phase variation of hyaluronidase-producing peptostreptococci associated with periodontal disease.peptostreptococci isolated from diseased periodontal pockets produced large amounts of extracellular hyaluronidase throughout their growth cycle. phase variants, obtained by re-streaking aged colonies of the wild types, had different colonial morphology and shorter generation times. these variants produced less hyaluronidase, and enzyme production occurred only during the early stationary phase.19836575993
[recently detected organisms from oral infectious diseases and their antimicrobial sensitivity]. 19846597502
experimental otitis media in guinea pigs. serum antibody response to bacteroides fragilis, propionibacterium acnes and peptostreptococcus anaerobius upon challenge.the production of antibodies in serum was studied after inoculation of bacteroides fragilis, propionibacterium acnes or peptostreptococcus anaerobius into the middle ear of guinea pigs. inoculation of b. fragilis resulted in igg and igm serum antibodies at an early stage of the infection, while iga antibodies were seldom detected and at a later stage. in contrast, inoculation of p. acnes or p. anaerobius induced no serum antibody response. of these three species b. fragilis induced the most inte ...19846598260
gardnerella vaginalis and anaerobic bacteria in genital a study of gardnerella vaginalis and anaerobic bacteria in non-specific vaginitis (nsv) and other genital disease 89 patients attending a genital medicine clinic had vaginal samples examined for conventional pathogens and for quantitative analysis of g vaginalis and aerobic and anaerobic bacterial flora. the overall incidence of g vaginalis was 20%; g vaginalis (mean concentration 7.0 log10/g of secretion) occurred predominantly in patients with nsv (57%) but also in sexual contacts of non-sp ...19836600955
b-lymphocyte blastogenesis in response to periodontitis-associated bacteria. kinetics and proportion of total response.a role for activated b-lymphocytes in mediating the initiation and/or progression of periodontal diseases has been proposed in previous work. the present study was conducted to: (1) assess the proportion of total lymphocyte blastogenic response to selected oral bacteria which is composed of t-cell and b-cell activation, respectively, and (2) to determine whether different kinetic patterns exist for the t-cell vs. the b-cell responses to these bacteria. using lymphocyte rosetting and culturing te ...19846610740
an international study of agglutinins to eubacterium, peptostreptococcus and coprococcus species in crohn's disease, ulcerative colitis and control subjects.the world-wide occurrence of agglutinating antibodies to four coccoid anaerobes belonging to eubacterium, peptostreptococcus and coprococcus spp. was investigated in 937 coded sera from patients suffering from crohn's disease, ulcerative colitis, various other diseases and from healthy controls. positive results were found in 59% of patients with crohn's disease, 29% of patients with ulcerative colitis, and 8% of both diseased and healthy control subjects. patients with crohn's disease of the co ...19836628839
bacteriology of dental abscesses of endodontic origin.aspirates have been cultured from 10 dental abscesses of endodontic origin, all of which had penetrated beyond the bony alveolus to produce fluctuant swelling. sampling was by syringe aspiration. strict anaerobic techniques, including the use of an anaerobic chamber, were used for serial dilution and plating. randomly selected colonies (100) from each culture were purified, characterized, and identified. seventy percent of the bacterial isolates were either strict anaerobes or microaerophilic. o ...19836630460
tubo-ovarian abscess: contemporary approach to management.two hundred and thirty-two patients with tubo-ovarian abscesses (toas) were evaluated. ruptured toas were documented in seven (3%) of the patients. one hundred and seventy-five patients with toas were treated with antibiotics alone; for 15 of these patients, toas were confirmed by laparoscopy. the remaining 57 patients required surgical intervention: drainage (five patients), unilateral salpingo-oophorectomy (19) and total abdominal hysterectomy and bilateral salpingo-oophorectomy (33). a unilat ...19836635426
death of an african elephant from probable toxemia attributed to chronic pulpitis.a 31-year-old captive male african elephant (loxodonta africana) of 5,000-kg body weight died suddenly in ventral recumbency. lesions seen at necropsy were bilateral purulent pulpitis and periodontitis of both tusks, serous atrophy of coronary groove fat, grammocephalus cholangitis, myocardial and skeletal lipofuscinosis, and scattered segmental necrosis in the pectoral muscles. nonhemolytic streptococci, corynebacterium sp, peptostreptococcus anaerobius, fusobacterium nucleatum, and bacteroides ...19836643242
[experimental and clinical evaluation of cefroxadine in the field of obstetrics and gynecology].cefroxadine (cxd) was studied in the field of obstetrics and gynecology and the following results were obtained. mic values of cxd against 55 clinical isolates were investigated, from 6.25 to 12.5 micrograms/ml for 8 strains of e. coli, 6.25 micrograms/ml for 3 strains of k. pneumoniae and from 0.39 to 3.13 micrograms/ml for 9 strains of anaerobes such as peptostreptococcus and peptococcus in 10(6) cells/ml. transfer of cxd into intrapelvic organ such as uterus, uterine tube and ovary after the ...19836655868
[clinical study of cefroxadine in the field of obstetrics and gynecology].clinical study of cefroxadine (cxd), an orally active cephalosporin, for the treatment of infections in the field of obstetrics and gynecology was carried out and the following results were obtained. fifteen strains were isolated in present study. these isolates were mainly e. coli and anaerobes (peptococcus sp., peptostreptococcus sp.). the distribution of susceptibilities to cxd of e. coli was between 6.25 approximately 12.5 micrograms/ml and that of anaerobes was between 0.39 approximately 1. ...19836655870
