PMID(sorted descending)
management of complex multi-space odontogenic infections.the successful management of multi-space orofacial odontogenic infections involves identification of the source of the infection, the anatomical spaces encountered, the predominant microorganisms that are found during the various stages of odontogenic fascial space infection, the impact of the infectious process on defense systems, the ability to use and interpret laboratory data and imaging studies, and a thorough understanding of contemporary antibiotic and supportive care. the therapeutic goa ...200212572406
the microflora recovered from the outer-surfaces of the frialit-2 implanto-prosthetic connector.the aim of the present investigation was to examine the outer-surface microbiota of the prosthetic connector of frialit-2 implants, and to compare the microbial findings with the peri-implant parameters 2 years after functional loading. in 16 implant-treated patients (55.8 +/- 9.5 years) the outer-surface micro-organisms of the prosthetic connectors were determined in 32 frialit-2 implants. the functional loading time of the prosthetic suprastructures was 24.1 +/- 13.8 months on average. after r ...200312562362
acute bacterial suppurative parotitis: microbiology and management.the parotid gland is the salivary gland most commonly affected by inflammation. the most common pathogens associated with acute bacterial parotitis are staphylococcus aureus and anaerobic bacteria. the predominant anaerobes include gram-negative bacilli (including pigmented prevotella and porphyromonas spp.), fusobacterium spp., and peptostreptococcus spp. streptococcus spp. (including s. pneumoniae) and gram-negative bacilli (including escherichia coli) have also been reported. gram-negative or ...200312544218
dna from periodontopathogenic bacteria is immunostimulatory for mouse and human immune cells.although bacterial dna (bdna) containing unmethylated cpg motifs stimulates innate immune cells through toll-like receptor 9 (tlr-9), its precise role in the pathophysiology of diseases is still equivocal. here we examined the immunostimulatory effects of dna extracted from periodontopathogenic bacteria. a major role in the etiology of periodontal diseases has been attributed to actinobacillus actinomycetemcomitans, porphyromonas gingivalis, and peptostreptococcus micros. we therefore isolated d ...200312540566
[the microorganisms associated with bacterial vaginosis as a cause of tubal sterility].bacterial vaginosis is one of the most widespread disturbances of the normal state of the vagina of women of reproductive and menopausal age. this condition is highly disregarded due to its moderate and not always clear clinical manifestation. the disturbed proportion of microorganisms in vagina is shown in the strong decrease or lack of lactobacilli and a spread of associated with bacterial vaginosis microorganisms. g. vaginalis, mobiluncis spp., bacteroides spp. (without b. fragilis), peptostr ...200212516260
comparison of ligand polarization and enzyme activation in medium- and short-chain acyl-coenzyme a dehydrogenase-novel analog complexes.spectroelectrochemical and off-resonance raman indicate that substrate/product binding to medium-chain acyl-coenzyme a (coa) dehydrogenase (pmcad) results in ligand polarization and positive flavin potential shifts, which activate the enzyme for electron transfer. bacterial short-chain acyl-coa dehydrogenase (bscad) typically exhibits smaller potential shifts upon substrate/product binding that have not been linked to ligand polarization. to further investigate the roles of ligand binding and po ...200312504892
metabolism of pyrrolizidine alkaloids by peptostreptococcus heliotrinreducens and a mixed culture derived from ovine ruminal fluid.a mixed culture of ovine ruminal microbes metabolizes the macrocyclic pyrrolizidine alkaloids present in the plant senecio jacobaea, including jacobine and seneciphylline. previous attempts to identify metabolites of these alkaloids have not been successful. the objective of this study was to compare the metabolism of pyrrolizidine alkaloids by a mixed culture of ovine ruminal microbes to the metabolism of pyrrolizidine alkaloids by the known organism peptostreptococcus heliotrinreducens. p. hel ...200212488016
development of a membrane-array method for the detection of human intestinal bacteria in fecal samples.a membrane-array method was developed for the detection of human intestinal bacteria in fecal samples without using the expensive microarray-arrayer and laser-scanner. the 16s rdna sequences of 20 predominant human intestinal bacterial species were used to design oligonucleotide probes. three 40-mer oligonucleotides specific for each bacterial species (total 60 probes) were synthesized and applied to nitrocellulose membranes. digoxigenin (dig)-labeled 16s rdnas were amplified by polymerase chain ...200212477438
porphyromonas gingivalis, bacteroides forsythus and other putative periodontal pathogens in subjects with and without periodontal destruction.bacteria play an essential role in the pathogenesis of destructive periodontal disease. it has been suggested that not all bacteria associated with periodontitis may be normal inhabitants of a periodontally healthy dentition. in particular, porphyromonas gingivalis and actinobacillus actinomycetemcomitans have been isolated infrequently from subjects without periodontitis. the aim of the present study was to compare prevalence and proportions of a number of periodontal bacteria in periodontitis ...200212472995
development of a pcr assay specific for peptostreptococcus anaerobius.peptostreptococcus anaerobius is a gram-positive anaerobic coccus that is widely distributed in the normal human flora. the organism has also been implicated as a causative agent of several systemic infections, including endocarditis and infections of the genitourinary and gastrointestinal tracts. its role in oral disease is less well defined, although it has been implicated in periodontal disease, gingivitis and root canal infections. identification of p. anaerobius in clinical samples is curre ...200212466408
comparison of 16s rdna-based pcr and checkerboard dna-dna hybridisation for detection of selected endodontic pathogens.molecular methods have been used recently to investigate the bacteria encountered in human endodontic infections. the aim of the present study was to compare the ability of a 16s rdna-based pcr assay and checkerboard dna-dna hybridisation in detecting actinobacillus actinomycetemcomitans, bacteroides forsythus, peptostreptococcus micros, porphyromonas endodontalis, por. gingivalis and treponema denticola directly from clinical samples. specimens were obtained from 50 cases of endodontic infectio ...200212466407
