PMID(sorted descending)
toll-like receptor 4-deficient mice have reduced bone destruction following mixed anaerobic infection.c3h/hej mice have an impaired ability to respond to lipopolysaccharide (lps) due to a mutation in the gene that encodes toll-like receptor 4 (tlr4). the effect of tlr4 deficiency on host responses to endodontic infections is unknown. in the present study, we compared periapical bone destruction, sepsis, and inflammatory cytokine production in lps-hyporesponsive c3h/hej and wild-type control c3h/heouj mice. the mandibular first molars of both strains were subjected to pulpal exposure and infectio ...200010899873
in vitro activities of mk-826 (l-749,345) against 363 strains of anaerobic bacteria.the activity of mk-826 was compared to the activities of cefoxitin, ceftriaxone, imipenem, and meropenem against 363 gram-negative and gram-positive anaerobes by using nccls procedures. at least 98% of the strains were susceptible to the carbapenems. all strains of clostridium perfringens, fusobacterium nucleatum, peptostreptococcus, and sutterella wadsworthensis were susceptible to all agents tested.200010898709
proteolysis and utilization of albumin by enrichment cultures of subgingival microbiota.subgingival dental plaque consists mainly of microorganisms that derive their energy from amino acid fermentation. their nutrient requirements are met by the subgingival proteolytic system, which includes proteases from microorganism and inflammatory cells, and substrate proteins from sulcus exudate, including albumin. to determine the selective effect of individual proteins on microbiota, we used albumin as the main substrate for growth. eight subgingval plaque samples from untreated periodonta ...199910895689
[cerebral abscess in an immunocompetent patient]. 200010893748
the periodontal abscess (i). clinical and microbiological findings.little information is available regarding the diagnosis and microbiology of periodontal abscesses. the aim of this descriptive clinical and microbiological study was to provide more information in order to help in the characterisation of the periodontal abscess associated to periodontitis.200010883867
in vitro activity of telithromycin (hmr 3647) and seven other antimicrobial agents against anaerobic bacteria.we assessed the in vitro activity of telithromycin (hmr 3647) and seven other antimicrobials against 292 strains of obligately anaerobic bacteria. mics were determined with the microdilution technique and wilkins-chalgren broth according to din 58940-83. mic50/mic90s (mg/l) for telithromycin were 4/4 for bacteroides fragilis, bacteroides ovatus and bacteroides thetaiotaomicron, 2/4 for fusobacterium spp. and bilophila wadsworthia, 2/2 for bacteroides caccae, 1/4 for bacteroides vulgatus, 0.25/4 ...200010882699
ulcus vulvae acute vulvar ulcer was diagnosed based on a case history of a 17-year-old girl with genital ulcers. no relevant causative factors could be established. the gangrenous form of the disease, cutaneous anergy, and the cultured anaerobic pathogens suggested the possibility of an infectious pathogenesis.200010879308
features of severe periodontal disease in a teenager with chédiak-higashi syndrome.chédiak-higashi syndrome (c-hs) is a rare congenital disease characterized by defective neutrophil function with abnormal lysosomal inclusions, neutropenia, and reduced chemotaxis. the complete syndrome includes oculocutaneous albinism with photophobia, neurologic features, recurrent infections, and enterocolitis.200010872965
early microbial colonization of permucosal implants in edentulous edentulous patients, the microbial colonization of permucosal implants of sintered hydroxyapatite was studied. samples were taken from mucosa and dentures before insertion of implants and from supra- and subgingival sites two to 10 weeks after insertion. in total, five patients and 10 implants with clinically healthy peri-implant tissues were studied. the samples were investigated by dark-field microscopy and anaerobic culture. the supragingival plaque of the implants was dominated by gram-po ...198710872401
[the range of antagonistic effects of lactobacillus bacterial strains on etiologic agents of bacterial vaginosis].bacterial vaginosis is caused by uncontrolled sequential overgrowth of some anaerobic bacteria: gardnerella vaginalis, prevotella bivia, bacteroides spp., peptostreptococcus spp., mobiluncus sp. usually occurring in stable numbers in the bacterial flora of healthy women. on the other hand, different species of bacteria belonging to the genus lactobacillus, most frequently l. plantarum, l. rhamnosus and l. acidophilus, form a group of aerobic bacteria dominating in the same environment. the diver ...199910865430
microbiology of healthy and diseased determine the qualitative and quantitative microbiology of core adenoid tissue obtained from four groups of 15 children each, with recurrent otitis media (rom), recurrent adenotonsillitis (rat), obstructive adenoid hypertrophy (oah), and occlusion or speech abnormalities (controls).200010852520
a prospective, randomized, clinical study to compare the clinical safety, effectiveness, and cost of oral ofloxacin/clindamycin vs intravenous clindamycin/gentamicin for the treatment of postpartum endomyometritis.objective: the primary objective of this prospective, randomized, clinical study was to compare the safety, clinical and microbiologic efficacy, and cost of oral ofloxacin in combination with clindamycin vs intravenous (iv) clindamycin/gentamicin in the early empiric treatment for hospitalized patients with mild to moderate postpartum endomyometritis. the secondary objective is to reduce total hospital and patient treatment cost. postpartum endomyometritis is a major cause of infectious morbidit ...199810838268
review of the in vitro activity of gemifloxacin against gram-positive and gram-negative anaerobic pathogens.published reports on the in vitro activity of gemifloxacin mesylate (sb 265805), a new fluoronaphthyridone, against anaerobic pathogens are reviewed here. the studies used a variety of media, inocula and antimicrobial agents. using a proposed breakpoint of 0.5 mg/l, these studies showed that gemifloxacin had generally higher potency against gram-positive anaerobes (clostridium perfringens, all peptostreptococcus spp.) and fusobacteria (fusobacterium nucleatum, fusobacterium necrophorum) and mode ...200010824034
induction of human immunodeficiency virus type 1 expression by anaerobes associated with bacterial vaginosis.bacterial vaginosis (bv) is a common disorder characterized by increased levels of anaerobic bacteria in the genital tract. bv has been associated with an increased rate of sexual transmission of human immunodeficiency virus (hiv). the effects of bv-associated anaerobic bacteria on hiv expression in monocytoid cells and t cells were examined. peptostreptococcus asaccharolyticus and prevotella bivia stimulated hiv expression in monocytoid cells, whereas bacteroides ureolyticus, peptostreptococcus ...200010823756