the effect of lincomycin-neomycin treatment on experimental anaerobic bacterial bovine mastitis.three healthy lactating quarters of a friesland cow were each experimentally infected with a pure culture of a strain of either bacteroides fragilis, eubacterium lentum or a peptostreptococcus sp. respectively. the onset and progression to clinical mastitis was monitored 12 hourly by examination for clinical signs of inflammation, bacterial culture, somatic cell counts and with a strip cup. all infected quarters developed clinical mastitis within 24 hours. the 2 quarters infected with b. fragili ...19836668573
selected bacterial antibodies in crohn's disease and ulcerative colitis.agglutinins to four strains of anaerobic gram-positive coccoid rods (species of eubacterium, peptostreptococcus and coprococcus) were found in significantly higher frequency in crohn's disease (cd) than in ulcerative colitis (uc) and in other diseased control subjects and were virtually absent in apparently healthy subjects. when the posterior probability of having cd was calculated on the basis of these agglutination reactions, 64% of patients with cd and 34% of patients with uc but only 10% of ...19836673050
[clinical evaluation of tinidazole on anaerobic infections in the field of obstetrics and gynecology].the bacteriological and clinical effect of tinidazole (tdz) was evaluated in 16 cases of intrauterine, intrapelvic and vulvar infection caused by anaerobic organisms and the following results were obtained. anaerobes were detected in 16 cases, including 1 case with anaerobes alone and 15 cases with mixed anaerobes and aerobes. eight different species and 24 strains were detected. a single species was isolated from 9 cases, 2 species from 6 cases and 3 species from 1 case. the main species detect ...19836674545
in vitro activity of u-57930e against anaerobic bacteria and its comparison with clindamycin, ampicillin, carbenicillin and tetracycline.the in vitro activity of u-57930e, a pipecolic acid amide of clindamycin, was compared with those of clindamycin, ampicillin, carbenicillin and tetracycline against 321 anaerobic clinical isolates. the mic (micrograms/ml) of u-57930e that inhibited 95% bacteroides fragilis, peptococcus prevotii, b. melaninogenicus and p. asaccharolyticus was 0.0625; 0.03125 for peptostreptococcus anaerobius, b. vulgatus, propionibacterium and peptococcus species. clindamycin, on the other hand, gave mic values o ...19836678915
agglutinins to anaerobic bacteria in crohn's disease and in indian patients with diarrhoea.agglutinins to certain species of eubacterium and peptostreptococcus have been reported in sera from a high proportion of patients with crohn's disease. because this might be a non-specific finding common to patients with diarrhoea associated with damaged intestinal mucosa, we have compared the incidence of such agglutinins in patients with crohn's disease with that seen in patients in north-east india with acute or chronic diarrhoea. the incidence of agglutinins in crohn's disease was 44%, comp ...19846708086
bactericidal and cytotoxic effects of hypothiocyanite-hydrogen peroxide mixtures.lactoperoxidase catalyzes the oxidation of thiocyanate by hydrogen peroxide into hypothiocyanite, a reaction which can protect bacterial and mammalian cells from killing by hydrogen peroxide. the present study demonstrates, however, that lactoperoxidase in the presence of thiocyanate can actually potentiate the bactericidal and cytotoxic effects of hydrogen peroxide under specific conditions, such as when hydrogen peroxide is present in the reaction mixtures in excess of thiocyanate. the toxic a ...19846724690
osteomyelitis in dogs and cats caused by anaerobic bacteria.localised osteomyelitis was diagnosed in 2 dogs and 2 cats. the disease was caused by fight wounds in 3 cases. radiographic examination demonstrated a circumscribed zone of cortical bone lysis, sequestra and periosteal new bone. each case was treated surgically by sequestrectomy and debridement. infection was due mainly to anaerobic bacteria. the pathogenic bacteria isolated from the lesions of dogs were actinomyces viscosus, fusobacterium nucleatum and bacteroides spp, and from the lesions in c ...19846732670
radiological case of the month. anaerobic pyopneumopericardium. 19846741898
pathogenicity of anaerobic gram-positive cocci.the pathogenicity of 20 strains of facultative or anaerobic gram-positive cocci (agpc) was investigated by injecting them alone or mixed with other flora into mice, utilizing the subcutaneous abscess model. abscesses induced by a mixture of two organisms were uniformly larger than those induced by single organisms. the relationships among seven agpc strains, eight aerobes, and two bacteroides spp. were determined by treating the infected animals with antibiotics and observing the effect of thera ...19846746091
a note on fermentation reactions of anaerobic bacteria on a solid medium. 19826749787
antibody reactive with peptostreptococcus micros in the sera of patients with periodontal disease.subgingival plaque samples were cultured for the isolation of peptostreptococcus micros from individuals with and without chronic periodontitis. humoral antibody from each person reacted with p. micros in an indirect fluorescent-antibody test, but not all of the sera reacted in a passive hemolysis test. no correlations were observed between the presence of antibody reactive with p. micros and the isolation of p. micros or the gingival health of the individual.19826749898
vaginal organisms in prepubertal children with and without vulvovaginitis. a vaginoscopic a prospective clinical and microbiological study covering 67 prepubertal children the vaginal flora of 31 asymptomatic children was compared to 36 children suffering from abnormal vaginal discharge and/or vulvovaginitis. vaginoscopy was employed to obtain bacteriological specimens without contamination. swabs were inoculated into prereduced transportmedia (port a cul, bbl) and cultured aerobically and anaerobically. the most prevalent aerobic organisms were staphylococcus epidermidis. enteroc ...19826751240
