TitleAbstractYear(sorted descending)
bacterial penetration through canals of endodontically treated teeth in the presence or absence of the smear layer.the goal of this study is to determine if the smear layer affects the passage of bacteria through or around obturating material as evidenced by penetration of bacteria through and out the canal. specifically, this study focused on determining the effect of the smear layer on the magnitude of bacterial penetration through the apical foramen.200312735922
[utility of anaerobic blood cultures in extra-hospitalary bacteremias].to compare the rentability of anaerobic hemocultures with aerobic cultures in patients suffering bacteremia, trying to analyze the rentability of anaerobic cultures.200312768830
immunoglobulin-binding domains: protein l from peptostreptococcus magnus.protein l is a multidomain cell-wall protein isolated from peptostreptococcus magnus. it belongs to a group of proteins that contain repeated domains that are able to bind to igs without stimulating an immune response, the most characterized of this group being protein a ( staphylococcus aureus ) and protein g ( streptococcus ). both of these proteins bind predominantly to the interface of c(h)2-c(h)3 heavy chains, while protein l binds exclusively to the v(l) domain of the kappa -chain. the fun ...200312773190
identification of oral peptostreptococcus isolates by pcr-restriction fragment length polymorphism analysis of 16s rrna genes.oral peptostreptococcus isolates tentatively identified by conventional microbiological culture methods were identified to the species level by a combination of pcr amplification of 16s rrna genes and restriction enzyme analysis of the amplified products. this method is a reliable and rapid alternative to conventional methods for identification of these bacterial species.200312958299
[early diagnosis of bacterial brain abscesses: interest of diffusion-weighted mri].three cases of bacterial brain abscesses, in immunocompetent patients, are reported. in all these cases, the diffusion-weighted magnetic resonance (mri) with apparent diffusion coefficient (adc) map has permitted an early diagnosis and a rapid treatment. this emergency mri showed in the three cases a low signal on ti-weighted images, a high signal on t2-weighted and echo-planar images, and a decrease of adc (0.36- 0.49 x 10(-3) mm2/s). so, this new mri technique provides an available and rapid e ...200312773871
deep neck infection: a present-day complication. a retrospective review of 83 cases (1998-2001).deep neck infections are less and less frequent today than in the past. nevertheless, their complications are often life-threatening. the present study reviews the experience of the department of otolaryngology and head and neck surgery of padua with deep neck infections during the period from 1998 to 2001. eighty-three patients (55 males and 28 females) were retrospectively considered. the site of origin of deep neck infection was identified in 76 patients (91%). the most common cause was denta ...200312774234
[modifying action of oxytocin on the biological properties of the causative agents of anaerobic non-clostridial infection].in a number of in vitro experiments the effect of oxytocin on the antilysozyme and anticomplemental activity of propiobacterium propionicum, bacteroides fragilis, prevotella melaninogenica and peptostreprtococcus anaerobius, isolated from patients with acute pyoinflammatory pleuropulmonary diseases, was studied. antibiotic resistance dynamics of the infective agents under study to lincomycin, clindamycin, thienam, vancomycin was also detected. the inhibiting activity of oxytocin on the persisten ...200312966884
late infection in patients with scoliosis treated with spinal instrumentation.sixty patients were treated using a multilevel spinal instrumentation system. spine arthrodesis was done posteriorly in all patients using a combination of two rods, hooks, screws, and cross-link plates. the galveston technique was used in three patients. five patients presented with late deep wound infections 1 to 5 years postoperatively. two patients presented with a local subcutaneous abscess, whereas the remaining patients had a local drainage. exploration revealed pus lining the instrumenta ...200312782866
peptostreptococcus micros in primary endodontic infections as detected by 16s rdna-based polymerase chain reaction.a 16s rdna-based polymerase chain reaction (pcr) method was used to detect peptostreptococcus micros in primary root canal infections. samples were collected from 50 teeth having carious lesions, necrotic pulps, and different forms of periradicular diseases. dna extracted from the samples was amplified using the pcr assay, which yielded a specific fragment of p. micros 16s rdna. p. micros was detected in 6 of 22 root canals associated with asymptomatic chronic periradicular lesions (27.3%), 2 of ...200312597709
activities of garenoxacin (bms-284756) and other agents against anaerobic clinical isolates.a total of 590 clinical isolates consisting of 33 species of both gram-positive and gram-negative anaerobes were collected from nine centers in the chicago area in 1998-1999. the largest number of isolates (330 isolates, 56%) belonged to the bacteroides group. isolates were tested by agar dilution against garenoxacin (bms-284756, t-3811 me), trovafloxacin, moxifloxacin, clindamycin, imipenem, piperacillin-tazobactam, and cefoxitin. all but one species (2% of bacteroides vulgatus isolates) were f ...200312604521
the effects of a triclosan/copolymer dentifrice on oral bacteria including those producing hydrogen sulfide.a clinical procedure was developed to examine the effects of short-term and extended use of a triclosan/copolymer dentifrice and a commercial fluoride dentifrice on oral bacteria, including those producing hydrogen sulfide. healthy adults volunteered for this double-blind, crossover design clinical study and provided saliva samples for culturing on enriched and indicator media to enumerate all salivary bacteria and those producing hydrogen sulfide (odorigenic), respectively. subjects brushed wit ...200312786953
bacteriological study of root canals associated with periapical abscesses.the aim of this study was to identify microorganisms from root canals with periapical abscesses and to ascertain the susceptibility of peptostreptococcus prevotii and fusobacterium necrophorum to antimicrobials. study design thirty root canals were microbiologically sampled by using sterile paper points. the concomitant microorganisms were identified through the use of established methods. the susceptibility of p prevotii and f necrophorum to antimicrobials was evaluated by using the e test meth ...200312973290