strategy for 3-d computed tomography diagnosis and treatment of small pulmonary arteriovenous a patient with left hemianopsia, computed tomography (ct) and magnetic resonance imaging revealed an intracranial space-occupying lesion in the right occipital region. a cerebral abscess was removed at craniotomy. enhanced pulmonary ct showed a small coin lesion in the peripheral lingula. and at 3-d ct two nidi with feeding arteries and draining veins were seen, indicating pulmonary arteriovenous fistula. lingular segmentectomy was performed.200010816068
[what is your diagnosis? bilateral abscess forming pneumonia]. 200010815462
microbiology of infected poison ivy dermatitis.we report the aerobic and anaerobic microbiology of secondarily infected poison ivy dermatitis. the study involved retrospective review of clinical and microbiology laboratory records of patients with secondarily infected poison ivy lesions. bacterial growth was noted in 33 specimens. aerobic or facultative anaerobic bacteria only were present in 18 (55%) patients, anaerobic bacteria only in seven (21%), and mixed anaerobic-aerobic bacteria in eight (24%). forty-five isolates were recovered (1.4 ...200010809852
in vitro inhibition of commercial douche products against vaginal microflora.recently, vaginal douching has been associated with many health risks in women. the aim of this study was to analyze the effect of commercial douche products against various vaginal microorganisms, including lactobacilli. seven commercial douches were tested against eight lactobacillus clinical isolates and three type strains from the american type culture collection. bv-associated bacteria included six strains of five genera: gardnerella, mobiluncus, mycoplasma, peptostreptococcus, and ureaplas ...200010805365
[otogenic cerebral abscess: a persistent problem].a young man with otitis media and cholesteatoma of the left ear developed secondary cerebral abscess. the clinical debut was non-specific, with headache, mild fever, and mild persistent otalgia in spite of early antibiotic treatment. studies revealed a cerebral abscess, so ent surgery in collaboration with the neurosurgery department was decided. this case illustrates that clinical manifestations in such cases can be mild and highlights the need to exclude this type of serious pathology.200010804119
linezolid.linezolid is an oxazolidinone antibacterial agent that acts by inhibiting the initiation of bacterial protein synthesis. cross-resistance between linezolid and other inhibitors of protein synthesis has not been demonstrated. linezolid has a wide spectrum of activity against gram-positive organisms including methicillin-resistant staphylococci, penicillin-resistant pneumococci and vancomycin-resistant enterococcus faecalis and e. faecium. anerobes such as clostridium spp., peptostreptococcus spp. ...200010804037
a breakdown of symmetry in the folding transition state of protein l.the 62 residue igg binding domain of protein l consists of a central alpha-helix packed on a four-stranded beta-sheet formed by n and c-terminal beta-hairpins. the overall topology of the protein is quite symmetric: the beta-hairpins have similar lengths and make very similar interactions with the central helix. characterization of the effects of 70 point mutations distributed throughout the protein on the kinetics of folding and unfolding reveals that this symmetry is completely broken during f ...200010801362
translocation of indigenous microflora in an experimental model of sepsis.translocation of viable bacteria from gut to bloodstream and other sterile body sites during shock has been demonstrated in several experimental and clinical studies. the factors causing translocation and its incidence at different stages of shock are not known. the aim of the study was to evaluate the importance of several factors causing translocation of indigenous microflora in an experimental model of septic shock based on intraperitoneal escherichia coli sepsis in rats. counts of inoculated ...200010798556
synergic activity, for anaerobes, of trovafloxacin with clindamycin or metronidazole: chequerboard and time-kill methods.chequerboard titrations were used to test the activity of trovafloxacin, alone and in combination with clindamycin or metronidazole, against 156 gram-positive or gram-negative anaerobes, including 47 bacteroides fragilis group, 36 prevotella spp., 26 fusobacteria, 21 peptostreptococci and 26 clostridia. mic50/mic90 values (mg/l) of each drug alone against all 156 strains were: trovafloxacin, 0.5/1; clindamycin, 0.25/2; metronidazole, 1/2. synergy (fic indices </= 0.5) was seen in two strains wit ...200010797085
comparative activity of moxifloxacin in vitro against obligately anaerobic bacteria.the antimicrobial activity of moxifloxacin and seven other antibiotics (four of them quinolones) against 292 strains of obligately anaerobic bacteria was assessed employing a broth microdilution technique performed in wilkens-chalgren broth. mic50/mic90 values (mg/l) for moxifloxacin were as follows: bacteroides fragilis (n = 62) 0.25/2, bacteroides ovatus (n = 70) 1/4, bacteroides vulgatus (n = 29) 0.25/1, bacteroides thetaiotaomicron (n = 17) 2/2, bacteroides caccae (n = 11) 1/2, prevotella sp ...200010795599
dynamics of nasopharyngitis in children.our goal was to characterize the dynamics and bacterial interaction of the aerobic and anaerobic flora of nasal discharge of children at different stages of uncomplicated nasopharyngitis. methods and patients: serial semiquantitative nasopharyngeal (np) and quantitative nasal discharge (nd) cultures were taken every 3 to 5 days from 20 children in whom purulent discharge eventually developed (group 1), and a single culture was obtained from a group of 20 who had only clear discharge (group 2).200010793349
profile of subgingival microbiota in children with primary dentition.eruption of primary teeth has a great influence on the oral environment by providing suitable niches for bacterial colonization. the aim of the study was to investigate the composition of the subgingival microbiota of primary incisors, canines and molars in 40 systemically healthy children aged 4-5 yr, chosen randomly. subgingival plaque samples were taken from the mesiobuccal sites of primary incisors (61, 81), canines (53, 73) and molars (64, 84). the samples were cultured for bacterial isolat ...200010791707