the use of an anaerobic incubator for the isolation of anaerobes from clinical anaerobic incubator was compared with a standard jar system for the isolation of anaerobes from clinical material. seventy specimens were selected as likely to yield anaerobes: 342 different anaerobes were isolated in the incubator and 347 in anaebrobic jars. these included bacteroides spp (43%), peptococcus spp (26%), peptostreptococcus spp (13%), veillonella spp (7%), fusobacterium spp (7%), clostridium spp (2%) and miscellaneous gram-positive nonsporing bacilli (2%). differences in isolati ...19826752210
[evaluation of clinical efficacy of a cephem antibiotic, cefmetazole, in inflammatory infections of the upper respiratory tract].we had reported the fundamental study on the utility of a new cephem antibiotic, cefmetazole (cmz). on the basis of the results we administered cmz to patients to investigate its clinical utility in this study. 1. cmz was administered to 53 patients including 4 infants. they consisted of 30 cases of acute tonsillitis, 10 of peritonsillar abscess, 10 of laryngitis or pharyngitis, and 3 of sinusitis. 2. one of 2 g cmz was administered to an adult patient except for l case and 0.5 g to an infant pa ...19826752466
isolation of anaerobic organisms from kidney in serious renal infections.we have studied 45 patients who underwent nephrectomy owing to unilateral renal sepsis for anaerobic and aerobic bacterial growth in the urine and kidney. anaerobic organisms were recovered from 11 patients: 10 had positive kidney cultures, and only 1 had positive kidney and urine cultures. there was a distinct relationship between anaerobic infection of the kidney and urinary tract obstruction; 44 per cent of the obstructed kidneys yielded anaerobic organisms versus only 11 per cent of the unob ...19826755857
bacteriologic and serologic investigation in diffuse sclerosing osteomyelitis (dso) of the attempt was made to clarify the obscure origin of diffuse sclerosing osteomyelitis (dso) of the mandible. bacteriologic investigations of specimens from diseased mandibles were performed with special attention to anaerobic culture technique. propionibacterium acnes and peptostreptococcus intermedius were in some patients found to be of etiologic importance. sampling techniques and associated problems are discussed. an examination was made for antibodies in the patients' sera against antigens ...19826757825
achromopeptidase for lysis of anaerobic gram-positive cocci.achromopeptidase, which has potent bacteriolytic activity for most of the gram-positive aerobic bacteria, was for the first time used for the lysis of anaerobic cocci. most of the lysozyme-resistant gram-positive anaerobic cocci were lysed with this new enzyme. peptococcus magnus was the only organism tested resistant to achromopeptidase. p. saccharolyticus was quite unusual because it was very sensitive to both achromopeptidase and lysozyme.19826759529
antibiotic resistance in anaerobic bacteria: molecular biology and clinical aspects.the patterns of antibiotic susceptibility among anaerobes isolated in the united states during the past 14 years were reviewed. resistance to the tetracyclines, the penicillins, clindamycin, and other antibiotics has emerged among strains of bacteroides, clostridium, and anaerobic cocci. genetic transfer of antibiotic resistance has been documented in anaerobic environments. inter- and intrageneric transfer by conjugation and transformation has been described. plasmids have been identified in an ...19826760336
antigenic relationships and rapid identification of peptostreptococcus species.antisera against whole cells of each peptostreptococcus species (p. anaerobius, p. micros, p. parvulus, and p. productus) were produced in rabbits. when these antisera were reacted against sonically disrupted cells and culture supernatant fluids in ouchterlony tests, lines of identity were obtained among the antigens from all the species and uninoculated culture medium. when the antisera were subsequently absorbed with the dehydrated culture medium used to grow the peptostreptococci, all cross-r ...19806769956
kinetic parameters of lactate dehydrogenases of some rumen bacterial species, the anaerobic ciliate isotricha prostoma and mixed rumen microorganisms.a number of kinetic parameters of the lactate dehydrogenases of three rumen bacterial species (peptostreptococcus productus, propionibacterium acnes and actinomyces viscosus), the rumen ciliate isotricha prostoma and mixed rumen microorganisms (mrm) with respect to nadh, pyruvate, fructose-1,6-diphosphate (fdp) as well as the effects of several nucleotide phosphates were studied. partially purified ldh of peptostr. productus had the same kinetic parameters as in crude cell free extracts. values ...19806778389
serum antibodies to anaerobic coccoid rods in patients with crohn's disease or ulcerative colitis, and in medical and nursing staff. 19816779893
bacterial interference between clostridium difficile and normal fecal flora.clostridium difficile has been shown to be the cause of virtually all cases of pseudomembranous colitis related to the administration of antimicrobial agents. it is possible that some antimicrobial agents alter the normal bacterial flora of the gastrointestinal tract so as to permit colonization and/or proliferation by c. difficile. the inhibitory activity of representative fecal bacteria from 23 anaerobic and aerobic genera against c. difficile was examined using two in vitro procedures. strain ...19816785366
the fermentation of lactulose by colonic bacteria.sixty-four strains of intestinal bacteria were cultured under anaerobic conditions in lactulose-containing media to assess their ability to ferment lactulose. some organisms were unable to metabolize the disaccharide, while others, e.g. clostridia and lactobacilli, metabolized lactulose extensively. quantitative analyses of the fermentation products indicated that the major non-gaseous metabolites were acetic, lactic and butyric acids. hydrogen and carbon dioxide were the only gases detected. fe ...19826804597