endocarditis due to peptostreptococcus anaerobius: case report and literature review of peptostreptococcal endocarditis.infective endocarditis caused by anaerobic streptococci occurs rarely, and only five cases have been described in detail, with two additional cases mentioned in a review of anaerobic infective arthritis. all have resulted from infection caused by peptostreptococci. another case of anaerobic gram-positive endocarditis caused by peptostreptococcus anaerobius has recently been encountered. the patient was successfully treated with a beta-lactam for six weeks, plus gentamicin (to which the organism ...200312803344
effect of smoking and periodontal treatment on the subgingival microflora.the effect of smoking on the prevalence of periodontal pathogens after periodontal treatment is still not clear. some studies found no effect of the smoking status on the prevalence of periodontal pathogens after therapy, whereas others did. the aim of this retrospective study was to investigate the influence of smoking on the treatment of periodontitis and the composition of the subgingival microflora.200312834497
comparative in vitro activity of ph-027 versus linezolid and other anti-anaerobic antimicrobials against clinical isolates of clostridium difficile and other anaerobic is a new 5-triazole oxazolidinone synthesized in our laboratories, which shows strong activity against gram-positive aerobic bacteria including clinical isolates. the objective of this study was to investigate the in vitro activity of this compound in comparison with linezolid and other antibiotics against gram-positive and gram-negative anaerobes. the in vitro activity of ph-027 in comparison with those of linezolid and other antimicrobial agents was evaluated against 201 clinical isolat ...200312797385
antibacterial activity of medicinal plant extracts against periodontopathic bacteria.this study was performed to evaluate the antibacterial activity of althaea officinalis l. roots, arnica montana l. flowers, calendula officinalis l. flowers, hamamelis virginiana l. leaves, illicium verum hook. fruits and melissa officinalis l. leaves, against anaerobic and facultative aerobic periodontal bacteria: porphyromonas gingivalis, prevotella spp., fusobacterium nucleatum, capnocytophaga gingivalis, veilonella parvula, eikenella corrodens, peptostreptococcus micros and actinomyces odont ...200312820224
in vitro activities of ramoplanin, teicoplanin, vancomycin, linezolid, bacitracin, and four other antimicrobials against intestinal anaerobic using an agar dilution method, the in vitro activities of ramoplanin, teicoplanin, vancomycin, linezolid, and five other agents were determined against 300 gram-positive and 54 gram-negative strains of intestinal anaerobes. ramoplanin was active at <or=2 microg/ml against 287 of 300 (95.7%) gram-positive organisms, including 18 strains of clostridium difficile for which mics of ramoplanin were 0.25 to 0.5 microg/ml; for 3 of these, linezolid mics were 8 to 16 micro g/ml. nineteen clostridium ...200312821492
microorganisms from canals of root-filled teeth with periapical lesions.the objective of the present study was to identify the microbial flora within root canals of teeth with failed root-canal treatment and to determine the association of the various species with clinical features.200312656508
an immunohistological study of the localization of bacteria invading root pulpal walls of teeth with periapical lesions.we immunohistologically examined the prevalence and localization of bacteria invading dentinal tubules of the roots of teeth with infected canals. forty extracted teeth with apical lesions were selected and divided into two groups: a group of untreated teeth and a group of canal-enlarged teeth. the bacteria in the specimens were detected by brown-brenn stain and the labeled-streptavidin-biotin method with specific antisera for 16-bacteria. seventy percent of the examined teeth showed bacteria in ...200312669880
antimicrobial properties of self-etching primer-bonding systems.self-etching primers are now considered the new generation of dentin bonding systems that modify and incorporate the bacteria-containing smear layer into their bonding mechanism. the antibacterial effects of the self-etching primers clearfil se bond, mac bond, imperva fl bond, one-up bondf and prompt l-pop were evaluated using the bacteria streptococcus mutans atcc25175, peptostreptococcus anaerobius, peptostreptococcus prevotii, peptostreptococcus asaccharolyticus, lactobacillus acidophilus, la ...200312670069
transformation of arctiin to estrogenic and antiestrogenic substances by human intestinal bacteria.after anaerobic incubation of arctiin (1) from the seeds of arctium lappa with a human fecal suspension, six metabolites were formed, and their structures were identified as (-)-arctigenin (2), (2r,3r)-2-(3',4'-dihydroxybenzyl)-3-(3",4"-dimethoxybenzyl)butyrolactone (3), (2r,3r)-2-(3'-hydroxybenzyl)-3-(3",4"-dimethoxybenzyl)butyrolactone (4), (2r,3r)-2-(3'-hydroxybenzyl)-3-(3"-hydroxy-4"-methoxybenzyl)butyrolactone (5), (2r,3r)-2-(3'-hydroxybenzyl)-3-(3",4"-dihydroxybenzyl)butyrolactone (6), and ...200312672988
evaluation of root canal microorganisms isolated from teeth with endodontic failure and their antimicrobial susceptibility.studies of the microbiota from the canals of teeth with failure of endodontic therapy have revealed that it differs markedly from that of untreated necrotic dental pulps. this study aimed to evaluate the microbiota of 30 root-filled teeth with persisting periapical lesions and to test the antibiotic susceptibility of the most prevalent species. microbial samples, isolation and speciation were done using advanced microbiologic techniques for anaerobic species. a total of 55 bacterial species were ...200312654099
differences in antimicrobial activity of four commercial 0.12% chlorhexidine mouthrinse formulations: an in vitro contact test and salivary bacterial counts evaluate the in vitro and in vivo antimicrobial activity of four commercial 0.12% chlorhexidine mouthrinses.200312694428
dna from periodontopathogenic bacteria is immunostimulatory for mouse and human immune cells.although bacterial dna (bdna) containing unmethylated cpg motifs stimulates innate immune cells through toll-like receptor 9 (tlr-9), its precise role in the pathophysiology of diseases is still equivocal. here we examined the immunostimulatory effects of dna extracted from periodontopathogenic bacteria. a major role in the etiology of periodontal diseases has been attributed to actinobacillus actinomycetemcomitans, porphyromonas gingivalis, and peptostreptococcus micros. we therefore isolated d ...200312540566
