Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| antibacterial properties of electron beam-sterilized gutta-percha cones. | the purpose of the study was to determine the effect of electron beam sterilization on gutta-percha cones (gpcs) at different times after sterilization. an agar diffusion test was used with -one aerobic bacterium (bacillus subtilis) and five oral anaerobic bacteria (fusobacterium nucleatum, peptostreptococcus micros, porphyromonas gingivalis, propionibacterium acnes, and veillonella parvula). with each microorganism 30 agar plates were prepared, evenly distributed among three groups (group 1: un ... | 2001 | 11487145 |
| the clinical relevance of microbiologic testing: a comparative analysis of microbiologic samples secured from the same sites and cultured in two independent laboratories. | a field study using five different private periodontal practices was conducted; it compared two microbiologic culture samples simultaneously secured from the same sites within 23 individual patients and submitted for bacterial identification and antibiotic sensitivity testing to two separate laboratories. the results from the two laboratories were often different. in no instance did both laboratories agree on the presence of identical bacterial species. when only bacteria above threshold levels ... | 2001 | 11490400 |
| proposal of the genera anaerococcus gen. nov., peptoniphilus gen. nov. and gallicola gen. nov. for members of the genus peptostreptococcus. | members of genus peptostreptococcus have previously been found to be distantly related to the type species, peptostreptococcus anaerobius, on the basis of 16s rdna sequence similarities. they were divided into three major phylogenetic groups, and their peptidoglycan structure and biochemical traits differed between groups. the reclassification of the species of these three groups into three new genera, peptoniphilus gen. nov., anaerococcus gen. nov. and gallicola gen. nov., is proposed. the genu ... | 2001 | 11491354 |
| viable bacteria in root dentinal tubules of teeth with apical periodontitis. | two sets of teeth with apical periodontitis were collected at different geographic locations to study the identity of bacteria left in the root dentinal tubules. root dentin of 20 of these teeth was cultured from three locations between pulp and cementum (a, b, and c). in addition dentin from eight teeth was examined histologically. using the culturing technique bacteria were found in 77% of the dentin samples from set 1 (amsterdam) and in 87.5% of the dentin samples from set 2 (glasgow). at gre ... | 2001 | 11491642 |
| molecular analysis of peptostreptococcus micros isolates from patients with periodontitis. | recent studies provide strong evidence implicating peptostreptococcus micros in the pathogenesis of various oral infections, including oropharyngeal abscesses and periodontal disease. to date, very little is known regarding the role of p. micros in periodontal disease. therefore, a genetic analysis was initiated to differentiate among strains of p. micros infecting periodontal patients. | 2001 | 11495135 |
| molecular identification of microorganisms from endodontic infections. | a relatively wide range of bacteria have been isolated from root canals using standard culture techniques. however, only 50% of the bacteria in the oral cavity are cultivable (s. s. socransky et al., arch. oral biol. 8:278-280, 1963); hence, bacterial diversity in endodontic infections is underestimated. this study used a pcr-based 16s rrna gene assay, followed by cloning and sequencing of 16s rrna amplicons from a small subset of samples to assess the diversity of bacteria present in infected r ... | 2001 | 11526164 |
| [soft tissue infections caused by non-group a streptococci]. | 2001 | 11555914 | |
| bacteriology of adenoids and tonsils in children with recurrent adenotonsillitis. | adenoids and tonsils electively removed from 25 children with a history of recurrent group a beta-hemolytic streptococci (gabhs) adenotonsillitis were cultured for aerobic and anaerobic bacteria. two hundred twenty-four organisms (112 aerobes and facultatives, 110 anaerobes, and 2 candida albicans) were isolated from the tonsils, and 229 (111 aerobes and facultatives and 118 anaerobes) were isolated from the adenoids. mixed infection was present in all instances, with an average of 9.1 isolates ... | 2001 | 11558761 |
| [comparative study of chromatography-mass spectrography of microorganism's chemical markers in blood and intestinal mucosa bioptats]. | fatty acids, that are microorganism's markers in blood and jejunum's, ileum's, and large intestine's bioptats of the patients with irritable bowel syndrome were investigated with gas chromatography. markers of cl. perfringens, cl. difficile, enterococcus, streptomyces, enterobacterial, klebsiella, e. coli, peptostreptococcus, candida albicans, genus of streptococcus, of staphylococcus, of fusobacterium sp and others microorganisms were revealed. settling of intestinal mucosa was the same in jeju ... | 2001 | 11565126 |
| complex between peptostreptococcus magnus protein l and a human antibody reveals structural convergence in the interaction modes of fab binding proteins. | peptostreptococcus magnus protein l (ppl) is a multidomain, bacterial surface protein whose presence correlates with virulence. it consists of up to five homologous immunoglobulin binding domains that interact with the variable (vl) regions of kappa light chains found on two thirds of mammalian antibodies. | 2001 | 11587642 |
| [clinical and microbiological study of adult periodontal disease]. | the aim of this study was to carry out a microbiological evaluation of sites with and without clinical evidence of moderate and severe periodontitis and their correlation with clinical parameters. a total of 52 disease sites and 10 healthy sites were selected according to clinical criteria. the following clinical indexes were measured for all the sites: plaque index, gingival index, blood on probing, depth on probing and insertion level. samples of subgingival plaque were collected for culture a ... | 2001 | 11594003 |
| roles of native topology and chain-length scaling in protein folding: a simulation study with a go-like model. | we perform folding simulations on 18 small proteins with using a simple go-like protein model and analyze the folding rate constants, characteristics of the transition state ensemble, and those of the denatured states in terms of native topology and chain length. near the folding transition temperature, the folding rate k(f) scales as k(f) approximately exp(-c rco n(0.6)) where rco and n are the relative contact order and number of residues, respectively. here the topology rco dependence of the ... | 2001 | 11601854 |
