PMID(sorted descending)
efficacy of manual and powered toothbrushes (ii). effect on microbiological parameters.the purpose of the present investigation was to determine the effect of self-performed supragingival plaque removal using either manual (crest complete) or power (braun 3d plaque remover) toothbrushing on supra and subgingival plaque composition.200111686813
comparative in vitro activity of ertapenem and 11 other antimicrobial agents against aerobic and anaerobic pathogens isolated from skin and soft tissue animal and human bite wound infections.we studied the comparative in vitro activity of ertapenem, a new carbapenem, against 240 aerobic and 180 anaerobic recent clinical bite isolates using an agar dilution method and an inoculum of 10(4) cfu/spot for aerobes and 10(5) cfu/spot for anaerobes. ertapenem inhibited 410/420 (98%) of the isolates tested at < or = 4 mg/l with only 4/5 campylobacter gracilis and 1/3 campylobacter rectus strains requiring . or = 16 mg/l for inhibition. ertapenem was only moderately active (mic 8 mg/l) agains ...200111679553
roles of native topology and chain-length scaling in protein folding: a simulation study with a go-like model.we perform folding simulations on 18 small proteins with using a simple go-like protein model and analyze the folding rate constants, characteristics of the transition state ensemble, and those of the denatured states in terms of native topology and chain length. near the folding transition temperature, the folding rate k(f) scales as k(f) approximately exp(-c rco n(0.6)) where rco and n are the relative contact order and number of residues, respectively. here the topology rco dependence of the ...200111601854
[clinical and microbiological study of adult periodontal disease].the aim of this study was to carry out a microbiological evaluation of sites with and without clinical evidence of moderate and severe periodontitis and their correlation with clinical parameters. a total of 52 disease sites and 10 healthy sites were selected according to clinical criteria. the following clinical indexes were measured for all the sites: plaque index, gingival index, blood on probing, depth on probing and insertion level. samples of subgingival plaque were collected for culture a ...200111594003
complex between peptostreptococcus magnus protein l and a human antibody reveals structural convergence in the interaction modes of fab binding proteins.peptostreptococcus magnus protein l (ppl) is a multidomain, bacterial surface protein whose presence correlates with virulence. it consists of up to five homologous immunoglobulin binding domains that interact with the variable (vl) regions of kappa light chains found on two thirds of mammalian antibodies.200111587642
[comparative study of chromatography-mass spectrography of microorganism's chemical markers in blood and intestinal mucosa bioptats].fatty acids, that are microorganism's markers in blood and jejunum's, ileum's, and large intestine's bioptats of the patients with irritable bowel syndrome were investigated with gas chromatography. markers of cl. perfringens, cl. difficile, enterococcus, streptomyces, enterobacterial, klebsiella, e. coli, peptostreptococcus, candida albicans, genus of streptococcus, of staphylococcus, of fusobacterium sp and others microorganisms were revealed. settling of intestinal mucosa was the same in jeju ...200111565126
bacteriology of adenoids and tonsils in children with recurrent adenotonsillitis.adenoids and tonsils electively removed from 25 children with a history of recurrent group a beta-hemolytic streptococci (gabhs) adenotonsillitis were cultured for aerobic and anaerobic bacteria. two hundred twenty-four organisms (112 aerobes and facultatives, 110 anaerobes, and 2 candida albicans) were isolated from the tonsils, and 229 (111 aerobes and facultatives and 118 anaerobes) were isolated from the adenoids. mixed infection was present in all instances, with an average of 9.1 isolates ...200111558761
[soft tissue infections caused by non-group a streptococci]. 200111555914
molecular identification of microorganisms from endodontic infections.a relatively wide range of bacteria have been isolated from root canals using standard culture techniques. however, only 50% of the bacteria in the oral cavity are cultivable (s. s. socransky et al., arch. oral biol. 8:278-280, 1963); hence, bacterial diversity in endodontic infections is underestimated. this study used a pcr-based 16s rrna gene assay, followed by cloning and sequencing of 16s rrna amplicons from a small subset of samples to assess the diversity of bacteria present in infected r ...200111526164
molecular analysis of peptostreptococcus micros isolates from patients with periodontitis.recent studies provide strong evidence implicating peptostreptococcus micros in the pathogenesis of various oral infections, including oropharyngeal abscesses and periodontal disease. to date, very little is known regarding the role of p. micros in periodontal disease. therefore, a genetic analysis was initiated to differentiate among strains of p. micros infecting periodontal patients.200111495135
viable bacteria in root dentinal tubules of teeth with apical periodontitis.two sets of teeth with apical periodontitis were collected at different geographic locations to study the identity of bacteria left in the root dentinal tubules. root dentin of 20 of these teeth was cultured from three locations between pulp and cementum (a, b, and c). in addition dentin from eight teeth was examined histologically. using the culturing technique bacteria were found in 77% of the dentin samples from set 1 (amsterdam) and in 87.5% of the dentin samples from set 2 (glasgow). at gre ...200111491642
proposal of the genera anaerococcus gen. nov., peptoniphilus gen. nov. and gallicola gen. nov. for members of the genus peptostreptococcus.members of genus peptostreptococcus have previously been found to be distantly related to the type species, peptostreptococcus anaerobius, on the basis of 16s rdna sequence similarities. they were divided into three major phylogenetic groups, and their peptidoglycan structure and biochemical traits differed between groups. the reclassification of the species of these three groups into three new genera, peptoniphilus gen. nov., anaerococcus gen. nov. and gallicola gen. nov., is proposed. the genu ...200111491354
the clinical relevance of microbiologic testing: a comparative analysis of microbiologic samples secured from the same sites and cultured in two independent laboratories.a field study using five different private periodontal practices was conducted; it compared two microbiologic culture samples simultaneously secured from the same sites within 23 individual patients and submitted for bacterial identification and antibiotic sensitivity testing to two separate laboratories. the results from the two laboratories were often different. in no instance did both laboratories agree on the presence of identical bacterial species. when only bacteria above threshold levels ...200111490400