in vitro activities of telithromycin and 10 oral agents against aerobic and anaerobic pathogens isolated from antral puncture specimens from patients with sinusitis.a study of the comparative in vitro activity of telithromycin, a new ketolide, against 155 aerobic and 171 anaerobic antral sinus puncture isolates showed it to be active against a broad range of sinus pathogens. all pneumococci, including erythromycin-resistant strains, were susceptible to telithromycin at < or = 0.5 microg/ml; all haemophilus influenzae and eikenella corrodens strains were inhibited by < or = 4 microg of telithromycin/ml; all moraxella spp. and beta-lactamase-producing prevote ...200312760875
in vitro activities of dalbavancin and nine comparator agents against anaerobic gram-positive species and corynebacteria.dalbavancin is a novel semisynthetic glycopeptide with enhanced activity against gram-positive species. its comparative in vitro activities and those of nine comparator agents, including daptomycin, vancomycin, linezolid, and quinupristin-dalfopristin, against 290 recent gram-positive clinical isolates strains, as determined by the nccls agar dilution method, were studied. the mics of dalbavancin at which 90% of various isolates tested were inhibited were as follows: actinomyces spp., 0.5 microg ...200312760876
acute bacterial suppurative parotitis: microbiology and management.the parotid gland is the salivary gland most commonly affected by inflammation. the most common pathogens associated with acute bacterial parotitis are staphylococcus aureus and anaerobic bacteria. the predominant anaerobes include gram-negative bacilli (including pigmented prevotella and porphyromonas spp.), fusobacterium spp., and peptostreptococcus spp. streptococcus spp. (including s. pneumoniae) and gram-negative bacilli (including escherichia coli) have also been reported. gram-negative or ...200312544218
the microflora recovered from the outer-surfaces of the frialit-2 implanto-prosthetic connector.the aim of the present investigation was to examine the outer-surface microbiota of the prosthetic connector of frialit-2 implants, and to compare the microbial findings with the peri-implant parameters 2 years after functional loading. in 16 implant-treated patients (55.8 +/- 9.5 years) the outer-surface micro-organisms of the prosthetic connectors were determined in 32 frialit-2 implants. the functional loading time of the prosthetic suprastructures was 24.1 +/- 13.8 months on average. after r ...200312562362
in vitro and in vivo antibacterial activities of telithromycin.telithromycin is one of the ketolides, characterised by a 3-keto group instead of l-cladinose and a c(11)-c(12) carbamate link by an alkyl chain to a pyridinum and imidazolium ring side chain. we evaluated in vitro and in vivo antibacterial activities of telithromycin against gynaecological pathogens.200312714813
comparison of ligand polarization and enzyme activation in medium- and short-chain acyl-coenzyme a dehydrogenase-novel analog complexes.spectroelectrochemical and off-resonance raman indicate that substrate/product binding to medium-chain acyl-coenzyme a (coa) dehydrogenase (pmcad) results in ligand polarization and positive flavin potential shifts, which activate the enzyme for electron transfer. bacterial short-chain acyl-coa dehydrogenase (bscad) typically exhibits smaller potential shifts upon substrate/product binding that have not been linked to ligand polarization. to further investigate the roles of ligand binding and po ...200312504892
immunoglobulin superantigen protein l induces il-4 and il-13 secretion from human fc epsilon ri+ cells through interaction with the kappa light chains of ige.peptostreptococcus magnus protein l is a multidomain bacterial surface protein that correlates with virulence. it consists of up to five homologous ig-binding domains (b1-b5) that interact with the variable domain of ig kappa l chains. intact protein l stimulates the synthesis and the release of il-4 and il-13 from human basophils in vitro. a protein l fragment covering the ig-binding domains b1-b4 also induced il-4 and il-13 release from basophils. there was an excellent correlation (r(s) = 0.8 ...200312574351
additive antimicrobial activity of calcium hydroxide and chlorhexidine on common endodontic bacterial is an established procedure to use calcium hydroxide [ca(oh)2] as a therapeutic component to achieve sterilization of infected root canals. unfortunately, some bacterial species are relatively resistant to ca(oh)2 and are therefore associated with treatment failures. the objective of this study was to identify combinations of substances that improve antimicrobial activity. ca(oh)2 was used as a suspension or as a component of gutta-percha points. efficacy of killing was compared for (a) ca(oh ...200312775007
fusobacterial brain abscess: a review of five cases and an analysis of possible pathogenesis.the cases of five patients with fusobacterial brain abscess are presented. the authors discuss their attempt to determine the pathogenesis.200314567605
bacterial artificial chromosome library of finegoldia magna atcc 29328 for genetic mapping and comparative genomics.we constructed a bacterial artificial chromosome (bac) library of finegoldia magna atcc 29328 dna to facilitate further genome analysis of f. magna. the bac library contained 385 clones with an average insert size of 55 kb, representing a 10.1-fold genomic coverage. repeated dna hybridization using primer sets designed on the basis of bac-end sequences yielded nine contigs covering 95% of the chromosome and two contigs covering 98% of the plasmid. the contigs were localized on the physical map o ...200314695451
inflammatory polyp in the middle ear with secondary suppurative meningoencephalitis in a cat.a 15-month-old male maine coon cat presented with persistent auricular discharge and progressive head tilt, ataxia, and loss of blink on the right side. using computed tomography a hyperattenuating, contrast-enhancing material within a thickened right tympanic bulla and contrast enhancement of the adjacent cerebellum were identified. marked suppurative inflammation was identified on cerebrospinal fluid analysis with no growth on bacterial culture. ventral bulla osteotomy was performed to remove ...200314703245
normal ocular features, conjunctival microflora and intraocular pressure in the canadian beaver (castor canadensis).the aim of the study was to assess the ocular features, normal conjunctival bacterial and fungal flora, and intraocular pressure (iop) in the canadian beaver (castor canadensis).200314641823
la, ptb, and pab proteins bind to the 3(') untranslated region of norwalk virus genomic rna.noroviruses are human enteric caliciviruses for which no cell culture is available. consequently, the mechanisms and factors involved in their replication have been difficult to study. in an attempt to analyze the cis- and trans-acting factors that could have a role in nv replication, the 3(')-untranslated region of the genome was studied. use of zuker's mfold-2 software predicted that nv 3(')utr contains a stem-loop structure of 47 nts. proteins from hela cell extracts, such as la and ptb, form ...200314623338