an experimental mouse model to study the pathogenicity of prevotella bivia and investigations of possible virulence.induction of subcutaneous abscesses in mice was used to study the pathogenicity of prevotella bivia both in mono-infection and in mixed cultures with escherichia coli and peptostreptococcus spp. virulence factors such as coaggregation and aggregate formation of cells, haemagglutination activity and tolerance to serum bactericidal activity were investigated for their possible role in p bivia pathogenicity. monocultures of p bivia, e coli and peptostreptococcus spp did not induce subcutaneous absc ...200010786446
aerobic and anaerobic microbiology of wound infection following spinal fusion in study the aerobic and anaerobic microbiology of wound infections following spinal fusion in children.200010765134
[the clinical picture, treatment and prognosis of meningitis due to anaerobic and nonfermentative bacteria].the study of incidence, clinical manifestations and prognosis of meningitis with anaerobic and non-fermentative bacteria.199910756944
[in vitro activities of levofloxacin and other antibiotics against fresh clinical isolates].in this study, the in vitro activity of levofloxacin (lvfx) against 1,020 fresh bacterial clinical isolates was compared with the activities of a range of ofloxacin, ciprofloxacin (cpfx), ampicillin (abpc), cefaclor, cefpodoxime, methicillin and benzylpenicillin. the clinical isolates except vibrio cholerae were collected in japan during 1998 from patients with infectious diseases. mics were determined using the agar dilution method according to the recommendations by the japan society of chemot ...199910746192
expression of a heterologous glutamate dehydrogenase gene in lactococcus lactis highly improves the conversion of amino acids to aroma compounds.the first step of amino acid degradation in lactococci is a transamination, which requires an alpha-keto acid as the amino group acceptor. we have previously shown that the level of available alpha-keto acid in semihard cheese is the first limiting factor for conversion of amino acids to aroma compounds, since aroma formation is greatly enhanced by adding alpha-ketoglutarate to cheese curd. in this study we introduced a heterologous catabolic glutamate dehydrogenase (gdh) gene into lactococcus l ...200010742211
crystallization and preliminary x-ray diffraction studies of mutants of b1 igg-binding domain of protein l from peptostreptococcus magnus.the small 62-residue igg-binding domain b1 of protein l from peptostreptococcus magnus (ppl-b1) has proven to be a simple system for the study of the thermodynamics and kinetics of protein folding. x-ray diffraction studies have been initiated in order to determine how the thermostability, folding and unfolding rates of a series of point mutations spanning ppl-b1 correlate with the high-resolution structures. to this end, a tryptophan-containing variant of ppl-b1 (herein known as wild type) and ...200010739935
a case of paraspinal abscess and diskitis due to peptostreptococcus micros. 200010722468
peptostreptococcus micros smooth and rough genotypes in periodontitis and gingivitis.two genotypes can be distinguished within the species peptostreptococcus micros: a smooth (sm) and a rough (rg) type. to date no systematic study has been performed on the prevalence and proportion of both types in untreated periodontitis patients and subjects without destructive periodontal disease. therefore, the present study was performed to investigate: 1) the relative importance of the sm and the rg genotype of p micros in periodontitis and gingivitis; 2) the correlation between smoking an ...200010711611
lack of antimicrobial effect on periodontopathic bacteria by ultrasonic and sonic scalers in vitro.the purpose of this study was to assess the antimicrobial effects of a sonic and ultrasonic scaler generally used for subgingival scaling on gram-negative and gram-positive periodontopathic bacteria.200010703657
[the dynamics of the colonization by the microbial flora of the mouth of the different materials used for dental prosthetics].colonization of materials used for making dentures with oral microflora was studied in 12 patients with chronic generalized medium severe periodontitis during remission. time course of dentures colonization with representatives of oral microflora (s. sanguis, peptostreptococcus sp., prevotella oralis) and with bacteria pathogenic for the periodontium (prevotella melaninogenica, a. naeslundii, fusobacterium sp.) depends on the material from which the dentures are made. minimal colonization of bac ...200010693351
bacterial interference in the nasopharynx and nasal cavity of sinusitis prone and non-sinusitis prone children.the aim of this study was to compare the frequency of recovery of potential pathogens and aerobic and anaerobic interfering bacteria in the nasopharynx and nasal cavity of sinusitis prone (sp) children, with their recovery in non-sinusitis prone (n-sp) children. nasopharyngeal and nasal cultures were taken from 20 sp and 20 n-sp children. potential pathogens and aerobic and anaerobic bacteria with interfering capabilities against these micro-organisms were identified. twenty-one potential pathog ...199910687943
postantibiotic effects and bactericidal activities of clarithromycin-14-hydroxy-clarithromycin, versus those of amoxicillin-clavulanate, against anaerobes.the bactericidal activities and postantibiotic effects (pae) of clarithromycin-14-hydroxy-clarithromycin and amoxicillin-clavulanate against bacteroides fragilis and peptostreptococcus anaerobius were determined. a concentration of twice the mic resulted in bactericidal activity against four of four and three of four organisms at 24 h with clarithromycin-14-hydroxy-clarithromycin and amoxicillin-clavulanate, respectively. the pae of clarithromycin-14-hydroxy-clarithromycin was 1.44 to 3.20 h, co ...200010681358
aerobic and anaerobic bacteriology of concurrent chronic otitis media with effusion and chronic sinusitis in correlate the aerobic and anaerobic microbiologic findings of concurrent chronic otitis media with effusion and chronic maxillary sinusitis.200010680868
susceptibility of anaerobic bacteria in auckland: determine the antimicrobial susceptibility of local anaerobic bacteria.199910678225
past administration of beta-lactam antibiotics and increase in the emergence of beta-lactamase-producing bacteria in patients with orofacial odontogenic infections.the purpose of this study was to determine the current status of beta-lactamase-producing bacteria in orofacial odontogenic infections.200010673654