antibacterial properties of electron beam-sterilized gutta-percha cones.the purpose of the study was to determine the effect of electron beam sterilization on gutta-percha cones (gpcs) at different times after sterilization. an agar diffusion test was used with -one aerobic bacterium (bacillus subtilis) and five oral anaerobic bacteria (fusobacterium nucleatum, peptostreptococcus micros, porphyromonas gingivalis, propionibacterium acnes, and veillonella parvula). with each microorganism 30 agar plates were prepared, evenly distributed among three groups (group 1: un ...200111487145
an in vitro evaluation of the antimicrobial efficacy of irrigants on biofilms of root canal isolates.the bactericidal effect of four antimicrobial agents was investigated against single-species biofilms derived from a range of root canal isolates.200111482142
effect of amoxycillin with or without clavulanate on adenoid bacterial flora.the effect of antimicrobial therapy with amoxycillin (amx) or co-amoxiclav (amc) on the adenoid bacterial flora of 45 children with recurrent otitis media (rom), scheduled for elective adenoidectomy, was studied. patients were randomized before surgery into three groups of 15, having had either no antibiotic therapy (control), or 10 days of therapy with amx or amc. core adenoid tissues were quantitatively cultured for aerobic and anaerobic bacteria. polymicrobial aerobic-anaerobic flora was pres ...200111481299
[bacteriologic investigation of periodontal infections].the gingivitis and periodontitis are very common diseases in hungary: more than 80% of the adult population suffers with gingivitis and 15-20% has destructive periodontitis. the composition of peridontopathogenic bacterial flora is totally different from that of the bacterial flora causing dental infections. in periodontal infections dominantly facultative and obligatory anaerobic microorganisms occur (actinobacillus actinomycetemcomitans (a. actinomycetemcomitans), porphyromonas gingivalis (p. ...200111480243
bacteriology and beta-lactamase activity in acute exacerbation of chronic assess the bacteriology of beta-lactamase (bl) enzyme activity in sputum of 40 patients with acute exacerbation of chronic bronchitis (aecb).200111468101
antibacterial activity and post-antibiotic effect of flurithromycin compared with other macrolides and penicillins against periodontal this study the authors examined the activity of flurithromycin compared to that of erythromycin, spiramycin and penicillin against 107 strains of various species supposed to cause periodontitis. the range of mics of flurithromycin was: < or =0.06-2 mg/l for p. gingivalis (28 isolates), 0.06-2 mg/l for p. melaninogenica (7), 0.5-4 mg/l for p. intermedia (5), 0.25-8 mg/l for prevotella sp. (8), 1-16 mg/l for f. nucleatum (14), 0.12-0.5 mg/l for w. recta (2), 0.5-32 mg/l for e. corrodens, 0.5-2 ...200111450882
chi1 torsion angle dynamics in proteins from dipolar couplings.experiments are presented for the measurement of one-bond carbon-proton dipolar coupling values at ch and ch2 ositions in 13c-labeled, approximately 50% fractionally deuterated proteins. 13cbeta-1hbeta dipolar couplings have been measured for 38 of 49 possible residues in the 63-amino-acid b1 domain of peptostreptococcal protein l in two aligning media and interpreted in the context of side-chain chi1 torsion angle dynamics. the beta protons for 18 of the 25 beta-methylene-containing amino acids ...200111448195
recovery of anaerobic bacteria from four children with postthoracotomy sternal wound infection.the cases of 4 children who developed postthoracotomy sternal wound infection caused by anaerobic bacteria are presented. the predominate anaerobes were peptostreptococcus species and pigmented prevotella species. polymicrobial infection was present in all cases, and aerobic bacteria also were recovered in 2 instances. all patients responded to surgical debridement and antimicrobials effective against the isolated aerobic and anaerobic bacteria. these findings highlight the potential importance ...200111433096
nugent score related to vaginal culture in pregnant relate gram-stained smears, using the nugent criteria, to quantitative and qualitative vaginal cultures in pregnant women.200111430961
cavernous sinus thrombophlebitis caused by sphenoid sinusitis--report of autopsy case.a 34-year-old man developed fever, headache, nausea, double vision, exophthalmus, ptosis, disturbance of vision and oculomotor nerve palsy. magnetic resonance imaging and cerebral angiography led to the clinical diagnoses of cavernous sinus thrombophlebitis and suspicion of bacterial aneurysm of the left internal carotid artery, respectively. peptostreptococcus was detected in blood culture analysis. he died 15 days after admission. systemic organs showed several septic changes. in particular, t ...200111430492
computer-based redesign of a protein folding pathway.a fundamental test of our current understanding of protein folding is to rationally redesign protein folding pathways. we use a computer-based design strategy to switch the folding pathway of protein g, which normally involves formation of the second, but not the first, beta-turn at the rate limiting step in folding. backbone conformations and amino acid sequences that maximize the interaction density in the first beta-hairpin were identified, and two variants containing 11 amino acid replacemen ...200111427890
mutans streptococci in subgingival plaque of treated and untreated patients with periodontitis.the etiology of root caries is thought to be identical to coronal caries, though root caries seem to be more complicated because of the higher susceptibility of exposed roots (dentin) by periodontal therapy to demineralization than intact enamel. this implies that mutans streptococci are the most likely pathogens in the development of root caries. although it is known that both the numbers of mutans streptococci and the frequency of isolation in root caries lesions are negatively correlated with ...200111422591
untreated periodontal disease in indonesian adolescents. subgingival microbiota in relation to experienced progression of an indonesian population deprived of regular dental care, the experienced progression of disease between baseline (1987) and follow-up (1994) was investigated in relation to the composition of the subgingival microbiota at follow-up. at baseline the age ranged from 15 to 25 years. clinical and microbiological evaluation was completed in 158 of the 167 subjects available at follow-up.200111422582
microbiological characteristics of subgingival microbiota in adult periodontitis, localized juvenile periodontitis and rapidly progressive periodontitis describe the prevalence of the cultivable subgingival microbiota in periodontal diseases and to draw attention to the polymicrobial nature of periodontic infections.200111422244