clinical presentation and bacteriologic analysis of infected human bites in patients presenting to emergency departments.previous studies of infected human bites have been limited by small numbers of patients and suboptimal microbiologic methodology. we conducted a multicenter prospective study of 50 patients with infected human bites. seventy percent of the patients and assailants were young adult men. fifty-six percent of injuries were clenched-fist injuries and 44% were occlusional bites. most injuries were to the hands. fifty-four percent of patients were hospitalized. the median number of isolates per wound c ...200314614671
planning of empirical antibiotic therapy for women with pelvic inflammatory diseases: a geographical area-specific study.elaboration of an empiric antibiotic regimen for women with pelvic inflammatory disease (pid) for a geographical area in eastern hungary.200314597249
importance of sequence specificity for predicting protein folding pathways: perturbed gaussian chain model.recent experimental and theoretical studies suggest that rates and pathways of protein folding are largely decided by topology of the native structures, at least for small proteins. however, some exceptions are known; for example, protein l and protein g have the same topology, but exhibit different characteristics of the tse. thus, folding pathways of some proteins are critically affected by detailed information on amino acid sequences. to investigate the sequence specificity, we calculate fold ...200314579353
microbial dynamics of purulent nasopharyngitis in children.this review presents the microbiological dynamic and therapeutic options in the management of purulent nasopharyngitis (npt). the nasopharynx (np) of healthy children is generally colonized by relatively non-pathogenic aerobic and anaerobic organisms, some of, which possess the ability to interfere with the growth of potential pathogens. conversely, carriage of potential respiratory aerobic pathogen such as streptococcus pneumoniae, haemophilus influenzae and moraxella catarrhalis, as well as so ...200314550957
ct of a ruptured pyomyoma. 200314573437
[anaerobic bacteria in bronchoalveolar lavage fluid (bal) after thoracic surgery].purpose of this study was to find out what kind of anaerobic bacteria were in lower respiratory tract and how often they were present there considering patients after thoracic surgery. also, what is susceptibility of bacteria to antibiotics. research covered 30 patients after operation. material for research was bronchoalveolar lavage (bal) taken during bronchoscopy. collected sample was cultivated in anaerobic and aerobic conditions. anaerobic bacteria were found in 28 samples (93%). totally th ...200314577197
distribution of periodontal pathogens in korean aggressive periodontitis.microbial associations in aggressive periodontitis versus different ethnic origins are substantially unknown. we undertook this study to determine the prevalence of seven putative periodontopathogens in korean patients and to evaluate microbial differences in localized and generalized aggressive periodontitis patients.200314584866
infection-associated hemophagocytic syndrome in a diabetic patient undergoing chronic hemodialysis.hemophagocytic syndrome (hps) is an uncommon but severe illness associated with a variety of infections, malignant tumors, and autoimmune diseases. we report a case of infection-associated hps in a patient receiving chronic hemodialysis. peptostreptococcus-induced sepsis and abscess formation in the left iliopsoas muscle led to the onset of infection-associated hps in this patient. the patient had diabetes mellitus and end-stage renal disease, and it is likely that immunological dysfunctions fro ...200314586736
[the presence of bacteria and yeast like fungi in specimens from surgical patients].the aim of this study was to determine the incidence of candida spp. strains in specimens obtained from surgically treated patients as well as to analyze the accompanying bacterial flora, both aerobic and anaerobic. the material came from two groups of patients. in the first group consisting of patients operated for colon and rectum carcinoma, the samples included peritoneal fluid, colon or rectum bioptates, pus, blood, and wound swabs. in the other group, biopsy material and smears from post op ...200314577199
[antibacterial activity of oral cephems against various clinically isolated strains].we determined the antibacterial activities of oral cephems against isolated from the patients with the respiratory infections, the urinary tract infections, and infections in the obstetrics field of an adult and a child, during the period from 2002 to 2003; streptococcus pyogenes, streptococcus pneumoniae, haemophilus influenzae, branhamella catarrhalis, klebsiella pneumoniae and escherichia coli of 40 strains of each, and peptostreptococcus spp. 22 strains. s. pneumoniae and h. influenzae strai ...200315007876
characteristics of anaerobes from skin specimens.anaerobes isolated from skin specimens from 1999 to 2001 were examined. the most common type was peptostreptococcus spp., especially p. magnus and p. assaccharolyticus and bacteroides fragilis. dominance was seen for p. magnus, p. acnes and p. prevotii. peptostreptococcus spp. and p. acnes showed high susceptibility to four antimicrobial agents. prevotella spp. and b. fragilis showed low or no susceptibility to ampicillin, while b. fragilis showed low susceptibility to ceftizoxime. evaluation of ...200315018306
role of anaerobes in acute pelvic inflammatory disease.pouch of douglas aspirates were collected from 50 women with history and examination suggestive of acute pelvic inflammatory disease (pid) and 20 healthy women admitted for tubal ligation served as control. a total of 57 microorganisms were isolated from 37 patients out of 50 in study group. of 37 positive cultures 21(56.7%) were monomicrobial and 16(43.2%) were polymicrobial. most common symptom in study group was lower abdominal pain (90%), vaginal discharge (70%) and irregular bleeding (40%) ...200317643017
anaerobic bacteria cultured from the tongue dorsum of subjects with oral malodor.the bacteria on the dorsum of the tongue are the most frequent cause of oral malodor; however, the bacterial flora of the tongue has not been well defined. although recent studies have used dna probes to detect the presence of certain periodontal pathogens, cultural studies have been limited because of the complexity of the flora of the tongue dorsum. the purpose of this study was to grow and to identify maximum numbers of capnophylic gram-negative bacilli and anaerobic micro-organisms by cultur ...200316887710