identification of bacteria in acute endodontic infections and their antimicrobial susceptibility.the purpose of the study was to identify the bacterial composition of the microbiota from acute endodontic abscesses/cellulitis and their antimicrobial susceptibilities.200212464902
antibacterial activity of moxifloxacin against periodontal anaerobic pathogens involved in systemic infections.the in vitro activity of moxifloxacin was compared with that of penicillin g, amoxycillin/clavulanate, cefoxitin, erythromycin, clindamycin and metronidazole against 158 isolates associated with periodontal infections. mic(50)/mic(90) values of moxifloxacin were respectively 0.06/0.5 mg/l for porphyromonas gingivalis (35), for prevotella spp. (28) and actinomyces spp. (35), 0.12/0.25 mg/l for fusobacterium nucleatum (20) and 0.06/0.12 mg/l for peptostreptococcus spp. (30). the minimum inhibitory ...200212458140
ertapenem (mk-0826), a new carbapenem: comparative in vitro activity against clinically significant anaerobes.ertapenem, a new long acting beta-lactam with broad-spectrum antimicrobial coverage, was tested in vitro to compare its activity against 556 clinical isolates of anaerobes to other established agents using a broth microdilution method as recommended by the nccls. against all anaerobes ertapenem inhibited 99.1% of isolates at 4 microg/ml, had a mode mic of 0.12 microg/ml, and showed activity comparable to imipenem, meropenem, trovafloxacin, piperacillin-tazobactam, and metronidazole. five of the ...200212458126
bacteriology of acute and chronic sphenoid sinusitis.aspirates of 16 acutely infected and 7 chronically infected sphenoid sinuses were processed for aerobic and anaerobic bacteria. a total of 29 isolates were recovered from the 16 cases of acute sphenoid sinusitis (1.8 per specimen): 22 aerobic and facultative (1.4 per specimen), and 7 anaerobic (0.4 per specimen). aerobic and facultative organisms alone were recovered in 10 specimens (62%), anaerobes alone were isolated in 3 (19%), and mixed aerobic and anaerobic bacteria were recovered in 3 (19% ...200212450174
heterogeneity within the gram-positive anaerobic cocci demonstrated by analysis of 16s-23s intergenic ribosomal rna polymorphisms.peptostreptococci are gram-positive, strictly anaerobic bacteria which, although regarded as members of the commensal human microflora, are also frequently isolated from sites of clinical infection. the study of this diverse group of opportunist pathogens has been hindered by an inadequate taxonomy and the lack of a valid identification scheme. recent re-classification of the peptostreptococcus family into five distinct genus groups has helped to clarify the situation. however, this has been on ...200212448679
two-part implants inserted in a one-stage or a two-stage procedure. a prospective comparative study.the aim of this study was to evaluate the feasibility of using a two-part implant system in a one-stage procedure and to monitor the microflora in the peri-implant area in relation to clinical and radiographic outcome.200212445222
prevalence of pathogens in diabetic foot infection in south indian type 2 diabetic determine the prevalence of pathogens in diabetic foot infections, in relation to parameters like wagner's grading, duration of diabetes and healing time.200212421021
a study on the isolation of anaerobic bacteria from the root canal infections in anterior non-vital teeth with special reference to bacteroides species. 200212420575
phenolic antibacterials from piper betle in the prevention of halitosis.piper betle l. (piperaceae) leaves which are traditionally used in india and china in the prevention of oral malodor was examined by bioassay-guided fractionation to yield allylpyrocatechol (apc) as the major active principle which showed promising activity against obligate oral anaerobes responsible for halitosis. the biological studies with apc indicated that the potential to reduce methylmercaptan and hydrogen sulfide was mainly due to the anti-microbial activity as established using dynamic ...200212413722
comparative in vitro activities of ertapenem against bacterial pathogens from patients with acute pelvic infection.this study compared the in vitro activities of ertapenem, ceftriaxone, co-amoxiclav, ciprofloxacin and piperacillin-tazobactam against 314 aerobic bacteria and of ertapenem, piperacillin-tazobactam, cefoxitin, ceftriaxone, chloramphenicol, ticarcillin-clavulanate, ampicillin-sulbactam, clindamycin and metronidazole against 500 anaerobic bacteria from 212 patients with acute pelvic infection. antimicrobial susceptibilities were determined by broth microdilution (aerobes) or agar dilution (anaerob ...200212407133
joint and bone infections due to anaerobic bacteria in children.the current review describes the microbiology, diagnosis and management of septic arthritis and osteomyelitis due to anaerobic bacteria in children. staphylococcus aureus, haemophilus influenzae type-b, and group a streptococcus, streptococcus pneumoniae, kingela kingae, neisseria meningiditis and salmonella spp are the predominant aerobic bacteria that cause arthritis in children. gonococcal arthritis can occur in sexually active adolescents. the predominant aerobes causing osteomyelitis in chi ...200212396847
recovery of anaerobic bacteria from a glossal abscess in an adolescent.glossal abscess is infrequent in children. anaerobic bacteria are rarely recovered from this infection and never have been reported in children or adolescents. a 15-year-old patient presented with a tongue abscess following trauma. aspirate of the abscess yielded polymicrobial anaerobic flora: prevotella melaninogenica, fusobacterium nucleatum, and peptostreptococcus micros. the patient recovered following incision and drainage and 14 days of antimicrobial therapy with clindamycin. this report i ...200212395007
in vitro activities of faropenem against 579 strains of anaerobic bacteria.the activity of faropenem, a new oral penem, was tested against 579 strains of anaerobic bacteria by using the nccls-approved reference method. drugs tested included amoxicillin-clavulanate, cefoxitin, clindamycin, faropenem, imipenem, and metronidazole. of the 176 strains of bacteroides fragilis group isolates tested, two isolates had faropenem mics of 64 micro g/ml and imipenem mics of >32 micro g/ml. faropenem had an mic of 16 micro g/ml for an additional isolate of b. fragilis; this strain w ...200212384389