role of aromatic stacking interactions in the modulation of the two-electron reduction potentials of flavin and substrate/product in megasphaera elsdenii short-chain acyl-coenzyme a dehydrogenase.the effects of aromatic stacking interactions on the stabilization of reduced flavin adenine dinucleotide (fad) and substrate/product have been investigated in short-chain acyl-coenzyme a dehydrogenase (scad) from megasphaera elsdenii. mutations were made at the aromatic residues phe160 and tyr366, which flank either face of the noncovalently bound flavin cofactor. the electrochemical properties of the mutants were then measured in the presence and absence of a butyryl-coa/crotonyl-coa mixture. ...200111412126
[pharmacological and pharmacokinetic properties of azithromycin (zithromac), a novel 15-membered ring macrolide antibacterial agent].azithromycin (zithromac), a 15-membered ring macrolide antibacterial agent, was approved to be manufactured in japan in march 2000. it showed good in vitro and/or in vivo antibacterial activities against staphylococcus aureus, streptococcus pneumoniae, streptococcus pyogenes, peptostreptococcus micros, haemophilus influenzae, moraxella catarrhalis, mycoplasma pneumoniae and chlamydia pneumoniae. its activity against h. influenzae was particularly more potent than that of currently used macrolide ...200111411344
in vitro activity and post-antibiotic effect of quinupristin/dalfopristin (synercid).the in vitro activities of quinupristin/dalfopristin (synercid), ampicillin, erythromycin, clarithromycin, vancomycin, teicoplanin, ciprofloxacin and tetracycline were examined and compared against 526 gram-positive bacteria. the minimal inhibitory concentrations (mics) for quinupristin/dalfopristin against staphylococcus aureus, including methicillin-resistant strains, were low (mic(90) = 0.5 mg/l), and were comparable with those of vancomycin and teicoplanin. this compound was superior to the ...200111399860
smoking affects the subgingival microflora in periodontitis.tobacco smoking has been identified as one major risk factor for destructive periodontal disease. scaling and root planing have been shown to be less effective in smokers with periodontitis. the aim of the present study was to compare the subgingival microbial flora of treated and untreated smokers and non-smokers.200111394403
recovery of anaerobic bacteria from 3 patients with infection at a pierced body site.we describe 3 adolescents who developed infections due to anaerobes at pierced body sites: the nipple, the umbilicus, and the nasal septum. anaerobes (prevotella intermedia and peptostreptococcus anaerobius) were recovered from pure culture of specimens obtained from 1 patient with nipple infection and were mixed with aerobic bacteria in cultures of specimens obtained from 2 patients (streptococcus aureus, peptostreptococcus micros, and prevotella melaninogenica were recovered from a patient wit ...200111389513
vaginal microflora associated with bacterial vaginosis in nonpregnant women: reliability of sialidase determine the prevalence of gardnerella vaginalis, anaerobic bacteria and mycoplasma hominis in vaginal specimens of women with and without bacterial vaginosis (bv) as well as to determine the sensitivity and specificity of the direct sialidase assay of vaginal fluid as a rapid test for diagnosing this syndrome.200111368254
comparative in vitro activities of gemifloxacin, other quinolones, and nonquinolone antimicrobials against obligately anaerobic bacteria.the in vitro activity of gemifloxacin was compared to that of other quinolone and nonquinolone antimicrobials against 204 anaerobes by the agar dilution technique. the data indicate that gemifloxacin has a rather selective anaerobic activity. most peptostreptococcus, porphyromonas, and fusobacterium species are susceptible, while gemifloxacin's activity against other gram-negative anaerobes appears to be variable.200111353648
relationship of cigarette smoking to the subgingival microbiota.the relationship of cigarette smoking to the composition of the subgingival microbiota is not clear. some studies indicated higher levels of certain species in smokers, while other studies failed to detect differences in the microbiota between subjects with different smoking histories. thus, the purpose of the present investigation was to examine the prevalence, proportions and levels of the subgingival species in adult subjects who were current, past or never smokers.200111350499
bacteria isolated after unsuccessful endodontic treatment in a north american population.the purpose of this study was to determine the composition of the microbial flora present in teeth after the failure of root canal therapy in a north american population. these results were then compared with those of the previous scandinavian studies.200111346739
a microbiological study of papillon-lefévre syndrome in two analyse the microflora of subgingival plaque from patients with papillon-lefévre syndrome (pls), which is a very rare disease characterised by palmar-plantar hyperkeratosis with precocious periodontal destruction.200111328836
linezolid: a review of its use in the management of serious gram-positive infections.linezolid is the first of a new class of antibacterial drugs, the oxazolidinones. it has inhibitory activity against a broad range of gram-positive bacteria, including methicillin-resistant staphylococcus aureus (mrsa), glycopeptide-intermediate s. aureus (gisa), vancomycin-resistant enterococci (vre) and penicillin-resistant streptococcus pneumoniae. the drug also shows activity against certain anaerobes, including clostridium perfringens, c. difficile, peptostreptococcus spp. and bacteroidesfr ...200111324682
[cellulitis and necrotizing fasciitis: microbiology and pathogenesis].streptococcus pyogenes is a common cause of necrotizing cutaneous infections in otherwise healthy children and adults. several surface components are involved in the processes of adherence and invasiveness, such as protein m and capsulae. streptolysin o and other bacterial products, such as pyrogenic exotoxins, are involved in tissue injury and necrosis. toxins a and c act as superantigens and are expressed by strains associated with the toxic shock syndrome. staphylococcus aureus, alone or in a ...200111319369
amoxicillin plus metronidazole in the treatment of adult periodontitis patients. a double-blind placebo-controlled study.the aim of this double-blind, parallel study was to evaluate the adjunctive effects of systemically administered amoxicillin and metronidazole in a group of adult periodontitis patients who also received supra- and subgingival debridement.200111314884
