Publications

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fermentation of lactic acid by the rumen microorganism, peptostreptococcus elsdenii. 19655216438
studies of the predominant cultivable micro-organisms from the human tongue. 19665225869
[case of peptostreptococcal subacute bacterial endocarditis with pda]. 19695307945
[species and antibiotic sensitivity of anaerobes isolated from various clinical materials in the last 8 years]. 19705311041
studies on flavin binding in flavodoxins. 19715317635
evidence for a novel flavin prosthetic group associated with nadh dehydrogenase from peptostreptococcus elsdenii. 19715317636
acute and recurrent thromboembolic disease: a new concept of etiology. 19695344031
oxygen sensitivity of various anaerobic bacteria.anaerobes differ in their sensitivity to oxygen, as two patterns were recognizable in the organisms included in this study. strict anaerobes were species incapable of agar surface growth at po(2) levels greater than 0.5%. species that were found to be strict anaerobes were treponema macrodentium, treponema denticola, treponema oralis n. sp., clostridium haemolyticum, selenomonas ruminatium, butyrivibrio fibrisolvens, succinivibrio dextrinosolvens, and lachnospira multiparus. moderate anaerobes w ...19695370458
[subacute bacterial endocarditis causes by anaerobic peptostreptococcus with brief review of general clinical picture]. 19695377254
isolation and identification of rumen bacteria capable of anaerobic rutin degradation. 19695392469
the tryptophans in flavodoxin and synthetic flavinyl peptides characterized by chemical and photochemical oxidations. 19705417473
diagnosis and treatment of bacteroides and peptostreptococcal infections. 19705429732
[subacute bacterial endocarditis due to peptostreptococcus]. 19705458522
peptostreptococcus elsdenii from the caecum of pigs. 19705516605
chemical and physical characterization of the shethna flavoprotein and apoprotein and kinetics and thermodynamics of flavin analog binding to the apoprotein. 19715538602
carbon dioxide requirement of various species of rumen bacteria.the carbon dioxide requirement of 32 strains of rumen bacteria, representing 11 different species, was studied in detail. increasing concentrations of co(2) were added as nahco(3) to a specially prepared co(2)-free medium which was tubed and inoculated under nitrogen. prior depletion of co(2) in the inoculum was found to affect the level of requirement; however, the complexity and buffering capacity of the medium did not appear to be involved. an absolute requirement for co(2) was observed for e ...19715541030
variations in the amount and relative distribution of vitamin b12 and its analogs in the bovine rumen. 19715546184
nondenaturing procedure for rapid preparation of ferredoxin from clostridium pasteurianum. 19715556406
[studies on the occurrence and the frequency of various microorganisms (anaerobic bacteria, trichomonads, mycoplasmas and yeasts) in the human vagina in vaginitis]. 19675629109
non-heme iron proteins. v. the amino acid sequence of rubredoxin from peptostreptococcus elsdenii. 19685640967
[pathogenic significance of anaerobes especially non-sporulating anaerobes, in infection]. 19685672990
enzymic studies on the biosynthesis of amino acids from lactate by peptostreptococcus elsdenii.cell-free extracts of peptostreptococcus elsdenii, a strict anaerobe from the rumen, were examined for enzymes catalysing the steps in the biosynthesis from lactate of alanine, serine, aspartate and glutamate. extracts contain the enzymes necessary for the formation of alanine from lactate via pyruvate. the presence of enzymes catalysing the interconversion of phosphoglycerate and phosphohydroxypyruvate, the transamination of the latter to phosphoserine and the cleavage of phosphoserine to serin ...19685690537
[anaerobic bacteria isolated from the sputum and nasopharyngeal fluids]. 19685717385
pectin-fermenting bacteria isolated from the bovine rumen.thirty-two strains of pectin-fermenting rumen bacteria were isolated from bovine rumen contents in a rumen fluid medium which contained pectin as the only added energy source. based on differences in morphology and the gram stain, 10 of these strains were selected for characterization. two strains were identified as lachnospira multiparus, four strains were identified as butyrivbrio fibrisolvens, and three strains were identified as bacteroides ruminicola. characteristics of the remaining strain ...19695802604
isoleucine biosynthesis from 2-methylbutyric acid by anaerobic bacteria from the rumen.microorganisms in ruminal ingesta and pure cultures of anaerobic ruminal bacteria of different physiological and morphological groups incorporated (14)c from labeled 2-methylbutyrate during growth. the radioactivity was incorporated mainly into lipid and protein. isoleucine was the only labeled amino acid found in acid hydrolysates of protein from either pure or mixed cultures. radioactivity in isoleucine synthesized from 2-methylbutyrate-1-(14)c was entirely in carbon-2. thus, the carboxylation ...19695813342
chemical composition and serological analysis of the cell wall of peptostreptococcus.bahn, arthur n. (northwestern university, chicago, ill.), patrick c. y. kung, and james a. hayashi. chemical composition and serological analysis of the cell wall of peptostreptococcus. j. bacteriol. 91:1672-1676. 1966.-chemical and serological analyses were made of the cell wall of peptostreptococcus to characterize taxonomically this genus of anaerobic streptococci. cell wall hydrolysates of p. putridus strains 06 and 85, p. intermedius strains 11 and 87, and p. elsdenii strain b-159 were prep ...19665937229