sequence variations within protein families are linearly related to structural is commonly believed that similarities between the sequences of two proteins infer similarities between their structures. sequence alignments reliably recognize pairs of protein of similar structures provided that the percentage sequence identity between their two sequences is sufficiently high. this distinction, however, is statistically less reliable when the percentage sequence identity is lower than 30% and little is known then about the detailed relationship between the two measures of s ...200212381308
initial effect of controlled release chlorhexidine on subgingival microorganisms.little or no data exist on the ability of subgingival application of periochip (2.5 mg chlorhexidine gluconate in a biodegradable chip; astra pharmaceuticals, westborough, ma, usa) to suppress periodontopathic microorganisms. the present study compared the subgingival microbiota of periodontitis sites receiving the chlorhexidine chip plus scaling and root planing (sc/rp) or sc/rp alone.200212366861
[a throat infection with rapid deterioration]. 200212362714
antimicrobial susceptibility of major pathogens of orofacial odontogenic infections to 11 beta-lactam this study, we evaluated the current effectiveness of 11 beta-lactam antibiotics for treatment of orofacial odontogenic infections by determining the antimicrobial susceptibility of the major pathogens. the antimicrobial susceptibilities of viridans streptococci (n = 47), peptostreptococcus (n = 67), porphyromonas (n = 18), fusobacterium (n = 57), black-pigmented prevotella (n = 59) and non-pigmented prevotella (n = 47) isolated from pus specimens of 93 orofacial odontogenic infections to pen ...200212354209
evaluation of serological markers to differentiate between ulcerative colitis and crohn's disease: panca, asca and agglutinating antibodies to anaerobic coccoid rods.accurate diagnosis of inflammatory bowel disease, in particular the differentiation between ulcerative colitis and crohn's disease, is important for treatment and prognosis. several serological markers have been used as non-invasive diagnostic tools in inflammatory bowel disease patients both to differentiate ulcerative colitis from crohn's disease and to define patient subgroups.200212352222
protein l mutants for the crystallization of antibody many cases, antibody and their complexes can be crystallized and their structure determined without major difficulties. the remaining problematic cases may be approached through techniques such as of combinatorial complex crystallization which uses immunoglobulin binding proteins (ibp). the range of lattices that can be made using this method can be expanded by engineering mutants of ibp domains. we have designed peptostreptococcus magnus protein l (ppl) mutants with altered immunoglobulin li ...200212351898
[a comparative study of the various organisms causing salpingitis and their potential presence in iud wearers].a prospective study was undertaken to compare the enbdometrial and vaginal bacteria of a continuous series of 283 patients who had iuds removed between june 1, 1979 and june 1, 1983, by the same clinician. measures were taken to avoid contamination of the iud upon removal by the vaginal bacteria, but as a practical matter it was impossible to avoid all contact. the iuds involved were multiload copper 250 or 375, gyne t and t 200, nova t, and dimelys. the number of positive cultures from the ...198512339972
evidence for plasticity and structural mimicry at the immunoglobulin light chain-protein l interface.the multidomain bacterial surface protein l (ppl) is a virulence factor expressed by only 10% of peptostreptococcus magnus strains, and its expression is correlated with bacterial vaginosis. the molecular basis for its ability to recognize 60% of mammalian immunoglobulin light chain variable regions (v(l)) has been described recently by x-ray crystallography, which suggested the presence of two v(l) binding sites on each protein l domain (graille, m., stura, e. a., housden, n. g., beckingham, j. ...200212221088
[endogenous intragastrical microflora in gastric cancer]the aim of this prospective study was to identify the type of microbial flora and additionally to correlate the total number of microorganisms with factors such as age, tumoral area, and the ph gastric in unaffected gastric mucosal biopsies obtained during gastrojejunostomy (n=5) or gastrectomy (n=1) in patients with gastric adenocarcinoma (n=15) or lymphoma (n=1) at the belen hospital, trujillo, peru, between january 1, 1996 and april 30,1998. the mean age of the patients was 54.4 + 18.3 years ...199812209218
[anaerobic bacterial analysis of 436 specimens of clinical infection].this study was aimed at the relationship between anaerobes and clinical infection. aerobic and anaerobic bacterial culture was performed for 436 specimens of clinical infection. the incidence of anaerobic bacteria isolates and predominant species were analyzed. the results showed that the incidence of anaerobic isolates was 88.3% (385/436), the main predeminoue species were gram negative nonsporeforming anaerobic rods (bacteroids, prevotella and porphyromonas) and peptostreptococcus. the endogen ...199912205907
comparative in vitro activity of faropenem and 11 other antimicrobial agents against 405 aerobic and anaerobic pathogens isolated from skin and soft tissue infections from animal and human bites.faropenem, a new oral beta-lactam agent with a penem structure, was very active against 405 aerobic and anaerobic bite isolates. it inhibited 232 of 236 (98%) aerobic isolates, including all pasteurella spp. and eikenella corrodens at < or = 0.25 mg/l, and 164/169 (97%) anaerobic isolates, at < or = 1 mg/l. the 10 isolates that required > or = 2 mg/l for inhibition were one strain each of acinetobacter lwoffi, corynebacterium minutissimum, bacteroides ovatus, lactobacillus delbrueckii and peptos ...200212205068
genotypic diversity of clinical actinomyces species: phenotype, source, and disease correlation among genospecies.we determined the frequency distribution of actinomyces spp. recovered in a routine clinical laboratory and investigated the clinical significance of accurate identification to the species level. we identified 92 clinical strains of actinomyces, including 13 strains in the related arcanobacterium-actinobaculum taxon, by 16s rrna gene sequence analysis and recorded their biotypes, sources, and disease associations. the clinical isolates clustered into 21 genogroups. twelve genogroups (74 strains) ...200212202591
combinations of bacterial species in endodontic infections.this study was undertaken to investigate combinations of bacteria found in root-canal infections of teeth with periapical bone destruction without clinical signs and symptoms.200212196223