in vitro and in vivo antibacterial activities of biapenem in the fields of obstetrics and gynecology.biapenem is a new injectable carbapenem antibiotic which has favorable pharmacokinetic properties, and is stable to hydrolysis by dehydropeptidase i. biapenem inhibited more than 90% of clinical isolates of streptococcus agalactiae, escherichia coli, peptostreptococcus magnus, bacteroides fragilis and prevotella bivia at the concentration of 3.13 mg/l. the mic(90) of biapenem against pseudomonas aeruginosa was lower than that of panipenem, equivalent to that of imipenem, and greater than that of ...200010671758
evaluation of the oxyrase oxyplate anaerobe incubation system.the oxyrase oxyplate anaerobe incubation system was evaluated for its ability to support the growth of clinically significant anaerobic bacteria previously identified by the anaerobe reference laboratory at the centers for disease control and prevention. the results were compared with those obtained with conventional anaerobe blood agar plates incubated in an anaerobe chamber. we tested 251 anaerobic bacterial strains. plates were read at 24, 48, and 72 h; growth was scored by a numerical coding ...200010655335
quinupristin/dalfopristin: a review of its use in the management of serious gram-positive infections.quinupristin/dalfopristin is the first parenteral streptogramin antibacterial agent, and is a 30:70 (w/w) ratio of 2 semisynthetic pristinamycin derivatives. the combination has inhibitory activity against a broad range of gram-positive bacteria including methicillin-resistant staphylococci, vancomycin-resistant enterococcus faecium (vref), drug-resistant streptococcus pneumoniae, other streptococci, clostridium perfringens and peptostreptococcus spp. the combination also has good activity again ...199910651391
robustness of protein folding kinetics to surface hydrophobic substitutions.we use both combinatorial and site-directed mutagenesis to explore the consequences of surface hydrophobic substitutions for the folding of two small single domain proteins, the src sh3 domain, and the igg binding domain of peptostreptococcal protein l. we find that in almost every case, destabilizing surface hydrophobic substitutions have much larger effects on the rate of unfolding than on the rate of folding, suggesting that nonnative hydrophobic interactions do not significantly interfere wi ...199910631990
[results of treatment of deep neck abscesses and phlegmons].neck, as a structure very closely connected with oral cavity and pharynx, with great number of lymph nodes, (about 2, 3 of all are in the neck), is sometimes a localization of purulent inflammatory process but its incidence is not so high as the incidence of inflammations of surrounding organs and tissue. deep neck abscesses are localized under the upper fascial layer. they have a serious clinical picture which could be further complicated if inflammation spreads on vessels or neck organs. if th ...199910624393
peptostreptococcus micros coaggregates with fusobacterium nucleatum and non-encapsulated porphyromonas gingivalis.coaggregation is one of the potential colonization strategies of oral microorganisms, often involving fimbrial structures in the interactions. in this study, the coaggregation characteristics of the rough and smooth genotypes of the periodontal pathogen peptostreptococcus micros were compared to investigate the role of the fibril-like structures of the rough genotype in coaggregation. of the 11 oral species tested, only fusobacterium nucleatum strains and non-encapsulated porphyromonas gingivali ...200010612731
misleading diagnosis of retroperitoneal actinomycosis.a 34-year-old woman presented with a left-sided suprarenal space-occupying lesion on sonography. culture of material obtained during sonographic-guided puncture of the retroperitoneal lesion yielded a mixed flora of actinomyces and peptostreptococcus. initially, a misleading diagnosis of an adrenal pheochromocytoma was initiated by highly positive metaiodobenzylguanidine scintigraphy after chemical chemistry vanillylmandelic acid (vma) test showed elevated values for adrenaline and its derivativ ...199910602965
[acute infectious mastoid complications in infancy].the incidence of acute mastoiditis accounts for 0.004% of acute middle otitis. nowadays there is a decrease of number of mastoiditis and its complications, but from time to time are seen outbreaks without logical explanation of facts. we report 4 clinical cases of acute mastoiditis complicated with subperiosteal abscess, in children between 8 and 23 month old, seen at our department during the last 3 years, three of them diagnosed in a 4-month term.199910568306
microbiology of subphrenic abscesses: a 14-year experience.the objective of the review was to study the aerobic and anaerobic microbiology of subphrenic abscesses in relation with predisposing conditions. a retrospective review of clinical and laboratory data of 52 patients treated between 1974 and 1988 was conducted. forty-three (83%) patients developed the abscesses after an operative procedure. these included 11 patients after colonic, 9 patients after gastric or duodenal, 7 patients after abdominal trauma, 7 patients after biliary, and 6 patients af ...199910551755
minimal debridement in the treatment of fournier's gangrene.scrotal and perineal gangrene of the male genitalia was first described by fournier in 1883. treatment of the disease remains surgical despite the availability of modern antibiotics. many authors advocate a wide debridement of all tissue superficial to the involved fascial layers. we have reviewed our experience of using wide and minimal debridement as the surgical technique of choice. in our retrospective study, nine patients were diagnosed and treated over a 2-year period for fournier's gangre ...199910551751
in vitro utilization of amylopectin and high-amylose maize (amylomaize) starch granules by human colonic has been well established that a certain amount of ingested starch can escape digestion in the human small intestine and consequently enters the large intestine, where it may serve as a carbon source for bacterial fermentation. thirty-eight types of human colonic bacteria were screened for their capacity to utilize soluble starch, gelatinized amylopectin maize starch, and high-amylose maize starch granules by measuring the clear zones on starch agar plates. the six cultures which produced cle ...199910543795
activities of telithromycin (hmr 3647, ru 66647) compared to those of erythromycin, azithromycin, clarithromycin, roxithromycin, and other antimicrobial agents against unusual anaerobes.the comparative activity of telithromycin (hmr 3647) against 419 human anaerobic isolates was determined by the agar dilution method. at concentrations of </=0.5 microgram/ml, telithromycin was active against actinomyces israelii, actinomyces odontolyticus, bacteroides tectum, bacteroides ureolyticus, bacteroides gracilis (now campylobacter gracilis), porphyromonas spp. (including porphyromonas gingivalis and porphyromonas macacae), prevotella intermedia, prevotella heparinolytica, and almost al ...199910543769