| comparative in vitro activity of ertapenem and 11 other antimicrobial agents against aerobic and anaerobic pathogens isolated from skin and soft tissue animal and human bite wound infections. | we studied the comparative in vitro activity of ertapenem, a new carbapenem, against 240 aerobic and 180 anaerobic recent clinical bite isolates using an agar dilution method and an inoculum of 10(4) cfu/spot for aerobes and 10(5) cfu/spot for anaerobes. ertapenem inhibited 410/420 (98%) of the isolates tested at < or = 4 mg/l with only 4/5 campylobacter gracilis and 1/3 campylobacter rectus strains requiring . or = 16 mg/l for inhibition. ertapenem was only moderately active (mic 8 mg/l) agains ... | 2001 | 11679553 |
| efficacy of manual and powered toothbrushes (ii). effect on microbiological parameters. | the purpose of the present investigation was to determine the effect of self-performed supragingival plaque removal using either manual (crest complete) or power (braun 3d plaque remover) toothbrushing on supra and subgingival plaque composition. | 2001 | 11686813 |
| absence of a specific subgingival microflora in adults with down's syndrome. | periodontal disease in down's syndrome (ds) is generally characterized by a high degree of bone loss. bone loss of 5 mm or more is observed in 70% of these subjects. among ds subjects, considerable differences in disease progression occur. so far, no studies have been conducted in which specific properties of the subgingival microflora have been related to the condition observed. | 2001 | 11686820 |
| calibration of the disk diffusion test for trovafloxacin susceptibility testing of four anaerobic species. | to study trovafloxacin susceptibility among clinical isolates of four anaerobic bacterial species using minimum inhibitory concentrations (mic) determinations, e test assays and disk diffusion test results and to calibrate the disk diffusion method for these species using single strain regression analysis (sra). | 2000 | 11168107 |
| early colonization of dental implants by putative periodontal pathogens in partially edentulous patients. | there is limited scientific information available on the early colonization of the peri-implant pockets in partially edentulous individuals. knowledge about this process is one step in better understanding the etiology and pathogenesis of peri-implantitis. in this study, the early colonization of the peri-implant pockets by putative periodontal pathogens was studied in 20 partially edentulous individuals using anaerobic culture techniques. at baseline, the presence and levels of putative periodo ... | 2000 | 11168244 |
| profile of subgingival microbiota in children with mixed dentition. | a diversity of microbial species has been detected in children's oral flora at an early age. to investigate the composition of the subgingival microbiota of different groups of teeth in children with mixed dentition, 40 systemically healthy children, aged 7-8 years, randomly chosen, were examined. subgingival plaque samples were taken from the mesiobuccal sites of 21, 41, 16 and 36 permanent teeth and 53, 73, 64 and 84 deciduous teeth. the samples were cultured for bacterial isolation anaerobica ... | 2000 | 11155173 |
| detection of major putative periodontopathogens in korean advanced adult periodontitis patients using a nucleic acid-based approach. | although extensive microbial analyses have been performed from subgingival plaque samples of periodontitis patients, systematic analysis of subgingival microbiota has not been carried out in a korean population so far. the purpose of this study was to describe the prevalence of major putative periodontopathogens in korean patients by culture-independent methods. | 2000 | 11022767 |
| differences in the composition of the subgingival microbiota of two periodontitis populations of different geographical origin. a comparison between spain and the netherlands. | the purpose of this study was to compare the subgingival microbiota of two geographically distinct patient populations using identical clinical and bacteriological methods. adult patients with a diagnosis of periodontitis were consecutively selected according to pre-defined clinical criteria. microbiological samples were taken from the deepest four sites with bleeding. the samples were plated on blood agar plates, for the determination of the total anaerobic counts and identification of specific ... | 2000 | 11037754 |
| [bacteria isolated from surgical infections and their susceptibilities to antimicrobial agents. special references to bacteria isolated between april 1997 and march 1998]. | the annual multicenter studies on isolated bacteria from infections in general surgery and their antimicrobial susceptibility have been conducted in 19 facilities in japan since july 1982. this paper describes the results obtained during the period from april 1997 to march 1998. the number of cases investigated as objectives was 215 for one year. a total of 420 strains (170 strains from primary infections and 250 strains from postoperative infections) were isolated from 174 cases (80.9% of total ... | 2000 | 11070817 |
| microbiological parameters associated with il-1 gene polymorphisms in periodontitis patients. | polymorphisms in the cluster of il-1 genes have been significantly associated with the severity of adult periodontitis. the purpose of this study was to compare microbiological parameters in il-1 genotype negative and positive adult subjects with a range of periodontitis severities. | 2000 | 11073323 |
| bacterial susceptibility to amoxicillin and potassium clavulanate in advanced periodontitis patients not responding to mechanical therapy. | between 4 and 8% of periodontitis patients are reported to respond poorly to conventional therapy. in these cases, adjunctive use of systemic antibiotics might be a reasonable therapeutic approach. the purpose of this study was to evaluate the effects of systemic amoxicillin/clavulanate as adjunct to periodontal surgery on the predominant subgingival microorganisms in patients not responding to mechanical therapy. furthermore, the bacterial susceptibility to amoxicillin/clavulanate was analyzed ... | 2000 | 11073328 |
| [successful treatment for descending necrotizing mediastinitis: a case report]. | a 21-year-old female was admitted to our hospital because of high fever, neck swelling, and dyspnea. she was diagnosed as descending necrotizing mediastinitis (dnm) extended from odontogenic infection. on the day of admission, she underwent cervical drainage. next day, the ct scan showed an abscess below the tracheal bifurcation and bilateral pleural effusion. mediastinal drainage was performed through a right thoracotomy, and a left thoracic tube was inserted. anaerobic peptostreptococcus was f ... | 2000 | 11079317 |