importance of the isovalerate carboxylation pathway of leucine biosynthesis in the rumen.certain anaerobic ruminal bacteria synthesize the leucine carbon skeleton by use of a pathway different from that described in other microorganisms. these organisms carboxylate the intact carbon skeleton of isovalerate, synthesizing leucine-2-c(14) from isovalerate-1-c(14). strains of bacteroides ruminicola and peptostreptococcus elsdenii were like ruminococcus flavefaciens in that they incorporated appreciable amounts of c(14) from isovalerate-1-c(14) into cellular protein and in that the only ...19665970468
[adansonian taxonomy and the relationship of microorganisms in terms of the concepts of similarity value and center species. (2). on the tribe streptococcae of the family coccaceae]. 19666005767
tracer studies on the biosynthesis of amino acids from lactate by peptostreptococcus elsdenii.peptostreptococcus elsdenii, a strict anaerobe from the rumen, was grown on a medium containing yeast extract and [1-(14)c]- or [2-(14)c]-lactate. radioisotope from lactate was found in all cell fractions, but mainly in the protein. the label in the protein fraction was largely confined to a few amino acids: alanine, serine, aspartic acid, glutamic acid and diaminopimelic acid. the alanine, serine, aspartic acid and glutamic acid were separated, purified and degraded to establish the distributio ...19676069834
[in vitro activity and clinical evaluation of ceftriaxone in the field of obstetrics and gynecology].in vitro activity of ceftriaxone (ctrx) was examined by agar plates dilution method against 398 strains isolated from the infections in the field of obstetrics and gynecology. mic90 of ctrx against staphylococcus (107 strains), e. coli (54 strains), k. pneumoniae (27 strains), peptococcus and peptostreptococcus (106 strains) and bacteroides (104 strains) was more than 100 micrograms/ml, less than 0.20 micrograms/ml, less than 0.20 micrograms/ml, 6.25 micrograms/ml and 50 micrograms/ml, respectiv ...19846098728
antimicrobial susceptibility of anaerobic bacteria in sweden in 1983.this review is concerned with anaerobic bacteria and antimicrobial susceptibility. data on the susceptibility to different antimicrobials of anaerobic isolates in sweden during 1983 is presented. among the different bacterial groups, clostridium perfringens, propionibacteria, anaerobic cocci and curved rods were found to be susceptible to beta-lactam antibiotics, clindamycin and chloramphenicol. c. difficile was susceptible to benzylpenicillin and imipenem, but resistant to cefoxitin. most c. di ...19846100139
do anaerobes cause urinary tract infection? 19806101823
neutrophil chemotaxis induced by anaerobic bacteria isolated from necrotic dental pulps.anaerobic bacteria belonging to the species bacteroides melaninogenicus, bacteroides asaccharolyticus, fusobacterium nucleatum, peptostreptococcus anaerobius and eubacterium alactolyticum, isolated from necrotic dental pulps, were tested for their capacity to induce neutrophil leukocyte chemotaxis. generation of chemotactic factors in serum by whole bacterial cells and chemotaxis induced by bacterial extracts were studied in vitro by the boyden technique. subcutaneously implanted wound chambers ...19806103573
in vitro inhibition of neisseria gonorrhoeae growth by strict anaerobes.thirty two anaerobic bacterial strains were screened for their in vitro inhibitory activity toward 21 neisseria gonorrhoeae strains, including two penicillin-resistant strains. from this screening, six anaerobic bacteria belonging to the genera bacteroides, peptostreptococcus, and eubacterium were selected for their large antigonococcal spectrum of activity. the inhibitory activity of these six strains appears to be specific to the gonococcus as a variety of anaerobes and aerobes were not genera ...19806105130
acute suppurative parotitis associated with anaerobic bacteremia.most episodes of acute suppurative parotitis are caused by staphylococcus aureus. reported here is a patient who had acute parotitis associated with anaerobic sepsis caused by bacteroides melaninogenicus and peptostreptococcus micros. acute parotitis should be included in the ever-widening spectrum of clinical infections caused by anaerobic bacteria.19816110333
lactobacilli do not cause frequency and dysuria syndrome.mid-stream specimens (msu) of urine were collected from 142 healthy women (pregnant and non-pregnant) and cultured for lactobacilli and other fastidious bacteria. the latter either require co2 or are obligate anaerobes. lactobacilli were present in counts of 10(4)/ml or more in 34.8% of the women, and in counts of 10(5)/ml or more in 20.2%. besides lactobacilli, which were the bacteria most frequently isolated, anaerobic gram-positive cocci (peptococci and peptostreptococci) were often found. th ...19816115160