leucine dissimilation to isovaleric and isocaproic acids by cell suspensions of amino acid fermenting anaerobes: the stickland reaction revisited.freshly compared cell suspensions of clostridia (clostridium bifermentans, c. botulinum proteolytic type a, c. difficile, c. sordellii, and c. sporogenes) and peptostreptococcus anaerobius converted leucine to isovaleric (iv) and isocaproic (ic) acids in the absence of other amino acids. the optimal ph for conversion was between 8 and 9 at 37 degrees c. the stoichiometry of reaction was compatible with that expected for the stickland reaction, as the ratio of iv to ic was 1:2, the amount of co2 ...19826805929
pediatric infectious cervical lymphadenitis.infectious cervical lymphadenitis is a frequently encountered problem in the pediatric population. the recent experience with infectious cervical lymphadenitis at the children's hospital of philadelphia was reviewed. the most common etiologic agent was staphylococcus aureus. beta-hemolytic streptococcus and mycobacterium were found in 15% and 6% of cases respectively. other agents included pneumococcus, cat scratch, toxoplasma gondii, actinomycosis, and mixed bacteroides and peptostreptococcus. ...19806821410
effect of glucose concentration in the growth medium upon neutral and acidic fermentation end-products of clostridium bifermentans, clostridium sporogenes and peptostreptococcus anaerobius.clostridium bifermentans, c. sporogenes and peptostreptococcus anaerobius were grown in fastidious anaerobe broth with glucose concentrations from 0-1.5% (w/v). gas chromatographic (glc) analysis of fermentation end-products revealed that a change in glucose concentration markedly affected fermentation patterns of each microorganism. increased glucose concentration resulted in stimulation of ethanol production by all strains, and of butanol and butyric acid by c. sporogenes. with c. bifermentans ...19836822993
anaerobic pyogenic sacroiliitis. 19836844970
perineal crohn's disease complicated by pyogenic liver abscess during metronidazole therapy.a patient with ileal and perineal crohn's disease developed a pyogenic liver abscess caused by streptococcus milleri. the perineal process had been treated with high-dose metronidazole for the preceding 6 mo, and culture of a perianal abscess 2 mo before admission revealed heavy growth of streptococcus milleri (which was misidentified as an anaerobic peptostreptococcus). this case illustrates the difficulty in correctly identifying streptococcus milleri, the most frequent isolate from pyogenic l ...19836852451
anaerobic bacteria associated with osteomyelitis in domestic animals.specimens of bony tissue or adjacent soft tissue from 19 animals with osteomyelitis were cultured aerobically and anaerobically. fourteen specimens (74%) yielded anaerobic bacteria in pure culture or mixed with aerobic or facultative anaerobic bacteria. the most predominant genus encountered was an obligate anaerobe, bacteroides. the most frequently isolated bacteroides species was bacteroides asaccharolyticus, which was isolated 5 times. the most frequently isolated anaerobe was peptostreptococ ...19836853311
effect of eight growth media upon fermentation profiles of ten anaerobic bacteria.a study was made of the influence of eight growth media upon the fermentation end-product patterns obtained with ten species of anaerobes. acidic and neutral end-products of fermentation were analysed by gas chromatography and the results were examined statistically by computer. differences in end-product profile were at least as great between different media used to grow a single anaerobic species, as between different anaerobes grown in a single type of medium. when individual fermentation end ...19836853401
aspiration and lung abscess in cystic fibrosis.large abscess cavities are not commonly encountered in patients with cystic fibrosis (cf). one such patient is reported in whom an abscess developed secondary to aspiration of sand and salt water. material obtained at bronchoscopy revealed anaerobes as well as the aerobic organisms previously cultured from her sputum. rapid improvement occurred after institution of antibiotic therapy specifically directed against the anaerobic organisms.19836859663
[nonclostridial anaerobic soft tissue infections].fourteen cases of nonclostridial anaerobic infections of soft tissues caused by peptococci, peptostreptococci, bacteroids or their combinations with e. coli, proteus, and staphylococcus aureus were studied. they are characterized by a rapid spread of serous-suppurative inflammation and the causative agents in loose fibrous connective tissue of subcutaneous adipose tissue (cellutitis), surface fascia (fasciitis), or skeletal muscles (myositis). dissolution of intercellular structures of the conne ...19836860161
ct in the diagnosis and follow-up of multiple abscesses in the liver.computed tomography (ct) in the diagnosis of liver abscesses is discussed. the accuracy of ct scan in differentiating hepatic masses is reviewed. two ct control scans were performed during the follow-up period.19836872821
[serodiagnosis of crohn's disease].three serological tests, recommended as being of diagnostic value for crohn's disease, were evaluated in 39 patients with crohn's disease and--as controls--in 27 patients with ulcerative colitis, 45 healthy persons and 65 patients with inflammatory diseases other than crohn's disease or ulcerative colitis. the tests were the determination of (1) serum antibodies to pseudomonas-like organisms (plo) by means of indirect immunofluorescence; (2) agglutinating serum antibodies to 4 strains of anaerob ...19836872878
anaerobic bacteria in uterine infection following first trimester abortion.samples of uterine fluid and cervical mucus for bacterial culture were obtained from 23 patients with endometritis following first trimester abortion. uterine samples were obtained via a sheathed system which minimizes contamination by the cervical flora. a mean of 3.4 isolates were identified in cervical samples as compared to 1.8 in uterine samples. anaerobic bacteria were found in 57% of the uterine samples and dominated in both uterine and cervical samples. peptostreptococcus species dominat ...19826890898