| bacteriology in disease: failure of a micro-ecological balance? | both aerobic (mainly alpha-haemolytic streptococci) and anaerobic micro-organisms (like prevotella and peptostreptococcus species) are able to interfere with the growth of potential pathogens such as group a beta-haemolytic streptococci, streptococcus pneumoniae, haemophilus influenzae and moraxella catarrhalis. the present paper reviews the application of this concept of interference with respect to the development of common acute ear-, nose- and throat infections. | 2000 | 11082778 |
| the effects of amoxicillin therapy on skin flora in infants. | in order to determine the effect of amoxicillin therapy on the perineal skin microbial flora in infants, we took quantitative bacterial and fungal cultures of perineal and sternal areas from 25 infants treated with amoxicillin (40 mg/kg/day) for 10 days. specimens were obtained prior to therapy, within 3 days of conclusion of therapy, and 14-16 days later. immediately following therapy, a decline in the number of bacterial isolates occurred on both the perineum (89 to 47) and sternum (84 to 39). ... | 2000 | 11085662 |
| human intestinal bacteria capable of transforming secoisolariciresinol diglucoside to mammalian lignans, enterodiol and enterolactone. | seven metabolites were isolated after anaerobic incubation of secoisolariciresinol diglucoside (1) with a human fecal suspension. they were identified as (-)-secoisolariciresinol (2), 3-demethyl-(-)-secoisolariciresinol (3), 2-(3-hydroxybenzyl)-3-(4-hydroxy-3-methoxybenzyl)butane-1,4-diol (4), didemethylsecoisolariciresinol (5), 2(3-hydroxybenzyl)-3-(3,4-dihydroxybenzyl)butane-1,4-diol (6), enterodiol (7) and enterolactone (8). furthermore, two bacterial strains, peptostreptococcus sp. sdg-1 and ... | 2000 | 11086885 |
| bacteriology of histopathologically defined appendicitis in children. | acute appendicitis is the most common surgical emergency in childhood. however, the pathogenesis and detailed microbiology are obscure. | 2000 | 11099090 |
| apparent culture-negative prosthetic valve endocarditis caused by peptostreptococcus magnus. | in two patients with prosthetic valve endocarditis due to peptostreptococcus magnus, blood cultures in the bact/alert and bactec 9240 systems were signal negative. the capability of the bact/alert system to detect various peptostreptococcus species was assessed. p. magnus and p. anaerobius could not be detected, and subcultures remained negative. the growth in conventional media of these two species and other peptostreptococcus species was similar. | 2000 | 11101611 |
| transgenic rainbow trout expressed sgnrh-antisense rna under the control of sgnrh promoter of atlantic salmon. | a recombinant vector containing antisense dna complementary to atlantic salmon (salmo salar) sgnrh cdna driven by specific promoter pab derived from a corresponding sgnrh gene was introduced into rainbow trout (oncorhynchus mykiss) eggs. this resulted in transgenic animals that had integrated one copy of the transgene into their genome and transmitted it through the germline. antisense-sgnrh mrna (as) was expressed mainly in the brain of transgenic as(+) fish. levels of sgnrh endogenous mrna in ... | 2000 | 11116212 |
| microbiology of healthy and diseased adenoids. | to determine the qualitative and quantitative microbiology of core adenoid tissue obtained from four groups of 15 children each, with recurrent otitis media (rom), recurrent adenotonsillitis (rat), obstructive adenoid hypertrophy (oah), and occlusion or speech abnormalities (controls). | 2000 | 10852520 |
| evaluation of a phenotypic scheme for identification of the 'butyrate-producing' peptostreptococcus species. | gram-positive anaerobic cocci (gpac) are isolated from approximately one quarter of all infections involving anaerobic bacteria. however, studies of the significance of this group of pathogens have been hindered by an inadequate taxonomy and the lack of a valid identification scheme. in the present study, a phenotypic scheme for the identification of 'butyrate-producing' gpac based on the analysis of volatile fatty acid profiles by gas-liquid chromatography, biochemical profiles (including the u ... | 2000 | 10933261 |
| [the treatment of bacterial vaginosis with dalacin v cream]. | to asses the efficiency of the 2% clindamycin phosphate creme given in 3 and 7 daily courses officially designer for the treatment of bacterial vaginosis (bv). | 2000 | 10948619 |
| comparative in vitro activities of abt-773 against aerobic and anaerobic pathogens isolated from skin and soft-tissue animal and human bite wound infections. | we studied the comparative in vitro activities of abt-773, a new ketolide, against 268 aerobic and 148 anaerobic recent isolates from clinical bites using an agar dilution method and inocula of 10(4) cfu/spot for aerobes and 10(5) cfu for anaerobes. the following are the mic ranges and mics at which 90% of isolates are inhibited (mic(90)s) of abt-773 for various isolates, respectively: pasteurella multocida and pasteurella septica, 0.125 to 2 and 1 microg/ml; other pasteurella species, 0.125 to ... | 2000 | 10952607 |
| aerobic and anaerobic microbiology in intra-abdominal infections associated with diverticulitis. | the aerobic and anaerobic microbiology of intra-abdominal infections associated with diverticulitis was studied in 110 specimens from the peritoneal cavity after intestinal perforation and in 22 specimens from abdominal abscesses. anaerobic bacteria only were isolated from 17 (15%) of the peritoneal specimens, aerobic bacteria only from 12 (11%) and mixed aerobic and anaerobic flora from 81 (74%). a total of 339 bacterial isolates was detected in peritoneal cultures (3.1 per specimen), comprisin ... | 2000 | 10966232 |
| in vitro activity of an evernimicin derivative, sch27899, against anaerobic bacteria and propionibacterium acnes. | the in vitro activity of sch27899, a novel oligosaccharide antimicrobial agent, was compared with those of representatives of six classes of antimicrobial agents (piperacillin, clarithromycin, clindamycin, vancomycin, sitafloxacin and metronidazole) against clinical isolates of anaerobic bacteria and propionibacterium acnes. against peptostreptococcus: spp. and clostridium difficile, sch27899 was the most potent (mic(90) < 0.125 mg/l) of the agents examined. besides these gram-positive anaerobes ... | 2000 | 10980176 |
| in vitro and in vivo antibacterial activities of a new injectable carbapenem, s-4661, against gynaecological pathogens. | carbapenems are often used to treat infections in obstetrics and gynaecology because of their activity against anaerobes. s-4661, a new injectable carbapenem antibiotic, has favourable pharmacokinetic properties and is not hydrolysed by dehydropeptidase i. we investigated the in vitro and in vivo antibacterial activities of s-4661 against strains of streptococcus agalactiae, escherichia coli, peptostreptococcus magnus, bacteroides fragilis and prevotella bivia, which are major pathogens in the f ... | 2000 | 10980177 |