randomized, double-blind, multicenter comparison of oral cefditoren 200 or 400 mg bid with either cefuroxime 250 mg bid or cefadroxil 500 mg bid for the treatment of uncomplicated skin and skin-structure infections.uncomplicated skin and skin-structure infections are commonly observed in medical practice. because these infections typically are confined to the superficial layers and seldom lead to the destruction of skin structures and resultant systemic dissemination, in general they can be treated with an oral antibiotic with potent microbiologic activity against gram-positive pathogens.200212182257
design and evaluation of oligonucleotide-microarray method for the detection of human intestinal bacteria in fecal oligonucleotide-microarray method was developed for the detection of intestinal bacteria in fecal samples collected from human subjects. the 16s rdna sequences of 20 predominant human intestinal bacterial species were used to design oligonucleotide probes. three 40-mer oligonucleotides specific for each bacterial species (total 60 probes) were synthesized and applied to glass slides. cyanine5 (cy5)-labeled 16s rdnas were amplified by polymerase chain reaction (pcr) from human fecal samples or ...200212167534
clinical review: bacteremia caused by anaerobic bacteria in children.this review describes the microbiology, diagnosis and management of bacteremia caused by anaerobic bacteria in children. bacteroides fragilis, peptostreptococcus sp., clostridium sp., and fusobacterium sp. were the most common clinically significant anaerobic isolates. the strains of anaerobic organisms found depended, to a large extent, on the portal of entry and the underlying disease. predisposing conditions include: malignant neoplasms, immunodeficiencies, chronic renal insufficiency, decubi ...200212133179
cutaneous and subcutaneous infections in newborns due to anaerobic bacteria.this review describes the microbiology and management of the major cutaneous and subcutaneous infections in newborns where anaerobic bacteria predominate: omphalitis, necrotizing fasciitis, breast abscess, and scalp infection following intrauterine fetal monitoring. the predominant bacteria known to cause these infections are group b streptococcus, group d enterococcus, group a streptococcus, staphylococcus aureus, enterobacteriaceae, and anaerobic bacteria. all of these agents can colonize or i ...200212122901
actinobacillus actinomycetemcomitans proportion of subgingival bacterial flora in relation to its clonal type.we investigated whether certain actinobacillus actinomycetemcomitans clones occur in elevated proportions in subgingival flora, and if the proportions relate to other bacteria in the samples. a total of 121 a. actinomycetemcomitans strains from 121 patients with periodontitis were serotyped and 60 strains were also genotyped. the 121 strains were divided into three groups and the 60 strains into two groups according proportion of a. actinomycetemcomitans. the samples from the 60 patients with ge ...200212120706
bacterial superinfection in human tungiasis.tungiasis is caused by penetration of the female sand flea tunga penetrans into the epidermis of its host. it is endemic in many countries in latin america, the caribbean and sub-saharan africa. although superinfection is a common clinical observation, the frequency and the pattern of bacterial pathogens associated with tungiasis have never been investigated systematically. we conducted a prospective clinico-bacteriological study with patients living in a shantytown in fortaleza, capital of cear ...200212100437
invasion of dentinal tubules by oral bacteria.bacterial invasion of dentinal tubules commonly occurs when dentin is exposed following a breach in the integrity of the overlying enamel or cementum. bacterial products diffuse through the dentinal tubule toward the pulp and evoke inflammatory changes in the pulpo-dentin complex. these may eliminate the bacterial insult and block the route of infection. unchecked, invasion results in pulpitis and pulp necrosis, infection of the root canal system, and periapical disease. while several hundred ba ...200212097359
peptostreptococcal pericarditis complicating anti-tumour necrosis factor alpha treatment in rheumatoid arthritis. 200212079913
[yearly changes in antibacterial activities of cefozopran against various clinical isolates between 1996 and 2000--i. gram-positive bacteria].the in vitro antibacterial activities of cefozopran (czop), an agent of cephems, against various clinical isolates obtained between 1996 and 2000 were yearly evaluated and compared with those of other cephems, oxacephems, carbapenems, and penicillins. fifteen species, 1,062 strains, of gram-positive bacteria were isolated from the clinical materials annually collected from january to december, and consisted of methicillin-susceptible staphylococcus aureus (mssa; n = 127), methicillin-resistant s ...200212071093
[sensitivity of the folding pathway to the details of amino acid sequence].experimental data on the structure of transition state demonstrate that the proteins with the same topology as a rule have similar folding nuclei (the structured formed part of the transition state). in this review we discuss the experimental works which show that the position of folding nuclei is different among proteins with the same topology. these facts underline that the folding pathway is sensitive to the details of amino-acid sequence.200212068621
[clinical aspects of abscess development in parotitis].the purpose of this paper was to analyse parotideal abscesses on the basis of a larger number of patients.200212063691
effect of deuteration on some structural parameters of methyl groups in proteins as evaluated by residual dipolar bond methyl 1h-13c and 13cmethyl-13c scalar and residual dipolar couplings have been measured at sites in an 15n, 13c, approximately 50% 2h labeled sample of the b1 immunoglobulin binding domain of peptostreptococcal protein l to investigate changes in the structure of methyl groups in response to deuterium substitution. both one bond methyl 1h-13c and 13cmethyl-13c scalar coupling constants have been found to decrease slightly with increasing deuterium content. previous studies have shown t ...200212061716
reclassification of clostridium hydroxybenzoicum as sedimentibacter hydroxybenzoicus gen. nov., comb. nov., and description of sedimentibacter saalensis sp. nov..strain zf2t, isolated from freshwater sediment, is a motile, rod-shaped, gram-positive, endospore-forming, amino acid- and pyruvate-utilizing, anaerobic bacterium. it requires yeast extract for growth. carbohydrates are not utilized. the optimal temperature and ph for growth are 37 degrees c and 6.8-7.3, respectively. the g+c content of the dna is 34.0 mol %. a 16s rdna sequence analysis of strain zf2t revealed that the highest similarity (94.4%) was shared with clostridium hydroxybenzoicum jw/z ...200212054241