e-test antibiotics susceptibility of strict anaerobic bacteria.the e-test is convenient for testing susceptibility of anaerobes. from september 1998 to september 1999, 194 strains (105 gram-positive bacteria, 89 gram-negative bacteria) of clinically relevant samples were tested against five antibiotics benzylpenicillin, amoxicillin-clavulanic acid, clindamycin, metronidazole and imipenem on blood agar plates. resistance to benzyl penicillin is widespread and gram-negative bacteria and resistance to amoxicillin-clavulanic acid is exceptional. metronidazole i ...200316887712
an in vitro time-kill assessment of linezolid and anaerobic bacteria.linezolid is a novel oxazolidinone antibacterial agent active against staphylococci (including methicillin-resistant strains), enterococci (including vancomycin-resistant strains), streptococci (including penicillin-intermediate and -resistant streptococcus pneumoniae), and other aerobic and facultative bacteria. the agent has also demonstrated activity against a broad spectrum of gram-positive and gram-negative anaerobic bacteria. previous time-kill assessments have shown linezolid to be genera ...200316887680
antibiotic susceptibility of clinically relevant anaerobes in estonia from 1999 to present little or no data is available regarding the resistance profiles of anaerobic bacteria in relation to the general usage of antibiotics. the objective of this study was to assess whether any potential relationship exists between the dynamics of antibiotic resistance of anaerobic bacteria and the consumption of antibiotics during the last 3 years within the estonian population. in total, 416 anaerobic isolates were investigated from various clinical samples. the anaerobes were isolated ...200316887688
microbial flora in orodental infections.the present study was carried out to compare the normal aerobic and anaerobic bacterial oral flora with flora from deep seated dental caries, gingivitis and adult periodontitis. all the samples belonging to both the control and study groups yielded microbes. aerobe / anaerobe ratio was high in normal flora (1.48) as compared to dental caries (0.9), gingivitis (0.72) and periodontitis (0.56). ninety seven percent of orodental infections were polymicrobial and three or more microbes were found in ...200317642993
anaerobes in bacterial vaginosis.four hundred high vaginal swabs were taken from patients attending gynaecology and obstetrics department of govt. medical college, amritsar. the patients were divided into four groups i.e. women in pregnancy (group i), in labour/post partum (group ii), with abnormal vaginal discharge or bacterial vaginosis (group iii) and asymptomatic women as control (group iv). anaerobic culture of vaginal swabs revealed that out of 400 cases, 212(53%) were culture positive. maximum isolation of anaerobes was ...200317642997
actinomycotic bacteraemia after dental assess role of oral flora in causing bacteraemia following dental manipulations.200217657035
anaerobes in pleuropulmonary infections.a total of 76 anaerobes and 122 aerobes were isolated from 100 patients with pleuropulmonary infections, e.g. empyema (64), pleural effusion (19) and lung abscess (13). in 14% of the patients, only anaerobes were recovered, while a mixture of aerobes and anaerobes was encountered in 58%. from all cases of lung abscess, anaerobic bacteria were isolated, alone (04) or along with aerobic bacteria (13). from empyema and pleural effusion cases, 65.6% and 68.4% anaerobes were recovered respectively. a ...200217657055
taxonomy--general comments and update on taxonomy of clostridia and anaerobic cocci.since such things as sites of normal carriage, type and severity of infections produced, and antimicrobial susceptibility patterns may vary according to the particular species involved, accurate taxonomy for differentiation of species is important. for example, reclassification of the 'bacteroides gracilis group' revealed that sutterella wadsworthensis is actually responsible for the antimicrobial resistance seen in that group. with clostridia and anaerobic cocci, as with other taxa, there have ...200216887670
bacterial diversity in necrotizing ulcerative periodontitis in hiv-positive subjects.necrotizing ulcerative periodontitis (nup) is a painful and potentially debilitating affliction that affects about 2% to 6% of hiv-positive subjects. nup may be caused by specific microorganisms that are presently unknown or by microbial species not usually thought to cause periodontal infections. the purpose of this study was to define the bacterial species associated with nup in hiv-positive patients.200216013212
[the microorganisms associated with bacterial vaginosis as a cause of tubal sterility].bacterial vaginosis is one of the most widespread disturbances of the normal state of the vagina of women of reproductive and menopausal age. this condition is highly disregarded due to its moderate and not always clear clinical manifestation. the disturbed proportion of microorganisms in vagina is shown in the strong decrease or lack of lactobacilli and a spread of associated with bacterial vaginosis microorganisms. g. vaginalis, mobiluncis spp., bacteroides spp. (without b. fragilis), peptostr ...200212516260
management of complex multi-space odontogenic infections.the successful management of multi-space orofacial odontogenic infections involves identification of the source of the infection, the anatomical spaces encountered, the predominant microorganisms that are found during the various stages of odontogenic fascial space infection, the impact of the infectious process on defense systems, the ability to use and interpret laboratory data and imaging studies, and a thorough understanding of contemporary antibiotic and supportive care. the therapeutic goa ...200212572406
comparison of 16s rdna-based pcr and checkerboard dna-dna hybridisation for detection of selected endodontic pathogens.molecular methods have been used recently to investigate the bacteria encountered in human endodontic infections. the aim of the present study was to compare the ability of a 16s rdna-based pcr assay and checkerboard dna-dna hybridisation in detecting actinobacillus actinomycetemcomitans, bacteroides forsythus, peptostreptococcus micros, porphyromonas endodontalis, por. gingivalis and treponema denticola directly from clinical samples. specimens were obtained from 50 cases of endodontic infectio ...200212466407
metabolism of pyrrolizidine alkaloids by peptostreptococcus heliotrinreducens and a mixed culture derived from ovine ruminal fluid.a mixed culture of ovine ruminal microbes metabolizes the macrocyclic pyrrolizidine alkaloids present in the plant senecio jacobaea, including jacobine and seneciphylline. previous attempts to identify metabolites of these alkaloids have not been successful. the objective of this study was to compare the metabolism of pyrrolizidine alkaloids by a mixed culture of ovine ruminal microbes to the metabolism of pyrrolizidine alkaloids by the known organism peptostreptococcus heliotrinreducens. p. hel ...200212488016