activities of gemifloxacin (sb 265805, lb20304) compared to those of other oral antimicrobial agents against unusual anaerobes.the activities of gemifloxacin (sb 265805, lb20304) and comparator agents were determined by an agar dilution method against 419 clinical strains of less-commonly identified species of anaerobes. gemifloxacin was generally more active than trovafloxacin against gram-positive strains by one to two dilutions. peptostreptococci (peptostreptococcus asaccharolyticus, peptostreptococcus magnus, peptostreptococcus micros, and peptostreptococcus prevotii) and porphyromonas spp. (porphyromonas asaccharol ...199910543754
isolation of bacteria from cervical lymph nodes in patients with oral cancer.thirty patients with oral mucosal cancer were studied in relation to oral mucosal damage and bacterial translocation to the regional lymph nodes in the neck. all 30 patients (gingiva 11, tongue 13, cheek mucosa four, oral floor two) underwent extensive, clean-contaminated, head-and-neck surgery (including neck dissection) with free flap reconstruction. a total of 153 lymph nodes was harvested for microbial and histological examination. viable bacteria were isolated from 70 lymph nodes (45.8%) fr ...199910530911
dexamethasone differentially regulates cytokine transcription and translation in macrophages responding to bacteria or okadaic acid.many microorganisms and microbial products induce expression of pro-inflammatory cytokines such as interleukin-1 (il-1alpha/beta) and tumour necrosis factor-alpha (tnf-alpha) in macrophages, primarily by transcriptional activation. we show here, by using mouse macrophages in primary culture, that pre-treatment with dexamethasone inhibits bacteria-induced il-1beta expression as mrna and cellular pro-il-1beta in parallel, consistent with an effect primarily on transcriptional activation. in contra ...199910530874
beta-hemolytic streptococci and other beta-hemolytic organisms in apical periodontitis and severe marginal periodontitis.thirteen teeth with necrotic pulps and apical periodontitis and nine severe periodontal pockets were cultured for presence of beta-hemolytic streptococci and other beta-hemolytic organisms. samples were dispersed and plated on two non-selective and one selective growth media and incubated anaerobically and in 10% co2 in air. a total of 59 beta-hemolytic colonies were purified and identified. eight beta-hemolytic streptococcal isolates were obtained from three of the severe marginal periodontitis ...199910530151
in vitro susceptibility to pexiganan of bacteria isolated from infected diabetic foot ulcers.during two clinical trials involving the treatment of 835 outpatients with infected diabetic foot ulcers, 2515 bacterial isolates, including 2337 aerobes and 178 anaerobes, were grown from cultures of the ulcers. the in vitro susceptibility of these isolates was determined to pexiganan, a peptide anti-infective evaluated in these clinical trials, and to other classes of antibiotics. pexiganan demonstrated broad spectrum antimicrobial activity against gram-positive and gram-negative aerobes and a ...199910529881
microbiological understandings and mysteries of noma (cancrum oris).the microbiologic history of noma was reviewed. studies have associated the disease process with large numbers of fusiform bacilli and spirochetal organisms. in order to study the microbiology of the staging and infection periods of noma 62 nigerian children, aged 3-14 years, 22 children had acute necrotizing ulcerative gingivitis (anug) and were also malnourished, 20 exhibited no acute necrotizing ulcerative gingivitis but were malnourished and 20 were free of acute necrotizing ulcerative gingi ...199910522213
tetracycline-coated polytetrafluoroethylene barrier membranes in the treatment of intraosseous periodontal lesions.periodontal pathogens are detrimental to periodontal healing in barrier membrane-assisted periodontal therapy. tetracycline-coating of barrier membranes may reduce levels of infecting pathogens. this study evaluated the clinical and microbiological effects of tetracycline-coated expanded polytetrafluoroethylene (t-eptfe) barrier membranes in the treatment of 2- to 3-wall intraosseous periodontal lesions around mandibular molars.199910505803
acid production by vaginal flora in vitro is consistent with the rate and extent of vaginal acidification.perinatally, and between menarche and menopause, increased levels of estrogen cause large amounts of glycogen to be deposited in the vaginal epithelium. during these times, the anaerobic metabolism of the glycogen, by the epithelial cells themselves and/or by vaginal flora, causes the vagina to become acidic (ph approximately 4). this study was designed to test whether the characteristics of acid production by vaginal flora in vitro can account for vaginal acidity. eight vaginal lactobacillus is ...199910496892
recurrent acute suppurative thyroiditis in a child: case report.a 23-month-old girl presented with a history of persistent fever and growing left anterior neck mass following an upper respiratory tract infection. laboratory studies revealed leukocytosis, elevated levels of the erythrocyte sedimentation rate and c-reactive protein, and a mild impairment of thyroid function. thyroid scan showed a decreased radioactive iodine uptake of the left thyroid gland. culture of the thyroid aspirate grew the mixed flora, viridans streptococci, prevotella spp, and peptos ...199810496157
microbiology of infected pustular psoriasis lesions.bacterial infections can occur in lesions of pustular psoriasis (pp). the objective of this study was to establish the aerobic and anaerobic microbiology of secondarily infected pp.199910487445
the microbiology of infected and noninfected leg ulcers.a clinical study was undertaken to investigate and compare specifically the aerobic and anaerobic microbiology of infected and noninfected leg ulcers.199910487444
microbiological features of papillon-lefèvre syndrome periodontitis.papillon-lefevre syndrome patients exhibit hyperkeratosis palmo-plantaris and severe periodontitis. the syndrome is an autosomal recessive trait, but the mechanism of periodontal destruction is not known. this report presents the clinical and microbiological features of an 11-year old girl with papillon-lefèvre syndrome. clinical examination included conventional periodontal measurements and radiographic analysis. in samples from 3 deep periodontal lesions, the occurrence of major suspected peri ...199910487314