aerobic and anaerobic bacteriology of peritonsillar abscess in children.aspiration of peritonsillar abscess (quinsy) was aseptically performed in 16 children. patients' median age was 10 years (range 6 to 17 years), and 12 were males. unilateral abscess was present in all but one child. all aspirates were cultured for aerobes and anaerobes and yielded bacterial growth in all patients. anaerobes were isolated in all patients; in 3 patients (19%), they were the only organism isolated, and in 13 (81%), they were mixed with aerobes. there were 91 anaerobic isolates (5.7 ...19816119870
[anaerobic microflora of praepubertal children (author's transl)].bacteriologic cultures of the vagina and the cervix of 38 praepubertal children, aged 3 months to 16 years (average age 9 years) from the outpatient department for infants and juveniles of the 1st department of obstetrics and gynecology of the university of vienna were obtained vaginascopically using anaerobic transportmedia (port a cul) and were examined for aerobic and anaerobic bacteria. an average of 5.3 species was also isolated per child (2.9 aerobic and 2.5 anaerobic species). the commone ...19826121080
anaerobes: a new aetiology in cavitary pneumoconiosis.the role of mycobacteria in the cavitation of large pneumoconiotic masses is well established. in other cases softness is attributed to an ischaemic or aseptic necrosis. five cases are described in which cavitation of the pulmonary masses was caused by anaerobic bacteria, confirmed by the growth of such bacterial in cultures after transtracheal or transpleural puncture. repeated cultures for mycobacteria gave negative results. two cases were acute, having serious complications such as bronchople ...19826128024
[anaerobic sepsis]. 19836141318
[bacterial flora of the urogenital tract in men with non-gonorrheal urethritis]. 19846144143
clinical and microbiological characterization of patients with nonspecific vaginosis associated with motile, curved anaerobic rods.the vaginal secretions of 20 normal control subjects and 21 patients with motile, curved anaerobic rods were cultured for aerobic and anaerobic bacteria, chlamydia trachomatis, herpes simplex virus, and trichomonas vaginalis. extensive histories and physical examinations of the patients and microscopic appearance and gas-liquid chromatography patterns of vaginal secretions were compared between the two groups. the patients who had motile rods in their vaginal secretions more frequently presented ...19846144713
a kinetic study of the oxidative deamination of l-glutamate by peptostreptococcus asaccharolyticus glutamate dehydrogenase using a variety of coenzymes.the nad+-specific glutamate dehydrogenase from peptostreptococcus asaccharolyticus follows michaelis-menten kinetics in contrast to the enzyme from several other sources, and thus gives linear double-reciprocal plots of initial-rate data. the initial-rate parameters have been determined for this bacterial dehyrogenase in the direction of oxidative deamination. the use of alternative coenzymes leads to some conclusions about the order of substrate addition. an investigation of the ph dependence o ...19846148240
metabolic inhibition of peptostreptococcus anaerobius decreases the bactericidal effect of hydrogen peroxide.peptostreptococcus anaerobius vpi 4330-1 was tested under various conditions for examination of the bactericidal effect of hydrogen peroxide. the cells were most rapidly killed by hydrogen peroxide when they were in the exponential-growth phase. cooling or starving the cells decreased the bactericidal effect of hydrogen peroxide. the ionophore nigericin and the metal ion chelating agent 2,2'-bipyridine stopped macromolecular syntheses and greatly decreased the bactericidal effect of hydrogen per ...19816173014
[viability of anaerobic streptococci (peptostreptococci) stored in different ways]. 19836196542
gas-producing brain abscess due to peptostreptococcus. 19846204240
polyclonal b-cell activating capacities of gram-positive bacteria frequently isolated from periodontally diseased sites. 19826219204
cefoperazone therapy for obstetric and gynecologic infections.the safety and clinical efficacy of cefoperazone therapy for obstetric and gynecologic infections have been evaluated in clinical trials involving 107 women hospitalized at nine medical centers. the indications for cefoperazone therapy have included post-partum and postoperative uterine infections, pelvic inflammatory disease, postoperative pelvic infections, and miscellaneous pelvic infections. a variety of aerobic and anaerobic bacteria, including members of the genera staphylococcus, streptoc ...19836221385
[bacteriological and clinical evaluation of piperacillin in the field of obstetrics and gynecology].in vitro activity of piperacillin (pipc) against 150 recent clinical isolates from obstetric and gynecologic infections was performed in comparison with sulbenicillin (sbpc), cefmetazole (cmz), cefotiam (ctm) and cefsulodin (cfs). at 6.25 micrograms/ml pipc inhibited 93% of s. epidermidis, 86% of s. faecalis, 67% of e. coli and 80% of klebsiella. at 0.2 microgram/ml pipc inhibited 100% of peptococcus, 73% of peptostreptococcus and 100% of b. melaninogenicus group. at 3.13 micrograms/ml pipc inhi ...19826223149
[bacterial flora isolated from the cervical secretions of patients with acute gonorrhea and their sensitivities to spectinomycin and ampicillin].subsequent to studies of clinical effects of spectinomycin (spcm) and ampicillin (abpc) given in combination on acute gonorrhea in female patients, sensitivity of n. gonorrhoeae to each of these antibiotics was determined. furthermore, cervical discharge of female patients was searched for other organisms. combined antimicrobial action of spcm and abpc were also examined. aerobic and anaerobic organisms were obtained from cervical discharge of 20 cases of acute gonorrhea. the predominant aerobe ...19836228677