[identification and susceptibility to antimicrobial agents of strictly anaerobic bacteria isolated from hospitalized patients].the aim of this study was to identify anaerobic strains isolated in 2001 from clinical specimens obtained from patients of warsaw hospital and to evaluate a susceptibility of these strains to antimicrobial agents. in 2001 two hundred and twenty five clinical strains of obligate anaerobes were cultured, which were identified in the automatic atb system (biomérieux, france) using biochemical tests api 20 a. drug-susceptibility of strains was determined also in atb system with the use of atb ana st ...200212632658
porphyromonas gingivalis, bacteroides forsythus and other putative periodontal pathogens in subjects with and without periodontal destruction.bacteria play an essential role in the pathogenesis of destructive periodontal disease. it has been suggested that not all bacteria associated with periodontitis may be normal inhabitants of a periodontally healthy dentition. in particular, porphyromonas gingivalis and actinobacillus actinomycetemcomitans have been isolated infrequently from subjects without periodontitis. the aim of the present study was to compare prevalence and proportions of a number of periodontal bacteria in periodontitis ...200212472995
antibacterial activity of moxifloxacin against periodontal anaerobic pathogens involved in systemic infections.the in vitro activity of moxifloxacin was compared with that of penicillin g, amoxycillin/clavulanate, cefoxitin, erythromycin, clindamycin and metronidazole against 158 isolates associated with periodontal infections. mic(50)/mic(90) values of moxifloxacin were respectively 0.06/0.5 mg/l for porphyromonas gingivalis (35), for prevotella spp. (28) and actinomyces spp. (35), 0.12/0.25 mg/l for fusobacterium nucleatum (20) and 0.06/0.12 mg/l for peptostreptococcus spp. (30). the minimum inhibitory ...200212458140
evidence for plasticity and structural mimicry at the immunoglobulin light chain-protein l interface.the multidomain bacterial surface protein l (ppl) is a virulence factor expressed by only 10% of peptostreptococcus magnus strains, and its expression is correlated with bacterial vaginosis. the molecular basis for its ability to recognize 60% of mammalian immunoglobulin light chain variable regions (v(l)) has been described recently by x-ray crystallography, which suggested the presence of two v(l) binding sites on each protein l domain (graille, m., stura, e. a., housden, n. g., beckingham, j. ...200212221088
antimicrobial susceptibility of major pathogens of orofacial odontogenic infections to 11 beta-lactam this study, we evaluated the current effectiveness of 11 beta-lactam antibiotics for treatment of orofacial odontogenic infections by determining the antimicrobial susceptibility of the major pathogens. the antimicrobial susceptibilities of viridans streptococci (n = 47), peptostreptococcus (n = 67), porphyromonas (n = 18), fusobacterium (n = 57), black-pigmented prevotella (n = 59) and non-pigmented prevotella (n = 47) isolated from pus specimens of 93 orofacial odontogenic infections to pen ...200212354209
deuterium spin probes of side-chain dynamics in proteins. 2. spectral density mapping and identification of nanosecond time-scale side-chain the previous paper in this issue we have demonstrated that it is possible to measure the five different relaxation rates of a deuteron in (13)ch(2)d methyl groups of (13)c-labeled, fractionally deuterated proteins. the extensive set of data acquired in these experiments provides an opportunity to investigate side-chain dynamics in proteins at a level of detail that heretofore was not possible. the data, acquired on the b1 domain of peptostreptococcal protein l, include 16 (9) relaxation measu ...200212033876
vaginal bacterial flora activates rat peritoneal mast cells.sixteen strains of physiological and pathological vaginal bacteria were tested for their ability to secrete histamine from rat peritoneal mast cells in vitro. we noticed that mycoplasma hominis-induced histamine release was very high (up to 53.6%). the stimulation of rat mast cells with staphylococccus cohnii, staphylococcus coagulase(-) (two strains), ureaplasma urealyticum, peptostreptococcus spp., bacteroides capillosus, staphylococcus aureus and streptococcus agalactiae resulted in lower but ...200212575924
[a throat infection with rapid deterioration]. 200212362714
initial effect of controlled release chlorhexidine on subgingival microorganisms.little or no data exist on the ability of subgingival application of periochip (2.5 mg chlorhexidine gluconate in a biodegradable chip; astra pharmaceuticals, westborough, ma, usa) to suppress periodontopathic microorganisms. the present study compared the subgingival microbiota of periodontitis sites receiving the chlorhexidine chip plus scaling and root planing (sc/rp) or sc/rp alone.200212366861
sequence variations within protein families are linearly related to structural is commonly believed that similarities between the sequences of two proteins infer similarities between their structures. sequence alignments reliably recognize pairs of protein of similar structures provided that the percentage sequence identity between their two sequences is sufficiently high. this distinction, however, is statistically less reliable when the percentage sequence identity is lower than 30% and little is known then about the detailed relationship between the two measures of s ...200212381308
recovery of anaerobic bacteria from a glossal abscess in an adolescent.glossal abscess is infrequent in children. anaerobic bacteria are rarely recovered from this infection and never have been reported in children or adolescents. a 15-year-old patient presented with a tongue abscess following trauma. aspirate of the abscess yielded polymicrobial anaerobic flora: prevotella melaninogenica, fusobacterium nucleatum, and peptostreptococcus micros. the patient recovered following incision and drainage and 14 days of antimicrobial therapy with clindamycin. this report i ...200212395007
joint and bone infections due to anaerobic bacteria in children.the current review describes the microbiology, diagnosis and management of septic arthritis and osteomyelitis due to anaerobic bacteria in children. staphylococcus aureus, haemophilus influenzae type-b, and group a streptococcus, streptococcus pneumoniae, kingela kingae, neisseria meningiditis and salmonella spp are the predominant aerobic bacteria that cause arthritis in children. gonococcal arthritis can occur in sexually active adolescents. the predominant aerobes causing osteomyelitis in chi ...200212396847