[clinical evaluation of anaerobic infections in patients with bronchopulmonary infections diagnosed by transtracheal aspiration].we evaluated the clinical and bacteriologic features in the patients with bronchopulmonary infections isolated anaerobes from transtracheal aspirates between april 1990 and march 1998. some anaerobe was isolated in 42 (10.9%) in 387 patients whom we performed transtracheal aspiration (tta), in 42 (15.7%) of 268 in whom some organism was isolated from tta, or in 42 (16.3%) of 257 patients in whom some bacterium excluding acid-fast bacteria, fungi or mycoplasma from tta. the isolation rate of anae ...199910481403
[in vitro bactericidal activities of new oral penem, faropenem against the various clinical isolates].the minimum inhibitory concentration (mic) and minimum bactericidal concentration (mbc) of faropenem (frpm) more compared with those of various oral beta-lactams against 15 isolates each of 6 species of microorganism. frpm possessed potent in vitro antibacterial activity against both aerobic and anaerobic gram-positive bacteria tested. frpm showed the same activity as new oral cephems such as cefdinir, cefditoren and cefcapene against all gram-negative bacteria, but k. pneumoniae strains were le ...199910480050
[bacteria isolated from surgical infections and their susceptibilities to antimicrobial agents: special references to bacteria isolated between july 1996 and june 1997].the annual multicenter studies on isolated bacteria from infections in general surgery and their antimicrobial susceptibility have been conducted in 20 facilities in japan since july 1982. this paper describes the results obtained during period from july 1996 to june 1997. the number of cases investigated as objectives was 217 for one year. a total of 406 strains were isolated from 177 cases (81.6% of total cases). from primary infections 162 strains were isolated, and from postoperative infecti ...199910480049
in vitro activity of gemifloxacin (sb 265805) against anaerobes.gemifloxacin mesylate (sb 265805), a new fluoronaphthyridone, was tested against 359 recent clinical anaerobic isolates by the national committee for clinical laboratory standards reference agar dilution method with supplemented brucella blood agar and an inoculum of 10(5) cfu/spot. comparative antimicrobials tested included trovafloxacin, levofloxacin, grepafloxacin, sparfloxacin, sitafloxacin (du-6859a), penicillin g, amoxicillin clavulanate, imipenem, cefoxitin, clindamycin, and metronidazole ...199910471570
infection-stimulated infraosseus inflammation and bone destruction is increased in p-/e-selectin knockout mice.infections of the dental pulp commonly result in infraosseus inflammation and bone destruction. however, the role of phagocytic leucocytes in the pathogenesis of pulpal infections has been uncertain. in this work we used p/e-/- selectin-deficient mice, which lack rolling adhesion of leucocytes to endothelium and mimic the human syndrome, leucocyte adhesion deficiency ii (lad-ii), to test the hypothesis that phagocytic leucocytes protect against pulpal infection and subsequent periapical infraoss ...199910447722
cervical necrotizing fasciitis: 10 years' experience at a single evaluate clinical and microbiologic characteristics, modalities of treatment and outcome of patients with cervical necrotizing fasciitis admitted to our institution.199910447540
[trovafloxacin in gynecology].presence of microorganisms in the female lower genital tract (lgt) in concentrations that modify the established normal equilibrium produce different symptoms that make necessary to consult the gynecologist. it is currently accepted that infections of the lgt are due to microorganisms that are normally integrating the internal flora, except for the erroneously called "sexually transmitted diseases" that are caused by external microorganisms. this means that all those microorganisms usually prese ...199910436555
activity of telithromycin (hmr 3647) against anaerobic bacteria compared to those of eight other agents by time-kill methodology.time-kill studies examined the activities of telithromycin (hmr 3647), erythromycin a, azithromycin, clarithromycin, roxithromycin, clindamycin, pristinamycin, amoxicillin-clavulanate, and metronidazole against 11 gram-positive and gram-negative anaerobic bacteria. time-kill studies were carried out with the addition of oxyrase in order to prevent the introduction of co(2). macrolide-azalide-ketolide mics were 0.004 to 32.0 microg/ml. of the latter group, telithromycin had the lowest mics, espec ...199910428930
periodontitis associated bacteria in supragingival plaque of dental hygienists: stability of carrier state and clinical development.the purpose of this study was the clinical and microbiological re-examination of dental hygienists, who, 30 months before, had shown remarkably high supragingival levels of periodontitis-associated micro-organisms. interdental plaque was collected from the same molar sites and investigated by the same immunofluorescence assay with taxa-specific monoclonal antibodies as at the initial examination. on average, the 15 re-examined subjects showed slightly increased plaque levels but unchanged bleedi ...199910424388
porphyromonas gingivalis prevalence related to other micro-organisms in adult refractory periodontitis.forty-six adult periodontal patients, selected on the basis of clinical examination, and 46 adult healthy subjects were examined. the subgingival plaque samples from one inflammatory and one non-inflammatory site of each periodontal patient were studied to determine porphyromonas gingivalis prevalence related to other periodontal micro-organisms and to periodontal tissue destruction. the results showed porphyromonas gingivalis as the main pathogenic micro-organism isolated in the inflammatory si ...199910423739
bacteremia after dental treatment in mentally handicapped people.bacteremia may occur after disruption of the oral mucous membrane, particularly after dental treatment. 18 mentally handicapped patients who underwent dental treatment with general anesthesia were included in our study. none of the patients had general illnesses or received antibiotic protection. from each patient several blood samples were drawn aseptically during dental treatment and cultured. the majority of aerobic bacteria recovered belonged to streptococcus sp and gemella sp., anaerobic ba ...199910412852