continuous fluorescence assay, partial purification and properties of flavokinase from megasphaera elsdenii. 19806246392
thermodynamic and electron paramagnetic resonance characterization of flavin in succinate dehydrogenase.thermodynamic parameters of succinate dehydrogenase flavin were determined potentiometrically from the analysis of free radical signal levels as a function of the oxidation-reduction potential. midpoint redox potentials of consecutive 1-electron transfer steps are -127 and -31 mv at ph 7.0. this corresponds to a stability constant of intermediate stability, 2.5 x 10(-2), which suggests flavin itself may be a converter from n = 2 to n = 1 electron transfer steps. the pk values of the free radical ...19816263883
hydrolytic enzymes of anaerobic bacteria isolated from human infections.thirty-three strains of anaerobic bacteria isolated from human clinical specimens were examined for the presence of heparinase, hyaluronidase, chondroitin sulfatase, gelatinase, collagenase, fibrinolysin, lecithinase, and lipase activities. pronounced heparinase activity was limited to species of the genus bacteroides. a number of species of the genera bacteroides and clostridium produced hyaluronidase and chondroitin sulfatase. gelatinase, collagenase, and fibrinolysin activities were encounter ...19816268657
[influence of cefotiam (sce-963, ctm) on bowel flora (author's transl)].the influence on bowel flora of ctm was studied in 5 children who were taking normal diet. 1) in the cases following no diarrhea, administration of ctm caused no significant changes in bowel flora. in the cases following diarrhea, administration of ctm caused a fall in coliform, bep group, lactobacillus and peptostreptococcus. however, after the administration was discontinued, the reduced bowel flora was returned to the normal range within a few days. 2) no overgrowth of bowel flora by pseudomo ...19816270414
growth of peptococcus and peptostreptococcus: effect of variations of culture media on efficiency of recovery.reference strains and clinical isolates of peptococcus and peptostreptococcus spp. were evaluated for their growth response in supplemented thioglycolate-yeast extract media. supplements used included various combinations of hemin, menadione, sodium bicarbonate, and tween 80. parallel studies were done to compare the efficiency of recovery of viable cells grown in thioglycolate-based media and wilkins-chalgren broth and agar. in addition, the effects of age of the medium and medium storage on vi ...19816271059
[comparison of antibacterial activity of cefmenoxime with other cephalosporins against clinically isolated bacteria (author's transl)].we examined the antibacterial activity of mx in comparison with those of other ceps, using aerobic gram-positive cocci, aerobic gram-negative bacilli and anaerobic bacteria, 870 strains in total, all isolated from clinical specimens, in 1979 and 1980. against streptococcus, cmx showed superior antibacterial activity than those of cfx, cmz, cxm and ctm. against h. influenzae, cmx also showed superior antibacterial activity than those of cfx, cmx, cxm, ctm and cez. abpc-and pipc-resistant strains ...19816278174
[a fundamental and clinical study of ceftizoxime in the field of obstetrics and gynecology (author's transl)].a fundamental and clinical study of ceftizoxime (czx), a new cephalosporin antibiotic, has brought about the following results. 1. the antibacterial activity of czx against bacteriodes fragilis and b. thetaiotaomicron was superior to that of cez, but inferior to that of cfx. czx was the most active of the 3 drugs against b. distasonis, and was as active as cfx against b. vulgatus. the mics of czx against peptococcus, peptostreptococcus and anaerobic streptococcus were 1.56 microgram/ml or lower. ...19816278175
[clinical bacteriological studies on cefotiam and cefsulodin in the field of otorhinolaryngology].clinical bacteriological studies on cefotiam and cefsulodin in the field of otorhinolaryngology were carried out and the following results were obtained. 1) aerobic and anaerobic gram-positive bacteria were dominantly isolated from the clinical materials sent to the center from the clinical institutes. 2) it was considered that streptococcus pneumoniae, haemophilus influenzae and beta-streptococcus played an important role in the primary infections in the field of otorhinolaryngology. staphyloco ...19826290705
cecal microflora of turkeys fed low or high fiber diets: enumeration, identification, and determination of cellulolytic activity.examination of cecal contents or bacterial cultures thereof from turkeys fed either a high fiber (hf) or low fiber (lf) ration indicated that direct microscopic counts of microbes were significantly higher in hf-fed than in lf-fed birds. there was no significant difference in mean colony counts between the two groups of turkeys. in both lf and hf-fed birds, 77% of the microbes were gram-positive rods, 14% gram-negative rods, and 9% gram-positive cocci. the predominant microorganism was eubacteri ...19836306633