immunoglobulin-binding domains of peptostreptococcal protein l enhance vaginal colonization of mice by streptococcus gordonii.protein l, an immunoglobulin-binding protein of some strains of the anaerobic bacterium peptostreptococcus magnus, has been hypothesized to be a virulence determinant in bacterial vaginosis. in order to investigate the role of protein l in peptostreptococcal virulence, the ig-binding domains of protein l were expressed at the surface of the human oral commensal streptococcus gordonii. recombinant streptococci were used in vaginal colonization experiments, and protein l-expressing s. gordonii dem ...200111312616
comparison of microbial composition in the subgingival plaque of adult crowded versus non-crowded dental has been reported in the literature that certain species of bacteria (periodontopathogens) present in the subgingival plaque are associated with destructive periodontal disease. the purpose of this study was to investigate and compare the presence and proportional distribution of periodontopathogens in the subgingival plaque of adult crowded versus non-crowded dental regions. thirty adult patients with anterior dental crowding were selected from the orthodontic clinic of the university of pen ...200011307190
the effect of statherin and its shortened analogues on anaerobic bacteria isolated from the oral cavity.the susceptibility (mic) of 44 strains of anaerobic bacteria isolated from the oral cavity and 3 standard strains to statherin and its c-terminal fragments with sequences qyqqytf, yqqytf, qqytf, qytf and ytf was determined by means of plate dilution technique in brucella agar with 5% content of defibrinated sheep's blood, menadione and hemin. the culture was anaerobic. as shown, at concentrations from 12.5 to 100 microg/ml statherin and its c-terminal fragments inhibited the growth of anaerobic ...200011293657
[the influence of anaerobic bacteria and mycoplasma isolated from the genital tract on mast cell activation].nowadays, it is known that mast cells, numerously appearing in all organs and being a source of a wide range of mediators and cytokines, are involved both in physiological and pathological processes. the aim of our study was to examine whether vaginal bacteria, especially those participating in bacterial vaginosis, are able to activate mast cells to mediators secretion. the study was done on rat peritoneal mast cells. the mast cells were incubated in vitro with suspensions of bacteroides capillo ...200011286181
smoking and subgingival microflora in periodontal disease.the present investigation was undertaken to analyze the influence of smoking on the periodontal disease associated subgingival microflora. the population included 33 smokers and 31 non-smokers in the age range 36-86 years.200111284533
intraabdominal abscess managed successfully via the laparoscopic approach.a rare complication of laparoscopic fundoplication-an intraabdominal abscess located between the fundus and the caudate lobe of the liver-is described. a 41-year-old man had undergone a laparoscopic nissen-rossetti fundoplication for longstanding gastroesophageal reflux disease. on the 5th postoperative day, the patient's general condition became worse, and he developed intermittent-remittent fever (40 degrees c), an elevated white blood cell count (wbc), and an accelerated sedimentation rate. e ...200011265065
structures of the b1 domain of protein l from peptostreptococcus magnus with a tyrosine to tryptophan substitution.the three-dimensional structure of a tryptophan-containing variant of the igg-binding b1 domain of protein l has been solved in two crystal forms to 1.7 and 1.8 a resolution. in one of the crystal forms, the entire n-terminal histidine-tag region was immobilized through the coordination of zinc ions and its structural conformation along with the zinc coordination scheme were determined. however, the ordering of the histidine tag by zinc does not affect the overall structure of the rest of the pr ...200111264576
[bacteriological study of chronic maxillary sinusitis in adults and observation of susceptibility to antibiotics].the unilateral mucosal samples were taken from 42 patients and were cultured for bacteria. meanwhile, the antimicrobial susceptibility was determined for 11 antibiotics and the beta-lactamase-producing bacteria(beta lpb) were examined. in 85.71% of all samples, bacterial growth was present. anaerobic bacteria were present in 21.43% of cases. predominant aerobic bacteria were alpha-hemolytic streptococci and coagulase-negative staphylococci. predominant anaerobic bacteria were peptostreptococcus ...199811263213
multicenter survey of the changing in vitro antimicrobial susceptibilities of clinical isolates of bacteroides fragilis group, prevotella, fusobacterium, porphyromonas, and peptostreptococcus vitro surveys of antimicrobial resistance among clinically important anaerobes are an important source of information that can be used for clinical decisions in the choice of empiric antimicrobial therapy. this study surveyed the susceptibilities of 556 clinical anaerobic isolates from four large medical centers using a broth microdilution method. piperacillin-tazobactam was the only antimicrobial agent to which all the isolates were susceptible. similarly, imipenem, meropenem, and metronidaz ...200111257040
microbial analysis of bone collected during implant surgery: a clinical and laboratory implant surgery produces bone debris which can be used to correct bone defects in the "simultaneous-augmentation" technique. however, this debris is potentially contaminated with oral bacteria. therefore, this study examined bone debris collected during dental implant surgery in order 1) to identify the microbial contaminants and 2) to compare the effects of two different aspiration protocols on the levels of microbial contamination. twenty-four partially dentate patients were randomly al ...200111251657
microorganisms isolated from root canals presenting necrotic pulp and their drug susceptibility in vitro.the knowledge about causative agents involved in endodontic infections is increasing, especially due to the improvement of culture techniques for anaerobic bacteria, showing that these microorganisms are predominant in this pathology. in this study, 31 canals with pulp necrosis were microbiologically analyzed before and after manipulation. obligate and facultative anaerobes, microaerophilic bacteria and yeasts were recovered from 24, 14, 5 and 2 clinical specimens, respectively. the most frequen ...200111240863
detection of peptostreptococcus micros dna in clinical samples by pcr.peptostreptococcus micros is a gram-positive anaerobic coccus which, although considered to be a natural commensal of the human oral cavity, is associated with periodontal, endodontal and peritonsillar infections. identification of the organism has to date relied upon conventional culture methods and biochemical analyses. the purpose of this study was to develop a pcr method for rapid and specific identification of this organism in clinical samples. a pair of primers was selected, each of which ...200111232771
