ...200818986149
staphylococcus pseudolugdunensis sp. nov., a pyrrolidonyl arylamidase/ornithine decarboxylase-positive bacterium isolated from blood cultures.twelve coagulase-negative staphylococcal isolates recovered from blood cultures with positive pyrrolidonyl arylamidase and ornithine decarboxylase reactions were identified as staphylococcus lugdunensis by the clinical microbiology laboratory. however, none of these 12 isolates were recognized by a s. lugdunensis translation elongation factor tu (tuf) gene-specific probe. under the api staph v4.0 identification system (biomérieux, durham, nc), 8 of these 12 isolates could not be identified with ...200818248933
interactions between strains of staphylococcus xylosus and kocuria varians isolated from fermented evaluate the interactions of staphylococcus xylosus on kocuria varians strains isolated from fermented meat products. methods and results: interactions were assessed in vitro by agar spot test, agar well diffusion assay and spectrophotometric assay. the growth of k. varians (five strains) alone was compared with that in the presence of growing cells of s. xylosus (50 strains) or in the presence of heat-treated or untreated supernatants of s. xylosus. sixteen strains stimulated the growth of k ...200717714408
a study of the microbial quality and organoleptic properties of ophthalmic preparations obtained from traditional medical practitioners in south western nigeria.twenty eight samples of traditional ophthalmic preparations were obtained from various traditional medical practitioners in the south western states of nigeria over a one-year period. they were examined for sterility, clarity, presence of particulate contamination and ph. all of them were found to be contaminated with bacteria to the order of 2.00 x 10(6) to 1.60 x 10(11) colony forming units/ml, with pseudomonas species and other potential pathogens such as staphylococcus aureus, yersinia enter ...200617209322
microbiological characteristics of "androlla", a spanish traditional pork sausage.counts of total aerobic mesophilic microflora, lactic acid bacteria, salt-tolerant microflora, enterobacteriaceae, enterococci, moulds and yeasts, and staphylococci, and some physico-chemical parameters (total solids, nacl and nitrate contents and ph and aw values) were determined in 20 units of "androlla", a traditional dry-fermented sausage made in the nw of spain. in general, high counts of all the investigated microbial groups were observed, with average values of 8.99 +/- 0.46 log cfu/g for ...200716943094
construction and analysis of fractional multifactorial designs to study attachment strength and transfer of listeria monocytogenes from pure or mixed biofilms after contact with a solid model food.the aim of this study was to establish which of seven factors influence the adhesion strength and hence bacterial transfer between biofilms containing listeria monocytogenes (pure and two-species biofilms) and tryptone soya agar (tsa) as a solid organic surface. the two-species biofilms were made of l. monocytogenes and one of the following species of bacteria: the nonpathogenic organisms kocuria varians, pseudomonas fluorescens, and staphylococcus sciuri and ccl 63, an unidentified gram-negativ ...200616597925
production of 5' nucleotide by using halophilic nuclease h preferentially adsorbed on flocculated cells of the halophile micrococcus varians subsp. halophilus.a bioreactor with a column of flocculated cells of the moderate halophile micrococcus varians subsp. halophilus which adsorbed the halophilic nuclease h was designed to be used in the production of 5' nucleotides from rna. a remarkable characteristic of the flocculated cells was that they preferentially adsorbed much exogenous nuclease, excluding adsorbed 5' nucleotidase. furthermore, desalting treatment of the flocculated cells in the presence of 2% mgso(4) . 7h(2)o gave rise to selective inact ...198816347767
molecular, technological and safety characterization of gram-positive catalase-positive cocci from slightly fermented sausages.the population of gram-positive catalase-positive cocci from slightly fermented sausages was characterized at species and strain level by molecular techniques and some technological and hygienic aspects were also considered. staphylococcus xylosus was the predominant species (80.8%) followed by staphylococcus warneri (8.3%), staphylococcus epidermidis (5.8%) staphylococcus carnosus (4.6%), and kocuria varians (0.4%). proteolytic activity was observed in 23% of the isolates. the species with the ...200616297478