inactivation of the polyanionic detergent sodium polyanetholsulfonate by hemoglobin.sodium polyanetholsulfonate (sps) has been added to blood culture media for many years. its incorporation results in a higher yield of positive blood cultures due to its inactivation of antimicrobial cationic compounds. the most active of these cations include complement components, aminoglycoside-aminocyclitol antibiotics, and receptors on polymorphonuclear leukocytes. there have been reports from studies conducted outside patient blood culture bottles that sps itself may possess antibacterial ...19836315765
[clinical experience with cefotiam in otorhinolaryngologic infections].clinical studies of cefotiam (ctm), a new cephalosporin derivative, in otorhinolaryngological field were performed, and the results were summarized as follows. ctm was intravenously injected to 31 cases of otorhinolaryngological infections in daily dose of 1--4 g. clinical efficacy was 33.3% in acute otitis media and chronic otitis media (acute exacerbation) (6 cases), 90% in acute tonsillitis (including peritonsillitis, peritonsillar abscess) (20 cases), 50% in acute pharyngitis (2 cases), 100% ...19836325742
[cefotiam passage into the maxillary sinus tissues].clinical study was made on cefotiam (ctm) and the following results were obtained. tissue concentrations of ctm were determined 1 hour after intravenous injection (ctm 1 g) in chronic sinusitis and maxillary cyst. concentrations of ctm were 16.53 micrograms/g, 13.26 micrograms/g in mucosa of the maxillary sinus, maxillary cyst, respectively. antibacterial activity of ctm was measured on escherichia coli, staphylococcus epidermidis, aerococcus, acidaminococcus fermentans, peptostreptococcus anaer ...19836325743
ceftriaxone: therapeutic results in various infections and kinetic studies.ceftriaxone (ro 13-9904, rocephin) was given to 67 patients suffering from 74 various infections. patients had infections of the urinary tract (36), soft tissue phlegmon (12), infections of the respiratory tract (13), osteomyelitis (7), abscesses (5) and meningitis (1). infecting organisms were e. coli (26), proteus spp. (20), p. aeruginosa (7), h. influenzae (6), enterobacter spp. (6), k. pneumoniae (3), c. freundii (2), s. marcescens (1), s. aureus (4), s. pneumoniae (2), peptostreptococcus sp ...19846329238
bacteriology of chronic tonsillitis in young adults.tonsils were obtained from 22 young adults (mean age, 23 years) suffering from chronic tonsillitis. mixed aerobic and anaerobic flora was obtained from core tonsillar cultures in all patients, yielding an average of 9.0 isolates (5.3 anaerobes and 3.7 aerobes) per specimen. the predominant anaerobic isolates were bacteroides sp, fusobacterium sp, and gram-positive cocci. the predominant aerobic isolates were alpha-hemolytic streptococci, staphylococcus aureus, branhamella catarrhalis, beta-hemol ...19846334513
frequency of occurrence and antimicrobial susceptibility of bacterial isolates from the intestinal and female genital tracts.in the selection of empiric therapy for infections of the female genital tract and intraabdominal infections in humans, the requisite information includes (1) the frequency of isolation of bacterial species dominant in the normal gastrointestinal and female genital tracts and in intraabdominal and female genital tract infections and (2) the in vitro susceptibilities of the dominant species to drugs that may be used against them (e.g., penicillin g, cefoperazone, cefoxitin, chloramphenicol, clind ...19836342102
[use of cephalosporins as antibiotic prophylaxis in cesarean section].cefoxitin, a second-generation cephalosporin, was compared with cefazolin, a first-generation cephalosporin, and a placebo in a prospective, double-blind study of antibiotic prophylaxis in women undergoing nonelective cesarean section. in the groups that received cefazolin or the placebo there eas no statistically significant change in colonization of the cervix by aerobic bacteria by the fourth day after the operation, but there was a statistically significant increase in colonization by anaero ...19836344970
the survival of anaerobic bacteria at 4 degrees c and 22 degrees c on swabs in three transport systems. statistical evaluation by application of a variance component model.the survival of anaerobic bacteria on swabs in three transport systems at 4 degrees c and 22 degrees c was evaluated. the transport systems were a charcoal-impregnated cotton swab in modified stuart's transport medium (ms), a plain cotton swab in a dry aerobic tube (da) and a plain cotton swab in an aerobic agar tube (aa). the test strains were bacteroides fragilis, b. melaninogenicus ss. intermedius, fusobacterium necrophorum, peptostreptococcus anaerobius, eubacterium lentum and clostridium ra ...19836346789
the recovery of anaerobic bacteria from swabs in three transport systems. statistical evaluation by application of a variance component model.the recovery of six anaerobic species from swabs in three transport systems was quantitatively evaluated. the test strains were bacteroides fragilis, b. melaninogenicus ss. intermedius, fusobacterium necrophorum, peptostreptococcus anaerobius, eubacterium lentum, and clostridium ramosum. the transport systems were a charcoal-impregnated cotton swab in modified stuart's transport medium (ms), or a plain cotton swab in either a dry aerobic tube (da) or an aerobic agar tube (aa). swabs were charged ...19836346790