phenolic antibacterials from piper betle in the prevention of halitosis.piper betle l. (piperaceae) leaves which are traditionally used in india and china in the prevention of oral malodor was examined by bioassay-guided fractionation to yield allylpyrocatechol (apc) as the major active principle which showed promising activity against obligate oral anaerobes responsible for halitosis. the biological studies with apc indicated that the potential to reduce methylmercaptan and hydrogen sulfide was mainly due to the anti-microbial activity as established using dynamic ...200212413722
two-part implants inserted in a one-stage or a two-stage procedure. a prospective comparative study.the aim of this study was to evaluate the feasibility of using a two-part implant system in a one-stage procedure and to monitor the microflora in the peri-implant area in relation to clinical and radiographic outcome.200212445222
[bacteria isolated from surgical infections and their susceptibilities to antimicrobial agents--special references to bacteria isolated between april 2000 and march 2001].tendency of isolated bacteria from infections in general surgery and their antimicrobial susceptibilities during the period from april 2000 to march 2001 were investigated in a multicenter study in japan, and the following results were obtained. the number of cases investigated as objectives was 234 for one year. a total of 388 strains (136 strains from primary infections and 252 strains from postoperative infections) were isolated from 165 cases (70.5% of total cases). in primary infections, an ...200212621729
heterogeneity within the gram-positive anaerobic cocci demonstrated by analysis of 16s-23s intergenic ribosomal rna polymorphisms.peptostreptococci are gram-positive, strictly anaerobic bacteria which, although regarded as members of the commensal human microflora, are also frequently isolated from sites of clinical infection. the study of this diverse group of opportunist pathogens has been hindered by an inadequate taxonomy and the lack of a valid identification scheme. recent re-classification of the peptostreptococcus family into five distinct genus groups has helped to clarify the situation. however, this has been on ...200212448679
bacteriology of acute and chronic sphenoid sinusitis.aspirates of 16 acutely infected and 7 chronically infected sphenoid sinuses were processed for aerobic and anaerobic bacteria. a total of 29 isolates were recovered from the 16 cases of acute sphenoid sinusitis (1.8 per specimen): 22 aerobic and facultative (1.4 per specimen), and 7 anaerobic (0.4 per specimen). aerobic and facultative organisms alone were recovered in 10 specimens (62%), anaerobes alone were isolated in 3 (19%), and mixed aerobic and anaerobic bacteria were recovered in 3 (19% ...200212450174
[bacteria isolated from surgical infections and their susceptibilities to antimicrobial agents. special references to bacteria isolated between april 1999 and march 2000].the annual multicenter studies on isolated bacteria from infections in general surgery and their antimicrobial susceptibility have been conducted in japan since july 1982. in this paper, the results obtained in the academic year 1999 (from april 1999 to march 2000) have been summarized. two hundred seven cases were investigated, and 411 strains were isolated from 169 cases (81.6%). of those strains, 184 and 227 strains were from primary infections and postoperative infections, respectively. in p ...200212621728
[antibacterial activity of cefpodoxime against clinical isolates in 2000 and 2001].as the post-marketing surveillance of cefpodoxime proxetil (banan), mics of cefpodoxime (cpdx, an active form of banan) against 1090 clinical isolates of 22 species from 15 medical institutions all over japan from june 2000 to march 2001 were measured using the broth microdilution method approved by the japanese society of chemotherapy and compared with those of oral cephem antibacterials, cefaclor, cefdinir, cefditoren, and cefcapene. in this study, remarkable change in the activity of cpdx was ...200212621736
meningitis and shunt infection caused by anaerobic bacteria in children.this review describes the microbiology and management of meningitis and shunt infections caused by anaerobic bacteria in children. the predominant anaerobes recovered in meningitis are bacteriodes spp., bacteriodes fragilis, fusobacterium spp., and clostridium spp. peptostreptococcus, veillonella, actinomyces, propionibacterium acnes, and eubacterium are less commonly isolated. the predisposing conditions for meningitis are acute or chronic middle-ear infection, sinusitis, pharyngitis, and pulmo ...200211897473
bacterial meningoencephalomyelitis in dogs: a retrospective study of 23 cases (1990-1999).the clinical records of 23 dogs (1990-1999) with histopathologically confirmed bacterial meningoencephalomyelitis were evaluated retrospectively. no breed, age, sex, or weight predisposition was found. all the dogs presented with clinical signs of a brain lesion, whereas 5 of 23 had neck pain. pyrexia was detected in 11 of 23 dogs on admission. cbcs revealed neutrophilic leucocytosis in 7 of 21 dogs and thrombocytopenia in 3 of 21 dogs. the serum chemistry profiles were abnormal in 15 of 21 dogs ...200211899031
effect of deuteration on some structural parameters of methyl groups in proteins as evaluated by residual dipolar bond methyl 1h-13c and 13cmethyl-13c scalar and residual dipolar couplings have been measured at sites in an 15n, 13c, approximately 50% 2h labeled sample of the b1 immunoglobulin binding domain of peptostreptococcal protein l to investigate changes in the structure of methyl groups in response to deuterium substitution. both one bond methyl 1h-13c and 13cmethyl-13c scalar coupling constants have been found to decrease slightly with increasing deuterium content. previous studies have shown t ...200212061716
[clinical aspects of abscess development in parotitis].the purpose of this paper was to analyse parotideal abscesses on the basis of a larger number of patients.200212063691
acute suppurative thyroiditis caused by an infected piriform sinus fistula with thyrotoxicosis.we report herein an unusual case of thyrotoxicosis caused by acute suppurative thyroiditis (ast) infected through a piriform sinus fistula (psf). a 28-year-old man presented with pain over the thyroid gland and elevated serum thyroid hormone levels, a picture similar to subacute thyroiditis. a fine-needle aspiration biopsy from the left lobe showed neutrophil infiltration, and culture from the aspirate grew anaerobic peptostreptococcus. a neck computed tomography (ct) scan showed an abscess in t ...200211916288