heparin degradation by eubacterium and peptostreptococcus species from bovine endometritis. 19826890997
[a bacteriological study of peritonsillar abscess and its chemotherapy (author's transl)]. 19806942141
n-formimidoyl-thienamycin activity against anaerobes: effect of the inoculum, ph and culture media. 19816945303
bacteriologic evaluation of the efficacy of mechanical root canal instrumentation in endodontic therapy.the presence of bacteria in 17 single-rooted teeth, with periapical lesions, was studied throughout a whole period of treatment. the root canals were irrigated with physiologic saline solution during instrumentation. no antibacterial solutions or dressings were used. bacteria were found in all initial specimens from the teeth (median number of bacterial cells 4 x 10(5), range 10(2) - 10(7)) and the number of strains in the specimens ranged from 1 to 10.88% of the strains were anaerobic. the most ...19816947391
[comparative study of the flora of the pocket and gingival sulcus]. 19816949889
[study on anaerobic gram-positive cocci in human dental plaque (author's transl)]. 19816950002
n-formimidoyl thienamycin (mk0787): in vitro activity against anaerobic bacteria.the in vitro activity of n-formimidoyl thienamycin (mk0787) was tested against 239 anaerobic bacteria clinical isolates: 70 of bacteroides fragilis, 18 of b. distasonis, 16 of b. thetaiotaomicron, 10 of b. vulgatus, 24 of bacteroides spp., 22 of b. melaninogenicus (all three subspecies), 26 of fusobacterium spp., 10 of peptococcus spp., 15 of peptostreptococcus spp., 15 of clostridium perfringens, and 13 of clostridium spp. ninety-five percent of the isolates were inhibited by less than or equal ...19826952818
characterization of nadp-dependent 7 beta-hydroxysteroid dehydrogenases from peptostreptococcus productus and eubacterium aerofaciens.peptostreptococcus productus strain b-52 (a human fecal isolate) and eubacterium aerofaciens atcc 25986 were found to contain nadp-dependent 7 beta-hydroxysteriod dehydrogenase activity. the enzyme was synthesized constitutively by both organisms, and the enzyme yields were suppressed by the addition of 0.5 mm 7 beta-hydroxy bile acid to the growth medium. purification of the enzyme by chromatography resulted in preparations with 3.5 (p. productus b-52, on sephadex g-200) and 1.8 (e. aerofaciens ...19826954878
circulating antibodies after experimental chronic infection in the root canal of teeth in monkeys.root canals of 49 teeth with necrotic pulp tissue in five monkeys were infected with streptococcus faecalis ss liquefaciens, actinomyces bovis, fusobacterium nucleatum, peptostreptococcus anaerobius, and bacteroides oralis in various combinations. after 6 months the root canals and the periapical tissues were subjected to radiographic, bacteriologic, and histologic examinations. signs of periapical inflammation were radiographically registered in 41 teeth, most frequently in teeth inoculated wit ...19826960463
[evaluation of humoral response to selected bacterial antigens in dental focal infections]. 19826964085
[assessment of the leukocyte migration inhibition test in response to selected bacterial antigens from dental focal infections]. 19826964137
bacteriologic study of paronychia in children. 19816972709
[experimental study of the pathogenicity of anaerobic streptococci (peptostreptococci)]. 19816977248
[clinico-microbiologic bases for the use of new cephalosporins]. 19826979006
[studies on in vitro susceptibility of various anaerobic bacteria and antimicrobial resistance of anaerobes (author's transl)]. 19826979640
identification of attached bacteria in the nasopharynx of the child.bacteria attached to epithelial cells in mucus and cell scrapings obtained from the nasopharynx in children undergoing adenoidectomy appear rather monomorphic under the microscope, and for this reason it is not possible to identify different species. to overcome this difficulty, a micromanipulation method has been devised which allows the isolation of single squamous epithelial cells seen to carry bacteria. the cells are transferred to culture media for bacteriological analysis. ciliated epithel ...19826980192
microorganisms isolated from peritonsillar abscess and indicated chemotherapy.a bacteriologic study was made of 30 patients with peritonsillar abscesses with the use of both aerobic and anaerobic culture procedures. the abscess was punctured and the pus was aspirated by a syringe with an 18-gauge needle. aerobes and anaerobes were detected in a frequency rate of approximately 1:2. peptostreptococcus (30.2%), group a streptococci (27.9%), peptococcus (16.3%), and fusobacterium (9.3%) were preponderant pathogens. anaerobic organisms were isolated in 75% of 30 cases. aside f ...19826982036
microorganisms associated with abscesses of sheep and goats in the south of iran.a total of 86 abscesses (45 and 41) abscesses of sheep and goats, respectively) were examined for their causal agents; 44 of these abscesses were located subcutaneously, and the remaining 42 were in lungs, livers, intestines, and udders. a total of 23 different types of microorganisms were isolated from 78 abscesses; bacteria were not detected in the remaining eight abscesses. microorganisms isolated were: species of the genera corynebacterium, staphylococcus, and streptococcus and pasteurella, ...19806996545
introduction of anaerobic methodology into a clinical microbiological laboratory.for the successful introduction of anaerobic methodology into a clinical microbiological laboratory, several factors are very important. these include: 1) laboratory; 2) establishment of skilful technicians and adequate equipment; and 3) communication with clinicians who believe in anaerobes as infectious agents and are interested in working with the microbiologist. the beneficial effects of these factors on a laboratory's efficiency in providing an anaerobic service are demonstrated by the foll ...19807005099