production of histolytic enzymes by a combination of oral bacteria with known pathogenicity.eight oral bacterial strains, isolated from an infected root canal, have been investigated for their capacity to produce histolytic enzymes. the determination was performed using methods espoused by two different principles. eleven out of 12 enzymes examined were demonstrated in the "eight-strain collection". in no single bacterial strain were all enzymes revealed. it was suggested that the pathogenicity of the bacterial strains, singly or in combination, was not solely dependent on the producti ...19836352755
on the occurrence of enoate reductase and 2-oxo-carboxylate reductase in clostridia and some observations on the amino acid fermentation by peptostreptococcus anaerobius.enoate reductase present in clostridium kluyveri and clostridium spec. la 1 could be detected in three strains of c. tyrobutyricum and ten clostridia belonging to the groups of proteolytic and saccharolytic or proteolytic species, respectively. in c. pasteurianum, c. butyricum and c. propionicum enoate reductase could not be found even after growth on (e)-2-butenoate. a 2-oxo-carboxylate reductase was present in rather low activities in the non-proteolytic clostridia which produce enoate reducta ...19836354130
continuous opacity monitoring of the growth of bacteria under strict anaerobic conditions.a newly designed instrument is described which generates continuous records of the opacities of six bacterial cultures growing under strict anaerobic conditions. additions (for example, of antibacterial agents) or withdrawal of culture (for example, for viable counting) can be made at any time without breach of anaerobiosis. use of the instrument is illustrated by growth curves obtained from small inocula of two strict anaerobes, bacteroides asaccharolyticus and peptostreptococcus anaerobius and ...19836355193
the frequency and some characteristics of anaerobic bacteria isolated from various forms of bovine mastitis.the prevalence of strictly anaerobic bacteria in the secretions from untreated cases of mastitis in lactating dairy cows was investigated. the study involved 147 friesland cows in 12 highveld herds. all herds yielded cows with anaerobic udder infections. no anaerobic bacteria were recovered from cows with normal quarters or those with latent aerobic infections. only anaerobes were present in 10% of so-called 'aseptic' mastitis cases. a variety of anaerobic organisms was isolated concurrently wit ...19836355474
effects of atmosphere of incubation on recovery of bacteria and yeasts from blood cultures in tryptic soy broth.a comparison was made of the results of blood cultures between 1974 and 1981 in unvented and transiently vented bottles of tryptic soy broth under vacuum with co2. a total of 14,646 isolates were available for statistical analysis. significantly more isolates of staphylococcus epidermidis, staphylococcus aureus, bacillus, escherichia, pseudomonas, klebsiella, serratia, acinetobacter, alcaligenes, neisseria, and candida were recovered from the vented bottle. significantly more isolates of coryneb ...19836370565
anaerobic oral and dental infection.anaerobes make up a significant part of the oral and dental indigenous and pathogenic flora. their role in periodontal disease, root canal infections, infections of the hard and soft oral tissue, as well as their importance as foci for disseminated infectious disease is well established. despite the ubiquitous involvement of bacteria, significant progress in our understanding of specific microbial etiologies has occurred only in the past decade. estimates of the number of species recovered from ...19846372018
role of anaerobic bacteria in bite-wound infections.the etiologic agents usually involved in wound infections due to human or animal bites are the aerobic skin flora of the victim, e.g., staphylococcus aureus, and/or the aerobic oral flora of the biter, e.g., pasteurella multocida. while anaerobic bacteria are predominant in the normal oral flora of humans and animals, their importance in the pathogenesis of bite-wound infections has not been stressed. most investigators in this field have either not cultured these wounds for anaerobic bacteria o ...19846372026
anaerobes as normal oral flora.the human mouth provides a suitable habitat for numerous bacterial genera. anaerobic genera or genera that include anaerobic members found in the oral cavity are actinomyces, arachnia, bacteroides, bifidobacterium, eubacterium, fusobacterium, lactobacillus, leptotrichia, peptococcus, peptostreptococcus, propionibacterium, selenomonas, treponema, and veillonella. the incidence of anaerobes varies with age of the individual and with specific sites sampled. in edentulous infants, the incidence of a ...19846372039
pectinolytic enzymes of oral spirochetes from humans.five strains of obligately anaerobic, pectin-fermenting spirochetes were isolated from the subgingival plaque of humans. the strains produced two extracellular enzymatic activities that functioned in pectin degradation. one of these enzymatic activities was pectin methylesterase (ec 3.1.1.11), and the other was pectate lyase (ec 4.2.2.2) of the endo type. the data indicate that the cumulative action of these two enzymatic activities brought about depolymerization of pectin in spirochete cultures ...19846383218