mva1269i: a monomeric type iis restriction endonuclease from micrococcus varians with two ecori- and foki-like catalytic domains.type ii restriction endonuclease mva1269i recognizes an asymmetric dna sequence 5'-gaatgcn / -3'/5'-ng / cattc-3' and cuts top and bottom dna strands at positions, indicated by the "/" symbol. most restriction endonucleases require dimerization to cleave both strands of dna. we found that mva1269i is a monomer both in solution and upon binding of cognate dna. protein fold-recognition analysis revealed that mva1269i comprises two "pd-(d/e)xk" domains. the n-terminal domain is related to the 5'-ga ...200516223716
reclassification of strain ccm 132, previously classified as kocuria varians, as kocuria carniphila sp. nov.a gram-positive actinobacterium, previously classified as kocuria varians, was subjected to a polyphasic taxonomic study. the bacterium showed the peptidoglycan type lys-ala3 (variation a3alpha), mk-7(h2) was the major menaquinone and anteiso-c(15 : 0) and anteiso-c(17 : 0) were the major fatty acids. on the basis of the phylogenetic and phenotypic characteristics of the actinobacterium, a novel species, kocuria carniphila sp. nov. (type strain, ccm 132t=dsm 16004t), is proposed.200515653866
interactions in biofilms between listeria monocytogenes and resident microorganisms from food industry premises.twenty nine bacterial strains were grown as binary culture biofilms with listeria monocytogenes to assess their influence on the settlement of the latter on stainless steel coupons. most of the strains had been isolated from food processing plants after cleaning and disinfection and were tentatively identified by the apilab plus 3.3.3 database (biomerieux). sixteen of them decreased l. monocytogenes biofilm colony forming units (cfu) counts. three strains, bacillus sp. ccl 9 an unidentified gram ...200415541798
antimicrobial potential of immobilized lactococcus lactis subsp. lactis atcc 11454 against selected bacteria.immobilization of living cells of lactic acid bacteria could be an alternative or complementary method of immobilizing organic acids and bacteriocins and inhibit undesirable bacteria in foods. this study evaluated the inhibition potential of immobilized lactococcus lactis subsp. lactis atcc 11454 on selected bacteria by a modified method of the agar spot test. l. lactis was immobilized in calcium alginate (1 to 2%)-whey protein concentrate (0 and 1%) beads. the antimicrobial potential of immobil ...200415222547
validation of a microbiological method: the star protocol, a five-plate test, for the screening of antibiotic residues in milk.the results of an in-house laboratory validation of a microbiological method for the screening of antibiotic residues in milk are presented. the sensitivity of this five-plate test, called screening test for antibiotic residues (star), was established by the analysis of milk samples spiked with 66 antibiotics at eight different concentrations. ten different groups of antibiotics were studied: macrolides, aminoglycosides, cephalosporins, penicillins, quinolones, tetracyclines, sulphonamides, linc ...200415204543
contribution of starter cultures to the proteolytic process of a fermented non-dried whole muscle ham product.porcine longissimus dorsi muscles were cured by brine injection. curing brine containing 15% (w/v) nacl, 1.33% (w/v) glucose, 750 ppm sodium nitrite, and appropriate levels of either lactobacillus sakei lad, l. sakei lad plus kocuria varians ft4 (formally micrococcus varians), l. sakei lad plus papain and gdl (glucono-delta-lactone) plus k. varians ft4, was injected to the muscle at a pumping rate 15% w/v. the effect of these treatments on the proteolysis in the ham system was compared to a cont ...200415135960