microbial flora of the lower genital tract of women in labour in zaria, nigeria.nine genera of microbes isolated from the lower genital tract of 187 women in labour in zaria have been identified. the work was undertaken to establish the nature of microorganisms in the lower genital tract of women in labour as a basis for further study. the isolates in order of prevalence were: candida albicans (20.9%), klebsiella sp (15.0%), escherichia coli (9.1%), streptococcus faecalis (6.4%), haemolytic streptococci (other than streptococcus pyogenes (2.7%), streptococcus viridans (2.1% ...19817007447
puerperal sepsis in port moresby, papua new guinea.because puerperal sepsis is the second most common cause of maternal death in papua new guinea some aspects of its aetiology and prevention have been investigated. 91% of 125 women with puerperal fever were found to have post-partum uterine infection. the anaerobes bacteroides and peptostreptococcus singly or together, were the commonest causes, while streptococcus pyogenes was the predominant aerobic species. antibiotic sensitivity patterns were established, and chloramphenicol was found to be ...19807008426
premature labor. ii. bacterial sources of phospholipase.human term labor is thought to be initiated by amniotic and chorionic phospholipase a2, an enzyme that liberates arachidonic acid esters from the phospholipids of these membranes, leading to the synthesis of prostaglandins by the placental membranes. the striking association of premature labor with intrauterine infection or contamination, urinary tract infection, and early neonatal sepsis led us to study the microorganisms present in these infections for phospholipase a2 activity. activity was f ...19817017516
anaerobic bacteria in pediatric respiratory infection: progress in diagnosis and treatment. 19817017948
[saprophytic and opportunistic non spore-forming anaerobic microflora of the vagina (author's transl)].a microbiological survey has been carried out on 179 healthy, child-bearing aged, non-pregnant women, with the aim to evaluate the incidence of anaerobic non-spore forming bacteria in the normal vaginal flora. this group of bacteria has been isolated in 50.3% of women, with a clear prevalence of "anaerobic streptococci " and bacteroides, followed by fusobacterium and veillonella. no propionibacterium, eubacterium or bifidobacterium have been isolated. according to many authors the non-spore form ...19807018415
the faecal flora of patients with crohn's disease.the faecal flora of patients with crohn's disease was compared with that of healthy subjects. in patients with terminal ileitis, numbers of anaerobic gram-negative and coccoid rods (species of eubacterium and peptostreptococcus) were higher than in the controls whereas anaerobic gram-positive rods and cocci and aerobes occurred in normal numbers. the composition of the flora was neither influenced by duration of the disease nor by ileocaecal resection. in healthy subjects and patients, a chemica ...19817019315
serum agglutinins to eubacterium and peptostreptococcus species in crohn's and other diseases.sera from patients suffering from crohn's and other diseases and from healthy subjects were tested for agglutinins to anaerobic, gram-positive coccoid rods belonging to species of eubacterium and peptostreptococcus. four strains labelled eubacterium contortum (two strains), eubacterium rectale and peptostreptococcus productus were agglutinated by a higher percentage of sera from patients with crohn's disease than from healthy subjects and from patients with liver and intestinal diseases (includi ...19817019318
colonic bacteria: a source of gamma-aminobutyric acid in blood. 19817019921
[endometrial bacteriology in puerperal infections (author's transl)].from the third to the sixth day postpartum transcervical endometrial swabs were taken from 51 women with the clinical signs of endometritis, like fever over 38 degree c, uterine tenderness and purulent lochia using prereduced anaerobic transport-media. the specimens were cultured aerobically and anaerobically. 27 women had an endometritis following vaginal delivery, 24 following caesarian section. following vaginal delivery aerobic bacteria were isolated in 85.2% of the cases and anaerobic bacte ...19817032100
evidence that the greening ligand in native butyryl-coa dehydrogenase is a coa persulfide.yellow butyryl-coa dehydrogenase and general acyl-coa dehydrogenase are "greened" by a mixture of coenzyme a plus elemental sulfur. the resultant stable complex contains an identical ligand with that present in native green butyryl-coa dehydrogenase and has the same broad absorption band centered at 710 nm. evidence is presented that the greening ligand is a coa persulfide, possibly a mimic of the substrate carbanion thought to be generated early in the normal catalytic cycle. variation in the p ...19827068637
intracranial complications of sinusitis in children. a sequela of periapical abscess.the cases of two children with periapical abscess in the upper incisors, sinusitis, and intracranial abscess are described. the ethmoid and maxillary sinuses were involved in both patients. subdural empyema occurred in both, and one of the children had also cerebritis and brain abscess. anaerobic bacteria were isolated from the infected subdural empyemas. peptostreptococcus intermedius and microaerophilic streptococci were recovered in one patient and fusobacterium sp in the other. surgical drai ...19827073175
streptococcus pneumoniae and three species of anaerobic bacteria in experimental otitis media in guniea pigs.three species of anaerobic bacteria commonly isolated in upper respiratory tract infections in humans were studied in an animal model of otitis media. the ability of propionibacterium acnes to induce acute otitis media was comparable with that of streptococcus pneumoniae, the classic middle ear pathogen. the response to peptostreptococcus micros was weak and irregular, and no effects of bacteroides asaccharolyticus were observed despite the use of high doses (10(8) colony-forming unite per ear). ...19827086197