clinical efficacy of cefoperazone in obstetric and gynecologic infections.compared with second-generation cephalosporins, cefoperazone, a third-generation cephalosporin, has demonstrated an extended antibacterial spectrum. in addition, it has a long serum half-life and requires only twice-a-day administration. in this study, 66 patients with clinically diagnosed obstetric and gynecologic infections were treated with cefoperazone, and 61 were evaluated for efficacy. an overall cure rate of 93% (57 patients) was achieved. in view of the excellent clinical response and c ...19846383613
[growth of anaerobes of the upper small intestine using the glovebox technic].with 80 patients a microecologic analysis of the duodenal flora has been performed. due to the optimal cultivation technique with the glove-box more frequently as supposed till now, anaerobes with partial high numbers could be detected, and classified to different types of dysbiosis. a striking role among the isolated and classified gram-positive roots play the genera eubacterium, propionibacterium, actinomyces, and bifidobacterium. peptococcus and peptostreptococcus are to be found frequently, ...19846387500
isolation of obligate anaerobic bacteria from bovine abscesses in sites other than the liver.a survey in japan showed that of 2036 slaughtered cattle 58(3%) had abscesses in sites other than the liver. in 21 of the affected animals the lesions were pulmonary and in 32 abdominal (excluding hepatic); in five animals the lesions were found elsewhere (muscle 2, skin 2, bone 1). nineteen (33%) of the 58 cattle also had abscesses in the liver. obligate anaerobes alone were isolated from 17(29%) of the affected animals (fusobacterium necrophorum 14, bacteroides spp. 2, peptostreptococcus anaer ...19846389873
[anaerobic microflora of the contents of the upper maxillary sinuses in patients with chronic sinusitis]. 19846401097
[bacteriological and clinical studies in twenty patients with thoracic empyema--significance of anaerobic bacteria]. 19836408199
susceptibility of brain to aerobic, anaerobic, and fungal organisms.the utility of an experimental animal model is dependent on its ability to simulate the actual clinical situation. with a stereotaxic injection procedure, the susceptibility of rat brain to the spectrum of organisms commonly associated with human brain abscess was determined. two strains of escherichia coli were more infective than pseudomonas aeruginosa, staphylococcus aureus, and streptococcus pyogenes. even between the e. coli strains it was possible to document significant differences in deg ...19836409808
cefamandole therapy in hand infections.hand lacerations, especially when inadequately treated, may result in infections caused by aerobic or anaerobic bacteria. anaerobic infections most commonly result from human bite injuries in which there is contact between hand and mouth. the search continues for an ideal antibiotic to employ when anaerobic organisms are suspected. in this study cefamandole, a new cephalosporin antibiotic, was employed whenever anaerobic hand infections were suspected following trauma. in each patient quantitati ...19836415153
peptostreptococcus productus strain that grows rapidly with co as the energy source.anaerobic bacteria were enriched with a sewage digestor sludge inoculum and a mineral medium supplemented with b-vitamins and 0.05% yeast extract and with a 50% co-30% n2-20% co2 (2 atm [202 kpa]) gas phase. microscopic observation revealed an abundance of gram-positive cocci, 1.0 by 1.4 micron, which occurred in pairs or chains. the coccus, strain u-1, was isolated by using roll tubes with co as the energy source. based on morphology, sugars fermented, fermentation products from glucose (h2, ac ...19846430231
[a case of septic pulmonary emboli due to peptostreptococcus associated with liver abscess]. 19846434659
diffuse sclerosing osteomyelitis of the mandible.the medical care of patients with diffuse sclerosing osteomyelitis (dso) of the mandible has been unsatisfactory. the main reasons for this have been insufficient knowledge of the disease and its natural history, difficulties in establishing the correct diagnosis, and the unknown etiology. the clinical features and natural history of the disease were studied by means of repeated radiographic and scintigraphic recordings. the biopsy technique was improved by using a slowly rotating coarse trepan ...19846437998
surgical management of piriform sinus fistula.four cases of acute cervical abscess resulting from infection through the left piriform sinus fistula are described. the fistula seems to be related to the cause of infection in many of previously reported cases of acute suppurative thyroiditis or "recurrent lateral cervical fistula." complete removal of the fistula is essential to a permanent cure, and the guide of a fogarty catheter through the fistula is useful at operation for patients with repeated infection.19846465693
anaerobic bacteria isolated from foot infections in diabetic patients: in vitro susceptibility to nine antibiotics.twenty-nine isolates of anaerobic bacteria from foot infections in diabetic patients were tested, using the agar dilution method, for in vitro susceptibility to nine antimicrobial agents. imipenem and metronidazole showed excellent activity; clindamycin and chloramphenicol were next in terms of efficacy. cefoxitin and moxalactam demonstrated good activity, although three isolates of the bacteroides fragilis group were resistant to moxalactam. penicillin g, cefotaxime, and cefoperazone showed poo ...19846467279