[brain abscesses after extracranial infections of the head and neck area].the authors report on 20 immunocompetent patients with brain abscess after 12 cases of middle ear, seven tooth and a single frontal sinus infection. the clinical aspects, hematochemical and microbiological data, the role of imaging diagnostics (ct, mr) and the type of treatment are analysed. neurosurgery was performed on 17 patients (85%), eight of whom subsequently underwent evacuation of the primary source of infection (four mastoidectomies, two timpanoplasties, two tooth extractions). mastoid ...200314523535
infection of a ventriculoatrial shunt with phenotypically variable staphylococcus epidermidis masquerading as polymicrobial bacteremia due to various coagulase-negative staphylococci and kocuria varians.the diagnosis of bloodstream infection with coagulase-negative staphylococci is frequently based on the isolation of the same organism from more than one blood culture. phenotypic variation is a common characteristic of pathogenic strains of staphylococcus epidermidis which may affect species identification by the microbiology laboratory. we describe a patient with a new onset of nephritis and gram-positive bacteremia. gram-positive cocci grew in multiple blood cultures and were identified by th ...200312791862
kocuria polaris sp. nov., an orange-pigmented psychrophilic bacterium isolated from an antarctic cyanobacterial mat sample.strain cms 76ort, an orange-pigmented bacterium, was isolated from a cyanobacterial mat sample from a pond located in mcmurdo dry valley, antarctica. on the basis of chemotaxonomic and phylogenetic properties, strain cms 76ort was identified as a member of the genus kocuria. it exhibited a 16s rdna similarity of 99.8% and dna-dna similarity of 71% with kocuria rosea (atcc 186t). phenotypic traits confirmed that strain cms 78ort and k. rosea were well differentiated. furthermore, strain cms 76ort ...200312656171
[occurrence of bacteria in the mouth from genera of micrococcus, kocuria, nesterenkonia, kytococcus and dermacoccus].the aim of the study was to assess the prevalence of different bacteria in the oral cavity. the bacteria were present in the oral cavities of 73 (48.7%) of 150 individuals. nesterenkonia halobia, the most frequently isolated species, was found in 20 (27%) individuals, micrococcus luteus in 16 (22%), kocuria kristinae in 12 (16%), kocuria varians in 10 (14%), dermacoccus sedentarius in 9 (12%), micrococcus lylae in 8 (11%), and kytococcus nishinomiyaensis in 3 (4%). mean counts of these microorga ...200212185681
degradation of organochlorine pesticides by meat starter in liquid media and fermented sausage.the effect of meat starter on the degradation of ddt and lindane was investigated. the insignificant role of lactobacillus plantarum in degrading p,p'-ddt and lindane presented in tryptone soya broth (tsb) and mineral salt medium (msm) with or without 120 ppm nitrite was observed.the degradation of ddt and lindane by micrococcus varians in tsb and msm with or without nitrite were studied. the results indicated that ddt or lindane were degraded during the incubation period. the reduction in ddt a ...200211731034
delayed infections after posterior tsrh spinal instrumentation for idiopathic scoliosis: determine the incidence of delayed infections in idiopathic scoliosis treated with tsrh instrumentation, proper wound management after instrumentation removal, and whether the previously identified bacterial trend remains consistent.200111547197
the application of a fermented food ingredient containing 'variacin', a novel antimicrobial produced by kocuria varians, to control the growth of bacillus cereus in chilled dairy products.the feasibility of applying variacin, a lantibiotic produced by kocuria varians in the form of a spray-dried fermented ingredient to control the growth of psychrotrophic bacillus cereus strains in chilled dairy foods, was evaluated.200111155129
a 23s rdna-targeted polymerase chain reaction-based system for detection of staphylococcus aureus in meat starter cultures and dairy products.a polymerase chain reaction-based system for detection of staphylococcus aureus was developed. the system consisted of the following components: (i) selective enrichment, (ii) dna isolation, (iii) amplification of dna with primers targeted against the 23s rrna gene, and (iv) evaluation of the specificity of the polymerase chain reaction by southern hybridization and nested polymerase chain reaction. the method achieved a high degree of sensitivity and unambiguity as required for the detection of ...199910528718