| characterization of peptostreptococcus species in skin infections. | we examined the characteristics of peptostreptococcus species in infectious skin diseases. p. magnus was the species identified most frequently, followed by p. asaccharolyticus. peptostreptococcus species were mainly isolated from infected atheroma and secondary infections due to ulcers; their resistance to five antimicrobial agents was generally low. the resistance of the three predominant peptostreptococcus species to the antimicrobials was similar to that of all of the peptostreptococcus spec ... | 2000 | 10983864 |
| laboratory diagnosis of anaerobic pleuropulmonary infections. | anaerobic organisms play a major role in pleuropulmonary infections. clinical manifestations range from simple aspiration to acute, severe, necrotizing pneumonias to chronic infections, such as lung abscess and empyema. implicated pathogens are usually of endogenous origin. laboratory diagnosis of anaerobic pleuropulmonary infections is based on recovering the etiological agent from clinical specimens. appropriate specimens include pleuml fluid, transtracheal aspirates, transthoracic aspirates a ... | 2000 | 10983929 |
| comparative in vitro activities of gar-936 against aerobic and anaerobic animal and human bite wound pathogens. | gar-936 is a new semisynthetic glycylcycline with a broad antibacterial spectrum, including tetracycline-resistant strains. the in vitro activities of gar-936, minocycline, doxycycline, tetracycline, moxifloxacin, penicillin g, and erythromycin were determined by agar dilution methods against 268 aerobic and 148 anaerobic strains of bacteria (including pasteurella, eikenella, moraxella, bergeyella, neisseria, ef-4, bacteroides, prevotella, porphyromonas, fusobacterium, staphylococcus, streptococ ... | 2000 | 10991855 |
| bacterial immunoglobulin superantigen proteins a and l activate human heart mast cells by interacting with immunoglobulin e. | human heart mast cells (hhmc) have been identified in heart tissue, perivascularly, and in the intima of coronary arteries. in vitro activation of isolated hhmc induces the release of vasoactive and proinflammatory mediators (histamine, tryptase, and cysteinyl leukotriene c(4) [ltc(4)]). we investigated the effects of several bacterial proteins on hhmc activation in vitro. hhmc released histamine, tryptase, and ltc(4) in response to staphylococcus aureus cowan 1 and the immunoglobulin (ig)-bindi ... | 2000 | 10992448 |
| b-cell deficiency predisposes mice to disseminating anaerobic infections: protection by passive antibody transfer. | we have previously demonstrated that a high proportion of rag-2 scid knockout mice, which lack t and b cells, develop orofacial abscesses and disseminated infections following pulpal infection, whereas immunocompetent control mice do not. in the present study, we sought to identify the components of the adaptive immune response which contribute to protection against disseminating anaerobic infections and sepsis. for this purpose, various genetically engineered immunodeficient mice were employed, ... | 2000 | 10992465 |
| [tracheopulmonary-subcutaneous fistula associated with anaerobic subcutaneous abscess on mechanical ventilator support in a patient with duchenne muscular dystrophy]. | a 29-year-old japanese man with duchenne muscular dystrophy was placed on a mechanical ventilator support at 23 years of age and admitted to our hospital at 25 years of age. he had severe neck contracture deviated to the left side which resulted in dysphagia and microaspiration. at 29 years of age, he developed left lobar pneumonia accompanied by slight fever, back pain and a foul odor from the patient's sputum. although the patient received broad spectrum antibiotics, pneumonia disseminated to ... | 2000 | 11002735 |
| peptostreptococcus micros coaggregates with fusobacterium nucleatum and non-encapsulated porphyromonas gingivalis. | coaggregation is one of the potential colonization strategies of oral microorganisms, often involving fimbrial structures in the interactions. in this study, the coaggregation characteristics of the rough and smooth genotypes of the periodontal pathogen peptostreptococcus micros were compared to investigate the role of the fibril-like structures of the rough genotype in coaggregation. of the 11 oral species tested, only fusobacterium nucleatum strains and non-encapsulated porphyromonas gingivali ... | 2000 | 10612731 |
| evaluation of the oxyrase oxyplate anaerobe incubation system. | the oxyrase oxyplate anaerobe incubation system was evaluated for its ability to support the growth of clinically significant anaerobic bacteria previously identified by the anaerobe reference laboratory at the centers for disease control and prevention. the results were compared with those obtained with conventional anaerobe blood agar plates incubated in an anaerobe chamber. we tested 251 anaerobic bacterial strains. plates were read at 24, 48, and 72 h; growth was scored by a numerical coding ... | 2000 | 10655335 |
| in vitro and in vivo antibacterial activities of biapenem in the fields of obstetrics and gynecology. | biapenem is a new injectable carbapenem antibiotic which has favorable pharmacokinetic properties, and is stable to hydrolysis by dehydropeptidase i. biapenem inhibited more than 90% of clinical isolates of streptococcus agalactiae, escherichia coli, peptostreptococcus magnus, bacteroides fragilis and prevotella bivia at the concentration of 3.13 mg/l. the mic(90) of biapenem against pseudomonas aeruginosa was lower than that of panipenem, equivalent to that of imipenem, and greater than that of ... | 2000 | 10671758 |
| past administration of beta-lactam antibiotics and increase in the emergence of beta-lactamase-producing bacteria in patients with orofacial odontogenic infections. | the purpose of this study was to determine the current status of beta-lactamase-producing bacteria in orofacial odontogenic infections. | 2000 | 10673654 |
| [the dynamics of the colonization by the microbial flora of the mouth of the different materials used for dental prosthetics]. | colonization of materials used for making dentures with oral microflora was studied in 12 patients with chronic generalized medium severe periodontitis during remission. time course of dentures colonization with representatives of oral microflora (s. sanguis, peptostreptococcus sp., prevotella oralis) and with bacteria pathogenic for the periodontium (prevotella melaninogenica, a. naeslundii, fusobacterium sp.) depends on the material from which the dentures are made. minimal colonization of bac ... | 2000 | 10693351 |