correlation between microbiology and previous sinus surgery in patients with chronic maxillary sinusitis.aspirates of 108 chronically inflamed maxillary sinuses were processed for aerobic and anaerobic bacteria. there were 295 bacterial isolates: 109 aerobic and facultative, and 186 anaerobic. the predominant aerobic isolates were staphylococcus aureus (17 isolates), alpha-hemolytic streptococci (14), pseudomonas aeruginosa (12), moraxella catarrhalis (10), and haemophilus spp (8). the predominant anaerobes were peptostreptococcus spp (61), prevotella spp (45), fusobacterium spp (15), and propionib ...200111219522
antagonism against vibrio cholerae by diffusible substances produced by bacterial components of the human faecal microbiota.cholera vibrios sometimes survive, probably in low-level silent populations, in the small intestine of chronic carriers or pass through the gastrointestinal tract of a few individuals without causing diarrhoea or colonisation. to understand these situations, the present study used plate cultures (ex-vivo test) to investigate the frequency of appearance of an inhibitory halo against vibrio cholerae produced by faecal specimens from 92 healthy volunteers (40 females, 52 males) aged 4-61 years. the ...200111211223
molecular epidemiology and association of putative pathogens in root canal infection.the purpose of this study was to investigate the epidemiology of putative pathogens in root canals with apical periodontitis and to determine the associations among the putative pathogens. eighteen symptomatic and 20 asymptomatic teeth from 36 subjects were studied. this research was performed with polymerase chain reaction and hybridization using rrna-based oligonucleotide probes. the most frequently found species was fusobacterium sp. (68.4%), followed by peptostreptococcus micros (44.7%) and ...200011199802
growth inhibitory activity of gutta-percha points containing root canal medications on common endodontic bacterial pathogens as determined by an optimized quantitative in vitro assay.gutta-percha points containing calcium hydroxide, zinc oxide (zno), a mixture of zno and chlorhexidine (zno/chx), iodine-polyvinylpyrrolidone (zno/j-pvp), or a mixture of chx and j-pvp and zno (zno/chx/j-pvp) were tested for their ability to inhibit growth of pure cultures of bacterial species commonly involved in endodontic infections (peptostreptococcus micros, streptococcus intermedius, enterococcus faecalis, and porphyromonas gingivalis). to quantitate growth inhibition, an in vitro assay wa ...200011199764
statistical theory for protein combinatorial libraries. packing interactions, backbone flexibility, and the sequence variability of a main-chain structure.combinatorial experiments provide new ways to probe the determinants of protein folding and to identify novel folding amino acid sequences. these types of experiments, however, are complicated both by enormous conformational complexity and by large numbers of possible sequences. therefore, a quantitative computational theory would be helpful in designing and interpreting these types of experiment. here, we present and apply a statistically based, computational approach for identifying the proper ...200111178917
early colonization of dental implants by putative periodontal pathogens in partially edentulous patients.there is limited scientific information available on the early colonization of the peri-implant pockets in partially edentulous individuals. knowledge about this process is one step in better understanding the etiology and pathogenesis of peri-implantitis. in this study, the early colonization of the peri-implant pockets by putative periodontal pathogens was studied in 20 partially edentulous individuals using anaerobic culture techniques. at baseline, the presence and levels of putative periodo ...200011168244
calibration of the disk diffusion test for trovafloxacin susceptibility testing of four anaerobic study trovafloxacin susceptibility among clinical isolates of four anaerobic bacterial species using minimum inhibitory concentrations (mic) determinations, e test assays and disk diffusion test results and to calibrate the disk diffusion method for these species using single strain regression analysis (sra).200011168107
interleukin-6 deficiency increases inflammatory bone destruction.periapical bone destruction occurs as a consequence of pulpal infection. in previous studies, we showed that interleukin-1 (il-1) is the primary stimulator of bone destruction in this model. il-6 is a pleiotropic cytokine that is induced in these infections and has both pro- and anti-inflammatory activities. in the present study, we determined the role of il-6 in regulating il-1 expression and bone resorption. the first molars of il-6 knockouts (il-6(-/-)) and wild-type mice were subjected to su ...200111159963
macrolide resistance in peptostreptococcus spp. mediated by ermtr: possible source of macrolide-lincosamide-streptogramin b resistance in streptococcus pyogenes.eighty percent (21 of 26) of macrolide-resistant peptostreptococcus strains studied harbored the ermtr gene. this methyltransferase gene is also the most frequently found gene among macrolide-lincosamide-streptogramin b-resistant streptococcus pyogenes strains. transfer of the ermtr gene from peptostreptococcus magnus to macrolide-susceptible s. pyogenes strains indicates that this resistance determinant may circulate among gram-positive aerobic and anaerobic species of the oropharyngeal bacteri ...200111158770
two cases of diskitis attributable to anaerobic bacteria in children.diskitis, an inflammation of the intervertebral disk, is generally attributable to staphylococcus aureus and rarely staphylococcus epidermidis, kingella kingae, enterobacteriaciae, and streptococcus pneumoniae. in many cases, no bacterial growth is obtained from infected intervertebral discs. although anaerobic bacteria were recovered from adults with spondylodiscitis, these organisms were not reported before from children. the recovery of anaerobic bacteria in 2 children with diskitis is report ...200111158500
profile of subgingival microbiota in children with mixed dentition.a diversity of microbial species has been detected in children's oral flora at an early age. to investigate the composition of the subgingival microbiota of different groups of teeth in children with mixed dentition, 40 systemically healthy children, aged 7-8 years, randomly chosen, were examined. subgingival plaque samples were taken from the mesiobuccal sites of 21, 41, 16 and 36 permanent teeth and 53, 73, 64 and 84 deciduous teeth. the samples were cultured for bacterial isolation anaerobica ...200011155173