antimicrobial activity of monascus pilosus ifo 4520 against contaminant of koji.antimicrobial activity of monascus pilosus ifo 4520 was examined to prevent contamination during beni-koji making in the open air. the antibacterial effect of the beni-koji prepared with this strain occured with 30 mg/ml of beni-koji extract in combination with 0.5% lactic acid against two contaminants of koji, micrococcus varians and bacillus subtilis. there were two compounds, antibacterial and antiyeast substances, in the beni-koji extract. these results suggest a possibility of inhibiting th ...199910501009
kocuria palustris sp. nov. and kocuria rhizophila sp. nov., isolated from the rhizoplane of the narrow-leaved cattail (typha angustifolia).two gram-positive, aerobic spherical actinobacteria were isolated from the rhizoplane of narrow-leaved cattail (typha angustifolia) collected from a floating mat in the soroksár tributary of the danube river, hungary. sequence comparisons of the 16s rdna indicated these isolates to be phylogenetic neighbours of members of the genus kocuria, family micrococcaceae, in which they represent two novel lineages. the phylogenetic distinctness of the two organisms ta68t and taga27t was supported by dna- ...199910028258
histamine and tyramine degradation by food fermenting microorganisms.microorganisms suitable for food fermentation were examined with regard to their potential to degrade histamine and tyramine. out of 64 lactic acid bacteria evaluated in this study, 27 degraded histamine and one tyramine, respectively, with low activity. among 32 strains of brevibacterium linens and coryneform bacteria, 21 exhibited histamine and tyramine oxidase activity. none of 20 strains of staphylococcus carnosus tested degraded histamine or tyramine. one strain out of nine strains of geotr ...19989562873
amino acid decarboxylase capability of microorganisms isolated in spanish fermented meat products.enterobacteria, lactic acid bacteria (lab) and gram-positive cocci were isolated from spanish meat products. the most frequent species in the meat products studied were identified as lactobacillus sake, lactobacillus plantarum and lactobacillus curvatus from de man-rogosa-sharpe agar; staphylococcus xylosus, staphylococcus saprophyticus and micrococcus varians from mannitol salt phenol-red agar; and hafnia alvei, escherichia coli, pseudomonas fluorescens, enterobacter amnigenes and enterobacter ...19989553801
activity of bacterial phosphomonoesterases in batch culture.the phosphomonoesterases catalyse the hydrolysis of primary esters of phosphoric acid which help the bacteria to survive in phosphate stressed environment. ninety-five bacterial isolates were obtained from domestic sewage and industrial effluents of gelatine and soap factories at jabalpur on a medium enriched with phosphate and were screened for phosphatase production. the phosphatase producers were tentatively identified as escherichia coli, vibrio vulnificus, aeromonas hydrophila, staphylococc ...19989536656
variacin, a new lanthionine-containing bacteriocin produced by micrococcus varians: comparison to lacticin 481 of lactococcus lactis.a new lanthionine-containing bacteriocin, variacin, displaying a broad host range of inhibition against gram-positive food spoilage bacteria, has been identified from two strains of micrococcus varians isolated from meat fermentations. the new bacteriocin was purified, and its amino-terminal end and total amino acid composition were determined. the structural gene was isolated and analyzed. variacin is resistant to heat and ph conditions from 2 to 10. its primary sequence shows significant homol ...19968633879
micrococci demonstrate a phosphatase activity which is repressed by phosphates and which can be differentiated from that of staphylococci.the phosphatase activities of 114 micrococcal strains belonging to seven different species and of an additional 150 unspeciated micrococcal strains were evaluated on solid media at various phs containing or not containing phosphates. in the presence of phosphates, only nine strains (five unspeciated strains, one micrococcus luteus strain, and three micrococcus varians strains) yielded a positive reaction on plates at ph 8. in media (at ph 8) deprived of phosphates, in contrast, all but 15 strain ...19938240961