deuterium spin probes of side-chain dynamics in proteins. 2. spectral density mapping and identification of nanosecond time-scale side-chain the previous paper in this issue we have demonstrated that it is possible to measure the five different relaxation rates of a deuteron in (13)ch(2)d methyl groups of (13)c-labeled, fractionally deuterated proteins. the extensive set of data acquired in these experiments provides an opportunity to investigate side-chain dynamics in proteins at a level of detail that heretofore was not possible. the data, acquired on the b1 domain of peptostreptococcal protein l, include 16 (9) relaxation measu ...200212033876
deuterium spin probes of side-chain dynamics in proteins. 1. measurement of five relaxation rates per deuteron in (13)c-labeled and fractionally (2)h-enriched proteins in pulse sequences are presented for the measurement of the relaxation of deuterium double quantum, quadrupolar order, and transverse antiphase magnetization in (13)ch(2)d methyl groups of (15)n-, (13)c-labeled, fractionally deuterated proteins. together with previously developed experiments for measuring deuterium longitudinal and transverse decay rates [muhandiram, d. r.; yamazaki, t.; sykes, b. d.; kay, l. e. j. am. chem. soc. 1995, 117, 11536], these schemes allow measurement of the five un ...200212033875
physical and genetic map of the finegoldia magna (formerly peptostreptococcus magnus) atcc 29328 genome.a physical and genetic map of finegoldia magna (formerly peptostreptococcus magnus) atcc 29328 chromosome was constructed. the order of rare cleavage restriction fragments was determined by double digestion with the restriction enzymes i-ceui, sgrai, apai and pmei, cross-hybridization and apai-linking clones. the size of the circular chromosome of f. magna was estimated to be 1.9 mb. this strain also had a 200-kb megaplasmid. the chromosome contained four rrn operons, and the orientation of two ...200212023074
aerobic and anaerobic microbiology of suppurative sialadenitis.aspirates of pus from acute suppurative sialadenitis were investigated for the presence of aerobic and anaerobic bacteria. a total of 47 specimens, 32 from parotid, 9 from submandibular and 6 from sublingual glands yielded bacterial growth. fifty five isolates, 25 aerobic and 30 anaerobic, were isolated from parotid infection: anaerobic bacteria only were detected in 13 (41%) specimens, aerobic or facultative bacteria only in 11 (34%) and mixed aerobic and anaerobic bacteria in 8 (25%). of a tot ...200212018662
mice lacking monocyte chemoattractant protein 1 have enhanced susceptibility to an interstitial polymicrobial infection due to impaired monocyte recruitment.monocyte chemoattractant protein 1 (mcp-1) is an important chemokine that induces monocyte recruitment in a number of different pathologies, including infection. to investigate the role of mcp-1 in protecting a host from a chronic interstitial polymicrobial infection, dental pulps of mcp-1(-/-) mice and controls were inoculated with six different oral pathogens. in this model the recruitment of leukocytes and the impact of a genetic deletion on the susceptibility to infection can be accurately a ...200212011011
parotid salivary s-iga antibodies during experimental gingivitis in smokers and non-smokers.persons who smoke display a less pronounced increase of gingival bleeding in the experimental gingivitis model as compared with non-smokers. the aim of the present study was to investigate whether this could partly be explained by differences in levels of parotid total secretory iga (s-iga) or parotid s-iga reactive with selected oral microorganisms. parotid saliva samples were obtained from 11 smoking and 14 non-smoking volunteers, at baseline, after 5 and 14 days of full mouth experimental gin ...200212009188
the effects of an immediately pre-surgical chlorhexidine oral rinse on the bacterial contaminants of bone debris collected during dental implant implant surgery produces bone debris that can be used in the "simultaneous augmentation" technique. although this debris is contaminated with oral bacteria, a stringent aspiration protocol has been shown to reduce the levels of contamination. chlorhexidine mouthrinse is a well-proven antibacterial rinse that has been shown to reduce infectious complications associated with dental implants. this study examined the effect of pre-operative rinsing with a 0.1% chlorhexidine digluconate mouthr ...200212005141
in vitro bactericidal activity and post-antibiotic effect of abt-773 versus co-amoxiclav against anaerobes. 200212003989
bacteriology of acute and chronic frontal sinusitis.aspirates of 15 acutely and 13 chronically infected frontal sinuses were processed for aerobic and anaerobic bacteria. a total of 20 isolates (1.3 per specimen) were recovered from the 15 cases of acute frontal sinusitis, 16 aerobic and facultative isolates (1.1 per specimen) and 4 anaerobic isolates (0.3 per specimen). aerobic and facultative organisms alone were recovered in 13 specimens (87%), and mixed aerobic and anaerobic bacteria were recovered in 2 (13%). the predominant aerobic and facu ...200212003592
quantitative microbiological study of human carious dentine by culture and real-time pcr: association of anaerobes with histopathological changes in chronic pulpitis.the bacteria found in carious dentine were correlated with the tissue response of the dental pulps of 65 teeth extracted from patients with advanced caries and pulpitis. standardized homogenates of carious dentine were plated onto selective and nonselective media under anaerobic and microaerophilic conditions. in addition, real-time pcr was used to quantify the recovery of anaerobic bacteria. primers and fluorogenic probes were designed to detect the total anaerobic microbial load, the genera pr ...200211980945
[post-marketing surveillance of antibacterial activities of cefozopran against various clinical isolates--i. gram-positive bacteria].as a post-marketing surveillance, the in vitro antibacterial activities of cefozopran (czop), an agent of cephems, against various clinical isolates were yearly evaluated and compared with those of other cephems, oxacephems, penicillins, and carbapenems. changes in the bacterial sensitivity for czop were also evaluated with the resistance ratio calculated with breakpoint mic. sixteen species (1,913 strains) of gram-positive bacteria were isolated from the clinical materials annually collected fr ...200211977919