production of menaquinones by intestinal anaerobes.ninety intestinal organisms (71 isolates from fecal samples of neutropenic patients with cancer or from various sites in patients with intraabdominal infections and 19 control strains) were examined by reverse-phase thin-layer chromatography for their ability to produce menaquinones in vitro. menaquinones were found in all of 24 organisms of the bacteroides fragilis group. two other bacteroides species, bacteroides disiens and bacteroides bivius, also produced menaquinones. a single isolate of b ...19846470528
anaerobic ureolytic bacteria from caecal content and soft faeces of rabbit.forty strains of ureolytic bacteria were isolated from the caecal content and soft faeces of seven rabbits by the anaerobic roll tube method and were characterized. the isolates were identified with clostridium coccoides, cl. innocuum, peptostreptococcus productus, p. micros, peptococcus magnus, fusobacterium russii and fusobacterium sp. urease activity of representative strains of the various species was also determined. the study indicated that strongly-ureolytic anaerobic bacteria are present ...19846490567
peptostreptococcal empyema in akinetic mutism.peptostreptococcal empyema occurred in a 54-year-old man who had suffered from diabetes mellitus and had been in akinetic mutism. ultrasonic tomogram was useful to detect the space of empyema and to identify the decrease in volume of the empyema space with treatment.19846492491
microorganisms isolated from infected aural fistulas.aural fistula is a congenital deformity of the external ear relatively common in orientals and rare in caucasians. suppuration tends to occur, and chemotherapy rather than surgical drainage should be attempted. however, the lack of information concerning infected aural fistulas has made appropriate chemotherapy difficult. microorganisms isolated from 13 cases of infected aural fistulas were studied from january 1981 to december 1982. six species and 22 strains were isolated; nonsporeforming facu ...19846492970
induction in rats of serum agglutinins to eubacterium, peptostreptococcus and coprococcus species by the faecal flora from patients with crohn's disease and healthy subjects.sera from patients with crohn's disease more often than those from other subjects contain agglutinins against anaerobic coccoid rods, identified as peptostreptococcus productus, eubacterium contortum (two strains) and coprococcus comes. the presence of antigens of the four coccoid rods in faeces of patients with crohn's disease and healthy subjects was investigated by inoculation of rats with faecal suspensions. antigens of the coccoid rods were detected in faeces from both patients with crohn's ...19846501880
characterization of peptostreptococcus asaccharolyticus glutamate dehydrogenase purified by dye-ligand chromatography.glutamate dehydrogenase (l-glutamate:nad+ oxidoreductase (deaminating); ec 1.4.1.2) has been purified from peptostreptococcus asaccharolyticus in a single step using dye-ligand chromatography. the enzyme (gdh) was present in high yields and was stabilized in crude extracts. a subunit molecular weight of 49000 +/- 500 was determined by sds polyacrylamide gel electrophoresis and six bands were obtained after cross-linking the subunits with dimethyl suberimidate. this bacterial gdh was predominantl ...19846502134
intestinal water-soluble mucins in germfree, exgermfree and conventional animals.water-soluble intestinal mucins were investigated in germfree (gf), exgermfree (exg) and conventional (conv) rats and in gf and conv mice. after agar gel electrophoresis, all gf animals had similar specific band patterns demonstrated by pas and toluidine blue. these patterns, never seen in conv animals, disappeared in gf animals infected either with intestinal contents from conv rats or mono-infected with a mucin converting microorganism, labelled peptostreptococcus n. the intestinal microflora ...19846516849
in vitro susceptibility of anaerobic bacteria to ciprofloxacin (bay o 9867).about 80% of 70 clinical isolates of bacteroides fragilis were inhibited by 4 micrograms of ciprofloxacin (bay o 9867) per ml. the 90% mic of ciprofloxacin was 8 micrograms/ml for other bacteroides species, 2 micrograms/ml for peptococcus species, 8 micrograms/ml for peptostreptococcus species, and 16 micrograms/ml for clostridium and eubacterium species.19846517561
suppurative peritonitis in cats associated with anaerobic bacteria. 19846529399
[vertebral osteomyelitis caused by anaerobes. a new case]. 19846531485
purification and characterization of nadp-dependent 7 beta-hydroxysteroid dehydrogenase from peptostreptococcus productus strain b-52.an nadp-dependent 7 beta-hydroxysteroid dehydrogenase was purified 11.5-fold over the activity in crude cell extracts prepared from peptostreptococcus productus strain b-52, by using sephadex g-200 and deae-cellulose column chromatography. 7 beta-dehydrogenation was the sole transformation of bile acids catalyzed by the partially purified enzyme. the enzyme preparation (spec. act. 2.781 iu per mg protein) had an optimum ph of 9.8. lineweaver-burk plots showed a michaelis constant (km) value of 0 ...19836572075
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