studies on a single immunoglobulin-binding domain of protein l from peptostreptococcus magnus: the role of tyrosine-53 in the reaction with human igg.chemical modification experiments with tetranitromethane (tnm) have been used to investigate the role of tyrosine residues in the formation of the complex between ppl (the single ig-binding domain of protein l, isolated from p. magnus strain 3316) and the kappa light chain (kappa-chain). reaction of ppl with tnm causes the modification of 1.9 equiv. of tyrosine (tyr(51) and tyr(53)) and results in an approx. 140-fold decrease in affinity for human igg. similar experiments with mutated ppl protei ...200111139405
peptostreptococcus asaccharolyticus renal abscess: a rare cause of fever of unknown origin.renal abscess is uncommon in pediatrics and is rarely a cause of fever of unknown origin. we recently cared for a patient who presented with a 3-week history of fever. an indium scan ultimately led to the diagnosis of a renal abscess. aspiration yielded peptostreptococcus asaccharolyticus. this unusual case prompted a review of the clinical and microbiologic features of renal abscess in pediatric patients at our hospital over the past 10 years. seven additional patients with a discharge diagnosi ...200111134475
comparative in vitro activities of abt-773 against 362 clinical isolates of anaerobic bacteria.the activity of abt-773, a novel ketolide antibiotic, against clinical isolates of anaerobic bacteria was determined and compared to the activities of other antimicrobial agents. mics at which 90% of isolates were inhibited (mic(90)s) were </=0.06 microg/ml for actinomyces spp., clostridium perfringens, peptostreptococcus spp., propionibacterium spp., and porphyromonas spp. the mic(50)s and mic(90)s were </=0.06 and >32 microg/ml, respectively, for eubacterium spp., lactobacillus spp., clostridi ...200111120995
transgenic rainbow trout expressed sgnrh-antisense rna under the control of sgnrh promoter of atlantic salmon.a recombinant vector containing antisense dna complementary to atlantic salmon (salmo salar) sgnrh cdna driven by specific promoter pab derived from a corresponding sgnrh gene was introduced into rainbow trout (oncorhynchus mykiss) eggs. this resulted in transgenic animals that had integrated one copy of the transgene into their genome and transmitted it through the germline. antisense-sgnrh mrna (as) was expressed mainly in the brain of transgenic as(+) fish. levels of sgnrh endogenous mrna in ...200011116212
apparent culture-negative prosthetic valve endocarditis caused by peptostreptococcus two patients with prosthetic valve endocarditis due to peptostreptococcus magnus, blood cultures in the bact/alert and bactec 9240 systems were signal negative. the capability of the bact/alert system to detect various peptostreptococcus species was assessed. p. magnus and p. anaerobius could not be detected, and subcultures remained negative. the growth in conventional media of these two species and other peptostreptococcus species was similar.200011101611
bacteriology of histopathologically defined appendicitis in children.acute appendicitis is the most common surgical emergency in childhood. however, the pathogenesis and detailed microbiology are obscure.200011099090
human intestinal bacteria capable of transforming secoisolariciresinol diglucoside to mammalian lignans, enterodiol and metabolites were isolated after anaerobic incubation of secoisolariciresinol diglucoside (1) with a human fecal suspension. they were identified as (-)-secoisolariciresinol (2), 3-demethyl-(-)-secoisolariciresinol (3), 2-(3-hydroxybenzyl)-3-(4-hydroxy-3-methoxybenzyl)butane-1,4-diol (4), didemethylsecoisolariciresinol (5), 2(3-hydroxybenzyl)-3-(3,4-dihydroxybenzyl)butane-1,4-diol (6), enterodiol (7) and enterolactone (8). furthermore, two bacterial strains, peptostreptococcus sp. sdg-1 and ...200011086885
the effects of amoxicillin therapy on skin flora in order to determine the effect of amoxicillin therapy on the perineal skin microbial flora in infants, we took quantitative bacterial and fungal cultures of perineal and sternal areas from 25 infants treated with amoxicillin (40 mg/kg/day) for 10 days. specimens were obtained prior to therapy, within 3 days of conclusion of therapy, and 14-16 days later. immediately following therapy, a decline in the number of bacterial isolates occurred on both the perineum (89 to 47) and sternum (84 to 39). ...200011085662
bacteriology in disease: failure of a micro-ecological balance?both aerobic (mainly alpha-haemolytic streptococci) and anaerobic micro-organisms (like prevotella and peptostreptococcus species) are able to interfere with the growth of potential pathogens such as group a beta-haemolytic streptococci, streptococcus pneumoniae, haemophilus influenzae and moraxella catarrhalis. the present paper reviews the application of this concept of interference with respect to the development of common acute ear-, nose- and throat infections.200011082778
[successful treatment for descending necrotizing mediastinitis: a case report].a 21-year-old female was admitted to our hospital because of high fever, neck swelling, and dyspnea. she was diagnosed as descending necrotizing mediastinitis (dnm) extended from odontogenic infection. on the day of admission, she underwent cervical drainage. next day, the ct scan showed an abscess below the tracheal bifurcation and bilateral pleural effusion. mediastinal drainage was performed through a right thoracotomy, and a left thoracic tube was inserted. anaerobic peptostreptococcus was f ...200011079317
bacterial susceptibility to amoxicillin and potassium clavulanate in advanced periodontitis patients not responding to mechanical therapy.between 4 and 8% of periodontitis patients are reported to respond poorly to conventional therapy. in these cases, adjunctive use of systemic antibiotics might be a reasonable therapeutic approach. the purpose of this study was to evaluate the effects of systemic amoxicillin/clavulanate as adjunct to periodontal surgery on the predominant subgingival microorganisms in patients not responding to mechanical therapy. furthermore, the bacterial susceptibility to amoxicillin/clavulanate was analyzed ...200011073328
microbiological parameters associated with il-1 gene polymorphisms in periodontitis patients.polymorphisms in the cluster of il-1 genes have been significantly associated with the severity of adult periodontitis. the purpose of this study was to compare microbiological parameters in il-1 genotype negative and positive adult subjects with a range of periodontitis severities.200011073323
[bacteria isolated from surgical infections and their susceptibilities to antimicrobial agents. special references to bacteria isolated between april 1997 and march 1998].the annual multicenter studies on isolated bacteria from infections in general surgery and their antimicrobial susceptibility have been conducted in 19 facilities in japan since july 1982. this paper describes the results obtained during the period from april 1997 to march 1998. the number of cases investigated as objectives was 215 for one year. a total of 420 strains (170 strains from primary infections and 250 strains from postoperative infections) were isolated from 174 cases (80.9% of total ...200011070817