treatment of papillon-lefèvre syndrome periodontitis.conventional mechanical treatment of papillon-lefèvre syndrome periodontitis has a poor prognosis. this report describes an effective antimicrobial treatment of rapidly progressing periodontitis in an 11-year old girl having papillon-lefèvre syndrome.200211966936
bacteriology and antimicrobial susceptibility of gram-positive cocci isolated from pus specimens of orofacial odontogenic infections.we recently reported the beta-lactamase production and antimicrobial susceptibility of anaerobic gram-negative rods isolated from pus specimens of 93 orofacial odontogenic infections. in this report, we determine the bacteriology and antimicrobial susceptibility of bacteria other than anaerobic gram-negative rods, mainly gram-positive cocci, isolated from the same specimens. streptococcus constellatus and peptostreptococcus micros were frequent isolates from all types of infection examined. pept ...200211929563
management of acute suppurative parotitis. 200211928106
acute suppurative thyroiditis caused by an infected piriform sinus fistula with thyrotoxicosis.we report herein an unusual case of thyrotoxicosis caused by acute suppurative thyroiditis (ast) infected through a piriform sinus fistula (psf). a 28-year-old man presented with pain over the thyroid gland and elevated serum thyroid hormone levels, a picture similar to subacute thyroiditis. a fine-needle aspiration biopsy from the left lobe showed neutrophil infiltration, and culture from the aspirate grew anaerobic peptostreptococcus. a neck computed tomography (ct) scan showed an abscess in t ...200211916288
[four cases of non-clostridial gas gangrene with diabetes mellitus].we report four cases of non-clostridial gas gangrene. all cases were associated with diabetes mellitus as the underlying disease. case 1: a 60-year-old male developed an ulcerative lesion on the dorsum of his left foot. peptostreptococcus asaccharolyticus, citrobacter freundii and staphyrococcus epidermidis were identified in culture from odoriferous pus. case 2: a 81-year-old female developed a lesion on her vulva spreading to the right lower abdomen. bacteroides bivius, peptostreptococcus asac ...200211915237
bacterial meningoencephalomyelitis in dogs: a retrospective study of 23 cases (1990-1999).the clinical records of 23 dogs (1990-1999) with histopathologically confirmed bacterial meningoencephalomyelitis were evaluated retrospectively. no breed, age, sex, or weight predisposition was found. all the dogs presented with clinical signs of a brain lesion, whereas 5 of 23 had neck pain. pyrexia was detected in 11 of 23 dogs on admission. cbcs revealed neutrophilic leucocytosis in 7 of 21 dogs and thrombocytopenia in 3 of 21 dogs. the serum chemistry profiles were abnormal in 15 of 21 dogs ...200211899031
meningitis and shunt infection caused by anaerobic bacteria in children.this review describes the microbiology and management of meningitis and shunt infections caused by anaerobic bacteria in children. the predominant anaerobes recovered in meningitis are bacteriodes spp., bacteriodes fragilis, fusobacterium spp., and clostridium spp. peptostreptococcus, veillonella, actinomyces, propionibacterium acnes, and eubacterium are less commonly isolated. the predisposing conditions for meningitis are acute or chronic middle-ear infection, sinusitis, pharyngitis, and pulmo ...200211897473
subgingival microbiota of indigenous indians of central define the subgingival microbial profiles of adult subjects from a previously identified rural community of indigenous indians in guatemala, central america.200211895544
maternal periodontitis and prematurity. part ii: maternal infection and fetal exposure.clinical data from the first 812 deliveries from a cohort study of pregnant mothers entitled oral conditions and pregnancy (ocap) demonstrate that both antepartum maternal periodontal disease and incidence/progression of periodontal disease are associated with preterm birth and growth restriction after adjusting for traditional obstetric risk factors. in the current study we present measures of maternal periodontal infection using whole chromosomal dna probes to identify 15 periodontal organisms ...200111887461
differences in backbone dynamics of two homologous bacterial albumin-binding modules: implications for binding specificity and bacterial adaptation.proteins g and pab are bacterial albumin-binding proteins expressed at the surface of group c and g streptococci and peptostreptococcus magnus, respectively. repeated albumin-binding domains, known as ga modules, are found in both proteins. the third ga module of protein g from the group g streptococcal strain g148 (g148-ga3) and the second ga module of protein pab from p.magnus strain alb8 (alb8-ga) exhibit 59% sequence identity and both fold to form three-helix bundle structures that are very ...200211884146
periodontal bacteria in rabbit mandibular and maxillary abscesses.despite the high incidence of odontogenic abscesses in pet rabbits, published data on the bacteriology of these infections are lacking, and clinical cultures are often ambiguous, making antibiotic choices difficult. in order to define the bacteriology of these infections, 12 rabbit mandibular and maxillary abscesses were cultured aerobically and anaerobically. all specimens yielded pathogenic bacteria, including fusobacterium nucleatum, prevotella heparinolytica, prevotella spp., peptostreptococ ...200211880435
bacteriocin-like activity of butyrivibrio fibrisolvens jl5 and its effect on other ruminal bacteria and ammonia production.when ruminal bacteria from a cow fed hay were serially diluted into an anaerobic medium that had only peptides and amino acids as energy sources, little growth or ammonia production was detected at dilutions greater than 10(-6). the 10(-8) and 10(-9) dilutions contained bacteria that fermented carbohydrates, and some of these bacteria inhibited clostridium sticklandii sr, an obligate amino acid-fermenting bacterium. phylogenetic analysis indicated that the most active isolate (jl5) was closely r ...200211872448
[the microflora of ulzerous zone mucosa in patients with duodenal ulcer].bacteriological study of the biopsies taken from gastric and duodenal mucosa of 10 healthy volunteers and 74 patients with duodenal ulcer, was carried out. in the gastroduodenal zone of healthy subjects microorganisms of 6 genera (streptococcus, candida, staphylococcus, bacillus, helicobacter and lactobacillus) were detected. h. pylori was isolated in 20% of cases only in biopsy specimens taken from the antral section of the stomach of healthy as monoculture or in combination with c. albicans. i ...200111871291