clinical and microbiological effects of initial periodontal therapy in conjunction with amoxicillin and clavulanic acid in patients with adult periodontitis. a randomised double-blind, placebo-controlled study.the aim of the present study was to investigate the clinical and microbiological effects of initial periodontal therapy in conjunction with systemic amoxicillin plus clavulanic acid in adult periodontitis patients using a double-blind, parallel-group, and placebo-controlled protocol. 21 patients with a clinical diagnosis of generalised adult periodontitis were recruited. clinical measurements and microbiological assessments were carried out at baseline, 3, and 12 months post-treatment. approxima ...199910412851
destructive knee joint infection caused by peptostreptococcus micros: importance of early microbiological diagnosis.peptostreptococcus micros is a commensal of the oral cavity and the genitourinary tract that rarely causes serious infections. a case of a destructive knee joint infection with rapid progress caused by p. micros is presented. the significance of the microbiological findings was initially not acknowledged, which contributed to a nonsuccessful clinical outcome.199910405436
microbiology of retroperitoneal abscesses in children.samples of pus from 41 children with retroperitoneal abscess treated between 1974 and 1994 yielded a total of 125 organisms (3.0 isolates/specimen); 58 isolates were aerobic and facultative species (1.4/specimen) and 67 were anaerobic (1.6/specimen). aerobic bacteria only were isolated from 7 (17%) abscesses, anaerobic bacteria only from 3 (7%) and mixed aerobic and anaerobic bacteria from 31 (76%); 34 (83%) infections were polymicrobial. the predominant aerobic and facultative isolates were esc ...199910403422
inflammatory mediators in saliva of patients with rapidly progressive periodontitis during war stress induced incidence increase.rapidly progressive periodontitis (rpp) results from the interaction between the periodontal microflora and the host. stress is believed to play an important role in determining host responses, and it has been proposed that hyperactivity of host defense mechanisms significantly increases tissue destruction typical for this disease. during a period of four months we have diagnosed 20 patients with acute rpp, all of them active participants in battles of the croatian liberation war with posttrauma ...199910402713
flow cytometry to monitor phagocytosis and oxidative burst of anaerobic periodontopathogenic bacteria by human polymorphonuclear leukocytes.the reduced susceptibility to phagocytosis found among some periodontopathogenic anaerobes may account for the differences between invasive and non-invasive strains. we applied flow cytometry as a powerful tool to analyze and quantify phagocytosis using standardized cultures of oral anaerobes (porphyromonas gingivalis, prevotella intermedia, p. nigrescens, capnocytophaga gingivalis, c. ochracea, c. sputigena, fusobacterium nucleatum and peptostreptococcus micros) and heparinized whole blood. bac ...199910384401
antibiotic susceptibility of putative periodontal pathogens in advanced periodontitis the present study, the antibiotic susceptibility of most prevalent micro-organisms in advanced periodontitis patients was evaluated. in 56 patients, pooled subgingival plaque samples were taken from the deepest site of each quadrant and were cultivated anaerobically. from each patient, the 4 most frequently encountered types of bacterial colonies were subcultured and identified (rapid id 32 a). from all bacterial species identified in the 224 subcultures, the 4 most prevalent were used for su ...199910382573
[multiple abscesses (cerebral, splenic, cutaneous) and pulmonary infection]. 199910365514
effects of hydrogen peroxide on growth and selected properties of porphyromonas this study we first evaluated the effects of hydrogen peroxide (h2o2) on growth and selected properties of porphyromonas gingivalis, and compared them with those obtained by a reducing agent (cysteine). the growth of p. gingivalis was only moderately affected when h2o2 was added at concentrations up to 30 mm in a complex culture medium. however, when a defined basal medium was used, h2o2 at a concentration of 3 mm completely inhibited growth of p. gingivalis. incorporation of cysteine at conc ...199910339828
differential induction of colitis and gastritis in hla-b27 transgenic rats selectively colonized with bacteroides vulgatus or escherichia coli.resident bacteria play an important role in initiating and perpetuating gastrointestinal inflammation. we previously demonstrated that six commensal bacteria including bacteroides vulgatus caused more aggressive colitis and gastritis in hla-b27 transgenic rats than did the other five bacteria without b. vulgatus. this study compared the degree of gastrointestinal inflammation in gnotobiotic hla-b27 transgenic rats monoassociated with either b. vulgatus or escherichia coli. gnotobiotic transgenic ...199910338507
fungi in therapy-resistant apical periodontitis.the occurrence of yeasts in 967 microbiological endodontic samples taken from root canals in persistent endodontic infections was studied. the sampling was done by general practitioners in various parts of finland from root canal infections which did not respond favourably to standard conservative therapy. the samples were cultivated aerobically on a non-selective enriched horse blood agar medium, on tsbv agar medium in 5% co2 and anaerobically on horse blood agar medium. micro-organisms were fo ...199710332243
comparative microbiological characteristics of failing implants and periodontally diseased teeth.the purpose of this report was to compare the distribution of periodontal pathogens recovered from failing implants and teeth with adult and recurrent forms of periodontitis.199910328655
interference by aerobic and anaerobic bacteria in children with recurrent group a beta-hemolytic streptococcal compare the frequency of recovery of aerobic and anaerobic bacteria with interfering capability of group a beta-hemolytic streptococci (gabhs) in the tonsils of children with and without a history of recurrent gabhs pharyngotonsillitis.199910326813
prevalence of corynebacterial 16s rrna sequences in patients with bacterial and "nonbacterial" prostatitis.the etiology of chronic prostatitis syndromes in men is controversial, particularly when positive cultures for established uropathogens are lacking. although identification of bacteria in prostatic fluid has relied on cultivation and microscopy, most microorganisms in the environment, including some human pathogens, are resistant to cultivation. we report here on an rrna-based molecular phylogenetic approach to the identification of bacteria in prostate fluid from prostatitis patients. positive ...199910325338