factors determining the occurrence of serum agglutinins to eubacterium and peptostreptococcus species in patients with crohn's disease and ulcerative colitis.recently, the demonstration of serum agglutinins to eubacterium and peptostreptococcus strains has been found to be useful as a diagnostic test for crohn's disease. therefore, conditions determining the occurrence of these antibodies were studied in patients with crohn's disease and ulcerative colitis. localization of crohn's disease in the colon, the presence of fistulae and serum levels of immunoglobulins were found to be contributory determinants for the occurrence of the agglutinins.19827095311
anaerobic bacterial populations on normal and diseased human biopsy tissue obtained at colonoscopy.human epithelium was cultured to characterize differences in microbial populations between regions of normal colon and between polyps, inflammatory bowel disease, and cancer and their respective adjacent normal mucosa. twenty-one patients (12 polyps, 5 inflammatory bowel disease, 4 cancer) underwent colonoscopy with anaerobic culture of mucosal biopsies from normal and diseased ascending, transverse, descending, and sigmoid colon. no differences for total number of organisms and recovery of spec ...19827103479
etiologic factors in acute suppurative thyroiditis. 19827108286
complete amino acid sequence of the 4fe-4s, thermostable ferredoxin from clostridium thermoaceticum.the complete amino acid sequence of the 4fe-4s ferredoxin from the thermophilic bacterium clostridium thermoaceticum has been determined. the protein is extremely thermostable and is the only known clostridial ferredoxin to contain a single [4fe-4s] cluster. the sequence totals 63 residues and includes the first tryptophan (trp-26) reported for a clostridial ferredoxin, and other amino acids not commonly found in clostridial or clostridial-like ferredoxins: methionine (met-1), histidine (his-33) ...19827115670
analysis of short-chain acids from anaerobic bacteria by high-performance liquid chromatography.a standard mixture of 25 short-chain fatty acids was resolved by high-performance liquid chromatography, using an aminex hpx-87 column. the acids produced in culture media by anaerobic bacteria were analyzed by high-performance liquid chromatography after extraction with ether and reextraction into a small volume of 0.1 n naoh. the presence of fumaric acid in culture extracts of peptostreptococcus anaerobius was confirmed by gas chromatography-mass spectrometry analysis of the trapped eluent fra ...19827119103
evaluation of a metronidazole disk test for the presumptive identification of anaerobes.a total of 632 bacterial strains recovered under anaerobic conditions from clinical specimens were tested from their susceptibility to metronidazole by a disk diffusion test using 5 micrograms metronidazole disks. three-hundred-fifty-five of the 632 bacterial strains exhibited susceptibility the metronidazole, and each was determined to be an obligate anaerobe. the remaining 277 isolates showed resistance to the 5 micrograms disk. of these resistant strains, 257 were determined to be facultative ...19827124785
a bacteriological and/or immunological study of anaerobic streptococcal infection in chronic tonsillitis, paratonsillitis and periodontitis patients.anaerobic streptococci were isolated from 100% of tonsil tissue samples obtained from 36 chronic tonsillitis patients. the strains displayed antigenic and other differences. sera of chronic tonsillitis, paratonsillitis and periodontitis patients exhibited significantly more often, and in significantly higher titres, antibodies to the anaerobic streptococci isolated from tonsils than did sera of normal persons.19827142691
direct quantitative determination of acidic end products in clinical specimens for presumptive diagnosis of anaerobic infections.acidic metabolic products determined directly in clinical specimens of 51 anaerobic and 12 aerobic infections by quantitative gas-liquid chromatography (glc) were correlated with the bacteriologic findings. the presence of appreciable amounts of succinic acid, more than 1 microm per ml, propionic and isovaleric acid, at concentrations not exceeding 3 microm per ml, was strong evidence for bacteroides fragilis infections. when isobutyric acid was also detected, a b. melaninogenicus infection coul ...19827149527
endometrial bacteriology in patients with endometrial cancer before and after primary intracavitary irradiation using ir-192 and an afterloading a prospective clinical and bacteriological study the endocervical and endometrial flora of 38 patients with biopsy proven cancer of the endometrium was studied before and after intrauterine irradiation with 10 gy (ir-192, afterloading device, buchler, braunschweig). bacteriological swabs were taken transcervically. anaerobic transport media were used and cultures were done aerobically and anaerobically. the mean number of aerobic organisms per patient increased from 2.13 to 2.49 (not signific ...19827149781
isolation and characterisation of bacteria from pyothorax (empyaemia) in cats. 19827164339
biotransformation of 1-nitropyrene in intestinal anaerobic bacteria.mutagenic nitroaromatic compounds have recently been found in photocopies, urban atmosphere, automobile exhaust and wastewater. 1-nitropyrene (1-np) is readily formed when pyrene, ubiquitous in the environment, is exposed to nitrogen dioxide in the urban atmosphere or in automobile exhaust, and is highly mutagenic, inducing 449 his+ revertants/plate/nmol from salmonella typhimurium strain ta98 in the absence of s9 fraction in the salmonella-microsome test. it is possible to swallow sputum or som ...19827167066
[the effect of tinidazole on the anaerobic bacterial flora in patients undergoing gynecological surgery].the various effects have been examined against the anaerobes by administering tinidazole when the operations of gynecology and obstetrics and the following results were obtained. 1. the serum concentrations after the single administration of 1,000 mg of tdz showed 56.6 micrograms/ml of the peak value (3 hours) and 27.7 micrograms/ml of 24 hours value, and the antibacterial potency was maintained for a long duration. the concentration in the vaginal secretions was 21.8 micrograms/tampon within 24 ...19827176070