antimicrobial activity of sch 27899, oligosaccharide member of the everninomycin class with a wide gram-positive spectrum.the in vitro antimicrobial activity of sch 27899 (everninomycin), a novel oligosaccharide compound of the everninomycin class, was compared with vancomycin, chloramphenicol, clinafloxacin, teicoplanin and doxycycline against 428 clinical strains of bacteria. everninomycin base exhibited the greatest antimicrobial activity compared to other formulations against all strains tested (mic90: 0.25 microg/ml) followed by clinafloxacin and teicoplanin (mic90: 0.5 microg/ml), vancomycin (mic90: 2 microg/ ...199511866719
in vitro activity of gemifloxacin compared to seven other oral antimicrobial agents against aerobic and anaerobic pathogens isolated from antral sinus puncture specimens from patients with sinusitis.using a microbroth method for aerobes and agar dilution for anaerobes, we studied the comparative in vitro activity of gemifloxacin, three fluoroquinolones, two macrolides and two beta-lactams, against 207 aerobic and 162 anaerobic antral sinus puncture isolates. gemifloxacin was active at < or = 0.25 ug/ml against 198/207 [96%] aerobes and 127/162 [78%] anaerobes or 325/369 [88%] of all isolates and was the most active quinolone on a weight basis against gram-positive organisms. all haemophilus ...200211858906
effects of instrumentation, irrigation and dressing with calcium hydroxide on infection in pulpless teeth with periapical bone lesions.the aim of this study was to evaluate the fate of microorganisms in root canals of teeth with infected pulps and periapical bone lesions with and without the use of calcium hydroxide medication.200211858203
[retrospective analysis of fusobacterium associated infections; experience at aomori prefectural hospital with 108 cases from 1995 to 1999].we experienced 108 cases of fusobacterium associated infections, including otolaryngeal, oral, pleuropulmonary, intraabdominal, skin and soft tissue infections, at aomori prefectural hospital during the 5 year-period from 1995 to 1999. a total of 433 organisms, included 113 fusobacterium spp. (80 fusobacterium nucleatum, 18 fusobaterium necrophorum, 5 fusobacterium varium, 4 fusobacterium mortiferum, 6 fusobacterium spp.), were recovered with an average of 4.0 organisms per case of the 108 cases ...200211852471
identification of predominant human and animal anaerobic intestinal bacterial species by terminal restriction fragment patterns (trfps): a rapid, pcr-based method.identification of predominant human and animal intestinal tract anaerobes by conventional methods is cumbersome, time-consuming and less sensitive as compared to molecular methods. we have developed a molecular technique to identify most of the abundant intestinal microflora by polyermase chain reaction (pcr) amplification of a 16s rrna gene fragment using a pair of universal pcr primers. the forward pcr primer was labelled with 6-carboxyfluorescein amino hexy (6-fam) fluorescent dye to detect t ...200111851378
antimicrobial effects of 2.94 microm er:yag laser radiation on root surfaces: an in vitro study.this in vitro study investigated the antimicrobial effects of 2.94 microm er:yag laser radiation on root surfaces.200211846852
in vitro antagonistic effect of lactobacillus on organisms associated with bacterial assess antagonistic properties of lactobacillus strains isolated from the vaginas of healthy women as compared to the most common bacterial agents related to vaginosis.200211838310
[anaerobic infection and its rapid detection in surgical patients].to investigate anaerobic infection and its rapid detection in surgical patients.199911829950
[a comparative study of various evaluation methods of the antibiotic sensitivity of strict anaerobic bacteria of the subgingival flora].the study on the sensitiveness of slow-growing anaerobes bacteria to antibiotics is delicate when you consider the technical motives that make it difficult to transpose the standard methods frequently used in microbiological laboratories. the three main methods used to determine susceptibility to antibiotics are: disk-diffusion test, antibiotics containing microdilution plates and atb ana (biomérieux). the aim of this study is to compare the effectiveness of each of these methods on severe anaer ...200111808376
periapical lesion progression with controlled microbial inoculation in a type i diabetic mouse model.uncontrolled or poorly controlled diabetes mellitus may be a risk factor for the development of large and/or debilitating periapical infections. the objectives of this investigation were to: (i) determine the effect of diabetes mellitus on the pathogenesis of periapical lesions with or without specific bacterial inoculations at the exposure sites, and (ii) test the sensitivities of two microbiological techniques in detecting the persistence of the bacterial inoculum in exposed pulps of nonobese ...200211806653
the microbiology of evaluate the aerobic and anaerobic microbiology of mucopyocele.200111801943
suppurative parotitis caused by anaerobic bacteria in newborns.staphylococci are the usual bacterial etiology of suppurative parotitis in newborns. this report describes for the first time recovery of anaerobic bacteria from aspirates of the infected gland in two infants with suppurative parotitis. peptostreptococcus intermedius and prevotella melaninogenica were isolated from one child and prevotella intermedia from the other patient. complete recovery occurred after 4 weeks of antimicrobial therapy.200211791111
accurate computer-based design of a new backbone conformation in the second turn of protein l.the rational design of loops and turns is a key step towards creating proteins with new functions. we used a computational design procedure to create new backbone conformations in the second turn of protein l. the protein data bank was searched for alternative turn conformations, and sequences optimal for these turns in the context of protein l were identified using a monte carlo search procedure and an energy function that favors close packing. two variants containing 12 and 14 mutations were f ...200211786026
ertapenem: a new carbapenem.ertapenem is a new 1-beta-methyl carbapenem, stable to dehydropeptidase, which binds preferable to penicillin-binding proteins (pbp) 2 and 3. ertapenem has a broad antibacterial spectrum with mic90 values < 0.5 mg/l for penicillin-susceptible streptococcus pneumoniae, streptococcus pyogenes, methicillin-sensitive staphylococcus aureus, haemophilus influenzae, moraxella catarrhalis, escherichia coli, citrobacter spp., klebsiella spp., serratia spp., proteus spp., clostridium perfringens, fusobact ...200111772242