identification of psychrotrophic micrococcaceae spp. isolated from fresh beef stored under carbon dioxide or vacuum.seventy-four gram-positive, catalase-positive coccal strains were isolated from fresh beef stored under carbon dioxide (< 500 ppm o2) or vacuum for up to 15 weeks at 0, 2 or 4 degrees c. isolates were identified using biochemical tests listed in several published protocols and the api staph-ident system. no isolates were identified as staphylococcus aureus. twenty-nine isolates were identified as staphylococcus saprophyticus (five distinct groups), 24 isolates were identified as staphylococcus g ...19947848779
delayed infections following posterior spinal instrumentation for the treatment of idiopathic scoliosis.ten patients who had been managed with posterior spinal arthrodesis and texas scottish rite hospital instrumentation because of idiopathic scoliosis had a delayed deep wound infection at an average of twenty-five months after the operation. the signs of infection included spontaneous drainage in eight patients and fluctuance in two patients. in addition, six patients--including five of the eight who had drainage--had mild pain in the back. the average erythrocyte sedimentation rate was thirty-ni ...19957713968
taxonomic dissection of the genus micrococcus: kocuria gen. nov., nesterenkonia gen. nov., kytococcus gen. nov., dermacoccus gen. nov., and micrococcus cohn 1872 gen. emend.the results of a phylogenetic and chemotaxonomic analysis of the genus micrococcus indicated that it is significantly heterogeneous. except for micrococcus lylae, no species groups phylogenetically with the type species of the genus, micrococcus luteus. the other members of the genus form three separate phylogenetic lines which on the basis of chemotaxonomic properties can be assigned to four genera. these genera are the genus kocuria gen. nov. for micrococcus roseus, micrococcus varians, and mi ...19957547287
biosynthesis of teichoic acid in micrococcus varians atcc 29750. characterization of a further lipid intermediate. 19807358174
valine production from hydrocarbon by micrococcus varians.a bacterium isolated from assam (india) soil was found to accumulate l-valine in the growth medium and was identified as micrococcus varians. the strain grew and accumulated valine in a purely synthetic medium, but supplementation with either casamino acids or yeast extract or with both, improved the yield. the entire fermentation period could be divided into a growth phase and a production (phase which could be prolonged by adjustment of ph to neutral range. among the different hydrocarbon and ...19827141324
viability of micrococci and lactobacilli upon freezing and freeze-drying in the presence of different cryoprotectants.studies were conducted on the viability of micrococcus varians strain m95 and lactobacillus plantarum strain l4 upon freezing and freeze-drying using five cryoprotectants (sucrose, lactose, sodium glutamate, peptone, dry nonfat milk) singly or in combinations with gelatin, glutamic acid, and sodium acetate. the number of survivals was determined immediately after treatment and after storage at room temperature or refrigeration temperatures, under vacuum or in air. dry nonfat milk and peptone int ...19836884073
mutation modifying the serine pathway in methylotrophic bacteria.methylotrophic bacteria, gram-positive, with the serine pathway, were shown to have their growth inhibited by 0.5% glycine. the effects of this amino acid on individual enzyme activities were studied in wild and mutant strains of micrococcus varians and bacillus licheniformis. the enzymes studied were glycerate dehydrogenase (ec, isocitrate lyase (ec, serine hydroxymethyltransferase (ec and glycine--oxaloacetate aminotransferase (ec the last-named enzyme wa ...19816792008
treatment of csf shunt infections with intrashunt plus oral antibiotic therapy.infections of 12 cerebrospinal fluid (csf) shunts in 11 children were treated with oral systemic antibiotic therapy plus daily intrashunt injections of antibiotics. eight patients were infected with staphylococcus epidermidis (four patients) or staphylococcus aureus (four patients), and were treated with intrashunt vancomycin, plus oral trimethoprim/sulfamethoxazole (t/s), plus oral rifampin. one of these eight patients was later changed to a course of intrashunt cephapirin and oral cephalexin p ...19846607326