| lack of antimicrobial effect on periodontopathic bacteria by ultrasonic and sonic scalers in vitro. | the purpose of this study was to assess the antimicrobial effects of a sonic and ultrasonic scaler generally used for subgingival scaling on gram-negative and gram-positive periodontopathic bacteria. | 2000 | 10703657 |
| peptostreptococcus micros smooth and rough genotypes in periodontitis and gingivitis. | two genotypes can be distinguished within the species peptostreptococcus micros: a smooth (sm) and a rough (rg) type. to date no systematic study has been performed on the prevalence and proportion of both types in untreated periodontitis patients and subjects without destructive periodontal disease. therefore, the present study was performed to investigate: 1) the relative importance of the sm and the rg genotype of p micros in periodontitis and gingivitis; 2) the correlation between smoking an ... | 2000 | 10711611 |
| a case of paraspinal abscess and diskitis due to peptostreptococcus micros. | 2000 | 10722468 | |
| crystallization and preliminary x-ray diffraction studies of mutants of b1 igg-binding domain of protein l from peptostreptococcus magnus. | the small 62-residue igg-binding domain b1 of protein l from peptostreptococcus magnus (ppl-b1) has proven to be a simple system for the study of the thermodynamics and kinetics of protein folding. x-ray diffraction studies have been initiated in order to determine how the thermostability, folding and unfolding rates of a series of point mutations spanning ppl-b1 correlate with the high-resolution structures. to this end, a tryptophan-containing variant of ppl-b1 (herein known as wild type) and ... | 2000 | 10739935 |
| expression of a heterologous glutamate dehydrogenase gene in lactococcus lactis highly improves the conversion of amino acids to aroma compounds. | the first step of amino acid degradation in lactococci is a transamination, which requires an alpha-keto acid as the amino group acceptor. we have previously shown that the level of available alpha-keto acid in semihard cheese is the first limiting factor for conversion of amino acids to aroma compounds, since aroma formation is greatly enhanced by adding alpha-ketoglutarate to cheese curd. in this study we introduced a heterologous catabolic glutamate dehydrogenase (gdh) gene into lactococcus l ... | 2000 | 10742211 |
| aerobic and anaerobic microbiology of wound infection following spinal fusion in children. | to study the aerobic and anaerobic microbiology of wound infections following spinal fusion in children. | 2000 | 10765134 |
| an experimental mouse model to study the pathogenicity of prevotella bivia and investigations of possible virulence. | induction of subcutaneous abscesses in mice was used to study the pathogenicity of prevotella bivia both in mono-infection and in mixed cultures with escherichia coli and peptostreptococcus spp. virulence factors such as coaggregation and aggregate formation of cells, haemagglutination activity and tolerance to serum bactericidal activity were investigated for their possible role in p bivia pathogenicity. monocultures of p bivia, e coli and peptostreptococcus spp did not induce subcutaneous absc ... | 2000 | 10786446 |
| profile of subgingival microbiota in children with primary dentition. | eruption of primary teeth has a great influence on the oral environment by providing suitable niches for bacterial colonization. the aim of the study was to investigate the composition of the subgingival microbiota of primary incisors, canines and molars in 40 systemically healthy children aged 4-5 yr, chosen randomly. subgingival plaque samples were taken from the mesiobuccal sites of primary incisors (61, 81), canines (53, 73) and molars (64, 84). the samples were cultured for bacterial isolat ... | 2000 | 10791707 |
| dynamics of nasopharyngitis in children. | our goal was to characterize the dynamics and bacterial interaction of the aerobic and anaerobic flora of nasal discharge of children at different stages of uncomplicated nasopharyngitis. methods and patients: serial semiquantitative nasopharyngeal (np) and quantitative nasal discharge (nd) cultures were taken every 3 to 5 days from 20 children in whom purulent discharge eventually developed (group 1), and a single culture was obtained from a group of 20 who had only clear discharge (group 2). | 2000 | 10793349 |
| comparative activity of moxifloxacin in vitro against obligately anaerobic bacteria. | the antimicrobial activity of moxifloxacin and seven other antibiotics (four of them quinolones) against 292 strains of obligately anaerobic bacteria was assessed employing a broth microdilution technique performed in wilkens-chalgren broth. mic50/mic90 values (mg/l) for moxifloxacin were as follows: bacteroides fragilis (n = 62) 0.25/2, bacteroides ovatus (n = 70) 1/4, bacteroides vulgatus (n = 29) 0.25/1, bacteroides thetaiotaomicron (n = 17) 2/2, bacteroides caccae (n = 11) 1/2, prevotella sp ... | 2000 | 10795599 |
| synergic activity, for anaerobes, of trovafloxacin with clindamycin or metronidazole: chequerboard and time-kill methods. | chequerboard titrations were used to test the activity of trovafloxacin, alone and in combination with clindamycin or metronidazole, against 156 gram-positive or gram-negative anaerobes, including 47 bacteroides fragilis group, 36 prevotella spp., 26 fusobacteria, 21 peptostreptococci and 26 clostridia. mic50/mic90 values (mg/l) of each drug alone against all 156 strains were: trovafloxacin, 0.5/1; clindamycin, 0.25/2; metronidazole, 1/2. synergy (fic indices </= 0.5) was seen in two strains wit ... | 2000 | 10797085 |
| translocation of indigenous microflora in an experimental model of sepsis. | translocation of viable bacteria from gut to bloodstream and other sterile body sites during shock has been demonstrated in several experimental and clinical studies. the factors causing translocation and its incidence at different stages of shock are not known. the aim of the study was to evaluate the importance of several factors causing translocation of indigenous microflora in an experimental model of septic shock based on intraperitoneal escherichia coli sepsis in rats. counts of inoculated ... | 2000 | 10798556 |
| a breakdown of symmetry in the folding transition state of protein l. | the 62 residue igg binding domain of protein l consists of a central alpha-helix packed on a four-stranded beta-sheet formed by n and c-terminal beta-hairpins. the overall topology of the protein is quite symmetric: the beta-hairpins have similar lengths and make very similar interactions with the central helix. characterization of the effects of 70 point mutations distributed throughout the protein on the kinetics of folding and unfolding reveals that this symmetry is completely broken during f ... | 2000 | 10801362 |