[bacteria isolated from surgical infections and their susceptibilities to antimicrobial agents. special references to bacteria isolated between april 1998 and march 1999].the annual multicenter studies on isolated bacteria from infections in general surgery and their antimicrobial susceptibility have been conducted in japan since july 1982. this paper describes the results obtained in fiscal 1998 (from april 1998 to march 1999). the number of cases investigated as objectives was 225 for one year. a total of 429 strains (121 strains from primary infections and 308 strains from postoperative infections) were isolated from 183 cases (81.3% of total cases). in primar ...200111771334
the bacterial flora in inflammatory bowel disease: current insights in pathogenesis and the influence of antibiotics and probiotics.the pathogenesis of inflammatory bowel disease (ibd) remains unknown, although in recent years more data have become available. the contribution of genetic and environmental factors is evident, and the luminal bacterial flora plays a major role in the initiation and perpetuation of chronic ibd. animal models of ibd have shown that colitis does not occur in a germ-free environment. in human ibd, inflammation is present in parts of the gut containing the highest bacterial concentrations. moreover, ...200111768558
aggressive periodontitis associated with fanconi's anemia. a case report.fanconi's anemia is an autosomal recessive disease associated with chromosomal breakage as well as pancytopenia, skin pigmentation, renal hypoplasia, cardiac defects, microcephaly, congenital malformations of the skeleton, hypogonadism, and increased risk of leukemia. the present report describes the periodontal clinical and microbiological status of an 11-year old male having fanconi's anemia.200111759873
[use of the etest method for antimicrobial susceptibility testing of obligate anaerobes].the aim of this study was to evaluate etest usefulness for antimicrobial susceptibility testing of obligate anaerobes and to compare the activity of five antibacterial drugs against clinical strains of anaerobes. one hundred strains of obligate anaerobes were tested: 2 reference strains (b. fragilis atcc 25285 and b. thetaiotaomicron atcc 29741) and 98 clinical strains isolated from patients of the infant jesus clinical hospital--center for trauma treatment in warsaw during the last three years ...200111757426
structure, specificity, and mode of interaction for bacterial albumin-binding modules.we have determined the solution structure of an albumin binding domain of protein g, a surface protein of group c and g streptococci. we find that it folds into a left handed three-helix bundle similar to the albumin binding domain of protein pab from peptostreptococcus magnus. the two domains share 59% sequence identity, are thermally very stable, and bind to the same site on human serum albumin. the albumin binding site, the first determined for this structural motif known as the ga module, co ...200211751858
splenic abscess caused by peptostreptococcus species, diagnosed with the aid of abdominal computerized tomography and treated with percutaneous drainage and antibiotics: a case report.we describe herein a 63-year-old patient with a splenic abscess due to peptostreptococcus spp., diagnosed with the aid of abdominal computerised tomography and treated with ultra-sound guided percutaneous drainage and antibiotics. the bacteriological characteristics of splenic abscesses are discussed.200111744179
supragingival and subgingival microbiota of adult patients with down's syndrome. changes after periodontal this longitudinal study, five adult down's syndrome patients with periodontitis were placed on a frequent recall visit schedule (every 6 weeks) after treatment, in order to investigate: 1) the microbiological status, both supragingivally and subgingivally, and the changes that occurred after treatment and 2) the effect of frequent professional supragingival plaque control on the subgingival microbiota and clinical variables in these patients. the clinical variables recorded were probing pocke ...200111737662
association of early onset periodontitis microbiota with aspartate aminotransferase activity in gingival crevicular fluid.the objective of this study was to determine the relationship between the activity of the enzyme aspartate aminotransferase (ast) in gingival crevicular fluid (gcf) using the colorimetric periogard (ptm) test and the subgingival microflora in early onset periodontitis lesions.200111737506
[antimicrobial activities of carbapenems and fourth generation cephems against clinically isolated strains].we determined the antibacterial activities of 4 carbapenems (imipenem, panipenem, meropenem and biapenem) and 2 forth generation cephems (cefozopran and cefepime) against 850 bacterial strains (18 species) isolated during the period from january 1998 through september 2000. the 4 carbapenems showed excellent antibacterial activities against methicillin-susceptible s. aureus (mssa), s. pneumoniae, e. faecalis, p. aeruginosa, m. (b). catarrhalis, b. fragilis and peptostreptococcus spp. as compared ...200111729712
single-site mutations induce 3d domain swapping in the b1 domain of protein l from peptostreptococcus magnus.thermodynamic and kinetic studies of the protein l b1 domain (ppl) suggest a folding pathway in which, during the folding transition, the first beta hairpin is formed while the second beta hairpin and the alpha helix are largely unstructured. the same mutations in the two beta turns have opposite effects on the folding and unfolding rates. three of the four residues composing the second beta turn in ppl have consecutive positive phi angles, indicating strain in the second beta turn.200111709166
azotobacter vinelandii ferredoxin i: a sequence and structure comparison approach to alteration of [4fe-4s]2+/+ reduction potential.the reduction potential (e(0)') of the [4fe-4s](2+/+) cluster of azotobacter vinelandii ferredoxin i (avfdi) and related ferredoxins is approximately 200 mv more negative than the corresponding clusters of peptostreptococcus asaccharolyticus ferredoxin and related ferredoxins. previous studies have shown that these differences in e(0)' do not result from the presence or absence of negatively charged surface residues or in differences in the types of hydrophobic residues found close to the [4fe-4 ...200211704670
absence of a specific subgingival microflora in adults with down's syndrome.periodontal disease in down's syndrome (ds) is generally characterized by a high degree of bone loss. bone loss of 5 mm or more is observed in 70% of these subjects. among ds subjects, considerable differences in disease progression occur. so far, no studies have been conducted in which specific properties of the subgingival microflora have been related to the condition observed.200111686820
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