differences in the composition of the subgingival microbiota of two periodontitis populations of different geographical origin. a comparison between spain and the netherlands.the purpose of this study was to compare the subgingival microbiota of two geographically distinct patient populations using identical clinical and bacteriological methods. adult patients with a diagnosis of periodontitis were consecutively selected according to pre-defined clinical criteria. microbiological samples were taken from the deepest four sites with bleeding. the samples were plated on blood agar plates, for the determination of the total anaerobic counts and identification of specific ...200011037754
detection of major putative periodontopathogens in korean advanced adult periodontitis patients using a nucleic acid-based approach.although extensive microbial analyses have been performed from subgingival plaque samples of periodontitis patients, systematic analysis of subgingival microbiota has not been carried out in a korean population so far. the purpose of this study was to describe the prevalence of major putative periodontopathogens in korean patients by culture-independent methods.200011022767
the microbial spectrum of brain abscess with special reference to anaerobic bacteria.the bacteriological and clinical findings of 18 cases of brain abscess are presented. identification and antimicrobial susceptibility of anaerobic strains was performed both by conventional methods and the newer rapid ana ii panel and e-test methods, respectively. characterization of the anaerobic isolates was done by restriction fragment length polymorphism (rflp). anaerobic or aerobic organisms alone were recovered in three (16.6%), and mixed aerobic and anaerobic in another three (16.6%) pati ...199811013664
[tracheopulmonary-subcutaneous fistula associated with anaerobic subcutaneous abscess on mechanical ventilator support in a patient with duchenne muscular dystrophy].a 29-year-old japanese man with duchenne muscular dystrophy was placed on a mechanical ventilator support at 23 years of age and admitted to our hospital at 25 years of age. he had severe neck contracture deviated to the left side which resulted in dysphagia and microaspiration. at 29 years of age, he developed left lobar pneumonia accompanied by slight fever, back pain and a foul odor from the patient's sputum. although the patient received broad spectrum antibiotics, pneumonia disseminated to ...200011002735
b-cell deficiency predisposes mice to disseminating anaerobic infections: protection by passive antibody transfer.we have previously demonstrated that a high proportion of rag-2 scid knockout mice, which lack t and b cells, develop orofacial abscesses and disseminated infections following pulpal infection, whereas immunocompetent control mice do not. in the present study, we sought to identify the components of the adaptive immune response which contribute to protection against disseminating anaerobic infections and sepsis. for this purpose, various genetically engineered immunodeficient mice were employed, ...200010992465
bacterial immunoglobulin superantigen proteins a and l activate human heart mast cells by interacting with immunoglobulin e.human heart mast cells (hhmc) have been identified in heart tissue, perivascularly, and in the intima of coronary arteries. in vitro activation of isolated hhmc induces the release of vasoactive and proinflammatory mediators (histamine, tryptase, and cysteinyl leukotriene c(4) [ltc(4)]). we investigated the effects of several bacterial proteins on hhmc activation in vitro. hhmc released histamine, tryptase, and ltc(4) in response to staphylococcus aureus cowan 1 and the immunoglobulin (ig)-bindi ...200010992448
comparative in vitro activities of gar-936 against aerobic and anaerobic animal and human bite wound pathogens.gar-936 is a new semisynthetic glycylcycline with a broad antibacterial spectrum, including tetracycline-resistant strains. the in vitro activities of gar-936, minocycline, doxycycline, tetracycline, moxifloxacin, penicillin g, and erythromycin were determined by agar dilution methods against 268 aerobic and 148 anaerobic strains of bacteria (including pasteurella, eikenella, moraxella, bergeyella, neisseria, ef-4, bacteroides, prevotella, porphyromonas, fusobacterium, staphylococcus, streptococ ...200010991855
laboratory diagnosis of anaerobic pleuropulmonary infections.anaerobic organisms play a major role in pleuropulmonary infections. clinical manifestations range from simple aspiration to acute, severe, necrotizing pneumonias to chronic infections, such as lung abscess and empyema. implicated pathogens are usually of endogenous origin. laboratory diagnosis of anaerobic pleuropulmonary infections is based on recovering the etiological agent from clinical specimens. appropriate specimens include pleuml fluid, transtracheal aspirates, transthoracic aspirates a ...200010983929
characterization of peptostreptococcus species in skin infections.we examined the characteristics of peptostreptococcus species in infectious skin diseases. p. magnus was the species identified most frequently, followed by p. asaccharolyticus. peptostreptococcus species were mainly isolated from infected atheroma and secondary infections due to ulcers; their resistance to five antimicrobial agents was generally low. the resistance of the three predominant peptostreptococcus species to the antimicrobials was similar to that of all of the peptostreptococcus spec ...200010983864
in vitro and in vivo antibacterial activities of a new injectable carbapenem, s-4661, against gynaecological pathogens.carbapenems are often used to treat infections in obstetrics and gynaecology because of their activity against anaerobes. s-4661, a new injectable carbapenem antibiotic, has favourable pharmacokinetic properties and is not hydrolysed by dehydropeptidase i. we investigated the in vitro and in vivo antibacterial activities of s-4661 against strains of streptococcus agalactiae, escherichia coli, peptostreptococcus magnus, bacteroides fragilis and prevotella bivia, which are major pathogens in the f ...200010980177
in vitro activity of an evernimicin derivative, sch27899, against anaerobic bacteria and propionibacterium acnes.the in vitro activity of sch27899, a novel oligosaccharide antimicrobial agent, was compared with those of representatives of six classes of antimicrobial agents (piperacillin, clarithromycin, clindamycin, vancomycin, sitafloxacin and metronidazole) against clinical isolates of anaerobic bacteria and propionibacterium acnes. against peptostreptococcus: spp. and clostridium difficile, sch27899 was the most potent (mic(90) < 0.125 mg/l) of the agents examined. besides these gram-positive anaerobes ...200010980176