| linezolid. | linezolid is an oxazolidinone antibacterial agent that acts by inhibiting the initiation of bacterial protein synthesis. cross-resistance between linezolid and other inhibitors of protein synthesis has not been demonstrated. linezolid has a wide spectrum of activity against gram-positive organisms including methicillin-resistant staphylococci, penicillin-resistant pneumococci and vancomycin-resistant enterococcus faecalis and e. faecium. anerobes such as clostridium spp., peptostreptococcus spp. ... | 2000 | 10804037 |
| [otogenic cerebral abscess: a persistent problem]. | a young man with otitis media and cholesteatoma of the left ear developed secondary cerebral abscess. the clinical debut was non-specific, with headache, mild fever, and mild persistent otalgia in spite of early antibiotic treatment. studies revealed a cerebral abscess, so ent surgery in collaboration with the neurosurgery department was decided. this case illustrates that clinical manifestations in such cases can be mild and highlights the need to exclude this type of serious pathology. | 2000 | 10804119 |
| in vitro inhibition of commercial douche products against vaginal microflora. | recently, vaginal douching has been associated with many health risks in women. the aim of this study was to analyze the effect of commercial douche products against various vaginal microorganisms, including lactobacilli. seven commercial douches were tested against eight lactobacillus clinical isolates and three type strains from the american type culture collection. bv-associated bacteria included six strains of five genera: gardnerella, mobiluncus, mycoplasma, peptostreptococcus, and ureaplas ... | 2000 | 10805365 |
| microbiology of infected poison ivy dermatitis. | we report the aerobic and anaerobic microbiology of secondarily infected poison ivy dermatitis. the study involved retrospective review of clinical and microbiology laboratory records of patients with secondarily infected poison ivy lesions. bacterial growth was noted in 33 specimens. aerobic or facultative anaerobic bacteria only were present in 18 (55%) patients, anaerobic bacteria only in seven (21%), and mixed anaerobic-aerobic bacteria in eight (24%). forty-five isolates were recovered (1.4 ... | 2000 | 10809852 |
| [what is your diagnosis? bilateral abscess forming pneumonia]. | 2000 | 10815462 | |
| strategy for 3-d computed tomography diagnosis and treatment of small pulmonary arteriovenous fistula. | in a patient with left hemianopsia, computed tomography (ct) and magnetic resonance imaging revealed an intracranial space-occupying lesion in the right occipital region. a cerebral abscess was removed at craniotomy. enhanced pulmonary ct showed a small coin lesion in the peripheral lingula. and at 3-d ct two nidi with feeding arteries and draining veins were seen, indicating pulmonary arteriovenous fistula. lingular segmentectomy was performed. | 2000 | 10816068 |
| induction of human immunodeficiency virus type 1 expression by anaerobes associated with bacterial vaginosis. | bacterial vaginosis (bv) is a common disorder characterized by increased levels of anaerobic bacteria in the genital tract. bv has been associated with an increased rate of sexual transmission of human immunodeficiency virus (hiv). the effects of bv-associated anaerobic bacteria on hiv expression in monocytoid cells and t cells were examined. peptostreptococcus asaccharolyticus and prevotella bivia stimulated hiv expression in monocytoid cells, whereas bacteroides ureolyticus, peptostreptococcus ... | 2000 | 10823756 |
| review of the in vitro activity of gemifloxacin against gram-positive and gram-negative anaerobic pathogens. | published reports on the in vitro activity of gemifloxacin mesylate (sb 265805), a new fluoronaphthyridone, against anaerobic pathogens are reviewed here. the studies used a variety of media, inocula and antimicrobial agents. using a proposed breakpoint of 0.5 mg/l, these studies showed that gemifloxacin had generally higher potency against gram-positive anaerobes (clostridium perfringens, all peptostreptococcus spp.) and fusobacteria (fusobacterium nucleatum, fusobacterium necrophorum) and mode ... | 2000 | 10824034 |
| aerobic and anaerobic bacteriology of concurrent chronic otitis media with effusion and chronic sinusitis in children. | to correlate the aerobic and anaerobic microbiologic findings of concurrent chronic otitis media with effusion and chronic maxillary sinusitis. | 2000 | 10680868 |
| postantibiotic effects and bactericidal activities of clarithromycin-14-hydroxy-clarithromycin, versus those of amoxicillin-clavulanate, against anaerobes. | the bactericidal activities and postantibiotic effects (pae) of clarithromycin-14-hydroxy-clarithromycin and amoxicillin-clavulanate against bacteroides fragilis and peptostreptococcus anaerobius were determined. a concentration of twice the mic resulted in bactericidal activity against four of four and three of four organisms at 24 h with clarithromycin-14-hydroxy-clarithromycin and amoxicillin-clavulanate, respectively. the pae of clarithromycin-14-hydroxy-clarithromycin was 1.44 to 3.20 h, co ... | 2000 | 10681358 |
| features of severe periodontal disease in a teenager with chédiak-higashi syndrome. | chédiak-higashi syndrome (c-hs) is a rare congenital disease characterized by defective neutrophil function with abnormal lysosomal inclusions, neutropenia, and reduced chemotaxis. the complete syndrome includes oculocutaneous albinism with photophobia, neurologic features, recurrent infections, and enterocolitis. | 2000 | 10872965 |
| ulcus vulvae acutum. | an acute vulvar ulcer was diagnosed based on a case history of a 17-year-old girl with genital ulcers. no relevant causative factors could be established. the gangrenous form of the disease, cutaneous anergy, and the cultured anaerobic pathogens suggested the possibility of an infectious pathogenesis. | 2000 | 10879308 |
| in vitro activity of telithromycin (hmr 3647) and seven other antimicrobial agents against anaerobic bacteria. | we assessed the in vitro activity of telithromycin (hmr 3647) and seven other antimicrobials against 292 strains of obligately anaerobic bacteria. mics were determined with the microdilution technique and wilkins-chalgren broth according to din 58940-83. mic50/mic90s (mg/l) for telithromycin were 4/4 for bacteroides fragilis, bacteroides ovatus and bacteroides thetaiotaomicron, 2/4 for fusobacterium spp. and bilophila wadsworthia, 2/2 for bacteroides caccae, 1/4 for bacteroides vulgatus, 0.25/4 ... | 2000 | 10882699 |