[yearly changes in antibacterial activities of cefozopran against various clinical isolates between 1996 and 2000--i. gram-positive bacteria].the in vitro antibacterial activities of cefozopran (czop), an agent of cephems, against various clinical isolates obtained between 1996 and 2000 were yearly evaluated and compared with those of other cephems, oxacephems, carbapenems, and penicillins. fifteen species, 1,062 strains, of gram-positive bacteria were isolated from the clinical materials annually collected from january to december, and consisted of methicillin-susceptible staphylococcus aureus (mssa; n = 127), methicillin-resistant s ...200212071093
management of acute suppurative parotitis. 200211928106
antimicrobial effects of 2.94 microm er:yag laser radiation on root surfaces: an in vitro study.this in vitro study investigated the antimicrobial effects of 2.94 microm er:yag laser radiation on root surfaces.200211846852
[retrospective analysis of fusobacterium associated infections; experience at aomori prefectural hospital with 108 cases from 1995 to 1999].we experienced 108 cases of fusobacterium associated infections, including otolaryngeal, oral, pleuropulmonary, intraabdominal, skin and soft tissue infections, at aomori prefectural hospital during the 5 year-period from 1995 to 1999. a total of 433 organisms, included 113 fusobacterium spp. (80 fusobacterium nucleatum, 18 fusobaterium necrophorum, 5 fusobacterium varium, 4 fusobacterium mortiferum, 6 fusobacterium spp.), were recovered with an average of 4.0 organisms per case of the 108 cases ...200211852471
effects of instrumentation, irrigation and dressing with calcium hydroxide on infection in pulpless teeth with periapical bone lesions.the aim of this study was to evaluate the fate of microorganisms in root canals of teeth with infected pulps and periapical bone lesions with and without the use of calcium hydroxide medication.200211858203
in vitro activity of gemifloxacin compared to seven other oral antimicrobial agents against aerobic and anaerobic pathogens isolated from antral sinus puncture specimens from patients with sinusitis.using a microbroth method for aerobes and agar dilution for anaerobes, we studied the comparative in vitro activity of gemifloxacin, three fluoroquinolones, two macrolides and two beta-lactams, against 207 aerobic and 162 anaerobic antral sinus puncture isolates. gemifloxacin was active at < or = 0.25 ug/ml against 198/207 [96%] aerobes and 127/162 [78%] anaerobes or 325/369 [88%] of all isolates and was the most active quinolone on a weight basis against gram-positive organisms. all haemophilus ...200211858906
design and evaluation of oligonucleotide-microarray method for the detection of human intestinal bacteria in fecal oligonucleotide-microarray method was developed for the detection of intestinal bacteria in fecal samples collected from human subjects. the 16s rdna sequences of 20 predominant human intestinal bacterial species were used to design oligonucleotide probes. three 40-mer oligonucleotides specific for each bacterial species (total 60 probes) were synthesized and applied to glass slides. cyanine5 (cy5)-labeled 16s rdnas were amplified by polymerase chain reaction (pcr) from human fecal samples or ...200212167534
periodontal bacteria in rabbit mandibular and maxillary abscesses.despite the high incidence of odontogenic abscesses in pet rabbits, published data on the bacteriology of these infections are lacking, and clinical cultures are often ambiguous, making antibiotic choices difficult. in order to define the bacteriology of these infections, 12 rabbit mandibular and maxillary abscesses were cultured aerobically and anaerobically. all specimens yielded pathogenic bacteria, including fusobacterium nucleatum, prevotella heparinolytica, prevotella spp., peptostreptococ ...200211880435
differences in backbone dynamics of two homologous bacterial albumin-binding modules: implications for binding specificity and bacterial adaptation.proteins g and pab are bacterial albumin-binding proteins expressed at the surface of group c and g streptococci and peptostreptococcus magnus, respectively. repeated albumin-binding domains, known as ga modules, are found in both proteins. the third ga module of protein g from the group g streptococcal strain g148 (g148-ga3) and the second ga module of protein pab from p.magnus strain alb8 (alb8-ga) exhibit 59% sequence identity and both fold to form three-helix bundle structures that are very ...200211884146
[post-marketing surveillance of antibacterial activities of cefozopran against various clinical isolates--i. gram-positive bacteria].as a post-marketing surveillance, the in vitro antibacterial activities of cefozopran (czop), an agent of cephems, against various clinical isolates were yearly evaluated and compared with those of other cephems, oxacephems, penicillins, and carbapenems. changes in the bacterial sensitivity for czop were also evaluated with the resistance ratio calculated with breakpoint mic. sixteen species (1,913 strains) of gram-positive bacteria were isolated from the clinical materials annually collected fr ...200211977919
comparative in vitro activity of faropenem and 11 other antimicrobial agents against 405 aerobic and anaerobic pathogens isolated from skin and soft tissue infections from animal and human bites.faropenem, a new oral beta-lactam agent with a penem structure, was very active against 405 aerobic and anaerobic bite isolates. it inhibited 232 of 236 (98%) aerobic isolates, including all pasteurella spp. and eikenella corrodens at < or = 0.25 mg/l, and 164/169 (97%) anaerobic isolates, at < or = 1 mg/l. the 10 isolates that required > or = 2 mg/l for inhibition were one strain each of acinetobacter lwoffi, corynebacterium minutissimum, bacteroides ovatus, lactobacillus delbrueckii and peptos ...200212205068
bacteriology and antimicrobial susceptibility of gram-positive cocci isolated from pus specimens of orofacial odontogenic infections.we recently reported the beta-lactamase production and antimicrobial susceptibility of anaerobic gram-negative rods isolated from pus specimens of 93 orofacial odontogenic infections. in this report, we determine the bacteriology and antimicrobial susceptibility of bacteria other than anaerobic gram-negative rods, mainly gram-positive cocci, isolated from the same specimens. streptococcus constellatus and peptostreptococcus micros were frequent isolates from all types of infection examined. pept ...200211929563
subgingival microbiota of indigenous indians of central define the subgingival microbial profiles of adult subjects from a previously identified rural community of indigenous indians in guatemala, central america.200211895544
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