microbiological and clinical effects of a 1% chlorhexidine-gel in untreated periodontal pockets from adult periodontitis patients.the aim of this study was to report the microbiological and clinical effects of repeated subgingival administration of a 1% chlorhexidine-gel in periodontal pockets from 10 patients with adult periodontitis. results showed that the experimental treatment significantly improved clinical parameters (plaque index, gingival bleeding index, and pocket probing depth). direct subgingival administration of chlorhexidine-gel also produced a remarkable modification in the proportions of putative periodont ...199910322610
the family coriobacteriaceae: reclassification of eubacterium exiguum (poco et al. 1996) and peptostreptococcus heliotrinreducens (lanigan 1976) as slackia exigua gen. nov., comb. nov. and slackia heliotrinireducens gen. nov., comb. nov., and eubacterium lentum (prevot 1938) as eggerthella lenta gen. nov., comb. nov.16s rrna gene sequences were determined for eubacterium exiguum and peptostreptococcus heliotrinreducens. these species were found to be closely related and, together with eubacterium lentum, to constitute a branch of the coriobacteriaceae. two new genera are proposed on the basis of phenotypic characteristics and 16s rrna gene sequence comparisons: slackia to include the bile-sensitive species eubacterium exiguum and p. heliotrinreducens, and eggerthella to include the bile-resistant eubacteriu ...199910319481
bacterial populations contaminating the upper gut in patients with small intestinal bacterial overgrowth syndrome.small intestinal bacterial overgrowth syndrome (sibos) is characterized by an abnormally high bacterial population level in the upper gut, exceeding 10(5) organisms/ml (5 log colony-forming unit (cfu)/ml). to understand its origin and select an appropriate antibiotic treatment, we have analyzed the bacterial populations contaminating the upper gut in sibos patients.199910235214
interactions between a single immunoglobulin-binding domain of protein l from peptostreptococcus magnus and a human kappa light chain.the placement of a tryptophan residue into a single ig-binding-domain of protein l from peptostreptococcus magnus has been used to examine the binding interactions between the binding domain and kappa light chains (kappa-chains). the fluorescence intensity of the mutant domain increases on the formation of a complex with kappa-chains. this has been used to determine the kd of the complex under a range of conditions by using both pre-equilibrium and equilibrium methods. the kd values determined f ...199910229674
in vitro activities of pazufloxacin, a novel injectable quinolone, against bacteria causing infections in obstetric and gynecological patients.t-3762 is an injectable new quinolone with a broad spectrum of antibacterial activity. pazufloxacin (t-3761) is an active form of t-3762. the minimal inhibitory concentrations (mics) of pazufloxacin for 50% of the clinical isolates tested were 3.13 microg/ml for streptococcus agalactiae, 6.25 microg/ml for gardnerella vaginalis, 0.025 microg/ml for escherichia coli, 0.78 microg/ml for pseudomonas aeruginosa, 6.25 microg/ml for peptostreptococcus magnus, 6.25 microg/ml for bacteroides fragilis an ...199910224336
metronidazole susceptibility testing of anaerobic bacteria associated with periodontal disease.the aim of the present investigation was to determine the susceptibility of prevotella intermedia, porphyromonas gingivalis, fusobacterium and peptostreptococcus micros to metronidazole in vitro. two methods were applied on each isolated strain: agar dilution and epsilometer etest. a total of fifty three wild test strains (13 p.intermedia, 14 p. gingivalis, 14 f.spp and 12 p.micros) were isolated from patients with periodontitis. the etest appears to be a simple, rapid and reliable method for th ...199910223399
coagulase-negative staphylococci are the most common bacteria found in cultures from the deep portions of hidradenitis suppurativa lesions, as obtained by carbon dioxide laser surgery.the significance of bacterial findings in hidradenitis suppurativa (hs) is controversial. interpretation of the results of bacteriological examinations from the surface of hs lesions is obscured by the possible contamination of resident skin bacteria. bacteriological analysis of aspirates from deeper parts of hs is liable to show low sensitivity. we used a carbon dioxide (co2) laser method to evaporate the diseased tissue level by level from the surface downwards, allowing concurrent sampling of ...199910215774
adherence of peptostreptococcus micros morphotypes to epithelial cells in vitro.peptostreptococcus micros, which is associated with oral and non-oral mixed anaerobic infections, occurs in three colony morphotypes, the smooth type, the rough type and the smooth variant of the rough type. these types differ in surface structures; the rough type expresses large fibrillar surface appendages, which are absent on the surface of both the smooth and the smooth variant of the rough type. to determine the role of these surface structures in adherence we characterized the adherence of ...199910204480
coaggregation of candida dubliniensis with fusobacterium nucleatum.the binding of microorganisms to each other and oral surfaces contributes to the progression of microbial infections in the oral cavity. candida dubliniensis, a newly characterized species, has been identified in human immunodeficiency virus-seropositive patients and other immunocompromised individuals. c. dubliniensis phenotypically resembles candida albicans in many respects yet can be identified and differentiated as a unique candida species by phenotypic and genetic profiles. the purpose of ...199910203506
cloning of fiba, encoding an immunogenic subunit of the fibril-like surface structure of peptostreptococcus micros.although we are currently unaware of its biological function, the fibril-like surface structure is a prominent characteristic of the rough (rg) genotype of the gram-positive periodontal pathogen peptostreptococcus micros. the smooth (sm) type of this species as well as the smooth variant of the rg type (rgsm) lack these structures on their surface. a fibril-specific serum, as determined by immunogold electron microscopy, was obtained through adsorption of a rabbit anti-rg type serum with excess ...199910198013
intra-operative ultrasound-guided drainage of parotid abscess.parotitis complicated by parotid abscess remains a potentially life-threatening problem. conventional surgical treatment involves incising the parotid parenchyma in the direction of the facial nerve until the abscess is located and evacuated. intra-operative ultrasound greatly assists in localizing the abscess and in ensuring its complete drainage. expeditious and exact localization of the abscess reduces operative time. equally importantly, ultrasound-assisted drainage reduces surgical dissecti ...199810197156
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