| the periodontal abscess (i). clinical and microbiological findings. | little information is available regarding the diagnosis and microbiology of periodontal abscesses. the aim of this descriptive clinical and microbiological study was to provide more information in order to help in the characterisation of the periodontal abscess associated to periodontitis. | 2000 | 10883867 |
| [cerebral abscess in an immunocompetent patient]. | 2000 | 10893748 | |
| in vitro activities of mk-826 (l-749,345) against 363 strains of anaerobic bacteria. | the activity of mk-826 was compared to the activities of cefoxitin, ceftriaxone, imipenem, and meropenem against 363 gram-negative and gram-positive anaerobes by using nccls procedures. at least 98% of the strains were susceptible to the carbapenems. all strains of clostridium perfringens, fusobacterium nucleatum, peptostreptococcus, and sutterella wadsworthensis were susceptible to all agents tested. | 2000 | 10898709 |
| toll-like receptor 4-deficient mice have reduced bone destruction following mixed anaerobic infection. | c3h/hej mice have an impaired ability to respond to lipopolysaccharide (lps) due to a mutation in the gene that encodes toll-like receptor 4 (tlr4). the effect of tlr4 deficiency on host responses to endodontic infections is unknown. in the present study, we compared periapical bone destruction, sepsis, and inflammatory cytokine production in lps-hyporesponsive c3h/hej and wild-type control c3h/heouj mice. the mandibular first molars of both strains were subjected to pulpal exposure and infectio ... | 2000 | 10899873 |
| interleukin-1 receptor signaling rather than that of tumor necrosis factor is critical in protecting the host from the severe consequences of a polymicrobe anaerobic infection. | infection of the dental pulp leads to an osteolytic lesion that results from a polymicrobial infection consisting largely of pathogenic anaerobes. infection causes significant morbidity and mortality mediated by bacterial factors and in some cases by the up-regulation of inflammatory cytokines. the inflammatory cytokines interleukin-1 (il-1) and tumor necrosis factor (tnf), in particular, play a complex and central role in the responses to microbial pathogens. however, relatively little is known ... | 2000 | 10899881 |
| characterization of bacterial orofacial infections using a new murine model. | we devised a new murine orofacial infection model using bacteria from odontogenic infection origins and characterized the experimental infections. in this model, bacteria were injected into the submandible of mice. streptococcus constellatus and peptostreptococcus micros produced a single abscess at the injection site and their abscess-forming and lethal abilities were low: the median abscess-forming dose (af(50)) of s. constellatus and p. micros were 10(8.5-10.7)and 10(10.2-10.6)cfu/mouse, and ... | 2000 | 10906266 |
| "checkerboard" assessments of periodontal microbiota and serum antibody responses: a case-control study. | we explored the association between subgingival microbial profiles and serum igg responses to periodontal microbiota in relation to clinical periodontal status. | 2000 | 10914791 |
| [bacterial vaginosis. epidemiology and risk factors]. | bacterial vaginosis (bv) is a change in vaginal ecosystem where lactobacilli dominate, flora is absent or greatly reduced, and replaced with a mixed, predominantly anaerobic flora, consisting of gardnerella vaginalis, mycoplasma hominis, mobiluncus spp, bacteroides spp, prevotela spp, peptostreptococcus spp, fusobacterium spp and porphyromonas spp. the concentration of bacteria increases from 100 to 1000 fold in women with bv compared to healthy women. bv has been formerly known as nonspecific v ... | 2000 | 10916461 |
| in vitro bacterial interference in the nasopharynx of otitis media-prone and non-otitis media-prone children. | to compare the frequency of recovery of potential pathogens and aerobic- and anaerobic-interfering bacteria in the nasopharynx of otitis media-prone (omp) with that in non-omp (n-omp) children. | 2000 | 10922236 |
| critical role of beta-hairpin formation in protein g folding. | comparison of the folding mechanisms of proteins with similar structures but very different sequences can provide fundamental insights into the determinants of protein folding mechanisms. despite very little sequence similarity, the approximately 60 residue igg binding domains of protein g and protein l both consist of a single helix packed against a four-stranded sheet formed by two symmetrically disposed beta-hairpins. we demonstrate that, as in the case of protein l, one of the two beta-turns ... | 2000 | 10932252 |
| the consequences of suppression of anaerobic bacteria. | anaerobic bacteria such as bacteroides fragilis, peptostreptococcus species, and fusobacterium species, when accompanied by aerobic bacteria or in the presence of dead tissue, can cause severe infections. this article discusses the most common type of anaerobic infection, i.e., infection after colonic contamination of the abdominal cavity and soft tissues. colonic anaerobes rarely cause infections as solitary pathogens. mixed infections of aerobes and anaerobes are treated by source control, sur ... | 2000 | 12594909 |
| linezolid: a new antibiotic. | the u.s. food and drug administration recently approved linezolid for the treatment of patients with methicillin-resistant staphylococcal and vancomycin-resistant enterococcal infections. this oxazolidinone antibacterial agent represents the first approved antibiotic of a new structural class in 35 years. linezolid is a synthetic compound that acts by inhibiting the initiation complex formation in bacterial protein synthesis, a mechanism of action distinct from other commercially available antib ... | 2000 | 12847568 |
| intraabdominal abscess managed successfully via the laparoscopic approach. | a rare complication of laparoscopic fundoplication-an intraabdominal abscess located between the fundus and the caudate lobe of the liver-is described. a 41-year-old man had undergone a laparoscopic nissen-rossetti fundoplication for longstanding gastroesophageal reflux disease. on the 5th postoperative day, the patient's general condition became worse, and he developed intermittent-remittent fever (40 degrees c), an elevated white blood cell count (wbc), and an accelerated sedimentation rate. e ... | 2000 | 11265065 |
| growth inhibitory activity of gutta-percha points containing root canal medications on common endodontic bacterial pathogens as determined by an optimized quantitative in vitro assay. | gutta-percha points containing calcium hydroxide, zinc oxide (zno), a mixture of zno and chlorhexidine (zno/chx), iodine-polyvinylpyrrolidone (zno/j-pvp), or a mixture of chx and j-pvp and zno (zno/chx/j-pvp) were tested for their ability to inhibit growth of pure cultures of bacterial species commonly involved in endodontic infections (peptostreptococcus micros, streptococcus intermedius, enterococcus faecalis, and porphyromonas gingivalis). to quantitate growth inhibition, an in vitro assay wa ... | 2000 | 11199764 |