response of mice to transtracheal pulmonary inoculation of anaerobic bacteria.the transtracheal inoculation of a mixture of anaerobic bacteria, with or without hydrochloric acid, into the lungs of mice caused nonlethal pneumonia and abscesses in a minority of challenged animals. all animals cleared organisms and recovered by 30 days. secondary infection with escherichia coli was common but did not affect the response to challenge with the anaerobes.19827176528
the assay on a defined medium of the effects of beta-2-thienylalanine on the growth of anaerobic bacterial isolates from phenylketonuric patients.faecal samples were taken from three diet-managed phenylketonuric children to determine effects of beta-2-thienylalanine (beta-2-t) on indigenous bacteria. from sample swabs, 127 anaerobes were identified and tested for beta-2-t inhibition on a phenylalanine (phe)-free medium, anaerobe inhibition test (ait) agar. of the isolates, 77.9% grew sufficiently to assay reactions on at least 25% of ait plates. using phe-containing columbia agar, 86.5% of the strains could be assayed. none of 28 bacteroi ...19807189815
on the formation of 3-phenylpropionate and the different stereo-chemical course of the reduction of cinnamate by clostridium sporogenes and peptostreptococcus anaerobius. 19817202724
animal model for anaerobic lung abscess.there are no satisfactory animal models for the study of anaerobic lung abscess. aspiration of food, gastric mucin, or hydrochloric acid, or any combination of these, along with oropharyngeal bacteria, is commonly believed to cause aspiration pneumonia and lung abscess. in the animal model described, none of the adjuvants was effective in producing anaerobic lung abscesses. anaerobic bacteria derived from dental scrapings of a healthy adult (peptococcus morbillorum, fusobacterium nucleatum, euba ...19817216463
management of an anaerobic infection in a prosthetic knee with long-term antibiotics alone: a case report.a 74-year-old woman had a spherocentric arthroplasty performed on her left knee. after four postoperative weeks, she developed and swelling in the joint. an anaerobic streptococcus was isolated from a joint aspirate. the patient was treated solely with knee immobilization and intravenous penicillin g for five weeks. joint aspirates at the end of treatment and six months posttreatment were sterile. however, after ten posttreatment months, the peptostreptococcal infection recurred. sterile joint a ...19817226607
production of p-hydroxyhydrocinnamic acid from tyrosine by peptostreptococcus anaerobius.peptostreptococcus anaerobius was found to metabolize tyrosine to p-hydroxyhydrocinnamic acid [3-(p-hydroxyphenyl)propionic acid]. this acid was detected in spent growth media by gas-liquid chromatography, and its identity was confirmed by mass spectrometry.19807229012
production of deoxyribonuclease, ribonuclease, coagulase, and hemolysins by anaerobic gram-positive cocci.clinical isolates of peptococcus and peptostreptococcus species and streptococcus intermedius strains were obtained from local hospitals. after confirmed identification, each isolate was tested for the in vitro production of deoxyribonuclease, ribonuclease, coagulase, and hemolysins. of the 60 strains studied, 18 had enzymatic activity. the variability of enzyme production suggests that such assays are not suitable as an aid to identification of these organisms.19817229018
studies of anaerobic bacteria in chronic otitis the 760 cases of chronic otitis media studied from 1970 to 1976, the detection ratio of anaerobic bacteria and the relation between the nature of anaerobic bacteria and the conditions of focus were investigated. in 8.2% of 760 cases 9 kinds and 81 strains of anaerobic bacteria was detected. most of the anaerobic bacteria were peptococcus sp. or bacteroides sp. and in general non-sporogenic anaerobic bacteria accounted for 97.5%. anaerobes were frequently detected in cases where the middle ea ...19817231031
a possible role of eubacterium and peptostreptococcus species in the aetiology of crohn's disease.the faecal flora of patients with crohn's disease has been found to contain higher numbers of anaerobic gram-negative rods and gram-positive coccoid rods than the flora of healthy subjects. in a considerable percentage of sera from patients with crohn's disease, agglutinating antibodies were found against four strains of the coccoid rods, in contrast to sera from patients with ulcerative colitis, other diseases and healthy subjects. in the present study, coccoid strains c18, me46 and me47 were f ...19807235691
characterization of the cecal bacteria of normal hundred ninety-two isolates from cecal contents of three normal weaned pigs were obtained by means of anaerobic roll tube methods and were characterized. seventy-eight percent of the isolates were gram-negative. the most numerous species isolated from each of the pigs was bacteroides ruminicola. this species accounted for 35% of the isolates that were characterized, and selenomonas ruminantium accounted for 21% of the isolates. other gram-negative bacteria isolated from all three pigs were b ...19817235711
agglutination of eubacterium and peptostreptococcus species as a diagnostic test for crohn's disease.the anaerobic fecal flora of patients with crohn's disease has been found to differ from the flora of healthy subjects by higher numbers of anaerobic gram-negative rods and gram-positive coccoid rods. agglutinating antibodies against strains of coccoid rods, belonging to species of eubacterium and peptostreptococcus were detected in much higher percentages of sera from patients with crohn's disease than in those from patients with ulcerative colitis, and healthy subjects. on the basis of the res ...19817250896
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