lysine production from hydrocarbon by micrococcus varians 2fa.a bacterium isolated from assam (india) soil was found to accumulale l-lysine from hydrocarbon and was identified as a strain of micrococcus varians. the strain is able to grow and accumulate lysine in a purely synthetic medium though supplementation of the synthetic medium with casamino acids significantly improves the yield. the yield of l-lysine under optimal conditions was found to be 2.6 g x 1(-1) of the compound isolated in crystalline form.19836196944
application of halophilic nuclease h of micrococcus varians subsp. halophilus to commercial production of flavoring agent 5'-gmp.rna was degraded at 60 degrees c for 24 h by halophilic nuclease h in supernatants from broth cultures of micrococcus varians subsp. halophilus containing 12% nacl. since contaminating 5'-nucleotidase exhibited almost no activity under these conditions, the 5'-gmp formed could be recovered from the reaction mixture, and the yield was 805 mg from 5 g of rna.19826184020
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the penetration of cephalothin into synovial and other body fluids. 20164973975
hospital matrons--unlimited hours--appeal judgment of the industrial court. 20164973939
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[secretory function of the stomach and significance of the acid-peptic factor in peptic ulcer (literature review)]. 20164943938
the "germ of laziness" in the south, 1900-1915: charles wardell stiles and the progressive paradox. 20164932199
halophilic nuclease from a moderately halophilic micrococcus varians.the moderately halophilic bacterium micrococcus varians, isolated from soy sauce mash, produced extracellular nuclease when cultivated aerobically in media containing 1 to 4 m nacl or kcl. the enzyme, purified to an electrophoretically homogeneous state, had both ribonuclease and deoxyribonuclease activities. the nuclease had maximal activity in the presence of 2.9 m nacl or 2.1 m kcl at 40 c. the enzymatic activity was lost by dialysis against low-salt buffer, whereas when the inactivated enzym ...19744852218
[post-infarct dissecting hematoma of the left ventricle as a cause of acute subvalvular mitral incompetence]. 20164820484
a death at home. 20164816608
serum enzymes and prognosis in cerebral infarction. 20164778616
remedy for unsuccessful urethrostomy. 19734692301
[effects of germine monoacetate on muscle contraction and respiration]. 19724674922
cloverleaf skull. 20154669244
morrill wyman and the aspiration of acute pleural effusions, 1850. a letter from new england. 20154561702
[pathogen content of air in clean rooms--origin, methods of determination and definition]. 20154556743
dosimetry at interfaces. theoretical analysis and measurements by means of thermoluminescent lif at plane interfaces between a low z-material and al, cu, sn or pb irradiated with 100 to 200 kv roentgen radiation. 20144519462
dosimetry at interfaces. theoretical analysis and measurements by means of thermoluminescent lif at plane interfaces between a low z-material and al, cu, sn or pb irradiated with 100 to 200 kv roentgen radiation. 20144519462
[use of the "delphi" method in predicting the overall morbidity of the population]. 20154490520
hexachlorophene intoxication: characterization of myelin and myelin related fractions in the rat during early postnatal development. 19744435077
proceedings: 'bonneau report raises question of health research in quebec'. 20154415217
partial purification and some properties of guinea pig kidney 17 -hydroxy-c 19 steroid dehydrogenase. 19724402861
[pulmonary findings in collagen diseases with special reference to thoracic radiography]. 20154397695
[hydroxylation of progesterone by rabbit liver microsomes. changes in metabolite pattern after phenobarbital treatment of the animals]. 19704394453
[luminescent method of diagnosing poultry diseases]. 19714331609
[luminescent method of diagnosing poultry diseases]. 19714331609
histochemical study on alkaline phosphatase activity in human neoplasms. 19664290958
histochemical study on alkaline phosphatase activity in human neoplasms. 19664290958
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