post-caesarean septicaemia in kandang kerbau hospital, singapore, 1993-1995.we reviewed all documented cases of septicaemia following caesarean deliveries in kandang kerbau hospital between 1st january 1993 to 31st december 1995. there were 22 cases of septicaemia among 8201 caesarean births, and hence the incidence is 2.7:1000. there were 45,412 deliveries, and the overall caesarean section rate was 18.1%. among the 22 documented cases of septicaemia which came under this study, the most common clinical conditions found were endomyometritis (7 cases), urinary tract inf ...199710968108
aerobic and anaerobic microbiology in intra-abdominal infections associated with diverticulitis.the aerobic and anaerobic microbiology of intra-abdominal infections associated with diverticulitis was studied in 110 specimens from the peritoneal cavity after intestinal perforation and in 22 specimens from abdominal abscesses. anaerobic bacteria only were isolated from 17 (15%) of the peritoneal specimens, aerobic bacteria only from 12 (11%) and mixed aerobic and anaerobic flora from 81 (74%). a total of 339 bacterial isolates was detected in peritoneal cultures (3.1 per specimen), comprisin ...200010966232
comparative in vitro activities of abt-773 against aerobic and anaerobic pathogens isolated from skin and soft-tissue animal and human bite wound infections.we studied the comparative in vitro activities of abt-773, a new ketolide, against 268 aerobic and 148 anaerobic recent isolates from clinical bites using an agar dilution method and inocula of 10(4) cfu/spot for aerobes and 10(5) cfu for anaerobes. the following are the mic ranges and mics at which 90% of isolates are inhibited (mic(90)s) of abt-773 for various isolates, respectively: pasteurella multocida and pasteurella septica, 0.125 to 2 and 1 microg/ml; other pasteurella species, 0.125 to ...200010952607
[the treatment of bacterial vaginosis with dalacin v cream].to asses the efficiency of the 2% clindamycin phosphate creme given in 3 and 7 daily courses officially designer for the treatment of bacterial vaginosis (bv).200010948619
response of the central metabolism in corynebacterium glutamicum to the use of an nadh-dependent glutamate dehydrogenase.the extensive use of 13c enrichments in precursor metabolites for flux quantification does not rely on nadph stoichiometries and can therefore be used to quantify reducing power fluxes. as an application of this concept, the nadph fluxes were quantified in an l-lysine producer of corynebacterium glutamicum grown into metabolic and isotopic steady state with [1-13c]glucose. in this case, where the organism's nadph-dependent glutamate dehydrogenase consumes reducing power, the nadph flux generated ...199910935753
evaluation of a phenotypic scheme for identification of the 'butyrate-producing' peptostreptococcus species.gram-positive anaerobic cocci (gpac) are isolated from approximately one quarter of all infections involving anaerobic bacteria. however, studies of the significance of this group of pathogens have been hindered by an inadequate taxonomy and the lack of a valid identification scheme. in the present study, a phenotypic scheme for the identification of 'butyrate-producing' gpac based on the analysis of volatile fatty acid profiles by gas-liquid chromatography, biochemical profiles (including the u ...200010933261
bacterial vaginosis a "broad overview".bacterial vaginosis (bv) was first reported in 1995 by gardner and dukes, who described the unique clinical signs and symptoms and the distinctive nature of the vaginal discharge associated with it. they also described a "new" causative organism, which they named "haemophilus vaginalis", subsequently renamed gardnerella vaginalis. bv is currently the most prevalent cause of infectious vaginitis among women attending for genitourinary diseases. bv has a complex microbiology. lactobacillus populat ...199910932748
critical role of beta-hairpin formation in protein g folding.comparison of the folding mechanisms of proteins with similar structures but very different sequences can provide fundamental insights into the determinants of protein folding mechanisms. despite very little sequence similarity, the approximately 60 residue igg binding domains of protein g and protein l both consist of a single helix packed against a four-stranded sheet formed by two symmetrically disposed beta-hairpins. we demonstrate that, as in the case of protein l, one of the two beta-turns ...200010932252
in vitro bacterial interference in the nasopharynx of otitis media-prone and non-otitis media-prone compare the frequency of recovery of potential pathogens and aerobic- and anaerobic-interfering bacteria in the nasopharynx of otitis media-prone (omp) with that in non-omp (n-omp) children.200010922236
[bacterial vaginosis. epidemiology and risk factors].bacterial vaginosis (bv) is a change in vaginal ecosystem where lactobacilli dominate, flora is absent or greatly reduced, and replaced with a mixed, predominantly anaerobic flora, consisting of gardnerella vaginalis, mycoplasma hominis, mobiluncus spp, bacteroides spp, prevotela spp, peptostreptococcus spp, fusobacterium spp and porphyromonas spp. the concentration of bacteria increases from 100 to 1000 fold in women with bv compared to healthy women. bv has been formerly known as nonspecific v ...200010916461
"checkerboard" assessments of periodontal microbiota and serum antibody responses: a case-control study.we explored the association between subgingival microbial profiles and serum igg responses to periodontal microbiota in relation to clinical periodontal status.200010914791
characterization of bacterial orofacial infections using a new murine model.we devised a new murine orofacial infection model using bacteria from odontogenic infection origins and characterized the experimental infections. in this model, bacteria were injected into the submandible of mice. streptococcus constellatus and peptostreptococcus micros produced a single abscess at the injection site and their abscess-forming and lethal abilities were low: the median abscess-forming dose (af(50)) of s. constellatus and p. micros were 10(8.5-10.7)and 10(10.2-10.6)cfu/mouse, and ...200010906266
interleukin-1 receptor signaling rather than that of tumor necrosis factor is critical in protecting the host from the severe consequences of a polymicrobe anaerobic infection.infection of the dental pulp leads to an osteolytic lesion that results from a polymicrobial infection consisting largely of pathogenic anaerobes. infection causes significant morbidity and mortality mediated by bacterial factors and in some cases by the up-regulation of inflammatory cytokines. the inflammatory cytokines interleukin-1 (il-1) and tumor necrosis factor (tnf), in particular, play a complex and central role in the responses to microbial pathogens. however, relatively little is known ...200010899881
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