| molecular epidemiology and association of putative pathogens in root canal infection. | the purpose of this study was to investigate the epidemiology of putative pathogens in root canals with apical periodontitis and to determine the associations among the putative pathogens. eighteen symptomatic and 20 asymptomatic teeth from 36 subjects were studied. this research was performed with polymerase chain reaction and hybridization using rrna-based oligonucleotide probes. the most frequently found species was fusobacterium sp. (68.4%), followed by peptostreptococcus micros (44.7%) and ... | 2000 | 11199802 |
| [the influence of anaerobic bacteria and mycoplasma isolated from the genital tract on mast cell activation]. | nowadays, it is known that mast cells, numerously appearing in all organs and being a source of a wide range of mediators and cytokines, are involved both in physiological and pathological processes. the aim of our study was to examine whether vaginal bacteria, especially those participating in bacterial vaginosis, are able to activate mast cells to mediators secretion. the study was done on rat peritoneal mast cells. the mast cells were incubated in vitro with suspensions of bacteroides capillo ... | 2000 | 11286181 |
| the effect of statherin and its shortened analogues on anaerobic bacteria isolated from the oral cavity. | the susceptibility (mic) of 44 strains of anaerobic bacteria isolated from the oral cavity and 3 standard strains to statherin and its c-terminal fragments with sequences qyqqytf, yqqytf, qqytf, qytf and ytf was determined by means of plate dilution technique in brucella agar with 5% content of defibrinated sheep's blood, menadione and hemin. the culture was anaerobic. as shown, at concentrations from 12.5 to 100 microg/ml statherin and its c-terminal fragments inhibited the growth of anaerobic ... | 2000 | 11293657 |
| comparison of microbial composition in the subgingival plaque of adult crowded versus non-crowded dental regions. | it has been reported in the literature that certain species of bacteria (periodontopathogens) present in the subgingival plaque are associated with destructive periodontal disease. the purpose of this study was to investigate and compare the presence and proportional distribution of periodontopathogens in the subgingival plaque of adult crowded versus non-crowded dental regions. thirty adult patients with anterior dental crowding were selected from the orthodontic clinic of the university of pen ... | 2000 | 11307190 |
| [anaerobic infection and its rapid detection in surgical patients]. | to investigate anaerobic infection and its rapid detection in surgical patients. | 1999 | 11829950 |
| [anaerobic bacterial analysis of 436 specimens of clinical infection]. | this study was aimed at the relationship between anaerobes and clinical infection. aerobic and anaerobic bacterial culture was performed for 436 specimens of clinical infection. the incidence of anaerobic bacteria isolates and predominant species were analyzed. the results showed that the incidence of anaerobic isolates was 88.3% (385/436), the main predeminoue species were gram negative nonsporeforming anaerobic rods (bacteroids, prevotella and porphyromonas) and peptostreptococcus. the endogen ... | 1999 | 12205907 |
| [bacterial pneumonia in hiv-infected patients] | patients and methods: this is a retrospective study performed on hiv-positive patients discharged from our institution from january 1993 through december 1998 with a diagnosis of bacterial pneumonia. cases of tb or atypical micobacterial infection were excluded from this analysis. causative organisms were identified, when possible, by taking into account positive cultures from diverse sources (blood, sputum, pleural fluid and others). results: in the 6-yr period we considered, 120 patients were ... | 1999 | 12748443 |
| bacterial vaginosis a "broad overview". | bacterial vaginosis (bv) was first reported in 1995 by gardner and dukes, who described the unique clinical signs and symptoms and the distinctive nature of the vaginal discharge associated with it. they also described a "new" causative organism, which they named "haemophilus vaginalis", subsequently renamed gardnerella vaginalis. bv is currently the most prevalent cause of infectious vaginitis among women attending for genitourinary diseases. bv has a complex microbiology. lactobacillus populat ... | 1999 | 10932748 |
| proteolysis and utilization of albumin by enrichment cultures of subgingival microbiota. | subgingival dental plaque consists mainly of microorganisms that derive their energy from amino acid fermentation. their nutrient requirements are met by the subgingival proteolytic system, which includes proteases from microorganism and inflammatory cells, and substrate proteins from sulcus exudate, including albumin. to determine the selective effect of individual proteins on microbiota, we used albumin as the main substrate for growth. eight subgingval plaque samples from untreated periodonta ... | 1999 | 10895689 |
| bacterial interference in the nasopharynx and nasal cavity of sinusitis prone and non-sinusitis prone children. | the aim of this study was to compare the frequency of recovery of potential pathogens and aerobic and anaerobic interfering bacteria in the nasopharynx and nasal cavity of sinusitis prone (sp) children, with their recovery in non-sinusitis prone (n-sp) children. nasopharyngeal and nasal cultures were taken from 20 sp and 20 n-sp children. potential pathogens and aerobic and anaerobic bacteria with interfering capabilities against these micro-organisms were identified. twenty-one potential pathog ... | 1999 | 10687943 |
| [in vitro activities of levofloxacin and other antibiotics against fresh clinical isolates]. | in this study, the in vitro activity of levofloxacin (lvfx) against 1,020 fresh bacterial clinical isolates was compared with the activities of a range of ofloxacin, ciprofloxacin (cpfx), ampicillin (abpc), cefaclor, cefpodoxime, methicillin and benzylpenicillin. the clinical isolates except vibrio cholerae were collected in japan during 1998 from patients with infectious diseases. mics were determined using the agar dilution method according to the recommendations by the japan society of chemot ... | 1999 | 10746192 |
| [the clinical picture, treatment and prognosis of meningitis due to anaerobic and nonfermentative bacteria]. | the study of incidence, clinical manifestations and prognosis of meningitis with anaerobic and non-fermentative bacteria. | 1999 | 10756944 |